Functional characterization of phytoplasma effector proteins and the role of salivary gland proteins in plant colonization of aphids
Lead Research Organisation:
John Innes Centre
Department Name: Contracts Office
Abstract
The goal of this project is to identify and characterize plant pathogen and pest virulence (effector) proteins that manipulate plants to become better hosts for these pathogens and pests. We have established genome sequence annotation pipelines to identify candidate effector proteins from phytoplasmas (bacterial plant pathogen transmitted by phloem-feeding leafhoppers) and aphids (phloem-feeding insects that transmit a variety of plant diseases).
Some phytoplasmas, including Aster Yellows strain Witches’ Broom (AY-WB), have broad plant host ranges. We have identified several effector proteins that target plant cell nuclei and change plant morphology comparable to that of AY-WB-infected plants. These morphological changes are beneficial for the leafhopper vector, which is required for dissemination of phytoplasmas in nature. The effector protein genes are encoded on potential mobile units (PMUs) that are abundant in the genomes of phytoplasmas and appear to have had major influence on phytoplasma evolution and diversification. Some PMUs are predominantly expressed during AY-WB infection of plants and others in insects. This project will lead to the functional characterization of at least one AY-WB effector.
The majority of plants are resistant against the majority of aphid species, and only some aphid species, such as the generalist Myzus persicae, can feed from multiple plant host species. Insects release a variety of proteins into the plant phloem in their saliva. These proteins can benefit the insect by reprogramming plant development, but may also play a role in plant resistance to insect attack. This project will focus on aphid interactions with Arabidopsis and crop plants. The project will use the expanding genomics resources for Myzus spp. and Arabidopsis and the JIC infrastructure resources to better understand aphid-plant interactions. The aphid salivary proteome will be investigated and the impact of some secreted proteins on plant responses determined.
Some phytoplasmas, including Aster Yellows strain Witches’ Broom (AY-WB), have broad plant host ranges. We have identified several effector proteins that target plant cell nuclei and change plant morphology comparable to that of AY-WB-infected plants. These morphological changes are beneficial for the leafhopper vector, which is required for dissemination of phytoplasmas in nature. The effector protein genes are encoded on potential mobile units (PMUs) that are abundant in the genomes of phytoplasmas and appear to have had major influence on phytoplasma evolution and diversification. Some PMUs are predominantly expressed during AY-WB infection of plants and others in insects. This project will lead to the functional characterization of at least one AY-WB effector.
The majority of plants are resistant against the majority of aphid species, and only some aphid species, such as the generalist Myzus persicae, can feed from multiple plant host species. Insects release a variety of proteins into the plant phloem in their saliva. These proteins can benefit the insect by reprogramming plant development, but may also play a role in plant resistance to insect attack. This project will focus on aphid interactions with Arabidopsis and crop plants. The project will use the expanding genomics resources for Myzus spp. and Arabidopsis and the JIC infrastructure resources to better understand aphid-plant interactions. The aphid salivary proteome will be investigated and the impact of some secreted proteins on plant responses determined.
Organisations
- John Innes Centre, United Kingdom (Collaboration, Lead Research Organisation)
- Royal Horticultural Society, United Kingdom (Collaboration)
- Wageningen University, Netherlands (Collaboration)
- Chinese Academy of Sciences (Collaboration)
- Sultan Qaboos University (Collaboration)
- Academia Sinica (Collaboration)
- University of Liverpool, United Kingdom (Collaboration)
- Natural Environment Research Council (Collaboration)
- Forest Research, United Kingdom (Collaboration)
- University of Lisbon (Collaboration)
- SESVanderhave (Collaboration)
- University of Wisconsin Madison, United States (Collaboration)
- University of Stirling, United Kingdom (Collaboration)
- Earlham Institute, NORWICH (Collaboration)
- Chinese Academy of Agricultural Sciences (Collaboration)
- Fera Science Limited, LONDON (Collaboration)
- University of East Anglia, United Kingdom (Collaboration)
- Stellenbosch University, South Africa (Collaboration)
- Institute of Plant Protection, National Research Institute (Collaboration)
- Kyoto University, Japan (Collaboration)
- Department for Environment, Food and Rural Affairs (Defra) (Collaboration)
- East Malling Research, United Kingdom (Collaboration)
- University of Zagreb (Collaboration)
- Syngenta International AG (Collaboration)
- Science and Advice for Scottish Agriculture (Collaboration)
- University of Salford, United Kingdom (Collaboration)
Publications
Ammar El-D
(2011)
Spiroplasma-like organisms closely associated with the gut in five leafhopper species (Hemiptera: Cicadellidae).
in Archives of microbiology
Ammar El-D
(2009)
Cellular and molecular aspects of rhabdovirus interactions with insect and plant hosts.
in Annual review of entomology
Bai X
(2009)
Transcriptomic analysis of the entomopathogenic nematode Heterorhabditis bacteriophora TTO1.
in BMC genomics
Bai X
(2009)
AY-WB phytoplasma secretes a protein that targets plant cell nuclei.
in Molecular plant-microbe interactions : MPMI
Bos JI
(2010)
A functional genomics approach identifies candidate effectors from the aphid species Myzus persicae (green peach aphid).
in PLoS genetics
Cañizares M
(2015)
Arabidopsis thaliana , an experimental host for tomato yellow leaf curl disease-associated begomoviruses by agroinoculation and whitefly transmission
in Plant Pathology
Chang HH
(2015)
Complete Genome Sequence of "Candidatus Sulcia muelleri" ML, an Obligate Nutritional Symbiont of Maize Leafhopper (Dalbulus maidis).
in Genome announcements
Coleman AD
(2015)
Persistence and transgenerational effect of plant-mediated RNAi in aphids.
in Journal of experimental botany
Coleman AD
(2014)
Silencing of aphid genes by feeding on stable transgenic Arabidopsis thaliana.
in Methods in molecular biology (Clifton, N.J.)
