Low Containment

Lead Research Organisation: The Pirbright Institute
Department Name: UNLISTED

Abstract

Low containment experimental facilities support, enhance and extend the range of viruses studied at the Pirbright Institute. The primary infrastructure associated with these facilities is the BBSRC National Centre for Vaccinology, the Jenner Building, which was opened in 2016. This facility houses a wide range of groups working on strategically important endemic and exotic viral pathogens that can be handled under low containment, including Marek’s disease virus, infectious bronchitis virus and low pathogenicity animal influenzas. In addition, the Jenner building also houses groups that study the genetics and genomics of virus hosts, using state of the art technologies. This capability is nationally unique both because it supports studies of viruses not replicated elsewhere in the UK and because of its position in close proximity to High Containment Experimental Facilities in Pirbright.
Our in vivo facilities supporting avian research are housed in a temporary facility. This enables study of internationally important avian viruses within the natural host. During the term of the CCG, new facilities will be constructed on the Pirbright site, enabling expansion of this work. This includes a specified pathogen free (SPF) hatchery and rearing facility due for delivery in early 2018 and refurbishment of current high containment animal facilities to deliver a low containment Poultry Experimental Facility in late 2018.

Organisations

Publications

10 25 50
 
Description The key development within low containment science at The Pirbright Institute is that of providing facilities that allow handling of non-infectious and low pathogenicity agents within laboratories that are safe for the user and environment and with minimal interruptions to work. Within the first two years of reporting, these objectives have been achieved to the standards within the Impact Plan for this project in the following ways: 1) Interruptions to science are within the boundaries set for the facility. 2) Pirbright remains a hub for Avian research as demonstrated by the production of publications and outreach activities. The SPF facility is in the final stages of construction and will enable poultry science in the near future alongside a greater capacity to carry out contained studies. 3) Relationships with the National Avian Research Facility remain open and were reinforced by meetings that included BBSRC (UKRI) members. 4)Bioinformatics capabilities have been strengthened significantly with expansion of computing facilities and staff, the latter provisioned from the Science Services CCG component. 5) Significant progress has been made in opening up the low containment facilities for training and also in provision of outreach activities. 6) Insectary facilities, which have seen a significant rise in demand within the science conducted by Pirbright have been expanded via refurbishment of the IS4L facility followed by a successful bid for refurbishment in 2019. This is a medium term, interim solution and dedicated facilities will be required in the longer term. In total 96 peer-reviewed publications and 322 engagement activities have been enabled by this infrastructure, including a major focus on Avian research and viruses spread by arthropod vectors.
Exploitation Route Building of the Avian SPF facility remains a major component of the Pirbright suite of buildings and will be a major focus of research in this field both for internal and external workers. In addition, the interim insectary facilities will enhance workers abilities to carry out research on exotic and in some cases transgenic arthropods which is key future demand for the site. Keeping pace with the health and safety and biosecurity concerns around these studies will also influence the wider community as evidenced by the close relationship with HSE on guidelines for arthropod containment.
Sectors Agriculture, Food and Drink

 
Description Minimal infrastructure downtime enabled by planned preventative maintenance on the BBSRC National Vaccinology Centre (Jenner Building) has enabled our research to be conducted under expected timescales and provided additional space for external workers. In addition, the provision of interim insectary facilities has provided a medium-term solution to a lack of space, without major disruption to work programmes, although a more permanent solution will be required in the long-term.
First Year Of Impact 2017
Sector Agriculture, Food and Drink
Impact Types Societal,Economic,Policy & public services

 
Description BBSRC 18ALERT panel member
Geographic Reach National 
Policy Influence Type Participation in a advisory committee
Impact Provision of multi-user equipment to core facilities to enable BBSRC remit science. Part of the remit for this call was to allow shared access from other institutions, widening the impact of BBSRC investment.
 
Description BBSRC ALERT18 panel member (invited)
Geographic Reach National 
Policy Influence Type Participation in a advisory committee
Impact Panel for ALERT18 multi-user equipment award by BBSRC
 
Description BBSRC-Newton Fund Agricultural Data Re-use Advisory Group
Geographic Reach National 
Policy Influence Type Participation in a advisory committee
Impact Advice on re-usability of data generated in research projects.
 
Description Coalition for Epidemic Preparedness Innovations Nipah Taskforce Member
Geographic Reach Multiple continents/international 
Policy Influence Type Participation in a advisory committee
 
Description Contributed to Chief Medical Officers report- Genomics section
Geographic Reach Europe 
Policy Influence Type Implementation circular/rapid advice/letter to e.g. Ministry of Health
URL https://assets.publishing.service.gov.uk/government/uploads/system/uploads/attachment_data/file/6310...
 
Description Contribution to a POSTnote on 'Reducing UK Antibiotic Use in Animals'
Geographic Reach National 
Policy Influence Type Participation in a advisory committee
 
Description Human Infection Challenge Models for Vaccination
Geographic Reach Europe 
Policy Influence Type Influenced training of practitioners or researchers
 
Description IUCN Assessment of Synthetic Biology and Biodiversity Conservation
Geographic Reach Multiple continents/international 
Policy Influence Type Participation in a advisory committee
URL https://www.iucn.org/theme/science-and-economics/our-work/other-work/synthetic-biology-and-biodivers...
 
Description IUCN Technical Subgroup on Synthetic Biology and Biodiversity Conservation
Geographic Reach Multiple continents/international 
Policy Influence Type Participation in a advisory committee
 
Description Inter-institute imaging network
Geographic Reach National 
Policy Influence Type Participation in a advisory committee
 
Description Invited member of RCaH Science Advisory Board
Geographic Reach National 
Policy Influence Type Participation in a advisory committee
Impact The Research Complex at Harwell is a UKRI funded building with the remit of enabling researchers across many disciplines to take advantage of the national facilities on the Harwell campus. RCaH is going through significant change at the moment and the advisory board has been asked to contribute to defining its future direction.
 
Description Member of RMS EM Section committee
Geographic Reach Multiple continents/international 
Policy Influence Type Membership of a guideline committee
Impact This committee informs the RMS on which areas to invest funds in regarding training, conferences, outreach events. RMS members are international.
 
Description Member of the Board of the Jenner Vaccine Foundation
Geographic Reach National 
Policy Influence Type Membership of a guideline committee
Impact Helping with the strategic decisions of the Board of the Jenner Vaccine Foundation
 
Description Netherlands Commission on Genetic Modification (COGEM) Project "Gene drives, inventory of previous research and experience"
Geographic Reach Europe 
Policy Influence Type Gave evidence to a government review
 
Description POSTnote Report on Climate and vector-diseases in humans in the UK
Geographic Reach National 
Policy Influence Type Gave evidence to a government review
 
Description PPR Global Research and Expertise Network (PPR GREN) membership (OIE/FAO)
Geographic Reach Multiple continents/international 
Policy Influence Type Participation in a advisory committee
 
Description Publication policy document for core facilities - EM-UK
Geographic Reach Multiple continents/international 
Policy Influence Type Influenced training of practitioners or researchers
Impact I am a founder member of the network created for electron microscopists within UK, EM-UK. The annual meeting is highly productive, and training and managing core facilities is regularly discussed. Recognition of these facilities is essential for their continued existence so we produced a generic document hosted on the RMS website which can be adopted by any core facility in any institution, and describes the publication policy which users had to adhere to. This has already raised the profile of core facilities around the country and is a successful outcome from the EM-UK meetings.
 
Description RCUK Engagement workshop, Cosener's House, 20-21 April 2017
Geographic Reach National 
Policy Influence Type Influenced training of practitioners or researchers
 
Description Royal Microscopical Society Training Focussed Interest Group
Geographic Reach Multiple continents/international 
Policy Influence Type Influenced training of practitioners or researchers
Impact A review of the microscopy training courses available globally has been undertaken. We are in the process of collating and preparing a gap analysis. This will be used to guide RMS policy on training provided in the UK, both UK and international delegates.
 
Description Scientific Advisory Group for Emergencies Exercise (Royal Society: 11/06/19): Examined simulated emergence of arbovirus in the UK with main decision making bodies and personnel.
Geographic Reach National 
Policy Influence Type Gave evidence to a government review
 
Description Training MSc students
Geographic Reach Local/Municipal/Regional 
Policy Influence Type Influenced training of practitioners or researchers
Impact Delivered a new training session to MSc students teaching viral quantification assays. This lead to a greater understanding of laboratory techniques and academic research.
 
Description WHO R&D Blueprint Roadmaps: Consultation on Nipah virus
Geographic Reach Multiple continents/international 
Policy Influence Type Participation in a advisory committee
 
Description A One Health approach to pan-valent morbillivirus vaccines
Amount £773,237 (GBP)
Funding ID BB/R019843/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 05/2018 
End 03/2021
 
Description Active and passive Immunity induced by aerosols
Amount £100,000 (GBP)
Funding ID BB/R506448/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 10/2017 
End 08/2021
 
Description Antigenic characterisation of infectious bursal disease virus to improve vaccination strategies and vaccine design
Amount £450,685 (GBP)
Funding ID BB/S014594/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 07/2019 
End 12/2021
 
Description Assessment of the immunogenicity of bovine herpesvirus 4-based vectors delivering Nipah virus glycoproteins in swine
Amount £41,895 (GBP)
Organisation European Commission 
Sector Public
Country European Union (EU)
Start 02/2019 
End 04/2019
 
Description Aviagen Industry Funding
Amount £422,383 (GBP)
Organisation Aviagen Group 
Sector Private
Country United States
Start 09/2019 
End 12/2020
 
Description BBSRC - responsive mode
Amount £187,370 (GBP)
Funding ID BB/S007911/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 03/2019 
End 03/2022
 
Description BBSRC IAA The Pirbright Institute
Amount £300,000 (GBP)
Funding ID BB/S506680/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 03/2018 
End 03/2021
 
Description BBSRC Newton Fund Swine and Poultry Research Initiative
Amount £865,623 (GBP)
Funding ID BB/R01275X/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 04/2018 
End 06/2021
 
Description British Egg Marketing Board PhD studentship
Amount £76,000 (GBP)
Organisation British Egg Marketing Board 
Sector Private
Country United Kingdom
Start 10/2018 
End 09/2022
 
Description Broad and effective protection against influenza achieved by viral vectored vaccines
Amount £1,607,691 (GBP)
Funding ID MR/S037160/1 
Organisation Medical Research Council (MRC) 
Sector Public
Country United Kingdom
Start 05/2019 
End 04/2022
 
Description Commonwealth Scholarship. Molecular determinants impacting avian influenza H9N2 virus evolution, replication fitness and virulence
Amount £200,000 (GBP)
Funding ID LKCS-2019- 665 
Organisation British Council 
Sector Charity/Non Profit
Country United Kingdom
Start 11/2019 
End 10/2022
 
Description Components of the Medea gene drive system in a mosquito Aedes aegypti
Amount £1,209,348 (GBP)
Funding ID BB/S001964/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 11/2018 
End 10/2021
 
Description DARPA PREEMPT
Amount $2,700,000 (USD)
Organisation Defense Advanced Research Projects Agency (DARPA) 
Sector Public
Country United States
Start 10/2018 
End 03/2022
 
Description Defining the circadian clock in chicken cells and its impact upon viral replication
Amount £4,500 (GBP)
Organisation The Houghton Trust 
Sector Charity/Non Profit
Country United Kingdom
Start 11/2019 
End 11/2020
 
Description Development of novel universal influenza vaccines
Amount £120,000 (GBP)
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 10/2016 
End 10/2020
 
Description Dissecting the molecular pathways of MDV oncoprotein Meq for understanding pathogenesis and aid vaccine development
Amount £440,000 (GBP)
Funding ID BB/R007896/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 04/2018 
End 03/2021
 
Description Enhancing protective efficacy of avian influenza vaccines through targeted delivery of protective antigens to chicken immune cells
Amount £98,212 (GBP)
Funding ID BB/R50595X/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 09/2017 
End 09/2021
 
Description FLI- TPI joint PhD studentships Avian influenza immunology markers.
Amount £100,000 (GBP)
Organisation Friedrich Loeffler Institute 
Sector Academic/University
Country Germany
Start 07/2018 
End 06/2022
 
Description Further development of infectious bronchitis virus vaccines adapted for production in cell culture
Amount £262,701 (GBP)
Funding ID BB/R019576/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 10/2018 
End 03/2020
 
Description Genomia Project Grant
Amount £174,052 (GBP)
Organisation Genomia fund 
Sector Charity/Non Profit
Country United Kingdom
Start 02/2019 
End 01/2020
 
Description Identification and evaluation of swine mAbs in pig influenza challenge model
Amount $599,944 (USD)
Funding ID OPP1201470 
Organisation Bill and Melinda Gates Foundation 
Sector Charity/Non Profit
Country United States
Start 10/2018 
End 10/2019
 
Description Identification of interaction sites between the genomic segments of influenza virus as putative novel anti-viral targets
Amount £100,000 (GBP)
Organisation The Pirbright Institute 
Sector Academic/University
Country United Kingdom
Start 10/2019 
End 09/2024
 
Description In vitro evaluation of peptide-based immune checkpoint inhibitors as potential molecular adjuvants for improved PRRS vaccines
Amount £4,921 (GBP)
Organisation The Pirbright Institute 
Sector Academic/University
Country United Kingdom
Start 08/2019 
End 03/2020
 
Description Internal Seed Fund- Development of an IBDV diagnostic test that can differentiate infected from vaccinated animals, for use in surveillance.
Amount £10,000 (GBP)
Organisation The Pirbright Institute 
Sector Academic/University
Country United Kingdom
Start 12/2019 
End 02/2020
 
Description LVIF
Amount $1,500,000 (CAD)
Organisation Bill and Melinda Gates Foundation 
Sector Charity/Non Profit
Country United States
Start 04/2018 
End 09/2020
 
Description NC3Rs Research Project Grant
Amount £436,339 (GBP)
Funding ID NC/R001138/1 
Organisation National Centre for the Replacement, Refinement and Reduction of Animals in Research (NC3Rs) 
Sector Charity/Non Profit
Country United Kingdom
Start 12/2017 
End 11/2019
 
Description Newton Fund: The Researcher Links Travel Grants from British Council
Amount £35,400 (GBP)
Organisation British Council 
Sector Charity/Non Profit
Country United Kingdom
Start 08/2016 
End 05/2017
 
Description Newton Fund: UK-China-Philippines-Thailand Swine and Poultry Research Initiative
Amount £841,995 (GBP)
Funding ID BB/R012679/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 01/2018 
End 12/2020
 
Description Novel Mucosal T cells in immunity to swine influenza
Amount £120,000 (GBP)
Funding ID Novel Mucosal T cells in immunity to swine influenza 
Organisation University of Oxford 
Sector Academic/University
Country United Kingdom
Start 10/2016 
End 10/2020
 
Description Passive and active immunity in the porcine influenza model
Amount £120,000 (GBP)
Organisation The Pirbright Institute 
Sector Academic/University
Country United Kingdom
Start 10/2016 
End 01/2021
 
Description Pathfinder BB/R012431/1 Grant holder: Dr Mark Fife. Grant title: chIFITM knockdown/knockout technology as a platform technology for increased vaccine yields in SPF Eggs.
Amount £12,500 (GBP)
Funding ID BB/R012431/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 11/2017 
End 02/2018
 
Description Pirbright Institute Flexible Talent Mobility Account
Amount £180,000 (GBP)
Funding ID BB/S507945/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 11/2018 
End 03/2021
 
Description Recombinant pseudorabies virus as a multivalent vectored vaccine platform for emerging and endemic porcine diseases
Amount £80,000 (GBP)
Organisation The Pirbright Institute 
Sector Academic/University
Country United Kingdom
Start 10/2020 
End 09/2024
 
Description Reducing the Economic and Zoonotic Impact of Avian Influenza (REZIAI): delivering novel vaccines and diagnostics from laboratory to the field.
Amount £400,000 (GBP)
Funding ID BB/S013792/1 
Organisation The Pirbright Institute 
Sector Academic/University
Country United Kingdom
Start 02/2019 
End 01/2021
 
Description Seed Catalyst Award
Amount £25,527 (GBP)
Funding ID ISCF-TFPSA-Pirbright 
Organisation The Pirbright Institute 
Sector Academic/University
Country United Kingdom
Start 11/2018 
End 02/2019
 
Description Taiwan Partnering Award
Amount £40,700 (GBP)
Funding ID BB/S020624/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start  
 
Description The Global Challenges Research Fund
Amount £640,473 (GBP)
Organisation Research Councils UK (RCUK) 
Sector Public
Country United Kingdom
Start 04/2019 
End 03/2024
 
Description The Pirbright Livestock Antibody Hub
Amount $5,500,000 (USD)
Organisation Bill and Melinda Gates Foundation 
Sector Charity/Non Profit
Country United States
Start 11/2019 
End 11/2024
 
Description The Pirbright Livestock Antibody Hub
Amount $5,530,900 (USD)
Funding ID INV-003007 
Organisation Bill and Melinda Gates Foundation 
Sector Charity/Non Profit
Country United States
Start 10/2019 
End 09/2024
 
Description The Pirbright Livestock Antibody Hub
Amount NU$ 5,500,000 (UYU)
Organisation Bill and Melinda Gates Foundation 
Sector Charity/Non Profit
Country United States
Start 09/2019 
End 08/2024
 
Description The genetic and functional analysis of variation in innate immune genes associated with restriction of influenza virus for chicken lines
Amount £422,383 (GBP)
Organisation Aviagen Group 
Sector Private
Country United States
Start 01/2020 
End 03/2021
 
Description The impact of natural genetic variation on ruminant immune responses
Amount £80,000 (GBP)
Organisation University of Southampton 
Sector Academic/University
Country United Kingdom
Start 10/2018 
End 09/2022
 
Description Towards a novel multivalent poultry vaccine: Development of a recombinant infectious bursal disease vectored vaccine encoding conserved B- and T- cell epitopes from infectious bronchitis virus
Amount £5,000 (GBP)
Organisation The Houghton Trust 
Sector Charity/Non Profit
Country United Kingdom
Start 11/2019 
End 11/2020
 
Description UK-China partnership on Global Food Security: Combating avian tumor diseases for sustainable poultry proudction
Amount £382,000 (GBP)
Funding ID BB/R007632/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 04/2018 
End 03/2020
 
Description Understanding the importance of the PI3K pathway in modulating influenza virus replication in chickens and ducks
Amount £100,000 (GBP)
Organisation The Pirbright Institute 
Sector Academic/University
Country United Kingdom
Start 10/2020 
End 04/2024
 
Description [YY-EEID US-UK XXXX] Evaluating how immunosuppression influences influenza A virus transmission and evolution in wild and domestic birds
Amount £606,360 (GBP)
Funding ID BB/T008806/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 06/2019 
End 06/2023
 
Description iCASE studentship: Production and assessment of antiviral prophylactic properties of natural biomolecules against avian influenza and Newcastle disease viruses affecting poultry
Amount £100,000 (GBP)
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 10/2019 
End 09/2023
 
Title A male-specific cell line from a mosquito Anopheles gambiae 
Description A new male-specific cell line from a mosquito Anopheles gambiae has been isolated from sexed neonate larvae. To our knowledge this is the only cell line derived exclusively from male mosquitoes. 
Type Of Material Cell line 
Year Produced 2019 
Provided To Others? No  
Impact The cell line will greatly facilitate studies on components of the sex determination pathway and of dosage compensation machinery in mosquitoes. There are only several cell lines derived from Anopheles gambiae available and these are female-like. Over-expression of genes involved in male sex determination or dosage compensation is detrimental to female cells, hence use of male-derived cells is necessary for such studies. The cell line will become available upon acceptance of a manuscript describing characterization of that cell line. 
 
Title An ex vivo IBDV infection model using a chicken primary B cell culture system 
Description Recently, it has become possible to culture chicken primary B cells in vitro in the presence of a soluble construct of chicken CD40L that was made at The Pirbright Institute. We have demonstrated that these chicken primary B cells can be infected with infectious bursal disease virus (IBDV). This system will be validated and expanded to other B cell tropic viruses in order to replace the use of infected chickens in research, in an NC3Rs funded grant. 
Type Of Material Model of mechanisms or symptoms - in vitro 
Year Produced 2017 
Provided To Others? Yes  
Impact Using this system, we have determined that chicken primary B cells respond to IBDV infection by inducing Type I IFN responses. However, the induction is more pronounced in cells infected with an attenuated strain compared to a very virulent strain. This is consistent with the very virulent strain down-regulating antiviral responses to a greater extent than other strains which may, in part, explain its enhanced virulence. We also observed a reduction in the expression of key genes involved in B cell proliferation and activation following IBDV infection which was only possible by using the primary B cell culture system. 
URL https://www.ncbi.nlm.nih.gov/pubmed/?term=broadbent+dulwich
 
Title CRISPR/Cas9-based gene editing of avian herpesvirus vectors to develop novel recombinant vaccines 
Description Development of recombinant herpesvirus vaccines are usually achieved by classical homologous recombination methods. The advent of new genome editing tools has enabled its application on avian herpesvirus vaccine strains to develop new recombinant vaccines potentially capable of expressing protective antigens from multiple pathogens 
Type Of Material Technology assay or reagent 
Year Produced 2017 
Provided To Others? Yes  
Impact The major impact will be in the generation of novel vaccines that can simultaneously protect against multiple avian diseases 
URL http://www.poultryworld.net/Health/Articles/2016/12/Is-GM-technology-the-future-of-poultry-vaccines-...
 
