Science Services
Lead Research Organisation:
The Pirbright Institute
Department Name: UNLISTED
Abstract
Within the area of Science Services we have identified five areas that are vital for the efficient running of science for both internal and external users of materials and facilities. These are (1) Insectary Support; (2) Bioimaging; (3) Immunological Resources Support; (4) Sequencing and Bioinformatics; (5) Proteomics. These are national capabilities either due to the specialised and costly containment infrastructure required for their running and maintenance or that they have been built up as a result of long-term strategic funding and heavy use by both internal and external users.
The Science Services National Capability has been used to address COVID-19 related challenges from 1 April 2020.
The Science Services National Capability has been used to address COVID-19 related challenges from 1 April 2020.
Organisations
- The Pirbright Institute, United Kingdom (Lead Research Organisation)
- Institute of Zoology (Collaboration)
- University of Edinburgh, United Kingdom (Collaboration)
- Huvepharma (Collaboration)
- Charles University, Czech Republic (Collaboration)
- AIMM Therapeutics (Collaboration)
- University of Dundee, United Kingdom (Collaboration)
- Helmholtz Association of German Research Centres (Collaboration)
- Animal and Plant Health Agency (Collaboration)
- Bauchi State University (Collaboration)
- Liverpool John Moores University, United Kingdom (Collaboration)
- Harvard University (Collaboration)
- University of St Andrews, United Kingdom (Collaboration)
- University of Cambridge (Collaboration)
- University of Liverpool, United Kingdom (Collaboration)
- University of Pennsylvania, United States (Collaboration)
- National Center for Genetic Engineering and Biotechnology (BIOTEC) (Collaboration)
- Friedrich Loeffler Institute (Collaboration)
- Sisaf Ltd, QUEENS ROAD (Collaboration)
- National Taiwan University, Taiwan, Province of China (Collaboration)
- University of Minnesota, United States (Collaboration)
- University of Oxford, United Kingdom (Collaboration)
- MSD Animal Health, United Kingdom (Collaboration)
- Chinese Academy of Agricultural Sciences (Collaboration)
- VUC Aarhus (Collaboration)
- Hebrew University of Jerusalem (Collaboration)
- APS Biocontrol (Collaboration)
- University of Bristol, United Kingdom (Collaboration)
- London Sch of Hygiene and Trop Medicine, United Kingdom (Collaboration)
- Government of Thailand (Collaboration)
- Thermo Fisher Scientific (Collaboration)
- Technical University of Denmark (Collaboration)
- National Health Research Institutes (NHRI) Taiwan (Collaboration)
- University of Greenwich, United Kingdom (Collaboration)
- National Cheng Kung University, Taiwan, Province of China (Collaboration)
- University of Copenhagen, Denmark (Collaboration)
- The Pirbright Institute, WOKING (Collaboration)
- National Institute of Genetics (Collaboration)
- University of Sussex, United Kingdom (Collaboration)
- Texas A&M University-Central Texas (Collaboration)
- Polytechnic University of Valencia (Collaboration)
- Eco Animal Health Ltd (Collaboration)
- Washington State University, United States (Collaboration)
- Lancaster University (Collaboration)
- Biomathematics and Statistics Scotland (BioSS) (Collaboration)
- Syngenta International AG (Collaboration)
- Swedish University of Agricultural Sci, Sweden (Collaboration)
- Kansas State University, United States (Collaboration)
- University of Lleida, Spain (Collaboration)
- National Institute for Biological Standards and Control (NIBSC) (Collaboration)
- The Royal Microscopical Society (Collaboration)
- Rothamsted Research, United Kingdom (Collaboration)
- Centre for Genomic Regulation (CRG) (Collaboration)
- Enesi Pharma (Collaboration)
- University of Veterinary Medicine Hanover (Collaboration)
- University of Lyon (Collaboration)
- Rosalind Franklin Institute (Collaboration)
- University of Parma, Italy (Collaboration)
- Wageningen University & Research (Collaboration)
- Unlisted (Collaboration)
- University of Kent, United Kingdom (Collaboration)
- Aviagen Group (Collaboration)
- Swiss Federal Institute of Technology (ETH), Zurich (Collaboration)
- University of Queensland, Australia (Collaboration)
- Sanofi (Collaboration)
- Bern University of Applied Sciences (Collaboration)
- Zoological Soc London Inst of Zoology, United Kingdom (Collaboration)
- University of Miyazaki (Collaboration)
- Imperial College London, United Kingdom (Collaboration)
- Queen's University of Belfast, United Kingdom (Collaboration)
- State Central Veterinary Laboratory (Collaboration)
- Medical Research Council (Collaboration)
- University of York, United Kingdom (Collaboration)
- University of Leeds, United Kingdom (Collaboration)
- University of Surrey, United Kingdom (Collaboration)
- University of Veterinary Medicine Vienna (Collaboration)
- Royal Veterinary College, United Kingdom (Collaboration)
- Leidos (Collaboration)
Publications
Edmans M
(2020)
Magnitude and Kinetics of T Cell and Antibody Responses During H1N1pdm09 Infection in Inbred Babraham Pigs and Outbred Pigs.
in Frontiers in immunology
Vatzia E
(2021)
Respiratory and Intramuscular Immunization With ChAdOx2-NPM1-NA Induces Distinct Immune Responses in H1N1pdm09 Pre-Exposed Pigs.
in Frontiers in immunology
Paudyal B
(2021)
Low Dose Pig Anti-Influenza Virus Monoclonal Antibodies Reduce Lung Pathology but Do Not Prevent Virus Shedding.
in Frontiers in immunology
Entrican G
(2020)
The Veterinary Immunological Toolbox: Past, Present, and Future
in Frontiers in Immunology
Edmans MD
(2021)
Identification and Phenotype of MAIT Cells in Cattle and Their Response to Bacterial Infections.
in Frontiers in immunology
Xu J
(2018)
Antiviral Immunotoxin Against Bovine herpesvirus-1: Targeted Inhibition of Viral Replication and Apoptosis of Infected Cell.
in Frontiers in microbiology
Rodriguez L
(2018)
Identification of Amino Acid Residues Responsible for Inhibition of Host Gene Expression by Influenza A H9N2 NS1 Targeting of CPSF30.
in Frontiers in microbiology
Brown E
(2021)
Characterising Foot-and-Mouth Disease Virus in Clinical Samples Using Nanopore Sequencing.
in Frontiers in veterinary science
Steyn A
(2020)
The Characterization of chIFITMs in Avian Coronavirus Infection In Vivo, Ex Vivo and In Vitro.
in Genes
| Title | E17 Art Trail - STEAM theme (Jennifer Simpson) |
| Description | Laboratory of Tiny Things installation which ran in London for three weeks. Open to the general public. |
| Type Of Art | Image |
| Year Produced | 2017 |
| Impact | No known impact |
| Title | The Pathologist - Through the Looking Glass (Jennifer Simpson) |
| Description | Article showcasing a gallery of beautiful images from all walks of laboratory medicine |
| Type Of Art | Image |
| Year Produced | 2017 |
| Impact | No impacts known |
| Description | The Science Services strategic grant provides unique resources to scientists both within and external to Pirbright. During the reporting period to date we have 1) Supplied insects to a variety of external users under 25 extant Material Transfer Agreements. As a National Capability, we primarily supply unique lines of mosquitoes, biting midges and large biting flies free of charge to academic users that cannot otherwise access these resources. In addition, industrial income has been met as estimated within the impact plan. Staffing has also been strengthened towards lab management of the two main insectary facilities in line with requirements of users and areas requiring containment level 3 techniques have also been strengthened by the addition of a dedicated lab manager. 2) Bioimaging facilities for internal users have been maintained with more than 50 registered users and a range of outputs in line with impact plan estimates derived from both microscopy and flow cytometry techniques. Workers in this area have retained direct links to relevant societies and provided a range of materials for outreach activities. In addition, a new strategic collaboration has been established with the Electron Bioimaging Centre at Diamond Light Source and a bid for a new Transmission Electron Microscope has been successful to facilitate this work (2019). 3) Sequencing of hybridoma collections has been initiated (>50 to date) and a committee formed for selection and distribution of resources that includes national and international experts (2017). This is aimed at providing immunological resources that are not commercially viable but nonetheless a limiting factor in studies of host and virus. Outputs include a dedicated website that is accessible to the public and enables searching and ordering of available reagents (2019). 4) In addition to the current MiSeq facility, a HiSeq Illumina sequencer has been installed (2018) and is running in containment and single cell encapsulation technology is in preliminary trials (2019). A total of 14 research groups have accessed these facilities to date (2019). 5) Proteomics support has been provided to at least seven internal groups and has also been assessed across site with a significant increase in demand. |
| Exploitation Route | Facilities within Science Services are provided across the groups at Pirbright and every group leader has received at least some provision of support within the five distinct areas. We have targeted insect supplies and the immunological toolbox as being the most straightforward to provide to external users and both of these areas have made resources available that have enabled users to carry out science that would otherwise not be possible. Both of these areas rely on centralizing both expertise and resources which would not be normally be supported at a single institution, improving both continuity and critical mass and are within the range of reporting identified within the impact plan. Bioimaging and sequencing in containment operate on a slightly different model of external access due to the constraint of the training required to access facilities, but plans are in place to improve the numbers accessing resources later in the ISPG programmes. The proteomics service is a purely internal service operated in collaboration with the University of Liverpool. |
| Sectors | Agriculture, Food and Drink |
| Description | The provision of Science Services underpins a vast range of scientific discoveries funded by our two institute strategic programme grants and uplift funding outlined in the publications produced to date. These impacts would not be achieved without the supporting services described, facilitating a diverse range of areas. Major areas include the use of DNA/RNA sequencing in containment where samples cannot be outsourced for this service and the production of both insect and animal lines that are unique to Pirbright under the national capability initiative. In addition, the development of bioimaging in containment has led to discussion with other groups active in the field in the UK and the formation of a working group around developing techniques. The production of insect and vertebrate hosts for viruses also forms a major aspect of our aim to carry out studies of virus infection in the natural hosts in addition to providing opportunities for methods to vaccinate or protect from infection. |
| First Year Of Impact | 2017 |
| Sector | Agriculture, Food and Drink |
| Impact Types | Societal,Economic,Policy & public services |
| Description | ALERT20 Co-Chair |
| Geographic Reach | National |
| Policy Influence Type | Participation in a advisory committee |
| Impact | Provision of equipment to enhance and enable fundamental research within BBSRC remit |
| Description | BBSRC 18ALERT panel member |
| Geographic Reach | National |
| Policy Influence Type | Participation in a advisory committee |
| Impact | Provision of multi-user equipment to core facilities to enable BBSRC remit science. Part of the remit for this call was to allow shared access from other institutions, widening the impact of BBSRC investment. |
| Description | BBSRC ALERT19 panel member (invited) |
| Geographic Reach | National |
| Policy Influence Type | Participation in a advisory committee |
| Impact | Panel for ALERT18 multi-user equipment award by BBSRC |
| Description | BBSRC Bioimaging Review |
| Geographic Reach | National |
| Policy Influence Type | Participation in a advisory committee |
| Description | BBSRC-Newton Fund Agricultural Data Re-use Advisory Group |
| Geographic Reach | National |
| Policy Influence Type | Participation in a advisory committee |
| Impact | Advice on re-usability of data generated in research projects. |
| Description | Bioimaging Steering Group |
| Geographic Reach | Local/Municipal/Regional |
| Policy Influence Type | Membership of a guideline committee |
| Impact | Better management of workload and facility internally |
| Description | Bioimaging Steering Group |
| Geographic Reach | Local/Municipal/Regional |
| Policy Influence Type | Membership of a guideline committee |
| Impact | Improving access and efficient organization of Bioimaging for researchers at Pirbright |
| Description | Contribution to a POSTnote on 'Reducing UK Antibiotic Use in Animals' |
| Geographic Reach | National |
| Policy Influence Type | Participation in a advisory committee |
| Description | Data on SARS-COV-2 antigenicity for government committees, e.g. UKHSA variant technical group, NERVtag |
| Geographic Reach | National |
| Policy Influence Type | Implementation circular/rapid advice/letter to e.g. Ministry of Health |
| Impact | This information was routinely fed into UKHSA and government decision making on managing the Covid-19 pandemic |
| Description | Grants Review Team |
| Geographic Reach | Local/Municipal/Regional |
| Policy Influence Type | Membership of a guideline committee |
| Impact | More effective inclusion of microscopy technology in grants submitted from the Institute |
| Description | IUCN Assessment of Synthetic Biology and Biodiversity Conservation |
| Geographic Reach | Multiple continents/international |
| Policy Influence Type | Participation in a advisory committee |
| URL | https://www.iucn.org/theme/science-and-economics/our-work/other-work/synthetic-biology-and-biodivers... |
| Description | Inter-institute imaging network |
| Geographic Reach | National |
| Policy Influence Type | Participation in a advisory committee |
| Description | International Society for Neglected Tropical Diseases Dengue Advisory Group |
| Geographic Reach | Multiple continents/international |
| Policy Influence Type | Participation in a advisory committee |
| URL | https://www.isntd.org/dengue-advisory-group |
| Description | Invited member of RCaH Science Advisory Board |
| Geographic Reach | National |
| Policy Influence Type | Participation in a advisory committee |
| Impact | The Research Complex at Harwell is a UKRI funded building with the remit of enabling researchers across many disciplines to take advantage of the national facilities on the Harwell campus. RCaH is going through significant change at the moment and the advisory board has been asked to contribute to defining its future direction. |
| Description | Lumpy Skin Disease Control Strategy for Great Britain |
| Geographic Reach | National |
| Policy Influence Type | Participation in a advisory committee |
| Description | MSc research training |
| Geographic Reach | Multiple continents/international |
| Policy Influence Type | Influenced training of practitioners or researchers |
| Impact | The immediate outcome of this impact activity is that these Masters students went on to the following subsequent career destinations: PhD programme: 2 students Technical position in research institution: 1 student |
| Description | Member of RMS EM Section committee |
| Geographic Reach | Multiple continents/international |
| Policy Influence Type | Membership of a guideline committee |
| Impact | This committee informs the RMS on which areas to invest funds in regarding training, conferences, outreach events. RMS members are international. |
| Description | Membership of MPEG-G (standardisation of the compression of genomic information) working group |
| Geographic Reach | Multiple continents/international |
| Policy Influence Type | Membership of a guideline committee |
| Description | Microbiology Society Members Panel |
| Geographic Reach | Multiple continents/international |
| Policy Influence Type | Participation in a advisory committee |
| URL | https://microbiologysociety.org/why-microbiology-matters/council-governance/standing-panels/members-... |
| Description | Organised seminar series on Sequencing, Virus Evolution and Molecular Biology at Pirbright (with Luca Ferretti and Graham Freimanis) |
| Geographic Reach | Local/Municipal/Regional |
| Policy Influence Type | Influenced training of practitioners or researchers |
| Description | Oxford Brookes Bioimaging Steering Group |
| Geographic Reach | Local/Municipal/Regional |
| Policy Influence Type | Participation in a advisory committee |
| Description | POSTnote Report on Climate and vector-diseases in humans in the UK |
| Geographic Reach | National |
| Policy Influence Type | Gave evidence to a government review |
| Description | PhD research training |
| Geographic Reach | Multiple continents/international |
| Policy Influence Type | Influenced training of practitioners or researchers |
| Impact | The concrete outcomes of this PhD training was the skills learnt by the respective students while under my guidance. Their immediate destinations after graduating were: Postdoctoral academic research (US): one student Postdoctoral academic research (UK): one student |
| Description | Publication policy document for core facilities - EM-UK |
| Geographic Reach | Multiple continents/international |
| Policy Influence Type | Influenced training of practitioners or researchers |
| Impact | I am a founder member of the network created for electron microscopists within UK, EM-UK. The annual meeting is highly productive, and training and managing core facilities is regularly discussed. Recognition of these facilities is essential for their continued existence so we produced a generic document hosted on the RMS website which can be adopted by any core facility in any institution, and describes the publication policy which users had to adhere to. This has already raised the profile of core facilities around the country and is a successful outcome from the EM-UK meetings. |
| Description | Royal Microscopical Society Microscopy Training Focussed Interest Group |
| Geographic Reach | Multiple continents/international |
| Policy Influence Type | Influenced training of practitioners or researchers |
| Description | Royal Microscopical Society Training Focussed Interest Group |
| Geographic Reach | Multiple continents/international |
| Policy Influence Type | Influenced training of practitioners or researchers |
| Impact | A review of the microscopy training courses available globally has been undertaken. We are in the process of collating and preparing a gap analysis. This will be used to guide RMS policy on training provided in the UK, both UK and international delegates. |
| Description | Scientific Advisory Group for Emergencies Exercise (Royal Society: 11/06/19): Examined simulated emergence of arbovirus in the UK with main decision making bodies and personnel. |
| Geographic Reach | National |
| Policy Influence Type | Gave evidence to a government review |
| Description | Training MSc students |
| Geographic Reach | Local/Municipal/Regional |
| Policy Influence Type | Influenced training of practitioners or researchers |
| Impact | Delivered a new training session to MSc students teaching viral quantification assays. This lead to a greater understanding of laboratory techniques and academic research. |
| Description | University of Surrey MSc Vet microbiology - teaching (Jennifer Simpson) |
| Geographic Reach | Local/Municipal/Regional |
| Policy Influence Type | Influenced training of practitioners or researchers |
| Impact | Two days of teaching practical microscopy, a useful cross disciplinary skill for many researchers |
| Description | (CloudButton) - Serverless Data Analytics Platform |
| Amount | € 4,277,507 (EUR) |
| Funding ID | 825184 |
| Organisation | European Commission |
| Sector | Public |
| Country | European Union (EU) |
| Start | 01/2019 |
| End | 12/2021 |
| Description | A One Health Approach to Alkhumra Haemorrhagic Fever Virus Species Tropism, Pathogenesis and Epidemiology |
| Amount | £99,082 (GBP) |
| Organisation | The Pirbright Institute |
| Sector | Academic/University |
| Country | United Kingdom |
| Start | 09/2022 |
| End | 04/2026 |
| Description | A One Health approach to pan-valent morbillivirus vaccines |
| Amount | £773,237 (GBP) |
| Funding ID | BB/R019843/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 04/2018 |
| End | 03/2021 |
| Description | An immunogenetic approach to guide the need for booster shots and combat immune failure in SARS-CoV-2 vaccine response |
| Amount | £987,464 (GBP) |
| Funding ID | MR/W020629/1 |
| Organisation | Medical Research Council (MRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 07/2021 |
| End | 08/2022 |
| Description | Antigenic characterisation of infectious bursal disease virus to improve vaccination strategies and vaccine design |
| Amount | £450,685 (GBP) |
| Funding ID | BB/S014594/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 06/2019 |
| End | 12/2021 |
| Description | Assessing the functionality of avian BST2 as a viral restriction factor. |
| Amount | £50,000 (GBP) |
| Organisation | University of Oxford |
| Sector | Academic/University |
| Country | United Kingdom |
| Start | 09/2021 |
| End | 09/2025 |
| Description | Assessment of the immunogenicity of bovine herpesvirus 4-based vectors delivering Nipah virus glycoproteins in swine |
| Amount | £41,895 (GBP) |
| Organisation | European Commission |
| Sector | Public |
| Country | European Union (EU) |
| Start | 02/2019 |
| End | 04/2019 |
| Description | Aviagen Industry Funding |
| Amount | £422,383 (GBP) |
| Organisation | Aviagen Group |
| Sector | Private |
| Country | United States |
| Start | 08/2019 |
| End | 12/2020 |
| Description | BBSRC IAA |
| Amount | £25,000 (GBP) |
| Funding ID | BB/S506680/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 08/2021 |
| End | 03/2022 |
| Description | BBSRC IAA The Pirbright Institute |
| Amount | £300,000 (GBP) |
| Funding ID | BB/S506680/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 03/2018 |
| End | 03/2021 |
| Description | BBSRC Industrial CASE (iCASE) studentship programme Oxford Interdisciplinary Bioscience Doctoral Training Partnership |
| Amount | £80,000 (GBP) |
| Organisation | University of Oxford |
| Sector | Academic/University |
| Country | United Kingdom |
| Start | 09/2019 |
| End | 09/2023 |
| Description | BBSRC business case for sequencing capacity in containment. |
| Amount | £560,000 (GBP) |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 11/2017 |
| End | 03/2018 |
| Description | BBSRC responsive mode grant |
| Amount | £367,000 (GBP) |
| Funding ID | BB/P006841/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 09/2017 |
| End | 03/2019 |
| Description | Biotechnology and Biological Sciences Research Council (BBSRC) and University of Oxford joint Doctoral Training Project |
| Amount | £100,000 (GBP) |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 08/2018 |
| End | 08/2022 |
| Description | British Egg Marketing Board PhD studentship |
| Amount | £76,000 (GBP) |
| Organisation | British Egg Marketing Board |
| Sector | Private |
| Country | United Kingdom |
| Start | 09/2018 |
| End | 09/2022 |
| Description | Broad and effective protection against influenza achieved by viral vectored vaccines |
| Amount | £1,607,691 (GBP) |
| Funding ID | MR/S037160/1 |
| Organisation | Medical Research Council (MRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 04/2019 |
| End | 04/2022 |
| Description | Capital Infrastructure - PIrbright |
| Amount | £23,533 (GBP) |
| Organisation | The Pirbright Institute |
| Sector | Academic/University |
| Country | United Kingdom |
| Start | 12/2017 |
| End | 12/2017 |
| Description | Commonwealth Scholarship. Molecular determinants impacting avian influenza H9N2 virus evolution, replication fitness and virulence |
| Amount | £200,000 (GBP) |
| Funding ID | LKCS-2019- 665 |
| Organisation | British Council |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 11/2019 |
| End | 10/2022 |
| Description | Components of the Medea gene drive system in a mosquito Aedes aegypti |
| Amount | £1,209,348 (GBP) |
| Funding ID | BB/S001964/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 11/2018 |
| End | 10/2021 |
| Description | DARPA PREEMPT |
| Amount | $2,700,000 (USD) |
| Organisation | Defense Advanced Research Projects Agency (DARPA) |
| Sector | Public |
| Country | United States |
| Start | 09/2018 |
| End | 03/2022 |
| Description | Defining the circadian clock in chicken cells and its impact upon viral replication |
| Amount | £4,500 (GBP) |
| Organisation | The Houghton Trust |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 11/2019 |
| End | 11/2020 |
| Description | Defining the molecular signature of pericyte dysfunction in severe dengue patients |
| Amount | $20,000 (USD) |
| Organisation | UGPN |
| Sector | Academic/University |
| Start | 09/2020 |
| End | 07/2021 |
| Description | Dengue haemorrhage: a novel role for perivascular cells |
| Amount | £74,137 (GBP) |
| Organisation | University of Surrey |
| Sector | Academic/University |
| Country | United Kingdom |
| Start | 09/2021 |
| End | 09/2024 |
| Description | Development of next generation vaccine technology inducing rapid and strong immunity through targeted delivery of antigens to chicken immune cells |
| Amount | £473,080 (GBP) |
| Funding ID | BB/T013087/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 05/2020 |
| End | 05/2023 |
| Description | Development of therapeutic monoclonal antibodies for coronaviruses |
| Amount | £120,000 (GBP) |
| Organisation | The Pirbright Institute |
| Sector | Academic/University |
| Country | United Kingdom |
| Start | 09/2022 |
| End | 09/2026 |
| Description | ERA-NET Cofund on International Coordination of Research on Infectious Animal Diseases (ICRAD) |
| Amount | £1,526,000 (GBP) |
| Funding ID | SE1518 |
| Organisation | Department For Environment, Food And Rural Affairs (DEFRA) |
| Sector | Public |
| Country | United Kingdom |
| Start | 03/2021 |
| End | 03/2024 |
| Description | EU VetBioNet Transnational Access Fund: Evaluation of the immunogenicity of a bivalent PRRS-Nipah virus vaccine |
| Amount | £61,000 (GBP) |
| Organisation | European Commission |
| Sector | Public |
| Country | European Union (EU) |
| Start | 04/2021 |
| End | 07/2021 |
| Description | Enhancing protective efficacy of avian influenza vaccines through targeted delivery of protective antigens to chicken immune cells |
| Amount | £98,212 (GBP) |
| Funding ID | BB/R50595X/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 08/2017 |
| End | 09/2021 |
| Description | Floid Cell imager (X2) |
| Amount | £24,459 (GBP) |
| Organisation | The Pirbright Institute |
| Sector | Academic/University |
| Country | United Kingdom |
| Start | 11/2021 |
| End | 03/2022 |
| Description | G2P-UK; A National Virology Consortium to address phenotypic consequences of SARSCoV-2 genomic variation |
| Amount | £2,497,230 (GBP) |
| Funding ID | MR/W005611/1 |
| Organisation | Medical Research Council (MRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 02/2021 |
| End | 07/2022 |
| Description | Genomia Project Grant |
| Amount | £174,052 (GBP) |
| Organisation | Genomia fund |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 02/2019 |
| End | 01/2020 |
| Description | Global characterisation of interlinked mosquito immune networks |
| Amount | £9,000 (GBP) |
| Organisation | The Pirbright Institute |
| Sector | Academic/University |
| Country | United Kingdom |
| Start | 07/2021 |
| End | 03/2022 |
| Description | H2020 work programme topic 9 Food security, sustainable agriculture and forestry, marine and maritime and inland water research and the bioeconomy, work topic SFS-10-2017 Research and approaches for emerging diseases in plants and terrestrial livestock |
| Amount | € 5,986,250 (EUR) |
| Organisation | European Commission H2020 |
| Sector | Public |
| Country | Belgium |
| Start | 04/2018 |
| End | 04/2023 |
| Description | How does the lung protect itself against influenza? |
| Amount | £120,000 (GBP) |
| Organisation | The Pirbright Institute |
| Sector | Academic/University |
| Country | United Kingdom |
| Start | 06/2022 |
| End | 07/2026 |
| Description | Identification and evaluation of swine mAbs in pig influenza challenge model |
| Amount | $599,944 (USD) |
| Funding ID | OPP1201470 |
| Organisation | Bill and Melinda Gates Foundation |
| Sector | Charity/Non Profit |
| Country | United States |
| Start | 09/2018 |
| End | 10/2019 |
| Description | In vitro evaluation of peptide-based immune checkpoint inhibitors as potential molecular adjuvants for improved PRRS vaccines |
| Amount | £4,921 (GBP) |
| Organisation | The Pirbright Institute |
| Sector | Academic/University |
| Country | United Kingdom |
| Start | 07/2019 |
| End | 03/2020 |
| Description | Market research and technical feasibility of a bivalent porcine reproductive and respiratory syndrome / Nipah virus vaccine |
| Amount | £10,880 (GBP) |
| Organisation | The Pirbright Institute |
| Sector | Academic/University |
| Country | United Kingdom |
| Start | 06/2019 |
| End | 03/2020 |
| Description | NC3Rs Research Project Grant |
| Amount | £436,339 (GBP) |
| Funding ID | NC/R001138/1 |
| Organisation | National Centre for the Replacement, Refinement and Reduction of Animals in Research (NC3Rs) |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 12/2017 |
| End | 11/2019 |
| Description | OIE Twinning: Development of capacity for independent assessment of FMDV vaccines in Africa |
| Amount | $1,466,186 (USD) |
| Organisation | World Organisation for Animal Health |
| Sector | Public |
| Country | France |
| Start | 03/2019 |
| End | 03/2021 |
| Description | Oxford DTP - Diamond Light Source/Harwell |
| Amount | £100,000 (GBP) |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 09/2018 |
| End | 09/2022 |
| Description | PRCV model to better understand immunity to SARS-CoV2 |
| Amount | £50,000 (GBP) |
| Organisation | The Pirbright Institute |
| Sector | Academic/University |
| Country | United Kingdom |
| Start | 08/2021 |
| End | 03/2022 |