| Title | Video Abstract for Genome Biology publication |
| Description | Genome Biology created a video abstract to explain the science described in the Genome Biology to students. The video shows animation of an aphid changing plant hosts with a narrator who explains what happens in the aphid upon the host change. The video was posted on YouTube. |
| Type Of Art | Film/Video/Animation |
| Year Produced | 2017 |
| Impact | So far the video had 488 views on YouTube, and 9 viewers liked the video and none disliked the video. The video has likely increased the amount of attention the Genome Biology article received by different media (current Altmetric attention score of the paper is 110). |
| URL | https://www.youtube.com/watch?v=EKy_SuaFKMk |
| Title | Video showing how aphids sense aphid attack |
| Description | We launched a short video showing the method by which we can monitor the calcium elevations that occur in a leaf during aphid probing. |
| Type Of Art | Film/Video/Animation |
| Year Produced | 2018 |
| Impact | The video will be understood by a wide audience. |
| URL | https://www.youtube.com/watch?v=CK2iIPfcnvo |
| Description | The funding in this project has been used to generate preliminary data for obtaining additional funding. It enabled us to continue projects between funding cycles and start new ones. We generated these data for two systems. One system is focused on the obligate bacterial pathogen Phytoplasma, which is transmitted by sap-feeding insects. The other system is focused on aphids, which are sap-feeding insects that transmit a diverse range of economically important plant pathogens, such as viruses. For aphids, we generated genome, transcriptome and proteome data that led to the discovery of effector proteins that aphids use to modulate plant processes and successfully colonize plants. We found that the effector proteins are present in the saliva of aphids and that these proteins are inoculated into plants. One effector was located in the cytoplasm of plant cells (Mugford et al., 2017. MPMI). We found that this effector suppresses plant defense responses (Bos et al., 2010. PLoS Genetics; Drurey et al., 2018. Biorxiv). We generated evidence that this effector is conserved among sap-feeding insects (Drurey et al., 2018. Biorxiv) and elucidated candidate plant targets. These findings were communicated to colleagues of the sugarbeet breeding company SESVanderHave who directly funded or co-funded (via a BBSCR-LINK) further research on aphid-plant interactions that includes functional analyses of other aphid effectors and their targets. We used funds in this project to develop the RNA interference (RNAi) technology to knock down gene expression in aphids. We showed that we can knock down gene expression in aphids via plant-mediated RNAi (Pitino et al., 2013. PLoS One; Coleman et al., 2015. JXB). We also generated a first genome assembly and annotation of the green peach aphid Myzus persicae in collaboration with David Swarbreck at TGAC (now the Earlham Institute). These data led to collaborations with Syngenta Ltd via a BBSRC-IPA grant that is focused on using aphid genome sequence information and RNAi for studying pesticide resistance and plant host adjustment mechanisms in aphids. This collaboration with Syngenta has progressed very well and led to a second BBSRC-IPA grant that started last year. Syngenta is using our findings to inform their strategies to develop RNAi-mediated pest control technologies. The research on RNAi also provided preliminary data to further develop the RNAi technology for whitefly that was funded by the Bill and Melinda Gates Foundation. We also used funding from this research project to investigate how plants respond to aphid feeding. We found that plants have specific receptors that detect aphid elicitors leading to a specific plant defense response in the plant (Prince et al., 2014. Plant Physiol.). We identified an aphid effector that suppresses this plant defense response (Mugford et al., 2017. MPMI; Drurey et al., 2018. Biorxiv). This work instigated collaborations with colleagues at the John Innes Centre and the University of Madison, Wisconsin, USA, and the funding of PhD studentship to further investigate the nature of the plant defense response. We developed methods to detect local calcium bursts that surround the aphid feeding site and identified plant factors that regulate these calcium elevations (Vincent et al., 2017. Plant Cell). The work resulted in a successful PhD student thesis and defense and is taken further by two PhD students in the lab. One of these students won a prestigious Gatsby fellowship to fund his PhD. The other PhD student has initiated collaborations with colleagues at The Sainsbury Laboratory and at Kyoto University, Japan. Therefore, this project has led to collaborations with colleagues within the JIC and at other research centers in Norwich, USA and in Japan. The funds also contributed to research that identified and characterized effectors from Phytoplasma. Phytoplasma colonize their host plants and insect vectors. We found that 65% of the Phytoplasma effector genes are upregulated when these bacteria colonize the host plant, whereas 35% of the effector genes are upregulated in the insect vector (MacLean et al., 2011. Plant Physiol). This project contributed to research that identified plant targets for some of Phytoplasma effectors that are upregulated in the plant (Sugio et al., 2011. PNAS; Sugio et al., 2014. New Phytol; MacLean et al., PLoS Biol) and to the demonstration that the effectors suppress plant defense responses to the insect vectors (Sugio et al., 2011. PNAS; MacLean et al., 204; Orlovskis et al., 2016. Front. Biotic Interact.). Based on these data we were successfull at winning a BBSRC-FAPESP grant and the prestigous Human Frontier Science Program (HFSP) research grant, and a Marie Curie fellowship (to Allyson MacLean) and a Newton fellowship (to Adi Kliot). The BBSRC-FAPESP grant was a collaboration with colleagues in Brazil on the maize-pathogenic Maize bushy stunt phytoplasma. A PhD student in my lab visited my collaborator in Brazil and we published part of this research (Orlovkis et al., 2017. Annals Botany). The HFSP grant is for an interdisciplinary project with collaborators in the USA, Poland, The Netherlands and the UK, and involves mechanistic modelling, molecular and biochemistry research, insect assays and field-based work. The writing of several papers to report the outputs of this research is ongoing. |
| Exploitation Route | Our findings are taken forward by academic research laboratories worldwide, including collaborators mentioned above. Our industrial partners are using our data to improve pest control strategies and develop crops that are more resistant to insects. Undergraduate, MSc and PhD students have successfully graduated and are pursuing careers, mostly in science, in the UK and wordwide. Several former postdoctoral researchers are leading their own research laboratories in the UK, France and Canada. |
| Sectors | Agriculture, Food and Drink,Chemicals,Education,Environment |
| Description | The research findings discovered in this project provided preliminary data used for obtaining research funding from various sources, including: - 2 x BBSRC-IPA grants that received 10% cash contributions from Syngenta Ltd. - A Marie Curie Postdoctoral fellowship awarded to Dr. Allyson MacLean in my group. - Two research projects directly funded by SESVanderHave. - A BBSRC-FAPESP grant in collaboration with colleagues in Brazil. - A BBSRC-LINK grant with SESVanderHave. - iCASE studentships with SESVanderHave and Oxitec. - a Bill and Melinda Gates Foundation grant. - A prestigious interdisciplinary Human Frontier Science Program (HFSP) project with collaborators in the USA, Poland, The Netherlands and the UK. - A BBSRC Future Leader Fellowship won by postdoctoral researcher Dr. Thomas Mathers in my group. - A Newton fellowship awarded to Dr. Adi Kliot in my group. - CEPAMS funds to further develop projects with collaborators at CAS and CAAS in China. - JIC Innovation Fund to further develop technologies for a patent application. - Several DTP studentships. - A PhD studentship funded by the Gatsby Foundation. The above grants have allowed me to generate impact via engaging with industry and (under)graduate students. - My industrial collaborators have used knowledge generated in my group for developing new avenues to control insect pests, including for instance RNAi-mediated strategies and developing markers for breeding crops with increased resistance to pests. My group's interactions with industry have been very productive leading to renewal of contracts and the direct funding of research by industry. - I hosted several undergraduate students in my lab for summer projects and final year research projects. The majority of these students have moved on to pursue a career in science, including obtaining MSc and PhD fellowships. - The majority of PhD students that graduated from my group have careers in science. Several former PhD students are postdoctoral researchers and some lead their own research groups in the UK and other countries. |
