Low Containment

Lead Research Organisation: The Pirbright Institute
Department Name: UNLISTED

Abstract

Low containment experimental facilities support, enhance and extend the range of viruses studied at the Pirbright Institute. The primary infrastructure associated with these facilities is the BBSRC National Centre for Vaccinology, the Jenner Building, which was opened in 2016. This facility houses a wide range of groups working on strategically important endemic and exotic viral pathogens that can be handled under low containment, including Marek’s disease virus, infectious bronchitis virus and low pathogenicity animal influenzas. In addition, the Jenner building also houses groups that study the genetics and genomics of virus hosts, using state of the art technologies. This capability is nationally unique both because it supports studies of viruses not replicated elsewhere in the UK and because of its position in close proximity to High Containment Experimental Facilities in Pirbright.
Our in vivo facilities supporting avian research are housed in a temporary facility. This enables study of internationally important avian viruses within the natural host. During the term of the CCG, new facilities will be constructed on the Pirbright site, enabling expansion of this work. This includes a specified pathogen free (SPF) hatchery and rearing facility due for delivery in early 2018 and refurbishment of current high containment animal facilities to deliver a low containment Poultry Experimental Facility in late 2018.

The Low Containment National Capability has been used to address COVID-19 related challenges from 1 April 2020.

Organisations

Publications

10 25 50
 
Description The key development within low containment science at The Pirbright Institute is that of providing facilities that allow handling of non-infectious and low pathogenicity agents within laboratories and a range of desk based activities including bioinformatics and epidemiological modelling. The facilities are required to integrate with high-containment buildings on site and also provide areas for analyses of data from experiments and are expected to be safe for the user and environment and experience minimal interruptions to work through infrastructure works. These objectives have been achieved in the following ways: 1) Interruptions to science are within the boundaries set for the facilities. The BBSRC National Vaccinology Centre: The Jenner Building is part of the planned preventative maintenance schedule and is now at capacity for science, supporting low containment studies and the majority of Avian researchers. 2) The Houghton poultry facility (Specified Pathogen Free hatchery) and Pirbright remains a hub for Avian research as demonstrated by the production of publications, outreach activities and interactions with industry. These will be complemented by high-containment space for avian studies in the Biggs facility which is due to open in 2022. 3) Relationships with the National Avian Research Facility remain open and were reinforced by meetings that included BBSRC (UKRI) members. 4) Bioinformatics capabilities have been strengthened significantly with expansion and upgrade of computing facilities and staff, the latter provisioned from the Science Services CCG component. 5) Significant progress has been made in opening up the low containment facilities for training and also in provision of outreach activities. 6) Insectary facilities, which have seen a significant rise in demand in the reporting period within the science conducted by Pirbright, have been expanded via refurbishment of the IS4L facility followed by three major successful bids for refurbishment in 2019, 2020 and 2021. This is a medium term, interim solution. Reliability of the Philip Mellor Insectary has improved substantially in the reporting period due to planned preventative maintenance and more recently a major shutdown refurbishment in 2021. In total >170 peer-reviewed publications and >430 engagement activities have been enabled by this infrastructure, including a major focus on Avian research and viruses spread by arthropod vectors.
Exploitation Route By acting as a hub for fundamental avian viral research, Pirbright provides disease information and expertise for a variety of high-consequence endemic viruses including Marek's disease virus. Through the use of scientific networks these areas are both extended and enhanced by external researchers, in addition to being provided to policy makers for outbreak scenario planning. In addition, a significant proportion of studies are aimed at modifying or creating vaccines that are used in the poultry sector which entails successful translation to use in industry. Bioinformatic outputs in open access databases and journals are available to workers worldwide and are a valuable resource for comparative studies of both phylogenetics and immunology. Facilities supporting insect rearing and analysis are largely focused on mosquitoes and biting midges which are supplied to both internal and external users. Studies using genetic modification of mosquitoes are part of a vanguard of research using emerging technologies that may be applicable across multiple hosts and scenarios. Hence technologies developed at Pirbright may inform global researchers in this subject area.
Sectors Agriculture, Food and Drink

 
Description The findings of research on both avian and arthropod interactions with viruses have an impact on fundamental science and underpin global research in these subject areas. Within the avian research, there is also a significant use in global policy decision making regarding viruses of Poultry with contributions to both working groups on disease control and the production of vaccines. Bioinformatic studies conducted form part of global databases that enable comparative studies of hosts and vectors carried out by researchers worldwide and can additionally information regarding the origin and spread of virus outbreaks in the case of avian studies.
First Year Of Impact 2017
Sector Agriculture, Food and Drink
Impact Types Societal,Economic,Policy & public services

 
Description BBSRC 18ALERT panel member
Geographic Reach National 
Policy Influence Type Participation in a advisory committee
Impact Provision of multi-user equipment to core facilities to enable BBSRC remit science. Part of the remit for this call was to allow shared access from other institutions, widening the impact of BBSRC investment.
 
Description BBSRC ALERT19 panel member (invited)
Geographic Reach National 
Policy Influence Type Participation in a advisory committee
Impact Panel for ALERT18 multi-user equipment award by BBSRC
 
Description BBSRC-Newton Fund Agricultural Data Re-use Advisory Group
Geographic Reach National 
Policy Influence Type Participation in a advisory committee
Impact Advice on re-usability of data generated in research projects.
 
Description Bioimaging Steering Group
Geographic Reach Local/Municipal/Regional 
Policy Influence Type Membership of a guideline committee
Impact Better management of workload and facility internally
 
Description Coalition for Epidemic Preparedness Innovations Nipah Taskforce Member
Geographic Reach Multiple continents/international 
Policy Influence Type Participation in a advisory committee
 
Description Contributed to Chief Medical Officers report- Genomics section
Geographic Reach Europe 
Policy Influence Type Implementation circular/rapid advice/letter to e.g. Ministry of Health
URL https://assets.publishing.service.gov.uk/government/uploads/system/uploads/attachment_data/file/6310...
 
Description Contribution to a POSTnote on 'Reducing UK Antibiotic Use in Animals'
Geographic Reach National 
Policy Influence Type Participation in a advisory committee
 
Description Data on SARS-COV-2 antigenicity for government committees, e.g. UKHSA variant technical group, NERVtag
Geographic Reach National 
Policy Influence Type Implementation circular/rapid advice/letter to e.g. Ministry of Health
Impact This information was routinely fed into UKHSA and government decision making on managing the Covid-19 pandemic
 
Description EFSA GMO Gene Drive WG Hearing Expert
Geographic Reach Europe 
Policy Influence Type Participation in a advisory committee
Impact Hearing Expert for EFSA GMO Gene Drive Working Group. Investigated environmental risk assessment of gene drive organisms relative to "conventional" GMOs. Led to report on subject https://www.efsa.europa.eu/en/news/efsa-advises-risk-assessment-engineered-gene-drives
URL https://www.efsa.europa.eu/en/news/efsa-advises-risk-assessment-engineered-gene-drives
 
Description Grants Review Team
Geographic Reach Local/Municipal/Regional 
Policy Influence Type Membership of a guideline committee
Impact More effective inclusion of microscopy technology in grants submitted from the Institute
 
Description Human Infection Challenge Models for Vaccination
Geographic Reach Europe 
Policy Influence Type Influenced training of practitioners or researchers
 
Description IUCN Assessment of Synthetic Biology and Biodiversity Conservation
Geographic Reach Multiple continents/international 
Policy Influence Type Participation in a advisory committee
URL https://www.iucn.org/theme/science-and-economics/our-work/other-work/synthetic-biology-and-biodivers...
 
Description IUCN Technical Subgroup on Synthetic Biology and Biodiversity Conservation
Geographic Reach Multiple continents/international 
Policy Influence Type Participation in a advisory committee
 
Description Inter-institute imaging network
Geographic Reach National 
Policy Influence Type Participation in a advisory committee
 
Description Invited member of RCaH Science Advisory Board
Geographic Reach National 
Policy Influence Type Participation in a advisory committee
Impact The Research Complex at Harwell is a UKRI funded building with the remit of enabling researchers across many disciplines to take advantage of the national facilities on the Harwell campus. RCaH is going through significant change at the moment and the advisory board has been asked to contribute to defining its future direction.
 
Description MSc research training
Geographic Reach Multiple continents/international 
Policy Influence Type Influenced training of practitioners or researchers
Impact The immediate outcome of this impact activity is that these Masters students went on to the following subsequent career destinations: PhD programme: 2 students Technical position in research institution: 1 student
 
Description Member of RMS EM Section committee
Geographic Reach Multiple continents/international 
Policy Influence Type Membership of a guideline committee
Impact This committee informs the RMS on which areas to invest funds in regarding training, conferences, outreach events. RMS members are international.
 
Description Member of the Board of the Jenner Vaccine Foundation
Geographic Reach National 
Policy Influence Type Membership of a guideline committee
Impact Helping with the strategic decisions of the Board of the Jenner Vaccine Foundation
 
Description Member of the Virus Division for the Microbiology Society
Geographic Reach National 
Policy Influence Type Membership of a guideline committee
 
Description Microbiology Society Members Panel
Geographic Reach Multiple continents/international 
Policy Influence Type Participation in a advisory committee
URL https://microbiologysociety.org/why-microbiology-matters/council-governance/standing-panels/members-...
 
Description Microbiology Society Policy Committee
Geographic Reach Europe 
Policy Influence Type Participation in a advisory committee
 
Description Netherlands Commission on Genetic Modification (COGEM) Project "Gene drives, inventory of previous research and experience"
Geographic Reach Europe 
Policy Influence Type Gave evidence to a government review
 
Description POSTnote Report on Climate and vector-diseases in humans in the UK
Geographic Reach National 
Policy Influence Type Gave evidence to a government review
 
Description PPR Global Research and Expertise Network (PPR GREN) membership (OIE/FAO)
Geographic Reach Multiple continents/international 
Policy Influence Type Participation in a advisory committee
 
Description Publication policy document for core facilities - EM-UK
Geographic Reach Multiple continents/international 
Policy Influence Type Influenced training of practitioners or researchers
Impact I am a founder member of the network created for electron microscopists within UK, EM-UK. The annual meeting is highly productive, and training and managing core facilities is regularly discussed. Recognition of these facilities is essential for their continued existence so we produced a generic document hosted on the RMS website which can be adopted by any core facility in any institution, and describes the publication policy which users had to adhere to. This has already raised the profile of core facilities around the country and is a successful outcome from the EM-UK meetings.
 
Description RCUK Engagement workshop, Cosener's House, 20-21 April 2017
Geographic Reach National 
Policy Influence Type Influenced training of practitioners or researchers
 
Description Royal Microscopical Society Training Focussed Interest Group
Geographic Reach Multiple continents/international 
Policy Influence Type Influenced training of practitioners or researchers
Impact A review of the microscopy training courses available globally has been undertaken. We are in the process of collating and preparing a gap analysis. This will be used to guide RMS policy on training provided in the UK, both UK and international delegates.
 
Description Scientific Advisory Group for Emergencies Exercise (Royal Society: 11/06/19): Examined simulated emergence of arbovirus in the UK with main decision making bodies and personnel.
Geographic Reach National 
Policy Influence Type Gave evidence to a government review
 
Description Training MSc students
Geographic Reach Local/Municipal/Regional 
Policy Influence Type Influenced training of practitioners or researchers
Impact Delivered a new training session to MSc students teaching viral quantification assays. This lead to a greater understanding of laboratory techniques and academic research.
 
Description WHO R&D Blueprint Roadmaps: Consultation on Nipah virus
Geographic Reach Multiple continents/international 
Policy Influence Type Participation in a advisory committee
 
Description A One Health Approach to Alkhumra Haemorrhagic Fever Virus Species Tropism, Pathogenesis and Epidemiology
Amount £99,082 (GBP)
Organisation The Pirbright Institute 
Sector Academic/University
Country United Kingdom
Start 09/2022 
End 04/2026
 
Description A One Health approach to pan-valent morbillivirus vaccines
Amount £773,237 (GBP)
Funding ID BB/R019843/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 04/2018 
End 03/2021
 
Description Active and passive Immunity induced by aerosols
Amount £100,000 (GBP)
Funding ID BB/R506448/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 09/2017 
End 08/2021
 
Description An immunogenetic approach to guide the need for booster shots and combat immune failure in SARS-CoV-2 vaccine response
Amount £987,464 (GBP)
Funding ID MR/W020629/1 
Organisation Medical Research Council (MRC) 
Sector Public
Country United Kingdom
Start 07/2021 
End 08/2022
 
Description Antigenic characterisation of infectious bursal disease virus to improve vaccination strategies and vaccine design
Amount £450,685 (GBP)
Funding ID BB/S014594/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 06/2019 
End 12/2021
 
Description Assessing the functionality of avian BST2 as a viral restriction factor.
Amount £50,000 (GBP)
Organisation University of Oxford 
Sector Academic/University
Country United Kingdom
Start 09/2021 
End 09/2025
 
Description Assessment of the immunogenicity of bovine herpesvirus 4-based vectors delivering Nipah virus glycoproteins in swine
Amount £41,895 (GBP)
Organisation European Commission 
Sector Public
Country European Union (EU)
Start 02/2019 
End 04/2019
 
Description Aviagen Industry Funding
Amount £422,383 (GBP)
Organisation Aviagen Group 
Sector Private
Country United States
Start 08/2019 
End 12/2020
 
Description BBSRC - responsive mode
Amount £187,370 (GBP)
Funding ID BB/S007911/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 03/2019 
End 03/2022
 
Description BBSRC IAA The Pirbright Institute
Amount £300,000 (GBP)
Funding ID BB/S506680/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 03/2018 
End 03/2021
 
Description BBSRC Newton Fund Swine and Poultry Research Initiative
Amount £865,623 (GBP)
Funding ID BB/R01275X/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 03/2018 
End 06/2021
 
Description British Egg Marketing Board PhD studentship
Amount £76,000 (GBP)
Organisation British Egg Marketing Board 
Sector Private
Country United Kingdom
Start 09/2018 
End 09/2022
 
Description Broad and effective protection against influenza achieved by viral vectored vaccines
Amount £1,607,691 (GBP)
Funding ID MR/S037160/1 
Organisation Medical Research Council (MRC) 
Sector Public
Country United Kingdom
Start 04/2019 
End 04/2022
 
Description Commonwealth Scholarship. Molecular determinants impacting avian influenza H9N2 virus evolution, replication fitness and virulence
Amount £200,000 (GBP)
Funding ID LKCS-2019- 665 
Organisation British Council 
Sector Charity/Non Profit
Country United Kingdom
Start 11/2019 
End 10/2022
 
Description Components of the Medea gene drive system in a mosquito Aedes aegypti
Amount £1,209,348 (GBP)
Funding ID BB/S001964/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 11/2018 
End 10/2021
 
Description Coronavirus regulation of cellular antiviral responses and cross species transmission
Amount £52,000 (GBP)
Organisation The Pirbright Institute 
Sector Academic/University
Country United Kingdom
Start 09/2022 
End 09/2026
 
Description DARPA PREEMPT
Amount $2,700,000 (USD)
Organisation Defense Advanced Research Projects Agency (DARPA) 
Sector Public
Country United States
Start 09/2018 
End 03/2022
 
Description Defining the circadian clock in chicken cells and its impact upon viral replication
Amount £4,500 (GBP)
Organisation The Houghton Trust 
Sector Charity/Non Profit
Country United Kingdom
Start 11/2019 
End 11/2020
 
Description Defining the molecular signature of pericyte dysfunction in severe dengue patients
Amount $20,000 (USD)
Organisation UGPN 
Sector Academic/University
Start 09/2020 
End 07/2021
 
Description Dengue haemorrhage: a novel role for perivascular cells
Amount £74,137 (GBP)
Organisation University of Surrey 
Sector Academic/University
Country United Kingdom
Start 09/2021 
End 09/2024
 
Description Development of next generation vaccine technology inducing rapid and strong immunity through targeted delivery of antigens to chicken immune cells
Amount £473,080 (GBP)
Funding ID BB/T013087/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 05/2020 
End 05/2023
 
Description Development of therapeutic monoclonal antibodies for coronaviruses
Amount £120,000 (GBP)
Organisation The Pirbright Institute 
Sector Academic/University
Country United Kingdom
Start 09/2022 
End 09/2026
 
Description Dissecting the molecular pathways of MDV oncoprotein Meq for understanding pathogenesis and aid vaccine development
Amount £440,000 (GBP)
Funding ID BB/R007896/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 03/2018 
End 03/2021
 
Description Enhancing protective efficacy of avian influenza vaccines through targeted delivery of protective antigens to chicken immune cells
Amount £98,212 (GBP)
Funding ID BB/R50595X/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 08/2017 
End 09/2021
 
Description FLI- TPI joint PhD studentships Avian influenza immunology markers.
Amount £100,000 (GBP)
Organisation Friedrich Loeffler Institute 
Sector Academic/University
Country Germany
Start 06/2018 
End 06/2022
 
Description Further development of infectious bronchitis virus vaccines adapted for production in cell culture
Amount £262,701 (GBP)
Funding ID BB/R019576/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 09/2018 
End 03/2020
 
Description G2P-UK; A National Virology Consortium to address phenotypic consequences of SARSCoV-2 genomic variation
Amount £2,497,230 (GBP)
Funding ID MR/W005611/1 
Organisation Medical Research Council (MRC) 
Sector Public
Country United Kingdom
Start 02/2021 
End 07/2022
 
Description Genomia Project Grant
Amount £174,052 (GBP)
Organisation Genomia fund 
Sector Charity/Non Profit
Country United Kingdom
Start 02/2019 
End 01/2020
 
Description Global characterisation of interlinked mosquito immune networks
Amount £9,000 (GBP)
Organisation The Pirbright Institute 
Sector Academic/University
Country United Kingdom
Start 07/2021 
End 03/2022
 
Description How does the lung protect itself against influenza?
Amount £120,000 (GBP)
Organisation The Pirbright Institute 
Sector Academic/University
Country United Kingdom
Start 06/2022 
End 07/2026
 
Description Identification and evaluation of swine mAbs in pig influenza challenge model
Amount $599,944 (USD)
Funding ID OPP1201470 
Organisation Bill and Melinda Gates Foundation 
Sector Charity/Non Profit
Country United States
Start 09/2018 
End 10/2019
 
Description Identification of interaction sites between the genomic segments of influenza virus as putative novel anti-viral targets
Amount £100,000 (GBP)
Organisation The Pirbright Institute 
Sector Academic/University
Country United Kingdom
Start 09/2019 
End 09/2024
 
Description In vitro evaluation of peptide-based immune checkpoint inhibitors as potential molecular adjuvants for improved PRRS vaccines
Amount £4,921 (GBP)
Organisation The Pirbright Institute 
Sector Academic/University
Country United Kingdom
Start 07/2019 
End 03/2020
 
Description Internal Seed Fund- Development of an IBDV diagnostic test that can differentiate infected from vaccinated animals, for use in surveillance.
Amount £10,000 (GBP)
Organisation The Pirbright Institute 
Sector Academic/University
Country United Kingdom
Start 12/2019 
End 02/2020
 
Description LVIF
Amount $1,500,000 (CAD)
Organisation Bill and Melinda Gates Foundation 
Sector Charity/Non Profit
Country United States
Start 03/2018 
End 09/2020
 
Description NC3Rs Research Project Grant
Amount £436,339 (GBP)
Funding ID NC/R001138/1 
Organisation National Centre for the Replacement, Refinement and Reduction of Animals in Research (NC3Rs) 
Sector Charity/Non Profit
Country United Kingdom
Start 12/2017 
End 11/2019
 
Description Newton Fund: UK-China-Philippines-Thailand Swine and Poultry Research Initiative
Amount £841,995 (GBP)
Funding ID BB/R012679/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 01/2018 
End 12/2020
 
Description PRCV model to better understand immunity to SARS-CoV2
Amount £50,000 (GBP)
Organisation The Pirbright Institute 
Sector Academic/University
Country United Kingdom
Start 08/2021 
End 03/2022
 
Description Pathfinder BB/R012431/1 Grant holder: Dr Mark Fife. Grant title: chIFITM knockdown/knockout technology as a platform technology for increased vaccine yields in SPF Eggs.
Amount £12,500 (GBP)
Funding ID BB/R012431/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 11/2017 
End 02/2018
 
Description Pirbright Institute Flexible Talent Mobility Account
Amount £180,000 (GBP)
Funding ID BB/S507945/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 11/2018 
End 03/2021
 
Description Protecting poultry from avian influenza, Newcastle disease, infectious bronchitis, and Gumboro disease with a single dose of a multivalent vaccine
Amount £448,937 (GBP)
Funding ID BB/W003325/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 01/2022 
End 12/2023
 
Description Recombinant pseudorabies virus as a multivalent vectored vaccine platform for emerging and endemic porcine diseases
Amount £80,000 (GBP)
Organisation The Pirbright Institute 
Sector Academic/University
Country United Kingdom
Start 09/2020 
End 09/2024
 
Description Reducing the Economic and Zoonotic Impact of Avian Influenza (REZIAI): delivering novel vaccines and diagnostics from laboratory to the field.
Amount £400,000 (GBP)
Funding ID BB/S013792/1 
Organisation The Pirbright Institute 
Sector Academic/University
Country United Kingdom
Start 02/2019 
End 01/2021
 
Description SOMAmer technology to diagnose coronavirus infection: veterinary and zoonotic implications
Amount £1 (GBP)
Organisation University of Oxford 
Sector Academic/University
Country United Kingdom
Start 09/2021 
End 09/2025
 
Description Seed Catalyst Award
Amount £25,527 (GBP)
Funding ID ISCF-TFPSA-Pirbright 
Organisation The Pirbright Institute 
Sector Academic/University
Country United Kingdom
Start 11/2018 
End 02/2019
 
Description Taiwan Partnering Award
Amount £40,700 (GBP)
Funding ID BB/S020624/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start  
 
Description The Global Challenges Research Fund
Amount £640,473 (GBP)
Organisation Research Councils UK (RCUK) 
Sector Public
Country United Kingdom
Start 03/2019 
End 03/2024
 
Description The Pirbright Livestock Antibody Hub
Amount $5,500,000 (USD)
Organisation Bill and Melinda Gates Foundation 
Sector Charity/Non Profit
Country United States
Start 11/2019 
End 11/2024
 
Description The genetic and functional analysis of variation in innate immune genes associated with restriction of influenza virus for chicken lines
Amount £422,383 (GBP)
Organisation Aviagen Group 
Sector Private
Country United States
Start 01/2020 
End 03/2021
 
Description The impact of natural genetic variation on ruminant immune responses
Amount £80,000 (GBP)
Organisation University of Southampton 
Sector Academic/University
Country United Kingdom
Start 09/2018 
End 09/2022
 
Description Towards a novel multivalent poultry vaccine: Development of a recombinant infectious bursal disease vectored vaccine encoding conserved B- and T- cell epitopes from infectious bronchitis virus
Amount £5,000 (GBP)
Organisation The Houghton Trust 
Sector Charity/Non Profit
Country United Kingdom
Start 11/2019 
End 11/2020
 
Description UK-China partnership on Global Food Security: Combating avian tumor diseases for sustainable poultry proudction
Amount £382,000 (GBP)
Funding ID BB/R007632/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 03/2018 
End 03/2020
 
Description Understanding the importance of the PI3K pathway in modulating influenza virus replication in chickens and ducks
Amount £100,000 (GBP)
Organisation The Pirbright Institute 
Sector Academic/University
Country United Kingdom
Start 09/2021 
End 04/2025
 
Description [YY-EEID US-UK XXXX] Evaluating how immunosuppression influences influenza A virus transmission and evolution in wild and domestic birds
Amount £606,360 (GBP)
Funding ID BB/T008806/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 05/2019 
End 06/2023
 
Description iCASE studentship: Production and assessment of antiviral prophylactic properties of natural biomolecules against avian influenza and Newcastle disease viruses affecting poultry
Amount £100,000 (GBP)
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 09/2019 
End 09/2023
 
Title A ligation and restriction enzyme independent cloning technique: an alternative to conventional methods for cloning hard-to-clone gene segments in the influenza reverse genetics system 
Description we developed an easy and efficient ligation and restriction enzyme independent (LREI) cloning method for cloning influenza gene segments into pHW2000 vector. The method involves amplification of megaprimers followed by PCR amplification of megaprimers using a bait plasmid, DpnI digestion and transformation. ligation and restriction enzyme independent (LREI) cloning method represents an alternative strategy for cloning influenza gene segments that have internal restriction sites for the enzymes used in reverse genetics. Further, the problem of genetic instability in bacteria can be alleviated by growing recombinant bacterial cultures at a lower temperature. This technique can be applied to clone any influenza gene segment using universal primers, which would help in the rapid generation of influenza viruses and facilitate influenza research and vaccine development. 
Type Of Material Technology assay or reagent 
Year Produced 2020 
Provided To Others? Yes  
Impact The method will help in the rapid generation of reverse genetic base influenza viruses and facilitate influenza research and vaccine development. 
URL https://virologyj.biomedcentral.com/articles/10.1186/s12985-020-01358-2
 
Title A male-specific cell line from a mosquito Anopheles gambiae 
Description A new male-specific cell line from a mosquito Anopheles gambiae has been isolated from sexed neonate larvae. To our knowledge this is the only cell line derived exclusively from male mosquitoes. 
Type Of Material Cell line 
Year Produced 2019 
Provided To Others? No  
Impact The cell line will greatly facilitate studies on components of the sex determination pathway and of dosage compensation machinery in mosquitoes. There are only several cell lines derived from Anopheles gambiae available and these are female-like. Over-expression of genes involved in male sex determination or dosage compensation is detrimental to female cells, hence use of male-derived cells is necessary for such studies. The cell line will become available upon acceptance of a manuscript describing characterization of that cell line. 
 