| Description | Pirbright Institute Flexible Talent Mobility Account |
| Amount | £180,000 (GBP) |
| Funding ID | BB/S507945/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 11/2018 |
| End | 03/2021 |
| Description | Pirbright Institute PhD studentship |
| Amount | £100,000 (GBP) |
| Organisation | The Pirbright Institute |
| Sector | Academic/University |
| Country | United Kingdom |
| Start | 08/2018 |
| End | 08/2022 |
| Description | Pirbright Institute PhD studentship |
| Amount | £100,000 (GBP) |
| Organisation | The Pirbright Institute |
| Sector | Academic/University |
| Country | United Kingdom |
| Start | 09/2019 |
| End | 09/2023 |
| Description | Pirbright Institute PhD studentship |
| Amount | £100,000 (GBP) |
| Organisation | The Pirbright Institute |
| Sector | Academic/University |
| Country | United Kingdom |
| Start | 09/2018 |
| End | 09/2022 |
| Description | Protecting poultry from avian influenza, Newcastle disease, infectious bronchitis, and Gumboro disease with a single dose of a multivalent vaccine |
| Amount | £448,937 (GBP) |
| Funding ID | BB/W003325/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 01/2022 |
| End | 12/2023 |
| Description | Recombinant pseudorabies virus as a multivalent vectored vaccine platform for emerging and endemic porcine diseases |
| Amount | £80,000 (GBP) |
| Organisation | The Pirbright Institute |
| Sector | Academic/University |
| Country | United Kingdom |
| Start | 09/2020 |
| End | 09/2024 |
| Description | Reducing the Economic and Zoonotic Impact of Avian Influenza (REZIAI): delivering novel vaccines and diagnostics from laboratory to the field. |
| Amount | £400,000 (GBP) |
| Funding ID | BB/S013792/1 |
| Organisation | The Pirbright Institute |
| Sector | Academic/University |
| Country | United Kingdom |
| Start | 02/2019 |
| End | 01/2021 |
| Description | SFS-10-2017 - Research and approaches for emerging diseases and pests in plants and terrestrial livestock: Addressing the dual emerging threats of African Swine Fever and Lumpy Skin Disease in Europe (DEFEND) |
| Amount | € 5,986,250 (EUR) |
| Funding ID | 773701 |
| Organisation | European Commission H2020 |
| Sector | Public |
| Country | Belgium |
| Start | 05/2018 |
| End | 05/2023 |
| Description | Seed Catalyst Award |
| Amount | £25,527 (GBP) |
| Funding ID | ISCF-TFPSA-Pirbright |
| Organisation | The Pirbright Institute |
| Sector | Academic/University |
| Country | United Kingdom |
| Start | 11/2018 |
| End | 02/2019 |
| Description | Smart biomimetic nanomaterials for novel treatment and prevention of viral infection |
| Amount | £60,000 (GBP) |
| Organisation | European Commission |
| Sector | Public |
| Country | European Union (EU) |
| Start | 03/2019 |
| End | 03/2022 |
| Description | The Global Challenges Research Fund |
| Amount | £640,473 (GBP) |
| Organisation | Research Councils UK (RCUK) |
| Sector | Public |
| Country | United Kingdom |
| Start | 03/2019 |
| End | 03/2024 |
| Description | The Pirbright Institute - University of Surrey PhD studentship |
| Amount | £100,000 (GBP) |
| Organisation | The Pirbright Institute |
| Sector | Academic/University |
| Country | United Kingdom |
| Start | 09/2019 |
| End | 03/2023 |
| Description | The Pirbright Institute - University of Surrey collaborative studentship: Not all viruses come alone - the impact of orbivirus co-infections on bluetongue virus reassortment and diversity |
| Amount | £100,000 (GBP) |
| Organisation | The Pirbright Institute |
| Sector | Academic/University |
| Country | United Kingdom |
| Start | 09/2021 |
| End | 03/2025 |
| Description | The Pirbright Livestock Antibody Hub |
| Amount | $5,530,900 (USD) |
| Funding ID | INV-003007 |
| Organisation | Bill and Melinda Gates Foundation |
| Sector | Charity/Non Profit |
| Country | United States |
| Start | 09/2019 |
| End | 09/2024 |
| Description | Towards a novel multivalent poultry vaccine: Development of a recombinant infectious bursal disease vectored vaccine encoding conserved B- and T- cell epitopes from infectious bronchitis virus |
| Amount | £5,000 (GBP) |
| Organisation | The Houghton Trust |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 11/2019 |
| End | 11/2020 |
| Description | Turnover of porcine lung tissue resident memory cells |
| Amount | £25,000 (GBP) |
| Organisation | The Pirbright Institute |
| Sector | Academic/University |
| Country | United Kingdom |
| Start | 08/2021 |
| End | 03/2022 |
| Description | Workpackage within a Horzion 20:20 grant |
| Amount | € 6,000,000 (EUR) |
| Funding ID | EU project 727393 Horizon 20:20 PALE-Blue |
| Organisation | European Union |
| Sector | Public |
| Country | European Union (EU) |
| Start | 05/2017 |
| End | 11/2021 |
| Description | [YY-EEID US-UK XXXX] Evaluating how immunosuppression influences influenza A virus transmission and evolution in wild and domestic birds |
| Amount | £606,360 (GBP) |
| Funding ID | BB/T008806/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 05/2019 |
| End | 06/2023 |
| Description | iCASE studentship: Production and assessment of antiviral prophylactic properties of natural biomolecules against avian influenza and Newcastle disease viruses affecting poultry |
| Amount | £100,000 (GBP) |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 09/2019 |
| End | 09/2023 |
| Title | A ligation and restriction enzyme independent cloning technique: an alternative to conventional methods for cloning hard-to-clone gene segments in the influenza reverse genetics system |
| Description | we developed an easy and efficient ligation and restriction enzyme independent (LREI) cloning method for cloning influenza gene segments into pHW2000 vector. The method involves amplification of megaprimers followed by PCR amplification of megaprimers using a bait plasmid, DpnI digestion and transformation. ligation and restriction enzyme independent (LREI) cloning method represents an alternative strategy for cloning influenza gene segments that have internal restriction sites for the enzymes used in reverse genetics. Further, the problem of genetic instability in bacteria can be alleviated by growing recombinant bacterial cultures at a lower temperature. This technique can be applied to clone any influenza gene segment using universal primers, which would help in the rapid generation of influenza viruses and facilitate influenza research and vaccine development. |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2020 |
| Provided To Others? | Yes |
| Impact | The method will help in the rapid generation of reverse genetic base influenza viruses and facilitate influenza research and vaccine development. |
| URL | https://virologyj.biomedcentral.com/articles/10.1186/s12985-020-01358-2 |
| Title | A male-specific cell line from a mosquito Anopheles gambiae |
| Description | A new male-specific cell line from a mosquito Anopheles gambiae has been isolated from sexed neonate larvae. To our knowledge this is the only cell line derived exclusively from male mosquitoes. |
| Type Of Material | Cell line |
| Year Produced | 2019 |
| Provided To Others? | No |
| Impact | The cell line will greatly facilitate studies on components of the sex determination pathway and of dosage compensation machinery in mosquitoes. There are only several cell lines derived from Anopheles gambiae available and these are female-like. Over-expression of genes involved in male sex determination or dosage compensation is detrimental to female cells, hence use of male-derived cells is necessary for such studies. The cell line will become available upon acceptance of a manuscript describing characterization of that cell line. |
| Title | An ex vivo IBDV infection model using a chicken primary B cell culture system |
| Description | Recently, it has become possible to culture chicken primary B cells in vitro in the presence of a soluble construct of chicken CD40L that was made at The Pirbright Institute. We have demonstrated that these chicken primary B cells can be infected with infectious bursal disease virus (IBDV). This system will be validated and expanded to other B cell tropic viruses in order to replace the use of infected chickens in research, in an NC3Rs funded grant. |
| Type Of Material | Model of mechanisms or symptoms - in vitro |
| Year Produced | 2017 |
| Provided To Others? | Yes |
| Impact | Using this system, we have determined that chicken primary B cells respond to IBDV infection by inducing Type I IFN responses. However, the induction is more pronounced in cells infected with an attenuated strain compared to a very virulent strain. This is consistent with the very virulent strain down-regulating antiviral responses to a greater extent than other strains which may, in part, explain its enhanced virulence. We also observed a reduction in the expression of key genes involved in B cell proliferation and activation following IBDV infection which was only possible by using the primary B cell culture system. |
| URL | https://www.ncbi.nlm.nih.gov/pubmed/?term=broadbent+dulwich |
| Title | Appropriate FMD vaccine strains for East Africa and South East Asia and new adjuvants for FMD vaccines that provide longer duration immunity |
| Description | We have identifies appropriate Vaccine strains for Serotyoe O and A for East Africa and for South east Asia which are published now. Additionally we have identified potent and safe adjuvants which has been tested in cattle and provides longer duration immunity. |
| Type Of Material | Improvements to research infrastructure |
| Year Produced | 2014 |
| Provided To Others? | Yes |
| Impact | Governments of East Africa and Regional OIE centre at South East Asia and vaccine producers are aware about the strains and adjuvants. |
| Title | CRISPR/Cas9-based gene editing of avian herpesvirus vectors to develop novel recombinant vaccines |
| Description | Development of recombinant herpesvirus vaccines are usually achieved by classical homologous recombination methods. The advent of new genome editing tools has enabled its application on avian herpesvirus vaccine strains to develop new recombinant vaccines potentially capable of expressing protective antigens from multiple pathogens |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2017 |
| Provided To Others? | Yes |
| Impact | The major impact will be in the generation of novel vaccines that can simultaneously protect against multiple avian diseases |
| URL | http://www.poultryworld.net/Health/Articles/2016/12/Is-GM-technology-the-future-of-poultry-vaccines-... |
| Title | Cattle MHC genotyping |
| Description | Using the sequence data generated through the targeted pull down of MHC, we developed a full gene and more targeted PCR approach to genotype cattle for the MHC class I region. This has been applied to many hundreds of samples to enable us to select individuals for breeding as well as survey genetic diversity in beef and diary herds. |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2018 |
| Provided To Others? | No |
| Impact | After publication which we anticipate in 2019, we will appy this method to targeted herds and are already attracting industry interest. |
| Title | Deep sequencing to identify RNA packaging signals |
| Description | A novel and simple approach to identify predicted RNA secondary structures involved in genome packaging in positive sense RNA viruses (e.g. the picornavirus foot-and-mouth disease virus [FMDV]). By interrogating deep sequencing data generated from both packaged and unpackaged populations of RNA, we have determined multiple regions of the genome with constrained variation in the packaged population. Predicted secondary structures of these regions revealed stem-loops with conservation of structure and a common motif at the loop. Disruption of these features resulted in attenuation of virus growth in cell culture due to a reduction in assembly of mature virions. This study provides evidence for the involvement of predicted RNA structures in picornavirus packaging and offers a readily transferable methodology for identifying packaging requirements in many other viruses. |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2017 |
| Provided To Others? | Yes |
| Impact | Impact so far is fundamental knowledge but with several potential avenues for future impact in for example improved vaccines. |
| Title | Detection of HA antigens of avian influenza viruses |
| Description | Assay for detection of recombinant HVT expressing HA antigens of avian influenza viruses |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2018 |
| Provided To Others? | Yes |
| Impact | This new method will help to detect recombinant HVT vaccine expressing HA antigen in infected cell. |
| Title | Development of SARS-CoV-2 pseudotypes |
| Description | Method to allow assessment of SARS-CoV-2 entry, which can be used in various experiments including VNTs to assess humoral immune response (in convalescent or vaccinated individuals) |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2020 |
| Provided To Others? | Yes |
| Impact | Application in the establishment of the SARS-CoV-2 international reference standard by NIBSC |
| Title | Development of assays for quantifying fusion inhibitory antibodies in sera (mFIT assay) |
| Description | We have developed a micro-fusion inhibition test (mFIT) assay to identify and quantify antibody responses (to vaccines etc.) which are capable of inhibiting viral induced cell-cell fusion. This test has been developed for various negative strand viruses (Nipah and RSV) as well as SARS-CoV-2 |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2020 |
| Provided To Others? | Yes |
| Impact | This assay was used to aide development of the NIBSC WHO international standard for SARS-CoV-2 |
| Title | Development of high-throughput assay for examining viral mediated cell-cell fusion |
| Description | We have developed and optimised high-throughput assays (96 well plate format) to examine viral induced cell-cell fusion, using an adapted Bi-FC approach. We are using this to identify inhibitors of paramyxovirus, e.g. measles and RSV, fusion and attachment proteins as well as SARS-CoV-2. This has been integrated with next-generation platforms such as the Incucyte real-time imaging system available at The Pirbright Institute, and the system has been rationalised so that it is entirely inducible. |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2018 |
| Provided To Others? | No |
| Impact | We are using this assay to examine the innate immune restriction of paramyxovirus replication. |
| Title | Fluorescence Recovery after Photobleaching (FRAP) of infectious bursal disease virus (IBDV) replication factories |
| Description | Fluorescence Recovery after Photobleaching (FRAP) of infectious bursal disease virus (IBDV) replication factories. |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2020 |
| Provided To Others? | No |
| Impact | We have used this technology to determine that IBDV replication factories display qualities of liquid-liquid phase separation |
| Title | IBDV Reverse Genetics System |
| Description | We have developed a reverse genetics system for IBDV. |
| Type Of Material | Technology assay or reagent |
| Provided To Others? | No |
| Impact | We have used the reverse genetics system to generate recombinant IBDVs and chimeric recombinant IBDVs that contain genes from classical and very virulent strains of IBDV in the background of a cell culture adapted attenuated strain. This will enable us to study the effect of individual virus genes on pathogenicity. |
| Title | Method for sequencing of ASFV (African Swine Fever Virus) based on long reads |
| Description | The ASFV genome is repetitive, due to the presence of families of genes that are present in multiple copies (>10). As a result, sequencing based on short reads only would not be able to reconstruct the genome in its entirety. In addition, good quality full genome sequencing of ASFV is complicated by persistence contamination with fragments of host DNA. We are developing a novel protocol that will allow the sequencing of the virus using long-read technologies (PacBio or Oxford Nanopore). Probe-capture libraries have been designed to mitigate the problem of host contamination by selectively purifying viral DNA from a wide range of different ASFV genotypes. Protocols are being developed to prepare high quality high molecular weight viral DNA from different sample types suitable for generating sequencing libraries for long-read sequencing. |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2019 |
| Provided To Others? | No |
| Impact | More than 100 samples from 18 different LMIC countries have been identified and collated for sequencing with the new protocol. They will be added to the web-portal for transboundary pathogens which is currently being developed thanks to BBSRC funding. |
| Title | Method to evenly load flowcells with multiple samples |
| Description | Pirbright has an Illumina NextSeq sequencing machine installed in containment. Given the large amount of sequencing reads produced by each run, multiple samples are usually multiplexed on the same flowcell. However, determining the correct concentrations needed to obtain the same amount of reads for each sample is a hard problem, and usually requires a number of expensive and material-consuming kits to be run prior to sequencing. We have developed a method that allows samples to be evenly multiplexed based only on Bioanalyzer data (we routinely run Bioanalyser on the samples anyway as a form of quality control). The method works well, and reliably across a wide range of sample concentrations and sequenced organisms. |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2018 |
| Provided To Others? | No |
| Impact | The method greatly increased the quality of our output, and drastically simplified our workflow by eliminating the external quantification steps that were previously required. |
| Title | Optimisation of chicken kidney cell culture preparation |
| Description | We have optimised the method of extracting kidneys and preparing chicken kidney (CK) cell cultures from chickens. CK cells are used for in vitro assays to study avian viruses, in particular infectious bronchitis virus. |
| Type Of Material | Cell line |
| Year Produced | 2017 |
| Provided To Others? | No |
| Impact | This has increased the yield of viable cells and the quality of the cell cultures. We can now obtain the number of cells we require using fewer chicken kidneys, therefore requiring fewer chickens to be culled. This is an important development for the 3Rs. We hope to publish this method for others' information. |
| Title | Panel of ACE2 proteins for screening SARS-CoV-2 host range |
| Description | A biological library of ACE2 expression constructs representing receptors from 22 different human and animal species. |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2020 |
| Provided To Others? | Yes |
| Impact | Establishment of surveillance tools to examine the potential reverse zoonotic spillover of SARS-CoV-2 into anima species |
| Title | Recombinant antibody generation pipeline - WM |
| Description | Validated a sequencing protocol at Pirbright for mouse hybridomas, cattle heterohybridomas, and porcine B cells. Vector constructed for humans, mouse, cattle, pigs and chicken to allow ligation of commercially generated antibody gene blocks or PCR products generated by degenerate or 5' RACE PCR. These methods allow the following: • Sequencing mAbs • Making Fab fragments from mAbs • Class switching mAbs • Species switching mAbs |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2018 |
| Provided To Others? | Yes |
| Impact | The methods developed have enabled a standardised pipeline that allows cloning of PCR products or synthetic gene constructs for recombinant antibody generation from various species. This uniform approach avoids development of bespoke protocols for each species thus saving time and resources. |
| URL | http://www.immunologicaltoolbox.co.uk |
| Title | Stable chicken cell lines overexpressing chicken IFITM1,2 and 3 |
| Description | In collaboration with Mark Fife, we have produced chicken stable cell lines overexpressing chicken IFITM1,2, 3 and a mutant chicken IFITM3 lacking palmitoylation sites. |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2017 |
| Provided To Others? | No |
| Impact | These cell lines will be used to evaluate the effect of the IFITM proteins on the replication of different viruses, including IBDV. |
| Title | UniMMap- a pipeline for mapping RNAseq data over repetitive immune complexes. |
| Description | To exploit the abundance of available short read sequencing data we have developed a pipeline that uses mappability to accurately measure transcription over repetitive gene complexes. This methods uses known haplotypes to examine regions of uniqueness, and then the RNAseq data from the individual to train the method to be species or individual specific. This is particularly important over gene complexes that contain genes involved in the immune system, that are often highly similar in sequence but can have profoundly different functions. |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2018 |
| Provided To Others? | No |
| Impact | the immediate impact will be adding fine resolution data to livestock gene expression atlas projects lead by the FAANG consortium. Further impact will be arising as this method is published and we apply it to numerous existing datasets the are publically avaialable as well as generated at Pirbright and by our collaborators. |
| Title | Use of reverse genetics to develop PPR DIVA vaccines |
| Description | Reverse genetics technique has been established for PPR virus in our laboratory. As PPR vaccine is a live attenuated virus, it is not possible to differentiate between vaccinated and infected animals (DIVA) in existing antibody assays. However using reverse genetics technique we have manipulated/mutated residues in the full-length cDNA of virus and rescued the live attenuated vaccine strain which worked as a DIVA vaccine. Using this technique a GFP ( Green fluorescent protein) has been introduced into the virulent PPR virus that helped to follow the virus in the infected goats. Similarly using this technique we have modified the existing live attenuated viruses ( Nigeria 75/1) and Sungri 96/1) in to recombinant marker vaccines that enables to differentiate between infection and vaccination ( DIVA). So we have demonstrated that reverse genetics tool can be used to study the pathogenesis and to develop the marker vaccines. This technique can be adapted for other negative strand viruses to design the DIVA vaccines. |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2015 |
| Provided To Others? | Yes |
| Impact | PPR DIVA vaccine developed that can differentiate between vaccinated and infected animals. This will be helpful to know the efficacy of vaccine by knowing the presence antibodies in animal is due to vaccination or infection. This will reduce the eradication time and will facilitate to declare freedom of diseases as soon as Possible without long waiting period. |
| Title | chimeric IBDVs |
| Description | Andrew Broadbent has engineered chimeric IBDVs expressing individual genes from a very virulent strain in the backbone of an attenuated strain. |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2018 |
| Provided To Others? | No |
| Impact | These chimeric strains can be used to define viral determinants of virulence. |
| Title | replication competent recombinant split-GFP infectious bursal disease virus |
| Description | We have generated a replication competent recombinant split-GFP infectious bursal disease virus (IBDV) where a small GFP11 molecule is tagged to the virus polymerase (VP1) to make IBDV-VP1-GFP11. Infected cells that express the GFP1-10 molecule fluoresce green when the GFP11 tag complements the GFP1-10. The IBDV-VP1-GFP11 colocalises with VP3 and dsRNA and therefore is likely to be present at the sites of virus replication. |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2017 |
| Provided To Others? | No |
| Impact | This tool can be used to study IBDV replication complex development in infected cells. |
| Title | tetracysteine-tagged IBDV |
| Description | Andrew Broadbent has engineered a tetracysteine (TC)-tagged IBDV that can be used in live cell imaging and co-infection studies with the split-GFP IBDV. |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2018 |
| Provided To Others? | No |
| Impact | This tool, combined with the split-GFP IBDV, will enable us to study co-infection, super-infection exclusion, and the intracellular requirements for viral reassortment. |
| Title | Analysis of paramyxovirus replication using Incucyte real-time imager |
| Description | We have optimised a data analysis pipeline for the Incucyte real-time imager to allow us to track viral replication in a high-throughput fashion (up to 6 x 96-well plates). This is based on simultaneous analysis of phase, as well as green and red fluorescence. |
| Type Of Material | Data analysis technique |
| Year Produced | 2018 |
| Provided To Others? | No |
| Impact | Only preliminary data to date. |
| Title | Boosting the FM-index on the GPU |
| Description | The FM-index is a data structure that lies at the core of most modern short-read aligners. Our work describes what is perhaps the first implementation of the FM-index on the GPU able to take full advantage of the high hardware parallelism (large number of computing cores) offered by modern Graphical Processing Units. |
| Type Of Material | Computer model/algorithm |
| Year Produced | 2016 |
| Provided To Others? | No |
| Impact | Short-read aligners are instrumental to the analysis of high-throughput sequencing experiments. As alignment is central to most analysis pipelines, speeding it up is essential to improve the performance of many bioinformatics tools. Thanks to our new algorithm FM-index based searches can be sped up by a large factor on all computers where a suitable GPU is present. |
| Title | Data from: A field study evaluating the humoral immune response in Mongolian sheep vaccinated against sheeppox virus |
| Description | Sheeppox is a transboundary disease of sheep caused by infection with the capripoxvirus sheeppox virus (SPPV). Sheeppox is found in Africa, the Middle East and Asia and is characterised by fever, multifocal cutaneous raised lesions, and death, with substantial negative impact on affected flocks. Vaccination with live attenuated capripoxvirus (CPPV) strains is an effective and widely used means of controlling sheeppox outbreaks, however there are few reports of post-vaccination field surveillance studies of sheeppox. This study used a commercially available ELISA and a fluorescence-based neutralisation assay (FVNT) to examine quantitative and temporal features of the humoral response of sheep vaccinated with a live attenuated CPPV strain in Mongolia. 400 samples were tested using the ELISA, and a subset of 45 also tested with the FVNT. There was substantial agreement between the FVNT and ELISA tests. Antibodies to CPPV were detected between 40 and 262 days post vaccination. There was no significant difference between serological status (positive / negative) and sex or age, however an inverse correlation was found between the length of time since vaccination and serological status. Animals between 90 and 180 days post-vaccination were more likely to be positive than animals greater than 180 days post vaccination. This data provides temporal parameters to consider when planning sheeppox post-vaccination monitoring programmes. In summary, our results show a commercial CPPV ELISA kit is a robust and reliable assay for use in resource-restricted low and low-middle income countries for post CPPV vaccination surveillance on a regional or national level. |
| Type Of Material | Database/Collection of data |
| Year Produced | 2020 |
| Provided To Others? | Yes |
| URL | http://datadryad.org/stash/dataset/doi:10.5061/dryad.msbcc2fvx |
| Title | FDA drug screening of RSV and SARS-CoV-2 cell-cell fusion |
| Description | Using inducible cell-cell fusion assays for SARS-CoV-2 and RSV we have screened the FDA approved use drug library for inhibitors of these viral-induced processes |
| Type Of Material | Data analysis technique |
| Year Produced | 2022 |
| Provided To Others? | No |
| Impact | No impact as yet, data still being validated in live virus assays |
| Title | Full length cattle MHC genes |
| Description | Using our method for full length MHC gene amplification, the largest reference set ever produced from common haplotypes has been sequenced and deposited in the publicly available data IPD-MHC with associated research tools. |
| Type Of Material | Database/Collection of data |
| Year Produced | 2020 |
| Provided To Others? | No |
| Impact | None yet, but it will allow far greater resolution when analysing MHC polymorphism and evolution in cattle populations. |
| URL | https://www.ebi.ac.uk/ipd/mhc/group/BoLA/ |
| Title | Method to detect the expression of repetitive genes |
| Description | Reconstructing expression levels of genes having a high repeat content is difficult, due to the impossibility of assigning RNA-sequencing that have multiple mappings to their correct originating location. In collaboration with the Immunogenetics group, the Integrative Biology and Bioinformatics groups are developing a data analysis method to circumvent the problem.The method has already been shown to be effective on some KIR genes in cattle. An essential component of the immune system, their family is rich in repeated members and repetitive regions, making the technique essential if one wants to study them. |
| Type Of Material | Data analysis technique |
| Year Produced | 2017 |
| Provided To Others? | No |
| Impact | Being able to resolve the expression of repetitive genes, which are plentiful in most mammalian genomes, would be essential to the high-precision study of many important gene families, including most of the genes of the immune system. The technique has already proven itself effective on a real-life problem, and holds the potential to be extended to many more biological systems |
| Title | Supporting data for "De novo assembly of the cattle reference genome with single-molecule sequencing" |