| First Year Of Impact | 2014 |
| Sector | Agriculture, Food and Drink,Education,Environment,Government, Democracy and Justice,Culture, Heritage, Museums and Collections,Retail |
| Impact Types | Cultural,Societal,Economic,Policy & public services |
| Description | Project Leader of BRIGIT, a UK-wide consortium to mitigate the risks of Xylella fastidiosa outbreaks in the UK |
| Geographic Reach | Europe |
| Policy Influence Type | Membership of a guideline committee |
| Impact | The BRIGIT consortium includes people from various layers of government, charities, research institutes and industry. The writing of the BRIGIT proposal and activities within BRIGIT so far increased the knowledge of the consortium members about the Xylella pathosystem and how Xylella fastidiosa may spread in the UK and harm the environment. This is likely to influence future regulations to maximize protection of the UK environment. |
| URL | https://www.jic.ac.uk/brigit/ |
| Description | Assess sugarbeet resistance to aphids |
| Amount | £9,870 (GBP) |
| Organisation | Sesvanderhave |
| Sector | Private |
| Country | Belgium |
| Start | 02/2019 |
| End | 02/2020 |
| Description | BBSCR Future Leadership Award awarded to TM |
| Amount | £429,000 (GBP) |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 04/2018 |
| End | 03/2021 |
| Description | BBSRC IPA |
| Amount | £962,887 (GBP) |
| Funding ID | BB/R009481/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 04/2018 |
| End | 03/2021 |
| Description | BBSRC-IPA grant |
| Amount | £1,316,000 (GBP) |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 04/2018 |
| End | 03/2021 |
| Description | Cash contribution to IPA grant |
| Amount | £131,700 (GBP) |
| Organisation | Syngenta International AG |
| Sector | Public |
| Country | Global |
| Start | 04/2018 |
| End | 03/2021 |
| Description | Cash contribution to IPA grant |
| Amount | £95,000 (GBP) |
| Organisation | Syngenta International AG |
| Sector | Public |
| Country | Global |
| Start | 10/2014 |
| End | 09/2016 |
| Description | Future Leader Fellowship awarded to Thomas Mathers |
| Amount | £317,000 (GBP) |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 04/2018 |
| End | 04/2021 |
| Description | Human Frontier Science Program (HFSP) Research Grant |
| Amount | $1,350,000 (USD) |
| Funding ID | RGP0024/2015 |
| Organisation | Human Frontier Science Program (HFSP) |
| Sector | Charity/Non Profit |
| Country | France |
| Start | 08/2015 |
| End | 07/2019 |
| Description | In kind contribution to LINK grant |
| Amount | £414,657 (GBP) |
| Organisation | Sesvanderhave |
| Sector | Private |
| Country | Belgium |
| Start | 04/2016 |
| End | 09/2019 |
| Description | Institute Innovation Funds |
| Amount | £124,000 (GBP) |
| Organisation | John Innes Centre |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 11/2018 |
| End | 12/2020 |
| Description | JIC-CAAS collaboration funds |
| Amount | £100,000 (GBP) |
| Organisation | John Innes Centre |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 04/2017 |
| End | 12/2018 |
| Description | PhD Student Fellowship |
| Amount | £23,977 (GBP) |
| Organisation | Gatsby Charitable Foundation |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 10/2018 |
| End | 09/2022 |
| Description | The Royal Society Kohn Fellowship awarded to Adi Kliot |
| Amount | £65,000 (GBP) |
| Organisation | The Royal Society |
| Sector | Academic/University |
| Country | United Kingdom |
| Start | 10/2016 |
| End | 10/2018 |
| Description | US Partnership Award |
| Amount | £50,000 (GBP) |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 06/2016 |
| End | 06/2018 |
| Description | iCASE PhD studentship |
| Amount | £12,400 (GBP) |
| Organisation | Oxitec Ltd |
| Sector | Private |
| Country | United Kingdom |
| Start | 10/2015 |
| End | 09/2019 |
| Description | iCASE studentship with Oxitec |
| Amount | £20,000 (GBP) |
| Organisation | Oxitec Ltd |
| Sector | Private |
| Country | United Kingdom |
| Start | 10/2015 |
| End | 10/2019 |
| Description | Collaboration with Anna-Maria Botha-Oberholster |
| Organisation | University of Stellenbosch |
| Country | South Africa |
| Sector | Academic/University |
| PI Contribution | We will sequence and annotate the genome of one clone of Diuraphis noxia, a serious pest of wheat in South Africa and many other countries worldwide |
| Collaborator Contribution | Contributed frozen materials for D. noxio aphids |
| Impact | We generated a genome assembly of D. noxia. |
| Start Year | 2016 |
| Description | Collaboration with Dr. Glen Powell |
| Organisation | East Malling Research |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | We are sequencing and annotating the genome of the woolly apple aphid, a serious pest of apple trees |
| Collaborator Contribution | The collaborator provided frozen aphids for genome and RNA sequencing |
| Impact | We will obtain the complete genomes and transcriptomes of the woolly apple aphid, which is in a distinct clade in the aphid phylogenetic tree and useful for comparative genome analyses among aphids. |
| Start Year | 2018 |
| Description | Collaboration with Dr. Sofia Seabra and Dr. Teresa Rebelo |
| Organisation | University of Lisbon |
| Country | Portugal |
| Sector | Academic/University |
| PI Contribution | I involved colleagues at University of Lisbon in the BRIGIT project and sequenced the genomes and transcriptomes of insect vectors of Xylella fastidiosa |
| Collaborator Contribution | Partners collected insects in Portugal and send these to us for sequencing. |
| Impact | Generated new knowledge on population structures of X. fastidiosa insect vectors. |
| Start Year | 2018 |
| Description | Collaboration with Prof. Ryohei Terauchi |
| Organisation | University of Kyoto |
| Country | Japan |
| Sector | Academic/University |
| PI Contribution | Collaborator is involved in a research project of a PhD student in the lab |
| Collaborator Contribution | Collaborator provided transgenic plant lines for screening |
| Impact | Progress made with research goals of PhD student project |
| Start Year | 2018 |
| Description | Collaborations with The Sainsbury Laboratory |
| Organisation | John Innes Centre |
| Department | The Sainsbury Laboratory |
| Country | United Kingdom |
| Sector | Charity/Non Profit |
| PI Contribution | Provide knowledge on the aphid-plant interactions experimental system. |
| Collaborator Contribution | Dr. Frank Menke and Dr. Jan Sklenar have contributed knowledge and expertise to a PhD student project in my lab. |
| Impact | The PhD student made good progress with achieving research goals of his PhD project. |
| Start Year | 2017 |
| Description | Formal collaboration with Dr. Ana Perez-Sierra, Dr. Mariella Marzano and Dr. Sietse van der Linde |
| Organisation | Forest Research |
| Country | United Kingdom |
| Sector | Public |
| PI Contribution | I involved colleagues at Forest Research in the BRIGIT project |
| Collaborator Contribution | Dr. Ana Perez-Sierra leads Work Package 1 (WP1) of the BRIGIT project. The main aims of WP1 are to develop activities to involved citizen scientists and stakeholders in the BRIGIT project and develop databases with information on various aspects of Xylella fastifiosa and its insect vectors. Dr. Sietse van der Linde is involved in WP2 of the BRIGIT project. WP2 is focused on developing diagnostics for X. fastidiosa and is lead by colleagues at Fera. Dr. Mariella Marzano is involved in developing the social sciences aspect of WP4 of the BRIGIT project; WP4 is lead by Dr. Steven White (CEH). |
| Impact | Planning of citizen scientist and stakeholder activities have started. |
| Start Year | 2018 |
| Description | Formal collaboration with Dr. Daniel Chapman |
| Organisation | University of Stirling |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | I involved Dr. Chapman in the BRIGIT project. |