Title An ex vivo IBDV infection model using a chicken primary B cell culture system 
Description Recently, it has become possible to culture chicken primary B cells in vitro in the presence of a soluble construct of chicken CD40L that was made at The Pirbright Institute. We have demonstrated that these chicken primary B cells can be infected with infectious bursal disease virus (IBDV). This system will be validated and expanded to other B cell tropic viruses in order to replace the use of infected chickens in research, in an NC3Rs funded grant. 
Type Of Material Model of mechanisms or symptoms - in vitro 
Year Produced 2017 
Provided To Others? Yes  
Impact Using this system, we have determined that chicken primary B cells respond to IBDV infection by inducing Type I IFN responses. However, the induction is more pronounced in cells infected with an attenuated strain compared to a very virulent strain. This is consistent with the very virulent strain down-regulating antiviral responses to a greater extent than other strains which may, in part, explain its enhanced virulence. We also observed a reduction in the expression of key genes involved in B cell proliferation and activation following IBDV infection which was only possible by using the primary B cell culture system. 
URL https://www.ncbi.nlm.nih.gov/pubmed/?term=broadbent+dulwich
 
Title Assistance in establishment of NIBSC WHO International Standard for SARS-CoV-2 
Description We assisted NIBSC and the WHO in developing an international serum standard (for ELISAs, VNTs etc.) for SARS-CoV-2. This is an essential tool for standardising assay results across different laboratories and the reagents have now been used by 100s of labs. The emergence of variants with altered antigencity meant that a new reagent was needed and in early 2022 we also helped with this next generation of reagents. 
Type Of Material Technology assay or reagent 
Year Produced 2021 
Provided To Others? Yes  
Impact Development of an international standard for SARS2 is pivotal for standardising results across various labs working on the pandemic response to Covid. As such this is an essential tool for the international research community. 
 
Title CRISPR/Cas9-based gene editing of avian herpesvirus vectors to develop novel recombinant vaccines 
Description Development of recombinant herpesvirus vaccines are usually achieved by classical homologous recombination methods. The advent of new genome editing tools has enabled its application on avian herpesvirus vaccine strains to develop new recombinant vaccines potentially capable of expressing protective antigens from multiple pathogens 
Type Of Material Technology assay or reagent 
Year Produced 2017 
Provided To Others? Yes  
Impact The major impact will be in the generation of novel vaccines that can simultaneously protect against multiple avian diseases 
URL http://www.poultryworld.net/Health/Articles/2016/12/Is-GM-technology-the-future-of-poultry-vaccines-...
 
Title Cattle MHC genotyping 
Description Using the sequence data generated through the targeted pull down of MHC, we developed a full gene and more targeted PCR approach to genotype cattle for the MHC class I region. This has been applied to many hundreds of samples to enable us to select individuals for breeding as well as survey genetic diversity in beef and diary herds. 
Type Of Material Technology assay or reagent 
Year Produced 2018 
Provided To Others? No  
Impact After publication which we anticipate in 2019, we will appy this method to targeted herds and are already attracting industry interest. 
 
Title Comprehensive analysis of genetic variants across immune loci in indigenous breeds. 
Description The interferon-induced transmembrane (IFITM) protein family comprises a class of restriction factors widely characterised in humans for their potent antiviral activity. Their biological activity is well documented in several animal species, but their genetic variation and biological mechanism is less well understood, particularly in avian species. Here we report the complete sequence of the domestic chicken Gallus gallus IFITM locus from a wide variety of chicken breeds to examine the detailed pattern of genetic variation of the locus on chromosome 5, including the flanking genes ATHL1 and B4GALNT4. We have generated chIFITM sequences from commercial breeds (supermarket-derived chicken breasts), ancient bone samples, indigenous chickens from Nigeria (Nsukka) and Ethiopia, European breeds and inbred chicken lines from The Pirbright Institute, totalling of 211 chickens. Through mapping of genetic variants to the latest chIFITM consensus sequence our data reveal that the chIFITM locus does not show structural variation in the locus across the populations analysed, despite spanning diverse breeds from different geographic locations. However, single nucleotide variants (SNVs) in functionally important regions of the proteins within certain groups of chickens were detected, in particular the European breeds and indigenous birds from Ethiopia and Nigeria. In addition, we also found that two out of four SNVs located in the chIFITM1 (Ser36 and Arg77) and chIFITM3 (Val103) proteins were simultaneously under positive selection. Together these data suggest that IFITM genetic variation may contribute to the capacities of different chicken populations to resist viral infection. 
Type Of Material Model of mechanisms or symptoms - non-mammalian in vivo 
Year Produced 2019 
Provided To Others? Yes  
Impact This paper is currently in press in. BMC Genomics. 
 
Title Correlative Light and Electron Microscopy (CLEM) on IBDV-infected cells 
Description Jenny Simpson and Pippa Hawes have developed the technique of Correlative Light and Electron Microscopy (CLEM). This technique has been used to visualise Virus Factories of IBDV. 
Type Of Material Technology assay or reagent 
Year Produced 2019 
Provided To Others? No  
Impact The Virus Factories (VFs) of IBDV were thought to be paracrystalline arrays of virions in the cytoplasm. However, combining our GFP11-tagged IBDV with CLEM technology revealed that the VFs are actually proteinaceous accumulations in the cytoplasm. This has opened up a new avenue of research in terms of the ultrastructure, formation and function of the VFs. 
 
Title Detection of HA antigens of avian influenza viruses 
Description Assay for detection of recombinant HVT expressing HA antigens of avian influenza viruses 
Type Of Material Technology assay or reagent 
Year Produced 2018 
Provided To Others? Yes  
Impact This new method will help to detect recombinant HVT vaccine expressing HA antigen in infected cell. 
 
Title Developed an in vivo infection model to generate highly pathogenic bovine RSV for challenge studies. Developed an accompanying in vitro titration method to calculate yield of virus produced. 
Description Developed an in vivo infection model to generate highly pathogenic bovine RSV for challenge studies. Developed an accompanying in vitro titration method to calculate yield of virus produced. 
Type Of Material Technology assay or reagent 
Provided To Others? No  
Impact Will help use and improve the calf challenge model of bovine RSV for vaccine testing. 
 
Title Developed assays for evaluation of antigen specific memory chicken T cells 
Description An in vitro model for generation of effector cells from memory T cells for chicken T cells were developed 
Type Of Material Technology assay or reagent 
Year Produced 2020 
Provided To Others? No  
Impact This method will enable scientists working on poultry vaccines and immune response to pathogens to assess memory T cell responses in chickens. 
 
Title Development of CLEM techniques for RSV 
Description We have developed the technical expertise to perform correlative light electron microscopy on respiratory syncytial virus infected cells. This allows us to directly compare immunofluorescent images with transmission EM of the same cell. 
Type Of Material Technology assay or reagent 
Year Produced 2019 
Provided To Others? No  
Impact We have recently submitted a paper and grant building on this expertise, as well as forging new collaborations with Diamond light source. 
 
Title Development of SARS-CoV-2 pseudotypes 
Description Method to allow assessment of SARS-CoV-2 entry, which can be used in various experiments including VNTs to assess humoral immune response (in convalescent or vaccinated individuals) 
Type Of Material Technology assay or reagent 
Year Produced 2020 
Provided To Others? Yes  
Impact Application in the establishment of the SARS-CoV-2 international reference standard by NIBSC 
 
Title Development of assay for assessing antigen-specific T cell responses in chickens 
Description An in vitro assay were developed to generate effector memory T cells from memory T cells (cultured ELISPOT assay for chicken T cells) 
Type Of Material Technology assay or reagent 
Year Produced 2020 
Provided To Others? No  
Impact Enables scientists to assess memory T cell responses induced by poultry vaccines 
 
Title Development of assays for quantifying fusion inhibitory antibodies in sera (mFIT assay) 
Description We have developed a micro-fusion inhibition test (mFIT) assay to identify and quantify antibody responses (to vaccines etc.) which are capable of inhibiting viral induced cell-cell fusion. This test has been developed for various negative strand viruses (Nipah and RSV) as well as SARS-CoV-2 
Type Of Material Technology assay or reagent 
Year Produced 2020 
Provided To Others? Yes  
Impact This assay was used to aide development of the NIBSC WHO international standard for SARS-CoV-2 
 
Title Development of chicken NKT cell tetramer 
Description Tetramer for detection and quantification of chicken NKT cells are developed and tested. 
Type Of Material Technology assay or reagent 
Year Produced 2021 
Provided To Others? No  
Impact This will lead to identification of a novel chicken lymphocytes which has major role in the control of infectious diseases and tumour in chickens. 
 
Title Development of high-throughput assay for examining viral mediated cell-cell fusion 
Description We have developed and optimised high-throughput assays (96 well plate format) to examine viral induced cell-cell fusion, using an adapted Bi-FC approach. We are using this to identify inhibitors of paramyxovirus, e.g. measles and RSV, fusion and attachment proteins as well as SARS-CoV-2. This has been integrated with next-generation platforms such as the Incucyte real-time imaging system available at The Pirbright Institute, and the system has been rationalised so that it is entirely inducible. 
Type Of Material Technology assay or reagent 
Year Produced 2018 
Provided To Others? No  
Impact We are using this assay to examine the innate immune restriction of paramyxovirus replication. 
 
Title Development of tetramers to study immune responses in pigs 
Description The pig is a natural and important host of influenza viruses and is physiologically more comparable to humans than other animal models in terms of size, respiratory tract biology and volume. It is also an important vector in the birds to human infection cycle. A major drawback of the current pig model is the inability to analyze antigen-specific CD8+ T-cell responses, which are critical to respiratory immunity. We addressed this knowledge gap using an established in-bred pig model with a high degree of genetic identity between individuals, including the MHC (Swine Leukocyte Antigen (SLA)) locus. We developed a toolset that included long-term in vitro pig T-cell culture and cloning and identification of novel immunodominant influenza-derived T-cell epitopes. We also generated structures of the two SLA class I molecules found in these animals presenting the immunodominant epitopes. These structures allowed definition of the primary anchor points for epitopes in the SLA binding groove and established SLA binding motifs that were used to successfully predict other influenza-derived peptide sequences capable of stimulating T-cells. 
Type Of Material Technology assay or reagent 
Year Produced 2017 
Provided To Others? No  
Impact Peptide-SLA tetramers were constructed and used to track influenza-specific T-cells ex vivo in blood, the lungs and draining lymph nodes. Aerosol immunization with attenuated single cycle influenza viruses (S-FLU) induced large numbers of CD8+ T-cells specific for conserved NP peptides in the respiratory tract. Collectively, these data substantially increase the utility of pigs as an effective model for studying protective local cellular immunity against respiratory pathogens. 
 
Title Established a method to immortalize bovine antigen presenting cells 
Description Established protocols to prepare lentiviruses expressing the proto-oncogenes Bmi1 and hTERT. Established protocols to transduce and generate immortal bovine antigen presenting cells derived from peripheral blood monocytes. 
Type Of Material Cell line 
Provided To Others? No  
Impact This is the first successful attempt at generating bovine immortal monocytic cell lines. This method can also be applied to immortalize B cells. This will result in reducing the number of animals used in experimentation and considerably reduce costs. 
 
Title Established human T cell lines expressing bovine WC1 genes for the analysis of WC1 signal transduction pathways. 
Description Established human Jurkat T cells expressing bovine the bovine gammadelta WC1 co-receptor 
Type Of Material Cell line 
Provided To Others? No  
Impact This cell line will help determine the function of the WC1 co-receptor in gammadelta T cell activation and function. 
 
Title Generated a MHC-defined bovine monophagocytic cell line. 
Description Using the method described above, successfully generated one MHC-defined bovine monophagocytic cell line (A18) which expresses and single classical MHC gene (HD6). 
Type Of Material Cell line 
Provided To Others? No  
Impact Will help reduce the number of animals used in experimentation. Will help resolve antigen presentation pathways in bovine cells and using bovine pathogens. Will help understand T cell activation pathways using MHC matched and mis-matched cells. Will help investigate bovine NK cell activation. 
 
Title Generated monophagocytic reporter cell lines to study the NF-kB signaling pathway 
Description Generated human monophagocytic cells expressing alkaline phosphatase reporter gene under a NF-kB promoter sequence. 
Type Of Material Cell line 
Provided To Others? No  
Impact This cell line is helping determine the role of RSV genes in immune modulation of monophagocytic cells in response to RSV infection. 
 
Title Generation of cell-free virus using feather follicle stem cells 
Description An in vitro model for generation and purification of cell-free virus using feather follicule stem cells 
Type Of Material Technology assay or reagent 
Year Produced 2020 
Provided To Others? No  
Impact Cell lines could be used to generate cell free vaccines against Marek's disease virus 
 
Title IBDV Reverse Genetics System 
Description We have developed a reverse genetics system for IBDV. 
Type Of Material Technology assay or reagent 
Provided To Others? No  
Impact We have used the reverse genetics system to generate recombinant IBDVs and chimeric recombinant IBDVs that contain genes from classical and very virulent strains of IBDV in the background of a cell culture adapted attenuated strain. This will enable us to study the effect of individual virus genes on pathogenicity. 
 
Title Method to evenly load flowcells with multiple samples 
Description Pirbright has an Illumina NextSeq sequencing machine installed in containment. Given the large amount of sequencing reads produced by each run, multiple samples are usually multiplexed on the same flowcell. However, determining the correct concentrations needed to obtain the same amount of reads for each sample is a hard problem, and usually requires a number of expensive and material-consuming kits to be run prior to sequencing. We have developed a method that allows samples to be evenly multiplexed based only on Bioanalyzer data (we routinely run Bioanalyser on the samples anyway as a form of quality control). The method works well, and reliably across a wide range of sample concentrations and sequenced organisms. 
Type Of Material Technology assay or reagent 
Year Produced 2018 
Provided To Others? No  
Impact The method greatly increased the quality of our output, and drastically simplified our workflow by eliminating the external quantification steps that were previously required. 
 
Title Optimisation of chicken kidney cell culture preparation 
Description We have optimised the method of extracting kidneys and preparing chicken kidney (CK) cell cultures from chickens. CK cells are used for in vitro assays to study avian viruses, in particular infectious bronchitis virus. 
Type Of Material Cell line 
Year Produced 2017 
Provided To Others? No  
Impact This has increased the yield of viable cells and the quality of the cell cultures. We can now obtain the number of cells we require using fewer chicken kidneys, therefore requiring fewer chickens to be culled. This is an important development for the 3Rs. We hope to publish this method for others' information. 
 
Title Optimisation of preparation of TOCs from adult chickens 
Description Tracheal organ cultures (TOCs) are prepared from chicken embryos and used to assess growth of avian viruses that are not able to be propagated in cell culture. We have optimised the method of TOCs preparation from 3-week old chickens and are using these to assess viral genetic stability and phenotype in organ cultures that are a similar environment to the site of replication in whole chickens. 
Type Of Material Cell line 
Year Produced 2018 
Provided To Others? No  
Impact We hope that this technique will reduce the number of chickens required for in vivo experiments, in line with the 3Rs, and plan to publish the method in due course. 
 
Title Panel of ACE2 proteins for screening SARS-CoV-2 host range 
Description A biological library of ACE2 expression constructs representing receptors from 22 different human and animal species. 
Type Of Material Technology assay or reagent 
Year Produced 2020 
Provided To Others? Yes  
Impact Establishment of surveillance tools to examine the potential reverse zoonotic spillover of SARS-CoV-2 into anima species 
 
Title Recombinant antibody generation pipeline - WM 
Description Validated a sequencing protocol at Pirbright for mouse hybridomas, cattle heterohybridomas, and porcine B cells. Vector constructed for humans, mouse, cattle, pigs and chicken to allow ligation of commercially generated antibody gene blocks or PCR products generated by degenerate or 5' RACE PCR. These methods allow the following: • Sequencing mAbs • Making Fab fragments from mAbs • Class switching mAbs • Species switching mAbs 
Type Of Material Technology assay or reagent 
Year Produced 2018 
Provided To Others? Yes  
Impact The methods developed have enabled a standardised pipeline that allows cloning of PCR products or synthetic gene constructs for recombinant antibody generation from various species. This uniform approach avoids development of bespoke protocols for each species thus saving time and resources. 
URL http://www.immunologicaltoolbox.co.uk
 
Title Stable chicken cell lines overexpressing chicken IFITM1,2 and 3 
Description In collaboration with Mark Fife, we have produced chicken stable cell lines overexpressing chicken IFITM1,2, 3 and a mutant chicken IFITM3 lacking palmitoylation sites. 
Type Of Material Technology assay or reagent 
Year Produced 2017 
Provided To Others? No  
Impact These cell lines will be used to evaluate the effect of the IFITM proteins on the replication of different viruses, including IBDV. 
 
Title UniMMap- a pipeline for mapping RNAseq data over repetitive immune complexes. 
Description To exploit the abundance of available short read sequencing data we have developed a pipeline that uses mappability to accurately measure transcription over repetitive gene complexes. This methods uses known haplotypes to examine regions of uniqueness, and then the RNAseq data from the individual to train the method to be species or individual specific. This is particularly important over gene complexes that contain genes involved in the immune system, that are often highly similar in sequence but can have profoundly different functions. 
Type Of Material Technology assay or reagent 
Year Produced 2018 
Provided To Others? No  
Impact the immediate impact will be adding fine resolution data to livestock gene expression atlas projects lead by the FAANG consortium. Further impact will be arising as this method is published and we apply it to numerous existing datasets the are publically avaialable as well as generated at Pirbright and by our collaborators. 
 
Title chimeric IBDVs 
Description Andrew Broadbent has engineered chimeric IBDVs expressing individual genes from a very virulent strain in the backbone of an attenuated strain. 
Type Of Material Technology assay or reagent 
Year Produced 2018 
Provided To Others? No  
Impact These chimeric strains can be used to define viral determinants of virulence. 
 
Title chimeric recombinant IBDV with the hypervariable region from field strains 
Description Using our reverse genetics system and B cell culture system, we have engineered a chimeric IBDV with the backbone of a lab-adapted strain, but containing the hyper variable region of the capsid from a very virulent field strain (UK661). 
Type Of Material Technology assay or reagent 
Year Produced 2020 
Provided To Others? No  
Impact This is a proof of concept that it is possible to engineer an IBDV containing the hypervariable region from another strain. We now plan to engineer chimeric viruses with the hypervariable region from strains belonging to different genogroups from different geographical regions and use them to screen the serum from vaccinated birds to deduce the breadth of antibody responses. 
 
Title in vitro model of IBDV antigenic drift in chicken B cells 
Description IBDV vaccines do not induce sterilising immunity, meaning vaccinated birds can become infected with field strains. As for other viruses, it is thought that vaccine-induced immune selection pressure drives the evolution of antigenic drift variants that accumulate amino acid changes in the hypervariable region of the capsid, which may lead to vaccine failures. However, antigenic drift has not been studied in any detail for IBDV, and there is therefore a paucity of information regarding how plastic the capsid is, how quickly mutations arise and become fixed in the virus population, or whether some are more dominant than others. One way of studying this is to vaccinate chickens with sub-protective doses and then challenge them with field strains of IBDV and then sequence the resulting viruses that emerge. To replace the use of birds in these experiments, we have developed an in vitro model of antigenic drift by serially passaging a field strain, F5270, in the immortalised chicken B cell line, DT40, in the presence of sub-neutralising concentrations of vaccine-induced antibodies to better characterise changes in the capsid. 
Type Of Material Technology assay or reagent 
Year Produced 2019 
Provided To Others? No  
Impact In our model, amino acid mutations arose in the hypervariable region of the IBDV capsid at the same sites previously observed in field studies, validating our model. Furthermore, we demonstrated that mutations arose early, by only 5 passages in chicken B cells, which subsequently became fixed by 10 passages. We are now doing next generation sequencing to better characterise the plasticity of the capsid. 
 
Title method for identification of lung Tissue resident memory cells in pigs 
Description Recent overwhelming evidence indicates the importance of local tissue-resident memory T cells (TRM) in protective immunity. Most work on TRM has been performed in mice and the TRM defined as inaccessible to intravenously administered anti-T cell antibody. However there are very few data on TRM in large animals. For the first time we have defined TRM in the pig influenza model. 
Type Of Material Technology assay or reagent 
Year Produced 2018 
Provided To Others? No  
Impact The identification of TRM and their robust protective capacity in site-specific infection has provided a new paradigm by which to assess T cell-mediated responses and an important new target for vaccine design. Since swine are an economically important species, are used as a large animal model for human infection and play a key role in the emergence of novel and potentially zoonotic influenza viruses, the identification of TRM in pigs will allow us to study their role in immunity to swine influenza.and how best to induce them by immunisation. 
 
Title replication competent recombinant split-GFP infectious bursal disease virus 
Description We have generated a replication competent recombinant split-GFP infectious bursal disease virus (IBDV) where a small GFP11 molecule is tagged to the virus polymerase (VP1) to make IBDV-VP1-GFP11. Infected cells that express the GFP1-10 molecule fluoresce green when the GFP11 tag complements the GFP1-10. The IBDV-VP1-GFP11 colocalises with VP3 and dsRNA and therefore is likely to be present at the sites of virus replication. 
Type Of Material Technology assay or reagent 
Year Produced 2017 
Provided To Others? No  
Impact This tool can be used to study IBDV replication complex development in infected cells. 
 
Title tetracysteine-tagged IBDV 
Description Andrew Broadbent has engineered a tetracysteine (TC)-tagged IBDV that can be used in live cell imaging and co-infection studies with the split-GFP IBDV. 
Type Of Material Technology assay or reagent 
Year Produced 2018 
Provided To Others? No  
Impact This tool, combined with the split-GFP IBDV, will enable us to study co-infection, super-infection exclusion, and the intracellular requirements for viral reassortment. 
 
Title Analysis of paramyxovirus replication using Incucyte real-time imager 
Description We have optimised a data analysis pipeline for the Incucyte real-time imager to allow us to track viral replication in a high-throughput fashion (up to 6 x 96-well plates). This is based on simultaneous analysis of phase, as well as green and red fluorescence. 
Type Of Material Data analysis technique 
Year Produced 2018 
Provided To Others? No  
Impact Only preliminary data to date. 
 
