Low Containment

Description	The key development within low containment science at The Pirbright Institute is that of providing facilities that allow handling of non-infectious and low pathogenicity agents within laboratories that are safe for the user and environment and with minimal interruptions to work. Within the first two years of reporting, these objectives have been achieved to the standards within the Impact Plan for this project in the following ways: 1) Interruptions to science are within the boundaries set for the facility. 2) Pirbright remains a hub for Avian research as demonstrated by the production of publications and outreach activities. While the SPF facility construction project has been delayed, contingency plans for research are in operation and the building is due to open in 2019. 3) Relationships with the National Avian Research Facility remain open and were reinforced by meetings that included BBSRC (UKRI) members. 4) Bioinformatics capabilities have been strengthened significantly with expansion of computing facilities and staff, the latter provisioned from the Science Services CCG component. 5) Significant progress has been made in opening up the low containment facilities for training and also in provision of outreach activities. 6) Insectary facilities, which have seen a significant rise in demand within the science conducted by Pirbright have been expanded via refurbishment of the IS4L facility. This is a medium term, interim solution and dedicated facilities will be required in the longer term. In total, 65 peer-reviewed publications and 220 engagement activities have been enabled by this infrastructure, including a major focus on Avian research and viruses spread by arthropod vectors.
Exploitation Route	Building of the Avian SPF facility remains a major component of the Pirbright suite of buildings and will be a major focus of research in this field both for internal and external workers. In addition, the interim insectary facilities will enhance workers abilities to carry out research on exotic and in some cases transgenic arthropods which is key future demand for the site. Keeping pace with the health and safety and biosecurity concerns around these studies will also influence the wider community as evidenced by the close relationship with HSE on guidelines for arthropod containment.
Sectors	Agriculture, Food and Drink

Description	Minimal infrastructure downtime enabled by planned preventative maintenance on the BBSRC National Vaccinology Centre (Jenner Building) has enabled our research to be conducted under expected timescales and provided additional space for external workers. In addition, the provision of interim insectary facilities has provided a medium-term solution to a lack of space, without major disruption to work programmes, although a more permanent solution will be required in the long-term.
First Year Of Impact	2017
Sector	Agriculture, Food and Drink
Impact Types	Societal,Economic,Policy & public services

Description	BBSRC 18ALERT panel member
Geographic Reach	National
Policy Influence Type	Participation in a advisory committee
Impact	Provision of multi-user equipment to core facilities to enable BBSRC remit science. Part of the remit for this call was to allow shared access from other institutions, widening the impact of BBSRC investment.

Description	BBSRC-Newton Fund Agricultural Data Re-use Advisory Group
Geographic Reach	National
Policy Influence Type	Participation in a advisory committee
Impact	Advice on re-usability of data generated in research projects.

Description	Coalition for Epidemic Preparedness Innovations Nipah Taskforce Member
Geographic Reach	Multiple continents/international
Policy Influence Type	Participation in a advisory committee

Description	Contributed to Chief Medical Officers report- Genomics section
Geographic Reach	Europe
Policy Influence Type	Implementation circular/rapid advice/letter to e.g. Ministry of Health
URL	https://assets.publishing.service.gov.uk/government/uploads/system/uploads/attachment_data/file/6310...

Description	Contribution to a POSTnote on 'Reducing UK Antibiotic Use in Animals'
Geographic Reach	National
Policy Influence Type	Participation in a advisory committee

Description	Human Infection Challenge Models for Vaccination
Geographic Reach	Europe
Policy Influence Type	Influenced training of practitioners or researchers

Description	IUCN Technical Subgroup on Synthetic Biology and Biodiversity Conservation
Geographic Reach	Multiple continents/international
Policy Influence Type	Participation in a advisory committee

Description	Inter-institute imaging network
Geographic Reach	National
Policy Influence Type	Participation in a advisory committee

Description	Member of RMS EM Section committee
Geographic Reach	Multiple continents/international
Policy Influence Type	Membership of a guideline committee
Impact	This committee informs the RMS on which areas to invest funds in regarding training, conferences, outreach events. RMS members are international.

Description	Member of the British Society for Immunology Forum
Geographic Reach	National
Policy Influence Type	Influenced training of practitioners or researchers
URL	https://www.immunology.org/

Description	Netherlands Commission on Genetic Modification (COGEM) Project "Gene drives, inventory of previous research and experience"
Geographic Reach	Europe
Policy Influence Type	Gave evidence to a government review

Description	POSTnote Report on Climate and vector-diseases in humans in the UK
Geographic Reach	National
Policy Influence Type	Gave evidence to a government review

Description	PPR Global Research and Expertise Network (PPR GREN) membership (OIE/FAO)
Geographic Reach	Multiple continents/international
Policy Influence Type	Participation in a advisory committee

Description	Publication policy document for core facilities - EM-UK
Geographic Reach	Multiple continents/international
Policy Influence Type	Influenced training of practitioners or researchers
Impact	I am a founder member of the network created for electron microscopists within UK, EM-UK. The annual meeting is highly productive, and training and managing core facilities is regularly discussed. Recognition of these facilities is essential for their continued existence so we produced a generic document hosted on the RMS website which can be adopted by any core facility in any institution, and describes the publication policy which users had to adhere to. This has already raised the profile of core facilities around the country and is a successful outcome from the EM-UK meetings.

Description	RCUK Engagement workshop, Cosener's House, 20-21 April 2017
Geographic Reach	National
Policy Influence Type	Influenced training of practitioners or researchers

Description	Royal Microscopical Society Training Focussed Interest Group
Geographic Reach	Multiple continents/international
Policy Influence Type	Influenced training of practitioners or researchers
Impact	A review of the microscopy training courses available globally has been undertaken. We are in the process of collating and preparing a gap analysis. This will be used to guide RMS policy on training provided in the UK, both UK and international delegates.

Description	Training MSc students
Geographic Reach	Local/Municipal/Regional
Policy Influence Type	Influenced training of practitioners or researchers
Impact	Delivered a new training session to MSc students teaching viral quantification assays. This lead to a greater understanding of laboratory techniques and academic research.

Description	WHO R&D Blueprint Roadmaps: Consultation on Nipah virus
Geographic Reach	Multiple continents/international
Policy Influence Type	Participation in a advisory committee

Description	A One Health approach to pan-valent morbillivirus vaccines
Amount	£773,237 (GBP)
Funding ID	BB/R019843/1
Organisation	Biotechnology and Biological Sciences Research Council (BBSRC)
Sector	Public
Country	United Kingdom
Start	05/2018
End	03/2021

Description	Active and passive Immunity induced by aerosols
Amount	£100,000 (GBP)
Funding ID	BB/R506448/1
Organisation	Biotechnology and Biological Sciences Research Council (BBSRC)
Sector	Public
Country	United Kingdom
Start	10/2017
End	08/2021

Description	BBSRC - responsive mode
Amount	£187,370 (GBP)
Funding ID	BB/S007911/1
Organisation	Biotechnology and Biological Sciences Research Council (BBSRC)
Sector	Public
Country	United Kingdom
Start	03/2019
End	03/2022

Description	BBSRC Newton Fund Swine and Poultry Research Initiative
Amount	£865,623 (GBP)
Funding ID	BB/R01275X/1
Organisation	Biotechnology and Biological Sciences Research Council (BBSRC)
Sector	Public
Country	United Kingdom
Start	04/2018
End	06/2021

Description	BBSRC-iCASE
Amount	£100,000 (GBP)
Organisation	Biotechnology and Biological Sciences Research Council (BBSRC)
Sector	Public
Country	United Kingdom
Start	10/2019
End	09/2023

Description	British Egg Marketing Board PhD studentship
Amount	£76,000 (GBP)
Organisation	British Egg Marketing Board
Sector	Private
Country	United Kingdom
Start	10/2018
End	09/2022

Description	Components of the Medea gene drive system in a mosquito Aedes aegypti
Amount	£1,209,348 (GBP)
Funding ID	BB/S001964/1
Organisation	Biotechnology and Biological Sciences Research Council (BBSRC)
Sector	Public
Country	United Kingdom
Start	11/2018
End	10/2021

Description	DARPA PREEMPT
Amount	$2,700,000 (USD)
Organisation	Defense Advanced Research Projects Agency (DARPA)
Sector	Public
Country	United States
Start	10/2018
End	03/2022

Description	Development of novel universal influenza vaccines
Amount	£100,000 (GBP)
Organisation	Biotechnology and Biological Sciences Research Council (BBSRC)
Sector	Public
Country	United Kingdom
Start	10/2016
End	10/2020

Description	Dissecting the molecular pathways of MDV oncoprotein Meq for understanding pathogenesis and aid vaccine development
Amount	£440,000 (GBP)
Funding ID	BB/R007896/1
Organisation	Biotechnology and Biological Sciences Research Council (BBSRC)
Sector	Public
Country	United Kingdom
Start	04/2018
End	03/2021

Description	FLI- TPI joint PhD studentships Avian influenza immunology markers.
Amount	£100,000 (GBP)
Organisation	Friedrich Loeffler Institute
Sector	Public
Country	Germany
Start	07/2018
End	06/2022

Description	Further development of infectious bronchitis virus vaccines adapted for production in cell culture
Amount	£262,701 (GBP)
Funding ID	BB/R019576/1
Organisation	Biotechnology and Biological Sciences Research Council (BBSRC)
Sector	Public
Country	United Kingdom
Start	10/2018
End	03/2020

Description	Genomia Project Grant
Amount	£174,052 (GBP)
Organisation	Genomia fund
Sector	Charity/Non Profit
Country	United Kingdom
Start	02/2019
End	01/2020

Description	Identification and evaluation of swine mAbs in pig influenza challenge model
Amount	$599,944 (USD)
Funding ID	OPP1201470
Organisation	Bill and Melinda Gates Foundation
Sector	Charity/Non Profit
Country	United States
Start	10/2018
End	10/2019

Description	LVIF
Amount	$1,500,000 (CAD)
Organisation	Bill and Melinda Gates Foundation
Sector	Charity/Non Profit
Country	United States
Start	04/2018
End	09/2020

Description	NC3Rs Research Project Grant
Amount	£436,339 (GBP)
Funding ID	NC/R001138/1
Organisation	National Centre for the Replacement, Refinement and Reduction of Animals in Research (NC3Rs)
Sector	Private
Country	United Kingdom
Start	12/2017
End	11/2019

Description	Newton Fund: UK-China-Philippines-Thailand Swine and Poultry Research Initiative
Amount	£841,995 (GBP)
Funding ID	BB/R012679/1
Organisation	Biotechnology and Biological Sciences Research Council (BBSRC)
Sector	Public
Country	United Kingdom
Start	01/2018
End	12/2020

Description	Pathfinder BB/R012431/1 Grant holder: Dr Mark Fife. Grant title: chIFITM knockdown/knockout technology as a platform technology for increased vaccine yields in SPF Eggs.
Amount	£12,500 (GBP)
Funding ID	BB/R012431/1
Organisation	Biotechnology and Biological Sciences Research Council (BBSRC)
Sector	Public
Country	United Kingdom
Start	11/2017
End	02/2018

Description	Reducing the Economic and Zoonotic Impact of Avian Influenza (REZIAI): delivering novel vaccines and diagnostics from laboratory to the field.
Amount	£400,000 (GBP)
Funding ID	BB/S013792/1
Organisation	The Pirbright Institute
Sector	Academic/University
Country	United Kingdom
Start	02/2019
End	01/2021

Description	Seed Catalyst Award
Amount	£25,527 (GBP)
Funding ID	ISCF-TFPSA-Pirbright
Organisation	The Pirbright Institute
Sector	Academic/University
Country	United Kingdom
Start	11/2018
End	02/2019

Description	Taiwan Partnering Award
Amount	£40,700 (GBP)
Funding ID	BB/S020624/1
Organisation	Biotechnology and Biological Sciences Research Council (BBSRC)
Sector	Public
Country	United Kingdom
Start

Description	The Global Challenges Research Fund
Amount	£640,473 (GBP)
Organisation	Research Councils UK (RCUK)
Sector	Public
Country	United Kingdom
Start	04/2019
End	03/2024

Description	The impact of natural genetic variation on ruminant immune responses
Amount	£80,000 (GBP)
Organisation	University of Southampton
Sector	Academic/University
Country	United Kingdom
Start	10/2018
End	09/2022

Description	UK-China partnership on Global Food Security: Combating avian tumor diseases for sustainable poultry proudction
Amount	£382,000 (GBP)
Funding ID	BB/R007632/1
Organisation	Biotechnology and Biological Sciences Research Council (BBSRC)
Sector	Public
Country	United Kingdom
Start	04/2018
End	03/2020

Title	An ex vivo IBDV infection model using a chicken primary B cell culture system
Description	Recently, it has become possible to culture chicken primary B cells in vitro in the presence of a soluble construct of chicken CD40L that was made at The Pirbright Institute. We have demonstrated that these chicken primary B cells can be infected with infectious bursal disease virus (IBDV). This system will be validated and expanded to other B cell tropic viruses in order to replace the use of infected chickens in research, in an NC3Rs funded grant.
Type Of Material	Model of mechanisms or symptoms - in vitro
Year Produced	2017
Provided To Others?	Yes
Impact	Using this system, we have determined that chicken primary B cells respond to IBDV infection by inducing Type I IFN responses. However, the induction is more pronounced in cells infected with an attenuated strain compared to a very virulent strain. This is consistent with the very virulent strain down-regulating antiviral responses to a greater extent than other strains which may, in part, explain its enhanced virulence. We also observed a reduction in the expression of key genes involved in B cell proliferation and activation following IBDV infection which was only possible by using the primary B cell culture system.
URL	https://www.ncbi.nlm.nih.gov/pubmed/?term=broadbent+dulwich

Title	CRISPR/Cas9-based gene editing of avian herpesvirus vectors to develop novel recombinant vaccines
Description	Development of recombinant herpesvirus vaccines are usually achieved by classical homologous recombination methods. The advent of new genome editing tools has enabled its application on avian herpesvirus vaccine strains to develop new recombinant vaccines potentially capable of expressing protective antigens from multiple pathogens
Type Of Material	Technology assay or reagent
Year Produced	2017
Provided To Others?	Yes
Impact	The major impact will be in the generation of novel vaccines that can simultaneously protect against multiple avian diseases
URL	http://www.poultryworld.net/Health/Articles/2016/12/Is-GM-technology-the-future-of-poultry-vaccines-...

Title	Cattle MHC genotyping
Description	Using the sequence data generated through the targeted pull down of MHC, we developed a full gene and more targeted PCR approach to genotype cattle for the MHC class I region. This has been applied to many hundreds of samples to enable us to select individuals for breeding as well as survey genetic diversity in beef and diary herds.
Type Of Material	Technology assay or reagent
Year Produced	2018
Provided To Others?	No
Impact	After publication which we anticipate in 2019, we will appy this method to targeted herds and are already attracting industry interest.

Title	Comprehensive analysis of genetic variants across immune loci in indigenous breeds.
Description	The interferon-induced transmembrane (IFITM) protein family comprises a class of restriction factors widely characterised in humans for their potent antiviral activity. Their biological activity is well documented in several animal species, but their genetic variation and biological mechanism is less well understood, particularly in avian species. Here we report the complete sequence of the domestic chicken Gallus gallus IFITM locus from a wide variety of chicken breeds to examine the detailed pattern of genetic variation of the locus on chromosome 5, including the flanking genes ATHL1 and B4GALNT4. We have generated chIFITM sequences from commercial breeds (supermarket-derived chicken breasts), ancient bone samples, indigenous chickens from Nigeria (Nsukka) and Ethiopia, European breeds and inbred chicken lines from The Pirbright Institute, totalling of 211 chickens. Through mapping of genetic variants to the latest chIFITM consensus sequence our data reveal that the chIFITM locus does not show structural variation in the locus across the populations analysed, despite spanning diverse breeds from different geographic locations. However, single nucleotide variants (SNVs) in functionally important regions of the proteins within certain groups of chickens were detected, in particular the European breeds and indigenous birds from Ethiopia and Nigeria. In addition, we also found that two out of four SNVs located in the chIFITM1 (Ser36 and Arg77) and chIFITM3 (Val103) proteins were simultaneously under positive selection. Together these data suggest that IFITM genetic variation may contribute to the capacities of different chicken populations to resist viral infection.
Type Of Material	Model of mechanisms or symptoms - non-mammalian in vivo
Year Produced	2019
Provided To Others?	Yes
Impact	This paper is currently in press in. BMC Genomics.

Title	Detection of HA antigens of avian influenza viruses
Description	Assay for detection of recombinant HVT expressing HA antigens of avian influenza viruses
Type Of Material	Technology assay or reagent
Year Produced	2018
Provided To Others?	Yes
Impact	This new method will help to detect recombinant HVT vaccine expressing HA antigen in infected cell.

Title	Developed an in vivo infection model to generate highly pathogenic bovine RSV for challenge studies. Developed an accompanying in vitro titration method to calculate yield of virus produced.
Description	Developed an in vivo infection model to generate highly pathogenic bovine RSV for challenge studies. Developed an accompanying in vitro titration method to calculate yield of virus produced.
Type Of Material	Technology assay or reagent
Provided To Others?	No
Impact	Will help use and improve the calf challenge model of bovine RSV for vaccine testing.

Title	Development of assays for quantifying fusion inhibitory antibodies in sera (mFIT assay)
Description	We have developed a micro-fusion inhibition test (mFIT) assay to identify and quantify antibody responses (to vaccines etc.) which are capable of inhibiting viral induced cell-cell fusion. This test has been developed for various negative strand viruses.
Type Of Material	Technology assay or reagent
Year Produced	2018
Provided To Others?	No
Impact	This assay has allowed us to provide a more detailed examination of neutralising antibody responses to vaccines and when published will be disseminated to the field.

Title	Development of high-throughput assay for examining paramyxovirus fusion
Description	We have developed and optimised high-throughput assays (96 well plate format) to examine paramyxovirus fusion, using an adapted Bi-FC approach. We are using this to identify inhibitors of paramyxovirus, e.g. measles and RSV, fusion and attachment proteins. This has been integrated with next-generation platforms such as the Incucyte real-time imaging system available at The Pirbright Institute.
Type Of Material	Technology assay or reagent
Year Produced	2018
Provided To Others?	No
Impact	We are using this assay to examine the innate immune restriction of paramyxovirus replication.