| Collaborator Contribution | Dr. Chapman participates in Work Package 4 (WP4) of the BRIGIT project. The aim of WP4 is to model the potential pathways of spread of Xylella fastidiosa in the UK. WP4 is lead by Steven White (CEH). |
| Impact | Collating data for starting the modelling of WP4 of BRIGIT has started. |
| Start Year | 2018 |
| Description | Formal collaboration with Dr. David Kenyon, Dr. Fiona Highet and Dr. Karen Fraser |
| Organisation | Science and Advice for Scottish Agriculture |
| Country | United Kingdom |
| Sector | Public |
| PI Contribution | I involved colleagues at SASA in the BRIGIT project |
| Collaborator Contribution | Colleagues at SASA are involved in Work Package (WP) 2 and 3 of the BRIGIT project. WP2 is aimed at optimizing and further developing technologies for diagnostics of Xylella fastidiosa within BRIGIT, and WP3 is aimed at better understanding the migration of X. fastidiosa insect vectors in the UK. WP2 is lead by Fera and WP3 by JIC (myself). |
| Impact | Work on WP2 and WP3 have started |
| Start Year | 2018 |
| Description | Formal collaboration with Dr. Gerard Clover |
| Organisation | Royal Horticultural Society |
| Country | United Kingdom |
| Sector | Charity/Non Profit |
| PI Contribution | I involved the Royal Horticultural Society in the BRIGIT project. |
| Collaborator Contribution | Dr. Gerard Clover and colleague Sarah Plummer develop a database of plant species susceptible to Xylella fastidiosa. |
| Impact | Dr. Gerard Clover has been hired as the Engagement Officer for the BRIGIT project and works 4 days per week at the JIC |
| Start Year | 2018 |
| Description | Formal collaboration with Dr. John Elphinstone, Dr. Jenny Tomlinson, Dr. Glyn Jones, Dr. Chris Malumphy and others |
| Organisation | Fera Science Limited |
| Country | United Kingdom |
| Sector | Multiple |
| PI Contribution | I involved colleauges at Fera in the BRIGIT project |
| Collaborator Contribution | Dr. Elphinstone and Dr. Tomlinson lead Work Package 2 (WP2) of the BRIGIT project. The main goal of WP2 is to optimize and improve technologies for diagnostics of Xylella fastidiosa in diverse plant species, including trees. Dr. Chris Malumphy is involved in WP3 of the BRIGIT project. The main goal of WP3 is to better understand the migration patterns of X. fastidiosa insect vectors in the UK. WP3 is lead by Saskia Hogenhout (me). Dr. Glyn Jones in is involved in the social sciences aspect of WP4 of the BRIGIT project. The main goal of WP4 is to model the potential pathways of spread of X. fastidiosa in the UK. WP4 is lead by Steven White (CEH). |
| Impact | Protocol optimization for diagnostics of Xylella fastidiosa has started. |
| Start Year | 2018 |
| Description | Formal collaboration with Dr. Stephen Parnell |
| Organisation | University of Salford |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | I involved Dr. Parnell in the BRIGIT project. |
| Collaborator Contribution | Dr. Parnell participates in Work Package (WP4) of the BRIGIT project. WP4 is modelling the potential pathways of spread of Xylella fastidiosa in the UK. WP4 is lead by Steven White (CEH). |
| Impact | Collation of data for the modelling of WP4 has started. |
| Start Year | 2018 |
| Description | Formal collaboration with Dr. Steven White |
| Organisation | Natural Environment Research Council |
| Department | Centre for Ecology & Hydrology (CEH) |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | I involved Dr. Steven White in the BRIGIt project |
| Collaborator Contribution | 1. Leads Work Package 4 of the BRIGIT project; 2. Models the potential pathways of spread of Xylella fastidiosa in the UK |
| Impact | Started to collate data from project partners to do the modelling and interacts with scientists in Europe to help develop the model. |
| Start Year | 2018 |
| Description | Formal collaboration with Dr. Tracey Chapman |
| Organisation | University of East Anglia |
| Department | School of Biological Sciences UEA |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | I am in the supervisory committee of a PhD student in the Chapman lab (iCASE studentship with Oxitec). |
| Collaborator Contribution | Dr. Chapman is in the supervisory committee of my PhD student (iCASE studentship with Oxitec). |
| Impact | Gained new insights on how to control insect pests of plants. |
| Start Year | 2015 |
| Description | Formal research collaboration with Abdullah Al-Sadi, Sultan Qaboos University, Muscat, Oman. |
| Organisation | Sultan Qaboos University |
| Department | Department of Crop Sciences |
| Country | Oman |
| Sector | Academic/University |
| PI Contribution | Provided intellectual input into investigating an important pathogen of lime trees in Oman, and hosted Ali Al-Subhi, PhD student at Sultan Qaboos University, to conduct molecular research on the pathogen for 6 months in 2015/2016 and 3 months in 2017. |
| Collaborator Contribution | Provided funding for research conducted by the visiting PhD student and hosted me to visit phytoplasma-infected lime orchards and vegetable farms in Oman. |
| Impact | Co-authored scientific publications, including Al-Subhi et al., 2017. BMC Microbiol. 17: 221. |
| Start Year | 2014 |
| Description | Formal research collaboration with Chih-Horng Kuo, Academia Sinica, Taipei, Taiwan |
| Organisation | Academia Sinica |
| Department | Institute of Plant and Microbial Biology |
| Country | Taiwan, Province of China |
| Sector | Academic/University |
| PI Contribution | I provided intellectual input into the development of the research question, approaches and methodology. My team provided knowledge about the biology of the organisms, collected biological material, extracted DNA and generated raw reads of genome sequence data. |
| Collaborator Contribution | The partner assembled the genome reads into contigs and annotated the genomes. |
| Impact | Several co-authored publications, for example: Orlovskis et al., 2017. Ann Bot. 119: 869-884. |
| Start Year | 2014 |
| Description | Formal research collaboration with Cock van Oosterhout, University of East Anglia, Norwich, UK |
| Organisation | University of East Anglia |
| Department | School of Environmental Sciences UEA |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Our time provides knowledge, resources and materials for the insect-plant interaction research system. |
| Collaborator Contribution | The Van Oosterhout team provides knowledge on evolutionary biology and population genetics. |
| Impact | We obtained two BBSRC-IPA collaborative grants with Syngenta. We co-supervise postdoctoral researchers and PhD students. We are co-authors on Mathers et al., 2017. Genome Biology, and a paper that is being submitted this month. We assisted postdoctoral researcher Thomas Mathers with the writing of a Future leader fellowship application, which was succesful (starts Apr 2018). |
| Start Year | 2013 |
| Description | Formal research collaboration with David Swarbreck, Earlham Institute, Norwich, UK |
| Organisation | Earlham Institute |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Our time provides knowledge, resources and materials for the insect-plant interaction research system. |
| Collaborator Contribution | The Swarbreck team provides knowledge on bioinformatics and genomics, including genome and transcriptome assembly pipelines and genome annotations. |
| Impact | We obtained two BBSRC-IPA collaborative grants with Syngenta. We co-supervise postdoctoral researchers and PhD students. We are co-authors on Mathers et al., 2017. Genome Biology, and a paper that is being submitted this month. We assisted postdoctoral researcher Thomas Mathers with the writing of a Future leader fellowship application, which was succesful (starts Apr 2018). |
| Start Year | 2010 |
| Description | Formal research collaboration with Henryk Pospieszny, Institute of Plant Protection, Poznan, Poland |
| Organisation | Institute of Plant Protection, National Research Institute |
| Country | Poland |
| Sector | Public |
| PI Contribution | My lab hosted Agnieszka Zwolinska, a PhD student at the Institute of Plant Protection, Oct 2016 - Mar 2017. Agnieszka conducted work on the HFSP collaborative project. |
| Collaborator Contribution | The team in Poznan collects plant and insect samples in northern and southern regions of Poland for the HFSP project; the samples are assayed for the presence of phytoplasma strains and effector genes. |
| Impact | We wrote a HFSP project proposal together that was funded. We hold regular Skype meetings (in average, once in the 6 weeks) to discuss progress and results. |
| Start Year | 2014 |
| Description | Formal research collaboration with Lan-Qin Xia and Ju-Lian Chen, CAAS, Beijing, China |