Title E-MTAB-5947- Transcription profiling by array of differential gene expression in chicken primary B cells infected ex vivo with attenuated and very virulent strains of infectious bursal disease virus (IBDV) 
Description Microarray data submitted to ArrayExpress from chicken primary B cells infected with a very virulent and attenuated strain of IBDV. 
Type Of Material Database/Collection of data 
Year Produced 2017 
Provided To Others? Yes  
Impact This is the first ever microarray data of chicken primary B cells infected with IBDV and will be of use to other researchers in the area of B cell biology or IBDV virology 
URL https://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-5947/
 
Title FDA drug screening of RSV and SARS-CoV-2 cell-cell fusion 
Description Using inducible cell-cell fusion assays for SARS-CoV-2 and RSV we have screened the FDA approved use drug library for inhibitors of these viral-induced processes 
Type Of Material Data analysis technique 
Year Produced 2022 
Provided To Others? No  
Impact No impact as yet, data still being validated in live virus assays 
 
Title Full length cattle MHC genes 
Description Using our method for full length MHC gene amplification, the largest reference set ever produced from common haplotypes has been sequenced and deposited in the publicly available data IPD-MHC with associated research tools. 
Type Of Material Database/Collection of data 
Year Produced 2020 
Provided To Others? No  
Impact None yet, but it will allow far greater resolution when analysing MHC polymorphism and evolution in cattle populations. 
URL https://www.ebi.ac.uk/ipd/mhc/group/BoLA/
 
Title Technique for analysing interferon stimulated gene inhibition of RSV cell-cell fusion 
Description We have developed the techniques and performed the experiments to examine the effect of over-expressing individual interferon stimulated genes on RSV (bovine and human) replication and cell-cell fusion. This was achieved through integration of library technologies (for the ISGs) and high throughout imaging platforms. 
Type Of Material Data analysis technique 
Year Produced 2021 
Provided To Others? No  
Impact No impact yet as data has still to be published. Manuscript in preparation. 
 
Description Aerosol delivery of vaccines and therapeutics 
Organisation Aerogen
Country Ireland 
Sector Private 
PI Contribution Developed the pig influenza model which is a natural host pathogen system
Collaborator Contribution Provided expertise and equipment for aerosol delivery
Impact Successfully delivered vaccines by aerosol
Start Year 2015
 
Description Andrew Davidson - Proteome of viral replication organelles 
Organisation University of Bristol
Department Avon Longitudinal Study of Parents and Children (ALSPAC)
Country United Kingdom 
Sector Academic/University 
PI Contribution Projects are being initiated to study the cellular proteins associated with coronavirus replication organelle membranes and a funding application has been submitted to BBSRC.
Collaborator Contribution Andrew has provided assistance with experimental planning and drafting of a grant application. He has also provided protocols to allow preliminary data to be generated. Once protocols have been established, Andrew will facilitate mass spectrometry data generation and will analyse the resulting data.
Impact No outcomes to date, projects being initiated and funding applications in progress.
Start Year 2018
 
Description Annika Posautz; University of Veterinary Medicine Vienna. Morbillivirus host-diversity analysis. 
Organisation University of Veterinary Medicine Vienna
Country Austria 
Sector Academic/University 
PI Contribution Sharing of research data regarding morbillivirus host-range and technical support to the project.
Collaborator Contribution Provision of tissue samples from potential morbillivirus hosts, e.g. carnivores.
Impact No outcomes as yet.
Start Year 2018
 
Description Aviagen 
Organisation Aviagen
Country United Kingdom 
Sector Private 
PI Contribution Influenza virus expertise in poultry.
Collaborator Contribution Sharing of commercial poultry breed resources
Impact We have a sponsored PhD studentship to commence Oct 2021 agreed.
Start Year 2020
 
Description BBSRC - Oxford University iCASE Studentship (October 2019- September 2023). Production and assessment of antiviral prophylactic properties of natural biomolecules against avian influenza and Newcastle disease viruses affecting poultry production 
Organisation University of Oxford
Country United Kingdom 
Sector Academic/University 
PI Contribution This collaboration between The Pirbright Institute (UK), Oxford University (UK) and industrial partners in Italy. This project will conceived and developed bu MI. The PhD student (Holly Everest) in my group is investigating the antiviral prophylactic properties of natural biomolecules against avian influenza and Newcastle disease viruses affecting poultry production.
Collaborator Contribution The partners in Italy will produce and purify natural antiviral molecules effective against avian influenza and other poultry viruses.
Impact This project started in January 2020.
Start Year 2020
 
Description BBSRC-UC Davis workshop, 16-18 October 
Organisation University of California, Davis
Department UC Davis College of Biological Sciences
Country United States 
Sector Academic/University 
PI Contribution Presented on "Reproducibility and relevance in vector infection studies". Followed up with discussion of potential publication and funding opportunities.
Collaborator Contribution Various presentations on vectors and vector-borne diseases including, facilities, challenges, opportunities.
Impact Discussion of potential joint publications and grant applications; no tangible impacts yet.
Start Year 2017
 
Description BBSRC-UC Davis workshop, 16-18 October 
Organisation University of Liverpool
Country United Kingdom 
Sector Academic/University 
PI Contribution Presented on "Reproducibility and relevance in vector infection studies". Followed up with discussion of potential publication and funding opportunities.
Collaborator Contribution Various presentations on vectors and vector-borne diseases including, facilities, challenges, opportunities.
Impact Discussion of potential joint publications and grant applications; no tangible impacts yet.
Start Year 2017
 
Description Belsham 
Organisation Technical University of Denmark
Country Denmark 
Sector Academic/University 
PI Contribution Joint PhD studentship and ongoing collaboration on capsid assembly
Collaborator Contribution Joint PhD studentship and ongoing collaboration on capsid assembly
Impact Joint PhD studentship Further funding
Start Year 2016
 
Description Belsham 
Organisation University of Copenhagen
Country Denmark 
Sector Academic/University 
PI Contribution Joint PhD studentship and ongoing collaboration on capsid assembly
Collaborator Contribution Joint PhD studentship and ongoing collaboration on capsid assembly
Impact Joint PhD studentship Further funding
Start Year 2016
 
Description Bioimaging collaboration with Dr. Daniel Clare, Dr. James Gilchrist, Dr. Pippa Hawes - cryo-electron tomography Diamond Light Source 
Organisation Diamond Light Source
Country United Kingdom 
Sector Private 
PI Contribution Development of preliminary data on inclusion body structure to support higher resolution approaches.
Collaborator Contribution Discussion of project and experimental design, technological approaches and limitations.
Impact No outputs to date. Multi-disciplinary team of virologists and structural biologists
Start Year 2020
 
Description Biophysics collaboration with Prof. Stephen McKnight, UTSW-Dallas 
Organisation University of Texas Southwestern Medical Center
Country United States 
Sector Academic/University 
PI Contribution Development of preliminary data on the biophysics and biochemistry of inclusion bodies
Collaborator Contribution Supportive comments on grant applications and experimental design advice.
Impact No outputs to date
Start Year 2020
 
Description Bridget Penman 
Organisation University of Warwick
Department School of Life Sciences
Country United Kingdom 
Sector Academic/University 
PI Contribution We are providing genetic data and diversity measures of MHC and associated receptors to inform the modelling efforts to elucidate which selection pressures are the main drivers for the types of diversity we see in extant species.
Collaborator Contribution They are experts in mathmatical modelling and will provide data to help explain genetic diversity in cattle.
Impact Not yet
Start Year 2017
 
Description CVR 
Organisation University of Glasgow
Department MRC - University of Glasgow Centre for Virus Research
Country United Kingdom 
Sector Academic/University 
PI Contribution Pirbright has several groups investigating various aspects of vector-borne diseases. My group provides mosquito expertise, including gene editing, genetics and transgenesis. CVR have expert arbovirologists with complementary interests. We have developed and exchanged research materials and reagents, shared expertise, and have shared grant funding.
Collaborator Contribution Pirbright has several groups investigating various aspects of vector-borne diseases. My group provides mosquito expertise, including gene editing, genetics and transgenesis. CVR have expert arbovirologists with complementary interests. We have developed and exchanged research materials and reagents, shared expertise, and have shared grant funding.
Impact Joint grant. Several ongoing research strands within this.
Start Year 2021
 
Description Challenge of transgenic chickens with influenza viruses 
Organisation Imperial College London
Department Section of Virology
Country United Kingdom 
Sector Academic/University 
PI Contribution We have challenged transgenic chickens with recombinant avian influenza viruses to determine outcome; infectivity, transmission, pathogenicity, dissemination within host. We also processed the samples generated from these animal experiments at The Pirbright Institute.
Collaborator Contribution Imperial College (Wendy Barclay and Mike Skinner) generated recombinant avian influenza viruses for challenge.
Impact This collaboration has resulted in the successful award of a joint grant with imperial college aiming to carry out challenge experiments on novel transgenic chickens to facilitate control of avian influenza virus in the poultry population. BB/S007911/1 - Investigating the role of ANP32A in the replication of avian Influenza virus
Start Year 2018
 
Description Charles Sande - KEMRI - Kenya: RSV collaboration 
Organisation Kenyan Institute for Medical Research (KEMRI)
Country Kenya 
Sector Public 
PI Contribution Development of research proposal for PhD studentship on the innate immune response during RSV infection. This student will be hosted by my lab for 18 months from 2020 onwards.
Collaborator Contribution Project design. Application for PhD funding and provision of technical support.
Impact The studentship was awarded to Dr. Sande in Kenya and the student has been recruited. They will commence their studies in Spring 2019 and come to the UK in early 2020.
Start Year 2018
 
Description Chicken Microbiome - Robert La Ragione - University of Surrey 
Organisation University of Surrey
Department School of Veterinary Medicine
Country United Kingdom 
Sector Academic/University 
PI Contribution Providing sequencing data of gut microbiome from chickens infected with avian influenza viruses.
Collaborator Contribution Sharing expertise in the analysis of microbiome data
Impact Joint manuscripts and funding opportunities are being explored
Start Year 2019
 
Description Collaboration on SARS-CoV-2 host range with Prof. Wendy Barclay 
Organisation Imperial College London
Country United Kingdom 
Sector Academic/University 
PI Contribution Assaying novel SARS-CoV-2 variant sequences for increased tropism to non-human receptors
Collaborator Contribution Provision of variant Spike constructs
Impact Mutations that adapt SARS-CoV-2 to mink or ferret do not increase fitness in the human airway. Zhou J, Peacock TP, Brown JC, Goldhill DH, Elrefaey AME, Penrice-Randal R, Cowton VM, De Lorenzo G, Furnon W, Harvey WT, Kugathasan R, Frise R, Baillon L, Lassaunière R, Thakur N, Gallo G, Goldswain H, Donovan-Banfield I, Dong X, Randle NP, Sweeney F, Glynn MC, Quantrill JL, McKay PF, Patel AH, Palmarini M, Hiscox JA, Bailey D, Barclay WS. Cell Rep. 2022 Feb 8;38(6):110344. https://pubmed.ncbi.nlm.nih.gov/35093235/
Start Year 2020
 
Description Collaboration on replication of mumps virus in cells 
Organisation University of Copenhagen
Country Denmark 
Sector Academic/University 
PI Contribution Intellectual input so far, training research assistant to prepare samples, imaging samples in transmission electron microscopes
Collaborator Contribution Preliminary confocal microscopy to optimize sample conditions. Preparation of samples for EM.
Impact No outcomes yet
Start Year 2018
 
Description Collaboration on the development of intranasal live-attenuated vaccine against SARS-CoV-2 
Organisation Lancaster University
Country United Kingdom 
Sector Academic/University 
PI Contribution Contributed reagents to tests the immunogenicity and protective efficacy of an intranasal live-attenuated vaccine against SARS-CoV-2 in preclinical animal models including mice and hamster.
Collaborator Contribution The partners undertook most of the work to complete the study detailed in the publication entitled "Immunogenicity and protective efficacy of an intranasal live-attenuated vaccine against SARS-CoV-2 in preclinical animal models". https://www.biorxiv.org/content/10.1101/2021.01.08.425974v1 Abstract of publication. The global deployment of an effective and safe vaccine is currently a public health priority to curtail the coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Here, we evaluated a Newcastle disease virus (NDV)-based intranasal vectored-vaccine in mice and hamsters for its immunogenicity, safety and protective efficacy in challenge studies with SARS-CoV-2. The recombinant (r)NDV-S vaccine expressing spike (S) protein of SARS-CoV-2 administrated via intranasal route in mice induced high levels of SARS-CoV-2-specific neutralizing immunoglobulin A (IgA) and IgG2a antibodies and T cell-mediated immunity. Hamsters vaccinated with two doses of vaccine showed complete protection from clinical disease including lung infection, inflammation, and pathological lesions after SARS-CoV-2 challenge. Importantly, a single or double dose of intranasal rNDV-S vaccine completely blocked SARS-CoV-2 shedding in nasal turbinate and lungs within 4 days of vaccine administration in hamsters. Taken together, intranasal administration of rNDV-S has the potential to control infection at the site of inoculation, which should prevent both the clinical disease and transmission to halt the spread of the COVID-19 pandemic.
Impact The partnership resulted in a joint publication. "Immunogenicity and protective efficacy of an intranasal live-attenuated vaccine against SARS-CoV-2 in preclinical animal models". https://www.biorxiv.org/content/10.1101/2021.01.08.425974v1 Abstract of publication. The global deployment of an effective and safe vaccine is currently a public health priority to curtail the coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Here, we evaluated a Newcastle disease virus (NDV)-based intranasal vectored-vaccine in mice and hamsters for its immunogenicity, safety and protective efficacy in challenge studies with SARS-CoV-2. The recombinant (r)NDV-S vaccine expressing spike (S) protein of SARS-CoV-2 administrated via intranasal route in mice induced high levels of SARS-CoV-2-specific neutralizing immunoglobulin A (IgA) and IgG2a antibodies and T cell-mediated immunity. Hamsters vaccinated with two doses of vaccine showed complete protection from clinical disease including lung infection, inflammation, and pathological lesions after the SARS-CoV-2 challenge. Importantly, a single or double dose of intranasal rNDV-S vaccine completely blocked SARS-CoV-2 shedding in nasal turbinate and lungs within 4 days of vaccine administration in hamsters. Taken together, intranasal administration of rNDV-S has the potential to control infection at the site of inoculation, which should prevent both the clinical disease and transmission to halt the spread of the COVID-19 pandemic.
Start Year 2020
 
Description Collaboration with Dan Horton, Surrey Uni on antigenic cartography 
Organisation University of Surrey
Country United Kingdom 
Sector Academic/University 
PI Contribution Provision of data for analysis, specifically antigenic titres on SARS-CoV-2 neutralisation Provision of data for analysis, specifically data on virus receptor usage
Collaborator Contribution Assessment of antigenic relatedness in neutralisation datasets by antigenic cartography Assessment of receptor usage data by cartography
Impact Funding awarded by UKRI, BBSRC grant BB/W006162/1 Viral entry at the human-animal interface; dissecting the pan-tropic nature of zoonotic viruses.
Start Year 2021
 
Description Collaboration with Keith Chappell on virus neutralisation 
Organisation University of Queensland
Country Australia 
Sector Academic/University 
PI Contribution Assessment of neutralisation to a range of viral immunogens by VNT using pseudotyping technology Assessment of neutralisation to a range of viral immunogens by microfusion inhibition test
Collaborator Contribution Provision of sera from vaccination studies Provision of constructs for pseudotyping and cell-cell fusion
Impact None yet, publications in preparation
Start Year 2020
 
Description Collaboration with Kevin Brown at UKHSA 
Organisation Public Health England
Country United Kingdom 
Sector Public 
PI Contribution Performing neutralisation assays as part of UK Covid response, in particular as part of UKHSA/PHE Consensus Study
Collaborator Contribution Providing sera from Consensus study Advice on project design
Impact Paper under review at Nature Microbiology
Start Year 2021
 
Description Collaboration with Prof. Jane McKeating on RSV and SARS-CoV-2 virology 
Organisation University of Oxford
Country United Kingdom 
Sector Academic/University 
PI Contribution Assessment of SARS-CoV-2 cell-cell fusion under various conditions, and with chemical inhibitors Provision of RSV reagents
Collaborator Contribution Provision of inhibitors and other reagents Experimentation with RSV
Impact None yet, paper under review
Start Year 2020
 
Description Collaboration with Ravi Gupta, University of Cambridge 
Organisation University of Cambridge
Country United Kingdom 
Sector Academic/University 
PI Contribution Collaboration on recurrent emergence of SARS-CoV-2 spike deletion H69/V70 and its role in the biology of this virus. We performed research towards a paper focusing on related sarbecoviruses (RaTG13)
Collaborator Contribution Ravi's group led this research to understand the recurrent emergence of SARS-CoV-2 spike deletion H69/V70, leading to publication in Cell Reports.
Impact Recurrent emergence of SARS-CoV- spike deletion H69/V70 and role in B.1.1.7. Meng B, Kemp SA, Papa G, Datir R, Ferriera I, Marelli S, Harvey WT, Lytras S, Mohamed A, Gallo G, Thakur N, Collier DA, Mlcochova P, The COVID-9 Genomics UK (COG-UK) Consortium, Duncan LM, Carabelli AM, Kenyon JC, Lever AM, De Marco A, Saliba C, Culap K, Cameroni E, Matheson NJ, Piccoli L, Corti D, James LC, Robertson DL, Bailey D, Gupta RK. Cell Rep. 2021 Jun 29;35(13):109292. doi: 10.1016/j.celrep.2021.109292. https://pubmed.ncbi.nlm.nih.gov/34166617/
Start Year 2021
 
Description Collaboration with University of Cambridge 
Organisation University of Cambridge
Country United Kingdom 
Sector Academic/University 
PI Contribution We have performed in vitro experiments with infectious bronchitis virus, contributed to data analysis, preparation of manuscripts and discussed further funding opportunities.
Collaborator Contribution Our partners have analysed viral transcription and translation, and host gene expression after infection of host cells with infectious bronchitis virus, prepared manuscripts and discussed further funding opportunities.
Impact Dinan, A., Keep, S., Bickerton, E., Britton, P., Firth, A. and Brierley, I. (2019) Comparative analysis of gene expression in virulent and attenuated strains of infectious bronchitis virus at sub-codon resolution. J. Virol. 93(18) e00714-19.
Start Year 2012
 
Description Collaboration with University of Copenhagen CFIM on cell biology of virus-host interactions 
Organisation University of Copenhagen
Country Denmark 
Sector Academic/University 
PI Contribution Intellectual input into virus projects which come in to the microscopy core facility at the University. Limited practical work and training given to researchers there. Includes lecturing/organisation of MSc and PhD courses at Copenhagen. PH awarded Visiting Professorship.
Collaborator Contribution Research projects are presented to the core facility, I advise on the best route for achieving the data required and those researchers complete preliminary data following my advice. Collaborators are then able to produce the best possible samples for further advanced microscopy as necessary.
Impact No tangible outcomes yet as we are in early stages. This is multi-disciplinary as projects can come from several University departments (medical, pathology, cell biology, veterinary, food), and other Universities in Denmark (DTU). University awarded Visiting Professorship
Start Year 2018
 
Description Collaboration with University of Oxford 
Organisation University of Oxford
Country United Kingdom 
Sector Academic/University 
PI Contribution We have supplied coronavirus samples and coronavirus expertise to the collaboration.
Collaborator Contribution Our partners have led the research and we have a manuscript currently under review.
Impact Evaluation of imaging technologies to detect coronavirus-infected samples.
Start Year 2020
 
Description Collaboration with Wei Huang, Uni of Oxford on SARS2 neutralisation 
Organisation University of Oxford
Country United Kingdom 
Sector Academic/University 
PI Contribution Hosting of students to undertake SARS2 neutralisation experiments Training and data interpretation
Collaborator Contribution Provision of synthetic antibody-bearing particles for neutralisation experiments
Impact Possible grant application in the future
Start Year 2021
 
Description Collaborative network with Ed Wright, Nigel Temperton and Giada Mattiuzzo on SARS-CoV-2 pseudotyping 
Organisation National Institute for Biological Standards and Control (NIBSC)
Country United Kingdom 
Sector Public 
PI Contribution Development and establishment of SARS-CoV-2 research tools, including pseudotypes and cell-cell fusion assays
Collaborator Contribution Provision of reagents, protocols and technical guidance
Impact We aided in NIBSC's development of WHO international standard for SARS-CoV-2 and have a publication in press on sarbecovirus neutralisation: bioarchived here https://www.biorxiv.org/content/10.1101/2021.08.17.456606v1
Start Year 2020
 
Description Collaborative network with Ed Wright, Nigel Temperton and Giada Mattiuzzo on SARS-CoV-2 pseudotyping 
Organisation University of Kent
Country United Kingdom 
Sector Academic/University 
PI Contribution Development and establishment of SARS-CoV-2 research tools, including pseudotypes and cell-cell fusion assays
Collaborator Contribution Provision of reagents, protocols and technical guidance
Impact We aided in NIBSC's development of WHO international standard for SARS-CoV-2 and have a publication in press on sarbecovirus neutralisation: bioarchived here https://www.biorxiv.org/content/10.1101/2021.08.17.456606v1
Start Year 2020
 
Description Collaborative network with Ed Wright, Nigel Temperton and Giada Mattiuzzo on SARS-CoV-2 pseudotyping 
Organisation University of Sussex
Country United Kingdom 
Sector Academic/University 
PI Contribution Development and establishment of SARS-CoV-2 research tools, including pseudotypes and cell-cell fusion assays
Collaborator Contribution Provision of reagents, protocols and technical guidance
Impact We aided in NIBSC's development of WHO international standard for SARS-CoV-2 and have a publication in press on sarbecovirus neutralisation: bioarchived here https://www.biorxiv.org/content/10.1101/2021.08.17.456606v1
Start Year 2020
 
Description Commonwealth Scholarship. Molecular determinants impacting avian influenza H9N2 virus evolution, replication fitness and virulence 
Organisation Royal Veterinary College (RVC)
Country United Kingdom 
Sector Academic/University 
PI Contribution I develop a research project proposal in which we are investigating how evolutionary changes in the H9N2 avian influenza virus modulate virus fitness and virulence in chickens. This PhD studentship project was funded by Commonwealth scholarship commission for 3 years.
Collaborator Contribution The collaborating Partner Dr Nicola Lewis at Royal Veterinary College is a co-supervisor of the PhD student Thusitha Karunarathna. she provide necessary guidance to achieve prescribed objectives of this project.
Impact The project is at its initial stage.
Start Year 2019
 
Description Components of the Medea gene drive system in a mosquito Aedes aegypti 
Organisation National Health Research Institutes (NHRI) Taiwan
Department Institute of Molecular and Genomic Medicine
Country Taiwan, Province of China 
Sector Charity/Non Profit 
PI Contribution Developed joined research project and lead successful application for funding
Collaborator Contribution Various lines of research, including construction of plasmids and development and characterisation of transgenic mosquito strains; and group discussions about results, conclusions and research progress.
Impact No impact yet.
Start Year 2018
 
Description Components of the Medea gene drive system in a mosquito Aedes aegypti 
Organisation National Institute of Genetics
Department NIG-FLY, Mishima (Drosophila RNAi resources)
Country Japan 
Sector Academic/University 
PI Contribution Developed joined research project and lead successful application for funding
Collaborator Contribution Various lines of research, including construction of plasmids and development and characterisation of transgenic mosquito strains; and group discussions about results, conclusions and research progress.
Impact No impact yet.
Start Year 2018
 