| Description | Major advances in selection progress for cattle have been made following the introduction of genomic tools over the past 10-12 years. These tools depend upon the Bos taurus reference genome (UMD3.1.1), which was created using now-outdated technologies and suffers from a variety of deficiencies and inaccuracies. We present the new reference genome for cattle, ARS-UCD1.2, based on the same animal as the original to facilitate transfer and interpretation of results obtained from the earlier version, but applying a combination of modern technologies in a de novo assembly to increase continuity, accuracy, and completeness. The assembly includes 2.7 Gb, and is >250x more continuous than the original assembly, with contig N50 >25 Mb and L50 of 32. We also greatly expanded supporting RNA-based data for annotation that identifies 30,396 total genes (21,039 protein coding). The new reference assembly is accessible in annotated form for public use. |
| Type Of Material | Database/Collection of data |
| Year Produced | 2019 |
| Provided To Others? | Yes |
| URL | http://gigadb.org/dataset/100669 |
| Title | Technique for analysing interferon stimulated gene inhibition of RSV cell-cell fusion |
| Description | We have developed the techniques and performed the experiments to examine the effect of over-expressing individual interferon stimulated genes on RSV (bovine and human) replication and cell-cell fusion. This was achieved through integration of library technologies (for the ISGs) and high throughout imaging platforms. |
| Type Of Material | Data analysis technique |
| Year Produced | 2021 |
| Provided To Others? | No |
| Impact | No impact yet as data has still to be published. Manuscript in preparation. |
| Title | Viral assembly pipeline |
| Description | The Integrative Biology and Bioinformatics group has developed a data analysis pipeline to obtain sensitive and robust viral assemblies out of high-throughput sequencing data. In spite of the relatively short genomic length of most viruses, assembling of viral sequences can be challenging due to several reasons, such as: low amount of material in the sample, which might require amplification, introducing biases, and/or generates low-quality sequences; uneven coverage due to low-quality material or the genomic material of the virus being RNA; the viral nucleic acid being almost lost in the background of nucleic acid of the host. Our pipeline overcomes most of those problems, and is even able to detect different viral strains being present together in the same sample. It can also be used on metagenomic environmental samples. |
| Type Of Material | Data analysis technique |
| Year Produced | 2017 |
| Provided To Others? | No |
| Impact | The pipeline is being deployed as the tool of choice for the nascent sequencing facility at Pirbright. It has been used by several groups at Pirbright, and the FMDV World Reference Lab, in order to assemble very different kinds of viruses. |
| Title | Viral variant calling pipeline |
| Description | The Integrative Biology and Bioinformatics group has developed a data analysis pipeline to obtain sensitive and robust variant calling for viral sequences out of high-throughput sequencing data. In spite of the relatively short genomic length of most viruses, calling variant for viral sequences can be challenging due to several reasons, such as: low amount of material in the sample, which might require amplification, introducing biases, and/or generates low-quality sequences; very high genome coverage, which slows down most existing variant callers; the presence of a potentially very high number of different haplotypes for RNA viruses, which is an unusual scenario in variant calling. Our fast and sensitive Bayesian pipeline overcomes most of those problems. |
| Type Of Material | Data analysis technique |
| Year Produced | 2017 |
| Provided To Others? | No |
| Impact | The pipeline is being deployed as the tool of choice for the nascent sequencing facility at Pirbright. It has been used by several groups at Pirbright, and the FMDV World Reference Lab, in order to assemble very different kinds of viruses. |
| Description | ASFV Structure |
| Organisation | University of Oxford |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | We have prepared samples for analysis |
| Collaborator Contribution | Electron tomographic analysis of the samples we provided. |
| Impact | None as yet. |
| Start Year | 2019 |
| Description | Anihwa Call 1 |
| Organisation | Friedrich Loeffler Institute |
| Country | Germany |
| Sector | Academic/University |
| PI Contribution | 1. Conducted challenge experiments in goats to study pathogenicity. 2. Developed NGS technology to sequence PPR whole genome. |
| Collaborator Contribution | FLI has conducted transmission study between different species ( Goats, pig, camel). For the first time they showed that pigs are clinically infected by PPR virus. |
| Impact | Joint Publications |
| Start Year | 2013 |
| Description | Assembly of CHIKV in host cells/JEOL TEM training |
| Organisation | National Cheng Kung University |
| Country | Taiwan, Province of China |
| Sector | Academic/University |
| PI Contribution | Visit to Tainan in May 2017, provided training in cellular imaging of virally infected cells. Gave a seminar entitled Bioimaging at The Pirbright Institute. Hosted two visitors for two weeks in Bioimaging at Pirbright, provided training in EM sample preparation and data interpretation. |
| Collaborator Contribution | Professor Shang-Rung Wu hosted me for one week in Taiwan. During the visit to Pirbright, the Taiwanese researchers provided training on JEOL 2100F TEM to Pirbright researchers. Professor Wu provided prepared samples for imaging at a later date. |
| Impact | No outputs yet |
| Start Year | 2017 |
| Description | BBSRC - Oxford University iCASE Studentship (October 2019- September 2023). Production and assessment of antiviral prophylactic properties of natural biomolecules against avian influenza and Newcastle disease viruses affecting poultry production |
| Organisation | University of Oxford |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | This collaboration between The Pirbright Institute (UK), Oxford University (UK) and industrial partners in Italy. This project will conceived and developed bu MI. The PhD student (Holly Everest) in my group is investigating the antiviral prophylactic properties of natural biomolecules against avian influenza and Newcastle disease viruses affecting poultry production. |
| Collaborator Contribution | The partners in Italy will produce and purify natural antiviral molecules effective against avian influenza and other poultry viruses. |
| Impact | This project started in January 2020. |
| Start Year | 2020 |
| Description | Belsham |
| Organisation | Technical University of Denmark |
| Country | Denmark |
| Sector | Academic/University |
| PI Contribution | Joint PhD studentship and ongoing collaboration on capsid assembly |
| Collaborator Contribution | Joint PhD studentship and ongoing collaboration on capsid assembly |
| Impact | Joint PhD studentship Further funding |
| Start Year | 2016 |
| Description | Belsham |
| Organisation | University of Copenhagen |
| Country | Denmark |
| Sector | Academic/University |
| PI Contribution | Joint PhD studentship and ongoing collaboration on capsid assembly |
| Collaborator Contribution | Joint PhD studentship and ongoing collaboration on capsid assembly |
| Impact | Joint PhD studentship Further funding |
| Start Year | 2016 |
| Description | Characterisation of ASFV isolates |
| Organisation | Friedrich Loeffler Institute |
| Country | Germany |
| Sector | Academic/University |
| PI Contribution | Full genome sequencing of uncharacterised ASFV isolates |
| Collaborator Contribution | Biological characterisation of ASFV isolates |
| Impact | Non as yet. |
| Start Year | 2020 |
| Description | Chicken Microbiome - Robert La Ragione - University of Surrey |
| Organisation | University of Surrey |
| Department | School of Veterinary Medicine |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Providing sequencing data of gut microbiome from chickens infected with avian influenza viruses. |
| Collaborator Contribution | Sharing expertise in the analysis of microbiome data |
| Impact | Joint manuscripts and funding opportunities are being explored |
| Start Year | 2019 |
| Description | Collaboration on replication of mumps virus in cells |
| Organisation | University of Copenhagen |
| Country | Denmark |
| Sector | Academic/University |
| PI Contribution | Intellectual input so far, training research assistant to prepare samples, imaging samples in transmission electron microscopes |
| Collaborator Contribution | Preliminary confocal microscopy to optimize sample conditions. Preparation of samples for EM. |
| Impact | No outcomes yet |
| Start Year | 2018 |
| Description | Collaboration on the development of intranasal live-attenuated vaccine against SARS-CoV-2 |
| Organisation | Lancaster University |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Contributed reagents to tests the immunogenicity and protective efficacy of an intranasal live-attenuated vaccine against SARS-CoV-2 in preclinical animal models including mice and hamster. |
| Collaborator Contribution | The partners undertook most of the work to complete the study detailed in the publication entitled "Immunogenicity and protective efficacy of an intranasal live-attenuated vaccine against SARS-CoV-2 in preclinical animal models". https://www.biorxiv.org/content/10.1101/2021.01.08.425974v1 Abstract of publication. The global deployment of an effective and safe vaccine is currently a public health priority to curtail the coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Here, we evaluated a Newcastle disease virus (NDV)-based intranasal vectored-vaccine in mice and hamsters for its immunogenicity, safety and protective efficacy in challenge studies with SARS-CoV-2. The recombinant (r)NDV-S vaccine expressing spike (S) protein of SARS-CoV-2 administrated via intranasal route in mice induced high levels of SARS-CoV-2-specific neutralizing immunoglobulin A (IgA) and IgG2a antibodies and T cell-mediated immunity. Hamsters vaccinated with two doses of vaccine showed complete protection from clinical disease including lung infection, inflammation, and pathological lesions after SARS-CoV-2 challenge. Importantly, a single or double dose of intranasal rNDV-S vaccine completely blocked SARS-CoV-2 shedding in nasal turbinate and lungs within 4 days of vaccine administration in hamsters. Taken together, intranasal administration of rNDV-S has the potential to control infection at the site of inoculation, which should prevent both the clinical disease and transmission to halt the spread of the COVID-19 pandemic. |
| Impact | The partnership resulted in a joint publication. "Immunogenicity and protective efficacy of an intranasal live-attenuated vaccine against SARS-CoV-2 in preclinical animal models". https://www.biorxiv.org/content/10.1101/2021.01.08.425974v1 Abstract of publication. The global deployment of an effective and safe vaccine is currently a public health priority to curtail the coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Here, we evaluated a Newcastle disease virus (NDV)-based intranasal vectored-vaccine in mice and hamsters for its immunogenicity, safety and protective efficacy in challenge studies with SARS-CoV-2. The recombinant (r)NDV-S vaccine expressing spike (S) protein of SARS-CoV-2 administrated via intranasal route in mice induced high levels of SARS-CoV-2-specific neutralizing immunoglobulin A (IgA) and IgG2a antibodies and T cell-mediated immunity. Hamsters vaccinated with two doses of vaccine showed complete protection from clinical disease including lung infection, inflammation, and pathological lesions after the SARS-CoV-2 challenge. Importantly, a single or double dose of intranasal rNDV-S vaccine completely blocked SARS-CoV-2 shedding in nasal turbinate and lungs within 4 days of vaccine administration in hamsters. Taken together, intranasal administration of rNDV-S has the potential to control infection at the site of inoculation, which should prevent both the clinical disease and transmission to halt the spread of the COVID-19 pandemic. |
| Start Year | 2020 |
| Description | Collaboration with Bauchi State Veterinary Services, Nigeria |
| Organisation | Bauchi State University |
| Country | Nigeria |
| Sector | Academic/University |
| PI Contribution | We have provided expertise on sheeppox, goatpox and lumpy skin disease virus diagnosis and epidemiology |
| Collaborator Contribution | Our collaborators in Bauchi State have provided virus samples and epidemiological data |
| Impact | Preliminary data was used to support successful funding via a BBSRC Impact Accelerator Award, and a Pathways to Impact project in BBSRC project BB/R008833/1 "Development of rationally designed live-attenuated lumpy skin disease vaccines" |
| Start Year | 2016 |
| Description | Collaboration with Keith Chappell on virus neutralisation |
| Organisation | University of Queensland |
| Country | Australia |
| Sector | Academic/University |
| PI Contribution | Assessment of neutralisation to a range of viral immunogens by VNT using pseudotyping technology Assessment of neutralisation to a range of viral immunogens by microfusion inhibition test |
| Collaborator Contribution | Provision of sera from vaccination studies Provision of constructs for pseudotyping and cell-cell fusion |
| Impact | None yet, publications in preparation |
| Start Year | 2020 |
| Description | Collaboration with Prof. Jane McKeating on RSV and SARS-CoV-2 virology |
| Organisation | University of Oxford |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Assessment of SARS-CoV-2 cell-cell fusion under various conditions, and with chemical inhibitors Provision of RSV reagents |
| Collaborator Contribution | Provision of inhibitors and other reagents Experimentation with RSV |
| Impact | None yet, paper under review |
| Start Year | 2020 |
| Description | Collaboration with State Central Veterinary Laboratory, Mongolia |
| Organisation | State Central Veterinary Laboratory |
| Country | Mongolia |
| Sector | Public |
| PI Contribution | We provided tools, techniques and expertise on sheeppox and goatpox |
| Collaborator Contribution | Our colleagues provided data and field samples |
| Impact | A workshop was held in Pirbright in March 2017, and a return workshop in Ulaanbaatar in July 2017. Preliminary data supported successful grant applications for an Impact Accelerator Award, and a Pathways to Impact project in BB/R008833/1 "Development of rationally designed live-attenuated lumpy skin disease vaccines". |
| Start Year | 2016 |
| Description | Collaboration with University of Cambridge |
| Organisation | University of Cambridge |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | We have performed in vitro experiments with infectious bronchitis virus, contributed to data analysis, preparation of manuscripts and discussed further funding opportunities. |
| Collaborator Contribution | Our partners have analysed viral transcription and translation, and host gene expression after infection of host cells with infectious bronchitis virus, prepared manuscripts and discussed further funding opportunities. |
| Impact | Dinan, A., Keep, S., Bickerton, E., Britton, P., Firth, A. and Brierley, I. (2019) Comparative analysis of gene expression in virulent and attenuated strains of infectious bronchitis virus at sub-codon resolution. J. Virol. 93(18) e00714-19. |
| Start Year | 2012 |
| Description | Collaboration with University of Copenhagen CFIM on cell biology of virus-host interactions |
| Organisation | University of Copenhagen |
| Country | Denmark |
| Sector | Academic/University |
| PI Contribution | Intellectual input into virus projects which come in to the microscopy core facility at the University. Limited practical work and training given to researchers there. Includes lecturing/organisation of MSc and PhD courses at Copenhagen. PH awarded Visiting Professorship. |
| Collaborator Contribution | Research projects are presented to the core facility, I advise on the best route for achieving the data required and those researchers complete preliminary data following my advice. Collaborators are then able to produce the best possible samples for further advanced microscopy as necessary. |
| Impact | No tangible outcomes yet as we are in early stages. This is multi-disciplinary as projects can come from several University departments (medical, pathology, cell biology, veterinary, food), and other Universities in Denmark (DTU). University awarded Visiting Professorship |
| Start Year | 2018 |
| Description | Collaborative PhD student project on virus-bacteria-host-vector interaction |
| Organisation | University of Edinburgh |
| Department | The Roslin Institute |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | This is a collaborative PhD studentship funded by The Pirbright Institute between Pirbright and the Roslin Institute. The student is investigating how bacteria or bacterial compounds will effect bluetongue virus replication in mammalian host cells with a specific emphasis on skin derived migratory cells. While mainly based at Pirbright the student will visit the Roslin Institute and spend some time in the lab of Prof. Jane Hope. |
| Collaborator Contribution | The student will carry out the main laboratory work at Pirbright including infection studies of ruminant primary host target cells in the presence/ absence of bacterial compounds. There will be regular meetings between the students and supervisors at Pirbright and at Roslin (at least monthly by skype) and the student will visit the Roslin group for specific work aspects such as flow cytometry of skin derived pseudo-afferent lymph cells. Roslin will also make cattle pseudo-afferent lymph cells available to the student and Pirbright for ex vivo BTV infection studies. |
| Impact | bacteriology, virology, immunology, virus-host interaction |
| Start Year | 2019 |
| Description | Collaborative PhD studentship on the mechanisms and importance of BTV co-infections on viral reassortment and diversity |
| Organisation | University of Surrey |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Expertise on BTV infections of relevant host and vector cells, carrying out synchronous and asynchronous BTV co-infection between multiple strains, identification of superinfection exclusion, assay development for BTV strain specific detection, NGS sequencing |
| Collaborator Contribution | Expertise on analysis of large data sets especially bioinformatic analysis of NGS sequence data and identification of reassortment, application of advanced mathematical analysis |
| Impact | Shared supervision of a PhD candidate, regular team meetings, training and development of a PhD candidate across both organizations, inter-disciplinary exchange between virology, genetics, molecular diagnostics, NGS sequencing and bioinformatics, mathematical modelling |
| Start Year | 2021 |
| Description | Collaborative network with Ed Wright, Nigel Temperton and Giada Mattiuzzo on SARS-CoV-2 pseudotyping |
| Organisation | National Institute for Biological Standards and Control (NIBSC) |
| Country | United Kingdom |
| Sector | Public |
| PI Contribution | Development and establishment of SARS-CoV-2 research tools, including pseudotypes and cell-cell fusion assays |
| Collaborator Contribution | Provision of reagents, protocols and technical guidance |
| Impact | We aided in NIBSC's development of WHO international standard for SARS-CoV-2 and have a publication in press on sarbecovirus neutralisation: bioarchived here https://www.biorxiv.org/content/10.1101/2021.08.17.456606v1 |
| Start Year | 2020 |
| Description | Collaborative network with Ed Wright, Nigel Temperton and Giada Mattiuzzo on SARS-CoV-2 pseudotyping |
| Organisation | University of Kent |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Development and establishment of SARS-CoV-2 research tools, including pseudotypes and cell-cell fusion assays |
| Collaborator Contribution | Provision of reagents, protocols and technical guidance |
| Impact | We aided in NIBSC's development of WHO international standard for SARS-CoV-2 and have a publication in press on sarbecovirus neutralisation: bioarchived here https://www.biorxiv.org/content/10.1101/2021.08.17.456606v1 |
| Start Year | 2020 |
| Description | Collaborative network with Ed Wright, Nigel Temperton and Giada Mattiuzzo on SARS-CoV-2 pseudotyping |
| Organisation | University of Sussex |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Development and establishment of SARS-CoV-2 research tools, including pseudotypes and cell-cell fusion assays |
| Collaborator Contribution | Provision of reagents, protocols and technical guidance |
| Impact | We aided in NIBSC's development of WHO international standard for SARS-CoV-2 and have a publication in press on sarbecovirus neutralisation: bioarchived here https://www.biorxiv.org/content/10.1101/2021.08.17.456606v1 |
| Start Year | 2020 |
| Description | Collaborative project between The Pirbright Institute, UK and Miyazaki University, Japan |
| Organisation | University of Miyazaki |
| Country | Japan |
| Sector | Academic/University |
| PI Contribution | Provision of expertise in foot-and-mouth virus research; use of high containment facilities to perform experiments |
| Collaborator Contribution | Expertise in tetramer production, analysis and provided financially towards a travel bursary. |
| Impact | The protection afforded by most foot-and-mouth disease (FMD) vaccines depends to a large extent on humoral immunity, dictating the need to characterise the underlying mechanisms that mediate antibody production. To address this, we have developed novel major histocompatibility complex (pMHC) class II tetramers to identify antigen specific CD4+ T-cells that are present following vaccination against FMD. To date, we have used a MHC II tetramer (MHC serotype: A18) and peripheral blood mononuclear cells (PBMCs) from MHC-matched cattle vaccinated against FMD (O serotype). Importantly, we have identified CD4+ T-cells that recognise 15 epitopes (identified from 137 peptides) in the FMDV structural proteins; one in VP1, five in VP2, six in VP3 and three in VP4. This work will further our understanding of the repertoire of capsid epitopes recognised by the host's immune response following FMD vaccination and facilitate the identification of new correlates of protection. A first manuscript is in the final stages of preparation and will be submitted shortly. |
| Start Year | 2017 |
| Description | Collaborative studentship PPRV |
| Organisation | University of Surrey |
| Department | School of Veterinary Medicine |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Practical laboratory work and in vivo animal studies carried out |
| Collaborator Contribution | Intellectual input in scientific remit of studentship, training of student, use of facilities and lab |
| Impact | DOI: 10.1128/JVI.01471-18 Thesis: The immune response to live, attenuated peste des petits ruminants virus vaccines |
| Start Year | 2014 |
| Description | Commonwealth Scholarship. Molecular determinants impacting avian influenza H9N2 virus evolution, replication fitness and virulence |
| Organisation | Royal Veterinary College (RVC) |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | I develop a research project proposal in which we are investigating how evolutionary changes in the H9N2 avian influenza virus modulate virus fitness and virulence in chickens. This PhD studentship project was funded by Commonwealth scholarship commission for 3 years. |
| Collaborator Contribution | The collaborating Partner Dr Nicola Lewis at Royal Veterinary College is a co-supervisor of the PhD student Thusitha Karunarathna. she provide necessary guidance to achieve prescribed objectives of this project. |
| Impact | The project is at its initial stage. |
| Start Year | 2019 |
| Description | Components of the Medea gene drive system in a mosquito Aedes aegypti |
| Organisation | National Health Research Institutes (NHRI) Taiwan |
| Department | Institute of Molecular and Genomic Medicine |
| Country | Taiwan, Province of China |
| Sector | Charity/Non Profit |
| PI Contribution | Developed joined research project and lead successful application for funding |
| Collaborator Contribution | Various lines of research, including construction of plasmids and development and characterisation of transgenic mosquito strains; and group discussions about results, conclusions and research progress. |
| Impact | No impact yet. |
| Start Year | 2018 |
| Description | Components of the Medea gene drive system in a mosquito Aedes aegypti |
| Organisation | National Institute of Genetics |
| Department | NIG-FLY, Mishima (Drosophila RNAi resources) |
| Country | Japan |
| Sector | Academic/University |
| PI Contribution | Developed joined research project and lead successful application for funding |
| Collaborator Contribution | Various lines of research, including construction of plasmids and development and characterisation of transgenic mosquito strains; and group discussions about results, conclusions and research progress. |
| Impact | No impact yet. |
| Start Year | 2018 |
| Description | Dengue microvascular pathogenesis |
| Organisation | University of Surrey |
| Department | Faculty of Health and Medical Sciences |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | I brought my expertise in dengue and virology, and had significant intellectual input into shaping the research question and methodological approach. I also directly contributed to the training of one shared MSc student, one shared PhD student and three undergraduate students who have contributed to this project so far. |
| Collaborator Contribution | The partner (Dr. Paola Campagnolo, University of Surrey) brought their expertise in cardiovascular biology and had significant intellectual input into shaping the research question and methodological approach. They also directly contributed to the training of one shared MSc student, one shared PhD student and three undergraduate students who have contributed to this project so far. |
| Impact | The collaboration has resulted in the following publications: DOI: 10.1128/mSphere.00258-20 DOI: 10.1128/mSphere.00258-20 This is an interdisciplinary collaboration. The disciplines are microbiology/virology (me) and cardiovascular biology (Dr. Paola Campagnolo, University of Surrey). |
| Start Year | 2018 |
| Description | Development of a bivalent PRRS/Nipah virus vaccine |
| Organisation | National Center for Genetic Engineering and Biotechnology (BIOTEC) |
| Country | Thailand |
| Sector | Public |
| PI Contribution | Expertise in PRRSV and Nipah virus vaccine research |
| Collaborator Contribution | Expertise in genetically engineering PRRSV |
| Impact | Too early. |
| Start Year | 2020 |
| Description | Dr Carol Cardona, University of Minnesota- avian influenza virus challenge of IBDV or mock-infected chickens |
| Organisation | University of Minnesota |
| Country | United States |
| Sector | Academic/University |
| PI Contribution | We will challenge birds that have been exposed to IBDV, or mock exposed, with highly pathogenic avian influenza (HPAI) strains to determine how IBDV-mediated immunosuppression impacts upon HPAI infection in chickens. |
| Collaborator Contribution | Dr Cardona's group will challenge birds that have been exposed to IBDV, or mock exposed, with low pathogenic avian influenza (LPAI) strains to determine how IBDV-mediated immunosuppression impacts upon LPAI infection in chickens. |
| Impact | we have been successful in obtaining funding (Grant Ref: BB/T008806/1) |
| Start Year | 2019 |
| Description | Dr Holly Shelton, The Pirbright Institute- avian influenza virus challenge of IBDV or mock-infected chickens |
| Organisation | The Pirbright Institute |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | We will expose birds to IBDV, compared to mock controls |
| Collaborator Contribution | Holly will train my group in conducting studies with avian influenza viruses and, together, we will challenge birds with highly pathogenic avian influenza (HPAI) strains to determine how IBDV-mediated immunosuppression impacts upon HPAI infection in chickens. |
| Impact | we have been successful in obtaining funding (Grant Ref: BB/T008806/1) |
| Start Year | 2019 |
| Description | Dr Liam Morrison |
| Organisation | University of Edinburgh |
| Department | The Roslin Institute |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Analysis is single cattle B cell antibody sequences |
| Collaborator Contribution | Single cattle B cell antibody sequences |
| Impact | None as yet |
| Start Year | 2019 |
| Description | Dr Lyndsay Fry |
| Organisation | Washington State University |
| Department | Washington State University Spokane |
| Country | United States |
| Sector | Academic/University |
| PI Contribution | Single B cell sorting and antibody sequencing, developing a new methods for Theileria sp. |
| Collaborator Contribution | Providing funding and the samples from immunised animals. We have also engineered an antibody to aid in the sorting of cattle B cells |
| Impact | None as yet |
| Start Year | 2019 |
| Description | Dr Michael Skinner, Imperial College London |
| Organisation | Imperial College London |
| Department | Department of Primary Care and Public Health |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | We aim to compare the host transcriptional response of primary chicken B cells infected with different strains of IBDV of varying virulence. We have the specialist reagents and expertise in house that are required to culture primary chicken B cells, and we have access to different strains of IBDV. |
| Collaborator Contribution | Dr Michael Skinner and his team at Imperial College London have the expertise and facilities to generate and analyse RNA-Seq data in order to determine host transcriptional responses. This is required in order to complete the aim of the project. |
| Impact | Further funding: PhD studentship (£102,800) - Dr Andrew Broadbent at The Pirbright Institute and Dr Michael Skinner at Imperial College London are joint supervisors for a PhD student working on this project. Further Funding: Houghton Trust (£8,000)- Dr Andrew Broadbent was awarded a Houghton Trust Small Research Grant to contribute to this project. Further funding: NC3Rs (Grant Ref: NC/R001138/1) |
| Start Year | 2014 |
| Description | Dundee |
| Organisation | University of Dundee |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Co-INvestigator on BBSRC sLoLa award |
| Collaborator Contribution | Co-INvestigator on BBSRC sLoLa award |
| Impact | BBSRC sLoLa award ongoing |
| Start Year | 2012 |
| Description | Edinburgh - Haas |