Title Cattle MHC genotyping 
Description Using the sequence data generated through the targeted pull down of MHC, we developed a full gene and more targeted PCR approach to genotype cattle for the MHC class I region. This has been applied to many hundreds of samples to enable us to select individuals for breeding as well as survey genetic diversity in beef and diary herds. 
Type Of Material Technology assay or reagent 
Year Produced 2018 
Provided To Others? No  
Impact After publication which we anticipate in 2019, we will appy this method to targeted herds and are already attracting industry interest. 
 
Title Comprehensive analysis of genetic variants across immune loci in indigenous breeds. 
Description The interferon-induced transmembrane (IFITM) protein family comprises a class of restriction factors widely characterised in humans for their potent antiviral activity. Their biological activity is well documented in several animal species, but their genetic variation and biological mechanism is less well understood, particularly in avian species. Here we report the complete sequence of the domestic chicken Gallus gallus IFITM locus from a wide variety of chicken breeds to examine the detailed pattern of genetic variation of the locus on chromosome 5, including the flanking genes ATHL1 and B4GALNT4. We have generated chIFITM sequences from commercial breeds (supermarket-derived chicken breasts), ancient bone samples, indigenous chickens from Nigeria (Nsukka) and Ethiopia, European breeds and inbred chicken lines from The Pirbright Institute, totalling of 211 chickens. Through mapping of genetic variants to the latest chIFITM consensus sequence our data reveal that the chIFITM locus does not show structural variation in the locus across the populations analysed, despite spanning diverse breeds from different geographic locations. However, single nucleotide variants (SNVs) in functionally important regions of the proteins within certain groups of chickens were detected, in particular the European breeds and indigenous birds from Ethiopia and Nigeria. In addition, we also found that two out of four SNVs located in the chIFITM1 (Ser36 and Arg77) and chIFITM3 (Val103) proteins were simultaneously under positive selection. Together these data suggest that IFITM genetic variation may contribute to the capacities of different chicken populations to resist viral infection. 
Type Of Material Model of mechanisms or symptoms - non-mammalian in vivo 
Year Produced 2019 
Provided To Others? Yes  
Impact This paper is currently in press in. BMC Genomics. 
 
Title Correlative Light and Electron Microscopy (CLEM) on IBDV-infected cells 
Description Jenny Simpson and Pippa Hawes have developed the technique of Correlative Light and Electron Microscopy (CLEM). This technique has been used to visualise Virus Factories of IBDV. 
Type Of Material Technology assay or reagent 
Year Produced 2019 
Provided To Others? No  
Impact The Virus Factories (VFs) of IBDV were thought to be paracrystalline arrays of virions in the cytoplasm. However, combining our GFP11-tagged IBDV with CLEM technology revealed that the VFs are actually proteinaceous accumulations in the cytoplasm. This has opened up a new avenue of research in terms of the ultrastructure, formation and function of the VFs. 
 
Title Detection of HA antigens of avian influenza viruses 
Description Assay for detection of recombinant HVT expressing HA antigens of avian influenza viruses 
Type Of Material Technology assay or reagent 
Year Produced 2018 
Provided To Others? Yes  
Impact This new method will help to detect recombinant HVT vaccine expressing HA antigen in infected cell. 
 
Title Developed an in vivo infection model to generate highly pathogenic bovine RSV for challenge studies. Developed an accompanying in vitro titration method to calculate yield of virus produced. 
Description Developed an in vivo infection model to generate highly pathogenic bovine RSV for challenge studies. Developed an accompanying in vitro titration method to calculate yield of virus produced. 
Type Of Material Technology assay or reagent 
Provided To Others? No  
Impact Will help use and improve the calf challenge model of bovine RSV for vaccine testing. 
 
Title Development of CLEM techniques for RSV 
Description We have developed the technical expertise to perform correlative light electron microscopy on respiratory syncytial virus infected cells. This allows us to directly compare immunofluorescent images with transmission EM of the same cell. 
Type Of Material Technology assay or reagent 
Year Produced 2019 
Provided To Others? No  
Impact We have recently submitted a paper and grant building on this expertise, as well as forging new collaborations with Diamond light source. 
 
Title Development of assays for quantifying fusion inhibitory antibodies in sera (mFIT assay) 
Description We have developed a micro-fusion inhibition test (mFIT) assay to identify and quantify antibody responses (to vaccines etc.) which are capable of inhibiting viral induced cell-cell fusion. This test has been developed for various negative strand viruses. 
Type Of Material Technology assay or reagent 
Year Produced 2018 
Provided To Others? No  
Impact This assay has allowed us to provide a more detailed examination of neutralising antibody responses to vaccines and when published will be disseminated to the field. 
 
Title Development of high-throughput assay for examining paramyxovirus fusion 
Description We have developed and optimised high-throughput assays (96 well plate format) to examine paramyxovirus fusion, using an adapted Bi-FC approach. We are using this to identify inhibitors of paramyxovirus, e.g. measles and RSV, fusion and attachment proteins. This has been integrated with next-generation platforms such as the Incucyte real-time imaging system available at The Pirbright Institute. 
Type Of Material Technology assay or reagent 
Year Produced 2018 
Provided To Others? No  
Impact We are using this assay to examine the innate immune restriction of paramyxovirus replication. 
 
Title Development of tetramers to study immune responses in pigs 
Description The pig is a natural and important host of influenza viruses and is physiologically more comparable to humans than other animal models in terms of size, respiratory tract biology and volume. It is also an important vector in the birds to human infection cycle. A major drawback of the current pig model is the inability to analyze antigen-specific CD8+ T-cell responses, which are critical to respiratory immunity. We addressed this knowledge gap using an established in-bred pig model with a high degree of genetic identity between individuals, including the MHC (Swine Leukocyte Antigen (SLA)) locus. We developed a toolset that included long-term in vitro pig T-cell culture and cloning and identification of novel immunodominant influenza-derived T-cell epitopes. We also generated structures of the two SLA class I molecules found in these animals presenting the immunodominant epitopes. These structures allowed definition of the primary anchor points for epitopes in the SLA binding groove and established SLA binding motifs that were used to successfully predict other influenza-derived peptide sequences capable of stimulating T-cells. 
Type Of Material Technology assay or reagent 
Year Produced 2017 
Provided To Others? No  
Impact Peptide-SLA tetramers were constructed and used to track influenza-specific T-cells ex vivo in blood, the lungs and draining lymph nodes. Aerosol immunization with attenuated single cycle influenza viruses (S-FLU) induced large numbers of CD8+ T-cells specific for conserved NP peptides in the respiratory tract. Collectively, these data substantially increase the utility of pigs as an effective model for studying protective local cellular immunity against respiratory pathogens. 
 
Title Established a method to immortalize bovine antigen presenting cells 
Description Established protocols to prepare lentiviruses expressing the proto-oncogenes Bmi1 and hTERT. Established protocols to transduce and generate immortal bovine antigen presenting cells derived from peripheral blood monocytes. 
Type Of Material Cell line 
Provided To Others? No  
Impact This is the first successful attempt at generating bovine immortal monocytic cell lines. This method can also be applied to immortalize B cells. This will result in reducing the number of animals used in experimentation and considerably reduce costs. 
 
Title Established human T cell lines expressing bovine WC1 genes for the analysis of WC1 signal transduction pathways. 
Description Established human Jurkat T cells expressing bovine the bovine gammadelta WC1 co-receptor 
Type Of Material Cell line 
Provided To Others? No  
Impact This cell line will help determine the function of the WC1 co-receptor in gammadelta T cell activation and function. 
 
Title Generated a MHC-defined bovine monophagocytic cell line. 
Description Using the method described above, successfully generated one MHC-defined bovine monophagocytic cell line (A18) which expresses and single classical MHC gene (HD6). 
Type Of Material Cell line 
Provided To Others? No  
Impact Will help reduce the number of animals used in experimentation. Will help resolve antigen presentation pathways in bovine cells and using bovine pathogens. Will help understand T cell activation pathways using MHC matched and mis-matched cells. Will help investigate bovine NK cell activation. 
 
Title Generated monophagocytic reporter cell lines to study the NF-kB signaling pathway 
Description Generated human monophagocytic cells expressing alkaline phosphatase reporter gene under a NF-kB promoter sequence. 
Type Of Material Cell line 
Provided To Others? No  
Impact This cell line is helping determine the role of RSV genes in immune modulation of monophagocytic cells in response to RSV infection. 
 
Title IBDV Reverse Genetics System 
Description We have developed a reverse genetics system for IBDV. 
Type Of Material Technology assay or reagent 
Provided To Others? No  
Impact We have used the reverse genetics system to generate recombinant IBDVs and chimeric recombinant IBDVs that contain genes from classical and very virulent strains of IBDV in the background of a cell culture adapted attenuated strain. This will enable us to study the effect of individual virus genes on pathogenicity. 
 
Title Method to evenly load flowcells with multiple samples 
Description Pirbright has an Illumina NextSeq sequencing machine installed in containment. Given the large amount of sequencing reads produced by each run, multiple samples are usually multiplexed on the same flowcell. However, determining the correct concentrations needed to obtain the same amount of reads for each sample is a hard problem, and usually requires a number of expensive and material-consuming kits to be run prior to sequencing. We have developed a method that allows samples to be evenly multiplexed based only on Bioanalyzer data (we routinely run Bioanalyser on the samples anyway as a form of quality control). The method works well, and reliably across a wide range of sample concentrations and sequenced organisms. 
Type Of Material Technology assay or reagent 
Year Produced 2018 
Provided To Others? No  
Impact The method greatly increased the quality of our output, and drastically simplified our workflow by eliminating the external quantification steps that were previously required. 
 
Title Optimisation of chicken kidney cell culture preparation 
Description We have optimised the method of extracting kidneys and preparing chicken kidney (CK) cell cultures from chickens. CK cells are used for in vitro assays to study avian viruses, in particular infectious bronchitis virus. 
Type Of Material Cell line 
Year Produced 2017 
Provided To Others? No  
Impact This has increased the yield of viable cells and the quality of the cell cultures. We can now obtain the number of cells we require using fewer chicken kidneys, therefore requiring fewer chickens to be culled. This is an important development for the 3Rs. We hope to publish this method for others' information. 
 
Title Optimisation of preparation of TOCs from adult chickens 
Description Tracheal organ cultures (TOCs) are prepared from chicken embryos and used to assess growth of avian viruses that are not able to be propagated in cell culture. We have optimised the method of TOCs preparation from 3-week old chickens and are using these to assess viral genetic stability and phenotype in organ cultures that are a similar environment to the site of replication in whole chickens. 
Type Of Material Cell line 
Year Produced 2018 
Provided To Others? No  
Impact We hope that this technique will reduce the number of chickens required for in vivo experiments, in line with the 3Rs, and plan to publish the method in due course. 
 
Title Stable chicken cell lines overexpressing chicken IFITM1,2 and 3 
Description In collaboration with Mark Fife, we have produced chicken stable cell lines overexpressing chicken IFITM1,2, 3 and a mutant chicken IFITM3 lacking palmitoylation sites. 
Type Of Material Technology assay or reagent 
Year Produced 2017 
Provided To Others? No  
Impact These cell lines will be used to evaluate the effect of the IFITM proteins on the replication of different viruses, including IBDV. 
 
Title UniMMap- a pipeline for mapping RNAseq data over repetitive immune complexes. 
Description To exploit the abundance of available short read sequencing data we have developed a pipeline that uses mappability to accurately measure transcription over repetitive gene complexes. This methods uses known haplotypes to examine regions of uniqueness, and then the RNAseq data from the individual to train the method to be species or individual specific. This is particularly important over gene complexes that contain genes involved in the immune system, that are often highly similar in sequence but can have profoundly different functions. 
Type Of Material Technology assay or reagent 
Year Produced 2018 
Provided To Others? No  
Impact the immediate impact will be adding fine resolution data to livestock gene expression atlas projects lead by the FAANG consortium. Further impact will be arising as this method is published and we apply it to numerous existing datasets the are publically avaialable as well as generated at Pirbright and by our collaborators. 
 
Title chimeric IBDVs 
Description Andrew Broadbent has engineered chimeric IBDVs expressing individual genes from a very virulent strain in the backbone of an attenuated strain. 
Type Of Material Technology assay or reagent 
Year Produced 2018 
Provided To Others? No  
Impact These chimeric strains can be used to define viral determinants of virulence. 
 
Title in vitro model of IBDV antigenic drift in chicken B cells 
Description IBDV vaccines do not induce sterilising immunity, meaning vaccinated birds can become infected with field strains. As for other viruses, it is thought that vaccine-induced immune selection pressure drives the evolution of antigenic drift variants that accumulate amino acid changes in the hypervariable region of the capsid, which may lead to vaccine failures. However, antigenic drift has not been studied in any detail for IBDV, and there is therefore a paucity of information regarding how plastic the capsid is, how quickly mutations arise and become fixed in the virus population, or whether some are more dominant than others. One way of studying this is to vaccinate chickens with sub-protective doses and then challenge them with field strains of IBDV and then sequence the resulting viruses that emerge. To replace the use of birds in these experiments, we have developed an in vitro model of antigenic drift by serially passaging a field strain, F5270, in the immortalised chicken B cell line, DT40, in the presence of sub-neutralising concentrations of vaccine-induced antibodies to better characterise changes in the capsid. 
Type Of Material Technology assay or reagent 
Year Produced 2019 
Provided To Others? No  
Impact In our model, amino acid mutations arose in the hypervariable region of the IBDV capsid at the same sites previously observed in field studies, validating our model. Furthermore, we demonstrated that mutations arose early, by only 5 passages in chicken B cells, which subsequently became fixed by 10 passages. We are now doing next generation sequencing to better characterise the plasticity of the capsid. 
 
Title method for identification of lung Tissue resident memory cells in pigs 
Description Recent overwhelming evidence indicates the importance of local tissue-resident memory T cells (TRM) in protective immunity. Most work on TRM has been performed in mice and the TRM defined as inaccessible to intravenously administered anti-T cell antibody. However there are very few data on TRM in large animals. For the first time we have defined TRM in the pig influenza model. 
Type Of Material Technology assay or reagent 
Year Produced 2018 
Provided To Others? No  
Impact The identification of TRM and their robust protective capacity in site-specific infection has provided a new paradigm by which to assess T cell-mediated responses and an important new target for vaccine design. Since swine are an economically important species, are used as a large animal model for human infection and play a key role in the emergence of novel and potentially zoonotic influenza viruses, the identification of TRM in pigs will allow us to study their role in immunity to swine influenza.and how best to induce them by immunisation. 
 
Title replication competent recombinant split-GFP infectious bursal disease virus 
Description We have generated a replication competent recombinant split-GFP infectious bursal disease virus (IBDV) where a small GFP11 molecule is tagged to the virus polymerase (VP1) to make IBDV-VP1-GFP11. Infected cells that express the GFP1-10 molecule fluoresce green when the GFP11 tag complements the GFP1-10. The IBDV-VP1-GFP11 colocalises with VP3 and dsRNA and therefore is likely to be present at the sites of virus replication. 
Type Of Material Technology assay or reagent 
Year Produced 2017 
Provided To Others? No  
Impact This tool can be used to study IBDV replication complex development in infected cells. 
 
Title tetracysteine-tagged IBDV 
Description Andrew Broadbent has engineered a tetracysteine (TC)-tagged IBDV that can be used in live cell imaging and co-infection studies with the split-GFP IBDV. 
Type Of Material Technology assay or reagent 
Year Produced 2018 
Provided To Others? No  
Impact This tool, combined with the split-GFP IBDV, will enable us to study co-infection, super-infection exclusion, and the intracellular requirements for viral reassortment. 
 
Title Analysis of paramyxovirus replication using Incucyte real-time imager 
Description We have optimised a data analysis pipeline for the Incucyte real-time imager to allow us to track viral replication in a high-throughput fashion (up to 6 x 96-well plates). This is based on simultaneous analysis of phase, as well as green and red fluorescence. 
Type Of Material Data analysis technique 
Year Produced 2018 
Provided To Others? No  
Impact Only preliminary data to date. 
 
Title E-MTAB-5947- Transcription profiling by array of differential gene expression in chicken primary B cells infected ex vivo with attenuated and very virulent strains of infectious bursal disease virus (IBDV) 
Description Microarray data submitted to ArrayExpress from chicken primary B cells infected with a very virulent and attenuated strain of IBDV. 
Type Of Material Database/Collection of data 
Year Produced 2017 
Provided To Others? Yes  
Impact This is the first ever microarray data of chicken primary B cells infected with IBDV and will be of use to other researchers in the area of B cell biology or IBDV virology 
URL https://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-5947/
 
Title Full length cattle MHC genes 
Description Using our method for full length MHC gene amplification, the largest reference set ever produced from common haplotypes has been sequenced and deposited in the publicly available data IPD-MHC with associated research tools. 
Type Of Material Database/Collection of data 
Year Produced 2020 
Provided To Others? No  
Impact None yet, but it will allow far greater resolution when analysing MHC polymorphism and evolution in cattle populations. 
URL https://www.ebi.ac.uk/ipd/mhc/group/BoLA/
 
Description Aerosol delivery of vaccines and therapeutics 
Organisation Aerogen
Country Ireland 
Sector Private 
PI Contribution Developed the pig influenza model which is a natural host pathogen system
Collaborator Contribution Provided expertise and equipment for aerosol delivery
Impact Successfully delivered vaccines by aerosol
Start Year 2015
 
Description Andrew Davidson - Proteome of viral replication organelles 
Organisation University of Bristol
Department Avon Longitudinal Study of Parents and Children (ALSPAC)
Country United Kingdom 
Sector Academic/University 
PI Contribution Projects are being initiated to study the cellular proteins associated with coronavirus replication organelle membranes and a funding application has been submitted to BBSRC.
Collaborator Contribution Andrew has provided assistance with experimental planning and drafting of a grant application. He has also provided protocols to allow preliminary data to be generated. Once protocols have been established, Andrew will facilitate mass spectrometry data generation and will analyse the resulting data.
Impact No outcomes to date, projects being initiated and funding applications in progress.
Start Year 2018
 
Description Annika Posautz; University of Veterinary Medicine Vienna. Morbillivirus host-diversity analysis. 
Organisation University of Veterinary Medicine Vienna
Country Austria 
Sector Academic/University 
PI Contribution Sharing of research data regarding morbillivirus host-range and technical support to the project.
Collaborator Contribution Provision of tissue samples from potential morbillivirus hosts, e.g. carnivores.
Impact No outcomes as yet.
Start Year 2018
 
Description BBSRC - Oxford University iCASE Studentship (October 2019- September 2023). Production and assessment of antiviral prophylactic properties of natural biomolecules against avian influenza and Newcastle disease viruses affecting poultry production 
Organisation University of Oxford
Country United Kingdom 
Sector Academic/University 
PI Contribution This collaboration between The Pirbright Institute (UK), Oxford University (UK) and industrial partners in Italy. This project will conceived and developed bu MI. The PhD student (Holly Everest) in my group is investigating the antiviral prophylactic properties of natural biomolecules against avian influenza and Newcastle disease viruses affecting poultry production.
Collaborator Contribution The partners in Italy will produce and purify natural antiviral molecules effective against avian influenza and other poultry viruses.
Impact This project started in January 2020.
Start Year 2020
 
Description BBSRC-UC Davis workshop, 16-18 October 
Organisation University of California, Davis
Department UC Davis College of Biological Sciences
Country United States 
Sector Academic/University 
PI Contribution Presented on "Reproducibility and relevance in vector infection studies". Followed up with discussion of potential publication and funding opportunities.
Collaborator Contribution Various presentations on vectors and vector-borne diseases including, facilities, challenges, opportunities.
Impact Discussion of potential joint publications and grant applications; no tangible impacts yet.
Start Year 2017
 
Description BBSRC-UC Davis workshop, 16-18 October 
Organisation University of Liverpool
Country United Kingdom 
Sector Academic/University 
PI Contribution Presented on "Reproducibility and relevance in vector infection studies". Followed up with discussion of potential publication and funding opportunities.
Collaborator Contribution Various presentations on vectors and vector-borne diseases including, facilities, challenges, opportunities.
Impact Discussion of potential joint publications and grant applications; no tangible impacts yet.
Start Year 2017
 
Description Bioimaging collaboration with Dr. Daniel Clare, Dr. James Gilchrist, Dr. Pippa Hawes - cryo-electron tomography Diamond Light Source 
Organisation Diamond Light Source
Country United Kingdom 
Sector Private 
PI Contribution Development of preliminary data on inclusion body structure to support higher resolution approaches.
Collaborator Contribution Discussion of project and experimental design, technological approaches and limitations.
Impact No outputs to date. Multi-disciplinary team of virologists and structural biologists
Start Year 2020
 