| Organisation | Chinese Academy of Agricultural Sciences |
| Country | China |
| Sector | Academic/University |
| PI Contribution | My group provides aphid genomics information to the project. |
| Collaborator Contribution | CAAS contributed aphid samples and transgenic wheat to achieve plant-mediated RNAi of aphids to the project |
| Impact | We sequenced the genomes of many wheat-colonizing aphid species. The genomes have been assembled. Annotation pipeline for the genomes is under construction. |
| Start Year | 2016 |
| Description | Formal research collaboration with Martina Seruga-Music, University of Zagreb, Croatia |
| Organisation | University of Zagreb |
| Department | Department of Molecular Biology |
| Country | Croatia |
| Sector | Academic/University |
| PI Contribution | Provided expertise on genome-wide analyses and identification and functional characterization of effectors of phytoplasmas. Hosted members of the Croatia team several times and helped the team to obtain a research grant in Croatia. |
| Collaborator Contribution | The Croatia team provided useful sequence information to my team and contributed to the writing of publications. |
| Impact | Exchanged useful data and co-published scientific publications. Another publication is in preparation and will be submitted in March 2018. |
| Start Year | 2010 |
| Description | Formal research collaboration with Richard Immink, Wageningen University and Research Centre, The Netherlands |
| Organisation | University of Wageningen |
| Department | Department of Plant Sciences |
| Country | New Zealand |
| Sector | Academic/University |
| PI Contribution | We provided knowledge on phytoplasma virulence proteins that interfere with plant development and involved the Wageningen team into a interdisciplinary project proposal that received funding from HFSP. |
| Collaborator Contribution | The Wageningen team provided expertise on plant transcription factors targeted by phytoplasma effectors and helped us with interpretation of the plant developmental phenotypes. They are co-investigators on our HFSP grant. |
| Impact | We received funding for a HFSP research project. We co-published a paper: MacLean et al., 2014. PLoS Biol. 12(4):e1001835. We co-supervise postdoctoral researchers. We hold regular Skype meetings (in average once per 6 months) for the past 3 years. |
| Start Year | 2014 |
| Description | Formal research collaboration with Russel Groves, University of Wisconsin, Madison, USA |
| Organisation | University of Wisconsin-Madison |
| Department | Department of Entomology |
| Country | United States |
| Sector | Academic/University |
| PI Contribution | We involved the University of Wisconsin team into a HFSP research proposal that got funded. |
| Collaborator Contribution | The University of Wisconsin team collects phytoplasma-infected plant and leafhopper samples from all over the USA for the HFSP-funded project. |
| Impact | We received funding for collaborative HFSP project. We hold regular progress meeting (in average once/6 weeks). We co-supervise postdoctoral researchers and PhD students. We are writing up a manuscript. |
| Start Year | 2014 |
| Description | Formal research collaboration with SESVanderHave |
| Organisation | Sesvanderhave |
| Country | Belgium |
| Sector | Private |
| PI Contribution | My research group has provided advise on strategies to obtain aphid resistant sugar beet, exchanged knowledge on research progress in plant-insect interactions of the lab, and wrote BBRSC-LINK award to fund research. |
| Collaborator Contribution | SESVanderHave provides access to sugar beet breeding lines, genome sequence resources for these lines and insecticide-free field sites for collection of aphid populations in UK and Europe. They also funded a postdoctoral researcher in my group for one year, contributed 50% in-kind funds for the BBSRC-LINK award and funds a iCASE studentship in my group. PhD student Roland Wouters was recruited for the iCASE project. Roland is making good progress. |
| Impact | Generated knowledge on plant-insect interactions. Organized visits of the SESVanderHave team to JIC (two times per year) and my group at JIC to SESVanderHave headquarters in Tienen, Belgium (two times per year). Organized regular Skype calls to discuss research progress and ideas for future research. |
| Start Year | 2013 |
| Description | Formal research collaboration with Syngenta |
| Organisation | Syngenta International AG |
| Department | Syngenta Ltd (Bracknell) |
| Country | United Kingdom |
| Sector | Private |
| PI Contribution | Provide information about aphid biology and genomics, and mechanisms involved in aphid adjustment to diverse plant species and pesticides. |
| Collaborator Contribution | Contributed 10% cash towards two BBSRC-IPA projects (2014-2017; 2017-2020) and advised on how to conduct pesticide applications. Provided aphid clones for sequencing and analyses. |
| Impact | Made progress with understanding processes involved in aphid adjustments to plants and pesticides. This collaboration resulted in a publication: Mathers, Chen et al., 2017. Genome Biol. 18: 27. As well, the collaboration was renewed with follow-up funding, that is a BBSRC-IPA grant commencing April 2018. Held regular meetings to discuss progress, including visits of my group to Syngenta and collagues of Syngenta to JIC, and Skype and phone conference meetings. |
| Start Year | 2014 |
| Description | Formal research collaboration with Yongping Huang, SIPPE, CAS, Shanghai, China |
| Organisation | Chinese Academy of Sciences |
| Department | Shanghai Institute of Plant Physiology and Ecology |
| Country | China |
| Sector | Academic/University |
| PI Contribution | My group provides knowledge, resources and materials, and (genome/gene) sequence information of various species of aphids. |
| Collaborator Contribution | Huang's group provides information on insect transformation methods, insect sex determination genes, baculovirus and insect cell-based expression systems. |
| Impact | We excnahed visits and attended (CEPAMS) meetings to discuss research progress. Qun Liu, Yazhou Chen and Weijie Huang, former postdoctoral researchers and PhD students at SIPPE, are currently postdoctoral researchers in the Hogenhout group. |
| Start Year | 2014 |
| Description | Partnership award with Matthew Baylis, University of Liverpool, UK. |
| Organisation | University of Liverpool |
| Department | School of Veterinary Science Liverpool |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Provide information on vector-borne diseases of plants. |
| Collaborator Contribution | Provide information of vector-borne diseases of humans and animals. |
| Impact | We won a US Partnering Award: Vector-borne diseases in the UK & US: common threats and shared solutions" [Matthew Baylis, PI (PI, University of Liverpool), Saskia Hogenhout (co-PI, JIC) and Simon Carpenter (co-PI, The Pirbright Institute)] BBSRC £50k, and co-organized visits of US colleagues to the UK (Dec 2016) and UK group members to the University of California, Davis (Oct 2017). We applied for a GCRF VBD network grant (£4M) together; the pre-proposal for this was selected for submission of a full proposal, and we were invited for an interview with BBSRC based on our full proposal submission. The proposal was ranked 5th out 12 proposals, and only the top 3 were funded. Finally, we are co-organizing Vector-Borne Diseases in the UK meeting, 3-4 Dec 2018 that will be hosted at the JIC. |
| Start Year | 2016 |
| Description | Partnership with Dr. Nicola Spence and Dr. Mike Sutton-Croft, Defra |
| Organisation | Department For Environment, Food And Rural Affairs (DEFRA) |
| Country | United Kingdom |
| Sector | Public |
| PI Contribution | I engaged with Drs. Spence and Sutton-Croft to develop the BRIGIT proposal. We are currently working on response policies in the event Xylella fastidiosa is detected in the UK and strategies for integration of outcomes of the BRIGIT project in these reponse policies. |
| Collaborator Contribution | Contribution of a wide range of knowledge on policy making and (methods for) collection of data for the BRIGIT project. |
| Impact | We have weekly meetings when Dr. Sutton-Croft is at the John Innes Centre at one day per week. |
| Start Year | 2018 |
| Description | AMM at APS, Minneapolis, USA |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | A postdoc presented an invited talk in teh session Interconnected lifecycles: multitrophic interactions between plants, pathogens and insects at the American Phytopathological Society (APS) - Canadian Phytopathological Society Joint Meeting, Minneapolis, USA, 9-13 August 2014. The talk sparked questions and discussion afterwards. |