| Organisation | University of Edinburgh |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Co-Investigator on BBSRC sLoLa award Co-Investigator on BBSRC project grant Collaboration in virus-host interactions |
| Collaborator Contribution | Co-Investigator on BBSRC sLoLa award Co-Investigator on BBSRC project grant Collaboration in virus-host interactions |
| Impact | BBSRC sLoLa award BBSRC project grant |
| Start Year | 2012 |
| Description | Establishment of a novel poultry vaccine platform inducing rapid and strong immunity through targeted delivery of antigens to chicken immune cells |
| Organisation | MSD Animal Health |
| Country | United Kingdom |
| Sector | Private |
| PI Contribution | The research focus is to develop next-generation of poultry vaccines that induce rapid and strong immune responses in chickens. The candidate vaccine constricts will be developed at the Pirbright Institute |
| Collaborator Contribution | The collaborating partner "MSD Animal Health" will evaluate the potency and efficacy for registration and feasibility for commercial production. |
| Impact | Based on the project preliminary data a new project was developed entitled Protecting poultry from avian influenza, Newcastle disease, infectious bronchitis, and Gumboro disease with a single dose of a multivalent vaccine". This project further strengthened the collaborative research work with MSD Animal Health. |
| Start Year | 2021 |
| Description | Evaluation of COVID-19 vaccine candidate immunogenicity in pigs |
| Organisation | Imperial College London |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Expertise in evaluating vaccine immunogenicity in pigs |
| Collaborator Contribution | Provided COVID-19 vaccine candidates |
| Impact | Joint publication on the evaluation of ChAdOx1 nCoV-19 (AZD1222) (https://doi.org/10.1038/s41541-020-00221-3). Data included in in the pre-clinical dossier submitted to regulators (e.g., European Medicines Agency) and supported the successful Marketing Authorisation application. |
| Start Year | 2020 |
| Description | Evaluation of COVID-19 vaccine candidate immunogenicity in pigs |
| Organisation | University of Oxford |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Expertise in evaluating vaccine immunogenicity in pigs |
| Collaborator Contribution | Provided COVID-19 vaccine candidates |
| Impact | Joint publication on the evaluation of ChAdOx1 nCoV-19 (AZD1222) (https://doi.org/10.1038/s41541-020-00221-3). Data included in in the pre-clinical dossier submitted to regulators (e.g., European Medicines Agency) and supported the successful Marketing Authorisation application. |
| Start Year | 2020 |
| Description | Evaluation of a PRRSV-vectored Nipah virus vaccine |
| Organisation | National Center for Genetic Engineering and Biotechnology (BIOTEC) |
| Country | Thailand |
| Sector | Public |
| PI Contribution | Expertise in porcine immunology/vaccinology, inc. PRRSV and Nipah |
| Collaborator Contribution | Expertise in PRRSV reverse genetics |
| Impact | None as yet |
| Start Year | 2019 |
| Description | Evaluation of bovine herpesvirus-4 as a vaccine vector for pigs |
| Organisation | University of Parma |
| Country | Italy |
| Sector | Academic/University |
| PI Contribution | Expertise in porcine vaccinology/immunology |
| Collaborator Contribution | Expertise in the generation of recombinant bovine herpesvirus-4 (BoHV-4) vectors expressing heterologous antigens |
| Impact | We demonstrated that immunisation of pigs with recombinant BoHV-4 vectors expressing Nipah virus glycoproteins are highly immunogenic in pigs. Most promising and as hypothesised, were the potent CD4 and CD8 T cell responses which were an order of greater than those induced by other viral vaccine vectors. These data have led to the external funding of projects by two companies to evaluate BoHV-4 vectors in the context of other porcine diseases. |
| Start Year | 2018 |
| Description | Evaluation of immune checkpoint inhibitors as molecular adjuvants for improved PRRSV vaccines |
| Organisation | Leidos |
| Country | United States |
| Sector | Private |
| PI Contribution | Expertise in PRRSV immunology |
| Collaborator Contribution | Patented immune checkpoint inhibitors |
| Impact | No yet |
| Start Year | 2019 |
| Description | Evaluation of mRNA vectored vaccines in pigs |
| Organisation | University of Pennsylvania |
| Country | United States |
| Sector | Academic/University |
| PI Contribution | Expertise in porcine immunology/vaccinology |
| Collaborator Contribution | Expertise in mRNA vaccines |
| Impact | We evaluated in pigs the immunogenicity an mRNA vector encoding a Nipah virus glycoprotein. This vaccine formulation induced high virus neutralising antibody titres (comparable with protein subunit in adjuvant) and T cell responses (comparable with viral vectors). We also utilised mRNA immunised pigs to isolate Nipah virus neutralising monoclonal antibodies. |
| Start Year | 2018 |
| Description | Evaluation of multivalent viral hemorrhagic fever vaccine candidates immunogenicity in pigs |
| Organisation | Imperial College London |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Supported the pre-clinical development of multivalent viral hemorrhagic fever vaccine candidates by evaluating their immunogenicity in pigs |
| Collaborator Contribution | Conceived and produced vaccine candidates |
| Impact | Still ongoing. |
| Start Year | 2021 |
| Description | Evaluation of solid dose vaccines for livestock |
| Organisation | Enesi Pharma |
| Country | United Kingdom |
| Sector | Private |
| PI Contribution | Expertise in PRRSV and vaccine evaluation |
| Collaborator Contribution | Expertise in vaccine formulation |
| Impact | Multidisciplinary collaboration between formulation scientist and viral immunologists. |
| Start Year | 2019 |
| Description | Exploiting mosquito immunity for generating refractory mosquitoes |
| Organisation | The Pirbright Institute |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | I brought my expertise in mosquito immunology and viral evasion of innate immune responses in vertebrates and invertebrates. I provided intellectual input into the project and provided training for one research assistant. |
| Collaborator Contribution | The partner (Luke Alphey, The Pirbright Institute) brought their expertise in mosquito transgenesis and molecular approaches to developing refractory mosquitoes that cannot transmit viruses. The partner also provided intellectual input into the project. |
| Impact | The following publications are associated with this collaboration: DOI: 10.3390/v13112116 |
| Start Year | 2020 |
| Description | Genetic analysis orbiviruses |
| Organisation | University of Surrey |
| Department | Faculty of Health and Medical Sciences |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Virus sequences provided for analysis. Meeting and discussion for analysis by virologist employed on this project and biomathematican at the University of Surrey |
| Collaborator Contribution | Analysis of data, training of the virologist and computational and mathematical methodology, discussion about analysis strategies |
| Impact | The collaboration is multi-disciplinary - between mathematical modelling and virology. Data analysis is still ongoing but is envisaged as preliminary data for future funding applications |
| Start Year | 2017 |
| Description | HMS |
| Organisation | Harvard University |
| Department | Harvard Medical School |
| Country | United States |
| Sector | Academic/University |
| PI Contribution | Collaboration |
| Collaborator Contribution | Collaboration |
| Impact | Collaborative research |
| Description | High containment microscopy with Thermo Fisher |
| Organisation | Thermo Fisher Scientific |
| Country | United States |
| Sector | Private |
| PI Contribution | Provide advice on decontamination protocols |
| Collaborator Contribution | Development/optimisation of microscope decontamination procedures |
| Impact | No outputs yet, very early stages of collaboration |
| Start Year | 2019 |
| Description | Identification of conserved B-cell epitopes of highly pathogenic coronaviruses for broadspectrum immunotherapy and vaccine design. |
| Organisation | Imperial College London |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | We have performed experiments to generate preliminary data for a collaborative grant application, contributed to the writing of the grant proposal and responded to reviewers comments. |
| Collaborator Contribution | My collaborators have performed experiments to generate preliminary data for a collaborative grant application, contributed to the writing of the grant proposal and responded to reviewers comments. |
| Impact | Submission of proposal entitled "Identification of conserved B-cell epitopes of highly pathogenic coronaviruses for broadspectrum immunotherapy and vaccine design" to the "One Health Approaches to Accelerate Vaccine Development" call that forms part of the UK government's commitment to Official Development Assistance (ODA) in October 2017. |
| Start Year | 2017 |
| Description | Identification of conserved B-cell epitopes of highly pathogenic coronaviruses for broadspectrum immunotherapy and vaccine design. |
| Organisation | University of Kent |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | We have performed experiments to generate preliminary data for a collaborative grant application, contributed to the writing of the grant proposal and responded to reviewers comments. |
| Collaborator Contribution | My collaborators have performed experiments to generate preliminary data for a collaborative grant application, contributed to the writing of the grant proposal and responded to reviewers comments. |
| Impact | Submission of proposal entitled "Identification of conserved B-cell epitopes of highly pathogenic coronaviruses for broadspectrum immunotherapy and vaccine design" to the "One Health Approaches to Accelerate Vaccine Development" call that forms part of the UK government's commitment to Official Development Assistance (ODA) in October 2017. |
| Start Year | 2017 |
| Description | Identification of sows with antibodies broadly neutralising PRRSV |
| Organisation | University of Lleida |
| Country | Spain |
| Sector | Academic/University |
| PI Contribution | Expertise in PRRSV immunology |
| Collaborator Contribution | Access to large numbers of sows routinely vaccinated and exposed to PRRSV |
| Impact | Not yet |
| Start Year | 2019 |
| Description | Imperial |
| Organisation | Imperial College London |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Joint supervision of PhD student |
| Collaborator Contribution | Joint supervision of PhD student |
| Impact | One successful completed PhD student. Papers published or in preparation. Another PhD student in progress. |
| Start Year | 2010 |
| Description | Industrial partnership-Aviagen 2018 |
| Organisation | Aviagen Group |
| Country | United States |
| Sector | Private |
| PI Contribution | We have established a significant industrial partnership with Aviagen group Ltd. The nature of this work remains confidential. |
| Collaborator Contribution | The nature of this work remains confidential. |
| Impact | The nature of this work remains confidential. |
| Start Year | 2018 |
| Description | Interferon stimulated genes |
| Organisation | Royal Veterinary College (RVC) |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Hosted meetings and carried out preliminary experiments |
| Collaborator Contribution | Contributed reagents and expertise |
| Impact | Preliminary data was used to support an MSc project. This has since led to a publication and a PhD project. |
| Start Year | 2016 |
| Description | Investigating antigenic determinants inducing stronger and broader cross-protective immunity among H5 avian influenza viruses |
| Organisation | Royal Veterinary College (RVC) |
| Department | Veterinary Basic Sciences |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | The overarching aims of the proposed research are to improve controls against H5 subtype of avian influenza viruses infecting poultry by investigating underlying mechanisms that define how vaccines renders effectiveness and develop novel approaches enhancing the effectiveness of H5 avian influenza vaccines. We recently generated a panel of monoclonal antibodies (mAbs) that recognise a variety of different clades of H5 viruses. Propagation of AIV in eggs or cultured cells together with mAbs drives virus to escape from mAbs neutralisation activity. These classical virus neutralization assays (VN) followed by HA gene sequence analysis allowed us to defined the role of amino acid substitutions that are associated with evasion of antibody neutralization through emergence of antigenic variants and failure of vaccine efficacy. |
| Collaborator Contribution | provided intellectual input for achieving prescribed objectives of this work. |
| Impact | Talk entitled "Molecular determinants for antigenicity and vaccine efficacy of avian influenza viruses" was presented at Newton Agham Researcher Links Workshop " Novel Vaccines and Diagnostic Technologies against Emerging and re-emerging Veterinary Pathogens" at Rizal Park Hotel, Manila, Philippines, 4-7 February 2019. |
| Start Year | 2019 |
| Description | Investigating antigenic determinants inducing stronger and broader cross-protective immunity among H5 avian influenza viruses. |
| Organisation | Royal Veterinary College (RVC) |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | We developed research programme to characterise diversity of antigenic epitopes induce protective immunity in chickens against H5 subtypes of avian influenza viruses. |
| Collaborator Contribution | Collaborating partner Dr Nicola Lewis at Royal Veterinary College provide advise to on cartography programme to visualize the antigenic similarity or difference among different strains of influenza viruses. |
| Impact | The work under this collaborative determined the antigenic differences among different clades of H5 avian influenza viruses circulating in birds. This project is multi-disciplinary. our laboratory mainly work on virology and immunology part of the project and we seek help on computer modeling part from the collaborating partner Dr Nicola Lewis. |
| Start Year | 2019 |
| Description | Invitation to participate in the Vaccine Innovation Forum 2017 at Shanghai 26-27 April 2017 |
| Organisation | Chinese Academy of Agricultural Sciences |
| Department | Shanghai Veterinary Research Institute |
| Country | China |
| Sector | Public |
| PI Contribution | Prof. Nair was invited to participate in the Vaccine Innovation Forum 2017 at Shanghai 26-27 April 2017 to present a talk on 'Novel Vaccine Development for Avian Virus Diseases'. He also participated in the round table discussions on the challenges and opportunities in the vaccines and vaccination sector |
| Collaborator Contribution | Vaccine Innovation Forum will help in expanding the future collaborations and activities of the UK-China Centre of Excellence for Research on Avian Diseases (http://www.uk-china-cerad.org/). |
| Impact | organised joint meeting in China and the UK to discuss on challenges and opportunities |
| Start Year | 2017 |
| Description | JN - Preparation of capped mesoporpus silica nanoparticles |
| Organisation | Polytechnic University of Valencia |
| Country | Spain |
| Sector | Academic/University |
| PI Contribution | We were involved with testing the nanoparticles once they had been made. We also contributed purified antibody and shipped it to our collaborators for the generation of one type of nanoparticle, which we subsequently tested. We were involved with the intellectual design of experiments |
| Collaborator Contribution | Our partners generated the nanoparticles and shipped them to us for testing. Our partners were also involved with the intellectual design of experiments. |
| Impact | Experimental work performed to investigate the use of mesoporous silica nanoparticles to detect FMDV proteases in clinical samples |
| Start Year | 2016 |
| Description | JS - Production of monoclonal antibodies that recognise FMDV VP4 from mouse spleens |
| Organisation | University of Edinburgh |
| Department | The Roslin Institute |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | VLPs displaying the N-terminal 15 amino acids of FMDV were designed and produced at The Pirbright Institute and then mice were immunised with them. The spleens of the immunised mice were harvested and splenocytes stored. The response to the VP4 sequence was checked by ELISA and the mouse with the best response was selected to send the splenocytes for monoclonal production. |
| Collaborator Contribution | The collaborators took the splenocytes obtained from the mouse experiments and carried out the fusions. They then screened the supernatants for positive wells that detected the VP4 sequence. |
| Impact | Positive wells have been identified indicating some monoclonals that are specific for VP4 have been generated. These will be further screened for ability to recognise virus and to neutralise infection. |
| Start Year | 2017 |
| Description | LMB |
| Organisation | Medical Research Council (MRC) |
| Department | MRC Laboratory of Molecular Biology (LMB) |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Collaboration |
| Collaborator Contribution | Collaboration |
| Impact | Collaborative research |
| Start Year | 2016 |
| Description | Leeds FBS |
| Organisation | University of Leeds |
| Department | Leeds Institute of Molecular Medicine |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Research |
| Collaborator Contribution | Research and student supervision |
| Impact | Research |
| Start Year | 2009 |
| Description | Linking sex determination and dosage compensation in a mosquito Anopheles gambiae |
| Organisation | University of Zurich |
| Department | Institute of Molecular Life Sciences |
| Country | Switzerland |
| Sector | Academic/University |
| PI Contribution | Developed a new research program. |
| Collaborator Contribution | Provided intellectual input, contributed to drafting a joined grant proposal. |
| Impact | No output yet. |
| Start Year | 2019 |
| Description | Merial |
| Organisation | Sanofi |
| Department | Merial Plc |
| Country | Global |
| Sector | Private |
| PI Contribution | BBSRC Link award |
| Collaborator Contribution | BBSRC Link award |
| Impact | BBSRC Link award in progress |
| Start Year | 2016 |
| Description | Next-generation PRRSV vaccines |
| Organisation | Eco Animal Health Ltd |
| Country | United Kingdom |
| Sector | Private |
| PI Contribution | Know how in terms of genetically manipulating PRRSV and evaluating PRRSV vaccines. |
| Collaborator Contribution | Financial contribution and know how in terms of developing a commercial vaccine. |
| Impact | Too early. |
| Start Year | 2020 |
| Description | Next-generation PRRSV vaccines |
| Organisation | Huvepharma |
| Country | Bulgaria |
| Sector | Private |
| PI Contribution | Expertise in PRRSV virology and immunology |
| Collaborator Contribution | Funding |
| Impact | Too early, project ongoing. |
| Start Year | 2019 |
| Description | Nicolas Locker - Investigation of translation regulation by infectious bronchitis virus |
| Organisation | University of Surrey |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | My team provide experience in molecular virology and cellular interactions of coronaviruses including previous experience of accessory proteins regulating cellular translation. One PhD student has successfully completed his studies working jointly between our labs and a second is due to begin in October 2022. |
| Collaborator Contribution | Dr Locker's team provide expertise in viral regulation of cellular translation and stress granule signalling. Dr Locker is also providing protocols, advice and training for techniques including ribopuromycylation and ribosome profiling. |
| Impact | PhD student funding (studentship Oct 2017- Sept 2021) - Control of host cell translation by infectious bronchitis virus PhD student funding (studentship Oct 2022- Mar 2026) - Coronavirus regulation of cellular antiviral responses and cross species transmission |
| Start Year | 2016 |
| Description | Novel PRRS and swine influenza vaccines |
| Organisation | National Center for Genetic Engineering and Biotechnology (BIOTEC) |
| Country | Thailand |
| Sector | Public |
| PI Contribution | Expertise in PRRS immunology and vaccine evaluation. |
| Collaborator Contribution | Novel vaccine strains and SE Asian field strains |
| Impact | Collaborative grant proposal funded under the BBSRC Newton Fund UK-China-Philippines-Thailand Swine and Poultry Research Initiative |
| Start Year | 2017 |
| Description | Oxford - Strubi |
| Organisation | University of Oxford |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Collaboration on virus structural biology and joint PhD studentship |
| Collaborator Contribution | Collaboration on virus structural biology and joint PhD studentship |
| Impact | Collaboration on virus structural biology has produced a number of novel virus structures and new understanding of virus entry and packaging |
| Start Year | 2008 |
| Description | PRRS and Influenza co-nfection studies |
| Organisation | Government of Thailand |
| Department | National Science and Technology Development Agency (NSTDA) |
| Country | Thailand |
| Sector | Public |
| PI Contribution | The Pirbright team of 4 scientists with expertise in immunology and animal care visited Thailand in July 2018 to train Thai colleagues to perform animal experiments (Chiang Mai) and analyse immune responses by ELISPOT and Flow cytometry (Bangkok). This provided Thailand with trained personnel and expertise in testing vaccines in pigs. As a result of the joint work, the Chiang Mai animal facility is currently being renovated. Development of such an animal facility will promote the production and testing of more vaccines by Thai scientists. |
| Collaborator Contribution | Our Thai colleagues provided us with local Thai PRRS and Influenza virus strains which we use in our co-infection studies. |
| Impact | None yet |
| Start Year | 2018 |
| Description | Partnering with National Taiwan University |
| Organisation | National Taiwan University |
| Country | Taiwan, Province of China |
| Sector | Academic/University |
| PI Contribution | We have prepared and submitted an application for a BBSRC-Taiwan Partnering Award to study "Assembly of Recombinant Infectious Bronchitis Virus and Determination of Antigenic Sites that Confer Hemagglutination Activity". |
| Collaborator Contribution | The partners conceived the project and got in contact about submitting an application for funding together. |
| Impact | An application has been made for a BBSRC-Taiwan Partnering Award in November 2018. |
| Start Year | 2018 |
| Description | Prof. Subrayal Reddy |
| Organisation | Lancaster University |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Electron microscopy of nano particles |
| Collaborator Contribution | Provided the nano particles |
| Impact | Papers have been published |
| Start Year | 2019 |
| Description | Professor Helen Sang,The Roslin Institute - GFP1-10 expressing transgenic chickens |
| Organisation | University of Edinburgh |
| Department | The Roslin Institute |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | We have developed IBDV tagged to GFP11 |
| Collaborator Contribution | Helen Sang's group at the Roslin Institute have developed transcgenic chicken lines that constitutively express GFP1-10 in every cell. |
| Impact | we have been successful in obtaining funding (Grant Ref: BB/S014594/1) |
| Start Year | 2019 |
| Description | Protein Production UK (Formerly the Oxford Protein Production Facility) |
| Organisation | Rosalind Franklin Institute |
| Country | United Kingdom |
| Sector | Charity/Non Profit |
| PI Contribution | We are providing the sequences of antibodies from various species to add into the vectors at Oxford to produce species specific antibodies. We are also providing and devloping assays to measure recombinant antibody confirmation and binding. |
| Collaborator Contribution | PPUK is providing access to all their facilities as well as expertise. They are also providing their vector backbone as the template for antibody production. |
| Impact | None as yet |
| Start Year | 2018 |
| Description | RMS Training Focussed Interest Group |
| Organisation | The Royal Microscopical Society |
| Country | United Kingdom |
| Sector | Charity/Non Profit |
| PI Contribution | Expertise towards shaping the standards of UK microscopy training. |
| Collaborator Contribution | Reviewing and maintaining microscopy training across the UK. |
| Impact | Maintaining and improving training standards across the UK for microscopy. |
| Start Year | 2015 |
| Description | Rapid acquisition of mammalian characteristics by avian influenza virus in single host infections. |
| Organisation | Imperial College London |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Provided reagents and day to day technical help for undertaking this project and supervising postdoctoral researcher working on this project. Reagents include influenza reverse genetic plasmids and H9N2 and H7N9-specific antibodies. |
| Collaborator Contribution | this project will aim to understand AIV genetics that facilitate the rapid acquisition of mammalian adaptation characteristics in a single host infection. The partners with our collaborations determined adaptive genetic changes in the H9N2 avian influenza virus following infection in mice (mammalian hosts). Different strains of avian influenza Viruses generated by reverse gentic techniques were inoculated into mice and lungs sampled daily. RNA recovered from lung homogenates were deep sequenced and mutations arising were characterised for adaptation. Relative viral fitness and the rapidity of the accumulation of mutations was measured and compared amongst the viral strains. |
| Impact | The results of the our experimental studies showed the avian-origin viruses rapidly acquired mutations that increase virus fitness in mammalian species. Therefore, these viruses pose zoonotic and pandemic threat to public health. |
| Start Year | 2018 |
| Description | Rapid acquisition of mammalian characteristics by avian influenza virus in single host infections. |
| Organisation | The Pirbright Institute |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Provided reagents and day to day technical help for undertaking this project and supervising postdoctoral researcher working on this project. Reagents include influenza reverse genetic plasmids and H9N2 and H7N9-specific antibodies. |
| Collaborator Contribution | this project will aim to understand AIV genetics that facilitate the rapid acquisition of mammalian adaptation characteristics in a single host infection. The partners with our collaborations determined adaptive genetic changes in the H9N2 avian influenza virus following infection in mice (mammalian hosts). Different strains of avian influenza Viruses generated by reverse gentic techniques were inoculated into mice and lungs sampled daily. RNA recovered from lung homogenates were deep sequenced and mutations arising were characterised for adaptation. Relative viral fitness and the rapidity of the accumulation of mutations was measured and compared amongst the viral strains. |
| Impact | The results of the our experimental studies showed the avian-origin viruses rapidly acquired mutations that increase virus fitness in mammalian species. Therefore, these viruses pose zoonotic and pandemic threat to public health. |
| Start Year | 2018 |
| Description | Reannotation of the Anopheles gambiae genome |
| Organisation | Biomathematics and Statistics Scotland (BioSS) |
| Country | United Kingdom |
| Sector | Public |
| PI Contribution | Generated and provided RNA-seq data from the developmental transcriptome of the mosquito Anopheles gambiae Manually browsed the transcriptomic data in the genome context and provided coordinates for genome regions with not annotated genes or genes requiring annotation corrections. Generated and provided FAIRE-seq data from embryo samples of Anopheles gambiae to identify regulatory elements controlling early embryo development. |
| Collaborator Contribution | Conducting reannotation of the Anopheles gambiae genome based on RNA-seq data from the developmental transcriptome. Mapping the FAIRE-seq data to the genome and linking the mapped data the RNA-seq data. |
| Impact | No outputs yet |
| Start Year | 2017 |
| Description | Reannotation of the Anopheles gambiae genome |
| Organisation | Centre for Genomic Regulation (CRG) |
| Country | Spain |
| Sector | Academic/University |
| PI Contribution | Generated and provided RNA-seq data from the developmental transcriptome of the mosquito Anopheles gambiae Manually browsed the transcriptomic data in the genome context and provided coordinates for genome regions with not annotated genes or genes requiring annotation corrections. Generated and provided FAIRE-seq data from embryo samples of Anopheles gambiae to identify regulatory elements controlling early embryo development. |
| Collaborator Contribution | Conducting reannotation of the Anopheles gambiae genome based on RNA-seq data from the developmental transcriptome. Mapping the FAIRE-seq data to the genome and linking the mapped data the RNA-seq data. |
| Impact | No outputs yet |
| Start Year | 2017 |
| Description | Reannotation of the Anopheles gambiae genome |
| Organisation | Imperial College London |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Generated and provided RNA-seq data from the developmental transcriptome of the mosquito Anopheles gambiae Manually browsed the transcriptomic data in the genome context and provided coordinates for genome regions with not annotated genes or genes requiring annotation corrections. Generated and provided FAIRE-seq data from embryo samples of Anopheles gambiae to identify regulatory elements controlling early embryo development. |
| Collaborator Contribution | Conducting reannotation of the Anopheles gambiae genome based on RNA-seq data from the developmental transcriptome. Mapping the FAIRE-seq data to the genome and linking the mapped data the RNA-seq data. |
| Impact | No outputs yet |
| Start Year | 2017 |
| Description | Reporter PRRS viruses |
| Organisation | Kansas State University |
| Country | United States |
| Sector | Academic/University |
