Virus diversity and evolution

Lead Research Organisation: The Pirbright Institute
Department Name: UNLISTED

Abstract

The Pirbright Institute provides the UK with capacity to predict, detect, understand and respond to incursions of viral pathogens of livestock and viruses that spread from animals to humans. Pirbright represents a hub of world class facilities and expertise for the study of exotic and endemic viral diseases in the natural host, therefore enabling our research to be directly translated into future control measures.
Virus genomes are continuously reshaped by mutation, recombination and re-assortment. These processes facilitate the diversity and rapid evolution required for virus adaptation to selection pressures in their environment. Exploiting recent advances in genomic sequencing, this project will address a series of common themes to define the mechanisms that drive the evolution of the diverse range of pathogenic mammalian and avian viruses that are studied at Pirbright. These represent viruses with RNA (positive-sense, negative-sense and segmented) and DNA genomes. At the finest scales, we will exploit the latest techniques for sequencing viral genomes to characterise RNA viral swarms as they are transmitted within and between susceptible hosts. At a broad scale, studies will be undertaken to provide valuable insights into the factors that are responsible for the natural extent of viral sequence variation that is generated by the circulation of the viruses in endemic settings. Building upon our international expertise in this area, these studies will provide crucial information that underpins our knowledge of the global epidemiology of these diseases and is essential for disease-prediction models and the development of successful vaccines and antivirals. These data also have tremendous practical application as demonstrated by the development of sensitive molecular tests for foot-and-mouth disease virus (FMDV) that allow for more rapid testing of infected animals and reliable diagnosis of pre-clinically infected animals, resulting in improved disease control with culling of fewer animals. The work in this project will be made possible by close interaction with the diagnostic facilities at Pirbright which act as UK, European and World Reference Laboratories with unrivalled access to physical samples and sequence databases. Anticipated outcomes of this project are: improved fundamental understanding of the mechanisms of virus evolution and the relationships between evolution, pathogenesis and epidemiology; knowledge of how virus evolution is shaped by natural and vaccine derived immune responses will inform future vaccination strategies; provision of web based tools for rapid and open dissemination of viral sequence data and tools for rapid characterisation of novel/unexpected disease syndromes; information to inform forecasting models for disease severity, prevalence, transmission and spread.

Organisations

Publications

10 25 50
 
Description This project is part of the BBSRC Institute Strategic Programme "Understanding and preventing viral diseases". Progress towards the five objectives is described below:

[1] Development of cost-effective NGS protocols
A particular focus of recent work has been to develop full genome sequencing protocols to better understand the genetic diversity of African swine fever virus (ASFV) that is currently causing outbreaks in Europe and Asia.

[2] Understanding the role of error rates/swarms in determining viral phenotype
We have recently developed faster and more sensitive analytical tools to detect variants in viral quasispecies. Our simplified Bayesian approach can be applied to deep-sequencing data (even at very high coverage) computes the posterior probability that a variant is not generated by sequencing errors or PCR artefacts.

[3] Defining the influence of recombination/re-assortment on virus evolution
For foot-and-mouth disease virus (FMDV), we have established new approaches to detect recombination events between closely related viral sequences. Recombination rates for samples collected from African Buffalo infected with a serotype SAT-1 virus containing two sub-populations (differing in their capsid-coding sequences) were much higher than expected. Although these intra-host results are not immediately translatable to global phylogenetics, they indicate that recombination and epistasis play an important yet underappreciated role in the evolution of FMDV.

[4] Understanding transmission bottlenecks and host immune pressure on viruses
We have investigated the process of attenuation for infectious bronchitis virus (IBV) in eggs, where large genetic bottlenecks can severely restrict viral diversity, from which viral populations do not always recover. This information has relevance to the development of novel vaccines to newly emerging coronaviruses e.g. SARS-CoV-2. We have also applied new tools to show that FMDV inter-host bottlenecks are a similar size to intra-host bottlenecks. Immune-mediated evolutionary drivers of FMDV and infectious bursal disease virus (IBDV) are being studied using an in vitro model that uses sub-neutralising antibody concentrations to drive amino acid substitutions that confer different antigenic phenotypes. Furthermore, our work with H9N2 avian influenza (AIV) virus suggests that some viruses are pre-disposed to rapid adaptation in mammalian hosts via changes in the viral polymerase. Other research has defined underlying mechanisms that drive vaccine induced immunity of AIV H7 and H9 in poultry. These results provide baseline data for future vaccine development and seed strain assessment by providing quantitative molecular markers that can help explain vaccine breakdown in the field and predict levels of antigenic drift during virus surveillance.

[5] Exploiting viral sequence data from our unique sample archive
Linking to Objective 3, we have identified two novel genotypes of FMDV which may represent isolates which have been subjected to geographical isolation within Africa by the Great African Rinderpest Pandemic (1887-1897). These findings provide insights into the un-sampled genetic variability that may exist within buffalo, which are natural reservoirs for FMDV in Africa. We have also established that the AIV accessory protein, PB1-F2 can exist as a N-terminally truncated protein that persists in avian hosts, suggesting that the C-terminus is important for viral fitness.
Exploitation Route Understanding the processes that drive genetic and antigenic viability for RNA viruses have broad impacts upon the implementation of disease control policies as well as specific activities to develop tailored tools (such as vaccines) to control these diseases.
Sectors Agriculture, Food and Drink,Education,Environment,Healthcare,Pharmaceuticals and Medical Biotechnology

 
Description One important output from this project has been to characterise the ecology and molecular epidemiology of H5, H7 and H9 avian influenza viruses that have significant impacts upon poultry production and a potential for zoonotic transmission. The findings from this work have helped the poultry-vaccine producers to start to design more broadly protective vaccines against antigenically divergent H5, H7 and H9 avian influenza. Production of effective vaccines by industry using research data will reduce avian influenza virus prevalence in poultry also reduced zoonotic transmission to humans.
First Year Of Impact 2018
Sector Agriculture, Food and Drink,Healthcare,Pharmaceuticals and Medical Biotechnology
Impact Types Societal,Economic,Policy & public services

 
Description Contributing to the chapter on Marek's disease for the OIE Terrestrial Manual
Geographic Reach Multiple continents/international 
Policy Influence Type Implementation circular/rapid advice/letter to e.g. Ministry of Health
Impact The contribution to the Terrestrial Manual on the recent advances in the diagnosis and control of Marek's disease will impact on improved and better control of the disease to reduce losses and improved food security
URL http://www.oie.int/
 
Description Contribution to a POSTnote on 'Reducing UK Antibiotic Use in Animals'
Geographic Reach National 
Policy Influence Type Participation in a advisory committee
 
Description Development and Evaluation of PPR DIVA vaccines
Geographic Reach Multiple continents/international 
Policy Influence Type Citation in other policy documents
Impact Peste des petits ruminants (PPR) is a highly contagious disease, severely affects small ruminants in almost 70 countries in Africa, the Middle East and parts of Asia. It causes USD 1.5 to 2 billion in losses each year in regions that are home to over 80% of the world's sheep and goats and to more than 330 million of the world's poorest people, many of whom depend on them for their livelihoods. The estimated current expenditure on PPR vaccination ranges between USD 270 and 380 million. The annual impact of PPR alone may be valued at between USD 1.45 and 2.1 billion per year. Approximately a third of the global financial burden of PPR is borne by Africa, with a further quarter borne by South Asia. This burden will be removed with the successful eradication of PPR. The undiscounted costs for a fifteen-year Global control Strategy of FAO and OIE are between USD 7.6 and 9.1 billion, with the first five years costing between USD 2.5 and 3.1 billion. (http://www.fao.org/3/a-i4460e.pdf). PPR is currently controlled by vaccination using mainly two live attenuated PPRV vaccines (Nigeria 75/1 and Sungri 96). However, the current vaccines and serological tests are unable to enable Differentiation between naturally Infected and Vaccinated Animals (DIVA). This factor precludes meaningful assessment of vaccine coverage and epidemiological surveillance based on serology, in turn reducing the efficiency of control programmes. Therefore, it is almost impossible to assess the quality and efficacy of existing PPR vaccines without knowing whether positive animals are vaccinated or naturally infected. Unlike rinderpest, where cattle and buffalo were primary hosts, in PPR, new crops (about 30-40%) of lambs and kids are produced every year and are the most susceptible population to bring back new PPR outbreaks6&7. Therefore, it is likely that the cycle of vaccinations and PPR outbreaks will continue until and unless we reach the stage of 80-90% herd immunity. Therefore, development of a marker vaccine and associated DIVA diagnostics will enable the assessment of vaccine efficacy which is essential for any successful vaccination campaign. https://www.pirbright.ac.uk/news/2018/09/pirbright-scientists-run-vaccination-campaign-eradicate-peste-des-petits-ruminant The availability of a recombinant PPRV vaccine with a proven functionality is a prerequisite for the development of novel vaccines that may enable the development of DIVA tools for PPRV diagnostics. In the DBT-BBSRC FADH BB/L004801/1 grant we have rescued Sungri 96 and Nigeria 75/1 vaccine strains. Both the vaccine strains were rescued from respective synthetic c-DNA clones with mutations in the variable part of C terminus of the nucleocapsid (N) gene similar to Dolphin Morbillivirus (DMV) to enable negative marker DIVA vaccines. These two DIVA vaccines along with parental vaccines have been recently tested in a pilot studies in goats. Both the DIVA and parent vaccines provided safety, stability and protection for vaccinated goats whereas the control animals were clinically infected. Patent applications have been made to protect these DIVA vaccines. Agreements are being done with vaccine industries for the licensing and commercialisation.
URL https://www.pirbright.ac.uk/press-releases/2018/09/pirbright-collaboration-provides-tools-peste-des-...
 
Description Drafted resolution that was endorsed by the World Assembly of Delegates (for the World Oganisation of Animal Health - OIE)
Geographic Reach Multiple continents/international 
Policy Influence Type Citation in other policy documents
Impact This policy document relates to one of the FMDV serotypes (called serotype C) which has disappeared for >10 years from the surveillance activities of the global OIE/FAO FMD Reference Laboratories (led by the Pirbright Institute). These laboratories now consider that the production of FMDV serotype C vaccines and their use in vaccine challenge experiments represent a risk of virus escape. Based on this evidence and concerns, this resolution urges that OIE Member Countries and vaccine manufacturers to stop the use of FMDV serotype C in vaccine challenge experiments and to consider halting the production of FMDV serotype C vaccines and inclusion in multivalent FMD vaccines except for holding in vaccine banks.
URL http://www.oie.int/fileadmin/Home/eng/About_us/docs/pdf/Session/2017/A_RESO_2017_Public.pdf
 
Description FMD: Republic of Korea
Geographic Reach Asia 
Policy Influence Type Implementation circular/rapid advice/letter to e.g. Ministry of Health
 
Description Organised seminar series on Sequencing, Virus Evolution and Molecular Biology at Pirbright (with Luca Ferretti and Graham Freimanis)
Geographic Reach Local/Municipal/Regional 
Policy Influence Type Influenced training of practitioners or researchers
 
Description PPR Global Research and Expertise Network (PPR GREN) membership (OIE/FAO)
Geographic Reach Multiple continents/international 
Policy Influence Type Participation in a advisory committee
 
Description Participation in the Management of the Oxford DTP
Geographic Reach Multiple continents/international 
Policy Influence Type Influenced training of practitioners or researchers
Impact As part of the Management committee of the Oxford-Pirbright-Oxford Brookes-Diamond doctorate Training Partnership, contributed to the success of the DTP in meeting the objectives, recruitment and review
URL http://www.biodtp.ox.ac.uk/
 
Description Taught course on introductory bioinformatics to PhD students at Pirbright
Geographic Reach Local/Municipal/Regional 
Policy Influence Type Influenced training of practitioners or researchers
 
Description Training MSc students
Geographic Reach Local/Municipal/Regional 
Policy Influence Type Influenced training of practitioners or researchers
Impact Delivered a new training session to MSc students teaching viral quantification assays. This lead to a greater understanding of laboratory techniques and academic research.
 
Description A One Health approach to pan-valent morbillivirus vaccines
Amount £773,237 (GBP)
Funding ID BB/R019843/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 05/2018 
End 03/2021
 
Description BBSRC Industrial CASE (iCASE)
Amount £100,000 (GBP)
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 10/2019 
End 09/2022
 
Description British Egg Marketing Board PhD studentship
Amount £76,000 (GBP)
Organisation British Egg Marketing Board 
Sector Private
Country United Kingdom
Start 10/2018 
End 09/2022
 
Description Commonwealth Scholarship. Molecular determinants impacting avian influenza H9N2 virus evolution, replication fitness and virulence
Amount £200,000 (GBP)
Funding ID LKCS-2019- 665 
Organisation British Council 
Sector Charity/Non Profit
Country United Kingdom
Start 11/2019 
End 10/2022
 
Description Enhancing protective efficacy of avian influenza vaccines through targeted delivery of protective antigens to chicken immune cells
Amount £98,212 (GBP)
Funding ID BB/R50595X/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 09/2017 
End 09/2021
 
Description Identification of interaction sites between the genomic segments of influenza virus as putative novel anti-viral targets
Amount £100,000 (GBP)
Organisation The Pirbright Institute 
Sector Academic/University
Country United Kingdom
Start 10/2019 
End 09/2024
 
Description Japan Partnering Award
Amount £49,500 (GBP)
Funding ID BB/P025803/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 09/2017 
End 08/2021
 
Description Newton Fund: UK-China-Philippines-Thailand Swine and Poultry Research Initiative
Amount £841,995 (GBP)
Funding ID BB/R012679/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 01/2018 
End 12/2020
 
Description Newton Fund: UK-China-Philippines-Thailand Swine and Poultry Research Initiative. Understanding antigenic diversity, zoonotic potential and immunological prevention of avian influenza viruses affecting poultry.
Amount £497,995 (GBP)
Funding ID BB/R012679/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 01/2018 
End 12/2020
 
Description OIE Twinning: Development of capacity for independent assessment of FMDV vaccines in Africa
Amount $1,466,186 (USD)
Organisation World Organisation for Animal Health 
Sector Public
Country France
Start 04/2019 
End 03/2021
 
Description Pirbright Institute Flexible Talent Mobility Account
Amount £180,000 (GBP)
Funding ID BB/S507945/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 11/2018 
End 03/2021
 
Description Reducing the Economic and Zoonotic Impact of Avian Influenza (REZIAI): delivering novel vaccines and diagnostics from laboratory to the field.
Amount £400,000 (GBP)
Funding ID BB/S013792/1 
Organisation The Pirbright Institute 
Sector Academic/University
Country United Kingdom
Start 02/2019 
End 01/2021
 
Description Responsive mode: Avian influenza H7N9 virus evolution: defining the impact of internal genes on virus infection in avian and mammalian species
Amount £668,314 (GBP)
Funding ID BB/N002571/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 07/2016 
End 06/2019
 
Description Responsive mode: Rapid acquisition of mammalian characteristics by avian influenza virus in single host infections.
Amount £535,337 (GBP)
Funding ID BB/R007292/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 06/2018 
End 01/2021
 
Description Taiwan Partnering Award
Amount £40,700 (GBP)
Funding ID BB/S020624/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start  
 
Description The Global Challenges Research Fund
Amount £640,473 (GBP)
Organisation Research Councils UK (RCUK) 
Sector Public
Country United Kingdom
Start 04/2019 
End 03/2024
 
Description The Pirbright Institute - University of Surrey PhD studentship
Amount £100,000 (GBP)
Organisation The Pirbright Institute 
Sector Academic/University
Country United Kingdom
Start 10/2019 
End 03/2023
 
Description Use of FMDV sequence data to trace outbreaks and monitor disease incursion risks to the UK
Amount £1,326,558 (GBP)
Funding ID SE2944 
Organisation Department For Environment, Food And Rural Affairs (DEFRA) 
Sector Public
Country United Kingdom
Start 04/2019 
End 03/2022
 
Description ZELS-AS: Molecular epidemiology of H9N2 avian influenza viruses in Vietnam and Pakistan.
Amount £100,000 (GBP)
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 10/2015 
End 09/2019
 
Description iCASE studentship: Production and assessment of antiviral prophylactic properties of natural biomolecules against avian influenza and Newcastle disease viruses affecting poultry
Amount £100,000 (GBP)
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 10/2019 
End 09/2023
 
Title Deep sequencing to identify RNA packaging signals 
Description A novel and simple approach to identify predicted RNA secondary structures involved in genome packaging in positive sense RNA viruses (e.g. the picornavirus foot-and-mouth disease virus [FMDV]). By interrogating deep sequencing data generated from both packaged and unpackaged populations of RNA, we have determined multiple regions of the genome with constrained variation in the packaged population. Predicted secondary structures of these regions revealed stem-loops with conservation of structure and a common motif at the loop. Disruption of these features resulted in attenuation of virus growth in cell culture due to a reduction in assembly of mature virions. This study provides evidence for the involvement of predicted RNA structures in picornavirus packaging and offers a readily transferable methodology for identifying packaging requirements in many other viruses. 
Type Of Material Technology assay or reagent 
Year Produced 2017 
Provided To Others? Yes  
Impact Impact so far is fundamental knowledge but with several potential avenues for future impact in for example improved vaccines. 
 
Title Detection of HA antigens of avian influenza viruses 
Description Assay for detection of recombinant HVT expressing HA antigens of avian influenza viruses 
Type Of Material Technology assay or reagent 
Year Produced 2018 
Provided To Others? Yes  
Impact This new method will help to detect recombinant HVT vaccine expressing HA antigen in infected cell. 
 