| Year(s) Of Engagement Activity | 2014 |
| Description | APS, Tampa, FL, USA |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Presented a research talk about my lab's recent progress made in functional analyses of phytoplasma effector proteins and how they change plant development and plant susceptibility to insect pests at the American Plant Pathology Society meeting, Tampa, Florida. |
| Year(s) Of Engagement Activity | 2016 |
| Description | Annual Symposium Lausanne, Switserland |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Presented a seminar in the Annual Symposium Department of Fundamental Microbiology, University of Lausanne, Lausanne, Switzerland, 7 Nov 2014. This sparked questions and discussion afterwards. I engaged with leading and young scientists to discuss research. |
| Year(s) Of Engagement Activity | 2014 |
| Description | Attended meeting at Bill and Melinda Gates foundation |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Supporters |
| Results and Impact | Attended a meeting and presented a poster at the Grand Challenges; Explorations Agricultural Development (Round 8 & 9) and Nutrition (Round 7 & 8) Convening, Bill and Melinda Gates foundation, Seattle, Washington, USA, May 2013. Engaged with African scientists and assessed if the technology produced in this project is useful for applications to protect crops from whitefly pests and research of colleagues. |
| Year(s) Of Engagement Activity | 2013 |
| Description | BSCD and BSDB |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Presented a research talk about my lab's recent progress made in functional analyses of phytoplasma effector proteins and how they change plant development and plant susceptibility to insect pests. |
| Year(s) Of Engagement Activity | 2016 |
| Description | Biotechnology conference, Cuba |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Presented a talk in a parallel session at the Biotechnology Conference, Havana, Cuba, 1-5 Dec 2014. This sparked questions and discussion afterwards. |
| Year(s) Of Engagement Activity | 2014 |
| Description | COST meeting, Copenhagen, Denmark |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | I have a talk at the Plant-Insect-Microbe Interactions Symposium, Copenhagen, Denmark, 16 April 2015. This sparked questions and discussion afterwards. |
| Year(s) Of Engagement Activity | 2015 |
| Description | Chair of Insectary Platform Steering Committee |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Professional Practitioners |
| Results and Impact | Chair of committee that ensures that the JIC Insectary/Entomology Technology Platform is well managed, proactive, effective, state of the art and resourced to meet the Institutes' science needs. |
| Year(s) Of Engagement Activity | 2017,2018,2019 |
| Description | Chair of Technical Platform Oversight Committee (TPOC) |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Professional Practitioners |
| Results and Impact | Chair the committee that ensures that all JIC Technology Platforms are collectively well managed, proactive, effective, state of the art and resourced to meet the Institutes' science needs. |
| Year(s) Of Engagement Activity | 2017,2018,2019 |
| Description | Chair of the Library and Information Services Steering Group, JIC |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Professional Practitioners |
| Results and Impact | Make decisions on Library and Information Services at JIC. |
| Year(s) Of Engagement Activity | 2013,2014,2015,2016,2017 |
| Description | Chaired session at IPMB, Iguassu Falls, Argentina |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Organized and chaired a parallel session on plant-insect interactions at the International Plant Molecular Biology 2015 meeting, Iguassu Falls, Argentina - Brazil, 25-30 October 2015. Established contacts with leaders in the field and engaged with students and postdoctoral researchers. |
| Year(s) Of Engagement Activity | 2015 |
| Description | Co-chaired session at MPMI meeting, Rhodes, Greece |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Co-chaired concurrent session Neglected Systems at the International Congress of Molecular Plant-Microbe Interactions (MPMI), Rhodes, Greece, 6-10 July 2014. I established contacts with leaders in the MPMI field and engaged with students and postdoctoral scientists. |
| Year(s) Of Engagement Activity | 2014 |
| Description | Co-chaired session at SEB, Manchester, UK |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | I organized a co-chaired the session Mechanisms of Plant-Insect Interactions at the Society of Experimental Biology (SEB) meeting, Manchester, UK, 1-5 July 2014. I invited colleagues who are leaders in the field of Plant-Insect Interactions from academic institutions worldwide to present talks in this session. A student and a postdoc in my team presented talks as well and engaged with the international speakers and the audience. |
| Year(s) Of Engagement Activity | 2014 |
| Description | Communication of research findings via Natural History magazine |
| Form Of Engagement Activity | A magazine, newsletter or online publication |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Media (as a channel to the public) |
| Results and Impact | MacLean et al. (2014). PLoS Biol. 12: e1001835 was featured in Natural History magazine (published in the United States; mission: to promote public understanding and appreciation of nature and science). |
| Year(s) Of Engagement Activity | 2014 |
| Description | Communication of research findings via The Economist |
| Form Of Engagement Activity | A magazine, newsletter or online publication |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Public/other audiences |
| Results and Impact | MacLean et al. (2014). PLoS Biol. 12: e1001835 was featured in the Science and Technology section of the print edition of The Economist: 'Symbiosis and parasitism: The sorceress's apprentice'. (12 April 2014). |
| Year(s) Of Engagement Activity | 2014 |
| Description | Communication of research findings via international newspapers |
| Form Of Engagement Activity | A magazine, newsletter or online publication |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Public/other audiences |
| Results and Impact | MacLean et al. (2014). PLoS Biol. 12: e1001835 was highlighted in on-line national media of China (China today), Japan, Russia, USA, Vietnam, Indonesia, Greece, Italy, Spain, France, Germany, The Netherlands (Scientias; Nieuws.nl; Quest; Acksmedia) and UK (Daily Mail; EDP24). |
| Year(s) Of Engagement Activity | 2014 |
| Description | Communication of research findings via local newspapers |
| Form Of Engagement Activity | A magazine, newsletter or online publication |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Public/other audiences |
| Results and Impact | MacLean et al. (2014). PLoS Biol. 12: e1001835 was featured in printed editions of the Eastern Daily Press (9 April 2014) and Evening News (9 April 2014): 'The zombie invasion really is coming'. These articles were advertised on billboards 'Norwich zombie plants riddle' in front of news agencies in Norwich. |
| Year(s) Of Engagement Activity | 2014 |
| Description | Communication of research findings via radio |
| Form Of Engagement Activity | A broadcast e.g. TV/radio/film/podcast (other than news/press) |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Public/other audiences |
| Results and Impact | We contributed to Radio: NTR de Kennis van nu, radio 5, The Netherlands (Podcast April 30 2014 by Richard Immink); Moment of Science, Indiana Public Media, USA (broadcasted 2nd week of May 2014). |
| Year(s) Of Engagement Activity | 2014 |
| Description | Communication of research findings via various websites and on-line media |
| Form Of Engagement Activity | Engagement focused website, blog or social media channel |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Public/other audiences |
| Results and Impact | MacLean et al. (2014). PLoS Biol. 12: e1001835 was highlighted on various websites of international media: Nature News; Scientific American; New York Times; Hortidaily (online meeting place for the international horticulture industry) with 2-min video of JIC; Students Science (Society of Science); Science 2.0; Science, Space & Robots; I fucking love science; Otters and Science News; Fearnet; Engadget. |
| Year(s) Of Engagement Activity | 2014 |