| PI Contribution | A new application for recombinant reporter PRRS viruses i.e. to use them as a high throughput screen for neutralizing (monoclonal) antibodies. Use as tools to assess the tropism of PRRSV for dendritic cell subsets and the functional consequences. |
| Collaborator Contribution | Provision of plasmids encoding GFP-expressing PRRSV-1 and -2. |
| Impact | Not yet |
| Start Year | 2016 |
| Description | Retroviral-mediated genetic programming of porcine memory B cells |
| Organisation | AIMM Therapeutics |
| Country | Netherlands |
| Sector | Private |
| PI Contribution | Provided a novel 'model' system to test their technology to isolate novel monoclonal antibodies |
| Collaborator Contribution | To provide their patented platform technology to genetically program porcine memory B cells to enable the isolation of memory B cells |
| Impact | Not yet |
| Start Year | 2016 |
| Description | SiSaf Ltd, FMDV + carriers |
| Organisation | SiSaf Ltd |
| Country | United Kingdom |
| Sector | Private |
| PI Contribution | Advice on experimental design, provision of FMDV empty capsids (Alison Burman). Will do practical work in the future. |
| Collaborator Contribution | Preparation of carrier formulations |
| Impact | No outputs. Company did not respond to proposed experimental plan. |
| Start Year | 2019 |
| Description | St Andrews |
| Organisation | University of St Andrews |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Co-investigator on BBSRC sLoLa |
| Collaborator Contribution | Lead-investigator on BBSRC sLoLa |
| Impact | BBSRC sLoLa ongoing |
| Start Year | 2012 |
| Description | Supply of Arthropods to External Partners |
| Organisation | APS Biocontrol |
| Country | United Kingdom |
| Sector | Private |
| PI Contribution | A total of approximately 300000 biting midges and several tens of thousand mosquitoes were supplied as a national capability to receiving institutes. These were used in a wide range of studies from assessment of host location to teaching resources. The grant funds a Culicoides technician (100% FTE) and a mosquito technician (50% FTE) who are responsible for production. All use is acknowledged in papers arising and research is conducted under MTAs throughout held by the Pirbright IP department. |
| Collaborator Contribution | A range of scientific studies around biting midges and mosquitoes. For outputs see publications. |
| Impact | Both internal and external outputs have resulted in a clearer understanding of the risk of arbovirus incursion in the UK and additionally has contributed to the development of control techniques for vectors. Outputs also include outreach activities, student training and other societal and cultural collaborations. |
| Start Year | 2011 |
| Description | Supply of Arthropods to External Partners |
| Organisation | Animal and Plant Health Agency |
| Country | United Kingdom |
| Sector | Public |
| PI Contribution | A total of approximately 300000 biting midges and several tens of thousand mosquitoes were supplied as a national capability to receiving institutes. These were used in a wide range of studies from assessment of host location to teaching resources. The grant funds a Culicoides technician (100% FTE) and a mosquito technician (50% FTE) who are responsible for production. All use is acknowledged in papers arising and research is conducted under MTAs throughout held by the Pirbright IP department. |
| Collaborator Contribution | A range of scientific studies around biting midges and mosquitoes. For outputs see publications. |
| Impact | Both internal and external outputs have resulted in a clearer understanding of the risk of arbovirus incursion in the UK and additionally has contributed to the development of control techniques for vectors. Outputs also include outreach activities, student training and other societal and cultural collaborations. |
| Start Year | 2011 |
| Description | Supply of Arthropods to External Partners |
| Organisation | Animal and Plant Health Agency |
| Country | United Kingdom |
| Sector | Public |
| PI Contribution | Culicoides nubeculosus, C. sonorensis (biting midge) and Culex pipiens (mosquito) provided as pupae for insecticide, repellent and vector competence trials as a resource. UK supplies conducted under National Capability Grant. Approximately 30 000 insects shipped during reporting period for academic users. All insect production took place at Pirbright and training in maintenance was provided to a worker from FLI (Germany). |
| Collaborator Contribution | Daughter line of C. sonorensis was established at FLI (improving security around sustaining the lines). Training and advice on insect maintenance and handling was provided by Pirbright to LSHTM, Liverpool and Reading. Studies then carried out included investigation of bacteria in Culicoides (University of Liverpool), modeling of mosquito populations (Reading) and development of novel insecticides and repellent formulations (LSHTM). These specialist expertise were driven by the host organizations for the studies. |
| Impact | Products are still in development. |
| Start Year | 2017 |
| Description | Supply of Arthropods to External Partners |
| Organisation | Bern University of Applied Sciences |
| Country | Switzerland |
| Sector | Academic/University |
| PI Contribution | Culicoides nubeculosus, C. sonorensis (biting midge) and Culex pipiens (mosquito) provided as pupae for insecticide, repellent and vector competence trials as a resource. UK supplies conducted under National Capability Grant. Approximately 30 000 insects shipped during reporting period for academic users. All insect production took place at Pirbright and training in maintenance was provided to a worker from FLI (Germany). |
| Collaborator Contribution | Daughter line of C. sonorensis was established at FLI (improving security around sustaining the lines). Training and advice on insect maintenance and handling was provided by Pirbright to LSHTM, Liverpool and Reading. Studies then carried out included investigation of bacteria in Culicoides (University of Liverpool), modeling of mosquito populations (Reading) and development of novel insecticides and repellent formulations (LSHTM). These specialist expertise were driven by the host organizations for the studies. |
| Impact | Products are still in development. |
| Start Year | 2017 |
| Description | Supply of Arthropods to External Partners |
| Organisation | Charles University |
| Country | Czech Republic |
| Sector | Academic/University |
| PI Contribution | A total of approximately 300000 biting midges and several tens of thousand mosquitoes were supplied as a national capability to receiving institutes. These were used in a wide range of studies from assessment of host location to teaching resources. The grant funds a Culicoides technician (100% FTE) and a mosquito technician (50% FTE) who are responsible for production. All use is acknowledged in papers arising and research is conducted under MTAs throughout held by the Pirbright IP department. |
| Collaborator Contribution | A range of scientific studies around biting midges and mosquitoes. For outputs see publications. |
| Impact | Both internal and external outputs have resulted in a clearer understanding of the risk of arbovirus incursion in the UK and additionally has contributed to the development of control techniques for vectors. Outputs also include outreach activities, student training and other societal and cultural collaborations. |
| Start Year | 2011 |
| Description | Supply of Arthropods to External Partners |
| Organisation | ETH Zurich |
| Country | Switzerland |
| Sector | Academic/University |
| PI Contribution | Culicoides nubeculosus, C. sonorensis (biting midge) and Culex pipiens (mosquito) provided as pupae for insecticide, repellent and vector competence trials as a resource. UK supplies conducted under National Capability Grant. Approximately 30 000 insects shipped during reporting period for academic users. All insect production took place at Pirbright and training in maintenance was provided to a worker from FLI (Germany). |
| Collaborator Contribution | Daughter line of C. sonorensis was established at FLI (improving security around sustaining the lines). Training and advice on insect maintenance and handling was provided by Pirbright to LSHTM, Liverpool and Reading. Studies then carried out included investigation of bacteria in Culicoides (University of Liverpool), modeling of mosquito populations (Reading) and development of novel insecticides and repellent formulations (LSHTM). These specialist expertise were driven by the host organizations for the studies. |
| Impact | Products are still in development. |
| Start Year | 2017 |
| Description | Supply of Arthropods to External Partners |
| Organisation | Friedrich Loeffler Institute |
| Country | Germany |
| Sector | Academic/University |
| PI Contribution | Culicoides nubeculosus, C. sonorensis (biting midge) and Culex pipiens (mosquito) provided as pupae for insecticide, repellent and vector competence trials as a resource. UK supplies conducted under National Capability Grant. Approximately 30 000 insects shipped during reporting period for academic users. All insect production took place at Pirbright and training in maintenance was provided to a worker from FLI (Germany). |
| Collaborator Contribution | Daughter line of C. sonorensis was established at FLI (improving security around sustaining the lines). Training and advice on insect maintenance and handling was provided by Pirbright to LSHTM, Liverpool and Reading. Studies then carried out included investigation of bacteria in Culicoides (University of Liverpool), modeling of mosquito populations (Reading) and development of novel insecticides and repellent formulations (LSHTM). These specialist expertise were driven by the host organizations for the studies. |
| Impact | Products are still in development. |
| Start Year | 2017 |
| Description | Supply of Arthropods to External Partners |
| Organisation | Helmholtz Association of German Research Centres |
| Department | Helmholtz Centre for Environmental Research - UFZ |
| Country | Germany |
| Sector | Academic/University |
| PI Contribution | A total of approximately 300000 biting midges and several tens of thousand mosquitoes were supplied as a national capability to receiving institutes. These were used in a wide range of studies from assessment of host location to teaching resources. The grant funds a Culicoides technician (100% FTE) and a mosquito technician (50% FTE) who are responsible for production. All use is acknowledged in papers arising and research is conducted under MTAs throughout held by the Pirbright IP department. |
| Collaborator Contribution | A range of scientific studies around biting midges and mosquitoes. For outputs see publications. |
| Impact | Both internal and external outputs have resulted in a clearer understanding of the risk of arbovirus incursion in the UK and additionally has contributed to the development of control techniques for vectors. Outputs also include outreach activities, student training and other societal and cultural collaborations. |
| Start Year | 2011 |
| Description | Supply of Arthropods to External Partners |
| Organisation | Lancaster University |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | A total of approximately 300000 biting midges and several tens of thousand mosquitoes were supplied as a national capability to receiving institutes. These were used in a wide range of studies from assessment of host location to teaching resources. The grant funds a Culicoides technician (100% FTE) and a mosquito technician (50% FTE) who are responsible for production. All use is acknowledged in papers arising and research is conducted under MTAs throughout held by the Pirbright IP department. |
| Collaborator Contribution | A range of scientific studies around biting midges and mosquitoes. For outputs see publications. |
| Impact | Both internal and external outputs have resulted in a clearer understanding of the risk of arbovirus incursion in the UK and additionally has contributed to the development of control techniques for vectors. Outputs also include outreach activities, student training and other societal and cultural collaborations. |
| Start Year | 2011 |
| Description | Supply of Arthropods to External Partners |
| Organisation | Lancaster University |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Culicoides nubeculosus, C. sonorensis (biting midge) and Culex pipiens (mosquito) provided as pupae for insecticide, repellent and vector competence trials as a resource. UK supplies conducted under National Capability Grant. Approximately 30 000 insects shipped during reporting period for academic users. All insect production took place at Pirbright and training in maintenance was provided to a worker from FLI (Germany). |
| Collaborator Contribution | Daughter line of C. sonorensis was established at FLI (improving security around sustaining the lines). Training and advice on insect maintenance and handling was provided by Pirbright to LSHTM, Liverpool and Reading. Studies then carried out included investigation of bacteria in Culicoides (University of Liverpool), modeling of mosquito populations (Reading) and development of novel insecticides and repellent formulations (LSHTM). These specialist expertise were driven by the host organizations for the studies. |
| Impact | Products are still in development. |
| Start Year | 2017 |
| Description | Supply of Arthropods to External Partners |
| Organisation | Liverpool John Moores University |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | A total of approximately 300000 biting midges and several tens of thousand mosquitoes were supplied as a national capability to receiving institutes. These were used in a wide range of studies from assessment of host location to teaching resources. The grant funds a Culicoides technician (100% FTE) and a mosquito technician (50% FTE) who are responsible for production. All use is acknowledged in papers arising and research is conducted under MTAs throughout held by the Pirbright IP department. |
| Collaborator Contribution | A range of scientific studies around biting midges and mosquitoes. For outputs see publications. |
| Impact | Both internal and external outputs have resulted in a clearer understanding of the risk of arbovirus incursion in the UK and additionally has contributed to the development of control techniques for vectors. Outputs also include outreach activities, student training and other societal and cultural collaborations. |
| Start Year | 2011 |
| Description | Supply of Arthropods to External Partners |
| Organisation | London School of Hygiene and Tropical Medicine (LSHTM) |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Culicoides nubeculosus, C. sonorensis (biting midge) and Culex pipiens (mosquito) provided as pupae for insecticide, repellent and vector competence trials as a resource. UK supplies conducted under National Capability Grant. Approximately 30 000 insects shipped during reporting period for academic users. All insect production took place at Pirbright and training in maintenance was provided to a worker from FLI (Germany). |
| Collaborator Contribution | Daughter line of C. sonorensis was established at FLI (improving security around sustaining the lines). Training and advice on insect maintenance and handling was provided by Pirbright to LSHTM, Liverpool and Reading. Studies then carried out included investigation of bacteria in Culicoides (University of Liverpool), modeling of mosquito populations (Reading) and development of novel insecticides and repellent formulations (LSHTM). These specialist expertise were driven by the host organizations for the studies. |
| Impact | Products are still in development. |
| Start Year | 2017 |
| Description | Supply of Arthropods to External Partners |
| Organisation | London School of Hygiene and Tropical Medicine (LSHTM) |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | A total of approximately 300000 biting midges and several tens of thousand mosquitoes were supplied as a national capability to receiving institutes. These were used in a wide range of studies from assessment of host location to teaching resources. The grant funds a Culicoides technician (100% FTE) and a mosquito technician (50% FTE) who are responsible for production. All use is acknowledged in papers arising and research is conducted under MTAs throughout held by the Pirbright IP department. |
| Collaborator Contribution | A range of scientific studies around biting midges and mosquitoes. For outputs see publications. |
| Impact | Both internal and external outputs have resulted in a clearer understanding of the risk of arbovirus incursion in the UK and additionally has contributed to the development of control techniques for vectors. Outputs also include outreach activities, student training and other societal and cultural collaborations. |
| Start Year | 2011 |
| Description | Supply of Arthropods to External Partners |
| Organisation | Queen's University Belfast |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Culicoides nubeculosus, C. sonorensis (biting midge) and Culex pipiens (mosquito) provided as pupae for insecticide, repellent and vector competence trials as a resource. UK supplies conducted under National Capability Grant. Approximately 30 000 insects shipped during reporting period for academic users. All insect production took place at Pirbright and training in maintenance was provided to a worker from FLI (Germany). |
| Collaborator Contribution | Daughter line of C. sonorensis was established at FLI (improving security around sustaining the lines). Training and advice on insect maintenance and handling was provided by Pirbright to LSHTM, Liverpool and Reading. Studies then carried out included investigation of bacteria in Culicoides (University of Liverpool), modeling of mosquito populations (Reading) and development of novel insecticides and repellent formulations (LSHTM). These specialist expertise were driven by the host organizations for the studies. |
| Impact | Products are still in development. |
| Start Year | 2017 |
| Description | Supply of Arthropods to External Partners |
| Organisation | Rothamsted Research |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | A total of approximately 300000 biting midges and several tens of thousand mosquitoes were supplied as a national capability to receiving institutes. These were used in a wide range of studies from assessment of host location to teaching resources. The grant funds a Culicoides technician (100% FTE) and a mosquito technician (50% FTE) who are responsible for production. All use is acknowledged in papers arising and research is conducted under MTAs throughout held by the Pirbright IP department. |
| Collaborator Contribution | A range of scientific studies around biting midges and mosquitoes. For outputs see publications. |
| Impact | Both internal and external outputs have resulted in a clearer understanding of the risk of arbovirus incursion in the UK and additionally has contributed to the development of control techniques for vectors. Outputs also include outreach activities, student training and other societal and cultural collaborations. |
| Start Year | 2011 |
| Description | Supply of Arthropods to External Partners |
| Organisation | Swedish University of Agricultural Sciences |
| Country | Sweden |
| Sector | Academic/University |
| PI Contribution | A total of approximately 300000 biting midges and several tens of thousand mosquitoes were supplied as a national capability to receiving institutes. These were used in a wide range of studies from assessment of host location to teaching resources. The grant funds a Culicoides technician (100% FTE) and a mosquito technician (50% FTE) who are responsible for production. All use is acknowledged in papers arising and research is conducted under MTAs throughout held by the Pirbright IP department. |
| Collaborator Contribution | A range of scientific studies around biting midges and mosquitoes. For outputs see publications. |
| Impact | Both internal and external outputs have resulted in a clearer understanding of the risk of arbovirus incursion in the UK and additionally has contributed to the development of control techniques for vectors. Outputs also include outreach activities, student training and other societal and cultural collaborations. |
| Start Year | 2011 |
| Description | Supply of Arthropods to External Partners |
| Organisation | Texas A&M University-Central Texas |
| Country | United States |
| Sector | Academic/University |
| PI Contribution | Culicoides nubeculosus, C. sonorensis (biting midge) and Culex pipiens (mosquito) provided as pupae for insecticide, repellent and vector competence trials as a resource. UK supplies conducted under National Capability Grant. Approximately 30 000 insects shipped during reporting period for academic users. All insect production took place at Pirbright and training in maintenance was provided to a worker from FLI (Germany). |
| Collaborator Contribution | Daughter line of C. sonorensis was established at FLI (improving security around sustaining the lines). Training and advice on insect maintenance and handling was provided by Pirbright to LSHTM, Liverpool and Reading. Studies then carried out included investigation of bacteria in Culicoides (University of Liverpool), modeling of mosquito populations (Reading) and development of novel insecticides and repellent formulations (LSHTM). These specialist expertise were driven by the host organizations for the studies. |
| Impact | Products are still in development. |
| Start Year | 2017 |
| Description | Supply of Arthropods to External Partners |
| Organisation | University of Bristol |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Culicoides nubeculosus, C. sonorensis (biting midge) and Culex pipiens (mosquito) provided as pupae for insecticide, repellent and vector competence trials as a resource. UK supplies conducted under National Capability Grant. Approximately 30 000 insects shipped during reporting period for academic users. All insect production took place at Pirbright and training in maintenance was provided to a worker from FLI (Germany). |
| Collaborator Contribution | Daughter line of C. sonorensis was established at FLI (improving security around sustaining the lines). Training and advice on insect maintenance and handling was provided by Pirbright to LSHTM, Liverpool and Reading. Studies then carried out included investigation of bacteria in Culicoides (University of Liverpool), modeling of mosquito populations (Reading) and development of novel insecticides and repellent formulations (LSHTM). These specialist expertise were driven by the host organizations for the studies. |
| Impact | Products are still in development. |
| Start Year | 2017 |
| Description | Supply of Arthropods to External Partners |
| Organisation | University of Bristol |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | A total of approximately 300000 biting midges and several tens of thousand mosquitoes were supplied as a national capability to receiving institutes. These were used in a wide range of studies from assessment of host location to teaching resources. The grant funds a Culicoides technician (100% FTE) and a mosquito technician (50% FTE) who are responsible for production. All use is acknowledged in papers arising and research is conducted under MTAs throughout held by the Pirbright IP department. |
| Collaborator Contribution | A range of scientific studies around biting midges and mosquitoes. For outputs see publications. |
| Impact | Both internal and external outputs have resulted in a clearer understanding of the risk of arbovirus incursion in the UK and additionally has contributed to the development of control techniques for vectors. Outputs also include outreach activities, student training and other societal and cultural collaborations. |
| Start Year | 2011 |
| Description | Supply of Arthropods to External Partners |
| Organisation | University of Edinburgh |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | A total of approximately 300000 biting midges and several tens of thousand mosquitoes were supplied as a national capability to receiving institutes. These were used in a wide range of studies from assessment of host location to teaching resources. The grant funds a Culicoides technician (100% FTE) and a mosquito technician (50% FTE) who are responsible for production. All use is acknowledged in papers arising and research is conducted under MTAs throughout held by the Pirbright IP department. |
| Collaborator Contribution | A range of scientific studies around biting midges and mosquitoes. For outputs see publications. |
| Impact | Both internal and external outputs have resulted in a clearer understanding of the risk of arbovirus incursion in the UK and additionally has contributed to the development of control techniques for vectors. Outputs also include outreach activities, student training and other societal and cultural collaborations. |
| Start Year | 2011 |
| Description | Supply of Arthropods to External Partners |
| Organisation | University of Greenwich |
| Department | Natural Resources Institute Greenwich |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Culicoides nubeculosus, C. sonorensis (biting midge) and Culex pipiens (mosquito) provided as pupae for insecticide, repellent and vector competence trials as a resource. UK supplies conducted under National Capability Grant. Approximately 30 000 insects shipped during reporting period for academic users. All insect production took place at Pirbright and training in maintenance was provided to a worker from FLI (Germany). |
| Collaborator Contribution | Daughter line of C. sonorensis was established at FLI (improving security around sustaining the lines). Training and advice on insect maintenance and handling was provided by Pirbright to LSHTM, Liverpool and Reading. Studies then carried out included investigation of bacteria in Culicoides (University of Liverpool), modeling of mosquito populations (Reading) and development of novel insecticides and repellent formulations (LSHTM). These specialist expertise were driven by the host organizations for the studies. |
| Impact | Products are still in development. |
| Start Year | 2017 |
| Description | Supply of Arthropods to External Partners |
| Organisation | University of Greenwich |
| Department | Natural Resources Institute Greenwich |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | A total of approximately 300000 biting midges and several tens of thousand mosquitoes were supplied as a national capability to receiving institutes. These were used in a wide range of studies from assessment of host location to teaching resources. The grant funds a Culicoides technician (100% FTE) and a mosquito technician (50% FTE) who are responsible for production. All use is acknowledged in papers arising and research is conducted under MTAs throughout held by the Pirbright IP department. |
| Collaborator Contribution | A range of scientific studies around biting midges and mosquitoes. For outputs see publications. |
| Impact | Both internal and external outputs have resulted in a clearer understanding of the risk of arbovirus incursion in the UK and additionally has contributed to the development of control techniques for vectors. Outputs also include outreach activities, student training and other societal and cultural collaborations. |
| Start Year | 2011 |
| Description | Supply of Arthropods to External Partners |
| Organisation | University of Liverpool |
| Department | School of Veterinary Science Liverpool |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Culicoides nubeculosus, C. sonorensis (biting midge) and Culex pipiens (mosquito) provided as pupae for insecticide, repellent and vector competence trials as a resource. UK supplies conducted under National Capability Grant. Approximately 30 000 insects shipped during reporting period for academic users. All insect production took place at Pirbright and training in maintenance was provided to a worker from FLI (Germany). |
| Collaborator Contribution | Daughter line of C. sonorensis was established at FLI (improving security around sustaining the lines). Training and advice on insect maintenance and handling was provided by Pirbright to LSHTM, Liverpool and Reading. Studies then carried out included investigation of bacteria in Culicoides (University of Liverpool), modeling of mosquito populations (Reading) and development of novel insecticides and repellent formulations (LSHTM). These specialist expertise were driven by the host organizations for the studies. |
| Impact | Products are still in development. |
| Start Year | 2017 |
| Description | Supply of Arthropods to External Partners |
| Organisation | University of Liverpool |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | A total of approximately 300000 biting midges and several tens of thousand mosquitoes were supplied as a national capability to receiving institutes. These were used in a wide range of studies from assessment of host location to teaching resources. The grant funds a Culicoides technician (100% FTE) and a mosquito technician (50% FTE) who are responsible for production. All use is acknowledged in papers arising and research is conducted under MTAs throughout held by the Pirbright IP department. |
| Collaborator Contribution | A range of scientific studies around biting midges and mosquitoes. For outputs see publications. |
| Impact | Both internal and external outputs have resulted in a clearer understanding of the risk of arbovirus incursion in the UK and additionally has contributed to the development of control techniques for vectors. Outputs also include outreach activities, student training and other societal and cultural collaborations. |
| Start Year | 2011 |
| Description | Supply of Arthropods to External Partners |
| Organisation | University of Lyon |
| Country | France |
| Sector | Academic/University |
| PI Contribution | A total of approximately 300000 biting midges and several tens of thousand mosquitoes were supplied as a national capability to receiving institutes. These were used in a wide range of studies from assessment of host location to teaching resources. The grant funds a Culicoides technician (100% FTE) and a mosquito technician (50% FTE) who are responsible for production. All use is acknowledged in papers arising and research is conducted under MTAs throughout held by the Pirbright IP department. |
| Collaborator Contribution | A range of scientific studies around biting midges and mosquitoes. For outputs see publications. |