Description Biophysics collaboration with Prof. Stephen McKnight, UTSW-Dallas 
Organisation University of Texas Southwestern Medical Center
Country United States 
Sector Academic/University 
PI Contribution Development of preliminary data on the biophysics and biochemistry of inclusion bodies
Collaborator Contribution Supportive comments on grant applications and experimental design advice.
Impact No outputs to date
Start Year 2020
 
Description Bridget Penman 
Organisation University of Warwick
Department School of Life Sciences
Country United Kingdom 
Sector Academic/University 
PI Contribution We are providing genetic data and diversity measures of MHC and associated receptors to inform the modelling efforts to elucidate which selection pressures are the main drivers for the types of diversity we see in extant species.
Collaborator Contribution They are experts in mathmatical modelling and will provide data to help explain genetic diversity in cattle.
Impact Not yet
Start Year 2017
 
Description Challenge of transgenic chickens with influenza viruses 
Organisation Imperial College London
Department Section of Virology
Country United Kingdom 
Sector Academic/University 
PI Contribution We have challenged transgenic chickens with recombinant avian influenza viruses to determine outcome; infectivity, transmission, pathogenicity, dissemination within host. We also processed the samples generated from these animal experiments at The Pirbright Institute.
Collaborator Contribution Imperial College (Wendy Barclay and Mike Skinner) generated recombinant avian influenza viruses for challenge.
Impact This collaboration has resulted in the successful award of a joint grant with imperial college aiming to carry out challenge experiments on novel transgenic chickens to facilitate control of avian influenza virus in the poultry population. BB/S007911/1 - Investigating the role of ANP32A in the replication of avian Influenza virus
Start Year 2018
 
Description Charles Sande - KEMRI - Kenya: RSV collaboration 
Organisation Kenyan Institute for Medical Research (KEMRI)
Country Kenya 
Sector Public 
PI Contribution Development of research proposal for PhD studentship on the innate immune response during RSV infection. This student will be hosted by my lab for 18 months from 2020 onwards.
Collaborator Contribution Project design. Application for PhD funding and provision of technical support.
Impact The studentship was awarded to Dr. Sande in Kenya and the student has been recruited. They will commence their studies in Spring 2019 and come to the UK in early 2020.
Start Year 2018
 
Description Chicken Microbiome - Robert La Ragione - University of Surrey 
Organisation University of Surrey
Department School of Veterinary Medicine
Country United Kingdom 
Sector Academic/University 
PI Contribution Providing sequencing data of gut microbiome from chickens infected with avian influenza viruses.
Collaborator Contribution Sharing expertise in the analysis of microbiome data
Impact Joint manuscripts and funding opportunities are being explored
Start Year 2019
 
Description Collaboration on replication of mumps virus in cells 
Organisation University of Copenhagen
Country Denmark 
Sector Academic/University 
PI Contribution Intellectual input so far, training research assistant to prepare samples, imaging samples in transmission electron microscopes
Collaborator Contribution Preliminary confocal microscopy to optimize sample conditions. Preparation of samples for EM.
Impact No outcomes yet
Start Year 2018
 
Description Collaboration with University of Copenhagen CFIM on cell biology of virus-host interactions 
Organisation University of Copenhagen
Country Denmark 
Sector Academic/University 
PI Contribution Intellectual input into virus projects which come in to the microscopy core facility at the University. Limited practical work and training given to researchers there. Includes lecturing/organisation of MSc and PhD courses at Copenhagen. PH awarded Visiting Professorship.
Collaborator Contribution Research projects are presented to the core facility, I advise on the best route for achieving the data required and those researchers complete preliminary data following my advice. Collaborators are then able to produce the best possible samples for further advanced microscopy as necessary.
Impact No tangible outcomes yet as we are in early stages. This is multi-disciplinary as projects can come from several University departments (medical, pathology, cell biology, veterinary, food), and other Universities in Denmark (DTU). University awarded Visiting Professorship
Start Year 2018
 
Description Commonwealth Scholarship. Molecular determinants impacting avian influenza H9N2 virus evolution, replication fitness and virulence 
Organisation Royal Veterinary College (RVC)
Country United Kingdom 
Sector Academic/University 
PI Contribution I develop a research project proposal in which we are investigating how evolutionary changes in the H9N2 avian influenza virus modulate virus fitness and virulence in chickens. This PhD studentship project was funded by Commonwealth scholarship commission for 3 years.
Collaborator Contribution The collaborating Partner Dr Nicola Lewis at Royal Veterinary College is a co-supervisor of the PhD student Thusitha Karunarathna. she provide necessary guidance to achieve prescribed objectives of this project.
Impact The project is at its initial stage.
Start Year 2019
 
Description Components of the Medea gene drive system in a mosquito Aedes aegypti 
Organisation National Health Research Institutes (NHRI) Taiwan
Department Institute of Molecular and Genomic Medicine
Country Taiwan, Province of China 
Sector Charity/Non Profit 
PI Contribution Developed joined research project and lead successful application for funding
Collaborator Contribution Various lines of research, including construction of plasmids and development and characterisation of transgenic mosquito strains; and group discussions about results, conclusions and research progress.
Impact No impact yet.
Start Year 2018
 
Description Components of the Medea gene drive system in a mosquito Aedes aegypti 
Organisation National Institute of Genetics
Department NIG-FLY, Mishima (Drosophila RNAi resources)
Country Japan 
Sector Academic/University 
PI Contribution Developed joined research project and lead successful application for funding
Collaborator Contribution Various lines of research, including construction of plasmids and development and characterisation of transgenic mosquito strains; and group discussions about results, conclusions and research progress.
Impact No impact yet.
Start Year 2018
 
Description DTU 
Organisation Technical University of Denmark
Country Denmark 
Sector Academic/University 
PI Contribution Joint PhD studentship
Collaborator Contribution Joint PhD studentship
Impact Joint PhD studentship in progress
Start Year 2016
 
Description David Hughes - University of St. Andrews. RSV preparation. 
Organisation St. Andrews University
Country United States 
Sector Academic/University 
PI Contribution Sharing of preliminary research data on innate immune restriction of RSV.
Collaborator Contribution Provision of cell-lines (Hep2-Npro) to support generation of high titre stocks of RSV. Also associated protocols.
Impact No outcomes as yet, beyond preliminary data.
Start Year 2018
 
Description David Matthews - Proteomics informed by transcriptomics 
Organisation University of Bristol
Department Proteomics Facility
Country United Kingdom 
Sector Academic/University 
PI Contribution Projects are being initiated to study the cellular proteins associated with coronavirus replication organelle membranes and a funding application has been submitted to BBSRC.
Collaborator Contribution David has assisted in project design and drafting of a funding application to BBSRC. We are in the process of generating transcriptomics data, which David will process to provide an expressed protein database for peptide identification following mass spectrometry.
Impact No outcomes to date, a funding application has been submitted to BBSRC.
Start Year 2018
 
Description Derek Bickhart-USDA 
Organisation U.S. Department of Agriculture USDA
Department Beltsville Agricultural Research Center
Country United States 
Sector Academic/University 
PI Contribution Our group has provided the genomic framework of the cattle NK cell regions and the biological input.
Collaborator Contribution The ARS group has used our data as a framework to map and predict cattle SNPs for variable immune traits using their whole genome data
Impact Research application based on preliminary data to a BBSRC-NIFA call. This collaboration is multi-disciplinary combining molecular biology and computational biology
Start Year 2014
 
Description Development of tetramers in the Babraham pig model 
Organisation Cardiff University
Country United Kingdom 
Sector Academic/University 
PI Contribution Developed the aerosol delivery method of vaccine and provided material (spleen, BAL and blood) from inbred animals (Babraham pigs) following immunisation with the candidate universal vaccine, S-FLU.
Collaborator Contribution Developed porcine influenza specific class I tetramers and provided the epitope map of Babrahams S-Flu responses to NP, M1, M2, PB1 and PB2 that could be presented by SLA-1 or SLA-2 Grew pig T cell clones for the first time in 30 years.
Impact Multidisciplinary - immunology, virology, protein chemistry. Paper not yet published
Start Year 2014
 
Description Distributed Bio - Gates foundation funding - universal influenza vaccine 
Organisation Distributed Bio.
Country United States 
Sector Private 
PI Contribution Pirbright has provided expert advice to the design of adjuvant and challenge studies in appropriate animal models for human influenza vaccine trials. Pirbright is facilitating these studies in line with UK home office requirements and good scientific practice.
Collaborator Contribution Distributed Bio have developed a novel vaccine that is hoped to be a candidate for a human universal influenza virus vaccine.
Impact Funding from Bill and Melinda Gates foundation as part of the Grand challenges - Universal vaccine for influenza for the animal studies involving Pirbright and Distributed Bio
Start Year 2019
 
Description Dr Alex Schock, APHA - Detemining the prevalence and sequences of IBDV in vaccinated chicken flocks in the UK 
Organisation Animal and Plant Health Agency
Country United Kingdom 
Sector Public 
PI Contribution We will determine whether samples are positive for IBDV. If they are positive, we will amplify the hypervariable region of the capsid by PCR and send for Sanger Sequencing
Collaborator Contribution APHA will obtain samples from Veterinary Practices and perform histology.
Impact we have been successful in obtaining funding (Pirbright Internal Seed Award, £10,000)
Start Year 2019
 
Description Dr Carol Cardona, University of Minnesota- avian influenza virus challenge of IBDV or mock-infected chickens 
Organisation University of Minnesota
Country United States 
Sector Academic/University 
PI Contribution We will challenge birds that have been exposed to IBDV, or mock exposed, with highly pathogenic avian influenza (HPAI) strains to determine how IBDV-mediated immunosuppression impacts upon HPAI infection in chickens.
Collaborator Contribution Dr Cardona's group will challenge birds that have been exposed to IBDV, or mock exposed, with low pathogenic avian influenza (LPAI) strains to determine how IBDV-mediated immunosuppression impacts upon LPAI infection in chickens.
Impact we have been successful in obtaining funding (Grant Ref: BB/T008806/1)
Start Year 2019
 
Description Dr David Welchman, APHA -Detemining the prevalence and sequences of IBDV in vaccinated chicken flocks in the UK 
Organisation Animal and Plant Health Agency
Country United Kingdom 
Sector Public 
PI Contribution We will determine whether samples are positive for IBDV. If they are positive, we will amplify the hypervariable region of the capsid by PCR and send for Sanger Sequencing
Collaborator Contribution APHA will obtain samples from Veterinary Practices and perform histology.
Impact we have been successful in obtaining funding (Pirbright Internal Seed Award, £10,000)
Start Year 2019
 
Description Dr Finn Grey, The Roslin Institute- Identifying genes essential for the replicaiton of IBDV and IBV in vitro 
Organisation University of Edinburgh
Department The Roslin Institute
Country United Kingdom 
Sector Academic/University 
PI Contribution We will conduct a CRISPR screen and identify genes that are essential for IBDV replication
Collaborator Contribution Finn Grey's group will aid in the CRISPR screen
Impact we have been successful in obtaining a PhD studentship
Start Year 2019
 
Description Dr Helena Maier, The Pirbright Institute - Identifying genes essential for the replicaiton of IBDV and IBV in vitro 
Organisation The Pirbright Institute
Country United Kingdom 
Sector Academic/University 
PI Contribution We will conduct a CRISPR screen and identify genes that are essential for IBDV replication
Collaborator Contribution Helena Maier's group will aid in the CRISPR screen and identify genes that are essential for IBV replication
Impact We have been successful in obtaining a PhD studentship
Start Year 2019
 
Description Dr Holly Shelton, The Pirbright Institute- avian influenza virus challenge of IBDV or mock-infected chickens 
Organisation The Pirbright Institute
Country United Kingdom 
Sector Academic/University 
PI Contribution We will expose birds to IBDV, compared to mock controls
Collaborator Contribution Holly will train my group in conducting studies with avian influenza viruses and, together, we will challenge birds with highly pathogenic avian influenza (HPAI) strains to determine how IBDV-mediated immunosuppression impacts upon HPAI infection in chickens.
Impact we have been successful in obtaining funding (Grant Ref: BB/T008806/1)
Start Year 2019
 
Description Dr Liam Morrison 
Organisation University of Edinburgh
Department The Roslin Institute
Country United Kingdom 
Sector Academic/University 
PI Contribution Analysis is single cattle B cell antibody sequences
Collaborator Contribution Single cattle B cell antibody sequences
Impact None as yet
Start Year 2019
 
Description Dr Lyndsay Fry 
Organisation Washington State University
Department Washington State University Spokane
Country United States 
Sector Academic/University 
PI Contribution Single B cell sorting and antibody sequencing, developing a new methods for Theileria sp.
Collaborator Contribution Providing funding and the samples from immunised animals. We have also engineered an antibody to aid in the sorting of cattle B cells
Impact None as yet
Start Year 2019
 
Description Dr Michael Skinner, Imperial College London 
Organisation Imperial College London
Department Department of Primary Care and Public Health
Country United Kingdom 
Sector Academic/University 
PI Contribution We aim to compare the host transcriptional response of primary chicken B cells infected with different strains of IBDV of varying virulence. We have the specialist reagents and expertise in house that are required to culture primary chicken B cells, and we have access to different strains of IBDV.
Collaborator Contribution Dr Michael Skinner and his team at Imperial College London have the expertise and facilities to generate and analyse RNA-Seq data in order to determine host transcriptional responses. This is required in order to complete the aim of the project.
Impact Further funding: PhD studentship (£102,800) - Dr Andrew Broadbent at The Pirbright Institute and Dr Michael Skinner at Imperial College London are joint supervisors for a PhD student working on this project. Further Funding: Houghton Trust (£8,000)- Dr Andrew Broadbent was awarded a Houghton Trust Small Research Grant to contribute to this project. Further funding: NC3Rs (Grant Ref: NC/R001138/1)
Start Year 2014
 
Description Dr Rachel Edgar, Imperial College London- defining the circadian clock in chicken cells and how this impacts upon viral replication 
Organisation Imperial College London
Country United Kingdom 
Sector Academic/University 
PI Contribution we will supply sequences of chicken clock genes and chicken cells and challenge synchronised chicken cells with IBDV and perform in vivo experiments
Collaborator Contribution Dr Edagr's group will synchronise chicken cells and characterise the oscillatory expression of chicken clock genes.
Impact we have been successful in obtaining funding (Houghton Trust, £4,500)
Start Year 2019
 
Description Efficacy of a candidate universal influenza vaccine, S-FLU. 
Organisation University of Oxford
Department Radcliffe Department of Medicine
Country United Kingdom 
Sector Academic/University 
PI Contribution Tested the immunogenicity and efficacy of a candidate universal influenza vaccine, S-FLU, in pigs. For the first time we demonstrated that S-FLU when administered by aerosol can reduce viral load in nasal swabs and lung in pigs after influenza virus challenge. We have shown that the most efficient way to administer this vaccine is by aerosol.
Collaborator Contribution Professor Alain Townsend has developed the S-FLU vaccine.
Impact The most efficient way to induce immune response in the lung is after aerosol delivery of LAIV vaccines in pigs. The most efficient way to induce cross-protective immunity is by aerosol delivery of S-FLU to the lungs of pigs
Start Year 2014
 
Description Engineering mosquitoes for reduced vector competence for chikungunya virus 
Organisation University of Malaya
Department Department of Medical Microbiology
Country Malaysia 
Sector Academic/University 
PI Contribution Developed joint research programme, led successful application for funding, coordinate programme.
Collaborator Contribution Various specific strands of research, and group discussions about results, conclusions, research progress and future activities.
Impact reported under funding
Start Year 2016
 
Description Engineering mosquitoes for reduced vector competence for chikungunya virus 
Organisation University of Melbourne
Country Australia 
Sector Academic/University 
PI Contribution Developed joint research programme, led successful application for funding, coordinate programme.
Collaborator Contribution Various specific strands of research, and group discussions about results, conclusions, research progress and future activities.
Impact reported under funding
Start Year 2016
 
Description Engineering mosquitoes for reduced vector competence for chikungunya virus 
Organisation University of Tartu
Department Institute of Technology
Country Estonia 
Sector Academic/University 
PI Contribution Developed joint research programme, led successful application for funding, coordinate programme.
Collaborator Contribution Various specific strands of research, and group discussions about results, conclusions, research progress and future activities.
Impact reported under funding
Start Year 2016
 
Description Evaluation of bovine herpesvirus-4 as a vaccine vector for pigs 
Organisation University of Parma
Country Italy 
Sector Academic/University 
PI Contribution Expertise in porcine vaccinology/immunology
Collaborator Contribution Expertise in the generation of recombinant bovine herpesvirus-4 (BoHV-4) vectors expressing heterologous antigens
Impact We demonstrated that immunisation of pigs with recombinant BoHV-4 vectors expressing Nipah virus glycoproteins are highly immunogenic in pigs. Most promising and as hypothesised, were the potent CD4 and CD8 T cell responses which were an order of greater than those induced by other viral vaccine vectors. These data have led to the external funding of projects by two companies to evaluate BoHV-4 vectors in the context of other porcine diseases.
Start Year 2018
 
Description Evaluation of immune checkpoint inhibitors as molecular adjuvants for improved PRRSV vaccines 
Organisation Leidos
Country United States 
Sector Private 
PI Contribution Expertise in PRRSV immunology
Collaborator Contribution Patented immune checkpoint inhibitors
Impact No yet
Start Year 2019
 
Description Evaluation of mRNA vectored vaccines in pigs 
Organisation University of Pennsylvania
Country United States 
Sector Academic/University 
PI Contribution Expertise in porcine immunology/vaccinology
Collaborator Contribution Expertise in mRNA vaccines
Impact We evaluated in pigs the immunogenicity an mRNA vector encoding a Nipah virus glycoprotein. This vaccine formulation induced high virus neutralising antibody titres (comparable with protein subunit in adjuvant) and T cell responses (comparable with viral vectors). We also utilised mRNA immunised pigs to isolate Nipah virus neutralising monoclonal antibodies.
Start Year 2018
 
Description Evaluation of solid dose vaccines for livestock 
Organisation Enesi Pharma
Country United Kingdom 
Sector Private 
PI Contribution Expertise in PRRSV and vaccine evaluation
Collaborator Contribution Expertise in vaccine formulation
Impact Multidisciplinary collaboration between formulation scientist and viral immunologists.
Start Year 2019
 
Description Factor H and influenza virus 
Organisation Brunel University London
Department Brunel Arts
Country United Kingdom 
Sector Academic/University 
PI Contribution Our research team are hosting a PhD student from Brunel University to facilitate the investigation of any interaction between influenza virus and complement factor H. We are providing reagents, expertise in handling influenza virus and advice on the scientific hypothesis of the research programme.
Collaborator Contribution Brunel university initiated the project and provided reagents including antibodies and methods for purification human co-factor H protein. They also provide funding for this PhD studentship.
Impact No outcomes as yet
Start Year 2018
 
Description Gene editing chickens with Roslin 
Organisation University of Edinburgh
Department Roslin Institute Transgenic Chicken Facility
Country United Kingdom 
Sector Academic/University 
PI Contribution We have provided infectious pathogen challenge and infectious pathogen sample processing facilities in this collaboration. We infected the transgenic chickens with avian influenza virus and processed the samples following challenge.
Collaborator Contribution Helen Sang and Mike McGrew at The Roslin Institute (University of Edinburgh) contributed by producing and providing transgenic chickens (Split GFP) for challenge. Expertise in genotype screening the transgenic birds was shared with us.
Impact We have now successfully received a BBSRC responsive mode grant to carry out more transgenic bird challenges with influenza viruses. BB/S007911/1 -Investigating the role of ANP32A in the replication of avian Influenza virus.
Start Year 2018
 
Description Generation of immortalised bovine antigen presenting cells 
Organisation University of Surrey
Country United Kingdom 
Sector Academic/University 
PI Contribution Using a lentivirus vector expressing proto-oncogenic genes, we generated MHC-defined immortal bovine antigen presenting cells that can be used in antigen presentation studies or virus-host interaction studies in the future.
Collaborator Contribution The University of Surrey provided several lenvirus vectors and preparation for immortalization studies.
Impact A publication in progress titled "Generation of immortal bovine antigen presenting cells" and the generation of a library of MHC-defined bovine cell lines.
Start Year 2017
 
Description Horizon Discovery Ltd Cambridge Research Park, United Kingdom 
Organisation Horizon Discovery Group plc
Country United Kingdom 
Sector Private 
PI Contribution Verify IFITM knock-out cell by qPCR and immuno-blotting. Verification of further knock-out cell lines by DNA sequencing, immuno-blotting and qPCR Cell infection with Avian Influenza Viruses Assess the viral titre of cells infected with Influenza Viruses in K/O and wildtype cells. Quantitative analysis of viral infection in IFITM K/O cells Demonstrate increased AIV viral titres at lab scale and estimation of what this may equate to with respect to vaccine dose yields. Analysis of IFITM K/O cell lines permissivity to a range of additional animal viruses (Relevant to the LVIF). Milestone 7: Demonstrate permissivity increased and viral titres for a range of LVIF relevant viruses in the edited cell lines.
Collaborator Contribution Develop a CRISPR/cas9 gene editing system to specifically target the chIFITM locus in avian cell culture
Impact Grant funding
Start Year 2017
 