Description Dan Clare - cryo-electron microscopy 
Organisation Diamond Light Source
Country United Kingdom 
Sector Private 
PI Contribution The project aims to characterise proteins complexes associated with coronavirus replication organelles. My team provides significant expertise in replication organelles induced by coronaviruses. Laboratory work is performed by my team. The project is funded by a joint PhD studentship working, predominantly in my laboratory.
Collaborator Contribution Dan provides expertise in cryo-electron microscopy and is advising on experimental design and data analysis as well as providing training.
Impact The student has generated recombinant viruses expressing fluorescently-tagged proteins to allow direct visualisation of replication organelle membranes. These have been validated and cryo-electron microscopy experiments are planned shortly.
Start Year 2020
 
Description David Hughes - University of St. Andrews. RSV preparation. 
Organisation St. Andrews University
Country United States 
Sector Academic/University 
PI Contribution Sharing of preliminary research data on innate immune restriction of RSV.
Collaborator Contribution Provision of cell-lines (Hep2-Npro) to support generation of high titre stocks of RSV. Also associated protocols.
Impact No outcomes as yet, beyond preliminary data.
Start Year 2018
 
Description David Matthews - Proteomics informed by transcriptomics 
Organisation University of Bristol
Department Proteomics Facility
Country United Kingdom 
Sector Academic/University 
PI Contribution Projects are being initiated to study the cellular proteins associated with coronavirus replication organelle membranes and a funding application has been submitted to BBSRC.
Collaborator Contribution David has assisted in project design and drafting of a funding application to BBSRC. We are in the process of generating transcriptomics data, which David will process to provide an expressed protein database for peptide identification following mass spectrometry.
Impact No outcomes to date, a funding application has been submitted to BBSRC.
Start Year 2018
 
Description Dengue microvascular pathogenesis 
Organisation University of Surrey
Department Faculty of Health and Medical Sciences
Country United Kingdom 
Sector Academic/University 
PI Contribution I brought my expertise in dengue and virology, and had significant intellectual input into shaping the research question and methodological approach. I also directly contributed to the training of one shared MSc student, one shared PhD student and three undergraduate students who have contributed to this project so far.
Collaborator Contribution The partner (Dr. Paola Campagnolo, University of Surrey) brought their expertise in cardiovascular biology and had significant intellectual input into shaping the research question and methodological approach. They also directly contributed to the training of one shared MSc student, one shared PhD student and three undergraduate students who have contributed to this project so far.
Impact The collaboration has resulted in the following publications: DOI: 10.1128/mSphere.00258-20 DOI: 10.1128/mSphere.00258-20 This is an interdisciplinary collaboration. The disciplines are microbiology/virology (me) and cardiovascular biology (Dr. Paola Campagnolo, University of Surrey).
Start Year 2018
 
Description Derek Bickhart-USDA 
Organisation U.S. Department of Agriculture USDA
Department Beltsville Agricultural Research Center
Country United States 
Sector Academic/University 
PI Contribution Our group has provided the genomic framework of the cattle NK cell regions and the biological input.
Collaborator Contribution The ARS group has used our data as a framework to map and predict cattle SNPs for variable immune traits using their whole genome data
Impact Research application based on preliminary data to a BBSRC-NIFA call. This collaboration is multi-disciplinary combining molecular biology and computational biology
Start Year 2014
 
Description Development of tetramers in the Babraham pig model 
Organisation Cardiff University
Country United Kingdom 
Sector Academic/University 
PI Contribution Developed the aerosol delivery method of vaccine and provided material (spleen, BAL and blood) from inbred animals (Babraham pigs) following immunisation with the candidate universal vaccine, S-FLU.
Collaborator Contribution Developed porcine influenza specific class I tetramers and provided the epitope map of Babrahams S-Flu responses to NP, M1, M2, PB1 and PB2 that could be presented by SLA-1 or SLA-2 Grew pig T cell clones for the first time in 30 years.
Impact Multidisciplinary - immunology, virology, protein chemistry. Paper not yet published
Start Year 2014
 
Description Distributed Bio - Gates foundation funding - universal influenza vaccine 
Organisation Distributed Bio.
Country United States 
Sector Private 
PI Contribution Pirbright has provided expert advice to the design of adjuvant and challenge studies in appropriate animal models for human influenza vaccine trials. Pirbright is facilitating these studies in line with UK home office requirements and good scientific practice.
Collaborator Contribution Distributed Bio have developed a novel vaccine that is hoped to be a candidate for a human universal influenza virus vaccine.
Impact Funding from Bill and Melinda Gates foundation as part of the Grand challenges - Universal vaccine for influenza for the animal studies involving Pirbright and Distributed Bio
Start Year 2019
 
Description Dr Alex Schock, APHA - Detemining the prevalence and sequences of IBDV in vaccinated chicken flocks in the UK 
Organisation Animal and Plant Health Agency
Country United Kingdom 
Sector Public 
PI Contribution We will determine whether samples are positive for IBDV. If they are positive, we will amplify the hypervariable region of the capsid by PCR and send for Sanger Sequencing
Collaborator Contribution APHA will obtain samples from Veterinary Practices and perform histology.
Impact we have been successful in obtaining funding (Pirbright Internal Seed Award, £10,000)
Start Year 2019
 
Description Dr Carol Cardona, University of Minnesota- avian influenza virus challenge of IBDV or mock-infected chickens 
Organisation University of Minnesota
Country United States 
Sector Academic/University 
PI Contribution We will challenge birds that have been exposed to IBDV, or mock exposed, with highly pathogenic avian influenza (HPAI) strains to determine how IBDV-mediated immunosuppression impacts upon HPAI infection in chickens.
Collaborator Contribution Dr Cardona's group will challenge birds that have been exposed to IBDV, or mock exposed, with low pathogenic avian influenza (LPAI) strains to determine how IBDV-mediated immunosuppression impacts upon LPAI infection in chickens.
Impact we have been successful in obtaining funding (Grant Ref: BB/T008806/1)
Start Year 2019
 
Description Dr Caroline Denesvere 
Organisation French National Institute of Agricultural Research
Department INRA Loire Valley Centre
Country France 
Sector Public 
PI Contribution Worked together to establish a collaboration on development of feather follicular stem cell lines as a platform for the generation of cell-free vaccine against Marek's disease virus.
Collaborator Contribution Provided expertise on cell free Marek's disease and microscopic evaluation of cells generating the virus.
Impact Generation of data leading to a successful BBSRC IPA grant application
Start Year 2020
 
Description Dr David Welchman, APHA -Detemining the prevalence and sequences of IBDV in vaccinated chicken flocks in the UK 
Organisation Animal and Plant Health Agency
Country United Kingdom 
Sector Public 
PI Contribution We wiil determine whether samples are positive for IBDV. If they are positive, we will amplify the hypervariable region of the capsid by PCR and send for Sanger Sequencing
Collaborator Contribution APHA will obtain samples from Veterinary Practices and perform histology.
Impact we have been successful in obtaining funding (Pirbright Internal Seed Award, £10,000)
Start Year 2019
 
Description Dr Finn Grey, The Roslin Institute- Identifying genes essential for the replicaiton of IBDV and IBV in vitro 
Organisation University of Edinburgh
Department The Roslin Institute
Country United Kingdom 
Sector Academic/University 
PI Contribution We will conduct a CRISPR screen and identify genes that are essential for IBDV replication
Collaborator Contribution Finn Grey's group will aid in the CRISPR screen
Impact we have been successful in obtaining a PhD studentship
Start Year 2019
 
Description Dr Helena Maier, The Pirbright Institute - Identifying genes essential for the replicaiton of IBDV and IBV in vitro 
Organisation The Pirbright Institute
Country United Kingdom 
Sector Academic/University 
PI Contribution We will conduct a CRISPR screen and identify genes that are essential for IBDV replication
Collaborator Contribution Helena Maier's group will aid in the CRISPR screen and identify genes that are essential for IBV replication
Impact We have been successful in obtaining a PhD studentship
Start Year 2019
 
Description Dr Holly Shelton, The Pirbright Institute- avian influenza virus challenge of IBDV or mock-infected chickens 
Organisation The Pirbright Institute
Country United Kingdom 
Sector Academic/University 
PI Contribution We will expose birds to IBDV, compared to mock controls
Collaborator Contribution Holly will train my group in conducting studies with avian influenza viruses and, together, we will challenge birds with highly pathogenic avian influenza (HPAI) strains to determine how IBDV-mediated immunosuppression impacts upon HPAI infection in chickens.
Impact we have been successful in obtaining funding (Grant Ref: BB/T008806/1)
Start Year 2019
 
Description Dr Liam Morrison 
Organisation University of Edinburgh
Department The Roslin Institute
Country United Kingdom 
Sector Academic/University 
PI Contribution Analysis is single cattle B cell antibody sequences
Collaborator Contribution Single cattle B cell antibody sequences
Impact None as yet
Start Year 2019
 
Description Dr Lyndsay Fry 
Organisation Washington State University
Department Washington State University Spokane
Country United States 
Sector Academic/University 
PI Contribution Single B cell sorting and antibody sequencing, developing a new methods for Theileria sp.
Collaborator Contribution Providing funding and the samples from immunised animals. We have also engineered an antibody to aid in the sorting of cattle B cells
Impact None as yet
Start Year 2019
 
Description Dr Michael Skinner, Imperial College London 
Organisation Imperial College London
Department Department of Primary Care and Public Health
Country United Kingdom 
Sector Academic/University 
PI Contribution We aim to compare the host transcriptional response of primary chicken B cells infected with different strains of IBDV of varying virulence. We have the specialist reagents and expertise in house that are required to culture primary chicken B cells, and we have access to different strains of IBDV.
Collaborator Contribution Dr Michael Skinner and his team at Imperial College London have the expertise and facilities to generate and analyse RNA-Seq data in order to determine host transcriptional responses. This is required in order to complete the aim of the project.
Impact Further funding: PhD studentship (£102,800) - Dr Andrew Broadbent at The Pirbright Institute and Dr Michael Skinner at Imperial College London are joint supervisors for a PhD student working on this project. Further Funding: Houghton Trust (£8,000)- Dr Andrew Broadbent was awarded a Houghton Trust Small Research Grant to contribute to this project. Further funding: NC3Rs (Grant Ref: NC/R001138/1)
Start Year 2014
 
Description Dr Rachel Edgar, Imperial College London- defining the circadian clock in chicken cells and how this impacts upon viral replication 
Organisation Imperial College London
Country United Kingdom 
Sector Academic/University 
PI Contribution we will supply sequences of chicken clock genes and chicken cells and challenge synchronised chicken cells with IBDV and perform in vivo experiments
Collaborator Contribution Dr Edagr's group will synchronise chicken cells and characterise the oscillatory expression of chicken clock genes.
Impact we have been successful in obtaining funding (Houghton Trust, £4,500)
Start Year 2019
 
Description Efficacy of a candidate universal influenza vaccine, S-FLU. 
Organisation University of Oxford
Department Radcliffe Department of Medicine
Country United Kingdom 
Sector Academic/University 
PI Contribution Tested the immunogenicity and efficacy of a candidate universal influenza vaccine, S-FLU, in pigs. For the first time we demonstrated that S-FLU when administered by aerosol can reduce viral load in nasal swabs and lung in pigs after influenza virus challenge. We have shown that the most efficient way to administer this vaccine is by aerosol.
Collaborator Contribution Professor Alain Townsend has developed the S-FLU vaccine.
Impact The most efficient way to induce immune response in the lung is after aerosol delivery of LAIV vaccines in pigs. The most efficient way to induce cross-protective immunity is by aerosol delivery of S-FLU to the lungs of pigs
Start Year 2014
 
Description Engineering mosquitoes for reduced vector competence for chikungunya virus 
Organisation University of Malaya
Department Department of Medical Microbiology
Country Malaysia 
Sector Academic/University 
PI Contribution Developed joint research programme, led successful application for funding, coordinate programme.
Collaborator Contribution Various specific strands of research, and group discussions about results, conclusions, research progress and future activities.
Impact reported under funding
Start Year 2016
 
Description Engineering mosquitoes for reduced vector competence for chikungunya virus 
Organisation University of Melbourne
Country Australia 
Sector Academic/University 
PI Contribution Developed joint research programme, led successful application for funding, coordinate programme.
Collaborator Contribution Various specific strands of research, and group discussions about results, conclusions, research progress and future activities.
Impact reported under funding
Start Year 2016
 
Description Engineering mosquitoes for reduced vector competence for chikungunya virus 
Organisation University of Tartu
Department Institute of Technology
Country Estonia 
Sector Academic/University 
PI Contribution Developed joint research programme, led successful application for funding, coordinate programme.
Collaborator Contribution Various specific strands of research, and group discussions about results, conclusions, research progress and future activities.
Impact reported under funding
Start Year 2016
 
Description Establishment of a novel poultry vaccine platform inducing rapid and strong immunity through targeted delivery of antigens to chicken immune cells 
Organisation MSD Animal Health
Country United Kingdom 
Sector Private 
PI Contribution The research focus is to develop next-generation of poultry vaccines that induce rapid and strong immune responses in chickens. The candidate vaccine constricts will be developed at the Pirbright Institute
Collaborator Contribution The collaborating partner "MSD Animal Health" will evaluate the potency and efficacy for registration and feasibility for commercial production.
Impact Based on the project preliminary data a new project was developed entitled Protecting poultry from avian influenza, Newcastle disease, infectious bronchitis, and Gumboro disease with a single dose of a multivalent vaccine". This project further strengthened the collaborative research work with MSD Animal Health.
Start Year 2021
 
Description Evaluation of COVID-19 vaccine candidate immunogenicity in pigs 
Organisation Imperial College London
Country United Kingdom 
Sector Academic/University 
PI Contribution Expertise in evaluating vaccine immunogenicity in pigs
Collaborator Contribution Provided COVID-19 vaccine candidates
Impact Joint publication on the evaluation of ChAdOx1 nCoV-19 (AZD1222) (https://doi.org/10.1038/s41541-020-00221-3). Data included in in the pre-clinical dossier submitted to regulators (e.g., European Medicines Agency) and supported the successful Marketing Authorisation application.
Start Year 2020
 
Description Evaluation of COVID-19 vaccine candidate immunogenicity in pigs 
Organisation University of Oxford
Country United Kingdom 
Sector Academic/University 
PI Contribution Expertise in evaluating vaccine immunogenicity in pigs
Collaborator Contribution Provided COVID-19 vaccine candidates
Impact Joint publication on the evaluation of ChAdOx1 nCoV-19 (AZD1222) (https://doi.org/10.1038/s41541-020-00221-3). Data included in in the pre-clinical dossier submitted to regulators (e.g., European Medicines Agency) and supported the successful Marketing Authorisation application.
Start Year 2020
 
Description Evaluation of COVID-19 vaccine candidate immunogenicity in pigs 
Organisation University of Oxford
Country United Kingdom 
Sector Academic/University 
PI Contribution Expertise of using the pig as a model to evaluate vaccine immunogenicity.
Collaborator Contribution Provision of COVID-19 vaccine candidates
Impact The immunogenicity data of ChAdOx1 nCoV-19 in pigs was included in the pre-clinical dossier submitted to regulators (e.g., European Medicines Agency) and supported the successful Marketing Authorisation application. The immunogenicity data of RBD-SpyVLP in pigs helped support secure funding to further develop with vaccine candidate and to enter clinical trials. Published papers to date: https://www.nature.com/articles/s41541-020-00221-3 and https://www.nature.com/articles/s41467-020-20654-7
Start Year 2019
 
Description Evaluation of bovine herpesvirus-4 as a vaccine vector for pigs 
Organisation University of Parma
Country Italy 
Sector Academic/University 
PI Contribution Expertise in porcine vaccinology/immunology
Collaborator Contribution Expertise in the generation of recombinant bovine herpesvirus-4 (BoHV-4) vectors expressing heterologous antigens
Impact We demonstrated that immunisation of pigs with recombinant BoHV-4 vectors expressing Nipah virus glycoproteins are highly immunogenic in pigs. Most promising and as hypothesised, were the potent CD4 and CD8 T cell responses which were an order of greater than those induced by other viral vaccine vectors. These data have led to the external funding of projects by two companies to evaluate BoHV-4 vectors in the context of other porcine diseases.
Start Year 2018
 
Description Evaluation of immune checkpoint inhibitors as molecular adjuvants for improved PRRSV vaccines 
Organisation Leidos
Country United States 
Sector Private 
PI Contribution Expertise in PRRSV immunology
Collaborator Contribution Patented immune checkpoint inhibitors
Impact No yet
Start Year 2019
 
Description Evaluation of mRNA vectored vaccines in pigs 
Organisation University of Pennsylvania
Country United States 
Sector Academic/University 
PI Contribution Expertise in porcine immunology/vaccinology
Collaborator Contribution Expertise in mRNA vaccines
Impact We evaluated in pigs the immunogenicity an mRNA vector encoding a Nipah virus glycoprotein. This vaccine formulation induced high virus neutralising antibody titres (comparable with protein subunit in adjuvant) and T cell responses (comparable with viral vectors). We also utilised mRNA immunised pigs to isolate Nipah virus neutralising monoclonal antibodies.
Start Year 2018
 
Description Evaluation of multivalent viral hemorrhagic fever vaccine candidates immunogenicity in pigs 
Organisation Imperial College London
Country United Kingdom 
Sector Academic/University 
PI Contribution Supported the pre-clinical development of multivalent viral hemorrhagic fever vaccine candidates by evaluating their immunogenicity in pigs
Collaborator Contribution Conceived and produced vaccine candidates
Impact Still ongoing.
Start Year 2021
 
Description Evaluation of solid dose vaccines for livestock 
Organisation Enesi Pharma
Country United Kingdom 
Sector Private 
PI Contribution Expertise in PRRSV and vaccine evaluation
Collaborator Contribution Expertise in vaccine formulation
Impact Multidisciplinary collaboration between formulation scientist and viral immunologists.
Start Year 2019
 
Description Exploiting mosquito immunity for generating refractory mosquitoes 
Organisation The Pirbright Institute
Country United Kingdom 
Sector Academic/University 
PI Contribution I brought my expertise in mosquito immunology and viral evasion of innate immune responses in vertebrates and invertebrates. I provided intellectual input into the project and provided training for one research assistant.
Collaborator Contribution The partner (Luke Alphey, The Pirbright Institute) brought their expertise in mosquito transgenesis and molecular approaches to developing refractory mosquitoes that cannot transmit viruses. The partner also provided intellectual input into the project.
Impact The following publications are associated with this collaboration: DOI: 10.3390/v13112116
Start Year 2020
 
Description Factor H and influenza virus 
Organisation Brunel University London
Department Brunel Arts
Country United Kingdom 
Sector Academic/University 
PI Contribution Our research team are hosting a PhD student from Brunel University to facilitate the investigation of any interaction between influenza virus and complement factor H. We are providing reagents, expertise in handling influenza virus and advice on the scientific hypothesis of the research programme.
Collaborator Contribution Brunel university initiated the project and provided reagents including antibodies and methods for purification human co-factor H protein. They also provide funding for this PhD studentship.
Impact No outcomes as yet
Start Year 2018
 
Description Finn Grey - CRISPR and ISG screens 
Organisation University of Edinburgh
Department The Roslin Institute
Country United Kingdom 
Sector Academic/University 
PI Contribution My research team is providing expertise in coronavirus biology and regulation of the host cell environment. Laboratory experiments are being performed by my team.
Collaborator Contribution Finn's team have provided reagents and are advising on experimental design and data analysis.
Impact Cas-9 expressing avian cells have been generated and validated. Optimisation of the CRISPR screen is underway.
Start Year 2021
 
Description Gene editing chickens with Roslin 
Organisation University of Edinburgh
Department Roslin Institute Transgenic Chicken Facility
Country United Kingdom 
Sector Academic/University 
PI Contribution We have provided infectious pathogen challenge and infectious pathogen sample processing facilities in this collaboration. We infected the transgenic chickens with avian influenza virus and processed the samples following challenge.
Collaborator Contribution Helen Sang and Mike McGrew at The Roslin Institute (University of Edinburgh) contributed by producing and providing transgenic chickens (Split GFP) for challenge. Expertise in genotype screening the transgenic birds was shared with us.
Impact We have now successfully received a BBSRC responsive mode grant to carry out more transgenic bird challenges with influenza viruses. BB/S007911/1 -Investigating the role of ANP32A in the replication of avian Influenza virus.
Start Year 2018
 
Description Generation of immortalised bovine antigen presenting cells 
Organisation University of Surrey
Country United Kingdom 
Sector Academic/University 
PI Contribution Using a lentivirus vector expressing proto-oncogenic genes, we generated MHC-defined immortal bovine antigen presenting cells that can be used in antigen presentation studies or virus-host interaction studies in the future.
Collaborator Contribution The University of Surrey provided several lenvirus vectors and preparation for immortalization studies.
Impact A publication in progress titled "Generation of immortal bovine antigen presenting cells" and the generation of a library of MHC-defined bovine cell lines.
Start Year 2017
 
Description Horizon Discovery Ltd Cambridge Research Park, United Kingdom 
Organisation Horizon Discovery Group plc
Country United Kingdom 
Sector Private 
PI Contribution Verify IFITM knock-out cell by qPCR and immuno-blotting. Verification of further knock-out cell lines by DNA sequencing, immuno-blotting and qPCR Cell infection with Avian Influenza Viruses Assess the viral titre of cells infected with Influenza Viruses in K/O and wildtype cells. Quantitative analysis of viral infection in IFITM K/O cells Demonstrate increased AIV viral titres at lab scale and estimation of what this may equate to with respect to vaccine dose yields. Analysis of IFITM K/O cell lines permissivity to a range of additional animal viruses (Relevant to the LVIF). Milestone 7: Demonstrate permissivity increased and viral titres for a range of LVIF relevant viruses in the edited cell lines.
Collaborator Contribution Develop a CRISPR/cas9 gene editing system to specifically target the chIFITM locus in avian cell culture
Impact Grant funding
Start Year 2017
 
Description ILRI Research Collaboration (Kenya, March 2017) 
Organisation International Livestock Research Institute (ILRI)
Country Kenya 
Sector Charity/Non Profit 
PI Contribution Exchange of ideas and samples for indigenous breeds of livestock
Collaborator Contribution Contributed samples for analysis.
Impact Analysis of indigenous breed genomic sequences for Immune function genes.
Start Year 2017
 
Description Identification of conserved B-cell epitopes of highly pathogenic coronaviruses for broadspectrum immunotherapy and vaccine design. 
Organisation Imperial College London
Country United Kingdom 
Sector Academic/University 
PI Contribution We have performed experiments to generate preliminary data for a collaborative grant application, contributed to the writing of the grant proposal and responded to reviewers comments.
Collaborator Contribution My collaborators have performed experiments to generate preliminary data for a collaborative grant application, contributed to the writing of the grant proposal and responded to reviewers comments.
Impact Submission of proposal entitled "Identification of conserved B-cell epitopes of highly pathogenic coronaviruses for broadspectrum immunotherapy and vaccine design" to the "One Health Approaches to Accelerate Vaccine Development" call that forms part of the UK government's commitment to Official Development Assistance (ODA) in October 2017.
Start Year 2017
 
Description Identification of conserved B-cell epitopes of highly pathogenic coronaviruses for broadspectrum immunotherapy and vaccine design. 
Organisation University of Kent
Country United Kingdom 
Sector Academic/University 
PI Contribution We have performed experiments to generate preliminary data for a collaborative grant application, contributed to the writing of the grant proposal and responded to reviewers comments.
Collaborator Contribution My collaborators have performed experiments to generate preliminary data for a collaborative grant application, contributed to the writing of the grant proposal and responded to reviewers comments.
Impact Submission of proposal entitled "Identification of conserved B-cell epitopes of highly pathogenic coronaviruses for broadspectrum immunotherapy and vaccine design" to the "One Health Approaches to Accelerate Vaccine Development" call that forms part of the UK government's commitment to Official Development Assistance (ODA) in October 2017.
Start Year 2017
 