Title Development of CLEM techniques for RSV 
Description We have developed the technical expertise to perform correlative light electron microscopy on respiratory syncytial virus infected cells. This allows us to directly compare immunofluorescent images with transmission EM of the same cell. 
Type Of Material Technology assay or reagent 
Year Produced 2019 
Provided To Others? No  
Impact We have recently submitted a paper and grant building on this expertise, as well as forging new collaborations with Diamond light source. 
 
Title Method for sequencing of ASFV (African Swine Fever Virus) based on long reads 
Description The ASFV genome is repetitive, due to the presence of families of genes that are present in multiple copies (>10). As a result, sequencing based on short reads only would not be able to reconstruct the genome in its entirety. In addition, good quality full genome sequencing of ASFV is complicated by persistence contamination with fragments of host DNA. We are developing a novel protocol that will allow the sequencing of the virus using long-read technologies (PacBio or Oxford Nanopore). Probe-capture libraries have been designed to mitigate the problem of host contamination by selectively purifying viral DNA from a wide range of different ASFV genotypes. Protocols are being developed to prepare high quality high molecular weight viral DNA from different sample types suitable for generating sequencing libraries for long-read sequencing. 
Type Of Material Technology assay or reagent 
Year Produced 2019 
Provided To Others? No  
Impact More than 100 samples from 18 different LMIC countries have been identified and collated for sequencing with the new protocol. They will be added to the web-portal for transboundary pathogens which is currently being developed thanks to BBSRC funding. 
 
Title NGS for PPR virus whole genome sequencing 
Description We have finalised a protocol to sequence the whole genome of PPR virus 
Type Of Material Technology assay or reagent 
Year Produced 2017 
Provided To Others? Yes  
Impact The method has been described and shard with project partners and a draft has been written for publishing the methods 
 
Title Optimisation of chicken kidney cell culture preparation 
Description We have optimised the method of extracting kidneys and preparing chicken kidney (CK) cell cultures from chickens. CK cells are used for in vitro assays to study avian viruses, in particular infectious bronchitis virus. 
Type Of Material Cell line 
Year Produced 2017 
Provided To Others? No  
Impact This has increased the yield of viable cells and the quality of the cell cultures. We can now obtain the number of cells we require using fewer chicken kidneys, therefore requiring fewer chickens to be culled. This is an important development for the 3Rs. We hope to publish this method for others' information. 
 
Title Optimisation of preparation of TOCs from adult chickens 
Description Tracheal organ cultures (TOCs) are prepared from chicken embryos and used to assess growth of avian viruses that are not able to be propagated in cell culture. We have optimised the method of TOCs preparation from 3-week old chickens and are using these to assess viral genetic stability and phenotype in organ cultures that are a similar environment to the site of replication in whole chickens. 
Type Of Material Cell line 
Year Produced 2018 
Provided To Others? No  
Impact We hope that this technique will reduce the number of chickens required for in vivo experiments, in line with the 3Rs, and plan to publish the method in due course. 
 
Title FMDV LIMS and webtools 
Description The Integrative Biology and Bioinformatics (IBB) group has been supplying state-of-the-art bioinformatics tools to the World Reference Labs for the Foot-and-Mouth Disease Virus. All tools revolve around a database of genomic sequences and metadata for FMDV samples. Some tools allow members of the lab to enter, process and annotate new data into the database, generating several kinds of reports. Other web-based tools (the "FMDV Toolbox") allow external users to query the database in several ways. Bioinformatics workflows are hosted on the IBB cluster, and have been implemented as REST web services so as to be decoupled from the web interface. The web interface is currently being redeveloped with the help of a specialised company. 
Type Of Material Database/Collection of data 
Year Produced 2016 
Provided To Others? Yes  
Impact The reports generated by our system are used to inform FMDV policy-makers both within the UK and in the rest of the world. External users can compare their sequences to those contained in the database, and get useful biological insights. 
URL https://mallorn.pirbright.ac.uk/FMDVToolbox
 
Title GCRF portal on transboudary diseases 
Description The Integrative Biology and Bioinformatics group is developing a GCRF-funded Portal on Transboundary Diseases. It will be an easy way for all scientists, and those from LMI countries in particular, to access data about several viral diseases generated at Pirbright. The portal will be country-based, presenting the user with a map and dispatching them to the information relevant to the selected region. From that information the user will be able to reach specialised websites for diseases present in the country, and data generated at Pirbright (such as sequences for FMDV, several Capripox viruses, and ASFV). Sequences relevant to the portal are currently being generated. Bioinformatics workflows are hosted on the IBB cluster, and have been implemented as REST web services so as to be decoupled from the web interface. The web interface is currently being developed with the help of a specialised company. 
Type Of Material Database/Collection of data 
Year Produced 2018 
Provided To Others? No  
Impact The portal will benefit scientists from LMI countries, and all scientist, allowing them to access/download genetic and other data relevant to animal diseases present in a country of choice and studied and Pirbright. Some of the data (for instance the FMDV data generated by the World Reference Lab) is already use to inform policy at international level. Being able to access information in a systematic way will increase the potential for it to be put to good use. 
 
Title South-African buffalo FMDV sequences 
Description We have sequenced FMDV buffalo samples originated from a EEID project entitled: Persistence of a Highly Contagious Pathogen: Ecological and Evolutionary Mechanisms in Foot-And-Mouth Disease Virus. This project aims to understand why a highly contagious pathogen such as FMDV, which induces a rapid host immunity and depletes the supply of susceptible host, is able to persist in isolated buffalo populations and thus avoid auto-extinction. The centre piece of the project is a cohort study which involves an established FMDV-positive breeding herd of ca. 70 buffalo in a 900-hectare enclosure surrounded by double game fencing housing buffalo in isolation from other herds in the Kruger National Park (South Africa). The entire herd is being monitored for three years (animals are sampled every 2 months (serum, tonsil swabs, probang) to trace FMDV transmission events, allowing us to define FMD infection dynamics across the susceptible calf cohorts and amongst adults. So far, FMDV genomes from 101 samples have been deep sequenced by illumina. Samples from the last year captures are currently being analysed and virus is currently being isolated. The second experiment is an experimental study, which involves a group of naïve buffalo experimentally infected with either a SAT-1, SAT-2 or SAT-3 virus. The infected buffalo were then allowed to mingle with susceptible animals and transmission of FMDV to naïve animals was monitored during the acute infection but also from persistently infected animals. So far, FMDV genomes from 48 samples obtained at days 2, 30 and 160 of the experiment have been deep sequenced. 
Type Of Material Database/Collection of data 
Year Produced 2019 
Provided To Others? No  
Impact The samples sequenced come from South Africa, which is an LMIC, While FMDV infection in cattle is very well studied, the mechanisms of persistence in buffalo, which is thought to be the natural reservoir of the infection, are not well understood. Such understanding would be essential to inform better policies to understand and manage FMDV, which is an economically important scourge of cloven hooved animals in LMICs. All the sequences will be made available on the Transboundary Portal which is being developed at Pirbright. 
 
Title Viral assembly pipeline 
Description The Integrative Biology and Bioinformatics group has developed a data analysis pipeline to obtain sensitive and robust viral assemblies out of high-throughput sequencing data. In spite of the relatively short genomic length of most viruses, assembling of viral sequences can be challenging due to several reasons, such as: low amount of material in the sample, which might require amplification, introducing biases, and/or generates low-quality sequences; uneven coverage due to low-quality material or the genomic material of the virus being RNA; the viral nucleic acid being almost lost in the background of nucleic acid of the host. Our pipeline overcomes most of those problems, and is even able to detect different viral strains being present together in the same sample. It can also be used on metagenomic environmental samples. 
Type Of Material Data analysis technique 
Year Produced 2017 
Provided To Others? No  
Impact The pipeline is being deployed as the tool of choice for the nascent sequencing facility at Pirbright. It has been used by several groups at Pirbright, and the FMDV World Reference Lab, in order to assemble very different kinds of viruses. 
 
Title Viral variant calling pipeline 
Description The Integrative Biology and Bioinformatics group has developed a data analysis pipeline to obtain sensitive and robust variant calling for viral sequences out of high-throughput sequencing data. In spite of the relatively short genomic length of most viruses, calling variant for viral sequences can be challenging due to several reasons, such as: low amount of material in the sample, which might require amplification, introducing biases, and/or generates low-quality sequences; very high genome coverage, which slows down most existing variant callers; the presence of a potentially very high number of different haplotypes for RNA viruses, which is an unusual scenario in variant calling. Our fast and sensitive Bayesian pipeline overcomes most of those problems. 
Type Of Material Data analysis technique 
Year Produced 2017 
Provided To Others? No  
Impact The pipeline is being deployed as the tool of choice for the nascent sequencing facility at Pirbright. It has been used by several groups at Pirbright, and the FMDV World Reference Lab, in order to assemble very different kinds of viruses. 
 
Title WRL FMDV sequences for transboundary portal 
Description We have generated a collection of sequences of FMDV (foot-and-mouth disease virus) from samples collected by the FMDV World Reference Laboratory at Pirbright. Those full-genome sequences sample a number of recent exotic strains across different regions/countries of the world, focusing mainly on LMICs in North Africa, Middle East and South East Asia. The main represented FMDV types are O and A. Part of the sequences have already been made available through GenBank -- all will be soon downloadable from the Transboundary Portal which is being developed at Pirbright. 
Type Of Material Database/Collection of data 
Year Produced 2019 
Provided To Others? Yes  
Impact Knowledge of the sequenced strains is essential to inform policy for FMDV control in the LMIC countries where the samples were collected. That chimes very well with the GCRF focus of the project. At a more global scale, knowledge of the features and sequence evolution of the virus in time and across different countries is essential in order to accurately model its behaviour. In general FMDV is a scourge of cloven-hoofed animals, and controlling it better is essential in order to alleviate the huge economic toll claimed by the virus in LMICs. 
 
Title Zimbabwe FMDV sequences 
Description Foot-and-mouth disease virus (FMDV) causes an acute vesicular disease in domestic cloven-hooved animals. However, in the African buffalo (Syncerus caffer) clinical disease is rarely observed and following infection virus is persistently carried in the oesophageal-pharyngeal area of the upper respiratory tract. During the 1990s oesophageal-pharyngeal scrapings were collected from free-living African buffalo in multiple herds in six different geographic areas of Zimbabwe. We sequenced over 140 FMD viruses each belonging to one of the Southern African Territories (SAT) serotypes (SAT 1, SAT 2 and SAT 3) from primary bovine thyroid cells. 
Type Of Material Database/Collection of data 
Year Produced 2018 
Provided To Others? No  
Impact This dataset has been generated from samples collected in Zimbabwe, which is a low-income country. Those viral sequences will be essential to help elucidating the nature of persistent FMDV infection in African buffalo, which is supposed to be the main virus reservoir in vivo. As the samples track viral infection and evolution over several years and across a number of different herds in different national parks and conservatories, the dataset will also inform better animal management and conservation. A better understanding of FMDV persistence would also be essential to mitigate the economic burden generated by the disease, which is a scourge of cloven-hoofed animals in LMICs. All the sequences will be made available through the Transboundary Pathogen portal that is being developed at Pirbright. 
 
Description Anihwa Call 1 
Organisation Agricultural Research for Development (CIRAD)
Country France 
Sector Private 
PI Contribution Developed PCR to detect PPR viral genome in faecal samples for experimentally infected animals.
Collaborator Contribution Developed PCR and antigen ELISA to detect PPR viral genome in faecal samples for field animals including wild life.
Impact Please see publications
Start Year 2013
 
Description Anihwa Call 1 
Organisation Friedrich Loeffler Institute
Country Germany 
Sector Academic/University 
PI Contribution 1. Conducted challenge experiments in goats to study pathogenicity. 2. Developed NGS technology to sequence PPR whole genome.
Collaborator Contribution FLI has conducted transmission study between different species ( Goats, pig, camel). For the first time they showed that pigs are clinically infected by PPR virus.
Impact Joint Publications
Start Year 2013
 
Description Anihwa Call 1 
Organisation International Atomic Energy Agency
Country Austria 
Sector Charity/Non Profit 
PI Contribution 1. Phylogenetic analysis and whole genome sequencing
Collaborator Contribution 1.Phylogenetic analysis and whole genome sequencing
Impact Joint publications
Start Year 2013
 
Description Anihwa Call 1 
Organisation Kimron Veterinary Institute
Country Israel 
Sector Charity/Non Profit 
PI Contribution 1.Analysed evolution of PPR viruses from 1993 to 2017 circulating in Israel by conducting whole genome sequencing and Bayesian analysis .
Collaborator Contribution Submitted virus to Pirbright
Impact Joint publications
Start Year 2013
 
Description Annika Posautz; University of Veterinary Medicine Vienna. Morbillivirus host-diversity analysis. 
Organisation University of Veterinary Medicine Vienna
Country Austria 
Sector Academic/University 
PI Contribution Sharing of research data regarding morbillivirus host-range and technical support to the project.
Collaborator Contribution Provision of tissue samples from potential morbillivirus hosts, e.g. carnivores.
Impact No outcomes as yet.
Start Year 2018
 
Description Avian influenza H7N9 virus evolution: defining the impact of internal genes on virus infection in avian and mammalian species 
Organisation Animal and Plant Health Agency
Country United Kingdom 
Sector Public 
PI Contribution Provision of samples and reagents for research
Collaborator Contribution Provision of samples, reagents for research and research facilities
Impact Research output entitled "A restriction digestion and ligation independent technique for cloning influenza gene segments into pHW2000" was presented at January 17th to 19th 2018. Fortuna Hotel, Lang Ha Street, Hanoi, VietNam (Presented by postdoctoral scientist Dr Sushant Bhat
Start Year 2016
 
Description Avian influenza H7N9 virus evolution: defining the impact of internal genes on virus infection in avian and mammalian species 
Organisation Francis Crick Institute
Country United Kingdom 
Sector Academic/University 
PI Contribution (1) Exchange of research methods and reagents
Collaborator Contribution (1) Exchange of research methods and reagents. (2) Provision of training to PhD students and postdoctoral Scientist (3) Critical review of experimental data and manuscripts.
Impact (1) Joint funding: BBSRC Newton Fund: UK-China-Philippines-Thailand Swine and Poultry Research Initiative (2018-2021). Understanding antigenic diversity, zoonotic potential and immunological prevention of avian influenza viruses affecting poultry. Value: £497,995.93. UK Co-Is: Prof John McCauley (Crick Institute), Dr Richard Reeve (University of Glasgow). Collaborators from China: Dr Yipeng Sun, Prof Jinhua Liu and Dr Juan Pu (China Agricultural University), Prof Yongqing Li (Beijing Academy of Agriculture and forestry Sciences), Dr Wenfei Zhu (National Institute for Viral Disease Control and Prevention, China CDC).
Start Year 2007
 
Description BBSRC - Oxford University iCASE Studentship (October 2019- September 2023). Production and assessment of antiviral prophylactic properties of natural biomolecules against avian influenza and Newcastle disease viruses affecting poultry production 
Organisation University of Oxford
Country United Kingdom 
Sector Academic/University 
PI Contribution This collaboration between The Pirbright Institute (UK), Oxford University (UK) and industrial partners in Italy. This project will conceived and developed bu MI. The PhD student (Holly Everest) in my group is investigating the antiviral prophylactic properties of natural biomolecules against avian influenza and Newcastle disease viruses affecting poultry production.
Collaborator Contribution The partners in Italy will produce and purify natural antiviral molecules effective against avian influenza and other poultry viruses.
Impact This project started in January 2020.
Start Year 2020
 
Description Commonwealth Scholarship. Molecular determinants impacting avian influenza H9N2 virus evolution, replication fitness and virulence 
Organisation Royal Veterinary College (RVC)
Country United Kingdom 
Sector Academic/University 
PI Contribution I develop a research project proposal in which we are investigating how evolutionary changes in the H9N2 avian influenza virus modulate virus fitness and virulence in chickens. This PhD studentship project was funded by Commonwealth scholarship commission for 3 years.
Collaborator Contribution The collaborating Partner Dr Nicola Lewis at Royal Veterinary College is a co-supervisor of the PhD student Thusitha Karunarathna. she provide necessary guidance to achieve prescribed objectives of this project.
Impact The project is at its initial stage.
Start Year 2019
 
Description Development of novel diagnostic tools and vaccines 
Organisation Francis Crick Institute
Country United Kingdom 
Sector Academic/University 
PI Contribution The outcomes of research led to incite new collaborations with following partners in UK, Vietnam and Pakistan. Together with partners we apply for the collaborative research grant to BBSRC entitled "Combating avian influenza through systematic analysis of antigenic drift, genetic variation, and development of novel diagnostic tools and vaccines". That led to further improvement avian influenza virus vaccines and diagnostics and a number of research publication.
Collaborator Contribution The partners are evaluating the potency and efficacy of vaccines in countries where the disease is enzootic in poultry.
Impact 1. Peacock TP, Benton DJ, Sadeyen J-R, Chang P, Sealy JE.,d, Bryant JE, Martin SR., Shelton H, McCauley JW, Barclay WS, Iqbal M (2017) Variability in H9N2 haemagglutinin receptor binding preference and pH of fusion. Emerging Microbes & Infections 6, e11 2. Peacock T, Reddy K, James J, Adamiak B, Barclay W, Shelton H, Iqbal M (2016). Antigenic mapping of an H9N2 avian influenza virus reveals two discrete antigenic sites and a novel mechanism of immune escape. Scientific Reports 5: 18745. doi: 10.1038/srep18745. 3. Thuy DM, Peacock TP., Bich VTN, . Iqbal M, Juliet E. Bryant (2016). Prevalence and diversity of H9N2 avian influenza in chickens of Northern Vietnam, 2014. Infection, Genetics and Evolution 44: 530-540
Start Year 2014
 