| Impact | Both internal and external outputs have resulted in a clearer understanding of the risk of arbovirus incursion in the UK and additionally has contributed to the development of control techniques for vectors. Outputs also include outreach activities, student training and other societal and cultural collaborations. |
| Start Year | 2011 |
| Description | Supply of Arthropods to External Partners |
| Organisation | University of Reading |
| Department | School of Biological Sciences Reading |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Culicoides nubeculosus, C. sonorensis (biting midge) and Culex pipiens (mosquito) provided as pupae for insecticide, repellent and vector competence trials as a resource. UK supplies conducted under National Capability Grant. Approximately 30 000 insects shipped during reporting period for academic users. All insect production took place at Pirbright and training in maintenance was provided to a worker from FLI (Germany). |
| Collaborator Contribution | Daughter line of C. sonorensis was established at FLI (improving security around sustaining the lines). Training and advice on insect maintenance and handling was provided by Pirbright to LSHTM, Liverpool and Reading. Studies then carried out included investigation of bacteria in Culicoides (University of Liverpool), modeling of mosquito populations (Reading) and development of novel insecticides and repellent formulations (LSHTM). These specialist expertise were driven by the host organizations for the studies. |
| Impact | Products are still in development. |
| Start Year | 2017 |
| Description | Supply of Arthropods to External Partners |
| Organisation | University of Reading |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | A total of approximately 300000 biting midges and several tens of thousand mosquitoes were supplied as a national capability to receiving institutes. These were used in a wide range of studies from assessment of host location to teaching resources. The grant funds a Culicoides technician (100% FTE) and a mosquito technician (50% FTE) who are responsible for production. All use is acknowledged in papers arising and research is conducted under MTAs throughout held by the Pirbright IP department. |
| Collaborator Contribution | A range of scientific studies around biting midges and mosquitoes. For outputs see publications. |
| Impact | Both internal and external outputs have resulted in a clearer understanding of the risk of arbovirus incursion in the UK and additionally has contributed to the development of control techniques for vectors. Outputs also include outreach activities, student training and other societal and cultural collaborations. |
| Start Year | 2011 |
| Description | Supply of Arthropods to External Partners |
| Organisation | University of St Andrews |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | A total of approximately 300000 biting midges and several tens of thousand mosquitoes were supplied as a national capability to receiving institutes. These were used in a wide range of studies from assessment of host location to teaching resources. The grant funds a Culicoides technician (100% FTE) and a mosquito technician (50% FTE) who are responsible for production. All use is acknowledged in papers arising and research is conducted under MTAs throughout held by the Pirbright IP department. |
| Collaborator Contribution | A range of scientific studies around biting midges and mosquitoes. For outputs see publications. |
| Impact | Both internal and external outputs have resulted in a clearer understanding of the risk of arbovirus incursion in the UK and additionally has contributed to the development of control techniques for vectors. Outputs also include outreach activities, student training and other societal and cultural collaborations. |
| Start Year | 2011 |
| Description | Supply of Arthropods to External Partners |
| Organisation | University of Veterinary Medicine Hanover |
| Country | Germany |
| Sector | Academic/University |
| PI Contribution | A total of approximately 300000 biting midges and several tens of thousand mosquitoes were supplied as a national capability to receiving institutes. These were used in a wide range of studies from assessment of host location to teaching resources. The grant funds a Culicoides technician (100% FTE) and a mosquito technician (50% FTE) who are responsible for production. All use is acknowledged in papers arising and research is conducted under MTAs throughout held by the Pirbright IP department. |
| Collaborator Contribution | A range of scientific studies around biting midges and mosquitoes. For outputs see publications. |
| Impact | Both internal and external outputs have resulted in a clearer understanding of the risk of arbovirus incursion in the UK and additionally has contributed to the development of control techniques for vectors. Outputs also include outreach activities, student training and other societal and cultural collaborations. |
| Start Year | 2011 |
| Description | Supply of Arthropods to External Partners |
| Organisation | University of Zurich |
| Country | Switzerland |
| Sector | Academic/University |
| PI Contribution | A total of approximately 300000 biting midges and several tens of thousand mosquitoes were supplied as a national capability to receiving institutes. These were used in a wide range of studies from assessment of host location to teaching resources. The grant funds a Culicoides technician (100% FTE) and a mosquito technician (50% FTE) who are responsible for production. All use is acknowledged in papers arising and research is conducted under MTAs throughout held by the Pirbright IP department. |
| Collaborator Contribution | A range of scientific studies around biting midges and mosquitoes. For outputs see publications. |
| Impact | Both internal and external outputs have resulted in a clearer understanding of the risk of arbovirus incursion in the UK and additionally has contributed to the development of control techniques for vectors. Outputs also include outreach activities, student training and other societal and cultural collaborations. |
| Start Year | 2011 |
| Description | Supply of Arthropods to External Partners |
| Organisation | VUC Aarhus |
| Country | Denmark |
| Sector | Academic/University |
| PI Contribution | A total of approximately 300000 biting midges and several tens of thousand mosquitoes were supplied as a national capability to receiving institutes. These were used in a wide range of studies from assessment of host location to teaching resources. The grant funds a Culicoides technician (100% FTE) and a mosquito technician (50% FTE) who are responsible for production. All use is acknowledged in papers arising and research is conducted under MTAs throughout held by the Pirbright IP department. |
| Collaborator Contribution | A range of scientific studies around biting midges and mosquitoes. For outputs see publications. |
| Impact | Both internal and external outputs have resulted in a clearer understanding of the risk of arbovirus incursion in the UK and additionally has contributed to the development of control techniques for vectors. Outputs also include outreach activities, student training and other societal and cultural collaborations. |
| Start Year | 2011 |
| Description | Supply of Arthropods to External Partners |
| Organisation | Wageningen University & Research |
| Country | Netherlands |
| Sector | Academic/University |
| PI Contribution | Culicoides nubeculosus, C. sonorensis (biting midge) and Culex pipiens (mosquito) provided as pupae for insecticide, repellent and vector competence trials as a resource. UK supplies conducted under National Capability Grant. Approximately 30 000 insects shipped during reporting period for academic users. All insect production took place at Pirbright and training in maintenance was provided to a worker from FLI (Germany). |
| Collaborator Contribution | Daughter line of C. sonorensis was established at FLI (improving security around sustaining the lines). Training and advice on insect maintenance and handling was provided by Pirbright to LSHTM, Liverpool and Reading. Studies then carried out included investigation of bacteria in Culicoides (University of Liverpool), modeling of mosquito populations (Reading) and development of novel insecticides and repellent formulations (LSHTM). These specialist expertise were driven by the host organizations for the studies. |
| Impact | Products are still in development. |
| Start Year | 2017 |
| Description | Supply of Arthropods to External Partners |
| Organisation | Wageningen University & Research |
| Country | Netherlands |
| Sector | Academic/University |
| PI Contribution | A total of approximately 300000 biting midges and several tens of thousand mosquitoes were supplied as a national capability to receiving institutes. These were used in a wide range of studies from assessment of host location to teaching resources. The grant funds a Culicoides technician (100% FTE) and a mosquito technician (50% FTE) who are responsible for production. All use is acknowledged in papers arising and research is conducted under MTAs throughout held by the Pirbright IP department. |
| Collaborator Contribution | A range of scientific studies around biting midges and mosquitoes. For outputs see publications. |
| Impact | Both internal and external outputs have resulted in a clearer understanding of the risk of arbovirus incursion in the UK and additionally has contributed to the development of control techniques for vectors. Outputs also include outreach activities, student training and other societal and cultural collaborations. |
| Start Year | 2011 |
| Description | Supply of Arthropods to External Partners |
| Organisation | Zoological Society of London |
| Country | United Kingdom |
| Sector | Charity/Non Profit |
| PI Contribution | Culicoides nubeculosus, C. sonorensis (biting midge) and Culex pipiens (mosquito) provided as pupae for insecticide, repellent and vector competence trials as a resource. UK supplies conducted under National Capability Grant. Approximately 30 000 insects shipped during reporting period for academic users. All insect production took place at Pirbright and training in maintenance was provided to a worker from FLI (Germany). |
| Collaborator Contribution | Daughter line of C. sonorensis was established at FLI (improving security around sustaining the lines). Training and advice on insect maintenance and handling was provided by Pirbright to LSHTM, Liverpool and Reading. Studies then carried out included investigation of bacteria in Culicoides (University of Liverpool), modeling of mosquito populations (Reading) and development of novel insecticides and repellent formulations (LSHTM). These specialist expertise were driven by the host organizations for the studies. |
| Impact | Products are still in development. |
| Start Year | 2017 |
| Description | Syngenta UK |
| Organisation | Syngenta International AG |
| Department | Syngenta Ltd (Bracknell) |
| Country | United Kingdom |
| Sector | Private |
| PI Contribution | This collaboration aims to identify functional relationships between insecticide resistances in mosquitoes and competence of vector mosquitoes for arboviruses. Further, this collaboration aims at the generation of transgenic mosquitoes overexpressing proteins involved in certain insecticide resistances as tools to study the mechanism for such resistances. We have selected candidate proteins and overexpressed them in cell culture and in mosquitoes for vector competence studies as well as functional studies (insecticide uptake bioassays). |
| Collaborator Contribution | Syngenta UK has provided intellectual input into our study design and expertise in the field of insecticide resistance mechanisms. |
| Impact | No outputs yet. Collaboration between The Pirbright Institute and agrochemical industry. |
| Start Year | 2019 |
| Description | The GCRF One Health Poultry Hub |
| Organisation | Royal Veterinary College (RVC) |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | The focus of the research is to achieve sustainable global intensification of poultry meat and egg production whilst reducing risks to human and animal health and welfare. We are undertaking research to investigate the diversity of avian influenza viruses prevalent in poultry and wild birds in south east Asia. |
| Collaborator Contribution | Royal Veterinary College, London is leading this GCRF One Health Poultry Hub. The project will address the need to meet rising demand for poultry meat and eggs in developing countries, while minimising risk to international public health. Population growth is driving global demand for poultry, meat and egg production; this unfortunately creates conditions in which animal diseases can spread to humans ('zoonoses'). These include bacterial food poisoning and strains with avian influenza with epidemic or pandemic potential. The GCRF One Health Poultry Hub will adopt a 'One Health' approach to the issue of combatting animal-to-human diseases by bringing together a team of laboratory, clinical, veterinary and social scientists. This team will test and evaluate novel interventions. The need for safe poultry production is most urgent in South and South East Asia, so the RVC and its partners will then use their local networks in these regions to put its positive research to immediate use. |
| Impact | Development of international interdisciplinary partnership of 55 investigators from 13 countries to address the emerging global challenges of food security and public health associated with intensification of poultry production. |
| Start Year | 2018 |
| Description | The Pirbright "Livestock Antibody Hub" funded by Bill & Melinda Gates Foundation |
| Organisation | The Pirbright Institute |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Me and my team established collaboration in the "Livestock Antibody Hub" funded ($5.5 million) by the from Bill & Melinda Gates Foundation. Focus of our partnership is to develop techniques and capacity to analyse immune cells (B cells) of cattle, poultry and pigs that produce virus neutralizing antibodies. This research will support rational development of next generation of vaccines and to explore antibody-based passive immunization approaches for treatment and prophylaxis of infectious viral diseases affecting livestock and humans. Our initial focus is to target major animal viral pathogens that cause severe losses within Low and Middle-Income Countries (LMIC) animal production systems including avian influenza virus (AIV), Newcastle disease virus (NDV), infectious bursal disease virus (IBDV), infectious bronchitis virus (IBV) and foot-and-mouth disease virus (FMDV). |
| Collaborator Contribution | This is a collaborative project and partners are providing help in provision of reagents and techniques for analysis of diversity of B cells repertoire that produce infleunza virus-specific antibodies, single cell sequencing and bioinformatic analysis. This project will further strengthen our on-going research collaborations with many research groups in academia as well as with animal health industry (Zoetis, Boehringer Ingelheim Animal Health, Merck, CEVA, Jovac, YeBio, ILRI, Galvmed, The Roslin Institute, HuMabs, AbCellera and Distributed Bio) to take the research outputs from laboratory to the field. The outputs of research will be improved vaccines and diagnostics enabling to reduce the impact of infectious diseases on farm animals, which offer substantial direct and indirect economic, public health, environmental and social benefits to the UK and rest of the world. |
| Impact | Development of passive immunization approaches against avian influenza viruses affecting poultry (project incited in January 2020). |
| Start Year | 2020 |
| Description | Thomas Bowden - STRUBI - University of Oxford - Glycoprotein structure collaboration |
| Organisation | University of Oxford |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Provision of data and advice about the biological properties of viral glycoproteins, e.g. their fusion capacity with various receptors, their role in determining host-range |
| Collaborator Contribution | Advice and technical support at the structural level supporting future project development and design |
| Impact | No outcomes as yet |
| Start Year | 2018 |
| Description | Trevor Sweeney - University of Cambridge PhD Studentship |
| Organisation | University of Cambridge |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Development of PhD studentship project in collaboration with Dr. Sweeney's lab at Cambridge and application at Pirbright. |
| Collaborator Contribution | Development of PhD studentship project and application at Cambridge. |
| Impact | The PhD studentship has been funded and is currently been advertised. |
| Start Year | 2018 |
| Description | University of Liverpool Proteomics Collaboration |
| Organisation | University of Liverpool |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Generation of samples to analyse the membranous proteome of cells infected with paramyxoviruses. |
| Collaborator Contribution | Proteomic analysis of samples and technical support. Access to previous data-sets and expertise. |
| Impact | Preliminary data sets only to date. |
| Start Year | 2018 |
| Description | Use of fluorescent virions to isolate PRRSV-specific porcine B cells |
| Organisation | University of Minnesota |
| Country | United States |
| Sector | Academic/University |
| PI Contribution | Deployment of a novel technology to facilitate isolation of PRRSV neutralizing monoclonal antibodies |
| Collaborator Contribution | Provision of a method to fluorescently tag purified virions to label PRRSV-specific B cells |
| Impact | None to report to date |
| Start Year | 2016 |
| Description | Wilhelm Gerner |
| Organisation | University of Veterinary Medicine Vienna |
| Country | Austria |
| Sector | Academic/University |
| PI Contribution | Our lab hosted Dr Gerner who brought samples for RNA-seq. My lab planned and executed the sequencing and is contributing to the analysis. |
| Collaborator Contribution | Dr Gerner did all the animal work and cell sorting to produce the samples reading for RNA-seq |
| Impact | None as yet, analysis is ongoing |
| Start Year | 2019 |
| Description | Y chromosome-linked X-shredding gene drives |
| Organisation | Hebrew University of Jerusalem |
| Country | Israel |
| Sector | Academic/University |
| PI Contribution | We shared with the partner Y chromosome-linked docking lines generated in our lab. |
| Collaborator Contribution | Provided some plasmid constructs. |
| Impact | No outputs yet. |
| Start Year | 2021 |
| Description | York |
| Organisation | University of York |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Collaboration |
| Collaborator Contribution | Collaboration |
| Impact | Collaboration |
| Start Year | 2015 |
| Title | PPR DIVA vaccine |
| Description | We ahve developed two PPR live attenuated DIVA vaccines that can differentiate between vaccinated and infected animals. |
| IP Reference | PCT/GB2019/053641,WO2020128496 |
| Protection | Patent application published |
| Year Protection Granted | 2020 |
| Licensed | Commercial In Confidence |
| Impact | Till date there is no PPR vaccine avalable that can differentiate between vaccination and infection. This causes a huge issue on eradication of the didease and declare freedom from the disease. Therefore our newly develped chimeric live attenauted PPR vaccine and DIVA tests can differentiate between vaccination and infection which is a great achievement for ongoing PPR eradication. Please see detail from the below web. https://patentscope.wipo.int/search/en/detail.jsf?docId=WO2020128496 |
| Title | CARGO: effective format-free compressed storage of genomic information |
| Description | CARGO is a compressor compiler. Given a high-level description of a file format (such as FASTQ or SAM) and compression methods for each field, it automatically generates optimized (de)compressors for such a format. Random access and range queries are provided for sorted datasets. Arbitrary sets of CARGO-compressed files can be stored together in a CARGO container. |
| Type Of Technology | Software |
| Year Produced | 2016 |
| Impact | Technical solutions derived from CARGO are being discussed for incorporation into the upcoming MPEG (Moving Picture Experts Group) standard for the compression of genomic information |
| Description | Poster presentation: International Pig Veterinary Symposium and International PRRS Symposium, Chongqing, PR China |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Luke Johnson (PhD student) gave a poster presentation on his studies on the interaction of macrophages and dendritic cells with PRRSV of differing virulence. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Talk Presented at 2nd International Congress of Veterinary Microbiology, 16th -19th October 2018. Sherwood Breezes Resort-Lara, Antalya, Turkey. |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Conference discussed improving prevention and control of viral diseases of poultry. |
| Year(s) Of Engagement Activity | 2018 |
| Description | "Challenges for Poultry Industry". Organised by Fakieh Poultry at the Ritz-Carlton Hotel in Jeddah, Saudi Arabia, 2nd -3rd December 2018, |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Conference discussed prevention and control of infectious diseases affecting poultry production. |
| Year(s) Of Engagement Activity | 2018 |
| Description | "Interactive session with poultry stakeholders". Title: Overview of Avian Influenza group Research at The Pirbright Institute. Presented at University of Veterinary and Animal Sciences (UVAS) Lahore, Pakistan, 3rd August 2018. |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | The workshop discussed the economic impacts and prevention strategies against avian influenza viruses affecting poultry production and zoonotic infections. |
| Year(s) Of Engagement Activity | 2018 |
| Description | 1st Research Coordination Meeting (RCM) of the Coordinated Research Project (CRP) on "Generic approach for the development of genetic sexing strains for SIT applications" Vienna, 7-11 October 2019 |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | FAO/IAEA Coordinated Research Project on exploring genetic and molecular methods of sex separation in insect pests; aimed at promoting specific areas of research, exchange of ideas and networking among the experts in the field. |
| Year(s) Of Engagement Activity | 2019 |
| Description | 2. Overview of Avian Influenza group Research at The Pirbright Institute. Presented at "Interactive session with poultry stakeholders" at University of Veterinary and Animal Sciences (UVAS) Lahore, Pakistan, 3rd August 2018. |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Dissemination of research Project outcomes These include presenting research data for vaccine seed strains with emergent field avian influenza variants; The zoonotic risk posed by emerging avian influenza viruses circulating in the wild birds and poultry. Impact of virus evolution on vaccine efficacy and persistence in poultry. |
| Year(s) Of Engagement Activity | 2018 |
| Description | 360 degree Lab Animal Tour (and film) of The Plowright Building, The Philip Mellor Insectary, and Animal Isolation Facilities |
| Form Of Engagement Activity | Engagement focused website, blog or social media channel |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Public/other audiences |
| Results and Impact | The website provides unique access to the animal research facilities at The Pirbright Institute which support its world-leading scientific research into livestock viral diseases and helps save countless animal and potentially human lives, globally. Produced and filmed by Understanding Animal Research, the Lab Animal Tour was filmed at Pirbright, and at three other research centres, and provides the viewer with an interactive 360 virtual tour of the animal facilities. |
| Year(s) Of Engagement Activity | 2017 |
| URL | http://www.labanimaltour.org/pirbright |
| Description | 7th European Veterinary Immunology Workshop |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Selected oral presentation: Developing a 'One Health' Nipah virus vaccine to protect animal and public health |
| Year(s) Of Engagement Activity | 2021 |
| Description | 7th European Veterinary Immunology Workshop - William Mwangi |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | I was tasked with reading abstracts, inviting speakers, organising the timetable and moderating the VETERINARY IMMUNOLOGY TOOLKIT session as a co-chair. |
| Year(s) Of Engagement Activity | 2021 |
| URL | https://www.7theviw.org |
| Description | A talk at a symposium entitled: Using the 3Rs to support good science |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | I gave a talk at a Symposium held at The Pirbright Institute entitled: "Using the 3Rs to support good science". The talk was entitled: "A chicken primary B cell culture model to study the pathogenesis and improve the control of immunosuppressive viruses of poultry" |
| Year(s) Of Engagement Activity | 2017 |
| Description | A talk at the British Poultry Diseases Group meeting |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Industry/Business |
| Results and Impact | The Poultry Diseases Group meet quarterly and is comprised of representatives from private veterinary practices, vaccine and pharmaceutical industries, and the Animal and Plant Health Agency (APHA). I gave a talk at one of the meetings outlining my research to date and future directions. |
| Year(s) Of Engagement Activity | 2017 |
| Description | AHSV talk on transmission for OIE (Thailand online) |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Talk on transmission of AHSV to interested parties following outbreak in 2020. Talk given on 9/6/20 to approx 150. |
| Year(s) Of Engagement Activity | 2020 |
| Description | AN INTERVIEW WITH PROFESSOR VENUGOPAL NAIR |
| Form Of Engagement Activity | Engagement focused website, blog or social media channel |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Postgraduate students |
| Results and Impact | Professor Venugopal Nair is a Research Group Leader at The Pirbright Institute, a visiting Professor of Avian Virology at the Department of Zoology, and a Jenner Investigator at the Jenner Institute, University of Oxford. He is also a member of the Microbiology Society, and in this interview, he tells us more about his research into viral diseases of livestock. |
| Year(s) Of Engagement Activity | 2020 |
| URL | https://microbiologysociety.org/membership/meet-our-members/focus-area-viruses/an-interview-with-pro... |
| Description | Amin Asfor presented the findigs of the project at the Microbiology Society Annual meeting, 2019 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Amin Asfor presented the findigs of the project at the Microbiology Society Annual meeting, 2019. Title: in vivo and ex vivo models of Infectious Bursal Disease Virus (IBDV) in inbred chicken lines differing in their resistance to the disease. |
| Year(s) Of Engagement Activity | 2019 |
| Description | Amin Asfor presented the work at the NC3Rs Fellows Day |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | Amin Asfor presented a poster at the NC3Rs Fellows Day entitled: Chicken primary B cell culture model to study the pathogenesis and improve the control of immunosuppressive viruses of poultry |
| Year(s) Of Engagement Activity | 2019 |
| Description | Article in Microscopy & Analysis - ELMI, Joanna Wells |
| Form Of Engagement Activity | A magazine, newsletter or online publication |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Joanna wrote a summary of the ELMI meeting as an article for the publication. |
| Year(s) Of Engagement Activity | 2021 |
| Description | Ash Manor GCSE Mentoring 2018 - BA |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | Stimulating increased interest in research |
| Year(s) Of Engagement Activity | 2018 |
| Description | Attend SEMT |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Attend in person conference, Society for Electron Microscopy Technology |
| Year(s) Of Engagement Activity | 2021 |
| Description | Attend SEMT - Joanna Wells |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Engaged with microscopy community |
| Year(s) Of Engagement Activity | 2021 |
| Description | Avian Influenza H9N2 and H7N9 evolution, fitness in poultry and zoonotic potential presented by Munir Iqbal at UC DAVIS EDUCATION CONFERENCE ON ONE HEALTH FOR FOOD SAFETY, AGRICULTURE, AND ANIMAL HEALTH |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | The research was discussed with academic and non-academic participants the risks of avian influenza viruses currently circulating in poultry and carry potential to cause zoonotic infections. Since avian influenzas viruses (AIVs) exist in many subtypes and co-infection of two or more different AIV subtypes in an individual bird can lead to the generation of novel reassortant viruses. The emergent reassortant viruses may carry differential phenotypic characteristics (virulence, host-transmission and host-range) compared to their parental viruses. Our studies revealed that experimental co-infection of chickens with two different subtypes of avian influenza viruses (H9N2 and H7N9) led to the emergence of novel reassortant H9N9 viruses which carry greater virulence for poultry and an increased zoonotic and pandemic potential. Our results also provided evidence that both H9N2 and H7N9 viruses can rapidly acquire antigenic changes in vaccinated birds causing vaccine failure and cocirculation of these viruses can also rapidly lead to rapid generation of novel reassortment viruses with increased virulence and host-range posing threat to both animals and humans. This presentation increased awareness among the audience risk posed by co-circulation of different subtypes of avian influenza viruses in poultry and wild birds and how best we can detect emerging threats and develop the disease risks and disease mitigation strategies. |
| Year(s) Of Engagement Activity | 2021 |
| URL | https://www.wifss.ucdavis.edu/onehealthsummer21/ |
| Description | Avian influenza virus evolution impacts on virulence and antigenicity |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Postgraduate students |