Description ILRI Research Collaboration (Kenya, March 2017) 
Organisation International Livestock Research Institute (ILRI)
Country Kenya 
Sector Charity/Non Profit 
PI Contribution Exchange of ideas and samples for indigenous breeds of livestock
Collaborator Contribution Contributed samples for analysis.
Impact Analysis of indigenous breed genomic sequences for Immune function genes.
Start Year 2017
 
Description Identification of conserved B-cell epitopes of highly pathogenic coronaviruses for broadspectrum immunotherapy and vaccine design. 
Organisation Imperial College London
Country United Kingdom 
Sector Academic/University 
PI Contribution We have performed experiments to generate preliminary data for a collaborative grant application, contributed to the writing of the grant proposal and responded to reviewers comments.
Collaborator Contribution My collaborators have performed experiments to generate preliminary data for a collaborative grant application, contributed to the writing of the grant proposal and responded to reviewers comments.
Impact Submission of proposal entitled "Identification of conserved B-cell epitopes of highly pathogenic coronaviruses for broadspectrum immunotherapy and vaccine design" to the "One Health Approaches to Accelerate Vaccine Development" call that forms part of the UK government's commitment to Official Development Assistance (ODA) in October 2017.
Start Year 2017
 
Description Identification of conserved B-cell epitopes of highly pathogenic coronaviruses for broadspectrum immunotherapy and vaccine design. 
Organisation University of Kent
Country United Kingdom 
Sector Academic/University 
PI Contribution We have performed experiments to generate preliminary data for a collaborative grant application, contributed to the writing of the grant proposal and responded to reviewers comments.
Collaborator Contribution My collaborators have performed experiments to generate preliminary data for a collaborative grant application, contributed to the writing of the grant proposal and responded to reviewers comments.
Impact Submission of proposal entitled "Identification of conserved B-cell epitopes of highly pathogenic coronaviruses for broadspectrum immunotherapy and vaccine design" to the "One Health Approaches to Accelerate Vaccine Development" call that forms part of the UK government's commitment to Official Development Assistance (ODA) in October 2017.
Start Year 2017
 
Description Identification of human orthologues of bovine Workshop Cluster 1 genes 
Organisation University of Oxford
Department Nuffield Department of Medicine
Country United Kingdom 
Sector Academic/University 
PI Contribution The Workshop Cluster 1 (WC1) gene family encodes a group of proteins thought to be involved in immune recognition. These genes are exclusively expressed on T cells expressing the gamma and delta T cell receptor and are well characterized in the bovine system. We have began investigating the presence and expression of WC1 on human cells using co-immunoprecipitation and mass spectrometry analysis of putatitve human WC1 genes.
Collaborator Contribution The Univ. of Oxford has developed a pipeline to identify protein fragments using mass spectrometry and bioinformatics from putative proteins from which little or no information is currently known. This pipeline has been applied to identify putative human WC1 proteins obtained from co-immunoprecipitation/mass spectrometry studies.
Impact The identification of one human WC1 gene/protein. A publication in progress titled "Identification of the human orthologue of a Workshop Cluster 1 gene expressed on gammadelta T cells"
Start Year 2017
 
Description Identification of sows with antibodies broadly neutralising PRRSV 
Organisation University of Lleida
Country Spain 
Sector Academic/University 
PI Contribution Expertise in PRRSV immunology
Collaborator Contribution Access to large numbers of sows routinely vaccinated and exposed to PRRSV
Impact Not yet
Start Year 2019
 
Description Imperial 
Organisation Imperial College London
Country United Kingdom 
Sector Academic/University 
PI Contribution Joint supervision of PhD student
Collaborator Contribution Joint supervision of PhD student
Impact One successful completed PhD student. Papers published or in preparation. Another PhD student in progress.
Start Year 2010
 
Description Influenza and co-factor H protein 
Organisation Leibniz Association
Department Leibniz Institute for Natural Product Research and Infection Biology
Country Germany 
Sector Academic/University 
PI Contribution We are screening influenza virus interaction with co-factor H proteins to determine the interaction interface.
Collaborator Contribution Christine Skerka has provided fragment factor H peptides to facilitate our mapping studies of the interaction surface with influenza viruses.
Impact No outcomes as yet
Start Year 2019
 
Description Investigating antigenic determinants inducing stronger and broader cross-protective immunity among H5 avian influenza viruses 
Organisation Royal Veterinary College (RVC)
Department Veterinary Basic Sciences
Country United Kingdom 
Sector Academic/University 
PI Contribution The overarching aims of the proposed research are to improve controls against H5 subtype of avian influenza viruses infecting poultry by investigating underlying mechanisms that define how vaccines renders effectiveness and develop novel approaches enhancing the effectiveness of H5 avian influenza vaccines. We recently generated a panel of monoclonal antibodies (mAbs) that recognise a variety of different clades of H5 viruses. Propagation of AIV in eggs or cultured cells together with mAbs drives virus to escape from mAbs neutralisation activity. These classical virus neutralization assays (VN) followed by HA gene sequence analysis allowed us to defined the role of amino acid substitutions that are associated with evasion of antibody neutralization through emergence of antigenic variants and failure of vaccine efficacy.
Collaborator Contribution provided intellectual input for achieving prescribed objectives of this work.
Impact Talk entitled "Molecular determinants for antigenicity and vaccine efficacy of avian influenza viruses" was presented at Newton Agham Researcher Links Workshop " Novel Vaccines and Diagnostic Technologies against Emerging and re-emerging Veterinary Pathogens" at Rizal Park Hotel, Manila, Philippines, 4-7 February 2019.
Start Year 2019
 
Description Investigating antigenic determinants inducing stronger and broader cross-protective immunity among H5 avian influenza viruses. 
Organisation Royal Veterinary College (RVC)
Country United Kingdom 
Sector Academic/University 
PI Contribution We developed research programme to characterise diversity of antigenic epitopes induce protective immunity in chickens against H5 subtypes of avian influenza viruses.
Collaborator Contribution Collaborating partner Dr Nicola Lewis at Royal Veterinary College provide advise to on cartography programme to visualize the antigenic similarity or difference among different strains of influenza viruses.
Impact The work under this collaborative determined the antigenic differences among different clades of H5 avian influenza viruses circulating in birds. This project is multi-disciplinary. our laboratory mainly work on virology and immunology part of the project and we seek help on computer modeling part from the collaborating partner Dr Nicola Lewis.
Start Year 2019
 
Description Investigating the role of the SH protein of RSV in the duration of immunity 
Organisation Imperial College London
Department Department of Medicine
Country United Kingdom 
Sector Academic/University 
PI Contribution We provided initial data supporting the hypothesis that the small hydrophobic protein of bovine respiratory syncytial virus regulates the host's immune response by modulating the transcription factors NF-kB and STAT1 in bovine cells
Collaborator Contribution Based on our results, Imperial has began investigating the role of the small hydrophobic protein of human respiratory syncytial virus on human cells.
Impact Publication in progress titled "Regulation of the B cell activating factor BAFF by the small hydrophobic protein of respiratory syncytial virus".
Start Year 2017
 
Description Invitation to participate in the Vaccine Innovation Forum 2017 at Shanghai 26-27 April 2017 
Organisation Chinese Academy of Agricultural Sciences
Department Shanghai Veterinary Research Institute
Country China 
Sector Public 
PI Contribution Prof. Nair was invited to participate in the Vaccine Innovation Forum 2017 at Shanghai 26-27 April 2017 to present a talk on 'Novel Vaccine Development for Avian Virus Diseases'. He also participated in the round table discussions on the challenges and opportunities in the vaccines and vaccination sector
Collaborator Contribution Vaccine Innovation Forum will help in expanding the future collaborations and activities of the UK-China Centre of Excellence for Research on Avian Diseases (http://www.uk-china-cerad.org/).
Impact organised joint meeting in China and the UK to discuss on challenges and opportunities
Start Year 2017
 
Description JN - Preparation of capped mesoporpus silica nanoparticles 
Organisation Polytechnic University of Valencia
Country Spain 
Sector Academic/University 
PI Contribution We were involved with testing the nanoparticles once they had been made. We also contributed purified antibody and shipped it to our collaborators for the generation of one type of nanoparticle, which we subsequently tested. We were involved with the intellectual design of experiments
Collaborator Contribution Our partners generated the nanoparticles and shipped them to us for testing. Our partners were also involved with the intellectual design of experiments.
Impact Experimental work performed to investigate the use of mesoporous silica nanoparticles to detect FMDV proteases in clinical samples
Start Year 2016
 
Description JS - Production of monoclonal antibodies that recognise FMDV VP4 from mouse spleens 
Organisation University of Edinburgh
Department The Roslin Institute
Country United Kingdom 
Sector Academic/University 
PI Contribution VLPs displaying the N-terminal 15 amino acids of FMDV were designed and produced at The Pirbright Institute and then mice were immunised with them. The spleens of the immunised mice were harvested and splenocytes stored. The response to the VP4 sequence was checked by ELISA and the mouse with the best response was selected to send the splenocytes for monoclonal production.
Collaborator Contribution The collaborators took the splenocytes obtained from the mouse experiments and carried out the fusions. They then screened the supernatants for positive wells that detected the VP4 sequence.
Impact Positive wells have been identified indicating some monoclonals that are specific for VP4 have been generated. These will be further screened for ability to recognise virus and to neutralise infection.
Start Year 2017
 
Description Janet Daly & Stephen Dunham- PI3K pathway and influenza 
Organisation University of Nottingham
Department School of Veterinary Medicine and Science Nottingham
Country United Kingdom 
Sector Academic/University 
PI Contribution Preliminary data was produced by Pirbright using reverse genetics of avian influenza viruses to secure a 50:50 funded PhD studentship that will commence in October 2020 entitled "Understanding the importance of the PI3K pathway in modulating influenza virus replication in chickens and ducks".
Collaborator Contribution Preliminary data was produced by University of Nottingham using PI3K p85 subunit pulldowns to secure a 50:50 funded PhD studentship that will commence in October 2020 entitled "Understanding the importance of the PI3K pathway in modulating influenza virus replication in chickens and ducks".
Impact Funding for a PhD studentship entitled "Understanding the importance of the PI3K pathway in modulating influenza virus replication in chickens and ducks".
Start Year 2019
 
Description Leeds FBS 
Organisation University of Leeds
Department Leeds Institute of Molecular Medicine
Country United Kingdom 
Sector Academic/University 
PI Contribution Research
Collaborator Contribution Research and student supervision
Impact Research
Start Year 2009
 
Description Linking sex determination and dosage compensation in a mosquito Anopheles gambiae 
Organisation University of Zurich
Department Institute of Molecular Life Sciences
Country Switzerland 
Sector Academic/University 
PI Contribution Developed a new research program.
Collaborator Contribution Provided intellectual input, contributed to drafting a joined grant proposal.
Impact No output yet.
Start Year 2019
 
Description Martin Ludlow - University of Veterinary Medicine, Hanover. Morbillivirus collaboration. 
Organisation University of Veterinary Medicine Hanover
Country Germany 
Sector Academic/University 
PI Contribution Provision of technical information and advice about the host-range of morbilliviruses.
Collaborator Contribution Provision of reagents to support our research project, e.g. plasmid clones of viral attachment proteins and receptors.
Impact No outcomes as yet, only preliminary data.
Start Year 2018
 
Description Miguel Ángel Muñoz Alía - Mayo clinic, USA. Measles population diversity. 
Organisation Mayo Clinic
Country United States 
Sector Charity/Non Profit 
PI Contribution Technical support and sharing of research data on the host-range of morbilliviruses.
Collaborator Contribution Provision of plasmid clones representing the broad genetic diversity of measles attachment and fusion proteins. Technical support.
Impact No outcomes as yet.
Start Year 2018
 
Description Miles Carroll PHE collaboration on pseudotyping 
Organisation Public Health England
Country United Kingdom 
Sector Public 
PI Contribution Provision of reagents to perform pseudotyping of Ebola G protein
Collaborator Contribution Discussion of preliminary data
Impact None to date, publications in preparation.
Start Year 2019
 
Description Mosquito Interest Group 
Organisation Imperial College London
Country United Kingdom 
Sector Academic/University 
PI Contribution Organising and attending meetings of students, RAs, postdocs and PIs on mosquito biology and related techniques.
Collaborator Contribution Organising and attending meetings of students, RAs, postdocs and PIs on mosquito biology and related techniques.
Impact Exchange of practical techniques, for instance in tracking oviposition of individual mosquitoes. Brainstorming for projects and papers, resulting in a GCRF VBD network proposal (which passed the first review but was not funded in the second) and an informal collaboration (which was intended for submission to UK-Thai call in 2017 but Thai partner was not eligible). Exchanges of colony strains of insects planned this month.
Start Year 2016
 
Description Mucosally Associated Invariant T cells (MAIT) in pig influenza model 
Organisation University of Oxford
Country United Kingdom 
Sector Academic/University 
PI Contribution Identified MAIT cells in pig Influenza model. Provide samples from infected and immunised protected animals
Collaborator Contribution Provide expertise in characterising MAIT: development of tetramer; Zell scanner technology allowing high resolution studies of cell phenotype and functions
Impact Joint PhD studentship with Paul Klenerman, University of Oxford
Start Year 2016
 
Description Next-generation PRRSV vaccines 
Organisation Huvepharma
Country Bulgaria 
Sector Private 
PI Contribution Expertise in PRRSV virology and immunology
Collaborator Contribution Funding
Impact Too early, project ongoing.
Start Year 2019
 
Description Nicolas Locker - Investigation of translation regulation by infectious bronchitis virus 
Organisation University of Surrey
Country United Kingdom 
Sector Academic/University 
PI Contribution My team provide experience in molecular virology and cellular interactions of infectious bronchitis virus including previous experience of IBV accessory proteins regulating cellular translation. A PhD student works jointly between our labs.
Collaborator Contribution Dr Locker's team provide expertise in viral regulation of cellular translation and stress granule signalling. Dr Locker is also providing protocols, advice and training for techniques including ribopuromycylation and ribosome profiling. A PhD studentworks jointly between our labs.
Impact PhD student funding (studentship commenced Oct 2017) - Control of host cell translation by infectious bronchitis virus
Start Year 2016
 
Description Outreach Network for Gene Drive Research 
Organisation Outreach Network for Gene Drive Research
Sector Charity/Non Profit 
PI Contribution Input to policy discussions
Collaborator Contribution Network is engaged in briefing national and international entities, e.g. EU/EC, European Parliament, IUCN, Convention on Biological Diversity, on issues related to gene drive. Outreach also relates to general public (directly and via media) as well as policymakers, regulators and practitioners. The Pirbright Institute is now a member of the Network
Impact Briefing and media comment regarding gene drives and gene drive research to multiple agencies e.g. EU/EC, European Parliament, IUCN, Convention on Biological Diversity, on issues related to gene drive.
Start Year 2019
 
Description Oxford - Strubi 
Organisation University of Oxford
Country United Kingdom 
Sector Academic/University 
PI Contribution Collaboration on virus structural biology and joint PhD studentship
Collaborator Contribution Collaboration on virus structural biology and joint PhD studentship
Impact Collaboration on virus structural biology has produced a number of novel virus structures and new understanding of virus entry and packaging
Start Year 2008
 
Description Oxford University Collaborations 
Organisation University of Oxford
Department Big Data Institute
Country United Kingdom 
Sector Academic/University 
PI Contribution Scientific discussion, collaboration and manuscript preparation
Collaborator Contribution Scientific discussion, collaboration and manuscript preparation
Impact Manuscript in preparation
Start Year 2017
 
Description Parham Lab at Stanford 
Organisation Stanford University
Department School of Medicine
Country United States 
Sector Academic/University 
PI Contribution Our group designed the experiments and generated a majority of the data. We also led the analysis of the data and writing of the manuscripts
Collaborator Contribution The Parham lab is the leading group worldwide in the evolutionary study of NK cell receptors and MHC class I. This group generated part of the data and helped in the analysis, interpretation and manuscript preparation
Impact Definition of the Cattle Killer Cell Igndash like Receptor Gene Family: Comparison with Aurochs and Human Counterparts Nicholas D. Sanderson, Paul J. Norman, Lisbeth A. Guethlein, Shirley Ellis, Christina Williams, Matthew Breen, Steven D. E. Park, David A. Magee, Farbod Babrzadeh, Andrew Warry, Mick Watson, Daniel G. Bradley, David E. MacHugh, Peter Parham, and John A. Hammond. Journal of Immunology, Dec 15, 2014; 193 (12) This collaboration is not multi-didciplinary
Start Year 2009
 
Description Partnering with National Taiwan University 
Organisation National Taiwan University
Country Taiwan, Province of China 
Sector Academic/University 
PI Contribution We have prepared and submitted an application for a BBSRC-Taiwan Partnering Award to study "Assembly of Recombinant Infectious Bronchitis Virus and Determination of Antigenic Sites that Confer Hemagglutination Activity".
Collaborator Contribution The partners conceived the project and got in contact about submitting an application for funding together.
Impact An application has been made for a BBSRC-Taiwan Partnering Award in November 2018.
Start Year 2018
 
Description Paul Digard & Reidun Twarock - Packaging signals 
Organisation University of Edinburgh
Department The Roslin Institute
Country United Kingdom 
Sector Academic/University 
PI Contribution Supply of reverse genetic avian influenza viruses for the successful award of a PhD studentship based at The Roslin Institute entitled "Identification of interaction sites between the genomic segments of influenza virus as putative novel anti-viral targets".
Collaborator Contribution University of York is providing mathematical modelling algorithms of viral packaging. Paul Digard- Roslin Institute is providing influenza virology expertise and hosting the student.
Impact Studentship commenced in October 2019
Start Year 2019
 
Description Paul Digard & Reidun Twarock - Packaging signals 
Organisation University of York
Country United Kingdom 
Sector Academic/University 
PI Contribution Supply of reverse genetic avian influenza viruses for the successful award of a PhD studentship based at The Roslin Institute entitled "Identification of interaction sites between the genomic segments of influenza virus as putative novel anti-viral targets".
Collaborator Contribution University of York is providing mathematical modelling algorithms of viral packaging. Paul Digard- Roslin Institute is providing influenza virology expertise and hosting the student.
Impact Studentship commenced in October 2019
Start Year 2019
 
Description Paul Digard - The Roslin Insititute 
Organisation University of Edinburgh
Department The Roslin Institute
Country United Kingdom 
Sector Academic/University 
PI Contribution We generated constructs that express PB1-F2 from a variety of avian influenza strains in eukaryotic expression plasmids. We generated Chimera PB1-F2 expression plasmids where the C-terminus of the PB1-F2 are switched between strains. Using these plasmids we have determined the localization and antagonist activity toward the IFN beta signaling pathway and NF-kb signaling pathway in human and chicken cells. We have therefore provided reagents and intellectual input to the collaboration.
Collaborator Contribution Our collaborators have used these plasmids in there well defined stability assays to determine sequence specific effects on this characteristic. Intellectual input and expertise in a particular assay were made by these collaborators.
Impact We have successfully published some of this work; "The cellular localization of avian influenza virus PB1-F2 protein alters the magnitude of IFN2 promoter and NF?B-dependent promoter antagonism in chicken cells." doi: 10.1099/jgv.0.001220. We have an ongoing joint PhD studentship in this area. This collaboration remains current and active.
Start Year 2017
 
Description Paul Verkade - Electron tomography and CLEM 
Organisation University of Bristol
Department Avon Longitudinal Study of Parents and Children (ALSPAC)
Country United Kingdom 
Sector Academic/University 
PI Contribution Nicole Doyle has performed electron tomography to analyse the three dimensional structure of membrane rearrangements induced by the expression of IBV non-structural proteins 4 or 3 and 4 in the absence of other viral components. Sample set up was performed at Pirbright and analysis was subsequently performed at University of Bristol followed by additional analysis at Pirbright.
Collaborator Contribution Paul has assisted with electron tomography data generation providing access to electron microscopes in Bristol as well as training for Nicole Doyle in performing experiments and analysing and modelling data. He is also advising Nicole while establishing correlative light electron microscopy at Pirbright.
Impact doi: 10.3390/v10090477
Start Year 2018
 
Description Pirbright-Diamond collaboration 
Organisation Diamond Light Source
Country United Kingdom 
Sector Private 
PI Contribution 50% of my time is now based at Diamond, I am considered in-house staff and can apply to use the instrumentation. I feed projects to eBIC for collaboration using advanced electron microscopy, centered around cell biology of host-pathogen interactions using advanced electron microscopy, plus potential use of Xray microscopy (on application).
Collaborator Contribution Instrument time on cryo-FIB-SEM and screening TEM (two days per month). Diamond have loaned us a plunge freezing device.
Impact No outputs yet
Start Year 2019
 