Description Development of novel diagnostic tools and vaccines 
Organisation Imperial College London
Department School of Public Health
Country United Kingdom 
Sector Academic/University 
PI Contribution The outcomes of research led to incite new collaborations with following partners in UK, Vietnam and Pakistan. Together with partners we apply for the collaborative research grant to BBSRC entitled "Combating avian influenza through systematic analysis of antigenic drift, genetic variation, and development of novel diagnostic tools and vaccines". That led to further improvement avian influenza virus vaccines and diagnostics and a number of research publication.
Collaborator Contribution The partners are evaluating the potency and efficacy of vaccines in countries where the disease is enzootic in poultry.
Impact 1. Peacock TP, Benton DJ, Sadeyen J-R, Chang P, Sealy JE.,d, Bryant JE, Martin SR., Shelton H, McCauley JW, Barclay WS, Iqbal M (2017) Variability in H9N2 haemagglutinin receptor binding preference and pH of fusion. Emerging Microbes & Infections 6, e11 2. Peacock T, Reddy K, James J, Adamiak B, Barclay W, Shelton H, Iqbal M (2016). Antigenic mapping of an H9N2 avian influenza virus reveals two discrete antigenic sites and a novel mechanism of immune escape. Scientific Reports 5: 18745. doi: 10.1038/srep18745. 3. Thuy DM, Peacock TP., Bich VTN, . Iqbal M, Juliet E. Bryant (2016). Prevalence and diversity of H9N2 avian influenza in chickens of Northern Vietnam, 2014. Infection, Genetics and Evolution 44: 530-540
Start Year 2014
 
Description Development of novel diagnostic tools and vaccines 
Organisation National Agricultural Research Centre
Country Pakistan 
Sector Public 
PI Contribution The outcomes of research led to incite new collaborations with following partners in UK, Vietnam and Pakistan. Together with partners we apply for the collaborative research grant to BBSRC entitled "Combating avian influenza through systematic analysis of antigenic drift, genetic variation, and development of novel diagnostic tools and vaccines". That led to further improvement avian influenza virus vaccines and diagnostics and a number of research publication.
Collaborator Contribution The partners are evaluating the potency and efficacy of vaccines in countries where the disease is enzootic in poultry.
Impact 1. Peacock TP, Benton DJ, Sadeyen J-R, Chang P, Sealy JE.,d, Bryant JE, Martin SR., Shelton H, McCauley JW, Barclay WS, Iqbal M (2017) Variability in H9N2 haemagglutinin receptor binding preference and pH of fusion. Emerging Microbes & Infections 6, e11 2. Peacock T, Reddy K, James J, Adamiak B, Barclay W, Shelton H, Iqbal M (2016). Antigenic mapping of an H9N2 avian influenza virus reveals two discrete antigenic sites and a novel mechanism of immune escape. Scientific Reports 5: 18745. doi: 10.1038/srep18745. 3. Thuy DM, Peacock TP., Bich VTN, . Iqbal M, Juliet E. Bryant (2016). Prevalence and diversity of H9N2 avian influenza in chickens of Northern Vietnam, 2014. Infection, Genetics and Evolution 44: 530-540
Start Year 2014
 
Description Development of novel diagnostic tools and vaccines 
Organisation University of Oxford
Department Oxford University Clinical Research Unit Vietnam (OUCRU)
Country Viet Nam 
Sector Academic/University 
PI Contribution The outcomes of research led to incite new collaborations with following partners in UK, Vietnam and Pakistan. Together with partners we apply for the collaborative research grant to BBSRC entitled "Combating avian influenza through systematic analysis of antigenic drift, genetic variation, and development of novel diagnostic tools and vaccines". That led to further improvement avian influenza virus vaccines and diagnostics and a number of research publication.
Collaborator Contribution The partners are evaluating the potency and efficacy of vaccines in countries where the disease is enzootic in poultry.
Impact 1. Peacock TP, Benton DJ, Sadeyen J-R, Chang P, Sealy JE.,d, Bryant JE, Martin SR., Shelton H, McCauley JW, Barclay WS, Iqbal M (2017) Variability in H9N2 haemagglutinin receptor binding preference and pH of fusion. Emerging Microbes & Infections 6, e11 2. Peacock T, Reddy K, James J, Adamiak B, Barclay W, Shelton H, Iqbal M (2016). Antigenic mapping of an H9N2 avian influenza virus reveals two discrete antigenic sites and a novel mechanism of immune escape. Scientific Reports 5: 18745. doi: 10.1038/srep18745. 3. Thuy DM, Peacock TP., Bich VTN, . Iqbal M, Juliet E. Bryant (2016). Prevalence and diversity of H9N2 avian influenza in chickens of Northern Vietnam, 2014. Infection, Genetics and Evolution 44: 530-540
Start Year 2014
 
Description Development of novel diagnostic tools and vaccines 
Organisation University of Veterinary & Animal Sciences
Country Pakistan 
Sector Academic/University 
PI Contribution The outcomes of research led to incite new collaborations with following partners in UK, Vietnam and Pakistan. Together with partners we apply for the collaborative research grant to BBSRC entitled "Combating avian influenza through systematic analysis of antigenic drift, genetic variation, and development of novel diagnostic tools and vaccines". That led to further improvement avian influenza virus vaccines and diagnostics and a number of research publication.
Collaborator Contribution The partners are evaluating the potency and efficacy of vaccines in countries where the disease is enzootic in poultry.
Impact 1. Peacock TP, Benton DJ, Sadeyen J-R, Chang P, Sealy JE.,d, Bryant JE, Martin SR., Shelton H, McCauley JW, Barclay WS, Iqbal M (2017) Variability in H9N2 haemagglutinin receptor binding preference and pH of fusion. Emerging Microbes & Infections 6, e11 2. Peacock T, Reddy K, James J, Adamiak B, Barclay W, Shelton H, Iqbal M (2016). Antigenic mapping of an H9N2 avian influenza virus reveals two discrete antigenic sites and a novel mechanism of immune escape. Scientific Reports 5: 18745. doi: 10.1038/srep18745. 3. Thuy DM, Peacock TP., Bich VTN, . Iqbal M, Juliet E. Bryant (2016). Prevalence and diversity of H9N2 avian influenza in chickens of Northern Vietnam, 2014. Infection, Genetics and Evolution 44: 530-540
Start Year 2014
 
Description Distributed Bio - Gates foundation funding - universal influenza vaccine 
Organisation Distributed Bio.
Country United States 
Sector Private 
PI Contribution Pirbright has provided expert advice to the design of adjuvant and challenge studies in appropriate animal models for human influenza vaccine trials. Pirbright is facilitating these studies in line with UK home office requirements and good scientific practice.
Collaborator Contribution Distributed Bio have developed a novel vaccine that is hoped to be a candidate for a human universal influenza virus vaccine.
Impact Funding from Bill and Melinda Gates foundation as part of the Grand challenges - Universal vaccine for influenza for the animal studies involving Pirbright and Distributed Bio
Start Year 2019
 
Description Enhancing protective efficacy of avian influenza vaccines through targeted delivery of protective antigens to chicken immune cells 
Organisation University of Oxford
Department Department of Zoology
Country United Kingdom 
Sector Academic/University 
PI Contribution Enhancing protective efficacy of avian influenza vaccines through targeted delivery of protective antigens to chicken immune cells ( PhD student training.
Collaborator Contribution Training of next generation of Scientists (PhD student training).
Impact Training of next generation of Scientists (PhD student training).
Start Year 2017
 
Description Glasgow DH 
Organisation University of Glasgow
Department Institute of Cardiovascular and Medical Sciences
Country United Kingdom 
Sector Academic/University 
PI Contribution Joint supervision of PhD student
Collaborator Contribution Joint supervision of PhD student
Impact PhD completed. Papers published or in preparation.
Start Year 2013
 
Description Identification of conserved B-cell epitopes of highly pathogenic coronaviruses for broadspectrum immunotherapy and vaccine design. 
Organisation Imperial College London
Country United Kingdom 
Sector Academic/University 
PI Contribution We have performed experiments to generate preliminary data for a collaborative grant application, contributed to the writing of the grant proposal and responded to reviewers comments.
Collaborator Contribution My collaborators have performed experiments to generate preliminary data for a collaborative grant application, contributed to the writing of the grant proposal and responded to reviewers comments.
Impact Submission of proposal entitled "Identification of conserved B-cell epitopes of highly pathogenic coronaviruses for broadspectrum immunotherapy and vaccine design" to the "One Health Approaches to Accelerate Vaccine Development" call that forms part of the UK government's commitment to Official Development Assistance (ODA) in October 2017.
Start Year 2017
 
Description Identification of conserved B-cell epitopes of highly pathogenic coronaviruses for broadspectrum immunotherapy and vaccine design. 
Organisation University of Kent
Country United Kingdom 
Sector Academic/University 
PI Contribution We have performed experiments to generate preliminary data for a collaborative grant application, contributed to the writing of the grant proposal and responded to reviewers comments.
Collaborator Contribution My collaborators have performed experiments to generate preliminary data for a collaborative grant application, contributed to the writing of the grant proposal and responded to reviewers comments.
Impact Submission of proposal entitled "Identification of conserved B-cell epitopes of highly pathogenic coronaviruses for broadspectrum immunotherapy and vaccine design" to the "One Health Approaches to Accelerate Vaccine Development" call that forms part of the UK government's commitment to Official Development Assistance (ODA) in October 2017.
Start Year 2017
 
Description Identification of genetic markers in the haemagglutinin glycoprotein critical for antigenic activity of H9N2 avian influenza viruses. 
Organisation Francis Crick Institute
Country United Kingdom 
Sector Academic/University 
PI Contribution d provision of reagents and virus samples.
Collaborator Contribution Technical training to PhD students and use of research facilities
Impact (1) Talk: Avian influenza virus evolution: immune escape, increase in zoonotic potential and fitness in poultry. Presented at "Prevention and Control Techniques for Infectious Diseases in Livestock and Poultry", 27th 29th August 2017, Beijing, China. (2) Impact of avian influenza virus evolution on antigenicity and zoonotic infection potential. Presented at University of Veterinary and Animal Sciences (UVAS) Lahore, Pakistan on 23rd August 2017. (3). Molecular determinants of H9N2 avian influenza virus influencing antigenicity and receptor binding. Presented at CERAD (Centre of Excellence for Research on Avian Diseases), 3rd Symposium Recent Advances in Avian Disease Research. 31st August - 1st September 2017 at the Harbour Hotel, Alexandra Terrace, Guildford, Surrey, UK. (4) Overview of ongoing research on Poultry diseases in United Kingdom. Presented as at International poultry Stakeholders work on poultry biosecurity in Sri Lanka from 26th February to 3rd March 2017. (5). Evolutionary fitness of avian influenza viruses and development of improved vaccines and diagnostics. Presented as invited seminar speaker at RVC (Hawkshead Campus), UK. 19th October 2016.
Start Year 2012
 
Description Identification of genetic markers in the haemagglutinin glycoprotein critical for antigenic activity of H9N2 avian influenza viruses. 
Organisation Imperial College London
Department Section of Virology
Country United Kingdom 
Sector Academic/University 
PI Contribution d provision of reagents and virus samples.
Collaborator Contribution Technical training to PhD students and use of research facilities
Impact (1) Talk: Avian influenza virus evolution: immune escape, increase in zoonotic potential and fitness in poultry. Presented at "Prevention and Control Techniques for Infectious Diseases in Livestock and Poultry", 27th 29th August 2017, Beijing, China. (2) Impact of avian influenza virus evolution on antigenicity and zoonotic infection potential. Presented at University of Veterinary and Animal Sciences (UVAS) Lahore, Pakistan on 23rd August 2017. (3). Molecular determinants of H9N2 avian influenza virus influencing antigenicity and receptor binding. Presented at CERAD (Centre of Excellence for Research on Avian Diseases), 3rd Symposium Recent Advances in Avian Disease Research. 31st August - 1st September 2017 at the Harbour Hotel, Alexandra Terrace, Guildford, Surrey, UK. (4) Overview of ongoing research on Poultry diseases in United Kingdom. Presented as at International poultry Stakeholders work on poultry biosecurity in Sri Lanka from 26th February to 3rd March 2017. (5). Evolutionary fitness of avian influenza viruses and development of improved vaccines and diagnostics. Presented as invited seminar speaker at RVC (Hawkshead Campus), UK. 19th October 2016.
Start Year 2012
 
Description Improving vaccines and diagnostics for avian influenza viruses 
Organisation Zagazig University
Country Egypt 
Sector Academic/University 
PI Contribution Provision of Research training, analysis of field samples
Collaborator Contribution Provision of Field data and samples
Impact Characterisation of Avian Influenza Virus Hemagglutinin from Egyptian Chicken Flocks during 2016 Outbreaks. Presented at Global Alliance for Research on Avian Diseases conference. Hanoi, VietNam17-19 January 2018.
Start Year 2017
 
Description Investigating antigenic determinants inducing stronger and broader cross-protective immunity among H5 avian influenza viruses 
Organisation Royal Veterinary College (RVC)
Department Veterinary Basic Sciences
Country United Kingdom 
Sector Academic/University 
PI Contribution The overarching aims of the proposed research are to improve controls against H5 subtype of avian influenza viruses infecting poultry by investigating underlying mechanisms that define how vaccines renders effectiveness and develop novel approaches enhancing the effectiveness of H5 avian influenza vaccines. We recently generated a panel of monoclonal antibodies (mAbs) that recognise a variety of different clades of H5 viruses. Propagation of AIV in eggs or cultured cells together with mAbs drives virus to escape from mAbs neutralisation activity. These classical virus neutralization assays (VN) followed by HA gene sequence analysis allowed us to defined the role of amino acid substitutions that are associated with evasion of antibody neutralization through emergence of antigenic variants and failure of vaccine efficacy.
Collaborator Contribution provided intellectual input for achieving prescribed objectives of this work.
Impact Talk entitled "Molecular determinants for antigenicity and vaccine efficacy of avian influenza viruses" was presented at Newton Agham Researcher Links Workshop " Novel Vaccines and Diagnostic Technologies against Emerging and re-emerging Veterinary Pathogens" at Rizal Park Hotel, Manila, Philippines, 4-7 February 2019.
Start Year 2019
 
Description Investigating antigenic determinants inducing stronger and broader cross-protective immunity among H5 avian influenza viruses. 
Organisation Royal Veterinary College (RVC)
Country United Kingdom 
Sector Academic/University 
PI Contribution We developed research programme to characterise diversity of antigenic epitopes induce protective immunity in chickens against H5 subtypes of avian influenza viruses.
Collaborator Contribution Collaborating partner Dr Nicola Lewis at Royal Veterinary College provide advise to on cartography programme to visualize the antigenic similarity or difference among different strains of influenza viruses.
Impact The work under this collaborative determined the antigenic differences among different clades of H5 avian influenza viruses circulating in birds. This project is multi-disciplinary. our laboratory mainly work on virology and immunology part of the project and we seek help on computer modeling part from the collaborating partner Dr Nicola Lewis.
Start Year 2019
 
Description Leeds FBS 
Organisation University of Leeds
Department Leeds Institute of Molecular Medicine
Country United Kingdom 
Sector Academic/University 
PI Contribution Research
Collaborator Contribution Research and student supervision
Impact Research
Start Year 2009
 
Description Martin Ludlow - University of Veterinary Medicine, Hanover. Morbillivirus collaboration. 
Organisation University of Veterinary Medicine Hanover
Country Germany 
Sector Academic/University 
PI Contribution Provision of technical information and advice about the host-range of morbilliviruses.
Collaborator Contribution Provision of reagents to support our research project, e.g. plasmid clones of viral attachment proteins and receptors.
Impact No outcomes as yet, only preliminary data.
Start Year 2018
 
Description Miguel Ángel Muñoz Alía - Mayo clinic, USA. Measles population diversity. 
Organisation Mayo Clinic
Country United States 
Sector Charity/Non Profit 
PI Contribution Technical support and sharing of research data on the host-range of morbilliviruses.
Collaborator Contribution Provision of plasmid clones representing the broad genetic diversity of measles attachment and fusion proteins. Technical support.
Impact No outcomes as yet.
Start Year 2018
 
Description Miles Carroll PHE collaboration on pseudotyping 
Organisation Public Health England
Country United Kingdom 
Sector Public 
PI Contribution Provision of reagents to perform pseudotyping of Ebola G protein
Collaborator Contribution Discussion of preliminary data
Impact None to date, publications in preparation.
Start Year 2019
 
Description Molecular epidemiology of H9N2 avian influenza viruses in Vietnam and Pakistan. 
Organisation Royal Veterinary College (RVC)
Country United Kingdom 
Sector Academic/University 
PI Contribution Exchange of technical ideas, reagents and samples.
Collaborator Contribution Provision of field samples and data on avian influenza infection in poultry. This diseases is endemic in poultry in Pakistan.
Impact (1) Increased receptor binding avidity of H9N2 avian influenza viruses is associated with escape from antibody based immunity and enhanced zoonotic potential. presented by Joshua Sealy at GARAD and ZELS conferences in Haneoi, Vietnam from 17-27 January 2018.
Start Year 2008
 
Description Molecular epidemiology of H9N2 avian influenza viruses in Vietnam and Pakistan. 
Organisation University of Veterinary & Animal Sciences
Department Department of Microbiology
Country Pakistan 
Sector Academic/University 
PI Contribution Exchange of technical ideas, reagents and samples.
Collaborator Contribution Provision of field samples and data on avian influenza infection in poultry. This diseases is endemic in poultry in Pakistan.
Impact (1) Increased receptor binding avidity of H9N2 avian influenza viruses is associated with escape from antibody based immunity and enhanced zoonotic potential. presented by Joshua Sealy at GARAD and ZELS conferences in Haneoi, Vietnam from 17-27 January 2018.
Start Year 2008
 