Description Identification of human orthologues of bovine Workshop Cluster 1 genes 
Organisation University of Oxford
Department Nuffield Department of Medicine
Country United Kingdom 
Sector Academic/University 
PI Contribution The Workshop Cluster 1 (WC1) gene family encodes a group of proteins thought to be involved in immune recognition. These genes are exclusively expressed on T cells expressing the gamma and delta T cell receptor and are well characterized in the bovine system. We have began investigating the presence and expression of WC1 on human cells using co-immunoprecipitation and mass spectrometry analysis of putatitve human WC1 genes.
Collaborator Contribution The Univ. of Oxford has developed a pipeline to identify protein fragments using mass spectrometry and bioinformatics from putative proteins from which little or no information is currently known. This pipeline has been applied to identify putative human WC1 proteins obtained from co-immunoprecipitation/mass spectrometry studies.
Impact The identification of one human WC1 gene/protein. A publication in progress titled "Identification of the human orthologue of a Workshop Cluster 1 gene expressed on gammadelta T cells"
Start Year 2017
 
Description Identification of sows with antibodies broadly neutralising PRRSV 
Organisation University of Lleida
Country Spain 
Sector Academic/University 
PI Contribution Expertise in PRRSV immunology
Collaborator Contribution Access to large numbers of sows routinely vaccinated and exposed to PRRSV
Impact Not yet
Start Year 2019
 
Description Imperial 
Organisation Imperial College London
Country United Kingdom 
Sector Academic/University 
PI Contribution Joint supervision of PhD student
Collaborator Contribution Joint supervision of PhD student
Impact One successful completed PhD student. Papers published or in preparation. Another PhD student in progress.
Start Year 2010
 
Description Influenza and co-factor H protein 
Organisation Leibniz Association
Department Leibniz Institute for Natural Product Research and Infection Biology
Country Germany 
Sector Academic/University 
PI Contribution We are screening influenza virus interaction with co-factor H proteins to determine the interaction interface.
Collaborator Contribution Christine Skerka has provided fragment factor H peptides to facilitate our mapping studies of the interaction surface with influenza viruses.
Impact No outcomes as yet
Start Year 2019
 
Description Investigating antigenic determinants inducing stronger and broader cross-protective immunity among H5 avian influenza viruses 
Organisation Royal Veterinary College (RVC)
Department Veterinary Basic Sciences
Country United Kingdom 
Sector Academic/University 
PI Contribution The overarching aims of the proposed research are to improve controls against H5 subtype of avian influenza viruses infecting poultry by investigating underlying mechanisms that define how vaccines renders effectiveness and develop novel approaches enhancing the effectiveness of H5 avian influenza vaccines. We recently generated a panel of monoclonal antibodies (mAbs) that recognise a variety of different clades of H5 viruses. Propagation of AIV in eggs or cultured cells together with mAbs drives virus to escape from mAbs neutralisation activity. These classical virus neutralization assays (VN) followed by HA gene sequence analysis allowed us to defined the role of amino acid substitutions that are associated with evasion of antibody neutralization through emergence of antigenic variants and failure of vaccine efficacy.
Collaborator Contribution provided intellectual input for achieving prescribed objectives of this work.
Impact Talk entitled "Molecular determinants for antigenicity and vaccine efficacy of avian influenza viruses" was presented at Newton Agham Researcher Links Workshop " Novel Vaccines and Diagnostic Technologies against Emerging and re-emerging Veterinary Pathogens" at Rizal Park Hotel, Manila, Philippines, 4-7 February 2019.
Start Year 2019
 
Description Investigating antigenic determinants inducing stronger and broader cross-protective immunity among H5 avian influenza viruses. 
Organisation Royal Veterinary College (RVC)
Country United Kingdom 
Sector Academic/University 
PI Contribution We developed research programme to characterise diversity of antigenic epitopes induce protective immunity in chickens against H5 subtypes of avian influenza viruses.
Collaborator Contribution Collaborating partner Dr Nicola Lewis at Royal Veterinary College provide advise to on cartography programme to visualize the antigenic similarity or difference among different strains of influenza viruses.
Impact The work under this collaborative determined the antigenic differences among different clades of H5 avian influenza viruses circulating in birds. This project is multi-disciplinary. our laboratory mainly work on virology and immunology part of the project and we seek help on computer modeling part from the collaborating partner Dr Nicola Lewis.
Start Year 2019
 
Description Investigating the role of the SH protein of RSV in the duration of immunity 
Organisation Imperial College London
Department Department of Medicine
Country United Kingdom 
Sector Academic/University 
PI Contribution We provided initial data supporting the hypothesis that the small hydrophobic protein of bovine respiratory syncytial virus regulates the host's immune response by modulating the transcription factors NF-kB and STAT1 in bovine cells
Collaborator Contribution Based on our results, Imperial has began investigating the role of the small hydrophobic protein of human respiratory syncytial virus on human cells.
Impact Publication in progress titled "Regulation of the B cell activating factor BAFF by the small hydrophobic protein of respiratory syncytial virus".
Start Year 2017
 
Description Invitation to participate in the Vaccine Innovation Forum 2017 at Shanghai 26-27 April 2017 
Organisation Chinese Academy of Agricultural Sciences
Department Shanghai Veterinary Research Institute
Country China 
Sector Public 
PI Contribution Prof. Nair was invited to participate in the Vaccine Innovation Forum 2017 at Shanghai 26-27 April 2017 to present a talk on 'Novel Vaccine Development for Avian Virus Diseases'. He also participated in the round table discussions on the challenges and opportunities in the vaccines and vaccination sector
Collaborator Contribution Vaccine Innovation Forum will help in expanding the future collaborations and activities of the UK-China Centre of Excellence for Research on Avian Diseases (http://www.uk-china-cerad.org/).
Impact organised joint meeting in China and the UK to discuss on challenges and opportunities
Start Year 2017
 
Description JN - Preparation of capped mesoporpus silica nanoparticles 
Organisation Polytechnic University of Valencia
Country Spain 
Sector Academic/University 
PI Contribution We were involved with testing the nanoparticles once they had been made. We also contributed purified antibody and shipped it to our collaborators for the generation of one type of nanoparticle, which we subsequently tested. We were involved with the intellectual design of experiments
Collaborator Contribution Our partners generated the nanoparticles and shipped them to us for testing. Our partners were also involved with the intellectual design of experiments.
Impact Experimental work performed to investigate the use of mesoporous silica nanoparticles to detect FMDV proteases in clinical samples
Start Year 2016
 
Description JS - Production of monoclonal antibodies that recognise FMDV VP4 from mouse spleens 
Organisation University of Edinburgh
Department The Roslin Institute
Country United Kingdom 
Sector Academic/University 
PI Contribution VLPs displaying the N-terminal 15 amino acids of FMDV were designed and produced at The Pirbright Institute and then mice were immunised with them. The spleens of the immunised mice were harvested and splenocytes stored. The response to the VP4 sequence was checked by ELISA and the mouse with the best response was selected to send the splenocytes for monoclonal production.
Collaborator Contribution The collaborators took the splenocytes obtained from the mouse experiments and carried out the fusions. They then screened the supernatants for positive wells that detected the VP4 sequence.
Impact Positive wells have been identified indicating some monoclonals that are specific for VP4 have been generated. These will be further screened for ability to recognise virus and to neutralise infection.
Start Year 2017
 
Description Janet Daly & Stephen Dunham- PI3K pathway and influenza 
Organisation University of Nottingham
Department School of Veterinary Medicine and Science Nottingham
Country United Kingdom 
Sector Academic/University 
PI Contribution Preliminary data was produced by Pirbright using reverse genetics of avian influenza viruses to secure a 50:50 funded PhD studentship that will commence in October 2020 entitled "Understanding the importance of the PI3K pathway in modulating influenza virus replication in chickens and ducks".
Collaborator Contribution Preliminary data was produced by University of Nottingham using PI3K p85 subunit pulldowns to secure a 50:50 funded PhD studentship that will commence in October 2020 entitled "Understanding the importance of the PI3K pathway in modulating influenza virus replication in chickens and ducks".
Impact Funding for a PhD studentship entitled "Understanding the importance of the PI3K pathway in modulating influenza virus replication in chickens and ducks".
Start Year 2019
 
Description Leeds FBS 
Organisation University of Leeds
Department Leeds Institute of Molecular Medicine
Country United Kingdom 
Sector Academic/University 
PI Contribution Research
Collaborator Contribution Research and student supervision
Impact Research
Start Year 2009
 
Description Linking sex determination and dosage compensation in a mosquito Anopheles gambiae 
Organisation University of Zurich
Department Institute of Molecular Life Sciences
Country Switzerland 
Sector Academic/University 
PI Contribution Developed a new research program.
Collaborator Contribution Provided intellectual input, contributed to drafting a joined grant proposal.
Impact No output yet.
Start Year 2019
 
Description Maria Harkiolaki - soft xray tomography 
Organisation Diamond Light Source
Country United Kingdom 
Sector Private 
PI Contribution The project aims to characterise coronavirus replication organelles in whole infected cells. My team provides significant experience in coronavirus replication organelles. The project is funded by a joint studentship and the student is, predominantly based in my laboratory.
Collaborator Contribution Maria's team provide extensive expertise in soft Xray tomography and are advising on experimental design and dat aanalysis.
Impact Preliminary experiments have been performed to visualise replication organelle membranes. Optimisation of experimental set up is underway.
Start Year 2020
 
Description Martin Ludlow - University of Veterinary Medicine, Hanover. Morbillivirus collaboration. 
Organisation University of Veterinary Medicine Hanover
Country Germany 
Sector Academic/University 
PI Contribution Provision of technical information and advice about the host-range of morbilliviruses.
Collaborator Contribution Provision of reagents to support our research project, e.g. plasmid clones of viral attachment proteins and receptors.
Impact No outcomes as yet, only preliminary data.
Start Year 2018
 
Description Martin Turner - The role of cellular RNA binding proteins in Coronavirus replication 
Organisation Babraham Institute
Country United Kingdom 
Sector Academic/University 
PI Contribution My lab has begun to establish the use of a CRISPR library targeting cellular RNA binding proteins. This includes selecting the most appropriate cells, virus and conditions for the screen and the readout method and this is based on our expertise and experience in coronavirus biology. All experimental work for the collaboration has been performed in my laboratory.
Collaborator Contribution Martin Turner has provided a CRISPR knockout library targeting human and murine RNA binding proteins. He has provided advice and protocols to allow us to establish the screen using human or murine coronaviruses and will advise on analysis of data following the screen.
Impact Experiments are still in initial set up stages and no results have been generated to date.
Start Year 2020
 
Description Miguel Ángel Muñoz Alía - Mayo clinic, USA. Measles population diversity. 
Organisation Mayo Clinic
Country United States 
Sector Charity/Non Profit 
PI Contribution Technical support and sharing of research data on the host-range of morbilliviruses.
Collaborator Contribution Provision of plasmid clones representing the broad genetic diversity of measles attachment and fusion proteins. Technical support.
Impact No outcomes as yet.
Start Year 2018
 
Description Miles Carroll PHE collaboration on pseudotyping 
Organisation Public Health England
Country United Kingdom 
Sector Public 
PI Contribution Provision of reagents to perform pseudotyping of Ebola G protein Hosting of visiting students to attempt sarbecovirus pseudotyping
Collaborator Contribution Discussion of preliminary data Provision of sarbecovirus constructs
Impact None to date, publications in preparation.
Start Year 2019
 
Description Mosquito Interest Group 
Organisation Imperial College London
Country United Kingdom 
Sector Academic/University 
PI Contribution Organising and attending meetings of students, RAs, postdocs and PIs on mosquito biology and related techniques.
Collaborator Contribution Organising and attending meetings of students, RAs, postdocs and PIs on mosquito biology and related techniques.
Impact Exchange of practical techniques, for instance in tracking oviposition of individual mosquitoes. Brainstorming for projects and papers, resulting in a GCRF VBD network proposal (which passed the first review but was not funded in the second) and an informal collaboration (which was intended for submission to UK-Thai call in 2017 but Thai partner was not eligible). Exchanges of colony strains of insects planned this month.
Start Year 2016
 
Description Mucosally Associated Invariant T cells (MAIT) in pig influenza model 
Organisation University of Oxford
Country United Kingdom 
Sector Academic/University 
PI Contribution Identified MAIT cells in pig Influenza model. Provide samples from infected and immunised protected animals
Collaborator Contribution Provide expertise in characterising MAIT: development of tetramer; Zell scanner technology allowing high resolution studies of cell phenotype and functions
Impact Joint PhD studentship with Paul Klenerman, University of Oxford
Start Year 2016
 
Description Next-generation PRRSV vaccines 
Organisation Eco Animal Health Ltd
Country United Kingdom 
Sector Private 
PI Contribution Know how in terms of genetically manipulating PRRSV and evaluating PRRSV vaccines.
Collaborator Contribution Financial contribution and know how in terms of developing a commercial vaccine.
Impact Too early.
Start Year 2020
 
Description Next-generation PRRSV vaccines 
Organisation Huvepharma
Country Bulgaria 
Sector Private 
PI Contribution Expertise in PRRSV virology and immunology
Collaborator Contribution Funding
Impact Too early, project ongoing.
Start Year 2019
 
Description Nicolas Locker - Investigation of translation regulation by infectious bronchitis virus 
Organisation University of Surrey
Country United Kingdom 
Sector Academic/University 
PI Contribution My team provide experience in molecular virology and cellular interactions of coronaviruses including previous experience of accessory proteins regulating cellular translation. One PhD student has successfully completed his studies working jointly between our labs and a second is due to begin in October 2022.
Collaborator Contribution Dr Locker's team provide expertise in viral regulation of cellular translation and stress granule signalling. Dr Locker is also providing protocols, advice and training for techniques including ribopuromycylation and ribosome profiling.
Impact PhD student funding (studentship Oct 2017- Sept 2021) - Control of host cell translation by infectious bronchitis virus PhD student funding (studentship Oct 2022- Mar 2026) - Coronavirus regulation of cellular antiviral responses and cross species transmission
Start Year 2016
 
Description Outreach Network for Gene Drive Research 
Organisation Outreach Network for Gene Drive Research
Sector Charity/Non Profit 
PI Contribution Input to policy discussions
Collaborator Contribution Network is engaged in briefing national and international entities, e.g. EU/EC, European Parliament, IUCN, Convention on Biological Diversity, on issues related to gene drive. Outreach also relates to general public (directly and via media) as well as policymakers, regulators and practitioners. The Pirbright Institute is now a member of the Network
Impact Briefing and media comment regarding gene drives and gene drive research to multiple agencies e.g. EU/EC, European Parliament, IUCN, Convention on Biological Diversity, on issues related to gene drive.
Start Year 2019
 
Description Oxford - Strubi 
Organisation University of Oxford
Country United Kingdom 
Sector Academic/University 
PI Contribution Collaboration on virus structural biology and joint PhD studentship
Collaborator Contribution Collaboration on virus structural biology and joint PhD studentship
Impact Collaboration on virus structural biology has produced a number of novel virus structures and new understanding of virus entry and packaging
Start Year 2008
 
Description Oxford University Collaborations 
Organisation University of Oxford
Department Big Data Institute
Country United Kingdom 
Sector Academic/University 
PI Contribution Scientific discussion, collaboration and manuscript preparation
Collaborator Contribution Scientific discussion, collaboration and manuscript preparation
Impact Manuscript in preparation
Start Year 2017
 
Description Parham Lab at Stanford 
Organisation Stanford University
Department School of Medicine
Country United States 
Sector Academic/University 
PI Contribution Our group designed the experiments and generated a majority of the data. We also led the analysis of the data and writing of the manuscripts
Collaborator Contribution The Parham lab is the leading group worldwide in the evolutionary study of NK cell receptors and MHC class I. This group generated part of the data and helped in the analysis, interpretation and manuscript preparation
Impact Definition of the Cattle Killer Cell Igndash like Receptor Gene Family: Comparison with Aurochs and Human Counterparts Nicholas D. Sanderson, Paul J. Norman, Lisbeth A. Guethlein, Shirley Ellis, Christina Williams, Matthew Breen, Steven D. E. Park, David A. Magee, Farbod Babrzadeh, Andrew Warry, Mick Watson, Daniel G. Bradley, David E. MacHugh, Peter Parham, and John A. Hammond. Journal of Immunology, Dec 15, 2014; 193 (12) This collaboration is not multi-didciplinary
Start Year 2009
 
Description Partnering with National Taiwan University 
Organisation National Taiwan University
Country Taiwan, Province of China 
Sector Academic/University 
PI Contribution We have prepared and submitted an application for a BBSRC-Taiwan Partnering Award to study "Assembly of Recombinant Infectious Bronchitis Virus and Determination of Antigenic Sites that Confer Hemagglutination Activity".
Collaborator Contribution The partners conceived the project and got in contact about submitting an application for funding together.
Impact An application has been made for a BBSRC-Taiwan Partnering Award in November 2018.
Start Year 2018
 
Description Paul Digard & Reidun Twarock - Packaging signals 
Organisation University of Edinburgh
Department The Roslin Institute
Country United Kingdom 
Sector Academic/University 
PI Contribution Supply of reverse genetic avian influenza viruses for the successful award of a PhD studentship based at The Roslin Institute entitled "Identification of interaction sites between the genomic segments of influenza virus as putative novel anti-viral targets".
Collaborator Contribution University of York is providing mathematical modelling algorithms of viral packaging. Paul Digard- Roslin Institute is providing influenza virology expertise and hosting the student.
Impact Studentship commenced in October 2019
Start Year 2019
 
Description Paul Digard & Reidun Twarock - Packaging signals 
Organisation University of York
Country United Kingdom 
Sector Academic/University 
PI Contribution Supply of reverse genetic avian influenza viruses for the successful award of a PhD studentship based at The Roslin Institute entitled "Identification of interaction sites between the genomic segments of influenza virus as putative novel anti-viral targets".
Collaborator Contribution University of York is providing mathematical modelling algorithms of viral packaging. Paul Digard- Roslin Institute is providing influenza virology expertise and hosting the student.
Impact Studentship commenced in October 2019
Start Year 2019
 
Description Paul Digard - The Roslin Insititute 
Organisation University of Edinburgh
Department The Roslin Institute
Country United Kingdom 
Sector Academic/University 
PI Contribution We generated constructs that express PB1-F2 from a variety of avian influenza strains in eukaryotic expression plasmids. We generated Chimera PB1-F2 expression plasmids where the C-terminus of the PB1-F2 are switched between strains. Using these plasmids we have determined the localization and antagonist activity toward the IFN beta signaling pathway and NF-kb signaling pathway in human and chicken cells. We have therefore provided reagents and intellectual input to the collaboration.
Collaborator Contribution Our collaborators have used these plasmids in there well defined stability assays to determine sequence specific effects on this characteristic. Intellectual input and expertise in a particular assay were made by these collaborators.
Impact We have successfully published some of this work; "The cellular localization of avian influenza virus PB1-F2 protein alters the magnitude of IFN2 promoter and NF?B-dependent promoter antagonism in chicken cells." doi: 10.1099/jgv.0.001220. We have an ongoing joint PhD studentship in this area. This collaboration remains current and active.
Start Year 2017
 
Description Paul Verkade - Electron tomography and CLEM 
Organisation University of Bristol
Department Avon Longitudinal Study of Parents and Children (ALSPAC)
Country United Kingdom 
Sector Academic/University 
PI Contribution Nicole Doyle has performed electron tomography to analyse the three dimensional structure of membrane rearrangements induced by the expression of IBV non-structural proteins 4 or 3 and 4 in the absence of other viral components. Sample set up was performed at Pirbright and analysis was subsequently performed at University of Bristol followed by additional analysis at Pirbright.
Collaborator Contribution Paul has assisted with electron tomography data generation providing access to electron microscopes in Bristol as well as training for Nicole Doyle in performing experiments and analysing and modelling data. He is also advising Nicole while establishing correlative light electron microscopy at Pirbright.
Impact doi: 10.3390/v10090477
Start Year 2018
 
Description Pirbright-Diamond collaboration 
Organisation Diamond Light Source
Country United Kingdom 
Sector Private 
PI Contribution 50% of my time is now based at Diamond, I am considered in-house staff and can apply to use the instrumentation. I feed projects to eBIC for collaboration using advanced electron microscopy, centered around cell biology of host-pathogen interactions using advanced electron microscopy, plus potential use of Xray microscopy (on application).
Collaborator Contribution Instrument time on cryo-FIB-SEM and screening TEM (two days per month). Diamond have loaned us a plunge freezing device.
Impact No outputs yet
Start Year 2019
 
Description Professor Benedikt Kaufer 
Organisation Free University of Berlin
Country Germany 
Sector Academic/University 
PI Contribution Providing knowledge and methodologies.
Collaborator Contribution Providing reagents and methodologies to perform experiments.
Impact Two publications from this collaboration: Gurung A, Kamble N, Kaufer BB, Pathan A, Shahriar Behboudi. Association of Marek's Disease induced immunosuppression with activation of a novel regulatory T cells in chickens, PLoS Pathogens, 2017, 13 (12), e1006745. Boodhoo N, Kamble N, Kaufer BB, Shahriar Behboudi. Replication of Marek's disease virus is dependent on de novo synthesis of fatty acid and Prostaglandin E2. J Virol. 2019 Apr 10
Start Year 2019
 
Description Professor Helen Sang,The Roslin Institute - GFP1-10 expressing transgenic chickens 
Organisation University of Edinburgh
Department The Roslin Institute
Country United Kingdom 
Sector Academic/University 
PI Contribution We have developed IBDV tagged to GFP11
Collaborator Contribution Helen Sang's group at the Roslin Institute have developed transcgenic chicken lines that constitutively express GFP1-10 in every cell.
Impact we have been successful in obtaining funding (Grant Ref: BB/S014594/1)
Start Year 2019
 
Description Professor Jim Kaufman 
Organisation University of Edinburgh
Country United Kingdom 
Sector Academic/University 
PI Contribution Provided information on CD4 T cell epitopes derived from Marek's disease virus
Collaborator Contribution Provided knowledge and information on assessing peptide binding to chicken MHC molecules
Impact Generation of data leading to understanding correlate of protection against Marek's disease virus.
Start Year 2019
 
Description Professor Shayan Sharif, University of Guelph, Canada 
Organisation University of Guelph
Department Department of Pathobiology
Country Canada 
Sector Academic/University 
PI Contribution Collaboration between my team and Professor Sharif lead to new finding in Avian immunology, specifically in understanding the effects of nutrients on antigen presenting cells and T cells of chicken. We took the lead in studying the effects of some nutrients (Vitamin D) on the function of chicken T cells, performed most of the experiments in our laboratory. Meanwhile, we participated in studying the effects of Vitamin D on antigen presenting cells of chicken by performing some key experiments because we had access to some specific reagents (monoclonal antibodies) recognizing molecules expressed on chicken antigen presenting cells.
Collaborator Contribution Professor Sharif team took the lead in studying the effects of Vitamin D on antigen presenting cells of chicken, and contributed to experimental plan for studying the effects on chicken T cells.
Impact Two publications in 2015 and 2016: 1. Nitish Boodhoo, Shayan Sharif, Shahriar Behboudi. 1a,25(OH)2 Vitamin D3 Modulates Avian T Lymphocyte Functions without Inducing CTL Unresponsiveness. PLoS One. 2016 Feb 24;11(2):e0150134 2. Bahram Shojadoost, Shahriar Behboudi, Villanueva AI, Jennifer Brisbin, Ali Ashkar, Shayan Sharif. Vitamin D3 modulates the function of chicken macrophages. Res. Vet. Sci. 2015 June, 100: 45-51
Start Year 2019
 
Description Protein Production UK (Formerly the Oxford Protein Production Facility) 
Organisation Rosalind Franklin Institute
Country United Kingdom 
Sector Charity/Non Profit 
PI Contribution We are providing the sequences of antibodies from various species to add into the vectors at Oxford to produce species specific antibodies. We are also providing and devloping assays to measure recombinant antibody confirmation and binding.
Collaborator Contribution PPUK is providing access to all their facilities as well as expertise. They are also providing their vector backbone as the template for antibody production.
Impact None as yet
Start Year 2018
 