| Results and Impact | A talk was presented at Royal Veterinary College, London, by Thusitha Karunarathna (a PhD student in the Avian Influenza group at The Pirbright Institute). The Talk described the mechanisms used by the avian influenza viruses to evade host immunity to cause disease outbreaks and persists in poultry. |
| Year(s) Of Engagement Activity | 2021 |
| Description | Avian influenza viruses can combine two techniques to evade vaccine immunity |
| Form Of Engagement Activity | Engagement focused website, blog or social media channel |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Media (as a channel to the public) |
| Results and Impact | A study by researchers at The Pirbright Institute reveals more clues about how avian influenza viruses can evade vaccine immunity, aiding their spread in the field. The findings are published in Emerging Microbes and Infections. Influenza viruses are able to mutate, which can enable them to evade immunity generated by natural infection or vaccination. Influenza viruses have four main ways of duping the immune system so that they can continue to infect and spread between birds. Pirbright scientists investigated two of these to further understand how the H9N2 avian influenza virus can change its genetic makeup to overcome poultry vaccines. One of the methods the virus can use is to alter one of its surface proteins, haemagglutinin (HA), which enables the virus to enter the cell and replicate. HA is also a common target for the immune system's antibodies, which block the virus from binding to cell receptors. By changing just one or two protein components, HA can latch on far more tightly to the cell, preventing antibodies from stopping the virus. However, this can come at a cost, as particularly strong binding can prevent effective replication. The other method scientists investigated is how H9N2 viruses disguise themselves by adding sugar chains to the surface of their HA proteins. This can block antibodies from binding, but also has varying fitness outcomes for the influenza virus. The researchers found that the location of the sugar chain on HA could determine how effectively the virus replicated. They also demonstrated that the two evasive techniques could either compliment or act against each other - if an influenza virus had gained mutations that enable it to bind more strongly to cells, a sugar chain in the right position could restore its ability to replicate effectively. In contrast, weakly binding influenza viruses do not benefit from HA sugar additions, which instead reduce the virus's ability to replicate. Professor Munir Iqbal, head of the Avian Influenza Group at Pirbright said, "We can use this knowledge to develop new vaccines that will help antibodies to recognise how the flu virus can change. This will provide protection to birds even as the virus evolves. We can also use this information to understand how viruses survive in poultry despite vaccination and monitor new adaptions that may appear in the field." This research was funded by the Biotechnology and Biological Sciences Research Council (BBSRC), part of UK Research and Innovation (UKRI) and the British Council with support from the Newton Fund. Article: Sealy, J. E., Peacock, T. P., Sadeyen, J. R., Chang, P., Everest, H. J., Bhat, S., Iqbal, M. (2020). Adsorptive mutation and N-linked glycosylation modulate influenza virus antigenicity and fitness. Emerging Microbes & Infections, advance online publication, 12 November 2020, doi: 10.1080/22221751.2020.1850180 [SOURCE: The Pirbright Institute] |
| Year(s) Of Engagement Activity | 2020 |
| URL | https://www.star-idaz.net/2020/12/avian-influenza-viruses-can-combine-two-techniques-to-evade-vaccin... |
| Description | Avian influenza viruses in Bangladesh, Vietnam, and Sri Lanka: investigating genotype to phenotype (antigenicity, virulence, host-range). Webinar on 28th March 2021. |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Presented talk by Munir iqbal describing ongoing research outcomes. We identified genetic determined that modules avian influenza H5 and H9 antigenicity virulence and transmission fitness in different host species. The role of identified markers that change the virus binding to host cells and impact on virus-host interaction leading to mild or severe disease manifestation in chickens and mammalian species (humans). |
| Year(s) Of Engagement Activity | 2021 |
| Description | Avian influenza: tackling large numbers of outbreaks this winter in the UK |
| Form Of Engagement Activity | A magazine, newsletter or online publication |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Public/other audiences |
| Results and Impact | Nearly two years on, the COVID-19 pandemic is still dominating the news and continues to cause concern for populations across the globe. However, UK poultry and wild bird populations are currently facing their own health threat. We are not the only ones that have faced lockdowns because of spreading viruses, UK poultry and captive birds have also faced a similar situation to protect them from avian influenza. Scientists at The Pirbright Institute are working to increase understanding of how genetic changes in influenza A viruses can impact disease spread, host responses to infection and the pathology associated with the disease. What is the avian influenza virus? Avian influenza viruses are Influenza A viruses that circulate and transmit amongst birds. The disease is what we commonly know as 'bird flu'. Influenza A viruses can infect a range of hosts, including humans and two subtypes H1N1 and H3N2 circulate as seasonal flu each winter in the UK. Avian influenza viruses are different to those that can infect humans in that they cannot easily infect humans and must change and adapt to do so. Aquatic birds are a natural reservoir for avian influenza viruses, these birds typically experience fewer clinical symptoms but can spread disease to domestic birds, particularly in the migratory season, between October and March. Avian influenza viruses are categorised as either highly pathogenic (HPAI) or low pathogenic (LPAI) and this categorisation is linked to the severity of disease in chickens. HPAI has a high mortality rate which can be up to 100%, other symptoms can include swelling of the head, death of cells in the comb and wattle, red discolouration of legs and feet and diarrhoea. HPAI causes disease in more organs than just those in the respiratory or gastrointestinal tracts like LPAI, which mostly presents as respiratory symptoms such as a snick (like a sneeze), a rattle in the chest (known as rales) and discharge from the eyes and nose. What is causing bird flu outbreaks in domestic birds now? We are currently in the normal migratory season of wild birds. In the spring and summer months, these birds spend their time breeding and raising their young in North-eastern Asia where they mix with other bird species which enables virus transmission and mutation. Then, when they migrate for the autumn and winter months, they shed virus in their faeces along the way. One of the migratory paths is across Northern Europe and this includes the UK. This year, in the UK, the first avian influenza outbreak was on 15 October and was discovered in captive aquatic birds. To date (17 December 2021) there have been 50 outbreaks in England, 1 in Wales and 2 in Scotland, this has resulted in over half a million birds being destroyed to control the spread of disease. Are there more outbreaks this year compared to other years? The number of outbreaks this year has been the most extensive ever. There have been over 50 outbreaks in the UK, compared to 26 outbreaks last year. Although it is hard to pin down the reason for this high number, we know it is not linked to the COVID-19 pandemic. The UK's response to the spread of avian influenza has been rapid, and from 29 November birds were required to be housed inside with additional biosecurity measures put in place such as foot dips and disinfecting clothing. Testing and surveillance of wild birds have shown that there is a high prevalence of the virus in these birds currently, and this is contributing to the spread of disease to farm and domestic bird populations. A more detailed look at the genetic make-up of these viruses shows that they are different to those from previous years. This is predicted to be as a result of the virus infecting multiple birds across a range of species, and this provides the perfect opportunity for the virus to evolve. Analysis of these genetic changes does not raise any alarm bells for increased transmission to humans, but they are likely impacting the spread and severity of disease in birds. What should we do if we find a dead bird or are concerned about bird health? If the public finds dead waterfowl birds they are encouraged not to touch them but to report them to Defra using the appropriate phone number that can be found on the website, also any other wild birds found dead in numbers of five or greater should be reported. What is Pirbright doing to tackle this virus? Researchers at Pirbright are attempting to enhance the control and detection of avian influenza in poultry populations as well as extend our basic knowledge about the virus in avian and mammalian hosts. The research groups led by Professor Munir Iqbal and Dr Holy Shelton are investigating how avian influenza virus strains originated from wild birds can rapidly adapt to cause disease outbreaks in poultry and gain the potential to infect humans. In addition, Professor Munir Iqbal is developing improved poultry vaccines that prevent virus replication and spread. These vaccines are particularly useful in countries where avian influenza is regularly found in the domestic poultry and the control and prevention of spread isn't easy, such as in the UK. |
| Year(s) Of Engagement Activity | 2021 |
| URL | https://www.pirbright.ac.uk/news/2021/12/avian-influenza-tackling-large-numbers-outbreaks-winter-uk |
| Description | BBC Today programme 24/01/2019 |
| Form Of Engagement Activity | A press release, press conference or response to a media enquiry/interview |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Media (as a channel to the public) |
| Results and Impact | Interview on BBC Today programme regarding potential development and use of gene drives in mice |
| Year(s) Of Engagement Activity | 2019 |
| URL | https://www.bbc.co.uk/sounds/play/m00025bt |
| Description | BBC4 Big and Small series of programmes filmed 2021 |
| Form Of Engagement Activity | A broadcast e.g. TV/radio/film/podcast (other than news/press) |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Public/other audiences |
| Results and Impact | Filming for section on electron microscopy of viruses. Programme not due to be scheduled until Spring 2022 |
| Year(s) Of Engagement Activity | 2021 |
| Description | BBSRC Partnering Seminar, University of Liverpool, Tamilnadu Veterinary and Animal Science University |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Talk presented entitled " Molecular determinants of antigenicity of H7 and H9 avian Influenza viruses". The research data shared with the colleagues working to reduce the impact of avian infectious diseases on poultry production. The discussion led to more collaboration and work together, acquiring reagents and protocols for further research and development activities. |
| Year(s) Of Engagement Activity | 2019 |
| Description | BSI report on the future of veterinary immunology and vaccinology |
| Form Of Engagement Activity | A magazine, newsletter or online publication |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Other audiences |
| Results and Impact | Organised and published a joint IVVN/BSI report to raise awareness of the UK's research status in veterinary vaccinology and immunology and the importance of maintaining this for the R&D landscape 'Securing Our Future: the value of veterinary vaccines'. This was aimed at influencing policymakers to support and commit to maintaining the UK's leading position. |
| Year(s) Of Engagement Activity | 2021 |
| Description | BSI webinar |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Other audiences |
| Results and Impact | I presented a webinar hosted by the British Society for Immunology entitled "BSI Coronavirus webinar: What can we learn from the animal coronaviruses?", which sparked questions and discussions afterwards. I have since been contacted to establish new collaborations with people in the audience. |
| Year(s) Of Engagement Activity | 2020 |
| Description | Big Band Science Event- AH, ER, LVC, MdP |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Public/other audiences |
| Results and Impact | Stimulating increased interest in science and research |
| Year(s) Of Engagement Activity | 2019 |
| Description | Big Bang Fair South East - RC |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Public/other audiences |
| Results and Impact | Regional science festival/event - changes in public attitudes and stimulating increased interest in research |
| Year(s) Of Engagement Activity | 2018 |
| Description | Big Bang SE |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Public/other audiences |
| Results and Impact | Participation (HB and MA) in Big Bang SE |
| Year(s) Of Engagement Activity | 2019 |
| URL | https://www.thebigbangfair.co.uk/ |
| Description | Big Bang UK- Jennifer Simpson |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Schools |
| Results and Impact | Big Bang UK is a science competition. JS acted as moderator for final projects submitted by students from across the UK. |
| Year(s) Of Engagement Activity | Pre-2006,2019 |
| Description | Bird flu study from Pirbright Institute sheds light on mutations and health impacts |
| Form Of Engagement Activity | A press release, press conference or response to a media enquiry/interview |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Media (as a channel to the public) |
| Results and Impact | Bird flu study from Pirbright Institute sheds light on mutations and health impacts. Emerging research from the Pirbright Institute shows that avian influenza mutations could increase disease threat to poultry, but make it less infectious for humans. Mutations in the H7N9 bird flu virus increase its replication rate and stability in avian cells, but reduce its preference for infecting human cells, a study from The Pirbright Institute has found. The results show that outbreaks in birds caused by strains with these mutations could pose a lower risk to human health, while remaining a significant threat to the poultry industry. H7N9 low-pathogenicity avian influenza (LPAI) virus usually infects birds, but in 2013 the first human case was recorded in China. Since then, there have been over 1,500 confirmed human infections with about 40 percent of cases resulting in fatalities. Further evolution of the H7N9 virus gave rise to a high-pathogenicity avian influenza (HPAI) strain that could cause 100 percent mortality in chickens. In 2017 the Chinese Government initiated a wide scale vaccination programme in chickens which drove down cases of both low and high pathogenicity strains. Previous Pirbright research identified three mutations in avian influenza H7N9 viruses that enabled them to overcome immunity generated by vaccines. The mutations altered a protein on the outside of the virus called haemagglutinin (HA), which binds to host cell receptors and allows the virus to enter and cause infection. In the latest study, published in the Journal of Virology, the team discovered the same mutations occurred in H7N9 viruses isolated from the field in 2019, which most likely emerged in birds that had either been immunised or naturally infected. Analysis of the viruses demonstrated that strains with these mutations have significantly increased replication rates in both chicken cell cultures and chick embryos as well as displaying greater acid and thermal stability, which could improve transmission ability. The mutations also influenced which animal cells the strain could enter - their HA proteins could still bind to the receptors of bird cells, but they lost their affinity for human cell receptors. These findings show that in evolving to escape the chicken immune system, the mutated H7N9 viruses have reduced their risk to human health whilst increasing the threat they pose to poultry, although further studies in birds are needed to confirm the impact this could have on infection and disease spread. Professor Munir Iqbal, head of the Avian Influenza Group at Pirbright, said: "Our approach has allowed us to accurately predict avian influenza mutations that appear in the field, and assess how these mutations could affect the risk they pose to both human and avian populations. This information can be used to inform surveillance efforts and provide early warnings of potentially dangerous emerging strains." |
| Year(s) Of Engagement Activity | 2020 |
| URL | https://www.thepoultrysite.com/news/2020/07/bird-flu-study-from-pirbright-institute-sheds-light-on-m... |
| Description | Blenheim High School Fair - DB |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | Team members attended sessions at a local high school with secondary school children and those in the 6th form to talk about paths into scientific careers, sharing their experiences of working at The Pirbright Institute. Approximately 300 students attended and it stimulated increased interest in science and research. |
| Year(s) Of Engagement Activity | 2019 |
| Description | Bluetongue and Wildlife (15 minute presentation on Epizootic Haemorhagic Disease Virus), (July/Pirbright, UK) |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Policymakers/politicians |
| Results and Impact | Policymakers reported greater interest in deer-associated diseases including EHDV and BTV |
| Year(s) Of Engagement Activity | 2018 |
| Description | Brownies-AH |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | Talking to the Brownies about science and their careers |
| Year(s) Of Engagement Activity | 2019 |
| Description | Bull/Intel press release |
| Form Of Engagement Activity | A press release, press conference or response to a media enquiry/interview |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | The Integrative Biology and Bioinformatics group at Pirbright has acquired a bespoke computer cluster for genomics from Atos/Bull. Paolo Ribeca and Bryan Charleston were asked by Atos/Bull and Intel to contribute to a case study that illustrates the usefulness of high-performance computing in combating animal diseases and improving livestock welfare. |
| Year(s) Of Engagement Activity | 2017 |
| URL | http://globenewswire.com/news-release/2017/01/10/904852/0/en/The-Pirbright-Institute-chooses-an-Atos... |
| Description | CC Careers Fair |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | MA participated in a careers fair at Collingwood College |
| Year(s) Of Engagement Activity | 2019 |
| Description | CNN interview |
| Form Of Engagement Activity | A press release, press conference or response to a media enquiry/interview |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Media (as a channel to the public) |
| Results and Impact | Interview with CNN journalist. Journalist later followed up asking me to check and clarify some issues. |
| Year(s) Of Engagement Activity | 2019 |
| Description | Camberley Library Fun Palace 2018 - JM |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Public/other audiences |
| Results and Impact | Changes in public attitudes and stimulating increased interest in research |
| Year(s) Of Engagement Activity | 2018 |
| Description | Career Development and Training Focused Interest Group 2021 |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Policymakers/politicians |
| Results and Impact | Royal Microscopical Society (international membership) FIG, training and career development for microscopists |
| Year(s) Of Engagement Activity | 2021 |
| Description | Centre of excellence for research on avian diseases (CERAD) |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Other audiences |
| Results and Impact | I was invited to present my work at the UK-China Centre of excellence for research on avian diseases (CERAD) meeting attended by researchers from UK, China and Thailand. There was lots of discussion about the research and future directions, including potential collaborations. |
| Year(s) Of Engagement Activity | 2017 |
| Description | Chair - Royal Entomological Society Session on Insect Vectors |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | Chaired session on Insect Vectors including selecting speakers and managing session. |
| Year(s) Of Engagement Activity | 2019 |
| URL | https://www.royensoc.co.uk/sites/default/files/RES%20Ento%20'19%20Reg.%20Leaflet_web.pdf |
| Description | Chair BioimagingUK meeting 2021 |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Discussion on funding issues influencing equipment acquisition in UK |
| Year(s) Of Engagement Activity | 2021 |
| Description | Chair and Co-Organiser for UK Vector-borne Diseases Conference |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | UK Vector-borne Diseases meeting held bi-Annually. Half day sessions on 2nd, 9th, 16th, 23rd November 2020. Chaired session on 9th November 2020. Meeting was held online with invited and selected presentations and a poster session. Also included funders from Defra and BBSRC. |
| Year(s) Of Engagement Activity | 2020 |
| Description | Cheltenham Science Festival |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Public/other audiences |
| Results and Impact | Stimulating increased interest in science and research |
| Year(s) Of Engagement Activity | 2019 |
| Description | Cheltenham Science Festival |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Public/other audiences |
| Results and Impact | Institute stand at the festival covering the Institute science in general, and transmission and gene editing to control disease, in particular. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Cheltenham Science Festival 2018 - JA |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Public/other audiences |
| Results and Impact | Major science festival - changes in public attitudes and stimulating increased interest in research |
| Year(s) Of Engagement Activity | 2018 |
| Description | Cheltenham Science Festival 2018 - JM |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Public/other audiences |
| Results and Impact | Major science festival - changes in public attitudes and stimulating increased interest in research |
| Year(s) Of Engagement Activity | 2018 |
| Description | Cheltenham Science Festival 2018 - JP |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Public/other audiences |
| Results and Impact | Major national science festival - changes in public attitudes and stimulating increased interest in research |
| Year(s) Of Engagement Activity | 2018 |
| Description | Cheltenham Science Festival 2018 - RC |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Public/other audiences |
| Results and Impact | Major science festival - changes in public attitudes and stimulating increased interest in research |
| Year(s) Of Engagement Activity | 2018 |
| Description | Cheltenham Science Festival 2018 - SV |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Public/other audiences |
| Results and Impact | Display at major science festival: changes in public attitudes and stimulating increased interest in research |
| Year(s) Of Engagement Activity | 2018 |
| Description | Cheltenham Science Festival 2018-DP |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Public/other audiences |
| Results and Impact | Major national science festival - changes in public attitudes and stimulating increased interest in research |
| Year(s) Of Engagement Activity | 2018 |
| Description | Cheltenham Science Festival- AH, ER, JC |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Public/other audiences |
| Results and Impact | Present at the Pirbright stand to discuss science with the public |
| Year(s) Of Engagement Activity | 2019 |
| Description | Cheltenham Science festival - LB |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Public/other audiences |
| Results and Impact | National science festival which Team members attended to demonstrate to the public how scientists at The Pirbright Institute can work to contain and control viral outbreaks. Over 500 people accessed the information and this stimulated increased interest in science and research. |
| Year(s) Of Engagement Activity | 2019 |
| Description | Cheltenhan Science Festival - Dana Perry |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Public/other audiences |
| Results and Impact | DP helped to run a stand at the festival, questions from the general public stimulated increased interest in science and research and increased the visibility of The Pirbright Institute. |
| Year(s) Of Engagement Activity | 2019 |
| Description | Commentary on AHSV outbreak in Thailand in Science Magazine |
| Form Of Engagement Activity | A magazine, newsletter or online publication |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Media (as a channel to the public) |
| Results and Impact | 'Thailand scrambles to contain major outbreak of horse-killing virus' - Article in Science Mag April 2020 |
| Year(s) Of Engagement Activity | 2020 |
| URL | https://www.sciencemag.org/news/2020/04/thailand-scrambles-contain-major-outbreak-horse-killing-viru... |
| Description | Conference session organiser at MMC2017 - Host Pathogen Interactions |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Chair for the Imaging Host-Pathogen Interactions session run at MMC2017 (and in previous years). Session usually attracts approx. 30 delegates from national/international backgrounds, mostly academic. Talks sparked discussions and networking opportunities. |
| Year(s) Of Engagement Activity | 2012,2014,2015,2017 |
| Description | Copenhagen PhD course |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Postgraduate students |
| Results and Impact | Scientific organiser and lecturer on PhD course in EM at University of Copenhagen |
| Year(s) Of Engagement Activity | 2021 |
| Description | Coronavirus lecture MSc students (Surrey) |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Postgraduate students |
| Results and Impact | A lecture was presented on the replication, cellular interactions and pathogenesis of coronaviruses to 15-20 MSc students from University of Surrey. The students were engaged and interested, answering and asking questions during the session. |
| Year(s) Of Engagement Activity | 2016,2018 |
| Description | DARPA visit April 2017 |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Supporters |
| Results and Impact | Hosted visit by US funding agency programme manager to The Pirbright Institute |
| Year(s) Of Engagement Activity | 2017 |
| Description | Deimante Lukosaityte: Microbiology Society conference 2019. Poster presentation: Chicken protection against H9N2 virus by passive immunization |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Described techniques for development of next generation of vaccines against viral diseases affecting animals and humans. |
| Year(s) Of Engagement Activity | 2019 |
| Description | Demonstrating Mathematics (Angita Shrestha) |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Postgraduate students |
| Results and Impact | Angita Shrestha (PhD student within Avian Influenza group) provided help in a Maths session to the new Oxford Doctoral Training Porgramme (DTP) cohort. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Determinants of antigenicity of H9 Avian Influenza Viruses. UK-China Swine and Poultry Workshop.17-18 June 2019, The Pirbright Institute, UK. |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | The talk and the discussion on the improvement of disease control systems (vaccines and diagnostics) to prevent avian influenza viruses infection in poultry and zoonotic infections in humans. |
| Year(s) Of Engagement Activity | 2019 |
| Description | Diamond Light Source Open day - HJM |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Public/other audiences |
| Results and Impact | A public open day for Diamond Light Source, which Pirbright attended as a user of the facility to showcase research being performed at Pirbgith in collaboration with Diamond. There was a lot of interest in the fundamental research and sicussion about future plans and how it fit with the facility at Diamond. |
| Year(s) Of Engagement Activity | 2019 |
| Description | Diego Friere Pesquisa Fapesp |
| Form Of Engagement Activity | A press release, press conference or response to a media enquiry/interview |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Media (as a channel to the public) |
| Results and Impact | Interview with journalist leading to article regarding release of transgenic mosquitoes into the environment to control dengue and other diseases |
| Year(s) Of Engagement Activity | 2019 |
| URL | https://revistapesquisa.fapesp.br/en/2020/02/05/jacobina-controversy/ |
| Description | Discuss gene drives with NZ Parliamentary Commissioner |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Policymakers/politicians |
| Results and Impact | Met NZ Parliamentary Commissioner to provide background information on, and discuss, gene drive systems. New Zealand is considering genetic and other control methods for invasive pests. |
| Year(s) Of Engagement Activity | 2018 |
| Description | EM-UK |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | EM-UK scientific organiser |
| Year(s) Of Engagement Activity | 2020 |
| Description | EM-UK |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | Co-organiser and speaker for UKRI supported network for electron microscopists in the UK. I was one (or two) of the founder members. Meeting has been running for 5 years. Increased the visibility of The Pirbright Institute. |
| Year(s) Of Engagement Activity | 2020 |
| Description | EM-UK - Joanna Wells |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Build network within the microscopy community |
| Year(s) Of Engagement Activity | 2021 |
| Description | EM-UK 2021 |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | Scientific organiser, meeting for EM community in UK |
| Year(s) Of Engagement Activity | 2021 |
| Description | EM-UK 2022 |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Scientific organiser of EM-UK |
| Year(s) Of Engagement Activity | 2022 |