Description Morphological differences of Influenza A viruses 
Organisation University of Edinburgh
Department The Roslin Institute
Country United Kingdom 
Sector Academic/University 
PI Contribution Exchange of research methods and reagents
Collaborator Contribution (1) Exchange of reagents and Research methods.(2) training of PhD students and Postdoctoral Scientists (3) use of research facilities
Impact Research output were presented at Avian Influenza A virus budding morphology: spherical or filamentous? presented at The European Scientific Working group on Influenza (ESWI) Riga, Latvia. 10 to 13 September 2017 (2) .Budding morphology of avian influenza A viruses. Microbiology Society, annual Conference 2017, 306 April, EICC, Edinburggh, UK
Start Year 2016
 
Description PPRV collaboration with Catherine Herzog at Penn State 
Organisation Penn State University
Country United States 
Sector Academic/University 
PI Contribution Advice on design of animal experiments
Collaborator Contribution Leading project on examination of PPRV infection in cattle.
Impact No outputs to date
Start Year 2019
 
Description Partnering with National Taiwan University 
Organisation National Taiwan University
Country Taiwan, Province of China 
Sector Academic/University 
PI Contribution We have prepared and submitted an application for a BBSRC-Taiwan Partnering Award to study "Assembly of Recombinant Infectious Bronchitis Virus and Determination of Antigenic Sites that Confer Hemagglutination Activity".
Collaborator Contribution The partners conceived the project and got in contact about submitting an application for funding together.
Impact An application has been made for a BBSRC-Taiwan Partnering Award in November 2018.
Start Year 2018
 
Description Paul Digard & Reidun Twarock - Packaging signals 
Organisation University of Edinburgh
Department The Roslin Institute
Country United Kingdom 
Sector Academic/University 
PI Contribution Supply of reverse genetic avian influenza viruses for the successful award of a PhD studentship based at The Roslin Institute entitled "Identification of interaction sites between the genomic segments of influenza virus as putative novel anti-viral targets".
Collaborator Contribution University of York is providing mathematical modelling algorithms of viral packaging. Paul Digard- Roslin Institute is providing influenza virology expertise and hosting the student.
Impact Studentship commenced in October 2019
Start Year 2019
 
Description Paul Digard & Reidun Twarock - Packaging signals 
Organisation University of York
Country United Kingdom 
Sector Academic/University 
PI Contribution Supply of reverse genetic avian influenza viruses for the successful award of a PhD studentship based at The Roslin Institute entitled "Identification of interaction sites between the genomic segments of influenza virus as putative novel anti-viral targets".
Collaborator Contribution University of York is providing mathematical modelling algorithms of viral packaging. Paul Digard- Roslin Institute is providing influenza virology expertise and hosting the student.
Impact Studentship commenced in October 2019
Start Year 2019
 
Description Pseudotyping virus collaboration with Dr. Tess Lambe, University of Oxford 
Organisation University of Oxford
Country United Kingdom 
Sector Academic/University 
PI Contribution Development of assays and reagents to support successful pseudotyping of various viruses for quantitative entry assays
Collaborator Contribution Provision of reagents and plasmid clones
Impact No outputs yet,
Start Year 2019
 
Description Rapid acquisition of mammalian characteristics by avian influenza virus in single host infections. 
Organisation Imperial College London
Country United Kingdom 
Sector Academic/University 
PI Contribution Provided reagents and day to day technical help for undertaking this project and supervising postdoctoral researcher working on this project. Reagents include influenza reverse genetic plasmids and H9N2 and H7N9-specific antibodies.
Collaborator Contribution this project will aim to understand AIV genetics that facilitate the rapid acquisition of mammalian adaptation characteristics in a single host infection. The partners with our collaborations determined adaptive genetic changes in the H9N2 avian influenza virus following infection in mice (mammalian hosts). Different strains of avian influenza Viruses generated by reverse gentic techniques were inoculated into mice and lungs sampled daily. RNA recovered from lung homogenates were deep sequenced and mutations arising were characterised for adaptation. Relative viral fitness and the rapidity of the accumulation of mutations was measured and compared amongst the viral strains.
Impact The results of the our experimental studies showed the avian-origin viruses rapidly acquired mutations that increase virus fitness in mammalian species. Therefore, these viruses pose zoonotic and pandemic threat to public health.
Start Year 2018
 
Description Rapid acquisition of mammalian characteristics by avian influenza virus in single host infections. 
Organisation The Pirbright Institute
Country United Kingdom 
Sector Academic/University 
PI Contribution Provided reagents and day to day technical help for undertaking this project and supervising postdoctoral researcher working on this project. Reagents include influenza reverse genetic plasmids and H9N2 and H7N9-specific antibodies.
Collaborator Contribution this project will aim to understand AIV genetics that facilitate the rapid acquisition of mammalian adaptation characteristics in a single host infection. The partners with our collaborations determined adaptive genetic changes in the H9N2 avian influenza virus following infection in mice (mammalian hosts). Different strains of avian influenza Viruses generated by reverse gentic techniques were inoculated into mice and lungs sampled daily. RNA recovered from lung homogenates were deep sequenced and mutations arising were characterised for adaptation. Relative viral fitness and the rapidity of the accumulation of mutations was measured and compared amongst the viral strains.
Impact The results of the our experimental studies showed the avian-origin viruses rapidly acquired mutations that increase virus fitness in mammalian species. Therefore, these viruses pose zoonotic and pandemic threat to public health.
Start Year 2018
 
Description Stephen Graham collaboration on structural biology with University of Cambridge 
Organisation University of Cambridge
Country United Kingdom 
Sector Academic/University 
PI Contribution Provision of data and sharing of experimental ideas regarding viral host range and techniques for simulating virus evolution
Collaborator Contribution Structural analysis of mutants to viral glycoproteins
Impact Journal of Virology paper on potential PPRV emergence in human populations (Abdullah et al, 2018), other papers in preparation.
Start Year 2018
 
Description The GCRF One Health Poultry Hub 
Organisation Royal Veterinary College (RVC)
Country United Kingdom 
Sector Academic/University 
PI Contribution The focus of the research is to achieve sustainable global intensification of poultry meat and egg production whilst reducing risks to human and animal health and welfare. We are undertaking research to investigate the diversity of avian influenza viruses prevalent in poultry and wild birds in south east Asia.
Collaborator Contribution Royal Veterinary College, London is leading this GCRF One Health Poultry Hub. The project will address the need to meet rising demand for poultry meat and eggs in developing countries, while minimising risk to international public health. Population growth is driving global demand for poultry, meat and egg production; this unfortunately creates conditions in which animal diseases can spread to humans ('zoonoses'). These include bacterial food poisoning and strains with avian influenza with epidemic or pandemic potential. The GCRF One Health Poultry Hub will adopt a 'One Health' approach to the issue of combatting animal-to-human diseases by bringing together a team of laboratory, clinical, veterinary and social scientists. This team will test and evaluate novel interventions. The need for safe poultry production is most urgent in South and South East Asia, so the RVC and its partners will then use their local networks in these regions to put its positive research to immediate use.
Impact Development of international interdisciplinary partnership of 55 investigators from 13 countries to address the emerging global challenges of food security and public health associated with intensification of poultry production.
Start Year 2018
 
Description The Pirbright "Livestock Antibody Hub" funded by Bill & Melinda Gates Foundation 
Organisation The Pirbright Institute
Country United Kingdom 
Sector Academic/University 
PI Contribution Me and my team established collaboration in the "Livestock Antibody Hub" funded ($5.5 million) by the from Bill & Melinda Gates Foundation. Focus of our partnership is to develop techniques and capacity to analyse immune cells (B cells) of cattle, poultry and pigs that produce virus neutralizing antibodies. This research will support rational development of next generation of vaccines and to explore antibody-based passive immunization approaches for treatment and prophylaxis of infectious viral diseases affecting livestock and humans. Our initial focus is to target major animal viral pathogens that cause severe losses within Low and Middle-Income Countries (LMIC) animal production systems including avian influenza virus (AIV), Newcastle disease virus (NDV), infectious bursal disease virus (IBDV), infectious bronchitis virus (IBV) and foot-and-mouth disease virus (FMDV).
Collaborator Contribution This is a collaborative project and partners are providing help in provision of reagents and techniques for analysis of diversity of B cells repertoire that produce infleunza virus-specific antibodies, single cell sequencing and bioinformatic analysis. This project will further strengthen our on-going research collaborations with many research groups in academia as well as with animal health industry (Zoetis, Boehringer Ingelheim Animal Health, Merck, CEVA, Jovac, YeBio, ILRI, Galvmed, The Roslin Institute, HuMabs, AbCellera and Distributed Bio) to take the research outputs from laboratory to the field. The outputs of research will be improved vaccines and diagnostics enabling to reduce the impact of infectious diseases on farm animals, which offer substantial direct and indirect economic, public health, environmental and social benefits to the UK and rest of the world.
Impact Development of passive immunization approaches against avian influenza viruses affecting poultry (project incited in January 2020).
Start Year 2020
 
Description The role of segment 3 in H9N2 avian influenza virus pathogenicity 
Organisation University of Edinburgh
Department The Roslin Institute
Country United Kingdom 
Sector Academic/University 
PI Contribution This is a Joint studentship project funded by The Pirbright Institute and The Roslin Institute. We identiifed molecular markers that are responsible for increase in virus pathogenicity and transmission.
Collaborator Contribution The Roslin Institute contributed both intellectually and by proving research training, samples and regents to achieve the prescribed objectives this project.
Impact 1. Oral Presentation Title: Identification of segment 3 sequence polymorphisms which alter shut off activity and pathogenicity of H9N2 avian influenza viruses in vivo. Anabel Clements, Saira Hussain, Holly Shelton, Paul Digard and Munir Iqbal. Global Alliance for Research Into Avian Diseases (GARAD). January 17th to 19th 2018. Fortuna Hotel, Lang Ha Street, Hanoi, VietNam 2. Poster title: What is the role of H9N2 avian influenza virus PA-X within an avian model?. Anabel Clements, Saira Hussain, Holly Shelton, Paul Digard & Munir Iqbal. UK- China CERAD (Centre of Excellence for Research on Avian Diseases), 3rd Symposium Recent Advances in Avian Disease Research. August 31st - 1st September 2017. The Harbour Hotel, Alexandra Terrace, Guildford, Surrey, UK. 3. Poster title: What is the role of H9N2 avian influenza virus PA-X within an avian model?. Anabel Clements, Saira Hussain, Holly Shelton, Paul Digard & Munir Iqbal. Microbiology Society Annual Conference. April 3rd-6th 2017. Edinburgh International Conference Centre (EICC), Edinburgh, UK. 4. Oral Presentation title: Identification of sequence polymorphisms in segment 3 of H9N2 avian influenza viruses that affect PA-X activity. Anabel Clements, Saira Hussain, Holly Shelton, Paul Digard & Munir Iqbal. Focused Meeting 2016: Molecular Biology and Pathogenesis of Avian Viruses. September 27th-29th 2016. Charles Darwin House, London, UK. 5. Oral Presentation title: The role of segment 3 in H9N2 avian influenza virus pathogenicity. Anabel Clements, Saira Hussain, Holly Shelton, Munir Iqbal & Paul Digard. UK -Taiwan BBSRC Partnering Award workshop: Improving Control of influenza viruses. 26th-27th April 2016. Taipei, Taiwan. 6. Oral Presentation title: Identification of sequence polymorphisms in segment 3 of H9N2 avian influenza viruses that affect PA-X activity. Anabel Clements, Saira Hussain, Holly Shelton, Munir Iqbal & Paul Digard. Microbiology Society Annual Conference 2016; virus Workshop: Negative strand RNA Viruses. 21st-24th March 2016. Arena and Convention Centre, Liverpool, UK. 7. Poster title: The role of segment 3 in H9N2 influenza virus pathogenicity. Anabel Clements, Saira Hussain, Holly Shelton, Munir Iqbal & Paul Digard. 7th Orthomyxovirus research conference. 16th-18th September 2015. Ecole Nationale Veterinaire de Toulouse, Toulouse, France. 8. Poster title: The role of segment 3 in H9N2 influenza virus pathogenicity. Anabel Clements, Saira Hussain, Holly Shelton, Munir Iqbal & Paul Digard. Edinburgh Infectious Diseases Annual Symposium. 20th May 2015. Royal College of Physicians, Edinburgh, UK.
Start Year 2014
 
Description Thomas Bowden - STRUBI - University of Oxford - Glycoprotein structure collaboration 
Organisation University of Oxford
Country United Kingdom 
Sector Academic/University 
PI Contribution Provision of data and advice about the biological properties of viral glycoproteins, e.g. their fusion capacity with various receptors, their role in determining host-range
Collaborator Contribution Advice and technical support at the structural level supporting future project development and design
Impact No outcomes as yet
Start Year 2018
 
Description Understanding antigenic diversity, zoonotic potential and immunological prevention of avian influenza viruses affecting poultry 
Organisation Beijing Academy of Agriculture and Forestry Sciences
Country China 
Sector Academic/University 
PI Contribution Undertake joint research work to investigate to identify the molecular determinants that are critical for enhancing potency and efficacy of vaccines, as well as those that are associated with heightened zoonotic risk.
Collaborator Contribution Provision of samples, reagents and research facilities
Impact Published joint paper (1)ian Xu, Xixi Zhang, Shuanghai Zhou, Junjun Shen,Dawei Yang, Jing Wu, Xiaoyang Li, Meiling Li, Xiufen Huang, Joshua E. Sealy, Munir Iqbal&Yongqing Li (2017). A DNA aptamer efficiently inhibits the infectivity of Bovine herpesvirus 1 by blocking viral entry. Scientific Reports. 7: 11796. doi:10.1038/s41598-017-10070-1. (2) Jian Xu, Jing Wu, Houjun He, Junjun Shena, Dawei Yang, Xixi Zhang, Xiaoyang Li, Meiling Li, Xiufen Huang, Joshua Sealy, Munir Iqbal & Yongqing Li (2017). Bovine single chain Fv antibody inhibits bovine herpesvirus-1 infectivity by targeting viral glycoprotein D. Applied Microbiology and Biotechnology. https://doi.org/10.1007/s00253-017-8566-0. (2)
Start Year 2017
 
Description Understanding antigenic diversity, zoonotic potential and immunological prevention of avian influenza viruses affecting poultry 
Organisation China Agricultural University (CAU)
Department College of Veterinary Medicine
Country China 
Sector Academic/University 
PI Contribution Undertake joint research work to investigate to identify the molecular determinants that are critical for enhancing potency and efficacy of vaccines, as well as those that are associated with heightened zoonotic risk.
Collaborator Contribution Provision of samples, reagents and research facilities
Impact Published joint paper (1)ian Xu, Xixi Zhang, Shuanghai Zhou, Junjun Shen,Dawei Yang, Jing Wu, Xiaoyang Li, Meiling Li, Xiufen Huang, Joshua E. Sealy, Munir Iqbal&Yongqing Li (2017). A DNA aptamer efficiently inhibits the infectivity of Bovine herpesvirus 1 by blocking viral entry. Scientific Reports. 7: 11796. doi:10.1038/s41598-017-10070-1. (2) Jian Xu, Jing Wu, Houjun He, Junjun Shena, Dawei Yang, Xixi Zhang, Xiaoyang Li, Meiling Li, Xiufen Huang, Joshua Sealy, Munir Iqbal & Yongqing Li (2017). Bovine single chain Fv antibody inhibits bovine herpesvirus-1 infectivity by targeting viral glycoprotein D. Applied Microbiology and Biotechnology. https://doi.org/10.1007/s00253-017-8566-0. (2)
Start Year 2017
 
Description Understanding antigenic diversity, zoonotic potential and immunological prevention of avian influenza viruses affecting poultry 
Organisation Chinese Center for Disease Control and Prevention (China CDC)
Country China 
Sector Public 
PI Contribution Undertake joint research work to investigate to identify the molecular determinants that are critical for enhancing potency and efficacy of vaccines, as well as those that are associated with heightened zoonotic risk.
Collaborator Contribution Provision of samples, reagents and research facilities
Impact Published joint paper (1)ian Xu, Xixi Zhang, Shuanghai Zhou, Junjun Shen,Dawei Yang, Jing Wu, Xiaoyang Li, Meiling Li, Xiufen Huang, Joshua E. Sealy, Munir Iqbal&Yongqing Li (2017). A DNA aptamer efficiently inhibits the infectivity of Bovine herpesvirus 1 by blocking viral entry. Scientific Reports. 7: 11796. doi:10.1038/s41598-017-10070-1. (2) Jian Xu, Jing Wu, Houjun He, Junjun Shena, Dawei Yang, Xixi Zhang, Xiaoyang Li, Meiling Li, Xiufen Huang, Joshua Sealy, Munir Iqbal & Yongqing Li (2017). Bovine single chain Fv antibody inhibits bovine herpesvirus-1 infectivity by targeting viral glycoprotein D. Applied Microbiology and Biotechnology. https://doi.org/10.1007/s00253-017-8566-0. (2)
Start Year 2017
 