Title	Development of tetramers to study immune responses in pigs
Description	The pig is a natural and important host of influenza viruses and is physiologically more comparable to humans than other animal models in terms of size, respiratory tract biology and volume. It is also an important vector in the birds to human infection cycle. A major drawback of the current pig model is the inability to analyze antigen-specific CD8+ T-cell responses, which are critical to respiratory immunity. We addressed this knowledge gap using an established in-bred pig model with a high degree of genetic identity between individuals, including the MHC (Swine Leukocyte Antigen (SLA)) locus. We developed a toolset that included long-term in vitro pig T-cell culture and cloning and identification of novel immunodominant influenza-derived T-cell epitopes. We also generated structures of the two SLA class I molecules found in these animals presenting the immunodominant epitopes. These structures allowed definition of the primary anchor points for epitopes in the SLA binding groove and established SLA binding motifs that were used to successfully predict other influenza-derived peptide sequences capable of stimulating T-cells.
Type Of Material	Technology assay or reagent
Year Produced	2017
Provided To Others?	No
Impact	Peptide-SLA tetramers were constructed and used to track influenza-specific T-cells ex vivo in blood, the lungs and draining lymph nodes. Aerosol immunization with attenuated single cycle influenza viruses (S-FLU) induced large numbers of CD8+ T-cells specific for conserved NP peptides in the respiratory tract. Collectively, these data substantially increase the utility of pigs as an effective model for studying protective local cellular immunity against respiratory pathogens.

Title	Established a method to immortalize bovine antigen presenting cells
Description	Established protocols to prepare lentiviruses expressing the proto-oncogenes Bmi1 and hTERT. Established protocols to transduce and generate immortal bovine antigen presenting cells derived from peripheral blood monocytes.
Type Of Material	Cell line
Provided To Others?	No
Impact	This is the first successful attempt at generating bovine immortal monocytic cell lines. This method can also be applied to immortalize B cells. This will result in reducing the number of animals used in experimentation and considerably reduce costs.

Title	Established human T cell lines expressing bovine WC1 genes for the analysis of WC1 signal transduction pathways.
Description	Established human Jurkat T cells expressing bovine the bovine gammadelta WC1 co-receptor
Type Of Material	Cell line
Provided To Others?	No
Impact	This cell line will help determine the function of the WC1 co-receptor in gammadelta T cell activation and function.

Title	Generated a MHC-defined bovine monophagocytic cell line.
Description	Using the method described above, successfully generated one MHC-defined bovine monophagocytic cell line (A18) which expresses and single classical MHC gene (HD6).
Type Of Material	Cell line
Provided To Others?	No
Impact	Will help reduce the number of animals used in experimentation. Will help resolve antigen presentation pathways in bovine cells and using bovine pathogens. Will help understand T cell activation pathways using MHC matched and mis-matched cells. Will help investigate bovine NK cell activation.

Title	Generated monophagocytic reporter cell lines to study the NF-kB signaling pathway
Description	Generated human monophagocytic cells expressing alkaline phosphatase reporter gene under a NF-kB promoter sequence.
Type Of Material	Cell line
Provided To Others?	No
Impact	This cell line is helping determine the role of RSV genes in immune modulation of monophagocytic cells in response to RSV infection.

Title	IBDV Reverse Genetics System
Description	We have developed a reverse genetics system for IBDV.
Type Of Material	Technology assay or reagent
Provided To Others?	No
Impact	We have used the reverse genetics system to generate recombinant IBDVs and chimeric recombinant IBDVs that contain genes from classical and very virulent strains of IBDV in the background of a cell culture adapted attenuated strain. This will enable us to study the effect of individual virus genes on pathogenicity.

Title	Optimisation of chicken kidney cell culture preparation
Description	We have optimised the method of extracting kidneys and preparing chicken kidney (CK) cell cultures from chickens. CK cells are used for in vitro assays to study avian viruses, in particular infectious bronchitis virus.
Type Of Material	Cell line
Year Produced	2017
Provided To Others?	No
Impact	This has increased the yield of viable cells and the quality of the cell cultures. We can now obtain the number of cells we require using fewer chicken kidneys, therefore requiring fewer chickens to be culled. This is an important development for the 3Rs. We hope to publish this method for others' information.

Title	Optimisation of preparation of TOCs from adult chickens
Description	Tracheal organ cultures (TOCs) are prepared from chicken embryos and used to assess growth of avian viruses that are not able to be propagated in cell culture. We have optimised the method of TOCs preparation from 3-week old chickens and are using these to assess viral genetic stability and phenotype in organ cultures that are a similar environment to the site of replication in whole chickens.
Type Of Material	Cell line
Year Produced	2018
Provided To Others?	No
Impact	We hope that this technique will reduce the number of chickens required for in vivo experiments, in line with the 3Rs, and plan to publish the method in due course.

Title	Stable chicken cell lines overexpressing chicken IFITM1,2 and 3
Description	In collaboration with Mark Fife, we have produced chicken stable cell lines overexpressing chicken IFITM1,2, 3 and a mutant chicken IFITM3 lacking palmitoylation sites.
Type Of Material	Technology assay or reagent
Year Produced	2017
Provided To Others?	No
Impact	These cell lines will be used to evaluate the effect of the IFITM proteins on the replication of different viruses, including IBDV.

Title	UniMMap- a pipeline for mapping RNAseq data over repetitive immune complexes.
Description	To exploit the abundance of available short read sequencing data we have developed a pipeline that uses mappability to accurately measure transcription over repetitive gene complexes. This methods uses known haplotypes to examine regions of uniqueness, and then the RNAseq data from the individual to train the method to be species or individual specific. This is particularly important over gene complexes that contain genes involved in the immune system, that are often highly similar in sequence but can have profoundly different functions.
Type Of Material	Technology assay or reagent
Year Produced	2018
Provided To Others?	No
Impact	the immediate impact will be adding fine resolution data to livestock gene expression atlas projects lead by the FAANG consortium. Further impact will be arising as this method is published and we apply it to numerous existing datasets the are publically avaialable as well as generated at Pirbright and by our collaborators.

Title	chimeric IBDVs
Description	Andrew Broadbent has engineered chimeric IBDVs expressing individual genes from a very virulent strain in the backbone of an attenuated strain.
Type Of Material	Technology assay or reagent
Year Produced	2018
Provided To Others?	No
Impact	These chimeric strains can be used to define viral determinants of virulence.

Title	method for identification of lung Tissue resident memory cells in pigs
Description	Recent overwhelming evidence indicates the importance of local tissue-resident memory T cells (TRM) in protective immunity. Most work on TRM has been performed in mice and the TRM defined as inaccessible to intravenously administered anti-T cell antibody. However there are very few data on TRM in large animals. For the first time we have defined TRM in the pig influenza model.
Type Of Material	Technology assay or reagent
Year Produced	2018
Provided To Others?	No
Impact	The identification of TRM and their robust protective capacity in site-specific infection has provided a new paradigm by which to assess T cell-mediated responses and an important new target for vaccine design. Since swine are an economically important species, are used as a large animal model for human infection and play a key role in the emergence of novel and potentially zoonotic influenza viruses, the identification of TRM in pigs will allow us to study their role in immunity to swine influenza.and how best to induce them by immunisation.

Title	replication competent recombinant split-GFP infectious bursal disease virus
Description	We have generated a replication competent recombinant split-GFP infectious bursal disease virus (IBDV) where a small GFP11 molecule is tagged to the virus polymerase (VP1) to make IBDV-VP1-GFP11. Infected cells that express the GFP1-10 molecule fluoresce green when the GFP11 tag complements the GFP1-10. The IBDV-VP1-GFP11 colocalises with VP3 and dsRNA and therefore is likely to be present at the sites of virus replication.
Type Of Material	Technology assay or reagent
Year Produced	2017
Provided To Others?	No
Impact	This tool can be used to study IBDV replication complex development in infected cells.

Title	tetracysteine-tagged IBDV
Description	Andrew Broadbent has engineered a tetracysteine (TC)-tagged IBDV that can be used in live cell imaging and co-infection studies with the split-GFP IBDV.
Type Of Material	Technology assay or reagent
Year Produced	2018
Provided To Others?	No
Impact	This tool, combined with the split-GFP IBDV, will enable us to study co-infection, super-infection exclusion, and the intracellular requirements for viral reassortment.

Title	Analysis of paramyxovirus replication using Incucyte real-time imager
Description	We have optimised a data analysis pipeline for the Incucyte real-time imager to allow us to track viral replication in a high-throughput fashion (up to 6 x 96-well plates). This is based on simultaneous analysis of phase, as well as green and red fluorescence.
Type Of Material	Data analysis technique
Year Produced	2018
Provided To Others?	No
Impact	Only preliminary data to date.

Title	E-MTAB-5947- Transcription profiling by array of differential gene expression in chicken primary B cells infected ex vivo with attenuated and very virulent strains of infectious bursal disease virus (IBDV)
Description	Microarray data submitted to ArrayExpress from chicken primary B cells infected with a very virulent and attenuated strain of IBDV.
Type Of Material	Database/Collection of data
Year Produced	2017
Provided To Others?	Yes
Impact	This is the first ever microarray data of chicken primary B cells infected with IBDV and will be of use to other researchers in the area of B cell biology or IBDV virology
URL	https://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-5947/

Description	Aerosol delivery of vaccines and therapeutics
Organisation	Aerogen
Country	Ireland
Sector	Private
PI Contribution	Developed the pig influenza model which is a natural host pathogen system
Collaborator Contribution	Provided expertise and equipment for aerosol delivery
Impact	Successfully delivered vaccines by aerosol
Start Year	2015

Description	Andrew Davidson - Proteome of viral replication organelles
Organisation	University of Bristol
Department	Avon Longitudinal Study of Parents and Children (ALSPAC)
Country	United Kingdom
Sector	Academic/University
PI Contribution	Projects are being initiated to study the cellular proteins associated with coronavirus replication organelle membranes and a funding application has been submitted to BBSRC.
Collaborator Contribution	Andrew has provided assistance with experimental planning and drafting of a grant application. He has also provided protocols to allow preliminary data to be generated. Once protocols have been established, Andrew will facilitate mass spectrometry data generation and will analyse the resulting data.
Impact	No outcomes to date, projects being initiated and funding applications in progress.
Start Year	2018

Description	Annika Posautz; University of Veterinary Medicine Vienna. Morbillivirus host-diversity analysis.
Organisation	University of Veterinary Medicine Vienna
Country	Austria
Sector	Academic/University
PI Contribution	Sharing of research data regarding morbillivirus host-range and technical support to the project.
Collaborator Contribution	Provision of tissue samples from potential morbillivirus hosts, e.g. carnivores.
Impact	No outcomes as yet.
Start Year	2018

Description	BBSRC-UC Davis workshop, 16-18 October
Organisation	University of California, Davis
Department	UC Davis College of Biological Sciences
Country	United States
Sector	Academic/University
PI Contribution	Presented on "Reproducibility and relevance in vector infection studies". Followed up with discussion of potential publication and funding opportunities.
Collaborator Contribution	Various presentations on vectors and vector-borne diseases including, facilities, challenges, opportunities.
Impact	Discussion of potential joint publications and grant applications; no tangible impacts yet.
Start Year	2017

Description	BBSRC-UC Davis workshop, 16-18 October
Organisation	University of Liverpool
Country	United Kingdom
Sector	Academic/University
PI Contribution	Presented on "Reproducibility and relevance in vector infection studies". Followed up with discussion of potential publication and funding opportunities.
Collaborator Contribution	Various presentations on vectors and vector-borne diseases including, facilities, challenges, opportunities.
Impact	Discussion of potential joint publications and grant applications; no tangible impacts yet.
Start Year	2017

Description	Bridget Penman
Organisation	University of Warwick
Department	School of Life Sciences
Country	United Kingdom
Sector	Academic/University
PI Contribution	We are providing genetic data and diversity measures of MHC and associated receptors to inform the modelling efforts to elucidate which selection pressures are the main drivers for the types of diversity we see in extant species.
Collaborator Contribution	They are experts in mathmatical modelling and will provide data to help explain genetic diversity in cattle.
Impact	Not yet
Start Year	2017

Description	Challenge of transgenic chickens with influenza viruses
Organisation	Imperial College London
Department	Section of Virology
Country	United Kingdom
Sector	Academic/University
PI Contribution	We have challenged transgenic chickens with recombinant avian influenza viruses to determine outcome; infectivity, transmission, pathogenicity, dissemination within host. We also processed the samples generated from these animal experiments at The Pirbright Institute.
Collaborator Contribution	Imperial College (Wendy Barclay and Mike Skinner) generated recombinant avian influenza viruses for challenge.
Impact	This collaboration has resulted in the successful award of a joint grant with imperial college aiming to carry out challenge experiments on novel transgenic chickens to facilitate control of avian influenza virus in the poultry population. BB/S007911/1 - Investigating the role of ANP32A in the replication of avian Influenza virus
Start Year	2018

Description	Charles Sande - KEMRI - Kenya: RSV collaboration
Organisation	Kenyan Institute for Medical Research (KEMRI)
Country	Kenya
Sector	Public
PI Contribution	Development of research proposal for PhD studentship on the innate immune response during RSV infection. This student will be hosted by my lab for 18 months from 2020 onwards.
Collaborator Contribution	Project design. Application for PhD funding and provision of technical support.
Impact	The studentship was awarded to Dr. Sande in Kenya and the student has been recruited. They will commence their studies in Spring 2019 and come to the UK in early 2020.
Start Year	2018

Description	Collaboration on replication of mumps virus in cells
Organisation	University of Copenhagen
Country	Denmark
Sector	Academic/University
PI Contribution	Intellectual input so far, training research assistant to prepare samples, imaging samples in transmission electron microscopes
Collaborator Contribution	Preliminary confocal microscopy to optimize sample conditions. Preparation of samples for EM.
Impact	No outcomes yet
Start Year	2018

Description	Collaboration with University of Copenhagen CFIM on cell biology of virus-host interactions
Organisation	University of Copenhagen
Country	Denmark
Sector	Academic/University
PI Contribution	Intellectual input into virus projects which come in to the microscopy core facility at the University. Limited practical work and training given to researchers there.
Collaborator Contribution	Research projects are presented to the core facility, I advise on the best route for achieving the data required and those researchers complete preliminary data following my advice. Collaborators are then able to produce the best possible samples for further advanced microscopy as necessary.
Impact	No tangible outcomes yet as we are in early stages. This is multi-disciplinary as projects can come from several University departments (medical, pathology, cell biology, veterinary, food), and other Universities in Denmark (DTU).
Start Year	2018

Description	Components of the Medea gene drive system in a mosquito Aedes aegypti
Organisation	National Health Research Institutes (NHRI) Taiwan
Department	Institute of Molecular and Genomic Medicine
Country	Taiwan, Province of China
Sector	Charity/Non Profit
PI Contribution	Developed joined research project and lead successful application for funding
Collaborator Contribution	Various lines of research, including construction of plasmids and development and characterisation of transgenic mosquito strains; and group discussions about results, conclusions and research progress.
Impact	No impact yet.
Start Year	2018

Description	Components of the Medea gene drive system in a mosquito Aedes aegypti
Organisation	National Institute of Genetics
Department	NIG-FLY, Mishima (Drosophila RNAi resources)
Country	Japan
Sector	Public
PI Contribution	Developed joined research project and lead successful application for funding
Collaborator Contribution	Various lines of research, including construction of plasmids and development and characterisation of transgenic mosquito strains; and group discussions about results, conclusions and research progress.
Impact	No impact yet.
Start Year	2018

Description	David Hughes - University of St. Andrews. RSV preparation.
Organisation	St. Andrews University
PI Contribution	Sharing of preliminary research data on innate immune restriction of RSV.
Collaborator Contribution	Provision of cell-lines (Hep2-Npro) to support generation of high titre stocks of RSV. Also associated protocols.
Impact	No outcomes as yet, beyond preliminary data.
Start Year	2018

Description	David Matthews - Proteomics informed by transcriptomics
Organisation	University of Bristol
Department	Proteomics Facility
Country	United Kingdom
Sector	Academic/University
PI Contribution	Projects are being initiated to study the cellular proteins associated with coronavirus replication organelle membranes and a funding application has been submitted to BBSRC.
Collaborator Contribution	David has assisted in project design and drafting of a funding application to BBSRC. We are in the process of generating transcriptomics data, which David will process to provide an expressed protein database for peptide identification following mass spectrometry.
Impact	No outcomes to date, a funding application has been submitted to BBSRC.
Start Year	2018

Description	Derek Bickhart-USDA
Organisation	U.S. Department of Agriculture USDA
Department	Beltsville Agricultural Research Center
Country	United States
Sector	Academic/University
PI Contribution	Our group has provided the genomic framework of the cattle NK cell regions and the biological input.
Collaborator Contribution	The ARS group has used our data as a framework to map and predict cattle SNPs for variable immune traits using their whole genome data
Impact	Research application based on preliminary data to a BBSRC-NIFA call. This collaboration is multi-disciplinary combining molecular biology and computational biology
Start Year	2014

Description	Development of tetramers in the Babraham pig model
Organisation	Cardiff University
Country	United Kingdom
Sector	Academic/University
PI Contribution	Developed the aerosol delivery method of vaccine and provided material (spleen, BAL and blood) from inbred animals (Babraham pigs) following immunisation with the candidate universal vaccine, S-FLU.
Collaborator Contribution	Developed porcine influenza specific class I tetramers and provided the epitope map of Babrahams S-Flu responses to NP, M1, M2, PB1 and PB2 that could be presented by SLA-1 or SLA-2 Grew pig T cell clones for the first time in 30 years.
Impact	Multidisciplinary - immunology, virology, protein chemistry. Paper not yet published
Start Year	2014

Description	Dr Michael Skinner, Imperial College London
Organisation	Imperial College London
Department	Department of Primary Care and Public Health
Country	United Kingdom
Sector	Academic/University
PI Contribution	We aim to compare the host transcriptional response of primary chicken B cells infected with different strains of IBDV of varying virulence. We have the specialist reagents and expertise in house that are required to culture primary chicken B cells, and we have access to different strains of IBDV.
Collaborator Contribution	Dr Michael Skinner and his team at Imperial College London have the expertise and facilities to generate and analyse RNA-Seq data in order to determine host transcriptional responses. This is required in order to complete the aim of the project.
Impact	Further funding: PhD studentship (£102,800) - Dr Andrew Broadbent at The Pirbright Institute and Dr Michael Skinner at Imperial College London are joint supervisors for a PhD student working on this project. Further Funding: Houghton Trust (£8,000)- Dr Andrew Broadbent was awarded a Houghton Trust Small Research Grant to contribute to this project.
Start Year	2014

Description	Efficacy of a candidate universal influenza vaccine, S-FLU.
Organisation	University of Oxford
Department	Radcliffe Department of Medicine
Country	United Kingdom
Sector	Academic/University
PI Contribution	Tested the immunogenicity and efficacy of a candidate universal influenza vaccine, S-FLU, in pigs. For the first time we demonstrated that S-FLU when administered by aerosol can reduce viral load in nasal swabs and lung in pigs after influenza virus challenge. We have shown that the most efficient way to administer this vaccine is by aerosol.
Collaborator Contribution	Professor Alain Townsend has developed the S-FLU vaccine.
Impact	The most efficient way to induce immune response in the lung is after aerosol delivery of LAIV vaccines in pigs. The most efficient way to induce cross-protective immunity is by aerosol delivery of S-FLU to the lungs of pigs
Start Year	2014