Description Professor Benedikt Kaufer 
Organisation Free University of Berlin
Country Germany 
Sector Academic/University 
PI Contribution Providing knowledge and methodologies.
Collaborator Contribution Providing reagents and methodologies to perform experiments.
Impact Two publications from this collaboration: Gurung A, Kamble N, Kaufer BB, Pathan A, Shahriar Behboudi. Association of Marek's Disease induced immunosuppression with activation of a novel regulatory T cells in chickens, PLoS Pathogens, 2017, 13 (12), e1006745. Boodhoo N, Kamble N, Kaufer BB, Shahriar Behboudi. Replication of Marek's disease virus is dependent on de novo synthesis of fatty acid and Prostaglandin E2. J Virol. 2019 Apr 10
Start Year 2019
 
Description Professor Helen Sang,The Roslin Institute - GFP1-10 expressing transgenic chickens 
Organisation University of Edinburgh
Department The Roslin Institute
Country United Kingdom 
Sector Academic/University 
PI Contribution We have developed IBDV tagged to GFP11
Collaborator Contribution Helen Sang's group at the Roslin Institute have developed transcgenic chicken lines that constitutively express GFP1-10 in every cell.
Impact we have been successful in obtaining funding (Grant Ref: BB/S014594/1)
Start Year 2019
 
Description Professor Shayan Sharif, University of Guelph, Canada 
Organisation University of Guelph
Department Department of Pathobiology
Country Canada 
Sector Academic/University 
PI Contribution Collaboration between my team and Professor Sharif lead to new finding in Avian immunology, specifically in understanding the effects of nutrients on antigen presenting cells and T cells of chicken. We took the lead in studying the effects of some nutrients (Vitamin D) on the function of chicken T cells, performed most of the experiments in our laboratory. Meanwhile, we participated in studying the effects of Vitamin D on antigen presenting cells of chicken by performing some key experiments because we had access to some specific reagents (monoclonal antibodies) recognizing molecules expressed on chicken antigen presenting cells.
Collaborator Contribution Professor Sharif team took the lead in studying the effects of Vitamin D on antigen presenting cells of chicken, and contributed to experimental plan for studying the effects on chicken T cells.
Impact Two publications in 2015 and 2016: 1. Nitish Boodhoo, Shayan Sharif, Shahriar Behboudi. 1a,25(OH)2 Vitamin D3 Modulates Avian T Lymphocyte Functions without Inducing CTL Unresponsiveness. PLoS One. 2016 Feb 24;11(2):e0150134 2. Bahram Shojadoost, Shahriar Behboudi, Villanueva AI, Jennifer Brisbin, Ali Ashkar, Shayan Sharif. Vitamin D3 modulates the function of chicken macrophages. Res. Vet. Sci. 2015 June, 100: 45-51
Start Year 2019
 
Description Pseudoparticles of influenza for interaction studies with co-factor H 
Organisation University of Kent
Department Medway School of Pharmacy
Country United Kingdom 
Sector Hospitals 
PI Contribution We have screened the interaction between influenza pseudotype particles that only contain the surface HA protein from influenza viruses for interaction with human co-factor H protein. We have screen pseudotype particles of multiple influenza subtypes.
Collaborator Contribution Nigel Temperton of the viral Pseudotype Unit at the School of Pharmacy provided influenza virus pseudotype particles of multiple subtypes to allow us to screen for interaction with co-factor H protein.
Impact No outcomes yet
Start Year 2018
 
Description Pseudotyping virus collaboration with Dr. Tess Lambe, University of Oxford 
Organisation University of Oxford
Country United Kingdom 
Sector Academic/University 
PI Contribution Development of assays and reagents to support successful pseudotyping of various viruses for quantitative entry assays
Collaborator Contribution Provision of reagents and plasmid clones
Impact No outputs yet,
Start Year 2019
 
Description Rapid acquisition of mammalian characteristics by avian influenza virus in single host infections. 
Organisation Imperial College London
Country United Kingdom 
Sector Academic/University 
PI Contribution Provided reagents and day to day technical help for undertaking this project and supervising postdoctoral researcher working on this project. Reagents include influenza reverse genetic plasmids and H9N2 and H7N9-specific antibodies.
Collaborator Contribution this project will aim to understand AIV genetics that facilitate the rapid acquisition of mammalian adaptation characteristics in a single host infection. The partners with our collaborations determined adaptive genetic changes in the H9N2 avian influenza virus following infection in mice (mammalian hosts). Different strains of avian influenza Viruses generated by reverse gentic techniques were inoculated into mice and lungs sampled daily. RNA recovered from lung homogenates were deep sequenced and mutations arising were characterised for adaptation. Relative viral fitness and the rapidity of the accumulation of mutations was measured and compared amongst the viral strains.
Impact The results of the our experimental studies showed the avian-origin viruses rapidly acquired mutations that increase virus fitness in mammalian species. Therefore, these viruses pose zoonotic and pandemic threat to public health.
Start Year 2018
 
Description Rapid acquisition of mammalian characteristics by avian influenza virus in single host infections. 
Organisation The Pirbright Institute
Country United Kingdom 
Sector Academic/University 
PI Contribution Provided reagents and day to day technical help for undertaking this project and supervising postdoctoral researcher working on this project. Reagents include influenza reverse genetic plasmids and H9N2 and H7N9-specific antibodies.
Collaborator Contribution this project will aim to understand AIV genetics that facilitate the rapid acquisition of mammalian adaptation characteristics in a single host infection. The partners with our collaborations determined adaptive genetic changes in the H9N2 avian influenza virus following infection in mice (mammalian hosts). Different strains of avian influenza Viruses generated by reverse gentic techniques were inoculated into mice and lungs sampled daily. RNA recovered from lung homogenates were deep sequenced and mutations arising were characterised for adaptation. Relative viral fitness and the rapidity of the accumulation of mutations was measured and compared amongst the viral strains.
Impact The results of the our experimental studies showed the avian-origin viruses rapidly acquired mutations that increase virus fitness in mammalian species. Therefore, these viruses pose zoonotic and pandemic threat to public health.
Start Year 2018
 
Description Reannotation of the Anopheles gambiae genome 
Organisation Biomathematics and Statistics Scotland (BioSS)
Country United Kingdom 
Sector Public 
PI Contribution Generated and provided RNA-seq data from the developmental transcriptome of the mosquito Anopheles gambiae Manually browsed the transcriptomic data in the genome context and provided coordinates for genome regions with not annotated genes or genes requiring annotation corrections. Generated and provided FAIRE-seq data from embryo samples of Anopheles gambiae to identify regulatory elements controlling early embryo development.
Collaborator Contribution Conducting reannotation of the Anopheles gambiae genome based on RNA-seq data from the developmental transcriptome. Mapping the FAIRE-seq data to the genome and linking the mapped data the RNA-seq data.
Impact No outputs yet
Start Year 2017
 
Description Reannotation of the Anopheles gambiae genome 
Organisation Centre for Genomic Regulation (CRG)
Country Spain 
Sector Academic/University 
PI Contribution Generated and provided RNA-seq data from the developmental transcriptome of the mosquito Anopheles gambiae Manually browsed the transcriptomic data in the genome context and provided coordinates for genome regions with not annotated genes or genes requiring annotation corrections. Generated and provided FAIRE-seq data from embryo samples of Anopheles gambiae to identify regulatory elements controlling early embryo development.
Collaborator Contribution Conducting reannotation of the Anopheles gambiae genome based on RNA-seq data from the developmental transcriptome. Mapping the FAIRE-seq data to the genome and linking the mapped data the RNA-seq data.
Impact No outputs yet
Start Year 2017
 
Description Reannotation of the Anopheles gambiae genome 
Organisation Imperial College London
Country United Kingdom 
Sector Academic/University 
PI Contribution Generated and provided RNA-seq data from the developmental transcriptome of the mosquito Anopheles gambiae Manually browsed the transcriptomic data in the genome context and provided coordinates for genome regions with not annotated genes or genes requiring annotation corrections. Generated and provided FAIRE-seq data from embryo samples of Anopheles gambiae to identify regulatory elements controlling early embryo development.
Collaborator Contribution Conducting reannotation of the Anopheles gambiae genome based on RNA-seq data from the developmental transcriptome. Mapping the FAIRE-seq data to the genome and linking the mapped data the RNA-seq data.
Impact No outputs yet
Start Year 2017
 
Description Research Collaboration with Nigeria (23rd - 28th January 2017) 
Organisation University of Nigeria
Country Nigeria 
Sector Academic/University 
PI Contribution Analysis of rare breed indigenous chicken in Nigeria.
Collaborator Contribution Helped with sampling and background information of the breed.
Impact Analysis is ongoing.
Start Year 2017
 
Description Stephen Graham collaboration on structural biology with University of Cambridge 
Organisation University of Cambridge
Country United Kingdom 
Sector Academic/University 
PI Contribution Provision of data and sharing of experimental ideas regarding viral host range and techniques for simulating virus evolution
Collaborator Contribution Structural analysis of mutants to viral glycoproteins
Impact Journal of Virology paper on potential PPRV emergence in human populations (Abdullah et al, 2018), other papers in preparation.
Start Year 2018
 
Description Steve Goodbourn - St Georges - chicken innate molecule interactions. 
Organisation Ulster University
Department Biomedical Sciences Research Centre
Country United Kingdom 
Sector Academic/University 
PI Contribution We have collaborated by producing yeast- 2-hybrid plasmids containing the influenza PB1-F2 gene from various avian influenza strains. The PhD student on this studentship then visited Professor Goodbourn's laboratory to carry out the yeast-2-hybrid screen for interaction innate chicken molecules.
Collaborator Contribution Professor Goodbourn's laboratory provided yeast-2-hybrid plasmids containing various innate chicken molecules to be screened for interaction with avian influenza virus PB1-F2 protein. They also offered help and guidance carrying out the interaction technique to understand if PB1-F2 directly associated with chicken innate components. They also provided reporter plasmids and guidance on innate reporter assays in chicken cells.
Impact Successful publication of work. doi: 10.1099/jgv.0.001220. The cellular localization of avian influenza virus PB1-F2 protein alters the magnitude of IFN2 promoter and NF?B-dependent promoter antagonism in chicken cells.
Start Year 2016
 
Description Supply of Arthropods to External Partners 
Organisation Animal and Plant Health Agency
Country United Kingdom 
Sector Public 
PI Contribution Culicoides nubeculosus, C. sonorensis (biting midge) and Culex pipiens (mosquito) provided as pupae for insecticide, repellent and vector competence trials as a resource. UK supplies conducted under National Capability Grant. Approximately 30 000 insects shipped during reporting period for academic users. All insect production took place at Pirbright and training in maintenance was provided to a worker from FLI (Germany).
Collaborator Contribution Daughter line of C. sonorensis was established at FLI (improving security around sustaining the lines). Training and advice on insect maintenance and handling was provided by Pirbright to LSHTM, Liverpool and Reading. Studies then carried out included investigation of bacteria in Culicoides (University of Liverpool), modeling of mosquito populations (Reading) and development of novel insecticides and repellent formulations (LSHTM). These specialist expertise were driven by the host organizations for the studies.
Impact Products are still in development.
Start Year 2017
 
Description Supply of Arthropods to External Partners 
Organisation Bern University of Applied Sciences
Country Switzerland 
Sector Academic/University 
PI Contribution Culicoides nubeculosus, C. sonorensis (biting midge) and Culex pipiens (mosquito) provided as pupae for insecticide, repellent and vector competence trials as a resource. UK supplies conducted under National Capability Grant. Approximately 30 000 insects shipped during reporting period for academic users. All insect production took place at Pirbright and training in maintenance was provided to a worker from FLI (Germany).
Collaborator Contribution Daughter line of C. sonorensis was established at FLI (improving security around sustaining the lines). Training and advice on insect maintenance and handling was provided by Pirbright to LSHTM, Liverpool and Reading. Studies then carried out included investigation of bacteria in Culicoides (University of Liverpool), modeling of mosquito populations (Reading) and development of novel insecticides and repellent formulations (LSHTM). These specialist expertise were driven by the host organizations for the studies.
Impact Products are still in development.
Start Year 2017
 
Description Supply of Arthropods to External Partners 
Organisation ETH Zurich
Country Switzerland 
Sector Academic/University 
PI Contribution Culicoides nubeculosus, C. sonorensis (biting midge) and Culex pipiens (mosquito) provided as pupae for insecticide, repellent and vector competence trials as a resource. UK supplies conducted under National Capability Grant. Approximately 30 000 insects shipped during reporting period for academic users. All insect production took place at Pirbright and training in maintenance was provided to a worker from FLI (Germany).
Collaborator Contribution Daughter line of C. sonorensis was established at FLI (improving security around sustaining the lines). Training and advice on insect maintenance and handling was provided by Pirbright to LSHTM, Liverpool and Reading. Studies then carried out included investigation of bacteria in Culicoides (University of Liverpool), modeling of mosquito populations (Reading) and development of novel insecticides and repellent formulations (LSHTM). These specialist expertise were driven by the host organizations for the studies.
Impact Products are still in development.
Start Year 2017
 
Description Supply of Arthropods to External Partners 
Organisation Friedrich Loeffler Institute
Country Germany 
Sector Academic/University 
PI Contribution Culicoides nubeculosus, C. sonorensis (biting midge) and Culex pipiens (mosquito) provided as pupae for insecticide, repellent and vector competence trials as a resource. UK supplies conducted under National Capability Grant. Approximately 30 000 insects shipped during reporting period for academic users. All insect production took place at Pirbright and training in maintenance was provided to a worker from FLI (Germany).
Collaborator Contribution Daughter line of C. sonorensis was established at FLI (improving security around sustaining the lines). Training and advice on insect maintenance and handling was provided by Pirbright to LSHTM, Liverpool and Reading. Studies then carried out included investigation of bacteria in Culicoides (University of Liverpool), modeling of mosquito populations (Reading) and development of novel insecticides and repellent formulations (LSHTM). These specialist expertise were driven by the host organizations for the studies.
Impact Products are still in development.
Start Year 2017
 
Description Supply of Arthropods to External Partners 
Organisation Lancaster University
Country United Kingdom 
Sector Academic/University 
PI Contribution Culicoides nubeculosus, C. sonorensis (biting midge) and Culex pipiens (mosquito) provided as pupae for insecticide, repellent and vector competence trials as a resource. UK supplies conducted under National Capability Grant. Approximately 30 000 insects shipped during reporting period for academic users. All insect production took place at Pirbright and training in maintenance was provided to a worker from FLI (Germany).
Collaborator Contribution Daughter line of C. sonorensis was established at FLI (improving security around sustaining the lines). Training and advice on insect maintenance and handling was provided by Pirbright to LSHTM, Liverpool and Reading. Studies then carried out included investigation of bacteria in Culicoides (University of Liverpool), modeling of mosquito populations (Reading) and development of novel insecticides and repellent formulations (LSHTM). These specialist expertise were driven by the host organizations for the studies.
Impact Products are still in development.
Start Year 2017
 
Description Supply of Arthropods to External Partners 
Organisation London School of Hygiene and Tropical Medicine (LSHTM)
Country United Kingdom 
Sector Academic/University 
PI Contribution Culicoides nubeculosus, C. sonorensis (biting midge) and Culex pipiens (mosquito) provided as pupae for insecticide, repellent and vector competence trials as a resource. UK supplies conducted under National Capability Grant. Approximately 30 000 insects shipped during reporting period for academic users. All insect production took place at Pirbright and training in maintenance was provided to a worker from FLI (Germany).
Collaborator Contribution Daughter line of C. sonorensis was established at FLI (improving security around sustaining the lines). Training and advice on insect maintenance and handling was provided by Pirbright to LSHTM, Liverpool and Reading. Studies then carried out included investigation of bacteria in Culicoides (University of Liverpool), modeling of mosquito populations (Reading) and development of novel insecticides and repellent formulations (LSHTM). These specialist expertise were driven by the host organizations for the studies.
Impact Products are still in development.
Start Year 2017
 
Description Supply of Arthropods to External Partners 
Organisation Queen's University Belfast
Country United Kingdom 
Sector Academic/University 
PI Contribution Culicoides nubeculosus, C. sonorensis (biting midge) and Culex pipiens (mosquito) provided as pupae for insecticide, repellent and vector competence trials as a resource. UK supplies conducted under National Capability Grant. Approximately 30 000 insects shipped during reporting period for academic users. All insect production took place at Pirbright and training in maintenance was provided to a worker from FLI (Germany).
Collaborator Contribution Daughter line of C. sonorensis was established at FLI (improving security around sustaining the lines). Training and advice on insect maintenance and handling was provided by Pirbright to LSHTM, Liverpool and Reading. Studies then carried out included investigation of bacteria in Culicoides (University of Liverpool), modeling of mosquito populations (Reading) and development of novel insecticides and repellent formulations (LSHTM). These specialist expertise were driven by the host organizations for the studies.
Impact Products are still in development.
Start Year 2017
 
Description Supply of Arthropods to External Partners 
Organisation Texas A&M University-Central Texas
Country United States 
Sector Academic/University 
PI Contribution Culicoides nubeculosus, C. sonorensis (biting midge) and Culex pipiens (mosquito) provided as pupae for insecticide, repellent and vector competence trials as a resource. UK supplies conducted under National Capability Grant. Approximately 30 000 insects shipped during reporting period for academic users. All insect production took place at Pirbright and training in maintenance was provided to a worker from FLI (Germany).
Collaborator Contribution Daughter line of C. sonorensis was established at FLI (improving security around sustaining the lines). Training and advice on insect maintenance and handling was provided by Pirbright to LSHTM, Liverpool and Reading. Studies then carried out included investigation of bacteria in Culicoides (University of Liverpool), modeling of mosquito populations (Reading) and development of novel insecticides and repellent formulations (LSHTM). These specialist expertise were driven by the host organizations for the studies.
Impact Products are still in development.
Start Year 2017
 
Description Supply of Arthropods to External Partners 
Organisation University of Bristol
Country United Kingdom 
Sector Academic/University 
PI Contribution Culicoides nubeculosus, C. sonorensis (biting midge) and Culex pipiens (mosquito) provided as pupae for insecticide, repellent and vector competence trials as a resource. UK supplies conducted under National Capability Grant. Approximately 30 000 insects shipped during reporting period for academic users. All insect production took place at Pirbright and training in maintenance was provided to a worker from FLI (Germany).
Collaborator Contribution Daughter line of C. sonorensis was established at FLI (improving security around sustaining the lines). Training and advice on insect maintenance and handling was provided by Pirbright to LSHTM, Liverpool and Reading. Studies then carried out included investigation of bacteria in Culicoides (University of Liverpool), modeling of mosquito populations (Reading) and development of novel insecticides and repellent formulations (LSHTM). These specialist expertise were driven by the host organizations for the studies.
Impact Products are still in development.
Start Year 2017
 
Description Supply of Arthropods to External Partners 
Organisation University of Greenwich
Department Natural Resources Institute Greenwich
Country United Kingdom 
Sector Academic/University 
PI Contribution Culicoides nubeculosus, C. sonorensis (biting midge) and Culex pipiens (mosquito) provided as pupae for insecticide, repellent and vector competence trials as a resource. UK supplies conducted under National Capability Grant. Approximately 30 000 insects shipped during reporting period for academic users. All insect production took place at Pirbright and training in maintenance was provided to a worker from FLI (Germany).
Collaborator Contribution Daughter line of C. sonorensis was established at FLI (improving security around sustaining the lines). Training and advice on insect maintenance and handling was provided by Pirbright to LSHTM, Liverpool and Reading. Studies then carried out included investigation of bacteria in Culicoides (University of Liverpool), modeling of mosquito populations (Reading) and development of novel insecticides and repellent formulations (LSHTM). These specialist expertise were driven by the host organizations for the studies.
Impact Products are still in development.
Start Year 2017
 
Description Supply of Arthropods to External Partners 
Organisation University of Liverpool
Department School of Veterinary Science Liverpool
Country United Kingdom 
Sector Academic/University 
PI Contribution Culicoides nubeculosus, C. sonorensis (biting midge) and Culex pipiens (mosquito) provided as pupae for insecticide, repellent and vector competence trials as a resource. UK supplies conducted under National Capability Grant. Approximately 30 000 insects shipped during reporting period for academic users. All insect production took place at Pirbright and training in maintenance was provided to a worker from FLI (Germany).
Collaborator Contribution Daughter line of C. sonorensis was established at FLI (improving security around sustaining the lines). Training and advice on insect maintenance and handling was provided by Pirbright to LSHTM, Liverpool and Reading. Studies then carried out included investigation of bacteria in Culicoides (University of Liverpool), modeling of mosquito populations (Reading) and development of novel insecticides and repellent formulations (LSHTM). These specialist expertise were driven by the host organizations for the studies.
Impact Products are still in development.
Start Year 2017
 