Description Pseudoparticles of influenza for interaction studies with co-factor H 
Organisation University of Kent
Department Medway School of Pharmacy
Country United Kingdom 
Sector Hospitals 
PI Contribution We have screened the interaction between influenza pseudotype particles that only contain the surface HA protein from influenza viruses for interaction with human co-factor H protein. We have screen pseudotype particles of multiple influenza subtypes.
Collaborator Contribution Nigel Temperton of the viral Pseudotype Unit at the School of Pharmacy provided influenza virus pseudotype particles of multiple subtypes to allow us to screen for interaction with co-factor H protein.
Impact No outcomes yet
Start Year 2018
 
Description Pseudotyping virus collaboration with Dr. Tess Lambe, University of Oxford 
Organisation University of Oxford
Country United Kingdom 
Sector Academic/University 
PI Contribution Development of assays and reagents to support successful pseudotyping of various viruses for quantitative entry assays
Collaborator Contribution Provision of reagents and plasmid clones
Impact No outputs yet,
Start Year 2019
 
Description Rapid acquisition of mammalian characteristics by avian influenza virus in single host infections. 
Organisation Imperial College London
Country United Kingdom 
Sector Academic/University 
PI Contribution Provided reagents and day to day technical help for undertaking this project and supervising postdoctoral researcher working on this project. Reagents include influenza reverse genetic plasmids and H9N2 and H7N9-specific antibodies.
Collaborator Contribution this project will aim to understand AIV genetics that facilitate the rapid acquisition of mammalian adaptation characteristics in a single host infection. The partners with our collaborations determined adaptive genetic changes in the H9N2 avian influenza virus following infection in mice (mammalian hosts). Different strains of avian influenza Viruses generated by reverse gentic techniques were inoculated into mice and lungs sampled daily. RNA recovered from lung homogenates were deep sequenced and mutations arising were characterised for adaptation. Relative viral fitness and the rapidity of the accumulation of mutations was measured and compared amongst the viral strains.
Impact The results of the our experimental studies showed the avian-origin viruses rapidly acquired mutations that increase virus fitness in mammalian species. Therefore, these viruses pose zoonotic and pandemic threat to public health.
Start Year 2018
 
Description Rapid acquisition of mammalian characteristics by avian influenza virus in single host infections. 
Organisation The Pirbright Institute
Country United Kingdom 
Sector Academic/University 
PI Contribution Provided reagents and day to day technical help for undertaking this project and supervising postdoctoral researcher working on this project. Reagents include influenza reverse genetic plasmids and H9N2 and H7N9-specific antibodies.
Collaborator Contribution this project will aim to understand AIV genetics that facilitate the rapid acquisition of mammalian adaptation characteristics in a single host infection. The partners with our collaborations determined adaptive genetic changes in the H9N2 avian influenza virus following infection in mice (mammalian hosts). Different strains of avian influenza Viruses generated by reverse gentic techniques were inoculated into mice and lungs sampled daily. RNA recovered from lung homogenates were deep sequenced and mutations arising were characterised for adaptation. Relative viral fitness and the rapidity of the accumulation of mutations was measured and compared amongst the viral strains.
Impact The results of the our experimental studies showed the avian-origin viruses rapidly acquired mutations that increase virus fitness in mammalian species. Therefore, these viruses pose zoonotic and pandemic threat to public health.
Start Year 2018
 
Description Reannotation of the Anopheles gambiae genome 
Organisation Biomathematics and Statistics Scotland (BioSS)
Country United Kingdom 
Sector Public 
PI Contribution Generated and provided RNA-seq data from the developmental transcriptome of the mosquito Anopheles gambiae Manually browsed the transcriptomic data in the genome context and provided coordinates for genome regions with not annotated genes or genes requiring annotation corrections. Generated and provided FAIRE-seq data from embryo samples of Anopheles gambiae to identify regulatory elements controlling early embryo development.
Collaborator Contribution Conducting reannotation of the Anopheles gambiae genome based on RNA-seq data from the developmental transcriptome. Mapping the FAIRE-seq data to the genome and linking the mapped data the RNA-seq data.
Impact No outputs yet
Start Year 2017
 
Description Reannotation of the Anopheles gambiae genome 
Organisation Centre for Genomic Regulation (CRG)
Country Spain 
Sector Academic/University 
PI Contribution Generated and provided RNA-seq data from the developmental transcriptome of the mosquito Anopheles gambiae Manually browsed the transcriptomic data in the genome context and provided coordinates for genome regions with not annotated genes or genes requiring annotation corrections. Generated and provided FAIRE-seq data from embryo samples of Anopheles gambiae to identify regulatory elements controlling early embryo development.
Collaborator Contribution Conducting reannotation of the Anopheles gambiae genome based on RNA-seq data from the developmental transcriptome. Mapping the FAIRE-seq data to the genome and linking the mapped data the RNA-seq data.
Impact No outputs yet
Start Year 2017
 
Description Reannotation of the Anopheles gambiae genome 
Organisation Imperial College London
Country United Kingdom 
Sector Academic/University 
PI Contribution Generated and provided RNA-seq data from the developmental transcriptome of the mosquito Anopheles gambiae Manually browsed the transcriptomic data in the genome context and provided coordinates for genome regions with not annotated genes or genes requiring annotation corrections. Generated and provided FAIRE-seq data from embryo samples of Anopheles gambiae to identify regulatory elements controlling early embryo development.
Collaborator Contribution Conducting reannotation of the Anopheles gambiae genome based on RNA-seq data from the developmental transcriptome. Mapping the FAIRE-seq data to the genome and linking the mapped data the RNA-seq data.
Impact No outputs yet
Start Year 2017
 
Description Research Collaboration with Nigeria (23rd - 28th January 2017) 
Organisation University of Nigeria
Country Nigeria 
Sector Academic/University 
PI Contribution Analysis of rare breed indigenous chicken in Nigeria.
Collaborator Contribution Helped with sampling and background information of the breed.
Impact Analysis is ongoing.
Start Year 2017
 
Description Roslin - Grey 
Organisation University of Edinburgh
Department The Roslin Institute
Country United Kingdom 
Sector Academic/University 
PI Contribution Co-investigator in BBSRC project grant Collaboration in genetic screens to understand virus host interactions
Collaborator Contribution Co-investigator in BBSRC project grant Collaboration in genetic screens to understand virus host interactions
Impact Co-investigator in BBSRC project grant Collaboration in genetic screens to understand virus host interactions
Start Year 2019
 
Description Stathis Giotis - Bat immunity and differential gene expression 
Organisation University of Essex
Country United Kingdom 
Sector Academic/University 
PI Contribution The project aims to understand the role of viral regulation of innate immune signalling in coronavirus cross-species transmission, My research team provides expertise in coronavirus biology and host cell interaction, including reverse genetics. Laboratory experiments associated with this project are conducted by my team.
Collaborator Contribution Stathis has provided bat cells and bat interferon reporter plasmids, as well as advice and expertise, to perform experiments. He will also perform data analysis of planned experiments to interrogate differential gene expression of cells infected with different viruses.
Impact To early in the project to say.
Start Year 2020
 
Description Stephen Graham collaboration on structural biology with University of Cambridge 
Organisation University of Cambridge
Country United Kingdom 
Sector Academic/University 
PI Contribution Provision of data and sharing of experimental ideas regarding viral host range and techniques for simulating virus evolution
Collaborator Contribution Structural analysis of mutants to viral glycoproteins
Impact Journal of Virology paper on potential PPRV emergence in human populations (Abdullah et al, 2018) Application of error-prone PCR to functionally probe the morbillivirus Haemagglutinin protein. Gallo G, Conceicao C, Tsirigoti C, Willett B, Graham S and Bailey D. Journal of General Virology. J Gen Virol 2021 Apr;102(4). doi: 10.1099/jgv.0.001580. https://pubmed.ncbi.nlm.nih.gov/33739251/ The SARS-CoV-2 Spike protein has a broad tropism for mammalian ACE2 proteins. Conceicao C, Thakur N, Human S, Kelly JT, Logan L, Bialy D, Bhat S, Stevenson-Leggett P, Zagrajek AK, Hollinghurst P, Varga M, Tsirigoti C, Tully M, Chiu C, Moffat K, Silesian AP, Hammond JA, Maier HJ, Bickerton E, Shelton H, Dietrich I, Graham SC, Bailey D. PLoS Biology. 2020 Dec 21;18(12):e3001016. doi: 10.1371/journal.pbio.3001016. eCollection 2020 Dec. https://pubmed.ncbi.nlm.nih.gov/33347434/
Start Year 2018
 
Description Steve Goodbourn - St Georges - chicken innate molecule interactions. 
Organisation Ulster University
Department Biomedical Sciences Research Centre
Country United Kingdom 
Sector Academic/University 
PI Contribution We have collaborated by producing yeast- 2-hybrid plasmids containing the influenza PB1-F2 gene from various avian influenza strains. The PhD student on this studentship then visited Professor Goodbourn's laboratory to carry out the yeast-2-hybrid screen for interaction innate chicken molecules.
Collaborator Contribution Professor Goodbourn's laboratory provided yeast-2-hybrid plasmids containing various innate chicken molecules to be screened for interaction with avian influenza virus PB1-F2 protein. They also offered help and guidance carrying out the interaction technique to understand if PB1-F2 directly associated with chicken innate components. They also provided reporter plasmids and guidance on innate reporter assays in chicken cells.
Impact Successful publication of work. doi: 10.1099/jgv.0.001220. The cellular localization of avian influenza virus PB1-F2 protein alters the magnitude of IFN2 promoter and NF?B-dependent promoter antagonism in chicken cells.
Start Year 2016
 
Description Supply of Arthropods to External Partners 
Organisation Animal and Plant Health Agency
Country United Kingdom 
Sector Public 
PI Contribution Culicoides nubeculosus, C. sonorensis (biting midge) and Culex pipiens (mosquito) provided as pupae for insecticide, repellent and vector competence trials as a resource. UK supplies conducted under National Capability Grant. Approximately 30 000 insects shipped during reporting period for academic users. All insect production took place at Pirbright and training in maintenance was provided to a worker from FLI (Germany).
Collaborator Contribution Daughter line of C. sonorensis was established at FLI (improving security around sustaining the lines). Training and advice on insect maintenance and handling was provided by Pirbright to LSHTM, Liverpool and Reading. Studies then carried out included investigation of bacteria in Culicoides (University of Liverpool), modeling of mosquito populations (Reading) and development of novel insecticides and repellent formulations (LSHTM). These specialist expertise were driven by the host organizations for the studies.
Impact Products are still in development.
Start Year 2017
 
Description Supply of Arthropods to External Partners 
Organisation Bern University of Applied Sciences
Country Switzerland 
Sector Academic/University 
PI Contribution Culicoides nubeculosus, C. sonorensis (biting midge) and Culex pipiens (mosquito) provided as pupae for insecticide, repellent and vector competence trials as a resource. UK supplies conducted under National Capability Grant. Approximately 30 000 insects shipped during reporting period for academic users. All insect production took place at Pirbright and training in maintenance was provided to a worker from FLI (Germany).
Collaborator Contribution Daughter line of C. sonorensis was established at FLI (improving security around sustaining the lines). Training and advice on insect maintenance and handling was provided by Pirbright to LSHTM, Liverpool and Reading. Studies then carried out included investigation of bacteria in Culicoides (University of Liverpool), modeling of mosquito populations (Reading) and development of novel insecticides and repellent formulations (LSHTM). These specialist expertise were driven by the host organizations for the studies.
Impact Products are still in development.
Start Year 2017
 
Description Supply of Arthropods to External Partners 
Organisation ETH Zurich
Country Switzerland 
Sector Academic/University 
PI Contribution Culicoides nubeculosus, C. sonorensis (biting midge) and Culex pipiens (mosquito) provided as pupae for insecticide, repellent and vector competence trials as a resource. UK supplies conducted under National Capability Grant. Approximately 30 000 insects shipped during reporting period for academic users. All insect production took place at Pirbright and training in maintenance was provided to a worker from FLI (Germany).
Collaborator Contribution Daughter line of C. sonorensis was established at FLI (improving security around sustaining the lines). Training and advice on insect maintenance and handling was provided by Pirbright to LSHTM, Liverpool and Reading. Studies then carried out included investigation of bacteria in Culicoides (University of Liverpool), modeling of mosquito populations (Reading) and development of novel insecticides and repellent formulations (LSHTM). These specialist expertise were driven by the host organizations for the studies.
Impact Products are still in development.
Start Year 2017
 
Description Supply of Arthropods to External Partners 
Organisation Friedrich Loeffler Institute
Country Germany 
Sector Academic/University 
PI Contribution Culicoides nubeculosus, C. sonorensis (biting midge) and Culex pipiens (mosquito) provided as pupae for insecticide, repellent and vector competence trials as a resource. UK supplies conducted under National Capability Grant. Approximately 30 000 insects shipped during reporting period for academic users. All insect production took place at Pirbright and training in maintenance was provided to a worker from FLI (Germany).
Collaborator Contribution Daughter line of C. sonorensis was established at FLI (improving security around sustaining the lines). Training and advice on insect maintenance and handling was provided by Pirbright to LSHTM, Liverpool and Reading. Studies then carried out included investigation of bacteria in Culicoides (University of Liverpool), modeling of mosquito populations (Reading) and development of novel insecticides and repellent formulations (LSHTM). These specialist expertise were driven by the host organizations for the studies.
Impact Products are still in development.
Start Year 2017
 
Description Supply of Arthropods to External Partners 
Organisation Lancaster University
Country United Kingdom 
Sector Academic/University 
PI Contribution Culicoides nubeculosus, C. sonorensis (biting midge) and Culex pipiens (mosquito) provided as pupae for insecticide, repellent and vector competence trials as a resource. UK supplies conducted under National Capability Grant. Approximately 30 000 insects shipped during reporting period for academic users. All insect production took place at Pirbright and training in maintenance was provided to a worker from FLI (Germany).
Collaborator Contribution Daughter line of C. sonorensis was established at FLI (improving security around sustaining the lines). Training and advice on insect maintenance and handling was provided by Pirbright to LSHTM, Liverpool and Reading. Studies then carried out included investigation of bacteria in Culicoides (University of Liverpool), modeling of mosquito populations (Reading) and development of novel insecticides and repellent formulations (LSHTM). These specialist expertise were driven by the host organizations for the studies.
Impact Products are still in development.
Start Year 2017
 
Description Supply of Arthropods to External Partners 
Organisation London School of Hygiene and Tropical Medicine (LSHTM)
Country United Kingdom 
Sector Academic/University 
PI Contribution Culicoides nubeculosus, C. sonorensis (biting midge) and Culex pipiens (mosquito) provided as pupae for insecticide, repellent and vector competence trials as a resource. UK supplies conducted under National Capability Grant. Approximately 30 000 insects shipped during reporting period for academic users. All insect production took place at Pirbright and training in maintenance was provided to a worker from FLI (Germany).
Collaborator Contribution Daughter line of C. sonorensis was established at FLI (improving security around sustaining the lines). Training and advice on insect maintenance and handling was provided by Pirbright to LSHTM, Liverpool and Reading. Studies then carried out included investigation of bacteria in Culicoides (University of Liverpool), modeling of mosquito populations (Reading) and development of novel insecticides and repellent formulations (LSHTM). These specialist expertise were driven by the host organizations for the studies.
Impact Products are still in development.
Start Year 2017
 
Description Supply of Arthropods to External Partners 
Organisation Queen's University Belfast
Country United Kingdom 
Sector Academic/University 
PI Contribution Culicoides nubeculosus, C. sonorensis (biting midge) and Culex pipiens (mosquito) provided as pupae for insecticide, repellent and vector competence trials as a resource. UK supplies conducted under National Capability Grant. Approximately 30 000 insects shipped during reporting period for academic users. All insect production took place at Pirbright and training in maintenance was provided to a worker from FLI (Germany).
Collaborator Contribution Daughter line of C. sonorensis was established at FLI (improving security around sustaining the lines). Training and advice on insect maintenance and handling was provided by Pirbright to LSHTM, Liverpool and Reading. Studies then carried out included investigation of bacteria in Culicoides (University of Liverpool), modeling of mosquito populations (Reading) and development of novel insecticides and repellent formulations (LSHTM). These specialist expertise were driven by the host organizations for the studies.
Impact Products are still in development.
Start Year 2017
 
Description Supply of Arthropods to External Partners 
Organisation Texas A&M University-Central Texas
Country United States 
Sector Academic/University 
PI Contribution Culicoides nubeculosus, C. sonorensis (biting midge) and Culex pipiens (mosquito) provided as pupae for insecticide, repellent and vector competence trials as a resource. UK supplies conducted under National Capability Grant. Approximately 30 000 insects shipped during reporting period for academic users. All insect production took place at Pirbright and training in maintenance was provided to a worker from FLI (Germany).
Collaborator Contribution Daughter line of C. sonorensis was established at FLI (improving security around sustaining the lines). Training and advice on insect maintenance and handling was provided by Pirbright to LSHTM, Liverpool and Reading. Studies then carried out included investigation of bacteria in Culicoides (University of Liverpool), modeling of mosquito populations (Reading) and development of novel insecticides and repellent formulations (LSHTM). These specialist expertise were driven by the host organizations for the studies.
Impact Products are still in development.
Start Year 2017
 
Description Supply of Arthropods to External Partners 
Organisation University of Bristol
Country United Kingdom 
Sector Academic/University 
PI Contribution Culicoides nubeculosus, C. sonorensis (biting midge) and Culex pipiens (mosquito) provided as pupae for insecticide, repellent and vector competence trials as a resource. UK supplies conducted under National Capability Grant. Approximately 30 000 insects shipped during reporting period for academic users. All insect production took place at Pirbright and training in maintenance was provided to a worker from FLI (Germany).
Collaborator Contribution Daughter line of C. sonorensis was established at FLI (improving security around sustaining the lines). Training and advice on insect maintenance and handling was provided by Pirbright to LSHTM, Liverpool and Reading. Studies then carried out included investigation of bacteria in Culicoides (University of Liverpool), modeling of mosquito populations (Reading) and development of novel insecticides and repellent formulations (LSHTM). These specialist expertise were driven by the host organizations for the studies.
Impact Products are still in development.
Start Year 2017
 
Description Supply of Arthropods to External Partners 
Organisation University of Greenwich
Department Natural Resources Institute Greenwich
Country United Kingdom 
Sector Academic/University 
PI Contribution Culicoides nubeculosus, C. sonorensis (biting midge) and Culex pipiens (mosquito) provided as pupae for insecticide, repellent and vector competence trials as a resource. UK supplies conducted under National Capability Grant. Approximately 30 000 insects shipped during reporting period for academic users. All insect production took place at Pirbright and training in maintenance was provided to a worker from FLI (Germany).
Collaborator Contribution Daughter line of C. sonorensis was established at FLI (improving security around sustaining the lines). Training and advice on insect maintenance and handling was provided by Pirbright to LSHTM, Liverpool and Reading. Studies then carried out included investigation of bacteria in Culicoides (University of Liverpool), modeling of mosquito populations (Reading) and development of novel insecticides and repellent formulations (LSHTM). These specialist expertise were driven by the host organizations for the studies.
Impact Products are still in development.
Start Year 2017
 
Description Supply of Arthropods to External Partners 
Organisation University of Liverpool
Department School of Veterinary Science Liverpool
Country United Kingdom 
Sector Academic/University 
PI Contribution Culicoides nubeculosus, C. sonorensis (biting midge) and Culex pipiens (mosquito) provided as pupae for insecticide, repellent and vector competence trials as a resource. UK supplies conducted under National Capability Grant. Approximately 30 000 insects shipped during reporting period for academic users. All insect production took place at Pirbright and training in maintenance was provided to a worker from FLI (Germany).
Collaborator Contribution Daughter line of C. sonorensis was established at FLI (improving security around sustaining the lines). Training and advice on insect maintenance and handling was provided by Pirbright to LSHTM, Liverpool and Reading. Studies then carried out included investigation of bacteria in Culicoides (University of Liverpool), modeling of mosquito populations (Reading) and development of novel insecticides and repellent formulations (LSHTM). These specialist expertise were driven by the host organizations for the studies.
Impact Products are still in development.
Start Year 2017
 
Description Supply of Arthropods to External Partners 
Organisation University of Reading
Department School of Biological Sciences Reading
Country United Kingdom 
Sector Academic/University 
PI Contribution Culicoides nubeculosus, C. sonorensis (biting midge) and Culex pipiens (mosquito) provided as pupae for insecticide, repellent and vector competence trials as a resource. UK supplies conducted under National Capability Grant. Approximately 30 000 insects shipped during reporting period for academic users. All insect production took place at Pirbright and training in maintenance was provided to a worker from FLI (Germany).
Collaborator Contribution Daughter line of C. sonorensis was established at FLI (improving security around sustaining the lines). Training and advice on insect maintenance and handling was provided by Pirbright to LSHTM, Liverpool and Reading. Studies then carried out included investigation of bacteria in Culicoides (University of Liverpool), modeling of mosquito populations (Reading) and development of novel insecticides and repellent formulations (LSHTM). These specialist expertise were driven by the host organizations for the studies.
Impact Products are still in development.
Start Year 2017
 
Description Supply of Arthropods to External Partners 
Organisation Wageningen University & Research
Country Netherlands 
Sector Academic/University 
PI Contribution Culicoides nubeculosus, C. sonorensis (biting midge) and Culex pipiens (mosquito) provided as pupae for insecticide, repellent and vector competence trials as a resource. UK supplies conducted under National Capability Grant. Approximately 30 000 insects shipped during reporting period for academic users. All insect production took place at Pirbright and training in maintenance was provided to a worker from FLI (Germany).
Collaborator Contribution Daughter line of C. sonorensis was established at FLI (improving security around sustaining the lines). Training and advice on insect maintenance and handling was provided by Pirbright to LSHTM, Liverpool and Reading. Studies then carried out included investigation of bacteria in Culicoides (University of Liverpool), modeling of mosquito populations (Reading) and development of novel insecticides and repellent formulations (LSHTM). These specialist expertise were driven by the host organizations for the studies.
Impact Products are still in development.
Start Year 2017
 
Description Supply of Arthropods to External Partners 
Organisation Zoological Society of London
Country United Kingdom 
Sector Charity/Non Profit 
PI Contribution Culicoides nubeculosus, C. sonorensis (biting midge) and Culex pipiens (mosquito) provided as pupae for insecticide, repellent and vector competence trials as a resource. UK supplies conducted under National Capability Grant. Approximately 30 000 insects shipped during reporting period for academic users. All insect production took place at Pirbright and training in maintenance was provided to a worker from FLI (Germany).
Collaborator Contribution Daughter line of C. sonorensis was established at FLI (improving security around sustaining the lines). Training and advice on insect maintenance and handling was provided by Pirbright to LSHTM, Liverpool and Reading. Studies then carried out included investigation of bacteria in Culicoides (University of Liverpool), modeling of mosquito populations (Reading) and development of novel insecticides and repellent formulations (LSHTM). These specialist expertise were driven by the host organizations for the studies.
Impact Products are still in development.
Start Year 2017
 
Description Suresh Kuchipudi and Janet Daly 
Organisation Penn State University
Country United States 
Sector Academic/University 
PI Contribution We have hosted a co-supervised PhD student twice at The Pirbright Institute. During these visits we have helped with viral infection of primary cells for analysis of PI3K pathway perturbations and generated new GM influenza viruses to help to understand if the difference in PI3K pathway activity observed between two different avian influenza viruses in avian cells was a result of difference in the NS1 protein. In addition we have provided support to the student regarding the future directions of the project and writing up and presentation of the material. Therefore we have provided reagents, practical expertise and intellectual input.
Collaborator Contribution The collaborators have provided access to data generated in their laboratories and intellectual input to the project.
Impact Publication: doi: 10.1038/srep17999
Start Year 2016
 