Description	Engineering mosquitoes for reduced vector competence for chikungunya virus
Organisation	University of Malaya
Department	Department of Medical Microbiology
PI Contribution	Developed joint research programme, led successful application for funding, coordinate programme.
Collaborator Contribution	Various specific strands of research, and group discussions about results, conclusions, research progress and future activities.
Impact	reported under funding
Start Year	2016

Description	Engineering mosquitoes for reduced vector competence for chikungunya virus
Organisation	University of Melbourne
Country	Australia
Sector	Academic/University
PI Contribution	Developed joint research programme, led successful application for funding, coordinate programme.
Collaborator Contribution	Various specific strands of research, and group discussions about results, conclusions, research progress and future activities.
Impact	reported under funding
Start Year	2016

Description	Engineering mosquitoes for reduced vector competence for chikungunya virus
Organisation	University of Tartu
Department	Institute of Technology
PI Contribution	Developed joint research programme, led successful application for funding, coordinate programme.
Collaborator Contribution	Various specific strands of research, and group discussions about results, conclusions, research progress and future activities.
Impact	reported under funding
Start Year	2016

Description	Factor H and influenza virus
Organisation	Brunel University London
Department	Brunel Arts
Country	United Kingdom
Sector	Academic/University
PI Contribution	Our research team are hosting a PhD student from Brunel University to facilitate the investigation of any interaction between influenza virus and complement factor H. We are providing reagents, expertise in handling influenza virus and advice on the scientific hypothesis of the research programme.
Collaborator Contribution	Brunel university initiated the project and provided reagents including antibodies and methods for purification human co-factor H protein. They also provide funding for this PhD studentship.
Impact	No outcomes as yet
Start Year	2018

Description	Gene editing chickens with Roslin
Organisation	University of Edinburgh
Department	Roslin Institute Transgenic Chicken Facility
Country	United Kingdom
Sector	Academic/University
PI Contribution	We have provided infectious pathogen challenge and infectious pathogen sample processing facilities in this collaboration. We infected the transgenic chickens with avian influenza virus and processed the samples following challenge.
Collaborator Contribution	Helen Sang and Mike McGrew at The Roslin Institute (University of Edinburgh) contributed by producing and providing transgenic chickens (Split GFP) for challenge. Expertise in genotype screening the transgenic birds was shared with us.
Impact	We have now successfully received a BBSRC responsive mode grant to carry out more transgenic bird challenges with influenza viruses. BB/S007911/1 -Investigating the role of ANP32A in the replication of avian Influenza virus.
Start Year	2018

Description	Generation of immortalised bovine antigen presenting cells
Organisation	University of Surrey
Country	United Kingdom
Sector	Academic/University
PI Contribution	Using a lentivirus vector expressing proto-oncogenic genes, we generated MHC-defined immortal bovine antigen presenting cells that can be used in antigen presentation studies or virus-host interaction studies in the future.
Collaborator Contribution	The University of Surrey provided several lenvirus vectors and preparation for immortalization studies.
Impact	A publication in progress titled "Generation of immortal bovine antigen presenting cells" and the generation of a library of MHC-defined bovine cell lines.
Start Year	2017

Description	Horizon Discovery Ltd Cambridge Research Park, United Kingdom
Organisation	Horizon Discovery Group plc
PI Contribution	Verify IFITM knock-out cell by qPCR and immuno-blotting. Verification of further knock-out cell lines by DNA sequencing, immuno-blotting and qPCR Cell infection with Avian Influenza Viruses Assess the viral titre of cells infected with Influenza Viruses in K/O and wildtype cells. Quantitative analysis of viral infection in IFITM K/O cells Demonstrate increased AIV viral titres at lab scale and estimation of what this may equate to with respect to vaccine dose yields. Analysis of IFITM K/O cell lines permissivity to a range of additional animal viruses (Relevant to the LVIF). Milestone 7: Demonstrate permissivity increased and viral titres for a range of LVIF relevant viruses in the edited cell lines.
Collaborator Contribution	Develop a CRISPR/cas9 gene editing system to specifically target the chIFITM locus in avian cell culture
Impact	Grant funding
Start Year	2017

Description	ILRI Research Collaboration (Kenya, March 2017)
Organisation	International Livestock Research Institute (ILRI)
Country	Kenya
Sector	Charity/Non Profit
PI Contribution	Exchange of ideas and samples for indigenous breeds of livestock
Collaborator Contribution	Contributed samples for analysis.
Impact	Analysis of indigenous breed genomic sequences for Immune function genes.
Start Year	2017

Description	Identification of conserved B-cell epitopes of highly pathogenic coronaviruses for broadspectrum immunotherapy and vaccine design.
Organisation	Imperial College London
Country	United Kingdom
Sector	Academic/University
PI Contribution	We have performed experiments to generate preliminary data for a collaborative grant application, contributed to the writing of the grant proposal and responded to reviewers comments.
Collaborator Contribution	My collaborators have performed experiments to generate preliminary data for a collaborative grant application, contributed to the writing of the grant proposal and responded to reviewers comments.
Impact	Submission of proposal entitled "Identification of conserved B-cell epitopes of highly pathogenic coronaviruses for broadspectrum immunotherapy and vaccine design" to the "One Health Approaches to Accelerate Vaccine Development" call that forms part of the UK government's commitment to Official Development Assistance (ODA) in October 2017.
Start Year	2017

Description	Identification of conserved B-cell epitopes of highly pathogenic coronaviruses for broadspectrum immunotherapy and vaccine design.
Organisation	University of Kent
Country	United Kingdom
Sector	Academic/University
PI Contribution	We have performed experiments to generate preliminary data for a collaborative grant application, contributed to the writing of the grant proposal and responded to reviewers comments.
Collaborator Contribution	My collaborators have performed experiments to generate preliminary data for a collaborative grant application, contributed to the writing of the grant proposal and responded to reviewers comments.
Impact	Submission of proposal entitled "Identification of conserved B-cell epitopes of highly pathogenic coronaviruses for broadspectrum immunotherapy and vaccine design" to the "One Health Approaches to Accelerate Vaccine Development" call that forms part of the UK government's commitment to Official Development Assistance (ODA) in October 2017.
Start Year	2017

Description	Identification of human orthologues of bovine Workshop Cluster 1 genes
Organisation	University of Oxford
Department	Nuffield Department of Medicine
Country	United Kingdom
Sector	Academic/University
PI Contribution	The Workshop Cluster 1 (WC1) gene family encodes a group of proteins thought to be involved in immune recognition. These genes are exclusively expressed on T cells expressing the gamma and delta T cell receptor and are well characterized in the bovine system. We have began investigating the presence and expression of WC1 on human cells using co-immunoprecipitation and mass spectrometry analysis of putatitve human WC1 genes.
Collaborator Contribution	The Univ. of Oxford has developed a pipeline to identify protein fragments using mass spectrometry and bioinformatics from putative proteins from which little or no information is currently known. This pipeline has been applied to identify putative human WC1 proteins obtained from co-immunoprecipitation/mass spectrometry studies.
Impact	The identification of one human WC1 gene/protein. A publication in progress titled "Identification of the human orthologue of a Workshop Cluster 1 gene expressed on gammadelta T cells"
Start Year	2017

Description	Imperial
Organisation	Imperial College London
Country	United Kingdom
Sector	Academic/University
PI Contribution	Joint supervision of PhD student
Collaborator Contribution	Joint supervision of PhD student
Impact	One successful completed PhD student. Papers published or in preparation. Another PhD student in progress.
Start Year	2010

Description	Influenza and co-factor H protein
Organisation	Leibniz Association
Department	Leibniz Institute for Natural Product Research and Infection Biology
Country	Germany
Sector	Public
PI Contribution	We are screening influenza virus interaction with co-factor H proteins to determine the interaction interface.
Collaborator Contribution	Christine Skerka has provided fragment factor H peptides to facilitate our mapping studies of the interaction surface with influenza viruses.
Impact	No outcomes as yet
Start Year	2019

Description	Investigating antigenic determinants inducing stronger and broader cross-protective immunity among H5 avian influenza viruses
Organisation	Royal Veterinary College (RVC)
Department	Veterinary Basic Sciences
Country	United Kingdom
Sector	Academic/University
PI Contribution	The overarching aims of the proposed research are to improve controls against H5 subtype of avian influenza viruses infecting poultry by investigating underlying mechanisms that define how vaccines renders effectiveness and develop novel approaches enhancing the effectiveness of H5 avian influenza vaccines. We recently generated a panel of monoclonal antibodies (mAbs) that recognise a variety of different clades of H5 viruses. Propagation of AIV in eggs or cultured cells together with mAbs drives virus to escape from mAbs neutralisation activity. These classical virus neutralization assays (VN) followed by HA gene sequence analysis allowed us to defined the role of amino acid substitutions that are associated with evasion of antibody neutralization through emergence of antigenic variants and failure of vaccine efficacy.
Collaborator Contribution	provided intellectual input for achieving prescribed objectives of this work.
Impact	Talk entitled "Molecular determinants for antigenicity and vaccine efficacy of avian influenza viruses" was presented at Newton Agham Researcher Links Workshop " Novel Vaccines and Diagnostic Technologies against Emerging and re-emerging Veterinary Pathogens" at Rizal Park Hotel, Manila, Philippines, 4-7 February 2019.
Start Year	2019

Description	Investigating the role of the SH protein of RSV in the duration of immunity
Organisation	Imperial College London
Department	Department of Medicine
Country	United Kingdom
Sector	Academic/University
PI Contribution	We provided initial data supporting the hypothesis that the small hydrophobic protein of bovine respiratory syncytial virus regulates the host's immune response by modulating the transcription factors NF-kB and STAT1 in bovine cells
Collaborator Contribution	Based on our results, Imperial has began investigating the role of the small hydrophobic protein of human respiratory syncytial virus on human cells.
Impact	Publication in progress titled "Regulation of the B cell activating factor BAFF by the small hydrophobic protein of respiratory syncytial virus".
Start Year	2017

Description	Invitation to participate in the Vaccine Innovation Forum 2017 at Shanghai 26-27 April 2017
Organisation	Chinese Academy of Agricultural Sciences
Department	Shanghai Veterinary Research Institute
PI Contribution	Prof. Nair was invited to participate in the Vaccine Innovation Forum 2017 at Shanghai 26-27 April 2017 to present a talk on 'Novel Vaccine Development for Avian Virus Diseases'. He also participated in the round table discussions on the challenges and opportunities in the vaccines and vaccination sector
Collaborator Contribution	Vaccine Innovation Forum will help in expanding the future collaborations and activities of the UK-China Centre of Excellence for Research on Avian Diseases (http://www.uk-china-cerad.org/).
Impact	organised joint meeting in China and the UK to discuss on challenges and opportunities
Start Year	2017

Description	JN - Preparation of capped mesoporpus silica nanoparticles
Organisation	Polytechnic University of Valencia
Country	Spain
Sector	Academic/University
PI Contribution	We were involved with testing the nanoparticles once they had been made. We also contributed purified antibody and shipped it to our collaborators for the generation of one type of nanoparticle, which we subsequently tested. We were involved with the intellectual design of experiments
Collaborator Contribution	Our partners generated the nanoparticles and shipped them to us for testing. Our partners were also involved with the intellectual design of experiments.
Impact	Experimental work performed to investigate the use of mesoporous silica nanoparticles to detect FMDV proteases in clinical samples
Start Year	2016

Description	JS - Production of monoclonal antibodies that recognise FMDV VP4 from mouse spleens
Organisation	University of Edinburgh
Department	The Roslin Institute
Country	United Kingdom
Sector	Academic/University
PI Contribution	VLPs displaying the N-terminal 15 amino acids of FMDV were designed and produced at The Pirbright Institute and then mice were immunised with them. The spleens of the immunised mice were harvested and splenocytes stored. The response to the VP4 sequence was checked by ELISA and the mouse with the best response was selected to send the splenocytes for monoclonal production.
Collaborator Contribution	The collaborators took the splenocytes obtained from the mouse experiments and carried out the fusions. They then screened the supernatants for positive wells that detected the VP4 sequence.
Impact	Positive wells have been identified indicating some monoclonals that are specific for VP4 have been generated. These will be further screened for ability to recognise virus and to neutralise infection.
Start Year	2017

Description	Leeds FBS
Organisation	University of Leeds
Department	Leeds Institute of Molecular Medicine
Country	United Kingdom
Sector	Academic/University
PI Contribution	Research
Collaborator Contribution	Research and student supervision
Impact	Research
Start Year	2009

Description	Martin Ludlow - University of Veterinary Medicine, Hanover. Morbillivirus collaboration.
Organisation	University of Veterinary Medicine Hanover
PI Contribution	Provision of technical information and advice about the host-range of morbilliviruses.
Collaborator Contribution	Provision of reagents to support our research project, e.g. plasmid clones of viral attachment proteins and receptors.
Impact	No outcomes as yet, only preliminary data.
Start Year	2018

Description	Miguel Ángel Muñoz Alía - Mayo clinic, USA. Measles population diversity.
Organisation	Mayo Clinic
Country	United States
Sector	Charity/Non Profit
PI Contribution	Technical support and sharing of research data on the host-range of morbilliviruses.
Collaborator Contribution	Provision of plasmid clones representing the broad genetic diversity of measles attachment and fusion proteins. Technical support.
Impact	No outcomes as yet.
Start Year	2018

Description	Mosquito Interest Group
Organisation	Imperial College London
Country	United Kingdom
Sector	Academic/University
PI Contribution	Organising and attending meetings of students, RAs, postdocs and PIs on mosquito biology and related techniques.
Collaborator Contribution	Organising and attending meetings of students, RAs, postdocs and PIs on mosquito biology and related techniques.
Impact	Exchange of practical techniques, for instance in tracking oviposition of individual mosquitoes. Brainstorming for projects and papers, resulting in a GCRF VBD network proposal (which passed the first review but was not funded in the second) and an informal collaboration (which was intended for submission to UK-Thai call in 2017 but Thai partner was not eligible). Exchanges of colony strains of insects planned this month.
Start Year	2016

Description	Mucosally Associated Invariant T cells (MAIT) in pig influenza model
Organisation	University of Oxford
Country	United Kingdom
Sector	Academic/University
PI Contribution	Identified MAIT cells in pig Influenza model. Provide samples from infected and immunised protected animals
Collaborator Contribution	Provide expertise in characterising MAIT: development of tetramer; Zell scanner technology allowing high resolution studies of cell phenotype and functions
Impact	Joint PhD studentship with Paul Klenerman, University of Oxford
Start Year	2016

Description	Nicolas Locker - Investigation of translation regulation by infectious bronchitis virus
Organisation	University of Surrey
Country	United Kingdom
Sector	Academic/University
PI Contribution	My team provide experience in molecular virology and cellular interactions of infectious bronchitis virus including previous experience of IBV accessory proteins regulating cellular translation. A PhD student works jointly between our labs.
Collaborator Contribution	Dr Locker's team provide expertise in viral regulation of cellular translation and stress granule signalling. Dr Locker is also providing protocols, advice and training for techniques including ribopuromycylation and ribosome profiling. A PhD studentworks jointly between our labs.
Impact	PhD student funding (studentship commenced Oct 2017) - Control of host cell translation by infectious bronchitis virus
Start Year	2016

Description	Oxford - Strubi
Organisation	University of Oxford
Country	United Kingdom
Sector	Academic/University
PI Contribution	Collaboration on virus structural biology and joint PhD studentship
Collaborator Contribution	Collaboration on virus structural biology and joint PhD studentship
Impact	Collaboration on virus structural biology has produced a number of novel virus structures and new understanding of virus entry and packaging
Start Year	2008

Description	Oxford University Collaborations
Organisation	University of Oxford
Department	Big Data Institute
Country	United Kingdom
Sector	Academic/University
PI Contribution	Scientific discussion, collaboration and manuscript preparation
Collaborator Contribution	Scientific discussion, collaboration and manuscript preparation
Impact	Manuscript in preparation
Start Year	2017

Description	Parham Lab at Stanford
Organisation	Stanford University
Department	School of Medicine
Country	United States
Sector	Academic/University
PI Contribution	Our group designed the experiments and generated a majority of the data. We also led the analysis of the data and writing of the manuscripts
Collaborator Contribution	The Parham lab is the leading group worldwide in the evolutionary study of NK cell receptors and MHC class I. This group generated part of the data and helped in the analysis, interpretation and manuscript preparation
Impact	Definition of the Cattle Killer Cell Igndash like Receptor Gene Family: Comparison with Aurochs and Human Counterparts Nicholas D. Sanderson, Paul J. Norman, Lisbeth A. Guethlein, Shirley Ellis, Christina Williams, Matthew Breen, Steven D. E. Park, David A. Magee, Farbod Babrzadeh, Andrew Warry, Mick Watson, Daniel G. Bradley, David E. MacHugh, Peter Parham, and John A. Hammond. Journal of Immunology, Dec 15, 2014; 193 (12) This collaboration is not multi-didciplinary
Start Year	2009

Description	Partnering with National Taiwan University
Organisation	National Taiwan University
Country	Taiwan, Province of China
Sector	Academic/University
PI Contribution	We have prepared and submitted an application for a BBSRC-Taiwan Partnering Award to study "Assembly of Recombinant Infectious Bronchitis Virus and Determination of Antigenic Sites that Confer Hemagglutination Activity".
Collaborator Contribution	The partners conceived the project and got in contact about submitting an application for funding together.
Impact	An application has been made for a BBSRC-Taiwan Partnering Award in November 2018.
Start Year	2018

Description	Paul Digard - The Roslin Insititute
Organisation	University of Edinburgh
Department	The Roslin Institute
Country	United Kingdom
Sector	Academic/University
PI Contribution	We generated constructs that express PB1-F2 from a variety of avian influenza strains in eukaryotic expression plasmids. We generated Chimera PB1-F2 expression plasmids where the C-terminus of the PB1-F2 are switched between strains. Using these plasmids we have determined the localization and antagonist activity toward the IFN beta signaling pathway and NF-kb signaling pathway in human and chicken cells. We have therefore provided reagents and intellectual input to the collaboration.
Collaborator Contribution	Our collaborators have used these plasmids in there well defined stability assays to determine sequence specific effects on this characteristic. Intellectual input and expertise in a particular assay were made by these collaborators.
Impact	We have successfully published some of this work; "The cellular localization of avian influenza virus PB1-F2 protein alters the magnitude of IFN2 promoter and NF?B-dependent promoter antagonism in chicken cells." doi: 10.1099/jgv.0.001220. We have an ongoing joint PhD studentship in this area. This collaboration remains current and active.
Start Year	2017