Description Supply of Arthropods to External Partners 
Organisation University of Reading
Department School of Biological Sciences Reading
Country United Kingdom 
Sector Academic/University 
PI Contribution Culicoides nubeculosus, C. sonorensis (biting midge) and Culex pipiens (mosquito) provided as pupae for insecticide, repellent and vector competence trials as a resource. UK supplies conducted under National Capability Grant. Approximately 30 000 insects shipped during reporting period for academic users. All insect production took place at Pirbright and training in maintenance was provided to a worker from FLI (Germany).
Collaborator Contribution Daughter line of C. sonorensis was established at FLI (improving security around sustaining the lines). Training and advice on insect maintenance and handling was provided by Pirbright to LSHTM, Liverpool and Reading. Studies then carried out included investigation of bacteria in Culicoides (University of Liverpool), modeling of mosquito populations (Reading) and development of novel insecticides and repellent formulations (LSHTM). These specialist expertise were driven by the host organizations for the studies.
Impact Products are still in development.
Start Year 2017
 
Description Supply of Arthropods to External Partners 
Organisation Wageningen University & Research
Country Netherlands 
Sector Academic/University 
PI Contribution Culicoides nubeculosus, C. sonorensis (biting midge) and Culex pipiens (mosquito) provided as pupae for insecticide, repellent and vector competence trials as a resource. UK supplies conducted under National Capability Grant. Approximately 30 000 insects shipped during reporting period for academic users. All insect production took place at Pirbright and training in maintenance was provided to a worker from FLI (Germany).
Collaborator Contribution Daughter line of C. sonorensis was established at FLI (improving security around sustaining the lines). Training and advice on insect maintenance and handling was provided by Pirbright to LSHTM, Liverpool and Reading. Studies then carried out included investigation of bacteria in Culicoides (University of Liverpool), modeling of mosquito populations (Reading) and development of novel insecticides and repellent formulations (LSHTM). These specialist expertise were driven by the host organizations for the studies.
Impact Products are still in development.
Start Year 2017
 
Description Supply of Arthropods to External Partners 
Organisation Zoological Society of London
Country United Kingdom 
Sector Charity/Non Profit 
PI Contribution Culicoides nubeculosus, C. sonorensis (biting midge) and Culex pipiens (mosquito) provided as pupae for insecticide, repellent and vector competence trials as a resource. UK supplies conducted under National Capability Grant. Approximately 30 000 insects shipped during reporting period for academic users. All insect production took place at Pirbright and training in maintenance was provided to a worker from FLI (Germany).
Collaborator Contribution Daughter line of C. sonorensis was established at FLI (improving security around sustaining the lines). Training and advice on insect maintenance and handling was provided by Pirbright to LSHTM, Liverpool and Reading. Studies then carried out included investigation of bacteria in Culicoides (University of Liverpool), modeling of mosquito populations (Reading) and development of novel insecticides and repellent formulations (LSHTM). These specialist expertise were driven by the host organizations for the studies.
Impact Products are still in development.
Start Year 2017
 
Description Suresh Kuchipudi and Janet Daly 
Organisation Penn State University
Country United States 
Sector Academic/University 
PI Contribution We have hosted a co-supervised PhD student twice at The Pirbright Institute. During these visits we have helped with viral infection of primary cells for analysis of PI3K pathway perturbations and generated new GM influenza viruses to help to understand if the difference in PI3K pathway activity observed between two different avian influenza viruses in avian cells was a result of difference in the NS1 protein. In addition we have provided support to the student regarding the future directions of the project and writing up and presentation of the material. Therefore we have provided reagents, practical expertise and intellectual input.
Collaborator Contribution The collaborators have provided access to data generated in their laboratories and intellectual input to the project.
Impact Publication: doi: 10.1038/srep17999
Start Year 2016
 
Description Suresh Kuchipudi and Janet Daly 
Organisation University of Nottingham
Department School of Veterinary Medicine and Science Nottingham
Country United Kingdom 
Sector Academic/University 
PI Contribution We have hosted a co-supervised PhD student twice at The Pirbright Institute. During these visits we have helped with viral infection of primary cells for analysis of PI3K pathway perturbations and generated new GM influenza viruses to help to understand if the difference in PI3K pathway activity observed between two different avian influenza viruses in avian cells was a result of difference in the NS1 protein. In addition we have provided support to the student regarding the future directions of the project and writing up and presentation of the material. Therefore we have provided reagents, practical expertise and intellectual input.
Collaborator Contribution The collaborators have provided access to data generated in their laboratories and intellectual input to the project.
Impact Publication: doi: 10.1038/srep17999
Start Year 2016
 
Description Swine haplotyping 
Organisation Gift Of Life
Country United States 
Sector Charity/Non Profit 
PI Contribution Prepared and shipped samples
Collaborator Contribution Sample analysis
Impact Data generation. Not multi-disciplinary
Start Year 2016
 
Description The GCRF One Health Poultry Hub 
Organisation Royal Veterinary College (RVC)
Country United Kingdom 
Sector Academic/University 
PI Contribution The focus of the research is to achieve sustainable global intensification of poultry meat and egg production whilst reducing risks to human and animal health and welfare. We are undertaking research to investigate the diversity of avian influenza viruses prevalent in poultry and wild birds in south east Asia.
Collaborator Contribution Royal Veterinary College, London is leading this GCRF One Health Poultry Hub. The project will address the need to meet rising demand for poultry meat and eggs in developing countries, while minimising risk to international public health. Population growth is driving global demand for poultry, meat and egg production; this unfortunately creates conditions in which animal diseases can spread to humans ('zoonoses'). These include bacterial food poisoning and strains with avian influenza with epidemic or pandemic potential. The GCRF One Health Poultry Hub will adopt a 'One Health' approach to the issue of combatting animal-to-human diseases by bringing together a team of laboratory, clinical, veterinary and social scientists. This team will test and evaluate novel interventions. The need for safe poultry production is most urgent in South and South East Asia, so the RVC and its partners will then use their local networks in these regions to put its positive research to immediate use.
Impact Development of international interdisciplinary partnership of 55 investigators from 13 countries to address the emerging global challenges of food security and public health associated with intensification of poultry production.
Start Year 2018
 
Description The Pirbright "Livestock Antibody Hub" funded by Bill & Melinda Gates Foundation 
Organisation The Pirbright Institute
Country United Kingdom 
Sector Academic/University 
PI Contribution Me and my team established collaboration in the "Livestock Antibody Hub" funded ($5.5 million) by the from Bill & Melinda Gates Foundation. Focus of our partnership is to develop techniques and capacity to analyse immune cells (B cells) of cattle, poultry and pigs that produce virus neutralizing antibodies. This research will support rational development of next generation of vaccines and to explore antibody-based passive immunization approaches for treatment and prophylaxis of infectious viral diseases affecting livestock and humans. Our initial focus is to target major animal viral pathogens that cause severe losses within Low and Middle-Income Countries (LMIC) animal production systems including avian influenza virus (AIV), Newcastle disease virus (NDV), infectious bursal disease virus (IBDV), infectious bronchitis virus (IBV) and foot-and-mouth disease virus (FMDV).
Collaborator Contribution This is a collaborative project and partners are providing help in provision of reagents and techniques for analysis of diversity of B cells repertoire that produce infleunza virus-specific antibodies, single cell sequencing and bioinformatic analysis. This project will further strengthen our on-going research collaborations with many research groups in academia as well as with animal health industry (Zoetis, Boehringer Ingelheim Animal Health, Merck, CEVA, Jovac, YeBio, ILRI, Galvmed, The Roslin Institute, HuMabs, AbCellera and Distributed Bio) to take the research outputs from laboratory to the field. The outputs of research will be improved vaccines and diagnostics enabling to reduce the impact of infectious diseases on farm animals, which offer substantial direct and indirect economic, public health, environmental and social benefits to the UK and rest of the world.
Impact Development of passive immunization approaches against avian influenza viruses affecting poultry (project incited in January 2020).
Start Year 2020
 
Description The Roslin Institute 
Organisation University of Edinburgh
Department The Roslin Institute
Country United Kingdom 
Sector Academic/University 
PI Contribution We have been working with several groups using our molecular and genetic data to help understand cattle cellular immunology. We have also hosted a visiting scientist to provide training in molecular techniques and data analysis, This has continued and now includes members of the Centre for Tropical Livestock Health and Genetics where we are engaged in sample sharing and maximising the value of genetic data by linking different sources as much as possible.
Collaborator Contribution Our partners have provided data and reagents as well as intellectual input to help us translate genetic diversity into functional diversity
Impact This collaboration is multi-disciplinary, although largely immunology and associated genetics is also involves bioinformatics
Start Year 2011
 
Description The effect of the microbiota on immunity to swine infleunza 
Organisation University of Surrey
Country United Kingdom 
Sector Academic/University 
PI Contribution We wish to establish the gut and nasal microbiome in normal healthy pigs and how this is affected by influenza infection. We have collected gut samples from pigs at different stages of infection, as well as nasal swabs which we will provide to our collaborators in Surrey.
Collaborator Contribution Our collaborators in Surrey will perform the sequencing and bioinformatics analysis to determine the microbial communities present in the samples.
Impact No outcomes yet - we are in the process of analysing the samples.
Start Year 2017
 
Description Thomas Bowden - STRUBI - University of Oxford - Glycoprotein structure collaboration 
Organisation University of Oxford
Country United Kingdom 
Sector Academic/University 
PI Contribution Provision of data and advice about the biological properties of viral glycoproteins, e.g. their fusion capacity with various receptors, their role in determining host-range
Collaborator Contribution Advice and technical support at the structural level supporting future project development and design
Impact No outcomes as yet
Start Year 2018
 
Description Trevor Sweeney - University of Cambridge PhD Studentship 
Organisation University of Cambridge
Country United Kingdom 
Sector Academic/University 
PI Contribution Development of PhD studentship project in collaboration with Dr. Sweeney's lab at Cambridge and application at Pirbright.
Collaborator Contribution Development of PhD studentship project and application at Cambridge.
Impact The PhD studentship has been funded and is currently been advertised.
Start Year 2018
 
Description University of Glasgow collaborations (Sam Wilson and Massimo Palmarini) 
Organisation University of Glasgow
Department MRC - University of Glasgow Centre for Virus Research
Country United Kingdom 
Sector Academic/University 
PI Contribution Scientific discussion and reagent sharing contributing to grant applications and active research projects
Collaborator Contribution Scientific discussion and reagent sharing contributing to grant applications and active research projects
Impact Successful MRC grant application
Start Year 2015
 
Description University of Liverpool Proteomics Collaboration 
Organisation University of Liverpool
Country United Kingdom 
Sector Academic/University 
PI Contribution Generation of samples to analyse the membranous proteome of cells infected with paramyxoviruses.
Collaborator Contribution Proteomic analysis of samples and technical support. Access to previous data-sets and expertise.
Impact Preliminary data sets only to date.
Start Year 2018
 
Description Wilhelm Gerner 
Organisation University of Veterinary Medicine Vienna
Country Austria 
Sector Academic/University 
PI Contribution Our lab hosted Dr Gerner who brought samples for RNA-seq. My lab planned and executed the sequencing and is contributing to the analysis.
Collaborator Contribution Dr Gerner did all the animal work and cell sorting to produce the samples reading for RNA-seq
Impact None as yet, analysis is ongoing
Start Year 2019
 
Title Chicken IFITMs 
Description The present invention provides an avian cell in which the expression or activity of one or more of the following genes, or a homologue thereof: Chicken IFITM1 (SEQ ID No. 1); Chicken IFITM2 (SEQ ID No. 2) and Chicken IFITM3 (SEQ ID No. 3) is reduced. The invention also provides methods for passaging viruses in avian cells, embryos and/or avian cell lines which have reduced expression of one or more IFITM genes and methods which involve investigating the sequence of one or more of the following genes, or a homologue thereof: Chicken IFITM1 (SEQ ID No. 1); Chicken IFITM2 (SEQ ID No. 2) and Chicken IFITM3 (SEQ ID No. 3). 
IP Reference WO2014195692 
Protection Patent granted
Year Protection Granted 2018
Licensed Commercial In Confidence
Impact The present invention provides an avian cell in which the expression or activity of one or more of the following genes, or a homologue thereof: Chicken IFITM1 (SEQ ID No. 1); Chicken IFITM2 (SEQ ID No. 2) and Chicken IFITM3 (SEQ ID No. 3) is reduced. The invention also provides methods for passaging viruses in avian cells, embryos and/or avian cell lines which have reduced expression of one or more IFITM genes and methods which involve investigating the sequence of one or more of the following genes, or a homologue thereof: Chicken IFITM1 (SEQ ID No. 1); Chicken IFITM2 (SEQ ID No. 2) and Chicken IFITM3 (SEQ ID No. 3). This patent has enabled us to leverage funding for continued work.
 
Description Talk Presented at 2nd International Congress of Veterinary Microbiology, 16th -19th October 2018. Sherwood Breezes Resort-Lara, Antalya, Turkey. 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Conference discussed improving prevention and control of viral diseases of poultry.
Year(s) Of Engagement Activity 2018
 
Description "Challenges for Poultry Industry". Organised by Fakieh Poultry at the Ritz-Carlton Hotel in Jeddah, Saudi Arabia, 2nd -3rd December 2018, 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Conference discussed prevention and control of infectious diseases affecting poultry production.
Year(s) Of Engagement Activity 2018
 
Description "Interactive session with poultry stakeholders". Title: Overview of Avian Influenza group Research at The Pirbright Institute. Presented at University of Veterinary and Animal Sciences (UVAS) Lahore, Pakistan, 3rd August 2018. 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact The workshop discussed the economic impacts and prevention strategies against avian influenza viruses affecting poultry production and zoonotic infections.
Year(s) Of Engagement Activity 2018
 
Description 1st Research Coordination Meeting (RCM) of the Coordinated Research Project (CRP) on "Generic approach for the development of genetic sexing strains for SIT applications" Vienna, 7-11 October 2019 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact FAO/IAEA Coordinated Research Project on exploring genetic and molecular methods of sex separation in insect pests; aimed at promoting specific areas of research, exchange of ideas and networking among the experts in the field.
Year(s) Of Engagement Activity 2019
 
Description 2. Overview of Avian Influenza group Research at The Pirbright Institute. Presented at "Interactive session with poultry stakeholders" at University of Veterinary and Animal Sciences (UVAS) Lahore, Pakistan, 3rd August 2018. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Dissemination of research Project outcomes These include presenting research data for vaccine seed strains with emergent field avian influenza variants; The zoonotic risk posed by emerging avian influenza viruses circulating in the wild birds and poultry. Impact of virus evolution on vaccine efficacy and persistence in poultry.
Year(s) Of Engagement Activity 2018
 
Description 360 degree Lab Animal Tour (and film) of The Plowright Building, The Philip Mellor Insectary, and Animal Isolation Facilities 
Form Of Engagement Activity Engagement focused website, blog or social media channel
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact The website provides unique access to the animal research facilities at The Pirbright Institute which support its world-leading scientific research into livestock viral diseases and helps save countless animal and potentially human lives, globally. Produced and filmed by Understanding Animal Research, the Lab Animal Tour was filmed at Pirbright, and at three other research centres, and provides the viewer with an interactive 360 virtual tour of the animal facilities.
Year(s) Of Engagement Activity 2017
URL http://www.labanimaltour.org/pirbright
 
Description A talk at a symposium entitled: Using the 3Rs to support good science 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact I gave a talk at a Symposium held at The Pirbright Institute entitled: "Using the 3Rs to support good science". The talk was entitled: "A chicken primary B cell culture model to study the pathogenesis and improve the control of immunosuppressive viruses of poultry"
Year(s) Of Engagement Activity 2017
 
Description A talk at the British Poultry Diseases Group meeting 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact The Poultry Diseases Group meet quarterly and is comprised of representatives from private veterinary practices, vaccine and pharmaceutical industries, and the Animal and Plant Health Agency (APHA). I gave a talk at one of the meetings outlining my research to date and future directions.
Year(s) Of Engagement Activity 2017
 
Description Amin Asfor presented the findigs of the project at the Microbiology Society Annual meeting, 2019 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Amin Asfor presented the findigs of the project at the Microbiology Society Annual meeting, 2019. Title: in vivo and ex vivo models of Infectious Bursal Disease Virus (IBDV) in inbred chicken lines differing in their resistance to the disease.
Year(s) Of Engagement Activity 2019
 
Description Amin Asfor presented the work at the NC3Rs Fellows Day 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Amin Asfor presented a poster at the NC3Rs Fellows Day entitled: Chicken primary B cell culture model to study the pathogenesis and improve the control of immunosuppressive viruses of poultry
Year(s) Of Engagement Activity 2019
 
Description Amy Lewis, The Scientist, 15/08/2017 re gene drive and Predator Free NZ 2050 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Media (as a channel to the public)
Results and Impact Provided information and quotes for article on potential use of gene drives as part of New Zealand effort to eradicate invasive predators
Year(s) Of Engagement Activity 2017
URL https://www.the-scientist.com/?articles.view/articleNo/50180/title/Driving-Down-Pests/
 
Description Ash Manor GCSE Mentoring 2018 - BA 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Stimulating increased interest in research
Year(s) Of Engagement Activity 2018
 
Description Ash Manor school challenge week science workshops 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Provide information on research activity at The Pirbright Institute and informing on pathogens infecting farmed animals.
Year(s) Of Engagement Activity 2019
 
Description BBC Today programme 24/01/2019 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Media (as a channel to the public)
Results and Impact Interview on BBC Today programme regarding potential development and use of gene drives in mice
Year(s) Of Engagement Activity 2019
URL https://www.bbc.co.uk/sounds/play/m00025bt
 
Description BBSRC Partnering Seminar, University of Liverpool, Tamilnadu Veterinary and Animal Science University 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Talk presented entitled " Molecular determinants of antigenicity of H7 and H9 avian Influenza viruses". The research data shared with the colleagues working to reduce the impact of avian infectious diseases on poultry production. The discussion led to more collaboration and work together, acquiring reagents and protocols for further research and development activities.
Year(s) Of Engagement Activity 2019
 
Description BBSRC website article: Halloween 
Form Of Engagement Activity Engagement focused website, blog or social media channel
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact Article on BBSRC website: Halloween Creepy Crawlies
Year(s) Of Engagement Activity 2017
URL http://www.bbsrc.ac.uk/news/fundamental-bioscience/2017/171031-f-uk-creepiest-creepy-crawlies/
 
Description BIotech YES 2019 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Postgraduate students
Results and Impact Gave a talk at the BBSRC Biotechnology Young Entrepreneurs' Scheme event at Syngenta (Jealott's Hill)
Year(s) Of Engagement Activity 2019
 
Description Big Band Science Event- AH, ER, LVC, MdP 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Stimulating increased interest in science and research
Year(s) Of Engagement Activity 2019
 
Description Big Bang Fair 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Schools
Results and Impact The Big Bang Near Me is a programme of regional and local Big Bang Fairs that take place all across the UK. Together with The Big Bang UK Young Scientists & Engineers Fair and The Big Bang Competition, it forms part of the wider Big Bang programme, bringing science, technology, engineering and maths (STEM) to life for young people
Year(s) Of Engagement Activity 2019
URL https://nearme.thebigbangfair.co.uk/about/
 
Description Big Bang Fair South East - RC 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Regional science festival/event - changes in public attitudes and stimulating increased interest in research
Year(s) Of Engagement Activity 2018
 
Description Big Bang UK- Jennifer Simpson 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Schools
Results and Impact Big Bang UK is a science competition. JS acted as moderator for final projects submitted by students from across the UK.
Year(s) Of Engagement Activity Pre-2006,2019
 
Description Biotech YES, Syngenta Jealott's Hill, 05 Oct 2017 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Postgraduate students
Results and Impact Teams of postgraduate students from a number of universities and institutes attended talk as part of Biotech YES (Young Entrepreneurs' Scheme). Follow-up discussion influenced several aspects of their approach to entrepreneurship both within the competition and more generally
Year(s) Of Engagement Activity 2014,2015,2016,2017
 
Description Blenheim High School Fair - DB 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Team members attended sessions at a local high school with secondary school children and those in the 6th form to talk about paths into scientific careers, sharing their experiences of working at The Pirbright Institute. Approximately 300 students attended and it stimulated increased interest in science and research.
Year(s) Of Engagement Activity 2019
 
Description Bluetongue and Wildlife (15 minute presentation on Epizootic Haemorhagic Disease Virus), (July/Pirbright, UK) 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Policymakers/politicians
Results and Impact Policymakers reported greater interest in deer-associated diseases including EHDV and BTV
Year(s) Of Engagement Activity 2018
 
Description Brownies-AH 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Talking to the Brownies about science and their careers
Year(s) Of Engagement Activity 2019
 
Description CABI_Research activity of Avian Immunology Group at The Pirbright Institute 
Form Of Engagement Activity Engagement focused website, blog or social media channel
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Media (as a channel to the public)
Results and Impact Release of research activity within Avian Immunology group at The Pirbright Institute
Year(s) Of Engagement Activity 2018
 
Description CBD Open-ended online forum on synbio 06-10/2017 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Policymakers/politicians
Results and Impact UN Convention on Biodiversity. Policy- discussion on synthetic biology: nominated participant in the Open Ended Online Forum on Synthetic biology as established in decision XII/24 of the Conference of the Parties to the Convention on Biological Diversity (COP) and extended in COP decision XIII/17 which ran from June to Oct 2017 (approx.)
Year(s) Of Engagement Activity 2017
URL http://bch.cbd.int/synbio/open-ended/discussion/
 
Description CNN interview 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Media (as a channel to the public)
Results and Impact Interview with CNN journalist. Journalist later followed up asking me to check and clarify some issues.
Year(s) Of Engagement Activity 2019
 
Description Cafe Scientifique, Bradfield School, West Berkshire: "How insects spread viruses" 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact Cafe Scientifique is a public forum for exploring the latest ideas in science and technology. Meetings take place in cafes, bars, restaurants and even theatres, but always outside a traditional academic context. I presented to an audience of the general public and answered questions.
Year(s) Of Engagement Activity 2017
 
Description Cafe Scientifique, Reading: "How insects spread viruses" 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact Cafe Scientifique is a public forum for exploring the latest ideas in science and technology. Meetings take place in cafes, bars, restaurants and even theatres, but always outside a traditional academic context. I presented to an audience of the general public and answered questions.
Year(s) Of Engagement Activity 2007,2017
 
Description Camberley Library Fun Palace 2018 - JM 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact Changes in public attitudes and stimulating increased interest in research
Year(s) Of Engagement Activity 2018
 
Description Caroline Seydel Genetic Literacy Project re GM mosquitoes/gene drive/Zika 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Media (as a channel to the public)
Results and Impact Provided background material and quotes for article on GM mosquitoes and control of Zika virus.
Year(s) Of Engagement Activity 2017
URL https://geneticliteracyproject.org/2018/01/29/waging-war-mosquito-split-gene-drive-hinder-spread-zik...
 