Description Suresh Kuchipudi and Janet Daly 
Organisation University of Nottingham
Department School of Veterinary Medicine and Science Nottingham
Country United Kingdom 
Sector Academic/University 
PI Contribution We have hosted a co-supervised PhD student twice at The Pirbright Institute. During these visits we have helped with viral infection of primary cells for analysis of PI3K pathway perturbations and generated new GM influenza viruses to help to understand if the difference in PI3K pathway activity observed between two different avian influenza viruses in avian cells was a result of difference in the NS1 protein. In addition we have provided support to the student regarding the future directions of the project and writing up and presentation of the material. Therefore we have provided reagents, practical expertise and intellectual input.
Collaborator Contribution The collaborators have provided access to data generated in their laboratories and intellectual input to the project.
Impact Publication: doi: 10.1038/srep17999
Start Year 2016
 
Description Swine haplotyping 
Organisation Gift Of Life
Country United States 
Sector Charity/Non Profit 
PI Contribution Prepared and shipped samples
Collaborator Contribution Sample analysis
Impact Data generation. Not multi-disciplinary
Start Year 2016
 
Description The GCRF One Health Poultry Hub 
Organisation Royal Veterinary College (RVC)
Country United Kingdom 
Sector Academic/University 
PI Contribution The focus of the research is to achieve sustainable global intensification of poultry meat and egg production whilst reducing risks to human and animal health and welfare. We are undertaking research to investigate the diversity of avian influenza viruses prevalent in poultry and wild birds in south east Asia.
Collaborator Contribution Royal Veterinary College, London is leading this GCRF One Health Poultry Hub. The project will address the need to meet rising demand for poultry meat and eggs in developing countries, while minimising risk to international public health. Population growth is driving global demand for poultry, meat and egg production; this unfortunately creates conditions in which animal diseases can spread to humans ('zoonoses'). These include bacterial food poisoning and strains with avian influenza with epidemic or pandemic potential. The GCRF One Health Poultry Hub will adopt a 'One Health' approach to the issue of combatting animal-to-human diseases by bringing together a team of laboratory, clinical, veterinary and social scientists. This team will test and evaluate novel interventions. The need for safe poultry production is most urgent in South and South East Asia, so the RVC and its partners will then use their local networks in these regions to put its positive research to immediate use.
Impact Development of international interdisciplinary partnership of 55 investigators from 13 countries to address the emerging global challenges of food security and public health associated with intensification of poultry production.
Start Year 2018
 
Description The Pirbright "Livestock Antibody Hub" funded by Bill & Melinda Gates Foundation 
Organisation The Pirbright Institute
Country United Kingdom 
Sector Academic/University 
PI Contribution Me and my team established collaboration in the "Livestock Antibody Hub" funded ($5.5 million) by the from Bill & Melinda Gates Foundation. Focus of our partnership is to develop techniques and capacity to analyse immune cells (B cells) of cattle, poultry and pigs that produce virus neutralizing antibodies. This research will support rational development of next generation of vaccines and to explore antibody-based passive immunization approaches for treatment and prophylaxis of infectious viral diseases affecting livestock and humans. Our initial focus is to target major animal viral pathogens that cause severe losses within Low and Middle-Income Countries (LMIC) animal production systems including avian influenza virus (AIV), Newcastle disease virus (NDV), infectious bursal disease virus (IBDV), infectious bronchitis virus (IBV) and foot-and-mouth disease virus (FMDV).
Collaborator Contribution This is a collaborative project and partners are providing help in provision of reagents and techniques for analysis of diversity of B cells repertoire that produce infleunza virus-specific antibodies, single cell sequencing and bioinformatic analysis. This project will further strengthen our on-going research collaborations with many research groups in academia as well as with animal health industry (Zoetis, Boehringer Ingelheim Animal Health, Merck, CEVA, Jovac, YeBio, ILRI, Galvmed, The Roslin Institute, HuMabs, AbCellera and Distributed Bio) to take the research outputs from laboratory to the field. The outputs of research will be improved vaccines and diagnostics enabling to reduce the impact of infectious diseases on farm animals, which offer substantial direct and indirect economic, public health, environmental and social benefits to the UK and rest of the world.
Impact Development of passive immunization approaches against avian influenza viruses affecting poultry (project incited in January 2020).
Start Year 2020
 
Description The Roslin Institute 
Organisation University of Edinburgh
Department The Roslin Institute
Country United Kingdom 
Sector Academic/University 
PI Contribution We have been working with several groups using our molecular and genetic data to help understand cattle cellular immunology. We have also hosted a visiting scientist to provide training in molecular techniques and data analysis, This has continued and now includes members of the Centre for Tropical Livestock Health and Genetics where we are engaged in sample sharing and maximising the value of genetic data by linking different sources as much as possible.
Collaborator Contribution Our partners have provided data and reagents as well as intellectual input to help us translate genetic diversity into functional diversity
Impact This collaboration is multi-disciplinary, although largely immunology and associated genetics is also involves bioinformatics
Start Year 2011
 
Description The effect of the microbiota on immunity to swine infleunza 
Organisation University of Surrey
Country United Kingdom 
Sector Academic/University 
PI Contribution We wish to establish the gut and nasal microbiome in normal healthy pigs and how this is affected by influenza infection. We have collected gut samples from pigs at different stages of infection, as well as nasal swabs which we will provide to our collaborators in Surrey.
Collaborator Contribution Our collaborators in Surrey will perform the sequencing and bioinformatics analysis to determine the microbial communities present in the samples.
Impact No outcomes yet - we are in the process of analysing the samples.
Start Year 2017
 
Description Thomas Bowden - STRUBI - University of Oxford - Glycoprotein structure collaboration 
Organisation University of Oxford
Country United Kingdom 
Sector Academic/University 
PI Contribution Provision of data and advice about the biological properties of viral glycoproteins, e.g. their fusion capacity with various receptors, their role in determining host-range
Collaborator Contribution Advice and technical support at the structural level supporting future project development and design
Impact No outcomes as yet
Start Year 2018
 
Description Trevor Sweeney - University of Cambridge PhD Studentship 
Organisation University of Cambridge
Country United Kingdom 
Sector Academic/University 
PI Contribution Development of PhD studentship project in collaboration with Dr. Sweeney's lab at Cambridge and application at Pirbright.
Collaborator Contribution Development of PhD studentship project and application at Cambridge.
Impact The PhD studentship has been funded and is currently been advertised.
Start Year 2018
 
Description University of Glasgow collaborations (Sam Wilson and Massimo Palmarini) 
Organisation University of Glasgow
Department MRC - University of Glasgow Centre for Virus Research
Country United Kingdom 
Sector Academic/University 
PI Contribution Scientific discussion and reagent sharing contributing to grant applications and active research projects
Collaborator Contribution Scientific discussion and reagent sharing contributing to grant applications and active research projects
Impact Successful MRC grant application
Start Year 2015
 
Description University of Liverpool Proteomics Collaboration 
Organisation University of Liverpool
Country United Kingdom 
Sector Academic/University 
PI Contribution Generation of samples to analyse the membranous proteome of cells infected with paramyxoviruses.
Collaborator Contribution Proteomic analysis of samples and technical support. Access to previous data-sets and expertise.
Impact Preliminary data sets only to date.
Start Year 2018
 
Description Wilhelm Gerner 
Organisation University of Veterinary Medicine Vienna
Country Austria 
Sector Academic/University 
PI Contribution Our lab hosted Dr Gerner who brought samples for RNA-seq. My lab planned and executed the sequencing and is contributing to the analysis.
Collaborator Contribution Dr Gerner did all the animal work and cell sorting to produce the samples reading for RNA-seq
Impact None as yet, analysis is ongoing
Start Year 2019
 
Description Y chromosome-linked X-shredding gene drives 
Organisation Hebrew University of Jerusalem
Country Israel 
Sector Academic/University 
PI Contribution We shared with the partner Y chromosome-linked docking lines generated in our lab.
Collaborator Contribution Provided some plasmid constructs.
Impact No outputs yet.
Start Year 2021
 
Description Talk Presented at 2nd International Congress of Veterinary Microbiology, 16th -19th October 2018. Sherwood Breezes Resort-Lara, Antalya, Turkey. 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Conference discussed improving prevention and control of viral diseases of poultry.
Year(s) Of Engagement Activity 2018
 
Description "Challenges for Poultry Industry". Organised by Fakieh Poultry at the Ritz-Carlton Hotel in Jeddah, Saudi Arabia, 2nd -3rd December 2018, 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Conference discussed prevention and control of infectious diseases affecting poultry production.
Year(s) Of Engagement Activity 2018
 
Description "Interactive session with poultry stakeholders". Title: Overview of Avian Influenza group Research at The Pirbright Institute. Presented at University of Veterinary and Animal Sciences (UVAS) Lahore, Pakistan, 3rd August 2018. 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact The workshop discussed the economic impacts and prevention strategies against avian influenza viruses affecting poultry production and zoonotic infections.
Year(s) Of Engagement Activity 2018
 
Description 1st Research Coordination Meeting (RCM) of the Coordinated Research Project (CRP) on "Generic approach for the development of genetic sexing strains for SIT applications" Vienna, 7-11 October 2019 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact FAO/IAEA Coordinated Research Project on exploring genetic and molecular methods of sex separation in insect pests; aimed at promoting specific areas of research, exchange of ideas and networking among the experts in the field.
Year(s) Of Engagement Activity 2019
 
Description 2. Overview of Avian Influenza group Research at The Pirbright Institute. Presented at "Interactive session with poultry stakeholders" at University of Veterinary and Animal Sciences (UVAS) Lahore, Pakistan, 3rd August 2018. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Dissemination of research Project outcomes These include presenting research data for vaccine seed strains with emergent field avian influenza variants; The zoonotic risk posed by emerging avian influenza viruses circulating in the wild birds and poultry. Impact of virus evolution on vaccine efficacy and persistence in poultry.
Year(s) Of Engagement Activity 2018
 
Description 360 degree Lab Animal Tour (and film) of The Plowright Building, The Philip Mellor Insectary, and Animal Isolation Facilities 
Form Of Engagement Activity Engagement focused website, blog or social media channel
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact The website provides unique access to the animal research facilities at The Pirbright Institute which support its world-leading scientific research into livestock viral diseases and helps save countless animal and potentially human lives, globally. Produced and filmed by Understanding Animal Research, the Lab Animal Tour was filmed at Pirbright, and at three other research centres, and provides the viewer with an interactive 360 virtual tour of the animal facilities.
Year(s) Of Engagement Activity 2017
URL http://www.labanimaltour.org/pirbright
 
Description 7th European Veterinary Immunology Workshop 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Selected oral presentation: Developing a 'One Health' Nipah virus vaccine to protect animal and public health
Year(s) Of Engagement Activity 2021
 
Description A talk at a symposium entitled: Using the 3Rs to support good science 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact I gave a talk at a Symposium held at The Pirbright Institute entitled: "Using the 3Rs to support good science". The talk was entitled: "A chicken primary B cell culture model to study the pathogenesis and improve the control of immunosuppressive viruses of poultry"
Year(s) Of Engagement Activity 2017
 
Description A talk at the British Poultry Diseases Group meeting 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact The Poultry Diseases Group meet quarterly and is comprised of representatives from private veterinary practices, vaccine and pharmaceutical industries, and the Animal and Plant Health Agency (APHA). I gave a talk at one of the meetings outlining my research to date and future directions.
Year(s) Of Engagement Activity 2017
 
Description AHSV talk on transmission for OIE (Thailand online) 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Talk on transmission of AHSV to interested parties following outbreak in 2020. Talk given on 9/6/20 to approx 150.
Year(s) Of Engagement Activity 2020
 
Description AN INTERVIEW WITH PROFESSOR VENUGOPAL NAIR 
Form Of Engagement Activity Engagement focused website, blog or social media channel
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Professor Venugopal Nair is a Research Group Leader at The Pirbright Institute, a visiting Professor of Avian Virology at the Department of Zoology, and a Jenner Investigator at the Jenner Institute, University of Oxford. He is also a member of the Microbiology Society, and in this interview, he tells us more about his research into viral diseases of livestock.
Year(s) Of Engagement Activity 2020
URL https://microbiologysociety.org/membership/meet-our-members/focus-area-viruses/an-interview-with-pro...
 
Description Amin Asfor presented the findigs of the project at the Microbiology Society Annual meeting, 2019 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Amin Asfor presented the findigs of the project at the Microbiology Society Annual meeting, 2019. Title: in vivo and ex vivo models of Infectious Bursal Disease Virus (IBDV) in inbred chicken lines differing in their resistance to the disease.
Year(s) Of Engagement Activity 2019
 
Description Amin Asfor presented the work at the NC3Rs Fellows Day 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Amin Asfor presented a poster at the NC3Rs Fellows Day entitled: Chicken primary B cell culture model to study the pathogenesis and improve the control of immunosuppressive viruses of poultry
Year(s) Of Engagement Activity 2019
 
Description Amy Lewis, The Scientist, 15/08/2017 re gene drive and Predator Free NZ 2050 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Media (as a channel to the public)
Results and Impact Provided information and quotes for article on potential use of gene drives as part of New Zealand effort to eradicate invasive predators
Year(s) Of Engagement Activity 2017
URL https://www.the-scientist.com/?articles.view/articleNo/50180/title/Driving-Down-Pests/
 
Description Ash Manor GCSE Mentoring 2018 - BA 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Stimulating increased interest in research
Year(s) Of Engagement Activity 2018
 
Description Ash Manor school challenge week science workshops 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Provide information on research activity at The Pirbright Institute and informing on pathogens infecting farmed animals.
Year(s) Of Engagement Activity 2019
 
Description Ash Manor school challenge week science workshops 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Discussion of research activities with school children and the school reported increased interests in the related subject.
Year(s) Of Engagement Activity 2019
 
Description Avian Influenza H9N2 and H7N9 evolution, fitness in poultry and zoonotic potential presented by Munir Iqbal at UC DAVIS EDUCATION CONFERENCE ON ONE HEALTH FOR FOOD SAFETY, AGRICULTURE, AND ANIMAL HEALTH 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact The research was discussed with academic and non-academic participants the risks of avian influenza viruses currently circulating in poultry and carry potential to cause zoonotic infections. Since avian influenzas viruses (AIVs) exist in many subtypes and co-infection of two or more different AIV subtypes in an individual bird can lead to the generation of novel reassortant viruses. The emergent reassortant viruses may carry differential phenotypic characteristics (virulence, host-transmission and host-range) compared to their parental viruses. Our studies revealed that experimental co-infection of chickens with two different subtypes of avian influenza viruses (H9N2 and H7N9) led to the emergence of novel reassortant H9N9 viruses which carry greater virulence for poultry and an increased zoonotic and pandemic potential.

Our results also provided evidence that both H9N2 and H7N9 viruses can rapidly acquire antigenic changes in vaccinated birds causing vaccine failure and cocirculation of these viruses can also rapidly lead to rapid generation of novel reassortment viruses with increased virulence and host-range posing threat to both animals and humans.

This presentation increased awareness among the audience risk posed by co-circulation of different subtypes of avian influenza viruses in poultry and wild birds and how best we can detect emerging threats and develop the disease risks and disease mitigation strategies.
Year(s) Of Engagement Activity 2021
URL https://www.wifss.ucdavis.edu/onehealthsummer21/
 
Description Avian influenza virus evolution impacts on virulence and antigenicity 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Postgraduate students
Results and Impact A talk was presented at Royal Veterinary College, London, by Thusitha Karunarathna (a PhD student in the Avian Influenza group at The Pirbright Institute). The Talk described the mechanisms used by the avian influenza viruses to evade host immunity to cause disease outbreaks and persists in poultry.
Year(s) Of Engagement Activity 2021
 
Description Avian influenza viruses can combine two techniques to evade vaccine immunity 
Form Of Engagement Activity Engagement focused website, blog or social media channel
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Media (as a channel to the public)
Results and Impact A study by researchers at The Pirbright Institute reveals more clues about how avian influenza viruses can evade vaccine immunity, aiding their spread in the field. The findings are published in Emerging Microbes and Infections.

Influenza viruses are able to mutate, which can enable them to evade immunity generated by natural infection or vaccination. Influenza viruses have four main ways of duping the immune system so that they can continue to infect and spread between birds. Pirbright scientists investigated two of these to further understand how the H9N2 avian influenza virus can change its genetic makeup to overcome poultry vaccines.

One of the methods the virus can use is to alter one of its surface proteins, haemagglutinin (HA), which enables the virus to enter the cell and replicate. HA is also a common target for the immune system's antibodies, which block the virus from binding to cell receptors. By changing just one or two protein components, HA can latch on far more tightly to the cell, preventing antibodies from stopping the virus. However, this can come at a cost, as particularly strong binding can prevent effective replication.

The other method scientists investigated is how H9N2 viruses disguise themselves by adding sugar chains to the surface of their HA proteins. This can block antibodies from binding, but also has varying fitness outcomes for the influenza virus. The researchers found that the location of the sugar chain on HA could determine how effectively the virus replicated.

They also demonstrated that the two evasive techniques could either compliment or act against each other - if an influenza virus had gained mutations that enable it to bind more strongly to cells, a sugar chain in the right position could restore its ability to replicate effectively. In contrast, weakly binding influenza viruses do not benefit from HA sugar additions, which instead reduce the virus's ability to replicate.

Professor Munir Iqbal, head of the Avian Influenza Group at Pirbright said, "We can use this knowledge to develop new vaccines that will help antibodies to recognise how the flu virus can change. This will provide protection to birds even as the virus evolves. We can also use this information to understand how viruses survive in poultry despite vaccination and monitor new adaptions that may appear in the field."

This research was funded by the Biotechnology and Biological Sciences Research Council (BBSRC), part of UK Research and Innovation (UKRI) and the British Council with support from the Newton Fund.

Article: Sealy, J. E., Peacock, T. P., Sadeyen, J. R., Chang, P., Everest, H. J., Bhat, S., Iqbal, M. (2020). Adsorptive mutation and N-linked glycosylation modulate influenza virus antigenicity and fitness. Emerging Microbes & Infections, advance online publication, 12 November 2020, doi: 10.1080/22221751.2020.1850180

[SOURCE: The Pirbright Institute]
Year(s) Of Engagement Activity 2020
URL https://www.star-idaz.net/2020/12/avian-influenza-viruses-can-combine-two-techniques-to-evade-vaccin...
 
Description Avian influenza viruses in Bangladesh, Vietnam, and Sri Lanka: investigating genotype to phenotype (antigenicity, virulence, host-range). Webinar on 28th March 2021. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Presented talk by Munir iqbal describing ongoing research outcomes. We identified genetic determined that modules avian influenza H5 and H9 antigenicity virulence and transmission fitness in different host species. The role of identified markers that change the virus binding to host cells and impact on virus-host interaction leading to mild or severe disease manifestation in chickens and mammalian species (humans).
Year(s) Of Engagement Activity 2021
 
Description Avian influenza: tackling large numbers of outbreaks this winter in the UK 
Form Of Engagement Activity A magazine, newsletter or online publication
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact Nearly two years on, the COVID-19 pandemic is still dominating the news and continues to cause concern for populations across the globe. However, UK poultry and wild bird populations are currently facing their own health threat. We are not the only ones that have faced lockdowns because of spreading viruses, UK poultry and captive birds have also faced a similar situation to protect them from avian influenza. Scientists at The Pirbright Institute are working to increase understanding of how genetic changes in influenza A viruses can impact disease spread, host responses to infection and the pathology associated with the disease.

What is the avian influenza virus?
Avian influenza viruses are Influenza A viruses that circulate and transmit amongst birds. The disease is what we commonly know as 'bird flu'. Influenza A viruses can infect a range of hosts, including humans and two subtypes H1N1 and H3N2 circulate as seasonal flu each winter in the UK. Avian influenza viruses are different to those that can infect humans in that they cannot easily infect humans and must change and adapt to do so.

Aquatic birds are a natural reservoir for avian influenza viruses, these birds typically experience fewer clinical symptoms but can spread disease to domestic birds, particularly in the migratory season, between October and March. Avian influenza viruses are categorised as either highly pathogenic (HPAI) or low pathogenic (LPAI) and this categorisation is linked to the severity of disease in chickens.

HPAI has a high mortality rate which can be up to 100%, other symptoms can include swelling of the head, death of cells in the comb and wattle, red discolouration of legs and feet and diarrhoea. HPAI causes disease in more organs than just those in the respiratory or gastrointestinal tracts like LPAI, which mostly presents as respiratory symptoms such as a snick (like a sneeze), a rattle in the chest (known as rales) and discharge from the eyes and nose.

What is causing bird flu outbreaks in domestic birds now?
We are currently in the normal migratory season of wild birds. In the spring and summer months, these birds spend their time breeding and raising their young in North-eastern Asia where they mix with other bird species which enables virus transmission and mutation. Then, when they migrate for the autumn and winter months, they shed virus in their faeces along the way.

One of the migratory paths is across Northern Europe and this includes the UK. This year, in the UK, the first avian influenza outbreak was on 15 October and was discovered in captive aquatic birds. To date (17 December 2021) there have been 50 outbreaks in England, 1 in Wales and 2 in Scotland, this has resulted in over half a million birds being destroyed to control the spread of disease.

Are there more outbreaks this year compared to other years?
The number of outbreaks this year has been the most extensive ever. There have been over 50 outbreaks in the UK, compared to 26 outbreaks last year. Although it is hard to pin down the reason for this high number, we know it is not linked to the COVID-19 pandemic. The UK's response to the spread of avian influenza has been rapid, and from 29 November birds were required to be housed inside with additional biosecurity measures put in place such as foot dips and disinfecting clothing.

Testing and surveillance of wild birds have shown that there is a high prevalence of the virus in these birds currently, and this is contributing to the spread of disease to farm and domestic bird populations.

A more detailed look at the genetic make-up of these viruses shows that they are different to those from previous years. This is predicted to be as a result of the virus infecting multiple birds across a range of species, and this provides the perfect opportunity for the virus to evolve. Analysis of these genetic changes does not raise any alarm bells for increased transmission to humans, but they are likely impacting the spread and severity of disease in birds.

What should we do if we find a dead bird or are concerned about bird health?

If the public finds dead waterfowl birds they are encouraged not to touch them but to report them to Defra using the appropriate phone number that can be found on the website, also any other wild birds found dead in numbers of five or greater should be reported.

What is Pirbright doing to tackle this virus?