Description	Paul Verkade - Electron tomography and CLEM
Organisation	University of Bristol
Department	Avon Longitudinal Study of Parents and Children (ALSPAC)
Country	United Kingdom
Sector	Academic/University
PI Contribution	Nicole Doyle has performed electron tomography to analyse the three dimensional structure of membrane rearrangements induced by the expression of IBV non-structural proteins 4 or 3 and 4 in the absence of other viral components. Sample set up was performed at Pirbright and analysis was subsequently performed at University of Bristol followed by additional analysis at Pirbright.
Collaborator Contribution	Paul has assisted with electron tomography data generation providing access to electron microscopes in Bristol as well as training for Nicole Doyle in performing experiments and analysing and modelling data. He is also advising Nicole while establishing correlative light electron microscopy at Pirbright.
Impact	doi: 10.3390/v10090477
Start Year	2018

Description	Professor Sahyan Sharif, University of Guelph, Canada
Organisation	University of Guelph
Department	Department of Pathobiology
Country	Canada
Sector	Academic/University
PI Contribution	Collaboration between my team and Professor Sharif lead to new finding in Avian immunology, specifically in understanding the effects of nutrients on antigen presenting cells and T cells of chicken. We took the lead in studying the effects of some nutrients (Vitamin D) on the function of chicken T cells, performed most of the experiments in our laboratory. Meanwhile, we participated in studying the effects of Vitamin D on antigen presenting cells of chicken by performing some key experiments because we had access to some specific reagents (monoclonal antibodies) recognizing molecules expressed on chicken antigen presenting cells.
Collaborator Contribution	Professor Sharif team took the lead in studying the effects of Vitamin D on antigen presenting cells of chicken, and contributed to experimental plan for studying the effects on chicken T cells.
Impact	Two publications in 2015 and 2016: 1. Nitish Boodhoo, Shayan Sharif, Shahriar Behboudi. 1a,25(OH)2 Vitamin D3 Modulates Avian T Lymphocyte Functions without Inducing CTL Unresponsiveness. PLoS One. 2016 Feb 24;11(2):e0150134 2. Bahram Shojadoost, Shahriar Behboudi, Villanueva AI, Jennifer Brisbin, Ali Ashkar, Shayan Sharif. Vitamin D3 modulates the function of chicken macrophages. Res. Vet. Sci. 2015 June, 100: 45-51
Start Year	2014

Description	Pseudoparticles of influenza for interaction studies with co-factor H
Organisation	University of Kent
Department	Medway School of Pharmacy
Country	United Kingdom
Sector	Hospitals
PI Contribution	We have screened the interaction between influenza pseudotype particles that only contain the surface HA protein from influenza viruses for interaction with human co-factor H protein. We have screen pseudotype particles of multiple influenza subtypes.
Collaborator Contribution	Nigel Temperton of the viral Pseudotype Unit at the School of Pharmacy provided influenza virus pseudotype particles of multiple subtypes to allow us to screen for interaction with co-factor H protein.
Impact	No outcomes yet
Start Year	2018

Description	Research Collaboration with Nigeria (23rd - 28th January 2017)
Organisation	University of Nigeria
Country	Nigeria
Sector	Academic/University
PI Contribution	Analysis of rare breed indigenous chicken in Nigeria.
Collaborator Contribution	Helped with sampling and background information of the breed.
Impact	Analysis is ongoing.
Start Year	2017

Description	Steve Goodbourn - St Georges - chicken innate molecule interactions.
Organisation	Ulster University
Department	Biomedical Sciences Research Centre
Country	United Kingdom
Sector	Academic/University
PI Contribution	We have collaborated by producing yeast- 2-hybrid plasmids containing the influenza PB1-F2 gene from various avian influenza strains. The PhD student on this studentship then visited Professor Goodbourn's laboratory to carry out the yeast-2-hybrid screen for interaction innate chicken molecules.
Collaborator Contribution	Professor Goodbourn's laboratory provided yeast-2-hybrid plasmids containing various innate chicken molecules to be screened for interaction with avian influenza virus PB1-F2 protein. They also offered help and guidance carrying out the interaction technique to understand if PB1-F2 directly associated with chicken innate components. They also provided reporter plasmids and guidance on innate reporter assays in chicken cells.
Impact	Successful publication of work. doi: 10.1099/jgv.0.001220. The cellular localization of avian influenza virus PB1-F2 protein alters the magnitude of IFN2 promoter and NF?B-dependent promoter antagonism in chicken cells.
Start Year	2016

Description	Suresh Kuchipudi and Janet Daly
Organisation	Penn State University
Country	United States
Sector	Academic/University
PI Contribution	We have hosted a co-supervised PhD student twice at The Pirbright Institute. During these visits we have helped with viral infection of primary cells for analysis of PI3K pathway perturbations and generated new GM influenza viruses to help to understand if the difference in PI3K pathway activity observed between two different avian influenza viruses in avian cells was a result of difference in the NS1 protein. In addition we have provided support to the student regarding the future directions of the project and writing up and presentation of the material. Therefore we have provided reagents, practical expertise and intellectual input.
Collaborator Contribution	The collaborators have provided access to data generated in their laboratories and intellectual input to the project.
Impact	Publication: doi: 10.1038/srep17999
Start Year	2016

Description	Suresh Kuchipudi and Janet Daly
Organisation	University of Nottingham
Department	School of Veterinary Medicine and Science Nottingham
Country	United Kingdom
Sector	Academic/University
PI Contribution	We have hosted a co-supervised PhD student twice at The Pirbright Institute. During these visits we have helped with viral infection of primary cells for analysis of PI3K pathway perturbations and generated new GM influenza viruses to help to understand if the difference in PI3K pathway activity observed between two different avian influenza viruses in avian cells was a result of difference in the NS1 protein. In addition we have provided support to the student regarding the future directions of the project and writing up and presentation of the material. Therefore we have provided reagents, practical expertise and intellectual input.
Collaborator Contribution	The collaborators have provided access to data generated in their laboratories and intellectual input to the project.
Impact	Publication: doi: 10.1038/srep17999
Start Year	2016

Description	Swine haplotyping
Organisation	Gift Of Life
PI Contribution	Prepared and shipped samples
Collaborator Contribution	Sample analysis
Impact	Data generation. Not multi-disciplinary
Start Year	2016

Description	The GCRF One Health Poultry Hub
Organisation	Royal Veterinary College (RVC)
Country	United Kingdom
Sector	Academic/University
PI Contribution	The focus of the research is to achieve sustainable global intensification of poultry meat and egg production whilst reducing risks to human and animal health and welfare. We are undertaking research to investigate the diversity of avian influenza viruses prevalent in poultry and wild birds in south east Asia.
Collaborator Contribution	Royal Veterinary College, London is leading this GCRF One Health Poultry Hub. The project will address the need to meet rising demand for poultry meat and eggs in developing countries, while minimising risk to international public health. Population growth is driving global demand for poultry, meat and egg production; this unfortunately creates conditions in which animal diseases can spread to humans ('zoonoses'). These include bacterial food poisoning and strains with avian influenza with epidemic or pandemic potential. The GCRF One Health Poultry Hub will adopt a 'One Health' approach to the issue of combatting animal-to-human diseases by bringing together a team of laboratory, clinical, veterinary and social scientists. This team will test and evaluate novel interventions. The need for safe poultry production is most urgent in South and South East Asia, so the RVC and its partners will then use their local networks in these regions to put its positive research to immediate use.
Impact	Development of international interdisciplinary partnership of 55 investigators from 13 countries to address the emerging global challenges of food security and public health associated with intensification of poultry production.
Start Year	2018

Description	The Roslin Institute
Organisation	University of Edinburgh
Department	The Roslin Institute
Country	United Kingdom
Sector	Academic/University
PI Contribution	We have been working with several groups using our molecular and genetic data to help understand cattle cellular immunology. We have also hosted a visiting scientist to provide training in molecular techniques and data analysis, This has continued and now includes members of the Centre for Tropical Livestock Health and Genetics where we are engaged in sample sharing and maximising the value of genetic data by linking different sources as much as possible.
Collaborator Contribution	Our partners have provided data and reagents as well as intellectual input to help us translate genetic diversity into functional diversity
Impact	This collaboration is multi-disciplinary, although largely immunology and associated genetics is also involves bioinformatics
Start Year	2011

Description	The effect of the microbiota on immunity to swine infleunza
Organisation	University of Surrey
Country	United Kingdom
Sector	Academic/University
PI Contribution	We wish to establish the gut and nasal microbiome in normal healthy pigs and how this is affected by influenza infection. We have collected gut samples from pigs at different stages of infection, as well as nasal swabs which we will provide to our collaborators in Surrey.
Collaborator Contribution	Our collaborators in Surrey will perform the sequencing and bioinformatics analysis to determine the microbial communities present in the samples.
Impact	No outcomes yet - we are in the process of analysing the samples.
Start Year	2017

Description	Thomas Bowden - STRUBI - University of Oxford - Glycoprotein structure collaboration
Organisation	University of Oxford
Country	United Kingdom
Sector	Academic/University
PI Contribution	Provision of data and advice about the biological properties of viral glycoproteins, e.g. their fusion capacity with various receptors, their role in determining host-range
Collaborator Contribution	Advice and technical support at the structural level supporting future project development and design
Impact	No outcomes as yet
Start Year	2018

Description	Trevor Sweeney - University of Cambridge PhD Studentship
Organisation	University of Cambridge
Country	United Kingdom
Sector	Academic/University
PI Contribution	Development of PhD studentship project in collaboration with Dr. Sweeney's lab at Cambridge and application at Pirbright.
Collaborator Contribution	Development of PhD studentship project and application at Cambridge.
Impact	The PhD studentship has been funded and is currently been advertised.
Start Year	2018

Description	University of Glasgow collaborations (Sam Wilson and Massimo Palmarini)
Organisation	University of Glasgow
Department	MRC - University of Glasgow Centre for Virus Research
Country	United Kingdom
Sector	Academic/University
PI Contribution	Scientific discussion and reagent sharing contributing to grant applications and active research projects
Collaborator Contribution	Scientific discussion and reagent sharing contributing to grant applications and active research projects
Impact	Successful MRC grant application
Start Year	2015

Description	University of Liverpool Proteomics Collaboration
Organisation	University of Liverpool
Country	United Kingdom
Sector	Academic/University
PI Contribution	Generation of samples to analyse the membranous proteome of cells infected with paramyxoviruses.
Collaborator Contribution	Proteomic analysis of samples and technical support. Access to previous data-sets and expertise.
Impact	Preliminary data sets only to date.
Start Year	2018

Title	Chicken IFITMs
Description	The present invention provides an avian cell in which the expression or activity of one or more of the following genes, or a homologue thereof: Chicken IFITM1 (SEQ ID No. 1); Chicken IFITM2 (SEQ ID No. 2) and Chicken IFITM3 (SEQ ID No. 3) is reduced. The invention also provides methods for passaging viruses in avian cells, embryos and/or avian cell lines which have reduced expression of one or more IFITM genes and methods which involve investigating the sequence of one or more of the following genes, or a homologue thereof: Chicken IFITM1 (SEQ ID No. 1); Chicken IFITM2 (SEQ ID No. 2) and Chicken IFITM3 (SEQ ID No. 3).
IP Reference	WO2014195692
Protection	Patent granted
Year Protection Granted	2018
Licensed	Commercial In Confidence
Impact	The present invention provides an avian cell in which the expression or activity of one or more of the following genes, or a homologue thereof: Chicken IFITM1 (SEQ ID No. 1); Chicken IFITM2 (SEQ ID No. 2) and Chicken IFITM3 (SEQ ID No. 3) is reduced. The invention also provides methods for passaging viruses in avian cells, embryos and/or avian cell lines which have reduced expression of one or more IFITM genes and methods which involve investigating the sequence of one or more of the following genes, or a homologue thereof: Chicken IFITM1 (SEQ ID No. 1); Chicken IFITM2 (SEQ ID No. 2) and Chicken IFITM3 (SEQ ID No. 3). This patent has enabled us to leverage funding for continued work.

Description	Talk Presented at 2nd International Congress of Veterinary Microbiology, 16th -19th October 2018. Sherwood Breezes Resort-Lara, Antalya, Turkey.
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Professional Practitioners
Results and Impact	Conference discussed improving prevention and control of viral diseases of poultry.
Year(s) Of Engagement Activity	2018

Description	"Challenges for Poultry Industry". Organised by Fakieh Poultry at the Ritz-Carlton Hotel in Jeddah, Saudi Arabia, 2nd -3rd December 2018,
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Professional Practitioners
Results and Impact	Conference discussed prevention and control of infectious diseases affecting poultry production.
Year(s) Of Engagement Activity	2018

Description	"Interactive session with poultry stakeholders". Title: Overview of Avian Influenza group Research at The Pirbright Institute. Presented at University of Veterinary and Animal Sciences (UVAS) Lahore, Pakistan, 3rd August 2018.
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Professional Practitioners
Results and Impact	The workshop discussed the economic impacts and prevention strategies against avian influenza viruses affecting poultry production and zoonotic infections.
Year(s) Of Engagement Activity	2018

Description	2. Overview of Avian Influenza group Research at The Pirbright Institute. Presented at "Interactive session with poultry stakeholders" at University of Veterinary and Animal Sciences (UVAS) Lahore, Pakistan, 3rd August 2018.
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Professional Practitioners
Results and Impact	Dissemination of research Project outcomes These include presenting research data for vaccine seed strains with emergent field avian influenza variants; The zoonotic risk posed by emerging avian influenza viruses circulating in the wild birds and poultry. Impact of virus evolution on vaccine efficacy and persistence in poultry.
Year(s) Of Engagement Activity	2018

Description	360 degree Lab Animal Tour (and film) of The Plowright Building, The Philip Mellor Insectary, and Animal Isolation Facilities
Form Of Engagement Activity	Engagement focused website, blog or social media channel
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Public/other audiences
Results and Impact	The website provides unique access to the animal research facilities at The Pirbright Institute which support its world-leading scientific research into livestock viral diseases and helps save countless animal and potentially human lives, globally. Produced and filmed by Understanding Animal Research, the Lab Animal Tour was filmed at Pirbright, and at three other research centres, and provides the viewer with an interactive 360 virtual tour of the animal facilities.
Year(s) Of Engagement Activity	2017
URL	http://www.labanimaltour.org/pirbright

Description	A talk at a symposium entitled: Using the 3Rs to support good science
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	National
Primary Audience	Professional Practitioners
Results and Impact	I gave a talk at a Symposium held at The Pirbright Institute entitled: "Using the 3Rs to support good science". The talk was entitled: "A chicken primary B cell culture model to study the pathogenesis and improve the control of immunosuppressive viruses of poultry"
Year(s) Of Engagement Activity	2017

Description	A talk at the British Poultry Diseases Group meeting
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	National
Primary Audience	Industry/Business
Results and Impact	The Poultry Diseases Group meet quarterly and is comprised of representatives from private veterinary practices, vaccine and pharmaceutical industries, and the Animal and Plant Health Agency (APHA). I gave a talk at one of the meetings outlining my research to date and future directions.
Year(s) Of Engagement Activity	2017

Description	Amy Lewis, The Scientist, 15/08/2017 re gene drive and Predator Free NZ 2050
Form Of Engagement Activity	A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Media (as a channel to the public)
Results and Impact	Provided information and quotes for article on potential use of gene drives as part of New Zealand effort to eradicate invasive predators
Year(s) Of Engagement Activity	2017
URL	https://www.the-scientist.com/?articles.view/articleNo/50180/title/Driving-Down-Pests/

Description	Ash Manor GCSE Mentoring 2018 - BA
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	Local
Primary Audience	Schools
Results and Impact	Stimulating increased interest in research
Year(s) Of Engagement Activity	2018

Description	BBC Today programme 24/01/2019
Form Of Engagement Activity	A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme?	No
Geographic Reach	National
Primary Audience	Media (as a channel to the public)
Results and Impact	Interview on BBC Today programme regarding potential development and use of gene drives in mice
Year(s) Of Engagement Activity	2019
URL	https://www.bbc.co.uk/sounds/play/m00025bt

Description	BBSRC website article: Halloween
Form Of Engagement Activity	Engagement focused website, blog or social media channel
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Public/other audiences
Results and Impact	Article on BBSRC website: Halloween Creepy Crawlies
Year(s) Of Engagement Activity	2017
URL	http://www.bbsrc.ac.uk/news/fundamental-bioscience/2017/171031-f-uk-creepiest-creepy-crawlies/

Description	Big Bang Fair South East - RC
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	Regional
Primary Audience	Public/other audiences
Results and Impact	Regional science festival/event - changes in public attitudes and stimulating increased interest in research
Year(s) Of Engagement Activity	2018

Description	Biotech YES, Syngenta Jealott's Hill, 05 Oct 2017
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	National
Primary Audience	Postgraduate students
Results and Impact	Teams of postgraduate students from a number of universities and institutes attended talk as part of Biotech YES (Young Entrepreneurs' Scheme). Follow-up discussion influenced several aspects of their approach to entrepreneurship both within the competition and more generally
Year(s) Of Engagement Activity	2014,2015,2016,2017

Description	Bluetongue and Wildlife (15 minute presentation on Epizootic Haemorhagic Disease Virus), (July/Pirbright, UK)
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	National
Primary Audience	Policymakers/politicians
Results and Impact	Policymakers reported greater interest in deer-associated diseases including EHDV and BTV
Year(s) Of Engagement Activity	2018

Description	CABI_Research activity of Avian Immunology Group at The Pirbright Institute
Form Of Engagement Activity	Engagement focused website, blog or social media channel
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Media (as a channel to the public)
Results and Impact	Release of research activity within Avian Immunology group at The Pirbright Institute
Year(s) Of Engagement Activity	2018

Description	CBD Open-ended online forum on synbio 06-10/2017
Form Of Engagement Activity	A formal working group, expert panel or dialogue
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Policymakers/politicians
Results and Impact	UN Convention on Biodiversity. Policy- discussion on synthetic biology: nominated participant in the Open Ended Online Forum on Synthetic biology as established in decision XII/24 of the Conference of the Parties to the Convention on Biological Diversity (COP) and extended in COP decision XIII/17 which ran from June to Oct 2017 (approx.)
Year(s) Of Engagement Activity	2017
URL	http://bch.cbd.int/synbio/open-ended/discussion/

Description	Cafe Scientifique, Bradfield School, West Berkshire: "How insects spread viruses"
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	Local
Primary Audience	Public/other audiences
Results and Impact	Cafe Scientifique is a public forum for exploring the latest ideas in science and technology. Meetings take place in cafes, bars, restaurants and even theatres, but always outside a traditional academic context. I presented to an audience of the general public and answered questions.
Year(s) Of Engagement Activity	2017

Description	Cafe Scientifique, Reading: "How insects spread viruses"
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	Local
Primary Audience	Public/other audiences
Results and Impact	Cafe Scientifique is a public forum for exploring the latest ideas in science and technology. Meetings take place in cafes, bars, restaurants and even theatres, but always outside a traditional academic context. I presented to an audience of the general public and answered questions.
Year(s) Of Engagement Activity	2007,2017

Description	Camberley Library Fun Palace 2018 - JM
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	Local
Primary Audience	Public/other audiences
Results and Impact	Changes in public attitudes and stimulating increased interest in research
Year(s) Of Engagement Activity	2018

Description	Caroline Seydel Genetic Literacy Project re GM mosquitoes/gene drive/Zika
Form Of Engagement Activity	A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Media (as a channel to the public)
Results and Impact	Provided background material and quotes for article on GM mosquitoes and control of Zika virus.
Year(s) Of Engagement Activity	2017
URL	https://geneticliteracyproject.org/2018/01/29/waging-war-mosquito-split-gene-drive-hinder-spread-zik...