Description Understanding antigenic diversity, zoonotic potential and immunological prevention of avian influenza viruses affecting poultry 
Organisation Francis Crick Institute
Country United Kingdom 
Sector Academic/University 
PI Contribution Undertake joint research work to investigate to identify the molecular determinants that are critical for enhancing potency and efficacy of vaccines, as well as those that are associated with heightened zoonotic risk.
Collaborator Contribution Provision of samples, reagents and research facilities
Impact Published joint paper (1)ian Xu, Xixi Zhang, Shuanghai Zhou, Junjun Shen,Dawei Yang, Jing Wu, Xiaoyang Li, Meiling Li, Xiufen Huang, Joshua E. Sealy, Munir Iqbal&Yongqing Li (2017). A DNA aptamer efficiently inhibits the infectivity of Bovine herpesvirus 1 by blocking viral entry. Scientific Reports. 7: 11796. doi:10.1038/s41598-017-10070-1. (2) Jian Xu, Jing Wu, Houjun He, Junjun Shena, Dawei Yang, Xixi Zhang, Xiaoyang Li, Meiling Li, Xiufen Huang, Joshua Sealy, Munir Iqbal & Yongqing Li (2017). Bovine single chain Fv antibody inhibits bovine herpesvirus-1 infectivity by targeting viral glycoprotein D. Applied Microbiology and Biotechnology. https://doi.org/10.1007/s00253-017-8566-0. (2)
Start Year 2017
 
Description Understanding antigenic diversity, zoonotic potential and immunological prevention of avian influenza viruses affecting poultry 
Organisation University of Glasgow
Department Institute of Biodiversity, Animal Health and Comparative Medicine
Country United Kingdom 
Sector Academic/University 
PI Contribution Undertake joint research work to investigate to identify the molecular determinants that are critical for enhancing potency and efficacy of vaccines, as well as those that are associated with heightened zoonotic risk.
Collaborator Contribution Provision of samples, reagents and research facilities
Impact Published joint paper (1)ian Xu, Xixi Zhang, Shuanghai Zhou, Junjun Shen,Dawei Yang, Jing Wu, Xiaoyang Li, Meiling Li, Xiufen Huang, Joshua E. Sealy, Munir Iqbal&Yongqing Li (2017). A DNA aptamer efficiently inhibits the infectivity of Bovine herpesvirus 1 by blocking viral entry. Scientific Reports. 7: 11796. doi:10.1038/s41598-017-10070-1. (2) Jian Xu, Jing Wu, Houjun He, Junjun Shena, Dawei Yang, Xixi Zhang, Xiaoyang Li, Meiling Li, Xiufen Huang, Joshua Sealy, Munir Iqbal & Yongqing Li (2017). Bovine single chain Fv antibody inhibits bovine herpesvirus-1 infectivity by targeting viral glycoprotein D. Applied Microbiology and Biotechnology. https://doi.org/10.1007/s00253-017-8566-0. (2)
Start Year 2017
 
Description Understanding diversity of avian influenza viruses and improvement disease control in poultry and humans 
Organisation Agricultural Research Centre
Department Veterinary Serum and Vaccine Research Institute
Country Egypt 
Sector Public 
PI Contribution This project helped my research group to establish research collboration between the Pirbright Institute and the scientists at the collaborating laboratories listed above and an excellent opportunity for building a joint "ONE HEALTH" platfor. I am sharing knowledge, reagents and techniques to improve efficacious vaccines and diagnostic tools against avian influenza viruses. The easy availability of better control tools against avian influenza viruses will aid in the reduction of poultry production losses and thus be important for global food security and improved animal welfare whilst also reducing zoonotic transmission to humans.
Collaborator Contribution Each collaborating institute and team member has specific expertise and facilities in areas relevant to this research programme. Collaborators in different institutions have extensive experience collecting field samples and implementing vaccination and large scale surveillance programmes. Each partner institutions have unique experience in monitoring the endemic prevalence of many animal diseases including avian influenza in both domestic and wild birds from which UK is under constant threat. The partners are sharing knowledge, expertise and facilities to investigate fundamental biology of influenza viruses and predict their epidemiological relevance, to assess potential risks and to devise appropriate intervention strategies. The knowledge, reagents and field samples will be available through this partnership. which will be essential for development of informed control policies and tools(vaccines and diagnostics) that may contribute to reducing the ever increasing threat from endemic, emerging and re-emerging influenza virus variants posing risk to animals and humans. Also, All researchers involved will benefit through ongoing professional development and advanced training in new technologies and new systems-level approaches to disease management. Exchange visits of researchers from collaborating laboratories to undertake training in a number of disciplines including disease surveillance, molecular virology, epidemiology, immunology, vaccinology and diagnostics will have a tangible impact on capacity building and training of next generation researchers in control of infectious animal and zoonotic diseases.
Impact The ultimate aims of this collaboration are to reduce the impact of influenza virus on poultry production and reduce their zoonotic transmission to humans. The collaboration will improve resources and capacity for disease control systems. Achieving these specific goals will prove a step-change in AIV disease management and increase in poultry productivity directly drives economic prosperity of farmers and allied communities. The will directly impact on socio-economic well-being of partner countries.
Start Year 2017
 
Description Understanding diversity of avian influenza viruses and improvement disease control in poultry and humans 
Organisation Francis Crick Institute
Country United Kingdom 
Sector Academic/University 
PI Contribution This project helped my research group to establish research collboration between the Pirbright Institute and the scientists at the collaborating laboratories listed above and an excellent opportunity for building a joint "ONE HEALTH" platfor. I am sharing knowledge, reagents and techniques to improve efficacious vaccines and diagnostic tools against avian influenza viruses. The easy availability of better control tools against avian influenza viruses will aid in the reduction of poultry production losses and thus be important for global food security and improved animal welfare whilst also reducing zoonotic transmission to humans.
Collaborator Contribution Each collaborating institute and team member has specific expertise and facilities in areas relevant to this research programme. Collaborators in different institutions have extensive experience collecting field samples and implementing vaccination and large scale surveillance programmes. Each partner institutions have unique experience in monitoring the endemic prevalence of many animal diseases including avian influenza in both domestic and wild birds from which UK is under constant threat. The partners are sharing knowledge, expertise and facilities to investigate fundamental biology of influenza viruses and predict their epidemiological relevance, to assess potential risks and to devise appropriate intervention strategies. The knowledge, reagents and field samples will be available through this partnership. which will be essential for development of informed control policies and tools(vaccines and diagnostics) that may contribute to reducing the ever increasing threat from endemic, emerging and re-emerging influenza virus variants posing risk to animals and humans. Also, All researchers involved will benefit through ongoing professional development and advanced training in new technologies and new systems-level approaches to disease management. Exchange visits of researchers from collaborating laboratories to undertake training in a number of disciplines including disease surveillance, molecular virology, epidemiology, immunology, vaccinology and diagnostics will have a tangible impact on capacity building and training of next generation researchers in control of infectious animal and zoonotic diseases.
Impact The ultimate aims of this collaboration are to reduce the impact of influenza virus on poultry production and reduce their zoonotic transmission to humans. The collaboration will improve resources and capacity for disease control systems. Achieving these specific goals will prove a step-change in AIV disease management and increase in poultry productivity directly drives economic prosperity of farmers and allied communities. The will directly impact on socio-economic well-being of partner countries.
Start Year 2017
 
Description Understanding diversity of avian influenza viruses and improvement disease control in poultry and humans 
Organisation Imperial College London
Department MSk Lab
Country United Kingdom 
Sector Academic/University 
PI Contribution This project helped my research group to establish research collboration between the Pirbright Institute and the scientists at the collaborating laboratories listed above and an excellent opportunity for building a joint "ONE HEALTH" platfor. I am sharing knowledge, reagents and techniques to improve efficacious vaccines and diagnostic tools against avian influenza viruses. The easy availability of better control tools against avian influenza viruses will aid in the reduction of poultry production losses and thus be important for global food security and improved animal welfare whilst also reducing zoonotic transmission to humans.
Collaborator Contribution Each collaborating institute and team member has specific expertise and facilities in areas relevant to this research programme. Collaborators in different institutions have extensive experience collecting field samples and implementing vaccination and large scale surveillance programmes. Each partner institutions have unique experience in monitoring the endemic prevalence of many animal diseases including avian influenza in both domestic and wild birds from which UK is under constant threat. The partners are sharing knowledge, expertise and facilities to investigate fundamental biology of influenza viruses and predict their epidemiological relevance, to assess potential risks and to devise appropriate intervention strategies. The knowledge, reagents and field samples will be available through this partnership. which will be essential for development of informed control policies and tools(vaccines and diagnostics) that may contribute to reducing the ever increasing threat from endemic, emerging and re-emerging influenza virus variants posing risk to animals and humans. Also, All researchers involved will benefit through ongoing professional development and advanced training in new technologies and new systems-level approaches to disease management. Exchange visits of researchers from collaborating laboratories to undertake training in a number of disciplines including disease surveillance, molecular virology, epidemiology, immunology, vaccinology and diagnostics will have a tangible impact on capacity building and training of next generation researchers in control of infectious animal and zoonotic diseases.
Impact The ultimate aims of this collaboration are to reduce the impact of influenza virus on poultry production and reduce their zoonotic transmission to humans. The collaboration will improve resources and capacity for disease control systems. Achieving these specific goals will prove a step-change in AIV disease management and increase in poultry productivity directly drives economic prosperity of farmers and allied communities. The will directly impact on socio-economic well-being of partner countries.
Start Year 2017
 
Description Understanding diversity of avian influenza viruses and improvement disease control in poultry and humans 
Organisation Imperial College London
Country United Kingdom 
Sector Academic/University 
PI Contribution This project helped my research group to establish research collboration between the Pirbright Institute and the scientists at the collaborating laboratories listed above and an excellent opportunity for building a joint "ONE HEALTH" platfor. I am sharing knowledge, reagents and techniques to improve efficacious vaccines and diagnostic tools against avian influenza viruses. The easy availability of better control tools against avian influenza viruses will aid in the reduction of poultry production losses and thus be important for global food security and improved animal welfare whilst also reducing zoonotic transmission to humans.
Collaborator Contribution Each collaborating institute and team member has specific expertise and facilities in areas relevant to this research programme. Collaborators in different institutions have extensive experience collecting field samples and implementing vaccination and large scale surveillance programmes. Each partner institutions have unique experience in monitoring the endemic prevalence of many animal diseases including avian influenza in both domestic and wild birds from which UK is under constant threat. The partners are sharing knowledge, expertise and facilities to investigate fundamental biology of influenza viruses and predict their epidemiological relevance, to assess potential risks and to devise appropriate intervention strategies. The knowledge, reagents and field samples will be available through this partnership. which will be essential for development of informed control policies and tools(vaccines and diagnostics) that may contribute to reducing the ever increasing threat from endemic, emerging and re-emerging influenza virus variants posing risk to animals and humans. Also, All researchers involved will benefit through ongoing professional development and advanced training in new technologies and new systems-level approaches to disease management. Exchange visits of researchers from collaborating laboratories to undertake training in a number of disciplines including disease surveillance, molecular virology, epidemiology, immunology, vaccinology and diagnostics will have a tangible impact on capacity building and training of next generation researchers in control of infectious animal and zoonotic diseases.
Impact The ultimate aims of this collaboration are to reduce the impact of influenza virus on poultry production and reduce their zoonotic transmission to humans. The collaboration will improve resources and capacity for disease control systems. Achieving these specific goals will prove a step-change in AIV disease management and increase in poultry productivity directly drives economic prosperity of farmers and allied communities. The will directly impact on socio-economic well-being of partner countries.
Start Year 2017
 
Description Understanding diversity of avian influenza viruses and improvement disease control in poultry and humans 
Organisation Suez Canal University
Country Egypt 
Sector Academic/University 
PI Contribution This project helped my research group to establish research collboration between the Pirbright Institute and the scientists at the collaborating laboratories listed above and an excellent opportunity for building a joint "ONE HEALTH" platfor. I am sharing knowledge, reagents and techniques to improve efficacious vaccines and diagnostic tools against avian influenza viruses. The easy availability of better control tools against avian influenza viruses will aid in the reduction of poultry production losses and thus be important for global food security and improved animal welfare whilst also reducing zoonotic transmission to humans.
Collaborator Contribution Each collaborating institute and team member has specific expertise and facilities in areas relevant to this research programme. Collaborators in different institutions have extensive experience collecting field samples and implementing vaccination and large scale surveillance programmes. Each partner institutions have unique experience in monitoring the endemic prevalence of many animal diseases including avian influenza in both domestic and wild birds from which UK is under constant threat. The partners are sharing knowledge, expertise and facilities to investigate fundamental biology of influenza viruses and predict their epidemiological relevance, to assess potential risks and to devise appropriate intervention strategies. The knowledge, reagents and field samples will be available through this partnership. which will be essential for development of informed control policies and tools(vaccines and diagnostics) that may contribute to reducing the ever increasing threat from endemic, emerging and re-emerging influenza virus variants posing risk to animals and humans. Also, All researchers involved will benefit through ongoing professional development and advanced training in new technologies and new systems-level approaches to disease management. Exchange visits of researchers from collaborating laboratories to undertake training in a number of disciplines including disease surveillance, molecular virology, epidemiology, immunology, vaccinology and diagnostics will have a tangible impact on capacity building and training of next generation researchers in control of infectious animal and zoonotic diseases.
Impact The ultimate aims of this collaboration are to reduce the impact of influenza virus on poultry production and reduce their zoonotic transmission to humans. The collaboration will improve resources and capacity for disease control systems. Achieving these specific goals will prove a step-change in AIV disease management and increase in poultry productivity directly drives economic prosperity of farmers and allied communities. The will directly impact on socio-economic well-being of partner countries.
Start Year 2017
 
Description Understanding diversity of avian influenza viruses and improvement disease control in poultry and humans 
Organisation University of Tokyo
Department International Research Center for Infectious Diseases
Country Japan 
Sector Hospitals 
PI Contribution This project helped my research group to establish research collboration between the Pirbright Institute and the scientists at the collaborating laboratories listed above and an excellent opportunity for building a joint "ONE HEALTH" platfor. I am sharing knowledge, reagents and techniques to improve efficacious vaccines and diagnostic tools against avian influenza viruses. The easy availability of better control tools against avian influenza viruses will aid in the reduction of poultry production losses and thus be important for global food security and improved animal welfare whilst also reducing zoonotic transmission to humans.
Collaborator Contribution Each collaborating institute and team member has specific expertise and facilities in areas relevant to this research programme. Collaborators in different institutions have extensive experience collecting field samples and implementing vaccination and large scale surveillance programmes. Each partner institutions have unique experience in monitoring the endemic prevalence of many animal diseases including avian influenza in both domestic and wild birds from which UK is under constant threat. The partners are sharing knowledge, expertise and facilities to investigate fundamental biology of influenza viruses and predict their epidemiological relevance, to assess potential risks and to devise appropriate intervention strategies. The knowledge, reagents and field samples will be available through this partnership. which will be essential for development of informed control policies and tools(vaccines and diagnostics) that may contribute to reducing the ever increasing threat from endemic, emerging and re-emerging influenza virus variants posing risk to animals and humans. Also, All researchers involved will benefit through ongoing professional development and advanced training in new technologies and new systems-level approaches to disease management. Exchange visits of researchers from collaborating laboratories to undertake training in a number of disciplines including disease surveillance, molecular virology, epidemiology, immunology, vaccinology and diagnostics will have a tangible impact on capacity building and training of next generation researchers in control of infectious animal and zoonotic diseases.
Impact The ultimate aims of this collaboration are to reduce the impact of influenza virus on poultry production and reduce their zoonotic transmission to humans. The collaboration will improve resources and capacity for disease control systems. Achieving these specific goals will prove a step-change in AIV disease management and increase in poultry productivity directly drives economic prosperity of farmers and allied communities. The will directly impact on socio-economic well-being of partner countries.
Start Year 2017
 
Description Understanding diversity of avian influenza viruses and improvement disease control in poultry and humans 
Organisation Zagazig University
Country Egypt 
Sector Academic/University 
PI Contribution This project helped my research group to establish research collboration between the Pirbright Institute and the scientists at the collaborating laboratories listed above and an excellent opportunity for building a joint "ONE HEALTH" platfor. I am sharing knowledge, reagents and techniques to improve efficacious vaccines and diagnostic tools against avian influenza viruses. The easy availability of better control tools against avian influenza viruses will aid in the reduction of poultry production losses and thus be important for global food security and improved animal welfare whilst also reducing zoonotic transmission to humans.
Collaborator Contribution Each collaborating institute and team member has specific expertise and facilities in areas relevant to this research programme. Collaborators in different institutions have extensive experience collecting field samples and implementing vaccination and large scale surveillance programmes. Each partner institutions have unique experience in monitoring the endemic prevalence of many animal diseases including avian influenza in both domestic and wild birds from which UK is under constant threat. The partners are sharing knowledge, expertise and facilities to investigate fundamental biology of influenza viruses and predict their epidemiological relevance, to assess potential risks and to devise appropriate intervention strategies. The knowledge, reagents and field samples will be available through this partnership. which will be essential for development of informed control policies and tools(vaccines and diagnostics) that may contribute to reducing the ever increasing threat from endemic, emerging and re-emerging influenza virus variants posing risk to animals and humans. Also, All researchers involved will benefit through ongoing professional development and advanced training in new technologies and new systems-level approaches to disease management. Exchange visits of researchers from collaborating laboratories to undertake training in a number of disciplines including disease surveillance, molecular virology, epidemiology, immunology, vaccinology and diagnostics will have a tangible impact on capacity building and training of next generation researchers in control of infectious animal and zoonotic diseases.
Impact The ultimate aims of this collaboration are to reduce the impact of influenza virus on poultry production and reduce their zoonotic transmission to humans. The collaboration will improve resources and capacity for disease control systems. Achieving these specific goals will prove a step-change in AIV disease management and increase in poultry productivity directly drives economic prosperity of farmers and allied communities. The will directly impact on socio-economic well-being of partner countries.
Start Year 2017
 