| Description | EM-UK: A national network for electron microscopists |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Founder and Chair of EM-UK, a national network (with international participants) for electron microscopists. Includes a one or two day meeting with invited speakers, and email discussion list for questions, comments, advertising courses, advertising jobs. It has proved very popular with over 200 people registering for the discussion list, and 90+ delegates at each meeting. Administration is handled by the Royal Microscopical Society. |
| Year(s) Of Engagement Activity | 2016,2017,2018 |
| Description | EMBO Workshop: Molecular and population biology of mosquitoes and other disease vectors: vector and disease control |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Presentation on "Sex determination pathway in Anopheles gambiae as a target for mosquito control" given during the meeting held on 22 - 26 July 2019 in Kolymbari, Greece. Following a presentation a request was made to give an informal seminar at the Department of Life Sciences, Imperial College, London. |
| Year(s) Of Engagement Activity | 2019 |
| URL | https://meetings.embo.org/event/19-mosquitoes |
| Description | Encouraging women into science and engineering STEM. |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | MH Visited Warwick School, Redhill to talk to secondary students about careers in STEM. A very positive outcome and well received. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Engineered Recombinant Single Chain Variable Fragment of Monoclonal Antibody Provides Protection to Chickens Infected with H9N2 Avian Influenza. Presented by Deimante Lukosaityte at Influenza Update meeting 4th - 15th December 2020: The University of Liverpool (online meeting) |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | The talk describes a technique for the development of immunotherapy to protect poultry from Infectious diseases such as avian influenza viruses. The talk provided evidence that recombinant antibodies can provide passive immunity to protect chickens from avian influenza. Several mAbs generated from mice hybridomas targeting hemagglutinin (HA) of H9N2 virus were converted into single-chain variable fragment (scFv) antibodies. Recombinant scFvs were purified from insect cell culture supernatants and showed it could retain neutralization capacity against H9N2 virus in vitro. To test therapeutic scFv efficacy in vivo we selected two of the recombinantly produced scFv antibodies for prophylaxis and treatment of chickens infected with H9N2 UDL-01 virus. Intranasally treated birds showed reduced disease manifestation and virus shedding. These results indicate that antibody therapy could be beneficial for the provision of rapid treatment for reducing disease impacts in poultry. |
| Year(s) Of Engagement Activity | 2020 |
| URL | https://www.gla.ac.uk/researchinstitutes/iii/cvr/events/influenza/ |
| Description | Epizone participation by team member |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Postgraduate students |
| Results and Impact | Postdocteral scientist attended poster session at EPIZONE, "Breaking walls" Berlin 2019 |
| Year(s) Of Engagement Activity | 2019 |
| Description | European Society Vector Ecology (Invited Chair for two sessions and speaker for 15min talk); (October/Palermo, Sicily) |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Chaired two sessions, one on vector-borne diseases, one on scientific networks. Latter included a discussion with early career researchers. Main outcome was coordination between active networks including The Gnatwork. |
| Year(s) Of Engagement Activity | 2018 |
| URL | http://www.sove.org/European%20SOVE%20folder/greecescientificprogram/palermo_Scientific_program_revi... |
| Description | Evolution of H9N2 avian influenza virus under immune pressure. Presented at 10th International Symposium on Avian Influenza, 15th - 18th April 2018, The Grand Hotel, Brighton, UK. |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | The primary aim of the workshop was to forge long-term research partnerships between early-career researchers, livestock industry and national disease control authorities in the UK and the Philippines. In addition, the activity hopes provide information about the emerging and next generation diagnostic and vaccine strategies and their utilization to reduce the impact of viral diseases on livestock and poultry. |
| Year(s) Of Engagement Activity | 2019 |
| URL | https://www.slideshare.net/zerep_cire/2019-newton-agham-researcher-links-workshop-vaccines-and-diagn... |
| Description | Expert commentary on New Scientist article |
| Form Of Engagement Activity | A magazine, newsletter or online publication |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Public/other audiences |
| Results and Impact | I provided expert commentary on an article published in New Scientist, which summarised research performed by researchers at the University of Leeds on how mosquito bites enhance the transmission of vector-borne diseases. |
| Year(s) Of Engagement Activity | 2020 |
| URL | https://institutions.newscientist.com/article/2231141-skin-cream-applied-to-mosquito-bites-stops-vir... |
| Description | Farnborough Sixth Form College Careers Fair - Chris Chiu |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | CC engaged at a careers day, and answered questions presented by sixth form students about working in science. Increased the visibility of The Pirbright Institute. |
| Year(s) Of Engagement Activity | 2019 |
| Description | Farnborough futures careers event - HJM |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Schools |
| Results and Impact | The careers event aimed to introduce the range of careers available at Pirbright. In addition I discussed my own career progression and highlighted the range of possible careers that I could have entered. Finally, I discussed some details of the research with interested students. The students had a wide range of backgrounds and career aims but were interested to hear about the Institute and the sort of work opportunities available. Several of them planned to follow up by investigating future job opportunities at Pirbright. |
| Year(s) Of Engagement Activity | 2019 |
| Description | Gene Drive Resarch Sponsors and Supporter's Forum meeting Montreal Sept 2018 |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Supporters |
| Results and Impact | Forum met to discuss the state of the art and future considerations for gene drive research. Ongoing deliberations including specific follow-up meetings. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Genetic engineering speeds up poultry vaccine development |
| Form Of Engagement Activity | A press release, press conference or response to a media enquiry/interview |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Media (as a channel to the public) |
| Results and Impact | Researchers report the rapid generation of a bivalent vaccine against Marek's disease and avian influenza Scientists at The Pirbright Institute have used genetic engineering to develop a vaccine that protects birds against both Marek's disease and avian influenza. They say the process is faster and more efficient compared to previous methods, which will allow poultry vaccine producers to rapidly update vaccine strains in order to keep up with virus evolution. In a study published in the journal Vaccines, the team used the gene editing tool CRISPR/Cas9 to insert an avian influenza gene into the turkey herpesvirus (HVT) that is used in Marek's disease vaccines. The gene codes for haemagglutinin (HA), an influenza protein that is key for virus entry into host cells and is therefore an important target of the host immune response to block infection. "HVT is already widely used in vaccines against Marek's disease and can be administered to eggs, enabling automated delivery and providing birds with protection from the day they hatch. The additional HA gene we have incorporated will be expressed by HVT when it replicates in host cells, meaning that a single dose of vaccine induces immunity against both viruses", explained Professor Munir Iqbal, Head of the Avian Influenza group at Pirbright. "The genetic engineering process we have developed using CRISPR/Cas9 is far quicker and more efficient than previous technologies, as well as being very consistent and accurate. These attributes are essential for providing fast and reliable vaccine production to protect poultry", Prof Iqbal added. Mutations can occur with high frequency in HA proteins, enabling influenza viruses to evade immunity generated by vaccines. Using CRISPR/Cas9 to create new vaccines will help tackle evolving viruses by reducing the time it takes manufacturing companies to adapt their vaccine strains to match these mutations, allowing faster responses to outbreaks and providing better protection. Although this gene editing method is very reliable, a small number of HVT viruses fail to incorporate the HA gene into their own genome effectively. To ensure HA is being expressed in the vaccine, the team exploited the HA protein's ability to bind to molecules on the surface of red blood cells. Chicken cells infected with the successfully modified HVT virus express HA on their surface which red blood cells attach to, forming a clump that can be visualised using microscopy. This simple test improves the speed at which suitable vaccine candidates can be identified and has a much broader application as it can also be used for other viruses that incorporate proteins which interact with red blood cells. "Improving how we make vaccines is critical for preventing avian influenza outbreaks. Our work could help reduce the spread of disease between birds and reduce the risk of infection for people who work closely with poultry", Prof Iqbal said. "Now that we have developed a rapid method for generating this modified vaccine, our next steps will be to study the vaccine's effectiveness in field trials." |
| Year(s) Of Engagement Activity | 2020 |
| URL | https://www.cabi.org/vetmedresource/news/66612 |
| Description | Gordon's schools careers Fair-AH |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | Provoking interest in a range of science career paths |
| Year(s) Of Engagement Activity | 2020 |
| Description | Guest lecturer MSc Imaging course at University of Copenhagen |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Postgraduate students |
| Results and Impact | 25 internal students from University of Copenhagen visited the core imaging facility for three days during which time I delivered lectures and interacted with the students during practical workshops. This sparked questions and discussion relating to their individual projects. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Guest lecturer for PhD course in EM, University of Copenhagen |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Postgraduate students |
| Results and Impact | Workshop for delegates including lectures, seminars and practical sessions. Outcomes are that delegates are able to make informed decisions regarding microscopy techniques about their future projects, and perform sample preparation and operate instruments. Delegates could ask me for advice regarding their projects which sparked questions and discussion within the group on a wide range of multi-disciplinary subjects. Increase visibility of The Pirbright Institute |
| Year(s) Of Engagement Activity | 2019 |
| Description | H9N2 avian influenza viruses: impact of evolutionary changes on virus antigenicity, receptor binding and zoonotic potential. Presented at "Conference on Animal Infectious Diseases and Human Health" held at The Veterinary Biotechnology Branch of Chinese Association of Animal Science and Veterinary Medicine & the Veterinary Immunology Branch of Chinese Society for Immunology. August 7th-10th, 2018 Harbin, China. |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Postgraduate students |
| Results and Impact | Focus of the conference was to improve control against infectious diseases affecting poultry and livestock. |
| Year(s) Of Engagement Activity | 2018 |
| Description | H9N2 avian influenza viruses: impact of evolutionary changes on virus antigenicity, receptor binding and zoonotic potential. Presented at "Conference on Animal Infectious Diseases and Human Health" held at The Veterinary Biotechnology Branch of Chinese Association of Animal Science and Veterinary Medicine & the Veterinary Immunology Branch of Chinese Society for Immunology. August 7th-10th, 2018 Harbin, China. |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Dissemination of research outcomes to prevent and control of infectious diseases of animals and humans. - Avian influenza virus evolution and efficacy. - |
| Year(s) Of Engagement Activity | 2018 |
| Description | High pathogenicity avian Influenza (HPAI) H5 in Europe -Epidemiology and Surveillance. Presented via Webinar, organized by Boehringer Ingelheim, 3rd July 2020. |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Talk entitled; High pathogenicity avian Influenza (HPAI) H5 in Europe -Epidemiology and Surveillance. talk was organized by Boehringer Ingelheim, 3rd July 2020 and presented via Webinar. |
| Year(s) Of Engagement Activity | 2020 |
| Description | Holt School Surrey. Bee meadow & A-level outreach Talk. |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | I discussed the importance of pollinators for biodiversity and food security with the younger students. I also discussed careers in science with A-Level students. There was good interaction and many questions surrounding both topics. |
| Year(s) Of Engagement Activity | 2018 |
| Description | IUCN Technical Subgroup on Synthetic Biology and Biodiversity Conservation (meeting 12-15 April and report) |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Policymakers/politicians |
| Results and Impact | Participated as member in Technical Subgroup on Synthetic Biology and Biodiversity Conservation of International Union for Conservation of Nature (IUCN). Meetings, online discussion and ultimately a report to Task Force and will ultimately go to IUCN General Assembly. |
| Year(s) Of Engagement Activity | 2018,2019 |
| Description | Identification of antigenic epitopes to broaden and enhance the efficacy of avian influenza vaccines. Presented at 6th Animal Vaccines and immune Adjuvant Technologies Salon & Advanced seminar, Shandong-Binzhou Animal Science and Veterinary Medicine Academy, China, 18-20 October 2019. |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | The talk and the discussion was the best practices to improve control systems (vaccines and diagnostics) for prevention of avian influenza viruses infection in poultry and zoonotic infections in humans. |
| Year(s) Of Engagement Activity | 2019 |
| Description | Imagineering Fair |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Public/other audiences |
| Results and Impact | Talked to the general public about Pirbright science and Beekeeping |
| Year(s) Of Engagement Activity | 2019 |
| Description | Immuno-pathobiology of H9N2 avian influenza viruses: looking at how the viruses evolve and persist in poultry. Presented at "Techniques for Healthy Farming and Diseases Prevention & Control of Livestock and Poultry. Beijing, August 13th-15th, 2018. |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Postgraduate students |
| Results and Impact | Dissemination of research outputs. Improvement of vaccines and diagnostics. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Improvement in the Production Systems and Potency of Poultry Vaccines. Virtual meeting organised by Hi-Tech Poultry Breeders (Pvt) Ltd, Lahore, Pakistan. 17th January 2022 |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | A talk entitled "Improvement in the Production Systems and Potency of Poultry Vaccines" was presented by Munir Iqbal to veterinary field staff working on poultry production, particularly prevention and control of infectious diseases. The Audience was very much impressed with the new improved technology that enhance the potency of poultry vaccines. |
| Year(s) Of Engagement Activity | 2022 |
| Description | Improving Poultry Vaccines, Virtual presentation to staff and postgraduate students at University of Veterinary and Animal Sciences, Lahore, Pakistan |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Postgraduate students |
| Results and Impact | The overview was presented on the development of next-generation of poultry vaccines. The new vaccine technology selectively targets chicken immune cells and induces faster and stronger immunity against poultry viral diseases. A huge interest in the subject was perceived and everyone was interested in the new technology and eager in suggesting that this vaccine should be taken forward for commercial production and field use. |
| Year(s) Of Engagement Activity | 2022 |
| Description | Improving vaccines and diagnostics for Avian influenza viruses affecting poultry. Presented at the International Poultry Expo "Poultry Science Conference", Lahore, Pakistan, 13-15 September 2019. |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | The talk and the discussion was the best practices to improve control systems (vaccines and diagnostics) for prevention of avian influenza viruses infection in poultry and zoonotic infections in humans. |
| Year(s) Of Engagement Activity | 2019 |
| Description | InFocus article (Jennifer Simpson) |
| Form Of Engagement Activity | A magazine, newsletter or online publication |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | InFocus review article for EM-UK meeting (2017) |
| Year(s) Of Engagement Activity | 2017 |
| Description | Influenza antibodies reduce disease in chickens |
| Form Of Engagement Activity | A magazine, newsletter or online publication |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Public/other audiences |
| Results and Impact | Research outputs were mass comminated for the general public via a news story. cientists have engineered synthetic antibody molecules that can be administered to poultry to reduce the symptoms of influenza. The molecules also decrease the amount of influenza virus shed into the environment, suggesting there is potential for their use as immunotherapy treatments to reduce the burdens of influenza in poultry. The research, conducted by a team at the Pirbright Institute, Surrey, UK, involved generating synthetic antibodies against the H9N2 strain of avian influenza, which poses significant risks to both the poultry sector and wider avian and mammalian species due to its ability to adapt to new hosts through mutation. The modified antibody segments, known as single chain variable fragment antibodies (scFvs), were shown to prevent the H9N2 virus from entering cells in laboratory tissue cultures. Further testing showed that chickens immunised intranasally with scFvs produced lower levels of virus and presented decreased weight loss compared to those that had not received treatment. The scFvs antibodies work by binding to proteins on the outside of the influenza virus, called haemagglutinin (HA), that attach to receptors on host cells. The prevents the virus from entering the cell and replicating, offering rapid protection to infected birds. To create the scFVs, the team immunised mice with an influenza vaccine, which triggered the production of mouse antibodies that could neutralise the H9N2 virus. The genetic code for 2 section of the antibodies that bind to the HA protein was then sequenced. Using genetic engineering, they linked these 2 sections together to create one new smaller antibody molecule (the scFvs). By generating these smaller molecules, the team was able to overcome issues presented by using whole antibodies, such as lower production rates and increased likelihood of immune rejection by species other than chickens. These results are encouraging and demonstrate that scFvs could provide a quick and efficient way of reducing the clinical signs of influenza and its spread in infected flocks, reducing the risk of transmission from poultry to humans." - Professor Munir Iqbal Published in Vaccines, the scFvs created in this study have additional advantages including their ability to be mass-produced in insect cells and the fact they could work against the virus in all hosts without adaption. Commenting on the results, Professor Munir Iqbal, head of the avian influenza group at Pirbright, said: "These results are encouraging and demonstrate that scFvs could provide a quick and efficient way of reducing the clinical signs of influenza and its spread in infected flocks, reducing the risk of transmission from poultry to humans. "The next step will be to establish the best method for administration, whether this is intranasal, as we have done in this study, or via aerosol or viral vector delivery systems." Prof Iqbal added that the type of immunotherapy could be used to treat other viral diseases of poultry and humans. |
| Year(s) Of Engagement Activity | 2020 |
| URL | https://www.poultryworld.net/health-nutrition/influenza-antibodies-reduce-disease-in-chickens/ |
| Description | Influenza update meeting (Pengxiang Chang) |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | presented work entitled: Antigenic characterization of avian influenza H7N9 virus by in vitro immune escape mutant selection method. |
| Year(s) Of Engagement Activity | 2018 |
| Description | InnovSur : Conference in IRD Montpellier on Insect Vector Surveillance |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Working group on standardisation of techniques for surveillance of insect vectors. Included break out group brainstorming and subsequent commentaries. Total audience approximately 110 people for main lectures. |
| Year(s) Of Engagement Activity | 2018 |
| URL | https://www.alphavisa.com/isessah-innovsur/2018/ |
| Description | Innovate Guildford |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Public/other audiences |
| Results and Impact | Institute stand at Innovate Guildford science festival. Visitors to stand able to discuss issues related to animal diseases and their control (with a particular focus on influenza and on use of genetic modification technologies) |
| Year(s) Of Engagement Activity | 2017 |
| Description | Innovate Guildford (Angita Shrestha): |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Public/other audiences |
| Results and Impact | GENERAL SCIENCE |
| Year(s) Of Engagement Activity | 2019 |
| Description | Innovate Guildford - DB & KC |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Public/other audiences |
| Results and Impact | Public science event where team members participated on a Pirbright Institute stand which contained activities to help the public understand how scientists can help to contain and control viral outbreaks. Over 500 members of the general public attended which stimulated increased interest in science and research and led to requests for more information. |
| Year(s) Of Engagement Activity | 2019 |
| Description | Innovate Guildford 17 March 2018 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Public/other audiences |
| Results and Impact | Talk and exhibit at Innovate Guildford - >100 for talk, probably >500 for exhibit. Both sparked questions and discussion afterwards. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Innovate Guildford 2018 |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Public/other audiences |
| Results and Impact | Institute stand at the Innovate Guildford event, covering the work of the Institute in general and genetic modification through gene editing specifically. |
| Year(s) Of Engagement Activity | 2018 |
| URL | https://www.guildford.gov.uk/IG18 |
| Description | Innovate Guildford 2018 - SB |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Public/other audiences |
| Results and Impact | Regional science festival - changes in public attitudes and stimulating increased interest in research |
| Year(s) Of Engagement Activity | 2018 |
| Description | Innovate Guildford 2018 - SV |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Public/other audiences |
| Results and Impact | Display at regional science/innovation festival - changes in public attitudes and stimulating increased interest in research |
| Year(s) Of Engagement Activity | 2018 |
| Description | Innovate Guildford, ND |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Public/other audiences |
| Results and Impact | Presented at the Pirbright Institute stand discussing science performed by the Institute with the general public. The stand focussed on an exhibit on avian influenza virus but more general virology topics were also discussed including our research. |
| Year(s) Of Engagement Activity | 2017 |
| Description | Innovate Guilford-AH, AA, ER, EL |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Public/other audiences |
| Results and Impact | General science festival engaging the public in the research undertaken at Pirbright |
| Year(s) Of Engagement Activity | 2019 |
| Description | Institute stand at Surrey Vet School open day |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Schools |
| Results and Impact | Stimulating increased interest in science and research |
| Year(s) Of Engagement Activity | 2019 |
| Description | Interactions between receptor-binding, immune evasion and glycan shielding. Presented by Joshua Sealy at Biophysics and evolution: improving models to predict influenza vaccine effectiveness) |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | Influenza viruses have an error-prone polymerase complex that facilitates a mutagenic environment. Antigenic mutants swiftly arise from this environment with the capacity to persist in both humans and economically important livestock even in the face of vaccination. Furthermore, influenza viruses can adjust the antigenicity of the haemagglutinin (HA) protein, the primary influenza immunogen, using one of four molecular mechanisms. Two prominent mechanisms are: (1) enhancing binding avidity of HA toward cellular receptors to outcompete antibody binding and (2) amino acid substitutions that introduce an N-linked glycan on HA that sterically block antibody binding. In this paper, we investigate the impact that adsorptive mutation and N-linked glycosylation have on receptor-binding phenotype, virus replicative fitness and antigenicity. We show that in the context of adsorptive mutation that N-linked glycosylation can mitigate virus attenuation. We show that in the absence of adsorptive mutation, N-linked glycosylation can attenuate virus this indicating a cooperative role between adsorptive mutation and N-linked glycosylation. |
| Year(s) Of Engagement Activity | 2021 |
| URL | https://www.physicsoflife.org.uk/biophysics-and-evolution.html |
| Description | International Avian Respiratory Disease Conference |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | Michael presented his research at the 2018 International Avian Respiratory Disease Conference in the US to an audience of avian researchers and representatives form the poultry veterinary vaccine industry. This sparked discussion and potential future collaborations |
| Year(s) Of Engagement Activity | 2018 |
| Description | International Day of Women and Girls in Science |
| Form Of Engagement Activity | Engagement focused website, blog or social media channel |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Public/other audiences |
| Results and Impact | I gave a quote as a STEM ambassador about why I think it is important to make sure women and girls have the same opportunities in STEM. This was shared on social media to celebrate International Day of Women and Girls in Science, a UN-led initiative. |
| Year(s) Of Engagement Activity | 2019 |
| Description | Interview with Associated Press |
| Form Of Engagement Activity | A press release, press conference or response to a media enquiry/interview |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Media (as a channel to the public) |
| Results and Impact | Interview with a journalist from Associated Press about coronavirus research at The Pirbright Institute. |
| Year(s) Of Engagement Activity | 2020 |
| Description | Interview with BBC Radio Surrey |
| Form Of Engagement Activity | A press release, press conference or response to a media enquiry/interview |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Media (as a channel to the public) |
| Results and Impact | Interview with journalists from BBC Radio Surrey on the breakfast show about coronavirus research at The Pirbright Institute. |
| Year(s) Of Engagement Activity | 2020 |
| Description | Interview with BuzzFeed News |
| Form Of Engagement Activity | A press release, press conference or response to a media enquiry/interview |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Media (as a channel to the public) |
| Results and Impact | Interview with a journalist from BuzzFeed News about coronavirus research at The Pirbright Institute. |
| Year(s) Of Engagement Activity | 2020 |
| Description | Interviews to discuss COVID vaccine results |
| Form Of Engagement Activity | A press release, press conference or response to a media enquiry/interview |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Public/other audiences |
| Results and Impact | Radio interviews (BBC Radio Surrey and Oxford), television interview (BBC South Today) and interviews with press to discuss results from our studies evaluating COVID-19 vaccine candidates in pigs. |
| Year(s) Of Engagement Activity | 2020,2021 |
| Description | Investigating avian influenza antigenic evolution and improving potency of poultry vaccines (Internal seminar) |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Professional Practitioners |
| Results and Impact | A talk presented describing how the genetic evolution of avian influenza viruses can result in vaccine failure and how we developed approaches that improve the effectiveness of poultry vaccines to reduce the economic loss in poultry. We describe our research data H5 subtype of avian influenza viruses are evolving rapidly and being classified into different clades. These viruses carry significant antigenic heterogeneity and a single candidate vaccine may not be able to protect against the virus variants infecting poultry in different countries around the globe. Our data will allow for the generation of vaccines that are better strain-matched, thus reducing the impact of AIVs in the poultry industry. We also present our work on the development of next-generation poultry vaccines termed "Targeted Antigen Delivery Vaccine (TADV)" that selectively deliver vaccine antigens to the chicken immune cells known as Antigen-presenting cells and potentiates immunogenicity of the vaccine. |
| Year(s) Of Engagement Activity | 2022 |