Description	Centre of excellence for research on avian diseases (CERAD)
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Other audiences
Results and Impact	I was invited to present my work at the UK-China Centre of excellence for research on avian diseases (CERAD) meeting attended by researchers from UK, China and Thailand. There was lots of discussion about the research and future directions, including potential collaborations.
Year(s) Of Engagement Activity	2017

Description	Cheltenham Science Festival
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	National
Primary Audience	Public/other audiences
Results and Impact	Institute stand at the festival covering the Institute science in general, and transmission and gene editing to control disease, in particular.
Year(s) Of Engagement Activity	2018

Description	Cheltenham Science Festival 2018 - JA
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	National
Primary Audience	Public/other audiences
Results and Impact	Major science festival - changes in public attitudes and stimulating increased interest in research
Year(s) Of Engagement Activity	2018

Description	Cheltenham Science Festival 2018 - JM
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	National
Primary Audience	Public/other audiences
Results and Impact	Major science festival - changes in public attitudes and stimulating increased interest in research
Year(s) Of Engagement Activity	2018

Description	Cheltenham Science Festival 2018 - JP
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	National
Primary Audience	Public/other audiences
Results and Impact	Major national science festival - changes in public attitudes and stimulating increased interest in research
Year(s) Of Engagement Activity	2018

Description	Cheltenham Science Festival 2018 - RC
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	National
Primary Audience	Public/other audiences
Results and Impact	Major science festival - changes in public attitudes and stimulating increased interest in research
Year(s) Of Engagement Activity	2018

Description	Cheltenham Science Festival 2018 - SV
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	National
Primary Audience	Public/other audiences
Results and Impact	Display at major science festival: changes in public attitudes and stimulating increased interest in research
Year(s) Of Engagement Activity	2018

Description	Cheltenham Science Festival 2018-DP
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	National
Primary Audience	Public/other audiences
Results and Impact	Major national science festival - changes in public attitudes and stimulating increased interest in research
Year(s) Of Engagement Activity	2018

Description	Combating avian influenza through systematic analysis of antigenic drift, genetic variation, and development of novel diagnostic tools and vaccines. Presented at as PI of the ZELS project at ZELS grant holders and Stakeholders meeting in Hanoi, Vietnam 23th-24th January 2017.
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Professional Practitioners
Results and Impact	Engagement and dissemination of research outcome to representatives of research funding bodies such as Department for International Development (DFID), Medical Research Council (MRC) and the researchers working on different research projects funded by BBSRC under Zoonoses and Emerging Livestock Systems (ZELS).
Year(s) Of Engagement Activity	2018

Description	Conference session organiser at MMC2017 - Host Pathogen Interactions
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Professional Practitioners
Results and Impact	Chair for the Imaging Host-Pathogen Interactions session run at MMC2017 (and in previous years). Session usually attracts approx. 30 delegates from national/international backgrounds, mostly academic. Talks sparked discussions and networking opportunities.
Year(s) Of Engagement Activity	2012,2014,2015,2017

Description	Coronavirus lecture MSc students (Surrey)
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	Regional
Primary Audience	Postgraduate students
Results and Impact	A lecture was presented on the replication, cellular interactions and pathogenesis of coronaviruses to 15-20 MSc students from University of Surrey. The students were engaged and interested, answering and asking questions during the session.
Year(s) Of Engagement Activity	2016,2018

Description	DARPA visit April 2017
Form Of Engagement Activity	Participation in an open day or visit at my research institution
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Supporters
Results and Impact	Hosted visit by US funding agency programme manager to The Pirbright Institute
Year(s) Of Engagement Activity	2017

Description	Diamond open day
Form Of Engagement Activity	Participation in an open day or visit at my research institution
Part Of Official Scheme?	No
Geographic Reach	National
Primary Audience	Public/other audiences
Results and Impact	Over 300 people visited our stand at the open day, in which we described the importance of our work for food security and public health. Children from all ages were involved and learned about viruses and played with the huge virus particle that we had produced. One of the major impact was that we described to several people why we work on some viruses that we do not have them in the UK, and the importance of the work for the UK and UK economy and public health was explained.
Year(s) Of Engagement Activity	2017
URL	http://www.diamond.ac.uk/Public/VisitUs.html

Description	Discuss gene drives with NZ Parliamentary Commissioner
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Policymakers/politicians
Results and Impact	Met NZ Parliamentary Commissioner to provide background information on, and discuss, gene drive systems. New Zealand is considering genetic and other control methods for invasive pests.
Year(s) Of Engagement Activity	2018

Description	EM-UK: A national network for electron microscopists
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Professional Practitioners
Results and Impact	Founder and Chair of EM-UK, a national network (with international participants) for electron microscopists. Includes a one or two day meeting with invited speakers, and email discussion list for questions, comments, advertising courses, advertising jobs. It has proved very popular with over 200 people registering for the discussion list, and 90+ delegates at each meeting. Administration is handled by the Royal Microscopical Society.
Year(s) Of Engagement Activity	2016,2017,2018

Description	Encouraging women into science and engineering STEM.
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	Local
Primary Audience	Schools
Results and Impact	MH Visited Warwick School, Redhill to talk to secondary students about careers in STEM. A very positive outcome and well received.
Year(s) Of Engagement Activity	2018

Description	European Society Vector Ecology (Invited Chair for two sessions and speaker for 15min talk); (October/Palermo, Sicily)
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Professional Practitioners
Results and Impact	Chaired two sessions, one on vector-borne diseases, one on scientific networks. Latter included a discussion with early career researchers. Main outcome was coordination between active networks including The Gnatwork.
Year(s) Of Engagement Activity	2018
URL	http://www.sove.org/European%20SOVE%20folder/greecescientificprogram/palermo_Scientific_program_revi...

Description	Evolution of H9N2 avian influenza virus under immune pressure. Presented at 10th International Symposium on Avian Influenza, 15th - 18th April 2018, The Grand Hotel, Brighton, UK.
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Professional Practitioners
Results and Impact	The primary aim of the workshop was to forge long-term research partnerships between early-career researchers, livestock industry and national disease control authorities in the UK and the Philippines. In addition, the activity hopes provide information about the emerging and next generation diagnostic and vaccine strategies and their utilization to reduce the impact of viral diseases on livestock and poultry.
Year(s) Of Engagement Activity	2019
URL	https://www.slideshare.net/zerep_cire/2019-newton-agham-researcher-links-workshop-vaccines-and-diagn...

Description	Ewen Callaway Nature 08/01/2018
Form Of Engagement Activity	A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Media (as a channel to the public)
Results and Impact	Provided expert background information for article in Nature regarding engineering species barriers, described by journalist as "very helpful" in followup email.
Year(s) Of Engagement Activity	2018

Description	FAO/IAEA Coordinated Research Programme on methods of sex separation in mosquitoes; Research coordination meeting Bangkok 19-23 February 2018
Form Of Engagement Activity	A formal working group, expert panel or dialogue
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Professional Practitioners
Results and Impact	FAO/IAEA Coordinated Research Programme on exploring genetic, molecular, mechanical, and behavioral methods of sex separation in mosquitoes; aimed at promoting specific areas of research, exchange of ideas and networking among the experts in the field.
Year(s) Of Engagement Activity	2013,2015,2016,2018

Description	FLI - presentation
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	Local
Primary Audience	Postgraduate students
Results and Impact	Presentation of current research to the FLI - Germany to build collaborations 19/20 November 2018
Year(s) Of Engagement Activity	2018

Description	Facebook Live: Infectious Disease Portrayals in Pop Culture
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Public/other audiences
Results and Impact	Infectious diseases are a frequent topic in movies. The CDC has used zombies to talk about infectious disease and disaster preparedness, and infotainment has flamed fears of Ebola. I took part in a Facebook Live chat organised by the AAAS Public Engagement team about why infectious diseases are so often the topic of pop culture discussions; what movies, books, and other pop culture gets right and wrong about infectious disease; and how scientists can use pop culture as a jumping-off point for public engagement. The chat was broadcast live on the AAAS Facebook page.
Year(s) Of Engagement Activity	2017
URL	https://www.aaas.org/event/facebook-live-infectious-disease-portrayals-pop-culture

Description	Farnborough College Apprenticeship Fair- 20th March 2018
Form Of Engagement Activity	Participation in an open day or visit at my research institution
Part Of Official Scheme?	No
Geographic Reach	Local
Primary Audience	Schools
Results and Impact	Careers fair attended by approx. 100 school children. Pirbright demonstrated the multiple ways that research organisations can provide jobs including scientists, engineers and computer operators.
Year(s) Of Engagement Activity	2018

Description	Farnborough futures careers event
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	Regional
Primary Audience	Schools
Results and Impact	The careers event aimed to introduce the range of careers available at Pirbright. In addition I discussed my own career progression and highlighted the range of possible careers that I could have entered. Finally, I discussed some details of the research with interested students. The students had a wide range of backgrounds and career aims but were interested to hear about the Institute and the sort of work opportunities available. Several of them planned to follow up by investigating future job opportunities at Pirbright.
Year(s) Of Engagement Activity	2019

Description	Food security and the control of infectious disease in livestock
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Professional Practitioners
Results and Impact	A workshop was organized by myself at St Catherine's College Oxford for the duration of three days. Representative from industry, funding agencies and media (publishers) attended this workshop. Over 20 delegates from Egypt and 20 delegates from the UK attended this meeting. The purpose of this workshop/ meeting was to promote international collaborations, provide training for undergraduate students, promote the importance of managing and controlling infectious disease in livestock and public health.
Year(s) Of Engagement Activity	2016

Description	Gene Drive Resarch Sponsors and Supporter's Forum meeting Montreal Sept 2018
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Supporters
Results and Impact	Forum met to discuss the state of the art and future considerations for gene drive research. Ongoing deliberations including specific follow-up meetings.
Year(s) Of Engagement Activity	2018

Description	Gnatwork Website
Form Of Engagement Activity	Engagement focused website, blog or social media channel
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Professional Practitioners
Results and Impact	New website established to act as focalpoint of project.
Year(s) Of Engagement Activity	2018
URL	http://www.gnatwork.ac.uk/

Description	Guest lecturer MSc Imaging course at University of Copenhagen
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Postgraduate students
Results and Impact	25 internal students from University of Copenhagen visited the core imaging facility for three days during which time I delivered lectures and interacted with the students during practical workshops. This sparked questions and discussion relating to their individual projects.
Year(s) Of Engagement Activity	2018

Description	H9N2 avian influenza viruses: impact of evolutionary changes on virus antigenicity, receptor binding and zoonotic potential. Presented at "Conference on Animal Infectious Diseases and Human Health" held at The Veterinary Biotechnology Branch of Chinese Association of Animal Science and Veterinary Medicine & the Veterinary Immunology Branch of Chinese Society for Immunology. August 7th-10th, 2018 Harbin, China.
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Postgraduate students
Results and Impact	Focus of the conference was to improve control against infectious diseases affecting poultry and livestock.
Year(s) Of Engagement Activity	2018

Description	H9N2 avian influenza viruses: impact of evolutionary changes on virus antigenicity, receptor binding and zoonotic potential. Presented at "Conference on Animal Infectious Diseases and Human Health" held at The Veterinary Biotechnology Branch of Chinese Association of Animal Science and Veterinary Medicine & the Veterinary Immunology Branch of Chinese Society for Immunology. August 7th-10th, 2018 Harbin, China.
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Professional Practitioners
Results and Impact	Dissemination of research outcomes to prevent and control of infectious diseases of animals and humans. - Avian influenza virus evolution and efficacy. -
Year(s) Of Engagement Activity	2018

Description	Holly Kernot, Veterinary Times, 13/06/2017
Form Of Engagement Activity	A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme?	No
Geographic Reach	National
Primary Audience	Media (as a channel to the public)
Results and Impact	Discussed potential of manipulating mosquito sex determination genes as tools for novel mosquito genetic control approaches.
Year(s) Of Engagement Activity	2017
URL	https://www.vettimes.co.uk/news/study-to-be-transformational-in-mosquito-control-strategies/

Description	Holt School Surrey. Bee meadow & A-level outreach Talk.
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	Local
Primary Audience	Schools
Results and Impact	I discussed the importance of pollinators for biodiversity and food security with the younger students. I also discussed careers in science with A-Level students. There was good interaction and many questions surrounding both topics.
Year(s) Of Engagement Activity	2018

Description	Human Infection Challenge Models Network
Form Of Engagement Activity	A formal working group, expert panel or dialogue
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Policymakers/politicians
Results and Impact	HIC-Vac is an international network of researchers who are developing human infection challenge (HIC) studies to accelerate the development of vaccines against pathogens of high global impact. The goal is to foster an engaged and interactive community of international researchers to promote open sharing of knowledge and expertise, generate new ideas, support and share best practice, and form new cross-discipline collaborations.
Year(s) Of Engagement Activity	2018

Description	IUCN Technical Subgroup on Synthetic Biology and Biodiversity Conservation (meeting 12-15 April and report)
Form Of Engagement Activity	A formal working group, expert panel or dialogue
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Policymakers/politicians
Results and Impact	Participated as member in Technical Subgroup on Synthetic Biology and Biodiversity Conservation of International Union for Conservation of Nature (IUCN). Meetings, online discussion and ultimately a report to Task Force and will ultimately go to IUCN General Assembly.
Year(s) Of Engagement Activity	2018,2019

Description	Immuno-pathobiology of H9N2 avian influenza viruses: looking at how the viruses evolve and persist in poultry. Presented at "Techniques for Healthy Farming and Diseases Prevention & Control of Livestock and Poultry. Beijing, August 13th-15th, 2018.
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Postgraduate students
Results and Impact	Dissemination of research outputs. Improvement of vaccines and diagnostics.
Year(s) Of Engagement Activity	2018

Description	InFocus article (Jennifer Simpson)
Form Of Engagement Activity	A magazine, newsletter or online publication
Part Of Official Scheme?	No
Geographic Reach	National
Primary Audience	Professional Practitioners
Results and Impact	InFocus review article for EM-UK meeting (2017)
Year(s) Of Engagement Activity	2017

Description	Influenza update 2018
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	National
Primary Audience	Postgraduate students
Results and Impact	Approx. 70 scientists attended a Influenza update meeting at which our group presented an oral presentation and poster presentation on our work. These actions resulted in establishment of new collaborations for ongoing work and the initiation of a joint grant writing exercise.
Year(s) Of Engagement Activity	2018

Description	Influenza update meeting (Pengxiang Chang)
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	National
Primary Audience	Professional Practitioners
Results and Impact	presented work entitled: Antigenic characterization of avian influenza H7N9 virus by in vitro immune escape mutant selection method.
Year(s) Of Engagement Activity	2018

Description	InnovSur : Conference in IRD Montpellier on Insect Vector Surveillance
Form Of Engagement Activity	A formal working group, expert panel or dialogue
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Professional Practitioners
Results and Impact	Working group on standardisation of techniques for surveillance of insect vectors. Included break out group brainstorming and subsequent commentaries. Total audience approximately 110 people for main lectures.
Year(s) Of Engagement Activity	2018
URL	https://www.alphavisa.com/isessah-innovsur/2018/

Description	Innovate Guildford
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	Regional
Primary Audience	Public/other audiences
Results and Impact	Institute stand at Innovate Guildford science festival. Visitors to stand able to discuss issues related to animal diseases and their control (with a particular focus on influenza and on use of genetic modification technologies)
Year(s) Of Engagement Activity	2017

Description	Innovate Guildford 17 March 2018
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	Local
Primary Audience	Public/other audiences
Results and Impact	Talk and exhibit at Innovate Guildford - >100 for talk, probably >500 for exhibit. Both sparked questions and discussion afterwards.
Year(s) Of Engagement Activity	2018

Description	Innovate Guildford 2018
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	Regional
Primary Audience	Public/other audiences
Results and Impact	Institute stand at the Innovate Guildford event, covering the work of the Institute in general and genetic modification through gene editing specifically.
Year(s) Of Engagement Activity	2018
URL	https://www.guildford.gov.uk/IG18

Description	Innovate Guildford 2018 - JM
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	Regional
Primary Audience	Public/other audiences
Results and Impact	Regional science/innovation event - changes in public attitudes and stimulating increased interest in research
Year(s) Of Engagement Activity	2018

Description	Innovate Guildford 2018 - SB
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	Regional
Primary Audience	Public/other audiences
Results and Impact	Regional science festival - changes in public attitudes and stimulating increased interest in research
Year(s) Of Engagement Activity	2018

Description	Innovate Guildford 2018 - SV
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	Regional
Primary Audience	Public/other audiences
Results and Impact	Display at regional science/innovation festival - changes in public attitudes and stimulating increased interest in research
Year(s) Of Engagement Activity	2018

Description	Innovate Guildford, ND
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	Regional
Primary Audience	Public/other audiences
Results and Impact	Presented at the Pirbright Institute stand discussing science performed by the Institute with the general public. The stand focussed on an exhibit on avian influenza virus but more general virology topics were also discussed including our research.
Year(s) Of Engagement Activity	2017

Description	Innovate Guilford
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	Local
Primary Audience	Public/other audiences
Results and Impact	Dr Broadbent gave a talk at 'Innovate Guilford' Festival outlining the work we are doing with Avian Virus Vaccines, including those against IBDV. The aim was to raise public awareness on different avian viruses, and genetically modified vaccines.
Year(s) Of Engagement Activity	2017

Description	Insect Festival 2017
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	Regional
Primary Audience	Public/other audiences
Results and Impact	Ran a stand on blood-sucking arthropods and their role in the transmission of disease at the Royal Entomological Society's Insect Festival. Talked to public, answered questions. Lots of enquiries about ticks, midges, mosquitoes and large biting flies (particularly horseflies).
Year(s) Of Engagement Activity	2017
URL	https://www.yorkmuseumstrust.org.uk/whats-on/events/insect-festival-2017/

Description	International Avian Respiratory Disease Conference
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Industry/Business
Results and Impact	Michael presented his research at the 2018 International Avian Respiratory Disease Conference in the US to an audience of avian researchers and representatives form the poultry veterinary vaccine industry. This sparked discussion and potential future collaborations
Year(s) Of Engagement Activity	2018