Description York 
Organisation University of York
Country United Kingdom 
Sector Academic/University 
PI Contribution Collaboration
Collaborator Contribution Collaboration
Impact Collaboration
Start Year 2015
 
Description Talk Presented at 2nd International Congress of Veterinary Microbiology, 16th -19th October 2018. Sherwood Breezes Resort-Lara, Antalya, Turkey. 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Conference discussed improving prevention and control of viral diseases of poultry.
Year(s) Of Engagement Activity 2018
 
Description "Challenges for Poultry Industry". Organised by Fakieh Poultry at the Ritz-Carlton Hotel in Jeddah, Saudi Arabia, 2nd -3rd December 2018, 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Conference discussed prevention and control of infectious diseases affecting poultry production.
Year(s) Of Engagement Activity 2018
 
Description "Interactive session with poultry stakeholders". Title: Overview of Avian Influenza group Research at The Pirbright Institute. Presented at University of Veterinary and Animal Sciences (UVAS) Lahore, Pakistan, 3rd August 2018. 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact The workshop discussed the economic impacts and prevention strategies against avian influenza viruses affecting poultry production and zoonotic infections.
Year(s) Of Engagement Activity 2018
 
Description 1st JSPS Core-to-Core Program Joint Seminar and the 7th CADIC International Symposium "Livestock Revolution in ASEAN for the Kitchen of the World" 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Don King provided an invited Keynote presentation to 1st JSPS Core-to-Core Program Joint Seminar and the 7th CADIC International Symposium "Livestock Revolution in ASEAN for the Kitchen of the World", Bangkok, Thailand, October 2017 on work undertaken at Pirbright to monitor the evolution of FMDV and threats posed for transbounadary spread.
Year(s) Of Engagement Activity 2017
 
Description 2. Overview of Avian Influenza group Research at The Pirbright Institute. Presented at "Interactive session with poultry stakeholders" at University of Veterinary and Animal Sciences (UVAS) Lahore, Pakistan, 3rd August 2018. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Dissemination of research Project outcomes These include presenting research data for vaccine seed strains with emergent field avian influenza variants; The zoonotic risk posed by emerging avian influenza viruses circulating in the wild birds and poultry. Impact of virus evolution on vaccine efficacy and persistence in poultry.
Year(s) Of Engagement Activity 2018
 
Description 33rd World Veterinary Congress, Incheon, South Korea, August 2017 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Don King was an invited speaker at the 33rd World Veterinary Congress, Incheon, South Korea, August 2017. He presented a talk entitled: New tools for the detection and characterisation of transboundary diseases.
Year(s) Of Engagement Activity 2017
URL http://wvc2017korea.com/
 
Description BBSRC Partnering Seminar, University of Liverpool, Tamilnadu Veterinary and Animal Science University 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Talk presented entitled " Molecular determinants of antigenicity of H7 and H9 avian Influenza viruses". The research data shared with the colleagues working to reduce the impact of avian infectious diseases on poultry production. The discussion led to more collaboration and work together, acquiring reagents and protocols for further research and development activities.
Year(s) Of Engagement Activity 2019
 
Description Blenheim High School Fair - DB 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Team members attended sessions at a local high school with secondary school children and those in the 6th form to talk about paths into scientific careers, sharing their experiences of working at The Pirbright Institute. Approximately 300 students attended and it stimulated increased interest in science and research.
Year(s) Of Engagement Activity 2019
 
Description Centre of excellence for research on avian diseases (CERAD) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact I was invited to present my work at the UK-China Centre of excellence for research on avian diseases (CERAD) meeting attended by researchers from UK, China and Thailand. There was lots of discussion about the research and future directions, including potential collaborations.
Year(s) Of Engagement Activity 2017
 
Description Cheltenham Science Festival 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Panel discussion at the Cheltenham Science Festival in 2018
One of the biggest threats to our food security is disease of our livestock and crops. Disease affects our economy and the availability of food in our shops. Don King, Head of the World Reference Lab for Foot-and-Mouth Disease, chemical ecologist John Pickett and food systems researcher John Ingram join climate scientist Tamsin Edwards to explore the risks to the UK's food supply and how preventing and controlling these diseases keeps our store cupboards well-stocked.
Year(s) Of Engagement Activity 2018
URL https://www.cheltenhamfestivals.com/science/whats-on/2018/how-safe-is-our-food-supply/
 
Description Cheltenham Science festival - LB 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact National science festival which Team members attended to demonstrate to the public how scientists at The Pirbright Institute can work to contain and control viral outbreaks. Over 500 people accessed the information and this stimulated increased interest in science and research.
Year(s) Of Engagement Activity 2019
 
Description Combating avian influenza through systematic analysis of antigenic drift, genetic variation, and development of novel diagnostic tools and vaccines. Presented at as PI of the ZELS project at ZELS grant holders and Stakeholders meeting in Hanoi, Vietnam 23th-24th January 2017. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Engagement and dissemination of research outcome to representatives of research funding bodies such as Department for International Development (DFID), Medical Research Council (MRC) and the researchers working on different research projects funded by BBSRC under Zoonoses and Emerging Livestock Systems (ZELS).
Year(s) Of Engagement Activity 2018
 
Description Conor Haydon participated in the STEM Day - Speed interviews and Careers Day 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Schools
Results and Impact STEM day engagement and careers
Year(s) Of Engagement Activity 2018
 
Description Deimante Lukosaityte: Microbiology Society conference 2019. Poster presentation: Chicken protection against H9N2 virus by passive immunization 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Described techniques for development of next generation of vaccines against viral diseases affecting animals and humans.
Year(s) Of Engagement Activity 2019
 
Description Demonstrating Mathematics (Angita Shrestha) 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Postgraduate students
Results and Impact Angita Shrestha (PhD student within Avian Influenza group) provided help in a Maths session to the new Oxford Doctoral Training Porgramme (DTP) cohort.
Year(s) Of Engagement Activity 2018
 
Description Designed a poster for presentation at the EuFMD conference - Title: Using high throughput sequencing to characterise low-frequency diversity of foot-and-mouth disease virus during vaccine strain adaptation 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact I prepared a poster presentation for the EuFMDV conference (Presentated by Boehringer Ingelheim) in regards to the research activity between the Pirbright Institute and Boehringer Ingelheim.
Year(s) Of Engagement Activity 2018
 
Description Determinants of antigenicity of H9 Avian Influenza Viruses. UK-China Swine and Poultry Workshop.17-18 June 2019, The Pirbright Institute, UK. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact The talk and the discussion on the improvement of disease control systems (vaccines and diagnostics) to prevent avian influenza viruses infection in poultry and zoonotic infections in humans.
Year(s) Of Engagement Activity 2019
 
Description Diamond (TT) 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Diamond Light Source Open Day - explaining to general public the importance of structural biology and microscopy for understanding viruses and designing improved vaccines.
Year(s) Of Engagement Activity 2019
 
Description EUFMD 2016 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Policymakers/politicians
Results and Impact During the EuFMDV conference taking place from 26 to 28 October 2016, Paolo Ribeca gave a talk on "VIBAsys and FMDV-Tools: Practical resources for FMDV sequence analysis". The conference, organised by the EU, gathers most of the important actors in FMD control. The talk explained how the new resources being developed by the Integrative Biology and Bioinformatics on behalf of the FMDV World Reference Lab can be useful to the community
Year(s) Of Engagement Activity 2016
 
Description Evolution of H9N2 avian influenza virus under immune pressure. Presented at 10th International Symposium on Avian Influenza, 15th - 18th April 2018, The Grand Hotel, Brighton, UK. 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact The primary aim of the workshop was to forge long-term research partnerships between early-career researchers, livestock industry and national disease control authorities in the UK and the Philippines. In addition, the activity hopes provide information about the emerging and next generation diagnostic and vaccine strategies and their utilization to reduce the impact of viral diseases on livestock and poultry.
Year(s) Of Engagement Activity 2019
URL https://www.slideshare.net/zerep_cire/2019-newton-agham-researcher-links-workshop-vaccines-and-diagn...
 
Description Expert group meeting for PPR eradication at head quarters of WHO, Vienna, Austria, 2018 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Policymakers/politicians
Results and Impact In the FAO/PPR eradication meeting satya has presented work on PPR epidemiology and diagnosis. The talk was designed for the stakeholders and policy make to make aware on important points that are required for eradication of PPR
Year(s) Of Engagement Activity 2018
 
Description H9N2 avian influenza viruses: impact of evolutionary changes on virus antigenicity, receptor binding and zoonotic potential. Presented at "Conference on Animal Infectious Diseases and Human Health" held at The Veterinary Biotechnology Branch of Chinese Association of Animal Science and Veterinary Medicine & the Veterinary Immunology Branch of Chinese Society for Immunology. August 7th-10th, 2018 Harbin, China. 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Focus of the conference was to improve control against infectious diseases affecting poultry and livestock.
Year(s) Of Engagement Activity 2018
 
Description H9N2 avian influenza viruses: impact of evolutionary changes on virus antigenicity, receptor binding and zoonotic potential. Presented at "Conference on Animal Infectious Diseases and Human Health" held at The Veterinary Biotechnology Branch of Chinese Association of Animal Science and Veterinary Medicine & the Veterinary Immunology Branch of Chinese Society for Immunology. August 7th-10th, 2018 Harbin, China. 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Dissemination of research outcomes to prevent and control of infectious diseases of animals and humans.
- Avian influenza virus evolution and efficacy.
-
Year(s) Of Engagement Activity 2018
 
Description Hands-on experiment for students at Gordon School, Woking, Surrey 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Three scientists from Pirbright (BA, DK, EH) ran a practical session at Gordon School in November 2018 to allow students to be able to trial new rapid diagnostics assays for transboundary diseases.
Year(s) Of Engagement Activity 2018
 
Description Identification of antigenic epitopes to broaden and enhance the efficacy of avian influenza vaccines. Presented at 6th Animal Vaccines and immune Adjuvant Technologies Salon & Advanced seminar, Shandong-Binzhou Animal Science and Veterinary Medicine Academy, China, 18-20 October 2019. 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact The talk and the discussion was the best practices to improve control systems (vaccines and diagnostics) for prevention of avian influenza viruses infection in poultry and zoonotic infections in humans.
Year(s) Of Engagement Activity 2019
 
Description Immuno-pathobiology of H9N2 avian influenza viruses: looking at how the viruses evolve and persist in poultry. Presented at "Techniques for Healthy Farming and Diseases Prevention & Control of Livestock and Poultry. Beijing, August 13th-15th, 2018. 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Dissemination of research outputs. Improvement of vaccines and diagnostics.
Year(s) Of Engagement Activity 2018
 
Description Improving vaccines and diagnostics for Avian influenza viruses affecting poultry. Presented at the International Poultry Expo "Poultry Science Conference", Lahore, Pakistan, 13-15 September 2019. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact The talk and the discussion was the best practices to improve control systems (vaccines and diagnostics) for prevention of avian influenza viruses infection in poultry and zoonotic infections in humans.
Year(s) Of Engagement Activity 2019
 
Description Influenza update meeting (Pengxiang Chang) 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact presented work entitled: Antigenic characterization of avian influenza H7N9 virus by in vitro immune escape mutant selection method.
Year(s) Of Engagement Activity 2018
 
Description Initiation of a mass vaccination campain ( 2018) in TamilNadu, India 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact Scientists from The Pirbright Institute's Vaccine Differentiation group have recently returned from Chennai in India, where they conducted a mass peste des petits ruminants (PPR) vaccination campaign and awareness programme. They joined scientists from four specialist organisations; Tamil Nadu Veterinary and Animal Sciences University (TANUVAS), Indian Veterinary Research Institute (IVRI), National Institute of Animal Biotechnology (NIAB) and National Institute of Veterinary Epidemiology and Disease Informatics (NIVEDI).
Peste des petits ruminants (PPR), also known as goat plague, is highly contagious and infects small ruminants such as sheep and goats, causing up to 90% mortality. The disease is prevalent across large parts of Africa, the Middle East, India and China and is estimated to cost between US$1.4 billion and US$2.1 billion globally each year. In 2017, the Food and Agricultural Organization (FAO) and the World Organisation for Animal Health (OIE) introduced a global eradication programme to reduce the devastating impact PPR has on the economy and food security of affected countries.
"Over 40 vets joined our vaccination campaign in the Tanir Kulum village of Tiruvallur District, TaminNadu (close to Chennai), where we administered vaccines to over 400 sheep and goats in a single day. We also ran an awareness camp where we provided farmers and vets alike with expert guidance on diagnosing clinical signs of PPR and what measures they could take to reduce its spread", said Professor Satya Parida from Pirbright, who led the collaborative effort with Dr Dhinakar Raj from TANUVAS.
Year(s) Of Engagement Activity 2018
URL https://www.pirbright.ac.uk/news/2018/09/pirbright-scientists-run-vaccination-campaign-eradicate-pes...
 
Description Innovate Guildford (Angita Shrestha): 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact GENERAL SCIENCE
Year(s) Of Engagement Activity 2019
 
Description Innovate Guildford - DB & KC 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Public science event where team members participated on a Pirbright Institute stand which contained activities to help the public understand how scientists can help to contain and control viral outbreaks. Over 500 members of the general public attended which stimulated increased interest in science and research and led to requests for more information.
Year(s) Of Engagement Activity 2019
 
Description Innovate Guilford-AH, AA, ER, EL 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact General science festival engaging the public in the research undertaken at Pirbright
Year(s) Of Engagement Activity 2019
 
Description International Avian Respiratory Disease Conference 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact Michael presented his research at the 2018 International Avian Respiratory Disease Conference in the US to an audience of avian researchers and representatives form the poultry veterinary vaccine industry. This sparked discussion and potential future collaborations
Year(s) Of Engagement Activity 2018
 
Description International Conference 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Global Alliance for Research on Avian Diseases conference health in Hanoi 17-19 January 2018. Purpose of the conference was to establish global partnerships and links between academic researchers and the poultry industry to improve joined up work in regards to preventing, controlling and
combating avian diseases. The conferences provided a forum for discussion and exchange of ideas around the single theme of exploiting available opportunities to deal with emerging and existing infectious avian diseases in poultry production systems and to safeguard food supplies and human health.
Conference speakers discuused basic, applied and commercial aspects of research on avian diseases including:
• Evolution and Epidemiology of Avian Pathogens
• Host-Pathogen Interactions - Virulence and Pathogenicity
• Pathogen Mechanisms of Immune Evasion
• Next Generation Disease Control Strategies
• Vaccines
• Immunity
• Diagnostics
• Zoonoses and Socio-economic impacts of avian diseases
• Global Research Opportunities and Threats
Year(s) Of Engagement Activity 2018
URL http://www.theeventsportal.com/GARAD-2018/3/Home
 
Description International Day of Women and Girls in Science 
Form Of Engagement Activity Engagement focused website, blog or social media channel
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact I gave a quote as a STEM ambassador about why I think it is important to make sure women and girls have the same opportunities in STEM. This was shared on social media to celebrate International Day of Women and Girls in Science, a UN-led initiative.
Year(s) Of Engagement Activity 2019
 
Description International Women's Day video for social media 
Form Of Engagement Activity Engagement focused website, blog or social media channel
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact Three team members were interviewed about their careers in science for International Women's Day. The videos were posted on Pirbright's website, Facebook and Twitter to celebrate what we love about working in science.
Year(s) Of Engagement Activity 2018
 
Description Interview with BBC journalist for article on viral emergence in new species 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact I was interviewed by Jonathon Ball who was writing an article for the BBC on the emergence of morbilliviruses in new hosts and the importance of vaccination. I helped him to contextualise the story and put him in touch with my collaborators.
Year(s) Of Engagement Activity 2020
URL https://www.bbc.co.uk/news/science-environment-50839868
 
Description Invitation to give keynote talk on 'Control of avian diseases: Challenges and opportunities' 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Gave the Keynote talk on 'Control of avian diseases: Challenges and opportunities' at this International Symposium organized with an aim to gather international virology experts from both human and veterinary fields to share research experience, update findings and contribute their expertise through presentation and interactive discussion. The topics will cover a wide range of virology research common in both human and animal aspects. In addition, technological platforms such as vaccine development, and novel assays will be emphasized and addressed.
Year(s) Of Engagement Activity 2018
URL http://www.biotec.or.th/virus-symposium2018/index.php
 
Description Invitation to talk at the Vaccine Symposium "Vaccines at different levels of complexity from subunit to whole cell formulations" jointly organised by the Centre of Genomic Regulation (CRG), Radboud UMC and MSD Animal Health, with the support from the MycoSynVac project 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Gave a talk and participated in the discussion on the challenges and research opportunities for innovation in poultry disease control
Year(s) Of Engagement Activity 2017
URL http://www.crg.eu/
 
Description Invited Talk: Indian Veterinary Institute, Bareilly, India 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact SP delivered a talk at faculty on FMD vaccine strain selection in South East Asia including India
Year(s) Of Engagement Activity 2018
 