| Description | Communication of research findings via video and press release |
| Form Of Engagement Activity | A broadcast e.g. TV/radio/film/podcast (other than news/press) |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Media (as a channel to the public) |
| Results and Impact | We produced a 2-minute video at John Innes Centre (Chris Wilson) to communicate research of MacLean et al. (2014). PLoS Biol. 12: e1001835 to a general audience - released 8 April 2014 along with written press releases of John Innes Centre (Zoe Dunford) and Wageningen University and Research Centre (Erik Toussaint). |
| Year(s) Of Engagement Activity | 2014 |
| Description | Contributed an 'Accessible Science Talk' for the Research and Support Staff Voice (RSSV) |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Professional Practitioners |
| Results and Impact | The JIC Research and Support Staff Voice (RSSV) organised a day (9 Oct 2018) of talks as part of the JIC Annual Science Meeting, delivered by top scientists from JIC and TSL. Every talk was accessible to a wide audience including scientists, support and partnership staff and communicates the great work carried out at JIC and TSL, presented in a manner that will be understandable and interesting for all. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Contributed to a press release |
| Form Of Engagement Activity | A press release, press conference or response to a media enquiry/interview |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Media (as a channel to the public) |
| Results and Impact | Contributed to a press release to announce funding of this project from Bill and Melinda Gates Foundation Grand Challenges Explorations Program (2013). |
| Year(s) Of Engagement Activity | 2013 |
| Description | Contribution of two lectures for BIO-3C20 Molecular Plant-Microbe Interactions course |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Undergraduate students |
| Results and Impact | Postdoctoral researcher Adi Kliot in my lab will contribute two lectures in the BIO-3C20 Molecular Plant-Microbe Interactions, Spring Semester, University of East Anglia, March 2019. |
| Year(s) Of Engagement Activity | 2019 |
| Description | EPS, Soest |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Postgraduate students |
| Results and Impact | I presented a talk at the Dutch Graduate School Experimental Plant Sciences (EPS), Soest, The Netherlands (invited by PhD students), 29-30 Jan 2015. The audience was predominantly postgraduate students. |
| Year(s) Of Engagement Activity | 2015 |
| Description | Engaged with journalists from Africa |
| Form Of Engagement Activity | A press release, press conference or response to a media enquiry/interview |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Media (as a channel to the public) |
| Results and Impact | Engagement with the general public: Presented talk for group of African journalists visiting the JIC, Apr. 2014 |
| Year(s) Of Engagement Activity | 2014 |
| Description | Engagement with BBSRC as part of the BRIGIT project |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Policymakers/politicians |
| Results and Impact | Have weekly meetings with policy makers at BBSRC about new policies that may be developed to reduce the risk of Xylella fastidiosa outbreaks in the UK |
| Year(s) Of Engagement Activity | 2018,2019 |
| Description | Engagement with Defra, Forest Research and APHA as part of the BRIGIT project |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Policymakers/politicians |
| Results and Impact | Have weekly meetings with policy makers at Defra about new policies that may be developed to reduce the risk of Xylella fastidiosa outbreaks in the UK |
| Year(s) Of Engagement Activity | 2018,2019 |
| Description | Engagement with Fera as part of the BRIGIT project |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Industry/Business |
| Results and Impact | Have weekly meetings with Fera about status of Xylella fastidiosa diagnostics that may influence new policies for reducing risk of X. fastidiosa outbreaks in the UK |
| Year(s) Of Engagement Activity | 2018,2019 |
| Description | Engagement with Oxitec |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Industry/Business |
| Results and Impact | Regular meetings with Neil Morrison for supervisory meetings and discussing research progress of the iCASE studentship. |
| Year(s) Of Engagement Activity | 2015,2016,2017,2018,2019 |
| Description | Engagement with SV |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | Three-monthly meetings with industrial collaborators |
| Year(s) Of Engagement Activity | 2017,2018,2019 |
| Description | Engagement with Syngenta |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | Regular meetings with colleagues at Syngenta, Jealott's Hill, UK, and Switserland and USA to discuss project proposals and research progress on aphids. |
| Year(s) Of Engagement Activity | 2010,2011,2012,2013,2014 |
| Description | Engagement with the Royal Horticulticultural Society as part of the BRIGIT project |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Third sector organisations |
| Results and Impact | Have weekly meetings with staff member of RHS to develop a stakeholder engagement plan for BRIGIT |
| Year(s) Of Engagement Activity | 2018,2019 |
| Description | GMI, Vienna |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Presented a research talk about my lab's recent progress made in functional analyses of phytoplasma effector proteins and how they change plant development and plant susceptibility to insect pests at the Gregor Mendel Institute, Vienna, Austria. |
| Year(s) Of Engagement Activity | 2016 |
| Description | HFSP meeting, SIngapore |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Policymakers/politicians |
| Results and Impact | Presented a research talk about my lab's recent progress made in functional analyses of phytoplasma effector proteins and how they change plant development and plant susceptibility to insect pests at the Human Frontier Science Program (HFSP) meeting, Singapore. |
| Year(s) Of Engagement Activity | 2016 |
| Description | Hosted Alexander Stacey, BSc student Biological Sciences, University of East Anglia, UK. |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Undergraduate students |
| Results and Impact | Student worked on a research project in the summer of 2018 and his BSc research project from Jan-Mar '19. |
| Year(s) Of Engagement Activity | 2018,2019 |
| Description | Hosted Alexandra Kolodyazhnaya, BSc student Novosibirsk State University, Russia |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Undergraduate students |
| Results and Impact | Student did a research project in my lab as part of the JIC/TSL/EI International Undergraduate Summer School programme at JIC. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Hosted Libby Hanwell, BSc student Biological Sciences, University of East Anglia, UK |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Undergraduate students |
| Results and Impact | Hosted the student for a research project in the summer of 2018 and her BSc research project from Oct-Dec '18 |
| Year(s) Of Engagement Activity | 2018,2019 |
| Description | Hosted Luke Sherwin, MSc student Molecular Medicine at the University of East Anglia, UK. |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Postgraduate students |
| Results and Impact | MSc project for BIO_6019Y course. Luke wrote a 3000-word project and did a research project Mar-Sep 2018 |
| Year(s) Of Engagement Activity | 2018 |
| Description | Hosting of Milind Sood, BSc student, University of Oxford, UK. |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Undergraduate students |
| Results and Impact | Will do a research project in my lab as part of the JIC/TSL/EI International Undergraduate Summer School programme at JIC, Jun-Aug 2019. |
| Year(s) Of Engagement Activity | 2019 |
| Description | ICE, Florida, USA |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Presented an invited talk at the International Conference of Entomology (ICE), Orlando, Florida, USA. |
| Year(s) Of Engagement Activity | 2016 |
| Description | IMPRS, Jena |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Postgraduate students |
| Results and Impact | Presented a keynote lecture at the 16th IMPRS Symposium (organized by PhD students at Max Planck Institute), Donburg Castle, Jena, Germany. |
| Year(s) Of Engagement Activity | 2017 |