Description	International Day of Women and Girls in Science
Form Of Engagement Activity	Engagement focused website, blog or social media channel
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Public/other audiences
Results and Impact	I gave a quote as a STEM ambassador about why I think it is important to make sure women and girls have the same opportunities in STEM. This was shared on social media to celebrate International Day of Women and Girls in Science, a UN-led initiative.
Year(s) Of Engagement Activity	2019

Description	International Women's Day video for social media
Form Of Engagement Activity	Engagement focused website, blog or social media channel
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Public/other audiences
Results and Impact	Three team members were interviewed about their careers in science for International Women's Day. The videos were posted on Pirbright's website, Facebook and Twitter to celebrate what we love about working in science.
Year(s) Of Engagement Activity	2018

Description	Invitation to give keynote talk on 'Control of avian diseases: Challenges and opportunities'
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Professional Practitioners
Results and Impact	Gave the Keynote talk on 'Control of avian diseases: Challenges and opportunities' at this International Symposium organized with an aim to gather international virology experts from both human and veterinary fields to share research experience, update findings and contribute their expertise through presentation and interactive discussion. The topics will cover a wide range of virology research common in both human and animal aspects. In addition, technological platforms such as vaccine development, and novel assays will be emphasized and addressed.
Year(s) Of Engagement Activity	2018
URL	http://www.biotec.or.th/virus-symposium2018/index.php

Description	Invited Chair at European Congress of Entomology (Naples,July)
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Professional Practitioners
Results and Impact	Chaired session on vector-borne diseases at meeting and subsequent question sessions with 15 minute presentation on Culicoides biting midges.
Year(s) Of Engagement Activity	2018
URL	http://www.ece2018.com/scientific-programme/day-1-monday-2-july/

Description	Invited Seminar University College London
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	National
Primary Audience	Professional Practitioners
Results and Impact	Invited seminar at UCL to describe my ongoing research
Year(s) Of Engagement Activity	2018

Description	Invited participant, Improving the Use of Evidence in Policymaking in the UK Government (Parliamentary Office of Science and Technology)
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	National
Primary Audience	Policymakers/politicians
Results and Impact	Invited to attend meeting to launch POST report "Improving the Use of Evidence in Policymaking in the UK Government" as a result of previous collaboration.
Year(s) Of Engagement Activity	2017
URL	http://www.sciencecampaign.org.uk/resource/casereportimprovingtheuseofevidence2017.html

Description	Invited presentation at the Friedrich Loeffler Institute (January; Riems)
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Professional Practitioners
Results and Impact	Formal presentation on biting midges and bluetongue to professional audience with question session and collaborative meetings
Year(s) Of Engagement Activity	2019

Description	Invited presentation: 'Beyond Jenner: The Future of Vaccines', Discovery Day, Dr Jenner House Museum and Gardens
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	National
Primary Audience	Public/other audiences
Results and Impact	Invited by the British Society for Immunology to give a presentation on vaccine research at the 'Beyond Jenner: The Future of Vaccines', Discovery Day, Dr Jenner House Museum and Gardens, Berkeley, UK.
Year(s) Of Engagement Activity	2018

Description	Invited seminar at UC Riverside 05/05/2017
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Professional Practitioners
Results and Impact	Invited seminar at University of California, Riverside
Year(s) Of Engagement Activity	2017

Description	Invited seminar at the National Health Research Institutes, Taiwan. 27 Feb 2018
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Professional Practitioners
Results and Impact	A presentation describing research in my group on mosquito developmental biology and its potential uses for mosquito control; attended by primarily by members of the National Mosquito-Borne Diseases Control Research Center.
Year(s) Of Engagement Activity	2018

Description	Invited seminar at the University of Nottingham seminar series
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	Regional
Primary Audience	Professional Practitioners
Results and Impact	An invited seminar entitled: "Arbovirus encephalitis: from tissue culture, to mosquitoes to mice" was delivered. Discussions for potential collaborations followed.
Year(s) Of Engagement Activity	2017

Description	Invited seminar in Public Health England- Porton Down
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	Local
Primary Audience	Professional Practitioners
Results and Impact	Invited seminar entitled: "Innate immune responses and pathogenesis of alphavirus infections". Numerous questions were asked and requests for collaborations were made.
Year(s) Of Engagement Activity	2017

Description	Invited speaker - Moredun
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	National
Primary Audience	Professional Practitioners
Results and Impact	Presentation on Bioimaging capabilities at Pirbright to Moredun Institute. Attended by Head of Bioimaging at Roslin too, increased links between these institutes.
Year(s) Of Engagement Activity	2016,2018

Description	Invited speaker - RMS EM School
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Professional Practitioners
Results and Impact	Invited to lecture on certain aspects of electron microscopy. I provided advice on individuals difficulties they had with sample preparation. Delegates had the opportunity to ask me any questions then had relating to any sample type.
Year(s) Of Engagement Activity	2018

Description	Invited speaker at 9th Neuroinfections Conference, Bialowieza - Poland
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Professional Practitioners
Results and Impact	An invited presentation entitled: "Using small RNAs to fight encephalitic arboviruses" was delivered. The presentation generated a lot of interest and questions.
Year(s) Of Engagement Activity	2017

Description	Invited speaker at 9th Neuroinfections Conference, Bialowieza - Poland
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Professional Practitioners
Results and Impact	An invited talk entitled: "Using small RNAs to fight encephalitic arboviruses" was delivered. Numerous questions were asked.
Year(s) Of Engagement Activity	2017

Description	Invited speaker at APHA surveillance meeting
Form Of Engagement Activity	A formal working group, expert panel or dialogue
Part Of Official Scheme?	No
Geographic Reach	National
Primary Audience	Professional Practitioners
Results and Impact	Invited speaker on insect surveillance at Animal and Plant Health Agency meeting with discussion around current studies.
Year(s) Of Engagement Activity	2018

Description	Invited speaker at Imperial College
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	Regional
Primary Audience	Professional Practitioners
Results and Impact	Gave a talk on our recent research in swine influenza. Discussions with PIs at Imperial after the talk. Established collaborations.
Year(s) Of Engagement Activity	2018

Description	Invited speaker at South Wilts Virus Group (SWVG)
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	Regional
Primary Audience	Professional Practitioners
Results and Impact	A talk entitled: "The role of small RNAs in arbovirus infections". Multiple questions were asked and potential for collaborations was identified.
Year(s) Of Engagement Activity	2017

Description	Invited speaker at a workshop on arboviruses and their vectors in University of Malaya - Malaysia
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Professional Practitioners
Results and Impact	A talk entitled: " The role of small RNAs in arbovirus infections" was delivered. Multiple questions and requests for collaborations followed the presentation.
Year(s) Of Engagement Activity	2017

Description	Invited speaker at the British VeterinaryPoultry Association 2018 meeting at Boehringer Ingelheim
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	National
Primary Audience	Industry/Business
Results and Impact	I was invited to present my research findings at the British Veterinary Poultry Association 2018 meeting, held at Boehringer Ingelheim.
Year(s) Of Engagement Activity	2018

Description	Invited speaker, Merseyside BSI Affinity group
Form Of Engagement Activity	A formal working group, expert panel or dialogue
Part Of Official Scheme?	No
Geographic Reach	Regional
Primary Audience	Professional Practitioners
Results and Impact	I described the methods we used to identifyTissue resident memory cells in pigs, which sparked a lively discussion and interest in the technique. As a result I have established two collaborations with colleagues from Liverpool.
Year(s) Of Engagement Activity	2018

Description	Invited speaker, Surrey University
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	Local
Primary Audience	Postgraduate students
Results and Impact	Under- and postgraduate students attended a talk I gave at the Department of Biological Sciences in Surrey. There was a lively discussion afterwards and as a result I have established a new collaboration.
Year(s) Of Engagement Activity	2018

Description	Invited speaker, Vienna Veterinary School
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Postgraduate students
Results and Impact	Gave a guest lecture at the Veterinary school in Vienna. There were a lot of questions and discussion after the talk. As a result established collaboration with Gerner Wilhelm's group.
Year(s) Of Engagement Activity	2019
URL	https://www.vetmeduni.ac.at/de/graduate-school-pig-and-poultry-medicine/

Description	Invited to talk at the International Conference on Building Human and Animal Health Capacities organised by the Jordan University of Science and Technology
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Professional Practitioners
Results and Impact	Participated in the discussion and talked on 'The recent advances in vaccination-based control of poultry diseases'. The need for increased awareness of the pathogen diversity in the design of vaccines and the opportunities from advances in vaccine technologies were discussed
Year(s) Of Engagement Activity	2017
URL	http://www.just.edu.jo/conferences/bhahc/Pages/default.aspx

Description	JN - Woking school 2018
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	Local
Primary Audience	Schools
Results and Impact	Careers Fair at a local school encouraging children to consider science as a career and representing the institute in the local community.
Year(s) Of Engagement Activity	2008

Description	Jennifer Simpson - Big Bang Competition judge
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	National
Primary Audience	Schools
Results and Impact	The Big Bang Competition recognises and rewards young people's achievements in all areas of science, technology, engineering and maths (STEM), whilst providing them with the opportunity to build their skills and confidence in project-based work. The Competition is open to all UK residents in full-time education or training.
Year(s) Of Engagement Activity	2017,2018

Description	Jennifer Simpson - Careers Fair at Woking High School
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	Local
Primary Audience	Schools
Results and Impact	Pirbright Institute stand at the careers fair. Speak to students interested in a career in science. Sparked questions and discussions.
Year(s) Of Engagement Activity	2018

Description	Jennifer Simpson - Girls into STEM
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	Local
Primary Audience	Schools
Results and Impact	Outreach activity for girls in local schools to demonstrated the variety of careers available within the STEM sector.
Year(s) Of Engagement Activity	2018

Description	Jennifer Simpson - InFocus article on Bioimaging in high containment
Form Of Engagement Activity	A magazine, newsletter or online publication
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Professional Practitioners
Results and Impact	Magazine article describing the facilities for imaging in high containment at Pirbright.
Year(s) Of Engagement Activity	2018

Description	Keynote speaker - Danish Virology Society Annual Meeting
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Professional Practitioners
Results and Impact	I was personally invited to be keynote speaker at the annual meeting of the Danish Virology Society, to talk about high containment microscopy of cell biology of host-virus interactions. The meeting was held at the State Serum Institute in Copenhagen, followed by a social evening which enabled me to add new contacts into my network. This invitation came direct from the Danish Virology Society, independent of the work I already do with the University of Copenhagen.
Year(s) Of Engagement Activity	2018

Description	Keynote talk at the World Veterinary Poultry Association meeting
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Professional Practitioners
Results and Impact	Invited to give the keynote talk on 'Avian tumour viruses: their contribution to our understanding of cancer' at the World Veterinary Poultry Association XXth Congress in Edinburgh
Year(s) Of Engagement Activity	2017
URL	http://www.wvpac2017.com/

Description	Kingdown School Warminster Wiltshire talk to 6th form students about career in science.
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	Local
Primary Audience	Schools
Results and Impact	I presented my career path to the students to engage them on STEM careers. I had a good level of discussion and many questions.
Year(s) Of Engagement Activity	2018

Description	Laboratory Science Animal association (LASA) meeting in Birmingham, Invited speaker
Form Of Engagement Activity	A formal working group, expert panel or dialogue
Part Of Official Scheme?	No
Geographic Reach	National
Primary Audience	Professional Practitioners
Results and Impact	Gave a presentation on the tolls we have developed to study swine influenza
Year(s) Of Engagement Activity	2018
URL	http://www.lasa.co.uk/meetings/

Description	Lecture given to University of Surrey MSc Veterinary Microbiology students
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	Local
Primary Audience	Postgraduate students
Results and Impact	A 2-hour teaching lecture given to 10 MSc Veterinary Microbiology students at the University of Surrey on 8th December (Lara Harrup)
Year(s) Of Engagement Activity	2017

Description	Lecture of dynamics of vector-borne diseases
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	Local
Primary Audience	Undergraduate students
Results and Impact	Lecture on the dynamics of vector-borne diseases as part of Part II Pathology: Dynamics of Infectious Diseases course at Univerity of Cambridge
Year(s) Of Engagement Activity	2008,2009,2010,2011,2012

Description	Lecture on dynamics of infectious diseases
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	Local
Primary Audience	Postgraduate students
Results and Impact	Lecture as part of MSc in Veterinary Microbiology at University of Surrey
Year(s) Of Engagement Activity	2012,2013,2014,2015,2016

Description	Lecture on entereic coronaviruses to MSc Students (Surrey)
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	Regional
Primary Audience	Postgraduate students
Results and Impact	A lecture was presented on the pathology, diagnosis and detection of enteric coronaviruses, including virus replication and interaction with the host. The students were engaged and asked/answered question throughout the session. We were invited to repeat the lecture for the subsequent student intakes.
Year(s) Of Engagement Activity	2015,2016,2018

Description	MMC2017 Learning Zone seminar
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Public/other audiences
Results and Impact	Presented a seminar on preparation of samples for electron microscopy to 24 MMC2017 delegates. Questions followed asking for advice on specific examples, also advice on interpretation of data and how to improve results.
Year(s) Of Engagement Activity	2012,2015,2017

Description	MSc student visit
Form Of Engagement Activity	Participation in an open day or visit at my research institution
Part Of Official Scheme?	No
Geographic Reach	Regional
Primary Audience	Postgraduate students
Results and Impact	I hosted several MSc students from University of Surrey, discussed avian research with them and demonstrated some laboratory techniques. The students gained an understanding of academic research and laboratory work.
Year(s) Of Engagement Activity	2018,2019

Description	Malaysia workshop
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Professional Practitioners
Results and Impact	Organised and participated in workshop on genetic control of mosquitoes, primarily focusing on refractory methods. Included a set of open lectures from international speakers at University of Malaysia and more focus workshop-style meetings. Several reported changes to research activities and also opinions, also follow-up meeting organized in Glasgow later in 2017
Year(s) Of Engagement Activity	2017

Description	Mapping antigenic determinants of H9N2 avian influenza viruses and improving vaccines and diagnostics" presented at Foreign Talent Lecture: New strategies and new techniques for the prevention and control of major avian diseases. Shandong Binzhou Animal Science & Veterinary Medicine Academy, Binzhou, China. 6th August 2018.
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Professional Practitioners
Results and Impact	The focus of the meeting was to improve the control systems against poultry diseases including development of improved vaccines and diagnostics
Year(s) Of Engagement Activity	2018

Description	Medical and Veterinary Virus Research Symposium (MVVR) Kenya
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Professional Practitioners
Results and Impact	Talk at international conference on One Health research in Kenya
Year(s) Of Engagement Activity	2017

Description	Microbiology Society Annual conference (UK)
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Industry/Business
Results and Impact	Poster or oral presentation at Microbiology Society annual conference, a major international event. The participants included researchers from many different areas of science. This provides a platform to promote research to a wider audience as well as discuss the key findings with experts in my own field.
Year(s) Of Engagement Activity	2010,2011,2012,2013,2014

Description	Microbiology Society Avian Focus Meeting (UK)
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Industry/Business
Results and Impact	I gave a 15 minute oral presentation to an audience of around 80 people in 2016 and 2018. The audience consisted of researchers from many different scientific institutions from different areas of avian research. This was a great opportunity to present to experts in this field and gain novel insights into the project and the possible applications of my work.
Year(s) Of Engagement Activity	2016,2018

Description	Microbiology Today article
Form Of Engagement Activity	A magazine, newsletter or online publication
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Professional Practitioners
Results and Impact	Invited article titled 'How to Image an Exotic Virus' in special Imaging issue (February 2018).
Year(s) Of Engagement Activity	2018

Description	Microsoc 2018 "Structure-guided identification of a pathogen with pandemic potential" poster
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	National
Primary Audience	Professional Practitioners
Results and Impact	Presented a poster on our research at the MicroSoc annual meeting
Year(s) Of Engagement Activity	2018

Description	Negative Strand Virus 2018 Meeting "Structure-guided identification of morbilliviruses with zoonotic potential" Poster
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Postgraduate students
Results and Impact	Presented a poster on the Structure-guided identification of morbilliviruses with zoonotic potential at the international negative strand virus (NSV) meeting in Verona, Italy.
Year(s) Of Engagement Activity	2018

Description	Negative Strand Virus Meeting 2018 "Creation of a high throughput RSV fusion assay for screening inhibitors" Poster
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Professional Practitioners
Results and Impact	Presented a poster on the development of fusion assays for analysis of RSV replication and identification of inhibitors at the international negative strand virus (NSV) meeting in Verona, Italy.
Year(s) Of Engagement Activity	2018

Description	Networking presentation-INRA, Paris, France
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Professional Practitioners
Results and Impact	A presentation at INRA describing the role of the SH protein of BRSV in modulating immune responses in calves. This presentation resulted in a heated debate on the role of viral proteins in immune evasion and their activities in attenuated vaccines.
Year(s) Of Engagement Activity	2017

Description	Newton Agham Researcher Links Workshop on "Novel Vaccines and Diagnostic Technologies Against Emerging and Re-emerging Veterinary Pathogens, Rizal Park Hotel, Manila, Philippines 4-7 February 2019
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Professional Practitioners
Results and Impact	The primary aim of this workshop was to forge long-term research partnerships between early-career researchers, livestock industry and national disease control authorities in the UK and the Philippines. The workshop provided information on emerging and next generation diagnostic and vaccine strategies and their utilization to reduce the impact of viral diseases on livestock and poultry.
Year(s) Of Engagement Activity	2019
URL	https://www.slideshare.net/zerep_cire/2019-newton-agham-researcher-links-workshop-vaccines-and-diagn...