Description Invited talk at Agricultural Science congress at New Delhi, India, February-2019- Use of reverse genetics to study the early pathogenesis and to develop marker vaccines for PPR 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact 1. In a DBT-BBSRC FADH grant, The Pirbright Institute in collaboration with four Indian partners (TANUVAS, IVRI, NIAB and NIVEDI) has developed a PPR vaccine, which will be the first to differentiate between infected and vaccinated animals (DIVA), for which a patent application has been filed. Scientists have manipulated in the genome of PPR full length cDNA and rescued the DIVA vaccine virus using reverse genetic technique. Further this vaccine has been tested in goats providing full safety and potency upon virulent virus challenge. In contrast to current vaccine, this new DIVA vaccine can differentiate between naturally infected and vaccinated animals, therefore will help in meaningful assessment of vaccine coverage and epidemiological surveillance based on serology, in turn increasing the efficiency of control programmes.
2. Little is known about the early events in the development of peste des petits ruminants (PPR) virus (PPRV) infection. The current dogma is that similar to RPV, PPRV replicates primarily in the epithelium of the respiratory tract before disseminating throughout the host. However, our investigations, using intranasal inoculation of PPRV containing GFP (Green Fluorescent Protein) to mimic natural infection, indicate this is not the case and that immune cells in the pharyngeal tonsil is the primary target. This is a ground breaking discovery which changes the PPR pathogenesis. This infectious GFP virus is made from a synthetic cDNA of field virus (PPRV/Mor-08) using reverse genetics technique under FADH grant.
The presentation sparked questions and discussions after the talk. Two PPR DIVA vaccines developed and the mechanism of PPR virus pathogenesis explained.
Year(s) Of Engagement Activity 2019
 
Description Invited talk at the National Institute for Animal Biotechnology, Hyderabad on 3rd Jan 2019 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Professional Practitioners
Results and Impact Participants were engaged in the recent advances in biotechnology, challenges and opportunities.
Year(s) Of Engagement Activity 2019
 
Description Invited to talk at the International Conference on Building Human and Animal Health Capacities organised by the Jordan University of Science and Technology 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Participated in the discussion and talked on 'The recent advances in vaccination-based control of poultry diseases'. The need for increased awareness of the pathogen diversity in the design of vaccines and the opportunities from advances in vaccine technologies were discussed
Year(s) Of Engagement Activity 2017
URL http://www.just.edu.jo/conferences/bhahc/Pages/default.aspx
 
Description Isabelle Dietrich - Bishop David Brown School Careers Fair 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact >100 students attended careers fair. Informed students about The Pirbright Institute's work and career opportunities.
Year(s) Of Engagement Activity 2019
 
Description Isabelle Dietrich - Collingwood College Careers Fair 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact >100 students attended careers fair. Informed students about The Pirbright Institute's work and career opportunities.
Year(s) Of Engagement Activity 2019
 
Description Keynote Presentation: 6ème Journée Scientifique sur les Maladies Animales Transfrontalières, Tunisia 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Provided invited keynote presentation entitled "Épidémiologie de la Fièvre Aphteuse en Afrique du Nord" at the 6ème Journée Scientifique sur les Maladies Animales Transfrontalières, Gammarth, Tunisia, November 2018.
Year(s) Of Engagement Activity 2018
 
Description Keynote talk at the World Veterinary Poultry Association meeting 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Invited to give the keynote talk on 'Avian tumour viruses: their contribution to our understanding of cancer' at the World Veterinary Poultry Association XXth Congress in Edinburgh
Year(s) Of Engagement Activity 2017
URL http://www.wvpac2017.com/
 
Description Lorin Adams (Gordon's School Careers Fair) 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Describe the research that lead to improve control systems against infectious viral diseases of animals and from animals to humans.
Year(s) Of Engagement Activity 2019
 
Description MBPAV -presentation 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Postgraduate students
Results and Impact Molecular Biology and Pathogenesis of avian viruses conference - Oxford 3-4th September 2018. Oral presentation given on drug resistant influenza viruses to approximately 50 scientist. Increased collaborative approaches following presentation.
Year(s) Of Engagement Activity 2018
URL https://microbiologysociety.org/event/society-events-and-meetings/focused-meeting-2018-molecular-bio...
 
Description MSc student visit 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Postgraduate students
Results and Impact I hosted several MSc students from University of Surrey, discussed avian research with them and demonstrated some laboratory techniques. The students gained an understanding of academic research and laboratory work.
Year(s) Of Engagement Activity 2018,2019
 
Description Mapping antigenic determinants of H9N2 avian influenza viruses and improving vaccines and diagnostics" presented at Foreign Talent Lecture: New strategies and new techniques for the prevention and control of major avian diseases. Shandong Binzhou Animal Science & Veterinary Medicine Academy, Binzhou, China. 6th August 2018. 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact The focus of the meeting was to improve the control systems against poultry diseases including development of improved vaccines and diagnostics
Year(s) Of Engagement Activity 2018
 
Description Microbiology Society Annual conference (UK) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact Poster or oral presentation at Microbiology Society annual conference, a major international event. The participants included researchers from many different areas of science. This provides a platform to promote research to a wider audience as well as discuss the key findings with experts in my own field.
Year(s) Of Engagement Activity 2010,2011,2012,2013,2014
 
Description Microbiology Society Avian Focus Meeting (UK) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact I gave a 15 minute oral presentation to an audience of around 80 people in 2016 and 2018. The audience consisted of researchers from many different scientific institutions from different areas of avian research. This was a great opportunity to present to experts in this field and gain novel insights into the project and the possible applications of my work.
Year(s) Of Engagement Activity 2016,2018
 
Description Microsoc 2018 "Structure-guided identification of a pathogen with pandemic potential" poster 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Presented a poster on our research at the MicroSoc annual meeting
Year(s) Of Engagement Activity 2018
 
Description Molecular Determinants for Antigenicity and Vaccine Efficacy of H9 and H7 Avian Influenza Viruses. Newton Agham Researcher Links Workshop: Novel vaccines and diagnostics technologies against Emerging and re-emerging veterinary Pathogens. 4-7 February 2019 at Rizal Pak Hotel, Manila, Philippines. 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact The talk and the discussion on the improvement of disease control systems (vaccines and diagnostics) to prevent avian influenza viruses infection in poultry and zoonotic infections in humans.
Year(s) Of Engagement Activity 2019
 
Description Molecular determinants modulating avian influenza H7N9 virus antigenicity. Presented at The Microbiology Society Annual Conference 2019, 8-11 April ICC Belfast, UK. 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact The talk and the discussion on the improvement of disease control systems (vaccines and diagnostics) to prevent avian influenza viruses infection in poultry and zoonotic infections in humans.
Year(s) Of Engagement Activity 2019
 
Description Molecular determinants of antigenicity of H7 and H9 avian Influenza viruses. Presented as invited speaker at BBSRC UK-India Partnering Award seminar: University of Liverpool. 2 May 2019. 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact The talk and the discussion on the improvement of disease control systems (vaccines and diagnostics) to prevent avian influenza viruses infection in poultry and zoonotic infections in humans.
Year(s) Of Engagement Activity 2019
 
Description Munir Iqbal: Cheltenham Science Festival 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Describe the research that lead to improve control systems against infectious viral diseases of animals and from animals to humans.
Year(s) Of Engagement Activity 2019
 
Description Negative Strand Virus 2018 Meeting "Structure-guided identification of morbilliviruses with zoonotic potential" Poster 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Presented a poster on the Structure-guided identification of morbilliviruses with zoonotic potential at the international negative strand virus (NSV) meeting in Verona, Italy.
Year(s) Of Engagement Activity 2018
 
Description Newton Agham Researcher Links Workshop on "Novel Vaccines and Diagnostic Technologies Against Emerging and Re-emerging Veterinary Pathogens, Rizal Park Hotel, Manila, Philippines 4-7 February 2019 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact The primary aim of this workshop was to forge long-term research partnerships between early-career researchers, livestock industry and national disease control authorities in the UK and the Philippines. The workshop provided information on emerging and next generation diagnostic and vaccine strategies and their utilization to reduce the impact of viral diseases on livestock and poultry.
Year(s) Of Engagement Activity 2019
URL https://www.slideshare.net/zerep_cire/2019-newton-agham-researcher-links-workshop-vaccines-and-diagn...
 
Description Nidovirus symposium 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact I presented my work at the Nidovirus Symposium, which is held every three years and brings together researchers from industry and academia from many different countries. I had some interesting discussions with other researchers and built my network.
Year(s) Of Engagement Activity 2017
 
Description Offer Paper oral presentation "Rapid Mammalian adaptation of H9N2 avian influenza viruses" at Microbiology society annual conference 4th April 2017 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Other audiences
Results and Impact Offer Paper oral presentation "Rapid Mammalian adaptation of H9N2 avian influenza viruses" at Microbiology society annual conference 4th April 2017. Talk in the main virus symposia with an audience of approximately 200 researchers (PhD students through to Professors) mainly based in UK but some international. Discussions with other researchers around this area of work and increase in national profile of groups work.
Year(s) Of Engagement Activity 2017
 
Description Oral Presentation at the Microbiology Society conference - Title: Application of a deep-sequencing protocol to identify immune escape variants of FMDV 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Postgraduate students
Results and Impact Oral Presentation of my reseach activities to other PhD students and postdoctoral scientist.
Year(s) Of Engagement Activity 2018
 
Description Oral and poster presentation at European Society of veterinary Virology and EPIZONE (ESVV/EPIZONE) at Vienna, Austria 2018 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact Presentation has been made on early events of PPR virus pathogenesis. The presentation reveled that PPR virus first taken up by the immune cells to the Pharyngeal tonsil and then to other lymph nodes before generalisation of the disease. Secondary replication happens in epithelial cells. This alters the existing believe that virus primarily replicate in the epithelial cells.
Similarly a poster presentation has been done to show the detection of nucleic acid of PPR virus during PPR eradication programme, nasal sample has been shown as the best sample for viral genome detection.
Year(s) Of Engagement Activity 2018
 
Description Oral preserntation at Influenza update Meeting in Edinburgh 21st November 2017 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Other audiences
Results and Impact Presentation of "Rapid Mammalian adaptation of H9N2 virus" to Influenza researchers attending the 2 day Influenza viruses meeting at the Roslin institute in Edinburgh.
Year(s) Of Engagement Activity 2017
 
Description Organisation of Influenza meeting at Roslin Institute 21st-22nd November 2017 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Other audiences
Results and Impact Along with Paul Digard (Edinburgh) and Edward Hutchinson (Glasgow) I helped to organize, plan and run a Influenza update meeting which ran for 2 days at The Roslin Institute in November 2017. The symposium was attended by approximately 60 influenza researchers from the UK. Talks were given by a range of people with student talks and posters being awarded prizes. This was supported by Medimmune and the Microbiology Society.
Year(s) Of Engagement Activity 2017
 
Description Organisation of UK-India Bioinformatics Workshop 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact The meeting discussed the challenges and opportunities and have decided to come up with a strategy document on working together between UK and India on data-driven biology
Year(s) Of Engagement Activity 2018
 
Description Organised the 3rd UK-China CERAD meeting in Guildford, UK in July 2017 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact The 3rd UK-China CERAD meeting helped to bring together professional from both countries to identify challenges and exploit the opportunities for improved control of poultry diseases and increasing food security.
Year(s) Of Engagement Activity 2017
URL http://www.uk-china-cerad.org/
 
Description Organising and giving Keynote talk at the GARAD meeting 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Global Alliance of Poultry industry professional and academia to discuss about the advances in control strategies of diseases
Year(s) Of Engagement Activity 2018
URL http://Garad.org
 
Description PDRA in my group Stacey Human attending Cheltenham Science Festival 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Stimulating increased interest in science and research
Year(s) Of Engagement Activity 2019
 
Description PPR eradication meeting organised by FAO and OIE at Rome and talk has been delived on Development of DIVA vaccines 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact Satya Parida is working as an expert to PPR eradication and he is being invited for few of FAO/OIE meetings. The meetings discussed about roadmap, vaccine production capacity and eradication policy.
Satya also presented work on PPR DIVA vaccine and DIVA tests developments and their evaluation in goats.
Year(s) Of Engagement Activity 2018
 
Description PPR vaccine producers meeting at MCI, Morocco organised by FAO/OIE 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact PPR vaccine producers meeting at MCI, Morocco organised by FAO/OIE. Almost all PPR vaccine producers through out world joined this meeting. Satya Parida has presented a talk on vaccine development and transmission of PPR virus in North Africa. There was hues interest on the talk particularly the spread of PPR in North Africa and new vaccine development.
Year(s) Of Engagement Activity 2017
 
Description PPR-Global Research and Expertise Network talk "Examining the zoonotic potential of PPRV" Vienna 2018 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Policymakers/politicians
Results and Impact Gave a talk to the inaugural meeting of the PPR Global Research and Expertise Network (PPR GREN) at IAEA headquarters in Vienna, Austria. Participants included policy makers from the FAO and OIE.
Year(s) Of Engagement Activity 2018
 
Description Paolo's talk on FMDV vaccine matching 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Policymakers/politicians
Results and Impact Paolo Ribeca gave a talk at the 2018 Open Session of EuFMD (European commission for the control of foot-and-mouth disease ) on "Effective in-silico sequence-based prediction of FMDV vaccine matching" on behalf of Yasaman Kalantar-Motamedi, Mana Mahapatra, Luca Ferretti, Sophie Mahendran, Satya Parida, and himself.
Year(s) Of Engagement Activity 2018
 
Description Pengxiang Chang: Talk presentation:The application of CRISPR/Cas9 system in the generation of viral vectored avian influenza vaccines at Microbiology Society conference, UK. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact The talk and the discussion on the improvement of vaccines to prevent avian influenza viruses infection in poultry and zoonotic infections in humans.
Year(s) Of Engagement Activity 2019
URL https://microbiologysociety.org/event/annual-conference/annual-conference.html
 
Description Pengxiang Chang: UK-China Newton Fund (Poultry and swine) workshop at The Pirbright Institute.Oral presentation:Avian Influenza H7N9 Antigenic markers 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact The talk and the discussion on the improvement of disease control systems (vaccines) to prevent avian influenza viruses infection in poultry and zoonotic infections in humans.
Year(s) Of Engagement Activity 2019
 
Description Pengxiang Chang:Influenza update meeting. The application of CRISPR/Cas9 in the development of vaccines against avian influenza virus 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Described techniques to improve and produce new effective vaccines against viral diseases of animals and humans.
Year(s) Of Engagement Activity 2019
 
Description Pirbright Dragon Fair - MZ 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Team members attended the locally held Dragon fair where the Pirbright Institute had a stand explaining the types of research that are conducted at the Institute, making our high containment science activities transparent to the local community. Approximately 60 people engaged with the stall to learn about the science happening on their doorstep. This stimulated interest in science and research.
Year(s) Of Engagement Activity 2019
 
Description Post presentation at the EUROPiC conference - Title: Optimisation of a deep-sequencing protocol to identify low frequency variants of foot-and-mouth disease virus 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Poster presentation in regards to my PhD research in a workshop to other PhD students and Postdoctoral scientist.
Year(s) Of Engagement Activity 2018
 
Description Postdoctoral research Carina Conceicao poster at Microsoc 2019 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Carina presented data on her work about morbillivirus host range
Year(s) Of Engagement Activity 2019
 
Description Postdoctoral researcher Stacey Human talk at Microsoc 2019 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Stacey presented her data on examining the cellular processes involved in trafficking RSV components to the cell surface.
Year(s) Of Engagement Activity 2019
 
Description Poster presentation at EUFMD meeting at Burgo by the senior postc of the PI group on epitope prediction of serotype O FMD virus 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact In this presentation we have shown 3 new epitopes of the FMD virus. An manuscript has been submitted for publication on this.
Year(s) Of Engagement Activity 2018
 
Description Poultry Health course 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Poultry Health Course is run as a residential and online course to transfer recent advances in avian disease research from the laboratory to the field.
Year(s) Of Engagement Activity Pre-2006
URL http://www.poultryhealthcourse.com/
 
Description Poultry Stakeholders meeting hosted by Al Dousari Veterinary Services and Agriculture Co (Qatar ) in Qatar, 15 October 2019 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact The focus of talk was best practices for prevention and control of Avian influenza disease outbreaks in poultry

Vaccines and diagnostics for the control of avian influenza in poultry
Year(s) Of Engagement Activity 2019
 
Description Poultry Stakeholders meeting hosted by Almarai, 15 October 2019 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Engagement with Almarai Poultry. The focus of the meeting with Almarai Poultry disease control team was to share knowledge on improved vaccination and diagnostic techniques against avian influenza viruses affecting poultry production.
Year(s) Of Engagement Activity 2019
 
Description Poultry Stakeholders meeting in Kuwait city 16 October 2019 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Prevention and control of avian influenza outbreaks in poultry.
Described new vaccination and diagnostic technologies that provide more robust cross-protective immunity in emerging antigenic divergent strains of avian influenza viruses.
Year(s) Of Engagement Activity 2019
 
Description Presentation at Global Alliance for Research on Avian Diseases Conference 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact Presentation of my research at the Global Alliance for Research on Avian Diseases Conference in Vietnam to approximately 80 conference participants resulted in discussions about future research directions.
Year(s) Of Engagement Activity 2018
 
Description Presentation at RIVR meetings 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Other audiences
Results and Impact Presentation of my research at Recently Independent Virology Researchers (RIVR) meetings in 2016, 2017, 2018 and 2019. I discussed my work and future collaborations with other virology researchers working in the UK.
Year(s) Of Engagement Activity 2016,2017,2018,2019
 
Description Presentation on the effects on oncogenic virus infection at the Veterinarian's Forum, Namakkal, India on 29 Dec 2018 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Professional Practitioners
Results and Impact The purpose of the engagement was to update the practicing poultry veterinarians on the problems with oncogenic virus diseases and methods of control. The meeting attended by nearly 100 veterinarians was very informative and ended with a long discussion on the challenges and innovations
Year(s) Of Engagement Activity 2018
 
Description Presentation to BBSRC Executive Board 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Other audiences
Results and Impact I presented our research to the BBSRC Executive Board when they visited The Pirbright Institute in September 2018 and discussed the impacts of our work..
Year(s) Of Engagement Activity 2018
 
Description Project close meeting at TANUVAS, Chennai July 2018- Delivering talk on outcome of the project 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact The project team held their final workshop at TANUVAS, which concluded a four year Farmed Animal Disease and Health (FADH) grant joint funded by the Biotechnology and Biological Sciences Research Council (BBSRC) and the Indian Government's Department of Biotechnology (DBT). "This grant has enabled us to collaborate with four organisations across India, and ensured that our research can be applied in the field to aid the campaign for PPR global eradication", said Professor Parida.The project covered many areas of research which are essential for understanding PPR and creating tools to help control and prevent the disease. The team have now filed a patent application for their newly developed PPR vaccine, which is the first to differentiate between vaccinated and infected animals (DIVA) - a quality that enables livestock owners to protect their animals whilst continuing to trade.
The team have also investigated how the PPR virus (PPRV) infects sheep and goats and how their immune systems respond. By inserting green fluorescent protein into virulent PPRV and administering the modified virus to goats, they demonstrated that PPRV primarily infects the tonsils, challenging the earlier belief that the virus first replicates in the respiratory tract epithelial cells. The collaborative project has also generated better diagnostic tests for use in the field and laboratory, and preliminary research has identified why some Indian breeds of goats and sheep are resistant to the disease, which could help scientists to create PPRV resistant breeds in the future.
Project partners, scientists from the University and 40 field veterinarians have joined the meeting. An awareness training has been conducted on PPR disease and eradication for these field veterinarians.
Year(s) Of Engagement Activity 2018
URL https://www.pirbright.ac.uk/news/2018/09/pirbright-scientists-run-vaccination-campaign-eradicate-pes...
 