| Description | Invited research seminar at 'When Development meets Stress' symposium, CRAG, Barcelona, Spain |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Presented a talk on Phytoplasma at the 'When Development meets Stress' symposium, Center for Research in Agricultural Genomics (CRAG), Barcelona, Spain, 2-4 Sep 2018. |
| Year(s) Of Engagement Activity | 2018 |
| URL | https://www.bdebate.org/en/forum/when-development-meets-stress-understanding-developmental-reprogram... |
| Description | Invited research seminar at CAS institute |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Presented a research seminar at IGDB, Beijing, China on 16 Mar 2018. Research fellow Thomas Mathers in my lab also contributed a talk. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Invited research seminar at iDiv, Leipzig, Germany |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | I was invited by PhD students to give a research presentation at the Integrative Biodiversity Research Institute (iDiv), Halle-Jena-Leipzig, Germany, 18 Apr 2018. I was hosted by Crispus Mbaluto, PhD student at iDiv. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Invited research seminar at the Max Planck Institute, Cologne, Germany |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | I was invited to give a research seminar at the Max Planck Institute for Plant Breeding, Cologne, Germany. Approximately 50 people, including PhD students, attended. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Invited research seminar at the XI European Congress of Entomology, Naples, Italy |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Presented a research seminar on Phytoplasma in a parallel session focused on insect-transmitted plant pathogens and plant-insect interactions. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Invited research seminar at the annual Life Sciences conference, Beijing, China |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Presented a talk in a paralell session focused on insect pests at the Life Sciences Conference, Beijing, China, 28-31 Oct '19. |
| Year(s) Of Engagement Activity | 2018 |
| Description | MCC at SEB, Manchester, UK |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | A visiting postdoc in the lab presented a talk in the parallel session Mechanisms of Plant-Insect Interactions at the Society of Experimental Biology (SEB) meeting, Manchester, UK, 1-5 July 2014 |
| Year(s) Of Engagement Activity | 2014 |
| Description | Marburg, Germany |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Presented an invited talk at the Frontiers in Microbiology Symposium, Max Planck Institute, Marburg, Germany. |
| Year(s) Of Engagement Activity | 2016 |
| Description | Member Scientific Resources Committee |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Professional Practitioners |
| Results and Impact | Member of a committee that decides on purchases of all types of scientific equipment and organization of JIC infrastructure. |
| Year(s) Of Engagement Activity | 2017,2018,2019 |
| Description | Member of Programme Management Group of JIC Next Generation Infrastructure |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Professional Practitioners |
| Results and Impact | Feb 2018 - current Member of Programme Management Group of JIC Next Generation Infrastructure |
| Year(s) Of Engagement Activity | 2018,2019 |
| Description | Member of Tenure-Track faculty mentoring committee |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Professional Practitioners |
| Results and Impact | Provide advise to young faculty |
| Year(s) Of Engagement Activity | 2016,2017,2018,2019 |
| Description | Member of supervisory committee of PhD student at University of Wisconsin, Madison, USA |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Postgraduate students |
| Results and Impact | Aug 2018 - current: Member of supervisory committee of PhD student Shannon Piper, University of Madison, Wisconsin, USA. |
| Year(s) Of Engagement Activity | 2018,2019 |
| Description | Member of the JIC Distribution of Exploitation Income (DEI) Panel |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Professional Practitioners |
| Results and Impact | Helped to decide on the Distribution of Exploitation Income of colleages at the Norwich Research Park |
| Year(s) Of Engagement Activity | 2012,2013,2014,2015,2016 |
| Description | OARDC, Wooster, OH, USA |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | I presented a talk at my former workplace: The Ohio State University - Ohio Agriculture and Development Center (OARDC), Wooster, Ohio, USA. 10-13 May 2015. This sparked questions and discussions afterwards. |
| Year(s) Of Engagement Activity | 2015 |
| Description | Organizing the satellite meeting 'Dynamics and Mechanism of Insect-Transmitted Pathogens' at the IS-MPMI |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | This satellite meeting will be organized on Sunday 14th July, 8.30 - 15.00, and brings together scientists with an interest in studying the diverse aspects of insect-transmitted plant pathogen interactions with their plant hosts and insect vectors. These pathogens cause the most destructive diseases of plants and are often responsible for eradication of entire crop industries in countries or regions. Current examples include Olive Quick Decline Syndrome (Xylella fastidiosa), citrus greening (Candidatus Liberibacter sp.), Lime Witch's Broom and Grapevine Flavescence doree (Candidatus Phytoplasma) and Cassava mosaic (geminivirus). The meeting will cover (1) Recognition and induction of immunity, (2) Effectors and modulation of insect/plant processes, (3) Epidemiology and insect transmission and (4) Genomics and Evolution. If you would like to attend or contribute to the meeting, please contact Saskia Hogenhout, Silke Robatzek, Sheng-Yang He and Steven Lindow by 15 April 2019 at the very latest. |
| Year(s) Of Engagement Activity | 2018,2019 |
| URL | https://www.ismpmi.org/Congress/2019/program/Pages/Satellite-Meetings.aspx |
| Description | Phytopathological Society, Colmar, France |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | I presented a talk at the French Phytopathology Meeting, Colmar, France, 2-5 June 2015. This sparked questions and discussion afterwards. |
| Year(s) Of Engagement Activity | 2015 |
| Description | Phytopathology conference, Brazil |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | SH presented a talk at the 46th Meeting of the Brazilian Phytopathological Society/11th Brazilian Meeting on Biological Control, Ouro Preto, Brazil, 20-25 October 2013. |
| Year(s) Of Engagement Activity | 2013 |
| Description | Presented a talk at Monogram |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Presented a talk at Monogram, JIC, Norwich, UK, 24-16 Apr 2018. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Presented a talk at a whitefly conference |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Presented a talk (invited based on abstract submission) at the 1st International Whitefly Symposium, Kolymbari, Sicily, Italy, May 2013. |
| Year(s) Of Engagement Activity | 2013 |
| Description | Press release to announce BRIGIT project |
| Form Of Engagement Activity | A press release, press conference or response to a media enquiry/interview |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Media (as a channel to the public) |
| Results and Impact | Engaged with JIC Public Engagement Officer to launch press release 'UK consortium to combat serious threat to plant health' to announce the start of the BRIGIT project and the BRIGIT project website |
| Year(s) Of Engagement Activity | 2018 |
| URL | https://www.jic.ac.uk/press-release/uk-wide-consortium-to-combat-serious-threat-to-plant-health/ |
| Description | Press release to announce new appointment for the BRIGIT project |
| Form Of Engagement Activity | A press release, press conference or response to a media enquiry/interview |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Media (as a channel to the public) |
| Results and Impact | Engaged with JIC Public Engagement Officer to launch press release 'New appointment for UK-wide Xylella pathogen consortium' for the BRIGIT project |
| Year(s) Of Engagement Activity | 2019 |
| URL | https://www.jic.ac.uk/news/new-appointment-for-uk-wide-xylella-pathogen-consortium/ |
| Description | UK Microbiology Society |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Presented a research talk about my lab's recent progress made in functional analyses of phytoplasma effector proteins and how they change plant development and plant susceptibility to insect pests. |
| Year(s) Of Engagement Activity | 2016 |