Description	Nick Rufford, Sunday Time Mag, published 27/08/2017
Form Of Engagement Activity	A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme?	No
Geographic Reach	National
Primary Audience	Media (as a channel to the public)
Results and Impact	Provided information and quotes for Sunday TImes Magazine article on GM mosquitoes and Zika
Year(s) Of Engagement Activity	2017
URL	https://www.thetimes.co.uk/article/how-genetically-engineered-mosquitoes-will-save-lives-kwc8nmwh5

Description	Nidovirus symposium
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Other audiences
Results and Impact	I presented my work at the Nidovirus Symposium, which is held every three years and brings together researchers from industry and academia from many different countries. I had some interesting discussions with other researchers and built my network.
Year(s) Of Engagement Activity	2017

Description	Oral presentation at the Microbiology Society annual meeting
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Professional Practitioners
Results and Impact	An oral presentation on Inhibition of Semliki Forest Virus Replication Through Disruption of Lipid Homeostasis was delivered. The audience showed high interest and a good discussion and exchange of ideas followed the presentation.
Year(s) Of Engagement Activity	2017

Description	Oral presentation to Large Animal Research Network 24th May 2017
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	National
Primary Audience	Other audiences
Results and Impact	Talk about work understanding PB1-F2 function in avian hosts highlighting how the animal work carried out was undertaken and monitored. this was delivered to an audience of researchers, funders and industry interested in animal model systems for research and the 3Rs commitment surrounding this work. Discussions with interested parties about our chicken work at the Institute and the diverse way that the birds are entertained whilst being housed (e.g. live food, vegetables, perches, dust baths etc).
Year(s) Of Engagement Activity	2017

Description	Organisation of UK-India Bioinformatics Workshop
Form Of Engagement Activity	A formal working group, expert panel or dialogue
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Professional Practitioners
Results and Impact	The meeting discussed the challenges and opportunities and have decided to come up with a strategy document on working together between UK and India on data-driven biology
Year(s) Of Engagement Activity	2018

Description	Organised the 3rd UK-China CERAD meeting in Guildford, UK in July 2017
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Professional Practitioners
Results and Impact	The 3rd UK-China CERAD meeting helped to bring together professional from both countries to identify challenges and exploit the opportunities for improved control of poultry diseases and increasing food security.
Year(s) Of Engagement Activity	2017
URL	http://www.uk-china-cerad.org/

Description	Organiser of the Animal Genetics and Diseases conference 08 - 10 May 2019 Wellcome Genome Campus, UK. Highlighting recent advances in animal genetics and genomic technologies.
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Professional Practitioners
Results and Impact	I am on the organising committee for this conference. The second conference in this series will highlight recent advances in animal genetics and genomic technologies. It will bring together specialists working on the interface of genomics, genetic engineering and infectious disease with the aims of improving animal and human health and welfare. Novel genomic technologies, mathematical modelling and quantitative genetics approaches, applied to host animals, as well as their pathogens, have transformed the understanding of animal diseases, host-pathogen interactions and epidemiology and their effects on productivity of farmed animal species and food supply chains. This year's conference will not only put the spotlight on the immune response of host animals and epidemiology but also cover the genetics and genomics of pathogens and the impact of animal-human relationships. We encourage registrations from researchers, breeders and technical specialists interested in learning and disseminating the latest cutting-edge techniques and methodologies across model species, wildlife, farmed animals and companion animals.
Year(s) Of Engagement Activity	2019
URL	https://coursesandconferences.wellcomegenomecampus.org/our-events/animal-genetics-diseases-2019/?dm_...

Description	Organiser/invited speaker - PhD course in EM, University of Copenhagen
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Postgraduate students
Results and Impact	Workshop for delegates including lectures, seminars and practical sessions. Outcomes are that delegates are able to make informed decisions regarding microscopy techniques about their future projects, and perform sample preparation and operate instruments. Delegates could ask me for advice regarding their projects which sparked questions and discussion within the group on a wide range of multi-disciplinary subjects.
Year(s) Of Engagement Activity	2018

Description	Organising and giving Keynote talk at the GARAD meeting
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Professional Practitioners
Results and Impact	Global Alliance of Poultry industry professional and academia to discuss about the advances in control strategies of diseases
Year(s) Of Engagement Activity	2018
URL	http://Garad.org

Description	Outreach at TeenTech Events
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	Regional
Primary Audience	Schools
Results and Impact	TeenTech runs lively initiatives with a supporting Award scheme to help young teenagers see the wide range of career possibilities in Science, Engineering and Technology. They work collaboratively with companies, Universities, business organisations and education business partnerships to build sustainable and imaginative programmes focussing on regions of greater social need in the UK and Europe. This outreach actvity involved primarily school children aged 12/13. Several hundred attended and our attendees (Laura Tugwell) spoke with about 30-40 children/teachers.
Year(s) Of Engagement Activity	2017
URL	http://www.teentech.com/

Description	Oxford DTP students
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	Regional
Primary Audience	Postgraduate students
Results and Impact	High level overview of diseases studied [at Pirbright], and the importance of our work, provided to a group of over 30 Oxford DTP students, followed by a networking lunch (29th September 2017).
Year(s) Of Engagement Activity	2017

Description	PPR-Global Research and Expertise Network talk "Examining the zoonotic potential of PPRV" Vienna 2018
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Policymakers/politicians
Results and Impact	Gave a talk to the inaugural meeting of the PPR Global Research and Expertise Network (PPR GREN) at IAEA headquarters in Vienna, Austria. Participants included policy makers from the FAO and OIE.
Year(s) Of Engagement Activity	2018

Description	Pandemic Live (Cheltenham Science Festival)
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	National
Primary Audience	Public/other audiences
Results and Impact	Approximately 250 attendees at a 1 hour discussion around a simulated virus outbreak scenario (Speakers Fry; Carpenter; Beard; Hatchett). Followed by question and answer session.
Year(s) Of Engagement Activity	2018
URL	https://www.cheltenhamfestivals.com/science/whats-on/2018/pandemic-live/

Description	Participant in IDEAS arboviruses workshop
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Professional Practitioners
Results and Impact	Participated in discussions and breakout groups, formed contacts. Planned future meetings on minimum reporting standards in insect infection studies (now funded and scheduled for 2018).
Year(s) Of Engagement Activity	2017
URL	http://ideas.princeton.edu/workshop-for-2017/

Description	Participation in student day at the Pirbright Institute
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	Regional
Primary Audience	Postgraduate students
Results and Impact	Student open day was participated by postgraduate students registered at several Universities, and scientists from Pirbright institute. Students were given a chance to discuss their research activities and future plans with senior scientists. Due to high interest from the students, the institute has decided to organize another open day.
Year(s) Of Engagement Activity	2016

Description	PhD course in Electron Microscopy
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Postgraduate students
Results and Impact	Intensive workshop containing lectures and practicals in the use of electron microscopy in life sciences. This takes place every year at the University of Copenhagen, I am a course organizer and invited speaker.
Year(s) Of Engagement Activity	2012,2013,2014,2015,2016

Description	Pig and Poultry Fair 2019
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	National
Primary Audience	Industry/Business
Results and Impact	Discussed Institute research with pig and poultry farmers, related industry and the general public. Received requests for more information related to research, business and studentships.
Year(s) Of Engagement Activity	2018
URL	https://www.pigandpoultry.org.uk/

Description	Pirbright Pride day
Form Of Engagement Activity	Participation in an open day or visit at my research institution
Part Of Official Scheme?	No
Geographic Reach	National
Primary Audience	Industry/Business
Results and Impact	Set up a stand at the described our research, engaged with policymakers, industry and participants from other organizations and distributed leaflets.
Year(s) Of Engagement Activity	2015

Description	Plenary speaker - Electron Microscopy Cryo-techniques as Tools for Elucidating Biological Processes, University of Minas Gerais, Brazil
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Professional Practitioners
Results and Impact	I was personally invited to talk about imaging the cell biology of host-virus interactions. I was available throughout the associated workshop to provide advice on delegates projects relating to microscopy. These talks sparked discussions and more questions. I was able to increase my network of connections further during this visit to Brazil.
Year(s) Of Engagement Activity	2018

Description	Population Biology of Vector-Borne Diseases Symposium
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Professional Practitioners
Results and Impact	Presentation on "Population biology of Culicoides-borne viruses in Europe" at symposium held at University of Georgia Athens on 24 February 2018
Year(s) Of Engagement Activity	2018

Description	Poster presentation in the IMAV focused meeting on Arboviruses
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Professional Practitioners
Results and Impact	A poster on Inhibition of Semliki Forest Virus Replication Through Disruption of Lipid Homeostasis was presented at the IMAV meeting. The poster generated a lot of discussion and interest.
Year(s) Of Engagement Activity	2017

Description	Poultry Health course
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Professional Practitioners
Results and Impact	Poultry Health Course is run as a residential and online course to transfer recent advances in avian disease research from the laboratory to the field.
Year(s) Of Engagement Activity	Pre-2006
URL	http://www.poultryhealthcourse.com/

Description	Practical training given to LSHTM MSc students
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	Local
Primary Audience	Postgraduate students
Results and Impact	Two-hour practical teaching session given to 12 MSc students at the London School of Hygiene and Tropical Medicine (LSHTM) on 17th November 2017 (Lara Harrup).
Year(s) Of Engagement Activity	2017

Description	Presentation and sicussion with members of the CLA group visiting Pirbright Institute
Form Of Engagement Activity	Participation in an open day or visit at my research institution
Part Of Official Scheme?	No
Geographic Reach	Local
Primary Audience	Public/other audiences
Results and Impact	The CLA is the membership organisation for owners of land, property and businesses in rural England and Wales. The CLA came for a visit to the Pirbrigtht Institute and I presented on Bluetongue virus epidemiology, transmission and risk to the UK agriculture followed by a general discussion
Year(s) Of Engagement Activity	2017

Description	Presentation at Avian viruses focussed meeting - Sept 2018 ND
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Other audiences
Results and Impact	Presentation of data as an offered paper at the molecular biology and pathogenesis of avian viruses meeting, Oxford Sept 2018.
Year(s) Of Engagement Activity	2018

Description	Presentation at Global Alliance for Research on Avian Diseases Conference
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Other audiences
Results and Impact	Presentation of my research at the Global Alliance for Research on Avian Diseases Conference in Vietnam to approximately 80 conference participants resulted in discussions about future research directions.
Year(s) Of Engagement Activity	2018

Description	Presentation at Inter-institute Bioimaging networking meeting
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	National
Primary Audience	Other audiences
Results and Impact	An event was held to enable improved collaboration opportunities between scientists working with bioimaging techniques at the different research institutes within the UK. I presented data from the last 10 years describing our work characterising replication organelles induced by IBV, describing the different techniques and approaches we have used and the outlook for the future. The talk aimed to showcase the bioimaging facilities at Pirbright to encourage new collaborations.
Year(s) Of Engagement Activity	2018

Description	Presentation at RIVR meetings
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	National
Primary Audience	Other audiences
Results and Impact	Presentation of my research at Recently Independent Virology Researchers (RIVR) meetings in 2016, 2017, 2018 and 2019. I discussed my work and future collaborations with other virology researchers working in the UK.
Year(s) Of Engagement Activity	2016,2017,2018,2019

Description	Presentation at the Cheltenham Science Festival
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	National
Primary Audience	Public/other audiences
Results and Impact	The Cheltenham Science Festival is one of the UK's leading science festivals. Founded in 2002, the Cheltenham Science Festival has united some of the greatest scientists, writers and thinkers to explore the wonders of the natural world, the complexities of the human mind and the mysteries of science and space. It was an opportunity for inquisitive minds of all ages to find out more about the world of science. The audience included Key Stage 1-3 students on the start of their education which in turn could lead to future careers in science. The main purpose was to engage with various stakeholders from different backgrounds to inform and educate the general public on BBSRC-funded science which is carried out at the Pirbright Institute. During the talk I was able to demonstrate that basic science can have practical implications.
Year(s) Of Engagement Activity	2016
URL	http://www.cheltenhamfestivals.com/science

Description	Presentation by ND at Nidovirus symposium
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Other audiences
Results and Impact	The post-doctoral researcher presented our data at the 14th international Nidovirus symposium. She discussed data and potential collaboration with other scientisits from the field.
Year(s) Of Engagement Activity	2017

Description	Presentation by RH at Nidovirus symposium
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Other audiences
Results and Impact	PhD student presented our data at the 14th international Nidovirus symposium. He discussed results and potential for future collaboration with other scientists in the field.
Year(s) Of Engagement Activity	2017

Description	Presentation to BBSRC Executive Board
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	National
Primary Audience	Other audiences
Results and Impact	I presented our research to the BBSRC Executive Board when they visited The Pirbright Institute in September 2018 and discussed the impacts of our work..
Year(s) Of Engagement Activity	2018

Description	Presentation: BSI AGM: Immune regulation by the SH protein of BRSV
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Professional Practitioners
Results and Impact	Presentation at the BSI's AGM to describe the data generated regarding the role of the SH protein of BRSV in regulating immune responses after natural infections. Initiated a debate about the role of the SH protein of human RSV in immune regulation.
Year(s) Of Engagement Activity	2017

Description	Princess Anne opening Jenner
Form Of Engagement Activity	Participation in an open day or visit at my research institution
Part Of Official Scheme?	No
Geographic Reach	National
Primary Audience	Other audiences
Results and Impact	Princess Anne, entourage, local politicians and dignitaries visited to open new research building. I was one of small number asked to present/demonstrate/describe our research work, which is on genetic modification of mosquitoes to provide new tools with which to help control mosquito-borne viral diseases.
Year(s) Of Engagement Activity	2017

Description	Public Health Entomology Group (LSHTM)
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Professional Practitioners
Results and Impact	Invited lecture on 4th April 2017.
Year(s) Of Engagement Activity	2017

Description	Public engagement organized at Oxford
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	National
Primary Audience	Public/other audiences
Results and Impact	Set up a stand at the event, a museum at Oxford, and discussed and described research activities within the Institute with public. Set up demonstrations from some biological specimens and distributed leaflets and questionnaire.
Year(s) Of Engagement Activity	2015

Description	Reddit AMA (Ask Me Anything) on vector-borne diseases
Form Of Engagement Activity	Engagement focused website, blog or social media channel
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Public/other audiences
Results and Impact	Took part in a Reddit Ask Me Anything (AMA) on vector-borne diseases. AMAs are a question-and-answer format activity where an individual expert or panel of experts answers questions from the public for a specified duration. We received a large number of questions and the parent post received 493 upvotes, indicating an absolute minimum of 493 participants (and very probably more), so I have given the estimated audience as 500+.
Year(s) Of Engagement Activity	2017
URL	https://www.reddit.com/r/science/comments/6he8go/aaas_ama_hi_were_maria_elena_bottazzi_marcia/

Description	Respond to SMC enquiry re gene drives in mice
Form Of Engagement Activity	A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme?	No
Geographic Reach	National
Primary Audience	Media (as a channel to the public)
Results and Impact	Responded to Science Media Centre request for expert comment on a scientific paper regarding gene drives in mice. Quoted in The Independent
Year(s) Of Engagement Activity	2019
URL	https://www.independent.co.uk/news/science/gene-editing-dna-technology-mice-pests-drives-invasive-sp...

Description	Respond to SMC request re Vosshall paper 08 Feb 2019
Form Of Engagement Activity	A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme?	No
Geographic Reach	National
Primary Audience	Media (as a channel to the public)
Results and Impact	Respond to SMC request re scientific paper describing behaviour-altering drugs in mosquitoes
Year(s) Of Engagement Activity	2019

Description	Reverse genetics seminar to MSc students
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	Local
Primary Audience	Postgraduate students
Results and Impact	A seminar was presented describing reverse genetics of viruses, including infectious bronchitis virus and the application of reverse genetics in research. The students were interested and engaged, participating in activities and asking questions. We were invited to present the seminar in subsequent years for new student intakes.
Year(s) Of Engagement Activity	2013,2014,2015,2016,2018

Description	Royal Institution Christmas Lectures Family Day - LA
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	Regional
Primary Audience	Public/other audiences
Results and Impact	Six Pirbright staff/students ran an all-day exhibit on gene editing and mosquitoes at the Royal Institution About 270 participants visited our stand; wide range of comments, questions, discussion and feedback
Year(s) Of Engagement Activity	2019

Description	Royal Institution Christmas Lectures Family Day - SB
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	Regional
Primary Audience	Public/other audiences
Results and Impact	About 270 participants visited our stand; wide range of comments, questions, discussion and feedback
Year(s) Of Engagement Activity	2019

Description	Royal Society of Biology Biology week talk, Winchester, 10/10/2017
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	Regional
Primary Audience	Public/other audiences
Results and Impact	Talk organized by Royal Society of Biology as part of Biology Week. This one in Winchester, audience was general public with strong representation of school-age. Questions and discussion during and after formal talk.
Year(s) Of Engagement Activity	2017

Description	SGM Meeting 2017 Presentation: Regulation of the transcription factor NF=kB by the SH protein of BRSV
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Professional Practitioners
Results and Impact	Presentation at a workshop of the Society for General Microbiology on the role of the SH protein of BRSV in regulating the transcription factor NF-kB in bovine antigen presenting cells. Many questions about protein-protein interactions were generated. Good feedback on our work.
Year(s) Of Engagement Activity	2017

Description	SMBE 2018 meeting
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Professional Practitioners
Results and Impact	Presentation entitled "Phylogenetically young genes and their function in Anopheles mosquitoes" given during the Society for Molecular Biology and Evolution 2018 meeting (8-12 July 2018) in Yokohama, Japan. Discussions held with potential collaborators on joined future research projects.
Year(s) Of Engagement Activity	2018
URL	https://www.smbe2018.jp/

Description	STEM Day - Speed interviews and Careers Day 2018 - BA
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	Local
Primary Audience	Schools
Results and Impact	Stimulating increased interest in research
Year(s) Of Engagement Activity	2018

Description	Scheduled Twitter session with University of Nottingham students on professional skills for biosciences
Form Of Engagement Activity	Engagement focused website, blog or social media channel
Part Of Official Scheme?	No
Geographic Reach	Regional
Primary Audience	Undergraduate students
Results and Impact	Hour-long Twitter chat on Friday 12th May with biotechnology students, microbiology students, animal science students interested in animal diseases and animal welfare and behaviours and agriculture students interested in livestock.
Year(s) Of Engagement Activity	2017
URL	https://twitter.com/search?q=%23biosciprofskills

Description	School visit
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	Local
Primary Audience	Schools
Results and Impact	Met and discussed A level students in small groups (3 students at the time) and discussed different aspects of my work as a scientist.
Year(s) Of Engagement Activity	2015

Description	School visit
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	Local
Primary Audience	Schools
Results and Impact	Gave a presentation to a group of students about research performed at the Pirbright Institute and discussed different aspects of scientists' work.
Year(s) Of Engagement Activity	2017

Description	School visit (Farnborough)
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	Local
Primary Audience	Schools
Results and Impact	Gave a presentation to a group of students about research performed at the Pirbright Institute and discussed various aspects of scientists' work.
Year(s) Of Engagement Activity	2018

Description	Scientific Presentation: SOVE lecture
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Professional Practitioners
Results and Impact	Society of Vector Ecology Presentation 'The Gnatwork'. 6th October 2017 over 150 attendance.
Year(s) Of Engagement Activity	2017
URL	http://www.uibcongres.org/sove2017/

Description	Site tour with Department of Trade and Industry
Form Of Engagement Activity	Participation in an open day or visit at my research institution
Part Of Official Scheme?	No
Geographic Reach	National
Primary Audience	Policymakers/politicians
Results and Impact	Tour/site visit with Department of Trade and Industry (25 people attending)
Year(s) Of Engagement Activity	2017

Description	Site tour with UK Science Partnership for Animal and Plant Health, BBSRC (Chris Jacobs, Julie Fitzpatrick)
Form Of Engagement Activity	Participation in an open day or visit at my research institution
Part Of Official Scheme?	No
Geographic Reach	National
Primary Audience	Policymakers/politicians
Results and Impact	'Fact-finding' visit from Chris Jacobs (Head of Secretariat, UK Science Partnership for Animal and Plant Health) & Julie Fitzpatrick (Chair of the Partnership) to organisations involved in the areas of animal and plant health science capabilities, to gain a better understanding of who the key players are, what they're working on, and any particular issues the Partnership may be able to address.
Year(s) Of Engagement Activity	2017