Description Protecting poultry from avian influenza through passive immunisation. Presented at Foreign Experts Seminar "New strategies and new tools for the prevention and control of major poultry diseases. Shandong-Binzhou Animal Science and Veterinary Medicine Academy, China, 20-22 September 2019. 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact The talk and the discussion was the best practices to improve control systems (vaccines and diagnostics) for prevention of avian influenza viruses infection in poultry and zoonotic infections in humans.
Year(s) Of Engagement Activity 2019
 
Description Public debate on Zoonotic threat at Cheltenham Science Festival 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact I was part of a panel debate on the threat of zoonosis and viral emergence in human populations, a ticketed event attended by around 150 members of the general public.
Year(s) Of Engagement Activity 2019
 
Description Rebecca Daines: Cheltenham Science Festival 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Described the research undertaken at the Pirbright Institute that lead to improve control systems against infectious viral diseases of animals and from animals to humans.
Year(s) Of Engagement Activity 2019
 
Description Recombinant single chain variable fragment (scFv) antibody protects chickens from avian influenza. Presented at Systems Immunology Workshop: Adaptive immune repertoires and beyond. 16th-17th January 2020. University of Surrey, UK 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact A talk entitled "Recombinant single chain variable fragment (scFv) antibody protects chickens from avian influenza" was presented at the workshop exploring new technical advances for analysis and production of antibodies for prophylaxes and treatment of animal and humans diseases. Both academics and staff linked with animal and public health industry attended this workshop.
Year(s) Of Engagement Activity 2020
 
Description Research Assistant in my group Nazia Thakur attending Cheltenham Science Festival 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Stimulating increased interest in science and research
Year(s) Of Engagement Activity 2019
 
Description Reverse genetics seminar (University of Surrey) 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Postgraduate students
Results and Impact A seminar was given based upon the reverse genetics system of infectious bronchitis virus. The audience was a group of 10 -15 MSc students from University of Surrey. The students were engaged, asked questions about the research and careers in science. We were asked to repeat the seminar for the next year's intake of students.
Year(s) Of Engagement Activity 2013,2014,2015,2016,2017
 
Description SATRO Mentoring Scheme Ash Manor School 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Fortnightly mentoring activity for student's soft skill and career development.
Year(s) Of Engagement Activity 2019,2020
 
Description STEAMfest - HS 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Schools
Results and Impact Team members attended the event where a flu fighters stand was displayed demonstrating how scientists are working to rapidly detect and control avian influenza in the filed to protect food security, animal welfare and human health. Approximately 200 secondary school students engaged with the stall and this stimulated increased interest in science and research,
Year(s) Of Engagement Activity 2019
 
Description School Workshop 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Participated (AES) in Workshop at local school
Year(s) Of Engagement Activity 2019
 
Description Sue Baigent participated in the Teen Tech event on 22nd Jan 2019 on General Science engagement 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact A General Science event
Year(s) Of Engagement Activity 2019
 
Description Sue Baigent participated in the Winston Churchill Careers Fair on 7th Nov 2018 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Careers fair
Year(s) Of Engagement Activity 2018
 
Description Surrey County Show 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact The Surrey County Show is an agricultural show in Guildford that is visited by the general public. The Pirbright Institute had a stand with various activities for visitors to take part in and explain our science to a wide audience. Giulia spent a day working on the stand and spoke with many children and adults about her work at Pirbright to change public attitudes and stimulate increased interest in research.
Year(s) Of Engagement Activity 2018
 
Description Surrey County Show 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact This engagement activity was associated with a stand at the Surrey County Show in May 2018 where the work of the Institute was show-cased to the general public.
Year(s) Of Engagement Activity 2018
 
Description Sushant Bhat:Pirbright Dragon Fair 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact Described the research undertaken at the Pirbright Institute that lead to improve control systems against infectious viral diseases of animals and from animals to humans.
Year(s) Of Engagement Activity 2019
 
Description Symposium for WRLFMD 60th Anniversary 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact During November, the Pirbright Institute hosted a two-day symposium attended by over 100 FMD scientists to celebrate the 60-year anniversary of the establishment of the World Reference Laboratory for Foot-and-Mouth Disease (WRLFMD).

The meeting was opened by Christine Middlemiss (UK, Chief Veterinary Officer), Matthew Stone (Deputy Director General of the OIE) and Keith Sumption (from FAO/EuFMD). Subsequent scientific sessions reviewed the current activities of the WRLFMD (and OIE/FAO FMD Laboratory Network: https://www.foot-and-mouth.org), and discussed new opportunities to monitor, diagnose and control FMD by applying knowledge from fundamental research projects that aim to understand the pathogenesis, host responses and epidemiology of the disease.

Talks from invited speakers and WRLFMD staff were within four themes of (i) Risks & Threats, (ii) Diagnostic Innovation, (iii) Pathogenesis & Immunity and (iv) FMD Control with a Focus on Vaccination. These presentations showcased the synergy between fundamental/basic science and the on-going global surveillance activities undertaken by FMD Reference Laboratories.

In the evening, the meeting delegates (including past and present members of the WRLFMD) were royally entertained at a local hotel by after-dinner "trips down memory lane" talks from Nick Knowles and Dave Rowlands that highlighted key events and personalities that have shaped the history of work at Pirbright.
Year(s) Of Engagement Activity 2018
URL https://www.wrlfmd.org/60th-anniversary
 
Description TAIEX Multi Country Workshop on Animal Health 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Policymakers/politicians
Results and Impact Donald King was an invited speaker (and expert panel member) at a TAIEX Multi Country Workshop on Animal Health, Malta, June 2017. He gave a talk entitled: foot-and mouth disease: epidemiological situation in the Mediterranean area and new threats to North Africa, Near East and Europe
Year(s) Of Engagement Activity Pre-2006,2017
URL https://www.eu2017.mt/en/Events/Pages/TAIEX-multi-country-seminar-on-animal-health.aspx
 
Description TK - School activity - Big Bang at Surrey University (7 Feb 18) 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Children from local primary and secondary school attend and access stalls showcasing science and engineering. The Pirbright Institute stall showcases the requirement for improved control of avian influenza viruses with a hands on display of testing and diagnosis of the virus. This sparks questions and discussion about the work at Pirbright in the area of avian influenza viruses.
Year(s) Of Engagement Activity 2018
URL https://nearme.thebigbangfair.co.uk/
 
Description TV Interview for SBS, Korea 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact Don King provided a TV interview to SBS, Korea to review the FMD situation in South Korea and discuss policies used for disease control
Year(s) Of Engagement Activity 2017
 
Description Talk by Nick on the sequencing of Zimbabwe FMDV 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Policymakers/politicians
Results and Impact Nick Knowles gave a talk at the 2018 Open Session of EuFMD (European commission for the control of foot-and-mouth disease ) on "Complete Genome Sequence Analysis of Over 140 Foot-and-mouth Disease Viruses Isolated From Free-living African Buffalo (Syncerus Caffer) in Zimbabwe" on behalf of Jemma Wadsworth, Bruce Bolt, Luca Ferretti, Euan C. Anderson, Ashley Gray, Paolo Ribeca, and himself. The sequencing was funded by the Transboundary Pathogens portal project awarded to Pirbright.
Year(s) Of Engagement Activity 2018
 
Description Teen Tech 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Schools
Results and Impact Participated in Teen Tech to engage with schools (year 8 and sixth formers) to encourage them to consider STEM career choices.
Year(s) Of Engagement Activity 2019
 
Description Teentech 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact The annual Teentech student science event in Guildford aims to encourage teenagers to consider science subjects and science careers. Our stand had considerable interest from the schoolchildren who were very enthusiastic. The event stimulated an increased interest in science and research.
Year(s) Of Engagement Activity 2016,2019
 
Description The Pirbright Village Duck Fair held on 16 June 2018 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact Local Village Fair, interaction with local residence to expanse research aims and activities of The Pirbright Institute and benefits to UK and around the globe to prevent and control infectious diseases of animals and humans.
Year(s) Of Engagement Activity 2018
 
Description The co-Circulation of Low and High-path-AIV in the same country with sub-optimal bio-security: Virus evolution and impact on vaccine efficacy. Presented at the 11th Scientific conference of Egyptian Veterinary Poultry Association, Conference "Towards a National strategy to control poultry industry problems in Egypt". 20-23 April 2019. 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact The talk and the discussion on the improvement of disease control systems (vaccines and diagnostics) to prevent avian influenza viruses infection in poultry and zoonotic infections in humans.
Year(s) Of Engagement Activity 2019
 
Description The global Situation and pathobiology of Avian Influenza in different Avian Species. Presented at the 11th Scientific conference of Egyptian Veterinary Poultry Association, Conference "Towards a National strategy to control poultry industry problems in Egypt". 20-23 April 2019.. 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact The talk and the discussion on the improvement of disease control systems (vaccines and diagnostics) to prevent avian influenza viruses infection in poultry and zoonotic infections in humans.
Year(s) Of Engagement Activity 2019
 
Description This Week In Virology Podcast 532: Morbillivirus had a little lamb 
Form Of Engagement Activity A broadcast e.g. TV/radio/film/podcast (other than news/press)
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Our research paper on morbillivirus host-range in Journal of Virology was highlighted for in-depth discussion on this episode of TWIV where they spent 30+ minutes talking about the work and its potential impact. This podcast is the number one podcast in virology and listened to across the globe.
Year(s) Of Engagement Activity 2019
URL http://www.microbe.tv/twiv/twiv-532/
 
Description This week in Virology: 533: Recurring threads 
Form Of Engagement Activity A broadcast e.g. TV/radio/film/podcast (other than news/press)
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Following our paper being highlighted on the previous edition of TWIV I wrote a letter to further the discussion of this subject and clarify some technical points on The Pirbright Institute. This was read out as part of the podcast and discussed.
Year(s) Of Engagement Activity 2019
URL http://www.microbe.tv/twiv/twiv-533/
 
Description Tour of Pirbright Research facilities for CLA group 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact The CLA is the membership organisation for owners of land, property and businesses in rural England and Wales. The CLA came for a tour of the Jenner building facilities at The Pirbright Institute. I led a tour of our building for the group and described the work undertaken by the avian research programme here.
Year(s) Of Engagement Activity 2017
 
Description U3A talk 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact Presentation on influenza virus research at The Pirbright Institute for the local University of the Third Age group, approx. 50 people aged 65 years + all with an interest in science.
Year(s) Of Engagement Activity 2018
URL https://cu3asandt.wordpress.com/2018/09/23/meeting-2nd-october/
 
Description UK-China Philippines-Thailand Swine and Poultry Research Initiative project kick-off workshop" at China Agriculture University, Beijing, 26th-28th September 2018. 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Emerging new approaches were discussed for prevention and control of infectious diseases affecting swines and poultry.
Year(s) Of Engagement Activity 2018
 
Description UK-India bioinformatics workshop 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact On February 9 2018 Pirbright organised a "UK-India bioinformatics workshop" in order to foster ties between Indian and UK bioinformaticians working in the field of livestock and crop research. Paolo Ribeca gave a talk on "Bioinformatics and sequencing at Pirbright".
Year(s) Of Engagement Activity 2018
 
Description Understanding Antigenic Diversity, Zoonotic potential and immunological prevention of avian influenza viruses affecting poultry. Presented at "UK-China Philippines-Thailand Swine and Poultry Research Initiative project kick-off workshop" at China Agriculture University, Beijing, 26th-28th September 2018. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Meeting focus was to share research plans funded under "UK-China-Philippines-Thailand Swine and Poultry Research Initiative". Research projects investigate pathogens, zoonotic pathogens or "commensals" associated with food safety issues affecting poultry and swine.
Year(s) Of Engagement Activity 2018
URL https://bbsrc.ukri.org/funding/filter/joint-swine-poultry-initiative/
 
Description Understanding antigenic diversity, zoonotic potential and immunological prevention of avian influenza viruses affecting poultry. Presented at UK-China-Philippines-Thailand Swine and Poultry Research Initiative: Interim Project Workshop 13th-15th January 2020 Hilton Hotel, Bracknell, UK 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Outcomes of the research project entitled "Understanding antigenic diversity, zoonotic potential and immunological prevention of avian influenza viruses affecting poultry" were presented to colleagues working on prevention and control of infectious diseases affecting animals and humans. the work led to incite further research collaborations with different groups from UK, China, Thailand and Philippine.
Year(s) Of Engagement Activity 2020
 
Description Understanding avian influenza virus evolution and development of next generation vaccines. Presented at 459th Scientific Meeting of The Veterinary Research Club at The Farmers Club, 3 Whitehall Court London Friday 14th February 2020. 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact A talk presented entitled"Understanding avian influenza virus evolution and development of next generation vaccines". The discussion with senior staff from DEFRA and UKRI- BBSRC, researchers and veterinary professional like with control and prevention of animal diseases in United kingdom
Year(s) Of Engagement Activity 2020
 
Description Understanding avian influenza virus evolution, antigenic diversity and vaccine failure. Presented at the 2019 Symposium on techniques for healthy breading and disease prevention & control of livestock and poultry, Golden Harbor Hotel, Beijing, 21-23 August 2019. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Talk and discussion on the control of infectious animal diseases including avian influenza.
Year(s) Of Engagement Activity 2019
 
Description V Nair invited to talk on 'Cell type- and disease stage-specific interactions of Marek's disease virus & the host' at the International Symposium on Marek's disease and avain herpesiviruses at Yangzhou, China during 29th July to 2nd Aug 2018 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Most recent updates in the field of avian herpesvirus research was discussed and active engagements for future collaborations discussed
Year(s) Of Engagement Activity 2018
 
Description V Nair was invited to talk a the Poultry Health Workshop and Conference on 19th-20th Nov, 2018 in Kathmandu, Nepal 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact more than 100 stakeholders from different sectors of the Poultry industry and academia attended the meeting. There was engagement from the different sectors for initiating a combined approach for finding solutions
Year(s) Of Engagement Activity 2018
URL http://www.cmdn.org.
 
Description Veterinary Times article on morbillivirus vaccines. 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact I was interviewed by a journalist from The Veterinary Times about our recent Journal of Virology research paper on morbillivirus host-range. This was then written up for the next edition of this publication.
Year(s) Of Engagement Activity 2019
URL https://www.vettimes.co.uk/news/scientists-multi-purpose-ppr-vaccine-closer-to-completion/
 
Description Visit to Cairo Poultry Company (CPC) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Industry/Business
Results and Impact Discussion and talk on prevention and control of poultry diseases (avian influenza and Newcastle Disease) and molecular factors affecting vaccine efficacy. The discussion and talk to present data, describing factors the directly influence protective efficacy of in use vaccines, and how they evaluate/ test vaccine failure.
Year(s) Of Engagement Activity 2019
 
Description Visit to Dakahlia Poultry Egypt 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Industry/Business
Results and Impact Discussion and talk presented at Dakahlia Poultry Egypt head office to workers involved in poultry production. The talk amis were to provide update on the impact of evolutionary genetic changes in the avian influenza and Newcastle disease viruses on the efficacy of vaccines and diagnostics. The molecular changes in virus genetic makeup allow virus to escape from vaccine induced immunity results vaccine and diagnostics failure. Consequently the avian influenza viruses continue to circulates in vaccinated flocks.
Year(s) Of Engagement Activity 2019
 
Description WACCBIP Ghana (TT) 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact West African Centre for Cell Biology of Infectious Pathogens (WACCBIP), University of Ghana. Workshops on cell biology of pathogens and career development.
Year(s) Of Engagement Activity 2018,2019
 
Description World Zoonosis Day 2018 - web story 
Form Of Engagement Activity A magazine, newsletter or online publication
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Contributed to an article from our institute on World Zoonosis Day highlighting the importance of research in this area.
Year(s) Of Engagement Activity 2018
URL https://www.pirbright.ac.uk/news/2018/07/pirbright-scientists-are-developing-vaccine-candidates-anim...
 
Description organised workshops at Microbiology Society annual conferences 2014 - 2018 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact I co-organised virology division workshops on viral evolution and diversity and RNA viruses with colleagues from the virology division. The workshops were held at the annual Microbiology Society conferences in spring each year. Organisation involved selecting abstracts for oral or poster presentation, ordering the presentations and chairing the sessions.
Year(s) Of Engagement Activity 2015,2016,2017,2018