Description Centre of excellence for research on avian diseases (CERAD) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact I was invited to present my work at the UK-China Centre of excellence for research on avian diseases (CERAD) meeting attended by researchers from UK, China and Thailand. There was lots of discussion about the research and future directions, including potential collaborations.
Year(s) Of Engagement Activity 2017
 
Description Cheltenham Science Festival 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Institute stand at the festival covering the Institute science in general, and transmission and gene editing to control disease, in particular.
Year(s) Of Engagement Activity 2018
 
Description Cheltenham Science Festival 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Stimulating increased interest in science and research
Year(s) Of Engagement Activity 2019
 
Description Cheltenham Science Festival 2018 - JA 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Major science festival - changes in public attitudes and stimulating increased interest in research
Year(s) Of Engagement Activity 2018
 
Description Cheltenham Science Festival 2018 - JM 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Major science festival - changes in public attitudes and stimulating increased interest in research
Year(s) Of Engagement Activity 2018
 
Description Cheltenham Science Festival 2018 - JP 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Major national science festival - changes in public attitudes and stimulating increased interest in research
Year(s) Of Engagement Activity 2018
 
Description Cheltenham Science Festival 2018 - RC 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Major science festival - changes in public attitudes and stimulating increased interest in research
Year(s) Of Engagement Activity 2018
 
Description Cheltenham Science Festival 2018 - SV 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Display at major science festival: changes in public attitudes and stimulating increased interest in research
Year(s) Of Engagement Activity 2018
 
Description Cheltenham Science Festival 2018-DP 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Major national science festival - changes in public attitudes and stimulating increased interest in research
Year(s) Of Engagement Activity 2018
 
Description Cheltenham Science Festival- AH, ER, JC 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Present at the Pirbright stand to discuss science with the public
Year(s) Of Engagement Activity 2019
 
Description Cheltenham Science festival - LB 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact National science festival which Team members attended to demonstrate to the public how scientists at The Pirbright Institute can work to contain and control viral outbreaks. Over 500 people accessed the information and this stimulated increased interest in science and research.
Year(s) Of Engagement Activity 2019
 
Description Cheltenhan Science Festival - Dana Perry 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact DP helped to run a stand at the festival, questions from the general public stimulated increased interest in science and research and increased the visibility of The Pirbright Institute.
Year(s) Of Engagement Activity 2019
 
Description Combating avian influenza through systematic analysis of antigenic drift, genetic variation, and development of novel diagnostic tools and vaccines. Presented at as PI of the ZELS project at ZELS grant holders and Stakeholders meeting in Hanoi, Vietnam 23th-24th January 2017. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Engagement and dissemination of research outcome to representatives of research funding bodies such as Department for International Development (DFID), Medical Research Council (MRC) and the researchers working on different research projects funded by BBSRC under Zoonoses and Emerging Livestock Systems (ZELS).
Year(s) Of Engagement Activity 2018
 
Description Conference session organiser at MMC2017 - Host Pathogen Interactions 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Chair for the Imaging Host-Pathogen Interactions session run at MMC2017 (and in previous years). Session usually attracts approx. 30 delegates from national/international backgrounds, mostly academic. Talks sparked discussions and networking opportunities.
Year(s) Of Engagement Activity 2012,2014,2015,2017
 
Description Coronavirus lecture MSc students (Surrey) 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Postgraduate students
Results and Impact A lecture was presented on the replication, cellular interactions and pathogenesis of coronaviruses to 15-20 MSc students from University of Surrey. The students were engaged and interested, answering and asking questions during the session.
Year(s) Of Engagement Activity 2016,2018
 
Description DARPA visit April 2017 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Supporters
Results and Impact Hosted visit by US funding agency programme manager to The Pirbright Institute
Year(s) Of Engagement Activity 2017
 
Description Deimante Lukosaityte: Microbiology Society conference 2019. Poster presentation: Chicken protection against H9N2 virus by passive immunization 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Described techniques for development of next generation of vaccines against viral diseases affecting animals and humans.
Year(s) Of Engagement Activity 2019
 
Description Demonstrating Mathematics (Angita Shrestha) 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Postgraduate students
Results and Impact Angita Shrestha (PhD student within Avian Influenza group) provided help in a Maths session to the new Oxford Doctoral Training Porgramme (DTP) cohort.
Year(s) Of Engagement Activity 2018
 
Description Determinants of antigenicity of H9 Avian Influenza Viruses. UK-China Swine and Poultry Workshop.17-18 June 2019, The Pirbright Institute, UK. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact The talk and the discussion on the improvement of disease control systems (vaccines and diagnostics) to prevent avian influenza viruses infection in poultry and zoonotic infections in humans.
Year(s) Of Engagement Activity 2019
 
Description Diamond (TT) 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Diamond Light Source Open Day - explaining to general public the importance of structural biology and microscopy for understanding viruses and designing improved vaccines.
Year(s) Of Engagement Activity 2019
 
Description Diamond Light Source Open day - HJM 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact A public open day for Diamond Light Source, which Pirbright attended as a user of the facility to showcase research being performed at Pirbgith in collaboration with Diamond. There was a lot of interest in the fundamental research and sicussion about future plans and how it fit with the facility at Diamond.
Year(s) Of Engagement Activity 2019
 
Description Diamond open day 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Over 300 people visited our stand at the open day, in which we described the importance of our work for food security and public health. Children from all ages were involved and learned about viruses and played with the huge virus particle that we had produced. One of the major impact was that we described to several people why we work on some viruses that we do not have them in the UK, and the importance of the work for the UK and UK economy and public health was explained.
Year(s) Of Engagement Activity 2017
URL http://www.diamond.ac.uk/Public/VisitUs.html
 
Description Diego Friere Pesquisa Fapesp 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Media (as a channel to the public)
Results and Impact Interview with journalist leading to article regarding release of transgenic mosquitoes into the environment to control dengue and other diseases
Year(s) Of Engagement Activity 2019
URL https://revistapesquisa.fapesp.br/en/2020/02/05/jacobina-controversy/
 
Description Discuss gene drives with NZ Parliamentary Commissioner 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Policymakers/politicians
Results and Impact Met NZ Parliamentary Commissioner to provide background information on, and discuss, gene drive systems. New Zealand is considering genetic and other control methods for invasive pests.
Year(s) Of Engagement Activity 2018
 
Description EM-UK 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Co-organiser and speaker for UKRI supported network for electron microscopists in the UK. I was one (or two) of the founder members. Meeting has been running for 5 years. Increased the visibility of The Pirbright Institute.
Year(s) Of Engagement Activity 2020
 
Description EM-UK: A national network for electron microscopists 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Founder and Chair of EM-UK, a national network (with international participants) for electron microscopists. Includes a one or two day meeting with invited speakers, and email discussion list for questions, comments, advertising courses, advertising jobs. It has proved very popular with over 200 people registering for the discussion list, and 90+ delegates at each meeting. Administration is handled by the Royal Microscopical Society.
Year(s) Of Engagement Activity 2016,2017,2018
 
Description EMBO Workshop: Molecular and population biology of mosquitoes and other disease vectors: vector and disease control 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Presentation on "Sex determination pathway in Anopheles gambiae as a target for mosquito control" given during the meeting held on 22 - 26 July 2019 in Kolymbari, Greece. Following a presentation a request was made to give an informal seminar at the Department of Life Sciences, Imperial College, London.
Year(s) Of Engagement Activity 2019
URL https://meetings.embo.org/event/19-mosquitoes
 
Description Edinburgh Science Festival 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Talk and panel event at Edinburgh Science Festival "The Buzz about GM Insects". Sparked questions and discussions afterwards including some career questions.
Year(s) Of Engagement Activity 2019
 
Description Elle Campbell Presented her data at the 2019 Microbiology Society Annual Meeting 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Elle Campbell gave an oral presentation at the 2019 Microbiology Society Annual Meeting on the work we were doing with fluorescently tagged IBDVs and virus factory movement.
Year(s) Of Engagement Activity 2019
 
Description Encouraging women into science and engineering STEM. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact MH Visited Warwick School, Redhill to talk to secondary students about careers in STEM. A very positive outcome and well received.
Year(s) Of Engagement Activity 2018
 
Description European Society Vector Ecology (Invited Chair for two sessions and speaker for 15min talk); (October/Palermo, Sicily) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Chaired two sessions, one on vector-borne diseases, one on scientific networks. Latter included a discussion with early career researchers. Main outcome was coordination between active networks including The Gnatwork.
Year(s) Of Engagement Activity 2018
URL http://www.sove.org/European%20SOVE%20folder/greecescientificprogram/palermo_Scientific_program_revi...
 
Description Evolution of H9N2 avian influenza virus under immune pressure. Presented at 10th International Symposium on Avian Influenza, 15th - 18th April 2018, The Grand Hotel, Brighton, UK. 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact The primary aim of the workshop was to forge long-term research partnerships between early-career researchers, livestock industry and national disease control authorities in the UK and the Philippines. In addition, the activity hopes provide information about the emerging and next generation diagnostic and vaccine strategies and their utilization to reduce the impact of viral diseases on livestock and poultry.
Year(s) Of Engagement Activity 2019
URL https://www.slideshare.net/zerep_cire/2019-newton-agham-researcher-links-workshop-vaccines-and-diagn...
 
Description Ewen Callaway Nature 08/01/2018 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Media (as a channel to the public)
Results and Impact Provided expert background information for article in Nature regarding engineering species barriers, described by journalist as "very helpful" in followup email.
Year(s) Of Engagement Activity 2018
 
Description FAO/IAEA Coordinated Research Programme on methods of sex separation in mosquitoes; Research coordination meeting Bangkok 19-23 February 2018 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact FAO/IAEA Coordinated Research Programme on exploring genetic, molecular, mechanical, and behavioral methods of sex separation in mosquitoes; aimed at promoting specific areas of research, exchange of ideas and networking among the experts in the field.
Year(s) Of Engagement Activity 2013,2015,2016,2018
 
Description FLI - presentation 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Postgraduate students
Results and Impact Presentation of current research to the FLI - Germany to build collaborations 19/20 November 2018
Year(s) Of Engagement Activity 2018
 
Description Facebook Live: Infectious Disease Portrayals in Pop Culture 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact Infectious diseases are a frequent topic in movies. The CDC has used zombies to talk about infectious disease and disaster preparedness, and infotainment has flamed fears of Ebola. I took part in a Facebook Live chat organised by the AAAS Public Engagement team about why infectious diseases are so often the topic of pop culture discussions; what movies, books, and other pop culture gets right and wrong about infectious disease; and how scientists can use pop culture as a jumping-off point for public engagement. The chat was broadcast live on the AAAS Facebook page.
Year(s) Of Engagement Activity 2017
URL https://www.aaas.org/event/facebook-live-infectious-disease-portrayals-pop-culture
 
Description Farnborough College Apprenticeship Fair- 20th March 2018 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Careers fair attended by approx. 100 school children. Pirbright demonstrated the multiple ways that research organisations can provide jobs including scientists, engineers and computer operators.
Year(s) Of Engagement Activity 2018
 
Description Farnborough Sixth Form College Careers Fair - Chris Chiu 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact CC engaged at a careers day, and answered questions presented by sixth form students about working in science. Increased the visibility of The Pirbright Institute.
Year(s) Of Engagement Activity 2019
 
Description Farnborough futures careers event - HJM 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Schools
Results and Impact The careers event aimed to introduce the range of careers available at Pirbright. In addition I discussed my own career progression and highlighted the range of possible careers that I could have entered. Finally, I discussed some details of the research with interested students. The students had a wide range of backgrounds and career aims but were interested to hear about the Institute and the sort of work opportunities available. Several of them planned to follow up by investigating future job opportunities at Pirbright.
Year(s) Of Engagement Activity 2019
 
Description Food security and the control of infectious disease in livestock 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact A workshop was organized by myself at St Catherine's College Oxford for the duration of three days. Representative from industry, funding agencies and media (publishers) attended this workshop. Over 20 delegates from Egypt and 20 delegates from the UK attended this meeting. The purpose of this workshop/ meeting was to promote international collaborations, provide training for undergraduate students, promote the importance of managing and controlling infectious disease in livestock and public health.
Year(s) Of Engagement Activity 2016
 
Description Gene Drive Resarch Sponsors and Supporter's Forum meeting Montreal Sept 2018 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Supporters
Results and Impact Forum met to discuss the state of the art and future considerations for gene drive research. Ongoing deliberations including specific follow-up meetings.
Year(s) Of Engagement Activity 2018
 
Description Gnatwork Website 
Form Of Engagement Activity Engagement focused website, blog or social media channel
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact New website established to act as focalpoint of project.
Year(s) Of Engagement Activity 2018
URL http://www.gnatwork.ac.uk/
 
Description Guest lecturer MSc Imaging course at University of Copenhagen 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact 25 internal students from University of Copenhagen visited the core imaging facility for three days during which time I delivered lectures and interacted with the students during practical workshops. This sparked questions and discussion relating to their individual projects.
Year(s) Of Engagement Activity 2018
 
Description Guest lecturer for PhD course in EM, University of Copenhagen 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Workshop for delegates including lectures, seminars and practical sessions. Outcomes are that delegates are able to make informed decisions regarding microscopy techniques about their future projects, and perform sample preparation and operate instruments. Delegates could ask me for advice regarding their projects which sparked questions and discussion within the group on a wide range of multi-disciplinary subjects. Increase visibility of The Pirbright Institute
Year(s) Of Engagement Activity 2019
 
Description H9N2 avian influenza viruses: impact of evolutionary changes on virus antigenicity, receptor binding and zoonotic potential. Presented at "Conference on Animal Infectious Diseases and Human Health" held at The Veterinary Biotechnology Branch of Chinese Association of Animal Science and Veterinary Medicine & the Veterinary Immunology Branch of Chinese Society for Immunology. August 7th-10th, 2018 Harbin, China. 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Focus of the conference was to improve control against infectious diseases affecting poultry and livestock.
Year(s) Of Engagement Activity 2018
 
Description H9N2 avian influenza viruses: impact of evolutionary changes on virus antigenicity, receptor binding and zoonotic potential. Presented at "Conference on Animal Infectious Diseases and Human Health" held at The Veterinary Biotechnology Branch of Chinese Association of Animal Science and Veterinary Medicine & the Veterinary Immunology Branch of Chinese Society for Immunology. August 7th-10th, 2018 Harbin, China. 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Dissemination of research outcomes to prevent and control of infectious diseases of animals and humans.
- Avian influenza virus evolution and efficacy.
-
Year(s) Of Engagement Activity 2018
 
Description Holly Kernot, Veterinary Times, 13/06/2017 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Media (as a channel to the public)
Results and Impact Discussed potential of manipulating mosquito sex determination genes as tools for novel mosquito genetic control approaches.
Year(s) Of Engagement Activity 2017
URL https://www.vettimes.co.uk/news/study-to-be-transformational-in-mosquito-control-strategies/
 
Description Holt School Surrey. Bee meadow & A-level outreach Talk. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact I discussed the importance of pollinators for biodiversity and food security with the younger students. I also discussed careers in science with A-Level students. There was good interaction and many questions surrounding both topics.
Year(s) Of Engagement Activity 2018
 
Description Human Infection Challenge Models Network 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Policymakers/politicians
Results and Impact HIC-Vac is an international network of researchers who are developing human infection challenge (HIC) studies to accelerate the development of vaccines against pathogens of high global impact. The goal is to foster an engaged and interactive community of international researchers to promote open sharing of knowledge and expertise, generate new ideas, support and share best practice, and form new cross-discipline collaborations.
Year(s) Of Engagement Activity 2018
 
Description IUCN Technical Subgroup on Synthetic Biology and Biodiversity Conservation (meeting 12-15 April and report) 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Policymakers/politicians
Results and Impact Participated as member in Technical Subgroup on Synthetic Biology and Biodiversity Conservation of International Union for Conservation of Nature (IUCN). Meetings, online discussion and ultimately a report to Task Force and will ultimately go to IUCN General Assembly.
Year(s) Of Engagement Activity 2018,2019
 
Description Identification of antigenic epitopes to broaden and enhance the efficacy of avian influenza vaccines. Presented at 6th Animal Vaccines and immune Adjuvant Technologies Salon & Advanced seminar, Shandong-Binzhou Animal Science and Veterinary Medicine Academy, China, 18-20 October 2019. 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact The talk and the discussion was the best practices to improve control systems (vaccines and diagnostics) for prevention of avian influenza viruses infection in poultry and zoonotic infections in humans.
Year(s) Of Engagement Activity 2019
 
Description Imagineering Fair 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact Talked to the general public about Pirbright science and Beekeeping
Year(s) Of Engagement Activity 2019
 
Description Immuno-pathobiology of H9N2 avian influenza viruses: looking at how the viruses evolve and persist in poultry. Presented at "Techniques for Healthy Farming and Diseases Prevention & Control of Livestock and Poultry. Beijing, August 13th-15th, 2018. 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Dissemination of research outputs. Improvement of vaccines and diagnostics.
Year(s) Of Engagement Activity 2018
 
Description Improving vaccines and diagnostics for Avian influenza viruses affecting poultry. Presented at the International Poultry Expo "Poultry Science Conference", Lahore, Pakistan, 13-15 September 2019. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact The talk and the discussion was the best practices to improve control systems (vaccines and diagnostics) for prevention of avian influenza viruses infection in poultry and zoonotic infections in humans.
Year(s) Of Engagement Activity 2019
 
Description InFocus article (Jennifer Simpson) 
Form Of Engagement Activity A magazine, newsletter or online publication
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact InFocus review article for EM-UK meeting (2017)
Year(s) Of Engagement Activity 2017
 
Description Influenza update 2018 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Postgraduate students
Results and Impact Approx. 70 scientists attended a Influenza update meeting at which our group presented an oral presentation and poster presentation on our work. These actions resulted in establishment of new collaborations for ongoing work and the initiation of a joint grant writing exercise.
Year(s) Of Engagement Activity 2018
 
Description Influenza update meeting (Pengxiang Chang) 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact presented work entitled: Antigenic characterization of avian influenza H7N9 virus by in vitro immune escape mutant selection method.
Year(s) Of Engagement Activity 2018
 
Description InnovSur : Conference in IRD Montpellier on Insect Vector Surveillance 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Working group on standardisation of techniques for surveillance of insect vectors. Included break out group brainstorming and subsequent commentaries. Total audience approximately 110 people for main lectures.
Year(s) Of Engagement Activity 2018
URL https://www.alphavisa.com/isessah-innovsur/2018/
 
Description Innovate Guildford 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Institute stand at Innovate Guildford science festival. Visitors to stand able to discuss issues related to animal diseases and their control (with a particular focus on influenza and on use of genetic modification technologies)
Year(s) Of Engagement Activity 2017
 
Description Innovate Guildford (Angita Shrestha): 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact GENERAL SCIENCE
Year(s) Of Engagement Activity 2019
 
Description Innovate Guildford - DB & KC 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Public science event where team members participated on a Pirbright Institute stand which contained activities to help the public understand how scientists can help to contain and control viral outbreaks. Over 500 members of the general public attended which stimulated increased interest in science and research and led to requests for more information.
Year(s) Of Engagement Activity 2019
 