Description	St Andrew's Catholic School visit to Pirbright
Form Of Engagement Activity	Participation in an open day or visit at my research institution
Part Of Official Scheme?	No
Geographic Reach	Regional
Primary Audience	Schools
Results and Impact	College students visited the Institute to learn about lab designs. They were given talks about the equipment and labs and then toured around the labs at Pirbright. Students were highly interested about our facilities and the work we are conducting at The Institute.
Year(s) Of Engagement Activity	2016

Description	Stacey Human - Pirbright Village Fair
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	Local
Primary Audience	Public/other audiences
Results and Impact	The postdoctoral research Stacey Human in my team attended The Pirbright Village Duck Fair - a local event in our community supported by the Institute where the work of the Institute was advertised.
Year(s) Of Engagement Activity	2018

Description	Stacey Human - University of Surrey Open Day
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	Regional
Primary Audience	Schools
Results and Impact	The postdoctoral researcher in my team took part in the University of Surrey Open Day advertising the work of The Pirbright Institute
Year(s) Of Engagement Activity	2018

Description	Surrey County Show
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	National
Primary Audience	Public/other audiences
Results and Impact	The Surrey County Show is an agricultural show in Guildford that is visited by the general public. The Pirbright Institute had a stand with various activities for visitors to take part in and explain our science to a wide audience. Giulia spent a day working on the stand and spoke with many children and adults about her work at Pirbright to change public attitudes and stimulate increased interest in research.
Year(s) Of Engagement Activity	2018

Description	Surrey County Show
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	Regional
Primary Audience	Public/other audiences
Results and Impact	Stall at Surrey County Show, engage with the public where they can ask any questions they have about the work we do. This sparks discussions, and an increase in knowledge of the general public. I invited the Lord Lieutenant of Surrey to our stand, with whom I have built a solid relationship. This has lead to engagement of his office with the Institute, for example the visit of HRH Princess Anne to Pirbright in 2017.
Year(s) Of Engagement Activity	2018

Description	Surrey County Show (Guildford)
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	Regional
Primary Audience	Public/other audiences
Results and Impact	Stall at Surrey County show attended by Jo Stoner (Research Technician). Gave commentary on the threat of viruses to UK livestock species.
Year(s) Of Engagement Activity	2017
URL	http://surreycountyshow.co.uk/

Description	Surrey Open day
Form Of Engagement Activity	Participation in an open day or visit at my research institution
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Undergraduate students
Results and Impact	Described the research activity of the Pirbright Institute for students from school, graduate and postgraduate students, and provided information on research facilities in the institute.
Year(s) Of Engagement Activity	2017
URL	https://www.surrey.ac.uk/opendays/vet

Description	Surrey vet school open day - MJB
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	National
Primary Audience	Other audiences
Results and Impact	The event was an open day for prospective students for the University of Surrey vet school. The course includes a research placement, which can be conducted at Pirbright. Therefore there was a stand from Pirbright to describe the research we perform generally as well as a discussion of our specific research. The audience were generally interested and the information helped inform them when selecting a university course.
Year(s) Of Engagement Activity	2018

Description	Taiwan -UK Partnering Award: Surveillance of influenza viruses
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Professional Practitioners
Results and Impact	Attended the International Flu Virus Symposium for Celebrating IPM 47th Anniversary" in Tri-Service General Hospital in Taipei, Taiwan. Tri-Service General Hospital address: No. 325, Sec. 2, Chenggong Rd., Neihu Dist., Taipei City 114, Taiwan (R.O.C.) Invited speaker for medical staff and research scientists.
Year(s) Of Engagement Activity	2019
URL	https://www.cdc.gov.tw/rwd/english

Description	Talk as part of the University of Veterinary Medicine (Vienna) doctoral school "Infectious Diseases of Pig and Poultry"
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Policymakers/politicians
Results and Impact	Talked about the Pirbright Institute's work on African swine fever virus to researches in Veterinary Medicine (Virology and Immunology) in Vienna.
Year(s) Of Engagement Activity	2018

Description	Talk at Avian viruses focussed meeting - Sept 2018 HM
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Other audiences
Results and Impact	Presentation of data as an offered paper at the Molecular biology and pathogenesis of avian viruses meeting in Oxford, September 2018.
Year(s) Of Engagement Activity	2018

Description	Talk to Agrifood and Biosciences Institute, Northern Ireland
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	Regional
Primary Audience	Professional Practitioners
Results and Impact	Formal presentation on Culicoides-borne arboviruses with discussion of impact in Northern Ireland
Year(s) Of Engagement Activity	2017

Description	Teaching- lecturing, small group classroom teaching and laboratory practical teaching
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	National
Primary Audience	Postgraduate students
Results and Impact	I have been actively engaged with teaching activities at the University of Surrey, giving lectures, small group classes and laboratory practical classes to vet students and MSc students.
Year(s) Of Engagement Activity	2015,2016,2017

Description	Teentech
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	Local
Primary Audience	Schools
Results and Impact	The annual Teentech student science event in Guildford aims to encourage teenagers to consider science subjects and science careers. Our stand had considerable interest from the schoolchildren who were very enthusiastic. The event stimulated an increased interest in science and research.
Year(s) Of Engagement Activity	2016,2019

Description	Teentech Guildford - ND
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	Local
Primary Audience	Schools
Results and Impact	Teentech is an event held for students from a range of local school who have an interest in science to experience the range of scientific activities that could be open to them for future careers. Students in small groups rotate around stands at the event to perform activities at each station, guided by scientists. The Pirbright stand was demonstrating the principle of genetic modification of mosquitos. The children were very interested in the exhibit and asked lots of questions.
Year(s) Of Engagement Activity	2013,2019

Description	The Hurst careers event - HJM
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	Local
Primary Audience	Schools
Results and Impact	The Hurst school held a careers event for GCSE students to attend throughout the day. The Pirbright stand contained a variety of engagement activities to demonstrate the research we perform and to stimulate discussion. The students seemed interested in the activities and those more interested in a science career asked a variety of questions about the research and entry into scientific research as a career.
Year(s) Of Engagement Activity	2018

Description	The Pirbright Village Duck Fair held on 16 June 2018
Form Of Engagement Activity	Participation in an open day or visit at my research institution
Part Of Official Scheme?	No
Geographic Reach	Local
Primary Audience	Public/other audiences
Results and Impact	Local Village Fair, interaction with local residence to expanse research aims and activities of The Pirbright Institute and benefits to UK and around the globe to prevent and control infectious diseases of animals and humans.
Year(s) Of Engagement Activity	2018

Description	This Week In Virology Podcast 532: Morbillivirus had a little lamb
Form Of Engagement Activity	A broadcast e.g. TV/radio/film/podcast (other than news/press)
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Professional Practitioners
Results and Impact	Our research paper on morbillivirus host-range in Journal of Virology was highlighted for in-depth discussion on this episode of TWIV where they spent 30+ minutes talking about the work and its potential impact. This podcast is the number one podcast in virology and listened to across the globe.
Year(s) Of Engagement Activity	2019
URL	http://www.microbe.tv/twiv/twiv-532/

Description	This week in Virology: 533: Recurring threads
Form Of Engagement Activity	A broadcast e.g. TV/radio/film/podcast (other than news/press)
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Professional Practitioners
Results and Impact	Following our paper being highlighted on the previous edition of TWIV I wrote a letter to further the discussion of this subject and clarify some technical points on The Pirbright Institute. This was read out as part of the podcast and discussed.
Year(s) Of Engagement Activity	2019
URL	http://www.microbe.tv/twiv/twiv-533/

Description	Tillingbourne Bug Hunt
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	Local
Primary Audience	Public/other audiences
Results and Impact	Local entomology event for general public - changes in public attitudes and stimulating increased interest in insects/entomology and research
Year(s) Of Engagement Activity	2018

Description	Tillingbourne School bug hunt - ND
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	Local
Primary Audience	Schools
Results and Impact	Primary school students were involved in an event to identify insects in the school grounds.
Year(s) Of Engagement Activity	2018

Description	Tillingbourne School science event - ND
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	Local
Primary Audience	Schools
Results and Impact	Tillingbourne School held a science event for year 3 students to explore different aspects of science including smell, acid & alkali, magnetism etc. Nicole was based at the microscopy station helping children to view samples under the microscope, explain what they were looking at and answer any questions to children asked. The children were highly engaged and interested in the exhibit.
Year(s) Of Engagement Activity	2018

Description	Tillingbourne Year 3 Science Day 2018 - EL
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	Local
Primary Audience	Schools
Results and Impact	Stimulating increased interest in research
Year(s) Of Engagement Activity	2018

Description	Toolbox workshop at IVVN Stirling 2018
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Other audiences
Results and Impact	A second workshop held as a sattelite meeting to the International Veterinary Vaccinology Conference in Stirling to decide on community priorities for future immunological tools to promote veterinary vaccine development.
Year(s) Of Engagement Activity	2012,2018

Description	Tour of Philip Mellor Insectary (BBSRC)
Form Of Engagement Activity	Participation in an open day or visit at my research institution
Part Of Official Scheme?	No
Geographic Reach	National
Primary Audience	Supporters
Results and Impact	BBSRC tour of Philip Mellor Insectary on 4th December 2017 (Jo Stoner).
Year(s) Of Engagement Activity	2017

Description	Tour of Philip Mellor Insectary (Country Land and Business Association)
Form Of Engagement Activity	Participation in an open day or visit at my research institution
Part Of Official Scheme?	No
Geographic Reach	National
Primary Audience	Third sector organisations
Results and Impact	Tour of Philip Mellor Insectary with Country Land and Business Association (20 people)
Year(s) Of Engagement Activity	2017

Description	Tour of Pirbright Research facilities for CLA group
Form Of Engagement Activity	Participation in an open day or visit at my research institution
Part Of Official Scheme?	No
Geographic Reach	Local
Primary Audience	Public/other audiences
Results and Impact	The CLA is the membership organisation for owners of land, property and businesses in rural England and Wales. The CLA came for a tour of the Jenner building facilities at The Pirbright Institute. I led a tour of our building for the group and described the work undertaken by the avian research programme here.
Year(s) Of Engagement Activity	2017

Description	U3A talk
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	Local
Primary Audience	Public/other audiences
Results and Impact	Presentation on influenza virus research at The Pirbright Institute for the local University of the Third Age group, approx. 50 people aged 65 years + all with an interest in science.
Year(s) Of Engagement Activity	2018
URL	https://cu3asandt.wordpress.com/2018/09/23/meeting-2nd-october/

Description	UK vector meeting 2018 (Organising committee; Chair) (December; John Innes Centre, UK)
Form Of Engagement Activity	A formal working group, expert panel or dialogue
Part Of Official Scheme?	No
Geographic Reach	National
Primary Audience	Professional Practitioners
Results and Impact	Chaired and helped organise meeting on UK Vector-borne Diseases as part of committee. Included reviewing talks and inviting speakers.
Year(s) Of Engagement Activity	2018
URL	http://www.hpruezi.nihr.ac.uk/media/29013/master-draft-vbd-programme-with-titles-oct18_.pdf

Description	UK-China Philippines-Thailand Swine and Poultry Research Initiative project kick-off workshop" at China Agriculture University, Beijing, 26th-28th September 2018.
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Professional Practitioners
Results and Impact	Emerging new approaches were discussed for prevention and control of infectious diseases affecting swines and poultry.
Year(s) Of Engagement Activity	2018

Description	Understanding Antigenic Diversity, Zoonotic potential and immunological prevention of avian influenza viruses affecting poultry. Presented at "UK-China Philippines-Thailand Swine and Poultry Research Initiative project kick-off workshop" at China Agriculture University, Beijing, 26th-28th September 2018.
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Professional Practitioners
Results and Impact	Meeting focus was to share research plans funded under "UK-China-Philippines-Thailand Swine and Poultry Research Initiative". Research projects investigate pathogens, zoonotic pathogens or "commensals" associated with food safety issues affecting poultry and swine.
Year(s) Of Engagement Activity	2018
URL	https://bbsrc.ukri.org/funding/filter/joint-swine-poultry-initiative/

Description	University of California Davis School of Veterinary Medicine
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Postgraduate students
Results and Impact	External Meeting at University of California Davis School of Veterinary Medicine, 16-18th October 2017. Talk given (Lara Harrup) on 'Tools and Resources for Arbovirus Epidemiology in the Genomic Era'. Meeting organised as part of the University of Liverpool BBSRC US Partnering Award: Vector-borne diseases in the UK & US: common threats and shared solutions (BB/N021916/1).
Year(s) Of Engagement Activity	2017

Description	University of Surrey Open Day
Form Of Engagement Activity	Participation in an open day or visit at my research institution
Part Of Official Scheme?	No
Geographic Reach	Regional
Primary Audience	Public/other audiences
Results and Impact	I represented The Pirbright Institute at the University of Surrey Vet School open day, talking about my research and what it is like to be a research scientist.
Year(s) Of Engagement Activity	2015

Description	University of Surrey Vet School Open Day
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	National
Primary Audience	Schools
Results and Impact	Institute stand at Surrey Vet School - introducing the institute to prospective vet students
Year(s) Of Engagement Activity	2018

Description	Vet School Open Day
Form Of Engagement Activity	Participation in an open day or visit at my research institution
Part Of Official Scheme?	No
Geographic Reach	National
Primary Audience	Schools
Results and Impact	University of Surrey School of Veterinary Medicine Open Day - introduced The Pirbright Institute and the research we conduct to prospective students and parents.
Year(s) Of Engagement Activity	2018

Description	Vet Times
Form Of Engagement Activity	A magazine, newsletter or online publication
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Professional Practitioners
Results and Impact	Information on research activity of Avian Immunology group at the Pirbright Institute
Year(s) Of Engagement Activity	2018

Description	VetVac toolbox kick off meeting, Pirbright
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Other audiences
Results and Impact	Organsised a workshop part sponsored by the BBSRC Veterinary Vaccinology Network that bought key international leaders in veterinary immune reagents together to discuss lessons from the past and how we should progress in the future, reaching as wide an audience as possible.
Year(s) Of Engagement Activity	2017

Description	Veterinary Research Club
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	Regional
Primary Audience	Professional Practitioners
Results and Impact	Presentation of the data generated and its importance in understanding immune responses to vaccination and natural infections. About 50 veterinarians and researchers working in veterinary sciences attended the one-day meeting, which initiated discussions about the possibility of generating new vaccines against BRSV.
Year(s) Of Engagement Activity	2017

Description	Veterinary Times article on morbillivirus vaccines.
Form Of Engagement Activity	A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme?	No
Geographic Reach	National
Primary Audience	Professional Practitioners
Results and Impact	I was interviewed by a journalist from The Veterinary Times about our recent Journal of Virology research paper on morbillivirus host-range. This was then written up for the next edition of this publication.
Year(s) Of Engagement Activity	2019
URL	https://www.vettimes.co.uk/news/scientists-multi-purpose-ppr-vaccine-closer-to-completion/

Description	Virtual video tour of the animal facilities in Pirbright
Form Of Engagement Activity	Engagement focused website, blog or social media channel
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Public/other audiences
Results and Impact	Contributed to public understanding of animal research by being interviewed on the virtual video tour of the animal facilities in Pirbright - one of only four such initiatives in the UK.
Year(s) Of Engagement Activity	2017

Description	Volunteered at a school open day
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	Local
Primary Audience	Schools
Results and Impact	I represented The Pirbright Institute talking at a school about my research and what it is like to be a research scientist.
Year(s) Of Engagement Activity	2015

Description	Winston Churchill Careers Fair. Winston Churchill School, Hermitage Road, St Johns, Woking, Surrey, GU21 8TL
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	Local
Primary Audience	Schools
Results and Impact	AS attended and presented at this event to stimulate increased interest in science and research.
Year(s) Of Engagement Activity	2018

Description	Workshop with MSc students in Veterinary Microbiology
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	Regional
Primary Audience	Postgraduate students
Results and Impact	Both PI and postdoctoral scientists employed on projects participated in a 1 week workshop of MSc students from the University of Surrey including training activities within the high containment facilities
Year(s) Of Engagement Activity	2017,2018,2019

Description	World Zoonosis Day 2018 - web story
Form Of Engagement Activity	A magazine, newsletter or online publication
Part Of Official Scheme?	No
Geographic Reach	National
Primary Audience	Professional Practitioners
Results and Impact	Contributed to an article from our institute on World Zoonosis Day highlighting the importance of research in this area.
Year(s) Of Engagement Activity	2018
URL	https://www.pirbright.ac.uk/news/2018/07/pirbright-scientists-are-developing-vaccine-candidates-anim...

Description	a talk at the Recently Independent Virology Investigators meeting
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	National
Primary Audience	Professional Practitioners
Results and Impact	I gave a talks outlining my current research and future plans.
Year(s) Of Engagement Activity	2015,2016,2017

Description	email interview with journalist (Sukanya Charuchandra, The Wire)
Form Of Engagement Activity	A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Media (as a channel to the public)
Results and Impact	Email interview for journalist for "The Wire" - an online technology-focused media outlet. Helped provide context and impact for the resulting piece. https://thewire.in/the-sciences/scientists-identify-protein-that-mosquitoes-need-to-lay-viable-eggs
Year(s) Of Engagement Activity	2019
URL	https://thewire.in/the-sciences/scientists-identify-protein-that-mosquitoes-need-to-lay-viable-eggs

Description	international workshop on "Self-Propagation of Artificial Genetic Elements: Gene Drives, Risks & Tipping Points Bremen 19-20 June 2018
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Policymakers/politicians
Results and Impact	Workshop considering regulatory, social and technical environment for gene drive systems in insects, primarily for German and other EU regulatory and political entities.
Year(s) Of Engagement Activity	2018

Description	organised workshops at Microbiology Society annual conferences 2014 - 2018
Form Of Engagement Activity	Participation in an activity, workshop or similar
Part Of Official Scheme?	No
Geographic Reach	International
Primary Audience	Other audiences
Results and Impact	I co-organised virology division workshops on viral evolution and diversity and RNA viruses with colleagues from the virology division. The workshops were held at the annual Microbiology Society conferences in spring each year. Organisation involved selecting abstracts for oral or poster presentation, ordering the presentations and chairing the sessions.
Year(s) Of Engagement Activity	2015,2016,2017,2018

Description	talk to the U3A
Form Of Engagement Activity	A talk or presentation
Part Of Official Scheme?	No
Geographic Reach	Regional
Primary Audience	Public/other audiences
Results and Impact	I gave a talk to the University of the 3rd Age (U3A)- a group of the general public above the age of 65.
Year(s) Of Engagement Activity	2016