Researchers at Pirbright are attempting to enhance the control and detection of avian influenza in poultry populations as well as extend our basic knowledge about the virus in avian and mammalian hosts. The research groups led by Professor Munir Iqbal and Dr Holy Shelton are investigating how avian influenza virus strains originated from wild birds can rapidly adapt to cause disease outbreaks in poultry and gain the potential to infect humans. In addition, Professor Munir Iqbal is developing improved poultry vaccines that prevent virus replication and spread. These vaccines are particularly useful in countries where avian influenza is regularly found in the domestic poultry and the control and prevention of spread isn't easy, such as in the UK.
Year(s) Of Engagement Activity 2021
URL https://www.pirbright.ac.uk/news/2021/12/avian-influenza-tackling-large-numbers-outbreaks-winter-uk
 
Description BBC Today programme 24/01/2019 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Media (as a channel to the public)
Results and Impact Interview on BBC Today programme regarding potential development and use of gene drives in mice
Year(s) Of Engagement Activity 2019
URL https://www.bbc.co.uk/sounds/play/m00025bt
 
Description BBSRC COP Climate Change Bites 21/05/2021 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Postgraduate students
Results and Impact BBSRC-sponsored event "Climate Change Bites" - talks and panel discussion about the effects of climate change on vector-borne disease
Year(s) Of Engagement Activity 2021
URL https://bbsrc.ukri.org/news/events/2021/climate-change-events/
 
Description BBSRC Partnering Seminar, University of Liverpool, Tamilnadu Veterinary and Animal Science University 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Talk presented entitled " Molecular determinants of antigenicity of H7 and H9 avian Influenza viruses". The research data shared with the colleagues working to reduce the impact of avian infectious diseases on poultry production. The discussion led to more collaboration and work together, acquiring reagents and protocols for further research and development activities.
Year(s) Of Engagement Activity 2019
 
Description BBSRC website article: Halloween 
Form Of Engagement Activity Engagement focused website, blog or social media channel
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact Article on BBSRC website: Halloween Creepy Crawlies
Year(s) Of Engagement Activity 2017
URL http://www.bbsrc.ac.uk/news/fundamental-bioscience/2017/171031-f-uk-creepiest-creepy-crawlies/
 
Description BIotech YES 2019 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Postgraduate students
Results and Impact Gave a talk at the BBSRC Biotechnology Young Entrepreneurs' Scheme event at Syngenta (Jealott's Hill)
Year(s) Of Engagement Activity 2019
 
Description BSI report on the future of veterinary immunology and vaccinology 
Form Of Engagement Activity A magazine, newsletter or online publication
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Other audiences
Results and Impact Organised and published a joint IVVN/BSI report to raise awareness of the UK's research status in veterinary vaccinology and immunology and the importance of maintaining this for the R&D landscape 'Securing Our Future: the value of veterinary vaccines'. This was aimed at influencing policymakers to support and commit to maintaining the UK's leading position.
Year(s) Of Engagement Activity 2021
 
Description BSI webinar 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact I presented a webinar hosted by the British Society for Immunology entitled "BSI Coronavirus webinar: What can we learn from the animal coronaviruses?", which sparked questions and discussions afterwards. I have since been contacted to establish new collaborations with people in the audience.
Year(s) Of Engagement Activity 2020
 
Description Big Band Science Event- AH, ER, LVC, MdP 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Stimulating increased interest in science and research
Year(s) Of Engagement Activity 2019
 
Description Big Bang Fair 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact This is a South of England Showground in which students from schools and their parents participated which lead to discussion and interest in both school children and their parents.
Year(s) Of Engagement Activity 2019
URL https://nearme.thebigbangfair.co.uk/view/?eve_id=1956
 
Description Big Bang Fair 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Schools
Results and Impact The Big Bang Near Me is a programme of regional and local Big Bang Fairs that take place all across the UK. Together with The Big Bang UK Young Scientists & Engineers Fair and The Big Bang Competition, it forms part of the wider Big Bang programme, bringing science, technology, engineering and maths (STEM) to life for young people
Year(s) Of Engagement Activity 2019
URL https://nearme.thebigbangfair.co.uk/about/
 
Description Big Bang Fair South East - RC 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Regional science festival/event - changes in public attitudes and stimulating increased interest in research
Year(s) Of Engagement Activity 2018
 
Description Big Bang UK- Jennifer Simpson 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Schools
Results and Impact Big Bang UK is a science competition. JS acted as moderator for final projects submitted by students from across the UK.
Year(s) Of Engagement Activity Pre-2006,2019
 
Description Biotech YES, Syngenta Jealott's Hill, 05 Oct 2017 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Postgraduate students
Results and Impact Teams of postgraduate students from a number of universities and institutes attended talk as part of Biotech YES (Young Entrepreneurs' Scheme). Follow-up discussion influenced several aspects of their approach to entrepreneurship both within the competition and more generally
Year(s) Of Engagement Activity 2014,2015,2016,2017
 
Description Bird flu study from Pirbright Institute sheds light on mutations and health impacts 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Media (as a channel to the public)
Results and Impact Bird flu study from Pirbright Institute sheds light on mutations and health impacts. Emerging research from the Pirbright Institute shows that avian influenza mutations could increase disease threat to poultry, but make it less infectious for humans.

Mutations in the H7N9 bird flu virus increase its replication rate and stability in avian cells, but reduce its preference for infecting human cells, a study from The Pirbright Institute has found. The results show that outbreaks in birds caused by strains with these mutations could pose a lower risk to human health, while remaining a significant threat to the poultry industry.

H7N9 low-pathogenicity avian influenza (LPAI) virus usually infects birds, but in 2013 the first human case was recorded in China. Since then, there have been over 1,500 confirmed human infections with about 40 percent of cases resulting in fatalities. Further evolution of the H7N9 virus gave rise to a high-pathogenicity avian influenza (HPAI) strain that could cause 100 percent mortality in chickens. In 2017 the Chinese Government initiated a wide scale vaccination programme in chickens which drove down cases of both low and high pathogenicity strains.

Previous Pirbright research identified three mutations in avian influenza H7N9 viruses that enabled them to overcome immunity generated by vaccines. The mutations altered a protein on the outside of the virus called haemagglutinin (HA), which binds to host cell receptors and allows the virus to enter and cause infection.

In the latest study, published in the Journal of Virology, the team discovered the same mutations occurred in H7N9 viruses isolated from the field in 2019, which most likely emerged in birds that had either been immunised or naturally infected. Analysis of the viruses demonstrated that strains with these mutations have significantly increased replication rates in both chicken cell cultures and chick embryos as well as displaying greater acid and thermal stability, which could improve transmission ability.

The mutations also influenced which animal cells the strain could enter - their HA proteins could still bind to the receptors of bird cells, but they lost their affinity for human cell receptors. These findings show that in evolving to escape the chicken immune system, the mutated H7N9 viruses have reduced their risk to human health whilst increasing the threat they pose to poultry, although further studies in birds are needed to confirm the impact this could have on infection and disease spread.

Professor Munir Iqbal, head of the Avian Influenza Group at Pirbright, said: "Our approach has allowed us to accurately predict avian influenza mutations that appear in the field, and assess how these mutations could affect the risk they pose to both human and avian populations. This information can be used to inform surveillance efforts and provide early warnings of potentially dangerous emerging strains."
Year(s) Of Engagement Activity 2020
URL https://www.thepoultrysite.com/news/2020/07/bird-flu-study-from-pirbright-institute-sheds-light-on-m...
 
Description Blenheim High School Fair - DB 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Team members attended sessions at a local high school with secondary school children and those in the 6th form to talk about paths into scientific careers, sharing their experiences of working at The Pirbright Institute. Approximately 300 students attended and it stimulated increased interest in science and research.
Year(s) Of Engagement Activity 2019
 
Description Bluetongue and Wildlife (15 minute presentation on Epizootic Haemorhagic Disease Virus), (July/Pirbright, UK) 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Policymakers/politicians
Results and Impact Policymakers reported greater interest in deer-associated diseases including EHDV and BTV
Year(s) Of Engagement Activity 2018
 
Description Briefing - EU/Estonia parliamentarians and policy advisers 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Policymakers/politicians
Results and Impact Briefed Estonian delegation to Brussels/EU parliament on gene drives and related issues
Year(s) Of Engagement Activity 2020
 
Description Brownies-AH 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Talking to the Brownies about science and their careers
Year(s) Of Engagement Activity 2019
 
Description CABI_Research activity of Avian Immunology Group at The Pirbright Institute 
Form Of Engagement Activity Engagement focused website, blog or social media channel
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Media (as a channel to the public)
Results and Impact Release of research activity within Avian Immunology group at The Pirbright Institute
Year(s) Of Engagement Activity 2018
 
Description CBD Open-ended online forum on synbio 06-10/2017 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Policymakers/politicians
Results and Impact UN Convention on Biodiversity. Policy- discussion on synthetic biology: nominated participant in the Open Ended Online Forum on Synthetic biology as established in decision XII/24 of the Conference of the Parties to the Convention on Biological Diversity (COP) and extended in COP decision XIII/17 which ran from June to Oct 2017 (approx.)
Year(s) Of Engagement Activity 2017
URL http://bch.cbd.int/synbio/open-ended/discussion/
 
Description CBS briefing 26/05/2021 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Media (as a channel to the public)
Results and Impact Briefed CBS journalist in relation to story about release of transgenic mosquitoes in Florida
Year(s) Of Engagement Activity 2021
 
Description CNN interview 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Media (as a channel to the public)
Results and Impact Interview with CNN journalist. Journalist later followed up asking me to check and clarify some issues.
Year(s) Of Engagement Activity 2019
 
Description Cafe Scientifique, Bradfield School, West Berkshire: "How insects spread viruses" 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact Cafe Scientifique is a public forum for exploring the latest ideas in science and technology. Meetings take place in cafes, bars, restaurants and even theatres, but always outside a traditional academic context. I presented to an audience of the general public and answered questions.
Year(s) Of Engagement Activity 2017
 
Description Cafe Scientifique, Reading: "How insects spread viruses" 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact Cafe Scientifique is a public forum for exploring the latest ideas in science and technology. Meetings take place in cafes, bars, restaurants and even theatres, but always outside a traditional academic context. I presented to an audience of the general public and answered questions.
Year(s) Of Engagement Activity 2007,2017
 
Description Camberley Library Fun Palace 2018 - JM 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact Changes in public attitudes and stimulating increased interest in research
Year(s) Of Engagement Activity 2018
 
Description Caroline Seydel Genetic Literacy Project re GM mosquitoes/gene drive/Zika 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Media (as a channel to the public)
Results and Impact Provided background material and quotes for article on GM mosquitoes and control of Zika virus.
Year(s) Of Engagement Activity 2017
URL https://geneticliteracyproject.org/2018/01/29/waging-war-mosquito-split-gene-drive-hinder-spread-zik...
 
Description Centre of excellence for research on avian diseases (CERAD) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact I was invited to present my work at the UK-China Centre of excellence for research on avian diseases (CERAD) meeting attended by researchers from UK, China and Thailand. There was lots of discussion about the research and future directions, including potential collaborations.
Year(s) Of Engagement Activity 2017
 
Description Chair and Co-Organiser for UK Vector-borne Diseases Conference 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact UK Vector-borne Diseases meeting held bi-Annually. Half day sessions on 2nd, 9th, 16th, 23rd November 2020. Chaired session on 9th November 2020. Meeting was held online with invited and selected presentations and a poster session. Also included funders from Defra and BBSRC.
Year(s) Of Engagement Activity 2020
 
Description Cheltenham Science Festival 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Institute stand at the festival covering the Institute science in general, and transmission and gene editing to control disease, in particular.
Year(s) Of Engagement Activity 2018
 
Description Cheltenham Science Festival 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Stimulating increased interest in science and research
Year(s) Of Engagement Activity 2019
 
Description Cheltenham Science Festival 2018 - JA 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Major science festival - changes in public attitudes and stimulating increased interest in research
Year(s) Of Engagement Activity 2018
 
Description Cheltenham Science Festival 2018 - JM 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Major science festival - changes in public attitudes and stimulating increased interest in research
Year(s) Of Engagement Activity 2018
 
Description Cheltenham Science Festival 2018 - JP 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Major national science festival - changes in public attitudes and stimulating increased interest in research
Year(s) Of Engagement Activity 2018
 
Description Cheltenham Science Festival 2018 - RC 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Major science festival - changes in public attitudes and stimulating increased interest in research
Year(s) Of Engagement Activity 2018
 
Description Cheltenham Science Festival 2018 - SV 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Display at major science festival: changes in public attitudes and stimulating increased interest in research
Year(s) Of Engagement Activity 2018
 
Description Cheltenham Science Festival 2018-DP 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Major national science festival - changes in public attitudes and stimulating increased interest in research
Year(s) Of Engagement Activity 2018
 
Description Cheltenham Science Festival- AH, ER, JC 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Present at the Pirbright stand to discuss science with the public
Year(s) Of Engagement Activity 2019
 
Description Cheltenham Science festival - LB 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact National science festival which Team members attended to demonstrate to the public how scientists at The Pirbright Institute can work to contain and control viral outbreaks. Over 500 people accessed the information and this stimulated increased interest in science and research.
Year(s) Of Engagement Activity 2019
 
Description Cheltenhan Science Festival - Dana Perry 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact DP helped to run a stand at the festival, questions from the general public stimulated increased interest in science and research and increased the visibility of The Pirbright Institute.
Year(s) Of Engagement Activity 2019
 
Description Combating avian influenza through systematic analysis of antigenic drift, genetic variation, and development of novel diagnostic tools and vaccines. Presented at as PI of the ZELS project at ZELS grant holders and Stakeholders meeting in Hanoi, Vietnam 23th-24th January 2017. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Engagement and dissemination of research outcome to representatives of research funding bodies such as Department for International Development (DFID), Medical Research Council (MRC) and the researchers working on different research projects funded by BBSRC under Zoonoses and Emerging Livestock Systems (ZELS).
Year(s) Of Engagement Activity 2018
 
Description Commentary on AHSV outbreak in Thailand in Science Magazine 
Form Of Engagement Activity A magazine, newsletter or online publication
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Media (as a channel to the public)
Results and Impact 'Thailand scrambles to contain major outbreak of horse-killing virus' - Article in Science Mag April 2020
Year(s) Of Engagement Activity 2020
URL https://www.sciencemag.org/news/2020/04/thailand-scrambles-contain-major-outbreak-horse-killing-viru...
 
Description Conference session organiser at MMC2017 - Host Pathogen Interactions 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Chair for the Imaging Host-Pathogen Interactions session run at MMC2017 (and in previous years). Session usually attracts approx. 30 delegates from national/international backgrounds, mostly academic. Talks sparked discussions and networking opportunities.
Year(s) Of Engagement Activity 2012,2014,2015,2017
 
Description Coronavirus lecture MSc students (Surrey) 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Postgraduate students
Results and Impact A lecture was presented on the replication, cellular interactions and pathogenesis of coronaviruses to 15-20 MSc students from University of Surrey. The students were engaged and interested, answering and asking questions during the session.
Year(s) Of Engagement Activity 2016,2018
 
Description DARPA visit April 2017 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Supporters
Results and Impact Hosted visit by US funding agency programme manager to The Pirbright Institute
Year(s) Of Engagement Activity 2017
 
Description DB Winston Churchill School Careers event 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact School careers event for a Secondary School - 1500 children ages 11 to 16 years
Year(s) Of Engagement Activity 2021
 
Description Deimante Lukosaityte: Microbiology Society conference 2019. Poster presentation: Chicken protection against H9N2 virus by passive immunization 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Described techniques for development of next generation of vaccines against viral diseases affecting animals and humans.
Year(s) Of Engagement Activity 2019
 
Description Demonstrating Mathematics (Angita Shrestha) 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Postgraduate students
Results and Impact Angita Shrestha (PhD student within Avian Influenza group) provided help in a Maths session to the new Oxford Doctoral Training Porgramme (DTP) cohort.
Year(s) Of Engagement Activity 2018
 
Description Determinants of antigenicity of H9 Avian Influenza Viruses. UK-China Swine and Poultry Workshop.17-18 June 2019, The Pirbright Institute, UK. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact The talk and the discussion on the improvement of disease control systems (vaccines and diagnostics) to prevent avian influenza viruses infection in poultry and zoonotic infections in humans.
Year(s) Of Engagement Activity 2019
 
Description Diamond (TT) 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Diamond Light Source Open Day - explaining to general public the importance of structural biology and microscopy for understanding viruses and designing improved vaccines.
Year(s) Of Engagement Activity 2019
 
Description Diamond Light Source Open day - HJM 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact A public open day for Diamond Light Source, which Pirbright attended as a user of the facility to showcase research being performed at Pirbgith in collaboration with Diamond. There was a lot of interest in the fundamental research and sicussion about future plans and how it fit with the facility at Diamond.
Year(s) Of Engagement Activity 2019
 
Description Diamond open day 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Over 300 people visited our stand at the open day, in which we described the importance of our work for food security and public health. Children from all ages were involved and learned about viruses and played with the huge virus particle that we had produced. One of the major impact was that we described to several people why we work on some viruses that we do not have them in the UK, and the importance of the work for the UK and UK economy and public health was explained.
Year(s) Of Engagement Activity 2017
URL http://www.diamond.ac.uk/Public/VisitUs.html
 
Description Diego Friere Pesquisa Fapesp 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Media (as a channel to the public)
Results and Impact Interview with journalist leading to article regarding release of transgenic mosquitoes into the environment to control dengue and other diseases
Year(s) Of Engagement Activity 2019
URL https://revistapesquisa.fapesp.br/en/2020/02/05/jacobina-controversy/
 
Description Discuss gene drives with NZ Parliamentary Commissioner 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Policymakers/politicians
Results and Impact Met NZ Parliamentary Commissioner to provide background information on, and discuss, gene drive systems. New Zealand is considering genetic and other control methods for invasive pests.
Year(s) Of Engagement Activity 2018
 
Description EFSA Hearing Expert Gene Drive Environmental Risk Assessment Working Group 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Policymakers/politicians
Results and Impact Hearing Expert for EFSA Gene Drive Environmental Risk Assessment Working Group. Contributed to EFSA Scientific Opinion.
Year(s) Of Engagement Activity 2020
URL https://efsa.onlinelibrary.wiley.com/doi/full/10.2903/j.efsa.2020.6297
 
Description EFSA consultation July 2020 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Policymakers/politicians
Results and Impact Responded to European Food Standards Authority (EFSA) request for input regarding regulation of gene drives (public consultation)
Year(s) Of Engagement Activity 2020
URL https://efsa.onlinelibrary.wiley.com/doi/abs/10.2903/sp.efsa.2020.EN-1939
 
Description EM-UK 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Co-organiser and speaker for UKRI supported network for electron microscopists in the UK. I was one (or two) of the founder members. Meeting has been running for 5 years. Increased the visibility of The Pirbright Institute.
Year(s) Of Engagement Activity 2020
 
Description EM-UK: A national network for electron microscopists 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Founder and Chair of EM-UK, a national network (with international participants) for electron microscopists. Includes a one or two day meeting with invited speakers, and email discussion list for questions, comments, advertising courses, advertising jobs. It has proved very popular with over 200 people registering for the discussion list, and 90+ delegates at each meeting. Administration is handled by the Royal Microscopical Society.
Year(s) Of Engagement Activity 2016,2017,2018
 
Description EMBO Workshop: Molecular and population biology of mosquitoes and other disease vectors: vector and disease control 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Presentation on "Sex determination pathway in Anopheles gambiae as a target for mosquito control" given during the meeting held on 22 - 26 July 2019 in Kolymbari, Greece. Following a presentation a request was made to give an informal seminar at the Department of Life Sciences, Imperial College, London.
Year(s) Of Engagement Activity 2019
URL https://meetings.embo.org/event/19-mosquitoes
 
Description EPO Inventors Revisited video 
Form Of Engagement Activity A broadcast e.g. TV/radio/film/podcast (other than news/press)
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact Podcast for European Patent Office "Inventors Revisited" series
Year(s) Of Engagement Activity 2020
URL https://www.epo.org/news-events/events/european-inventor/inventors-revisited.html
 
Description EPO TalkInnovation Facebook live event and podcast 
Form Of Engagement Activity A broadcast e.g. TV/radio/film/podcast (other than news/press)
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact EPO TalkInnovation Facebook live event and podcast discussing use of genetic methods to control mosquitoes and mosquito-borne disease
Year(s) Of Engagement Activity 2020
URL https://www.facebook.com/europeanpatentoffice/videos/a-minute-change-that-makes-a-massive-difference...
 
Description EU parliament webinar 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Policymakers/politicians
Results and Impact Invited speaker for European Parliament Intergroup on Climate Change, Biodiversity and Sustainable Development webinar "Research and Innovation for biodiversity: what role for gene drive research?"
Year(s) Of Engagement Activity 2020
URL https://ebcd.org/event/online-event-research-and-innovation-for-biodiversity-what-role-for-gene-driv...
 
Description Edinburgh Science Festival 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Talk and panel event at Edinburgh Science Festival "The Buzz about GM Insects". Sparked questions and discussions afterwards including some career questions.
Year(s) Of Engagement Activity 2019
 
Description Edinburgh seminar 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Postgraduate students
Results and Impact Gave invited seminar at Edinburgh University (via Zoom) regarding development and applications of transgenic mosquitoes.
Year(s) Of Engagement Activity 2021
 
Description Elle Campbell Presented her data at the 2019 Microbiology Society Annual Meeting 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Elle Campbell gave an oral presentation at the 2019 Microbiology Society Annual Meeting on the work we were doing with fluorescently tagged IBDVs and virus factory movement.
Year(s) Of Engagement Activity 2019
 
Description Encouraging women into science and engineering STEM. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact MH Visited Warwick School, Redhill to talk to secondary students about careers in STEM. A very positive outcome and well received.
Year(s) Of Engagement Activity 2018
 
Description Engineered Recombinant Single Chain Variable Fragment of Monoclonal Antibody Provides Protection to Chickens Infected with H9N2 Avian Influenza. Presented by Deimante Lukosaityte at Influenza Update meeting 4th - 15th December 2020: The University of Liverpool (online meeting) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact The talk describes a technique for the development of immunotherapy to protect poultry from Infectious diseases such as avian influenza viruses. The talk provided evidence that recombinant antibodies can provide passive immunity to protect chickens from avian influenza. Several mAbs generated from mice hybridomas targeting hemagglutinin (HA) of H9N2 virus were converted into single-chain variable fragment (scFv) antibodies. Recombinant scFvs were purified from insect cell culture supernatants and showed it could retain neutralization capacity against H9N2 virus in vitro. To test therapeutic scFv efficacy in vivo we selected two of the recombinantly produced scFv antibodies for prophylaxis and treatment of chickens infected with H9N2 UDL-01 virus. Intranasally treated birds showed reduced disease manifestation and virus shedding. These results indicate that antibody therapy could be beneficial for the provision of rapid treatment for reducing disease impacts in poultry.
Year(s) Of Engagement Activity 2020
URL https://www.gla.ac.uk/researchinstitutes/iii/cvr/events/influenza/
 
Description European Society Vector Ecology (Invited Chair for two sessions and speaker for 15min talk); (October/Palermo, Sicily) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Chaired two sessions, one on vector-borne diseases, one on scientific networks. Latter included a discussion with early career researchers. Main outcome was coordination between active networks including The Gnatwork.
Year(s) Of Engagement Activity 2018
URL http://www.sove.org/European%20SOVE%20folder/greecescientificprogram/palermo_Scientific_program_revi...
 
Description Evolution of H9N2 avian influenza virus under immune pressure. Presented at 10th International Symposium on Avian Influenza, 15th - 18th April 2018, The Grand Hotel, Brighton, UK. 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact The primary aim of the workshop was to forge long-term research partnerships between early-career researchers, livestock industry and national disease control authorities in the UK and the Philippines. In addition, the activity hopes provide information about the emerging and next generation diagnostic and vaccine strategies and their utilization to reduce the impact of viral diseases on livestock and poultry.
Year(s) Of Engagement Activity 2019
URL https://www.slideshare.net/zerep_cire/2019-newton-agham-researcher-links-workshop-vaccines-and-diagn...
 
Description Ewen Callaway Nature 08/01/2018 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Media (as a channel to the public)
Results and Impact Provided expert background information for article in Nature regarding engineering species barriers, described by journalist as "very helpful" in followup email.
Year(s) Of Engagement Activity 2018
 
Description Expert commentary on New Scientist article 
Form Of Engagement Activity A magazine, newsletter or online publication
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact I provided expert commentary on an article published in New Scientist, which summarised research performed by researchers at the University of Leeds on how mosquito bites enhance the transmission of vector-borne diseases.
Year(s) Of Engagement Activity 2020
URL https://institutions.newscientist.com/article/2231141-skin-cream-applied-to-mosquito-bites-stops-vir...
 
Description FAO/IAEA Coordinated Research Programme on methods of sex separation in mosquitoes; Research coordination meeting Bangkok 19-23 February 2018 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact FAO/IAEA Coordinated Research Programme on exploring genetic, molecular, mechanical, and behavioral methods of sex separation in mosquitoes; aimed at promoting specific areas of research, exchange of ideas and networking among the experts in the field.
Year(s) Of Engagement Activity 2013,2015,2016,2018
 
Description FLI - presentation 
Form Of Engagement Activity A talk or presentation
Part Of Official