Description Innovate Guildford 17 March 2018 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact Talk and exhibit at Innovate Guildford - >100 for talk, probably >500 for exhibit. Both sparked questions and discussion afterwards.
Year(s) Of Engagement Activity 2018
 
Description Innovate Guildford 2018 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Institute stand at the Innovate Guildford event, covering the work of the Institute in general and genetic modification through gene editing specifically.
Year(s) Of Engagement Activity 2018
URL https://www.guildford.gov.uk/IG18
 
Description Innovate Guildford 2018 - JM 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Regional science/innovation event - changes in public attitudes and stimulating increased interest in research
Year(s) Of Engagement Activity 2018
 
Description Innovate Guildford 2018 - SB 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Regional science festival - changes in public attitudes and stimulating increased interest in research
Year(s) Of Engagement Activity 2018
 
Description Innovate Guildford 2018 - SV 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Display at regional science/innovation festival - changes in public attitudes and stimulating increased interest in research
Year(s) Of Engagement Activity 2018
 
Description Innovate Guildford, ND 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Presented at the Pirbright Institute stand discussing science performed by the Institute with the general public. The stand focussed on an exhibit on avian influenza virus but more general virology topics were also discussed including our research.
Year(s) Of Engagement Activity 2017
 
Description Innovate Guilford 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact Dr Broadbent gave a talk at 'Innovate Guilford' Festival outlining the work we are doing with Avian Virus Vaccines, including those against IBDV. The aim was to raise public awareness on different avian viruses, and genetically modified vaccines.
Year(s) Of Engagement Activity 2017
 
Description Innovate Guilford-AH, AA, ER, EL 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact General science festival engaging the public in the research undertaken at Pirbright
Year(s) Of Engagement Activity 2019
 
Description Insect Festival 2017 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Ran a stand on blood-sucking arthropods and their role in the transmission of disease at the Royal Entomological Society's Insect Festival. Talked to public, answered questions. Lots of enquiries about ticks, midges, mosquitoes and large biting flies (particularly horseflies).
Year(s) Of Engagement Activity 2017
URL https://www.yorkmuseumstrust.org.uk/whats-on/events/insect-festival-2017/
 
Description Institute stand at Surrey Vet School open day 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Schools
Results and Impact Stimulating increased interest in science and research
Year(s) Of Engagement Activity 2019
 
Description International Avian Respiratory Disease Conference 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact Michael presented his research at the 2018 International Avian Respiratory Disease Conference in the US to an audience of avian researchers and representatives form the poultry veterinary vaccine industry. This sparked discussion and potential future collaborations
Year(s) Of Engagement Activity 2018
 
Description International Day of Women and Girls in Science 
Form Of Engagement Activity Engagement focused website, blog or social media channel
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact I gave a quote as a STEM ambassador about why I think it is important to make sure women and girls have the same opportunities in STEM. This was shared on social media to celebrate International Day of Women and Girls in Science, a UN-led initiative.
Year(s) Of Engagement Activity 2019
 
Description International Women's Day video for social media 
Form Of Engagement Activity Engagement focused website, blog or social media channel
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact Three team members were interviewed about their careers in science for International Women's Day. The videos were posted on Pirbright's website, Facebook and Twitter to celebrate what we love about working in science.
Year(s) Of Engagement Activity 2018
 
Description Interview with BBC journalist for article on viral emergence in new species 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact I was interviewed by Jonathon Ball who was writing an article for the BBC on the emergence of morbilliviruses in new hosts and the importance of vaccination. I helped him to contextualise the story and put him in touch with my collaborators.
Year(s) Of Engagement Activity 2020
URL https://www.bbc.co.uk/news/science-environment-50839868
 
Description Invitation to give keynote talk on 'Control of avian diseases: Challenges and opportunities' 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Gave the Keynote talk on 'Control of avian diseases: Challenges and opportunities' at this International Symposium organized with an aim to gather international virology experts from both human and veterinary fields to share research experience, update findings and contribute their expertise through presentation and interactive discussion. The topics will cover a wide range of virology research common in both human and animal aspects. In addition, technological platforms such as vaccine development, and novel assays will be emphasized and addressed.
Year(s) Of Engagement Activity 2018
URL http://www.biotec.or.th/virus-symposium2018/index.php
 
Description Invited Chair at European Congress of Entomology (Naples,July) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Chaired session on vector-borne diseases at meeting and subsequent question sessions with 15 minute presentation on Culicoides biting midges.
Year(s) Of Engagement Activity 2018
URL http://www.ece2018.com/scientific-programme/day-1-monday-2-july/
 
Description Invited Lecture - Antivec Network 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Presentation give on 'The Gnatwork'. 21/06/19.
Year(s) Of Engagement Activity 2019
URL https://www.gla.ac.uk/research/az/antivec/
 
Description Invited Lecture - Public Health Interest Group (London School Hygiene and Tropical Medicine) 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Invited talk presented and discussion session particiation (09/04/19)
Year(s) Of Engagement Activity 2019
 
Description Invited Lecture - Wageningen University and Research 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Talk title: Culicoides-borne arboviruses in Europe - Invited lecture
Year(s) Of Engagement Activity 2019
 
Description Invited Seminar University College London 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Invited seminar at UCL to describe my ongoing research
Year(s) Of Engagement Activity 2018
 
Description Invited lecturer for MSc Biological Electron Microscopy, University of Copenhagen 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact 25 internal students from University of Copenhagen visited the core imaging facility for three days during which time I delivered lectures and interacted with the students during practical workshops. This sparked questions and discussion relating to their individual projects.
Year(s) Of Engagement Activity 2019
 
Description Invited participant, Improving the Use of Evidence in Policymaking in the UK Government (Parliamentary Office of Science and Technology) 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Policymakers/politicians
Results and Impact Invited to attend meeting to launch POST report "Improving the Use of Evidence in Policymaking in the UK Government" as a result of previous collaboration.
Year(s) Of Engagement Activity 2017
URL http://www.sciencecampaign.org.uk/resource/casereportimprovingtheuseofevidence2017.html
 
Description Invited presentation at the Friedrich Loeffler Institute (January; Riems) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Formal presentation on biting midges and bluetongue to professional audience with question session and collaborative meetings
Year(s) Of Engagement Activity 2019
 
Description Invited presentation: 'Beyond Jenner: The Future of Vaccines', Discovery Day, Dr Jenner House Museum and Gardens 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Invited by the British Society for Immunology to give a presentation on vaccine research at the 'Beyond Jenner: The Future of Vaccines', Discovery Day, Dr Jenner House Museum and Gardens, Berkeley, UK.
Year(s) Of Engagement Activity 2018
 
Description Invited seminar at INRAE, France 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact Invited seminar to present our recent and ongoing PRRSV research
Year(s) Of Engagement Activity 2019
 
Description Invited seminar at UC Riverside 05/05/2017 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Invited seminar at University of California, Riverside
Year(s) Of Engagement Activity 2017
 
Description Invited seminar at the National Health Research Institutes, Taiwan. 27 Feb 2018 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact A presentation describing research in my group on mosquito developmental biology and its potential uses for mosquito control; attended by primarily by members of the National Mosquito-Borne Diseases Control Research Center.
Year(s) Of Engagement Activity 2018
 
Description Invited seminar at the University of Nottingham seminar series 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Professional Practitioners
Results and Impact An invited seminar entitled: "Arbovirus encephalitis: from tissue culture, to mosquitoes to mice" was delivered. Discussions for potential collaborations followed.
Year(s) Of Engagement Activity 2017
 
Description Invited seminar in Public Health England- Porton Down 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact Invited seminar entitled: "Innate immune responses and pathogenesis of alphavirus infections". Numerous questions were asked and requests for collaborations were made.
Year(s) Of Engagement Activity 2017
 
Description Invited speaker - Moredun 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Presentation on Bioimaging capabilities at Pirbright to Moredun Institute. Attended by Head of Bioimaging at Roslin too, increased links between these institutes.
Year(s) Of Engagement Activity 2016,2018
 
Description Invited speaker - RMS EM School 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Invited to lecture on certain aspects of electron microscopy. I provided advice on individuals difficulties they had with sample preparation. Delegates had the opportunity to ask me any questions then had relating to any sample type.
Year(s) Of Engagement Activity 2018
 
Description Invited speaker at 9th Neuroinfections Conference, Bialowieza - Poland 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact An invited presentation entitled: "Using small RNAs to fight encephalitic arboviruses" was delivered. The presentation generated a lot of interest and questions.
Year(s) Of Engagement Activity 2017
 
Description Invited speaker at 9th Neuroinfections Conference, Bialowieza - Poland 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact An invited talk entitled: "Using small RNAs to fight encephalitic arboviruses" was delivered. Numerous questions were asked.
Year(s) Of Engagement Activity 2017
 
Description Invited speaker at APHA surveillance meeting 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Invited speaker on insect surveillance at Animal and Plant Health Agency meeting with discussion around current studies.
Year(s) Of Engagement Activity 2018
 
Description Invited speaker at Imperial College 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Professional Practitioners
Results and Impact Gave a talk on our recent research in swine influenza. Discussions with PIs at Imperial after the talk. Established collaborations.
Year(s) Of Engagement Activity 2018
 
Description Invited speaker at South Wilts Virus Group (SWVG) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Professional Practitioners
Results and Impact A talk entitled: "The role of small RNAs in arbovirus infections". Multiple questions were asked and potential for collaborations was identified.
Year(s) Of Engagement Activity 2017
 
Description Invited speaker at a workshop on arboviruses and their vectors in University of Malaya - Malaysia 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact A talk entitled: " The role of small RNAs in arbovirus infections" was delivered. Multiple questions and requests for collaborations followed the presentation.
Year(s) Of Engagement Activity 2017
 
Description Invited speaker at the British VeterinaryPoultry Association 2018 meeting at Boehringer Ingelheim 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact I was invited to present my research findings at the British Veterinary Poultry Association 2018 meeting, held at Boehringer Ingelheim.
Year(s) Of Engagement Activity 2018
 
Description Invited speaker, Merseyside BSI Affinity group 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Professional Practitioners
Results and Impact I described the methods we used to identifyTissue resident memory cells in pigs, which sparked a lively discussion and interest in the technique. As a result I have established two collaborations with colleagues from Liverpool.
Year(s) Of Engagement Activity 2018
 
Description Invited speaker, Surrey University 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Postgraduate students
Results and Impact Under- and postgraduate students attended a talk I gave at the Department of Biological Sciences in Surrey. There was a lively discussion afterwards and as a result I have established a new collaboration.
Year(s) Of Engagement Activity 2018
 
Description Invited speaker, Vienna Veterinary School 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Gave a guest lecture at the Veterinary school in Vienna. There were a lot of questions and discussion after the talk. As a result established collaboration with Gerner Wilhelm's group.
Year(s) Of Engagement Activity 2019
URL https://www.vetmeduni.ac.at/de/graduate-school-pig-and-poultry-medicine/
 
Description Invited talk at Nipah Virus International Conference, Singapore 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Invited presentation on our ongoing work to develop a Nipah virus vaccine for pigs.
Year(s) Of Engagement Activity 2019
 
Description Invited talk at World Vaccine Congress, Washington DC, USA 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Discussed the potential for structural vaccinology to advance livestock vaccine development including examples of FMDV, bRSV and PRRSV
Year(s) Of Engagement Activity 2019
 
Description Invited to talk at the International Conference on Building Human and Animal Health Capacities organised by the Jordan University of Science and Technology 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Participated in the discussion and talked on 'The recent advances in vaccination-based control of poultry diseases'. The need for increased awareness of the pathogen diversity in the design of vaccines and the opportunities from advances in vaccine technologies were discussed
Year(s) Of Engagement Activity 2017
URL http://www.just.edu.jo/conferences/bhahc/Pages/default.aspx
 
Description JN - Woking school 2018 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Careers Fair at a local school encouraging children to consider science as a career and representing the institute in the local community.
Year(s) Of Engagement Activity 2008
 
Description Jennifer Simpson - Big Bang Competition judge 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Schools
Results and Impact The Big Bang Competition recognises and rewards young people's achievements in all areas of science, technology, engineering and maths (STEM), whilst providing them with the opportunity to build their skills and confidence in project-based work. The Competition is open to all UK residents in full-time education or training.
Year(s) Of Engagement Activity 2017,2018
 
Description Jennifer Simpson - Careers Fair at Woking High School 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Pirbright Institute stand at the careers fair. Speak to students interested in a career in science. Sparked questions and discussions.
Year(s) Of Engagement Activity 2018
 
Description Jennifer Simpson - Girls into STEM 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Outreach activity for girls in local schools to demonstrated the variety of careers available within the STEM sector.
Year(s) Of Engagement Activity 2018
 
Description Jennifer Simpson - InFocus article on Bioimaging in high containment 
Form Of Engagement Activity A magazine, newsletter or online publication
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Magazine article describing the facilities for imaging in high containment at Pirbright.
Year(s) Of Engagement Activity 2018
 
Description Keynote speaker - 89th IUVSTA Workshop, Poland 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Keynote seminar to 'high vacuum' community, including life scientists and physical scientists, with experience in microscopy or other high end analystical techniques. increased visibility of the Pirbright Institute.
Year(s) Of Engagement Activity 2019
 
Description Keynote speaker - Danish Virology Society Annual Meeting 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I was personally invited to be keynote speaker at the annual meeting of the Danish Virology Society, to talk about high containment microscopy of cell biology of host-virus interactions. The meeting was held at the State Serum Institute in Copenhagen, followed by a social evening which enabled me to add new contacts into my network. This invitation came direct from the Danish Virology Society, independent of the work I already do with the University of Copenhagen.
Year(s) Of Engagement Activity 2018
 
Description Keynote talk at the World Veterinary Poultry Association meeting 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Invited to give the keynote talk on 'Avian tumour viruses: their contribution to our understanding of cancer' at the World Veterinary Poultry Association XXth Congress in Edinburgh
Year(s) Of Engagement Activity 2017
URL http://www.wvpac2017.com/
 
Description Kingdown School Warminster Wiltshire talk to 6th form students about career in science. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact I presented my career path to the students to engage them on STEM careers. I had a good level of discussion and many questions.
Year(s) Of Engagement Activity 2018
 
Description LEAF open farm Sunday- JH, GM, WM, AH, KR, JS 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact The annual open farm day attended by businesses in the working in the farming industry in the Guilford area
Year(s) Of Engagement Activity 2019
 
Description Laboratory Science Animal association (LASA) meeting in Birmingham, Invited speaker 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Gave a presentation on the tolls we have developed to study swine influenza
Year(s) Of Engagement Activity 2018
URL http://www.lasa.co.uk/meetings/
 
Description Lecture given to University of Surrey MSc Veterinary Microbiology students 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Postgraduate students
Results and Impact A 2-hour teaching lecture given to 10 MSc Veterinary Microbiology students at the University of Surrey on 8th December (Lara Harrup)
Year(s) Of Engagement Activity 2017
 
Description Lecture of dynamics of vector-borne diseases 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Undergraduate students
Results and Impact Lecture on the dynamics of vector-borne diseases as part of Part II Pathology: Dynamics of Infectious Diseases course at Univerity of Cambridge
Year(s) Of Engagement Activity 2008,2009,2010,2011,2012,2013,2014,2015,2016,2017,2018,2019,2020
 
Description Lecture on dynamics of infectious diseases 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Postgraduate students
Results and Impact Lecture as part of MSc in Veterinary Microbiology at University of Surrey
Year(s) Of Engagement Activity 2012,2013,2014,2015,2016,2017
 
Description Lecture on entereic coronaviruses to MSc Students (Surrey) 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Postgraduate students
Results and Impact A lecture was presented on the pathology, diagnosis and detection of enteric coronaviruses, including virus replication and interaction with the host. The students were engaged and asked/answered question throughout the session. We were invited to repeat the lecture for the subsequent student intakes.
Year(s) Of Engagement Activity 2015,2016,2018
 
Description Lorin Adams (Gordon's School Careers Fair) 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Describe the research that lead to improve control systems against infectious viral diseases of animals and from animals to humans.
Year(s) Of Engagement Activity 2019
 
Description MMC Learning Zone - invited speaker 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Microscience Microscopy Conference, speaker at the Learning Zone - specimen preparation for life sciences. Increase the visibility of Pirbright Institute
Year(s) Of Engagement Activity 2019
 
Description MMC2017 Learning Zone seminar 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact Presented a seminar on preparation of samples for electron microscopy to 24 MMC2017 delegates. Questions followed asking for advice on specific examples, also advice on interpretation of data and how to improve results.
Year(s) Of Engagement Activity 2012,2015,2017
 
Description MSc student visit 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Postgraduate students
Results and Impact I hosted several MSc students from University of Surrey, discussed avian research with them and demonstrated some laboratory techniques. The students gained an understanding of academic research and laboratory work.
Year(s) Of Engagement Activity 2018,2019
 
Description Malaysia workshop 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Organised and participated in workshop on genetic control of mosquitoes, primarily focusing on refractory methods. Included a set of open lectures from international speakers at University of Malaysia and more focus workshop-style meetings. Several reported changes to research activities and also opinions, also follow-up meeting organized in Glasgow later in 2017
Year(s) Of Engagement Activity 2017
 
Description Mapping antigenic determinants of H9N2 avian influenza viruses and improving vaccines and diagnostics" presented at Foreign Talent Lecture: New strategies and new techniques for the prevention and control of major avian diseases. Shandong Binzhou Animal Science & Veterinary Medicine Academy, Binzhou, China. 6th August 2018. 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact The focus of the meeting was to improve the control systems against poultry diseases including development of improved vaccines and diagnostics
Year(s) Of Engagement Activity 2018
 
Description Medical and Veterinary Virus Research Symposium (MVVR) Kenya 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Talk at international conference on One Health research in Kenya
Year(s) Of Engagement Activity 2017
 
Description Microbiology Society Annual conference (UK) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact Poster or oral presentation at Microbiology Society annual conference, a major international event. The participants included researchers from many different areas of science. This provides a platform to promote research to a wider audience as well as discuss the key findings with experts in my own field.
Year(s) Of Engagement Activity 2010,2011,2012,2013,2014,2015,2016,2017,2018,2019
 
Description Microbiology Society Avian Focus Meeting (UK) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact I gave a 15 minute oral presentation to an audience of around 80 people in 2016 and 2018. The audience consisted of researchers from many different scientific institutions from different areas of avian research. This was a great opportunity to present to experts in this field and gain novel insights into the project and the possible applications of my work.
Year(s) Of Engagement Activity 2016,2018
 
Description Microbiology Today article 
Form Of Engagement Activity A magazine, newsletter or online publication
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Invited article titled 'How to Image an Exotic Virus' in special Imaging issue (February 2018).
Year(s) Of Engagement Activity 2018
 
Description Microsoc 2018 "Structure-guided identification of a pathogen with pandemic potential" poster 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Presented a poster on our research at the MicroSoc annual meeting
Year(s) Of Engagement Activity 2018
 
Description Mock Interview Programme Cove School- Ai, AA 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Stimulating increased interest in science and research
Year(s) Of Engagement Activity 2019
 
Description Molecular Determinants for Antigenicity and Vaccine Efficacy of H9 and H7 Avian Influenza Viruses. Newton Agham Researcher Links Workshop: Novel vaccines and diagnostics technologies against Emerging and re-emerging veterinary Pathogens. 4-7 February 2019 at Rizal Pak Hotel, Manila, Philippines. 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact The talk and the discussion on the improvement of disease control systems (vaccines and diagnostics) to prevent avian influenza viruses infection in poultry and zoonotic infections in humans.
Year(s) Of Engagement Activity 2019
 
Description Molecular determinants modulating avian influenza H7N9 virus antigenicity. Presented at The Microbiology Society Annual Conference 2019, 8-11 April ICC Belfast, UK. 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact The talk and the discussion on the improvement of disease control systems (vaccines and diagnostics) to prevent avian influenza viruses infection in poultry and zoonotic infections in humans.
Year(s) Of Engagement Activity 2019
 
Description Molecular determinants of antigenicity of H7 and H9 avian Influenza viruses. Presented as invited speaker at BBSRC UK-India Partnering Award seminar: University of Liverpool. 2 May 2019. 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact The talk and the discussion on the improvement of disease control systems (vaccines and diagnostics) to prevent avian influenza viruses infection in poultry and zoonotic infections in humans.
Year(s) Of Engagement Activity 2019
 
Description Munir Iqbal: Cheltenham Science Festival 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Describe the research that lead to improve control systems against infectious viral diseases of animals and from animals to humans.
Year(s) Of Engagement Activity 2019
 
Description NA PRRS Symposium & Conference of Research Workers in Animal Disease - Oral and poster presentations 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Presentation title: Identification of host proteins that interact with non-structural proteins-1a and -1ß of PRRSV-1
Year(s) Of Engagement Activity 2019
 
Description Negative Strand Virus 2018 Meeting "Structure-guided identification of morbilliviruses with zoonotic potential" Poster 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme?