Functional characterization of phytoplasma effector proteins and the role of salivary gland proteins in plant colonization of aphids

Lead Research Organisation: John Innes Centre
Department Name: Contracts Office

Abstract

The goal of this project is to identify and characterize plant pathogen and pest virulence (effector) proteins that manipulate plants to become better hosts for these pathogens and pests. We have established genome sequence annotation pipelines to identify candidate effector proteins from phytoplasmas (bacterial plant pathogen transmitted by phloem-feeding leafhoppers) and aphids (phloem-feeding insects that transmit a variety of plant diseases).

Some phytoplasmas, including Aster Yellows strain Witches’ Broom (AY-WB), have broad plant host ranges. We have identified several effector proteins that target plant cell nuclei and change plant morphology comparable to that of AY-WB-infected plants. These morphological changes are beneficial for the leafhopper vector, which is required for dissemination of phytoplasmas in nature. The effector protein genes are encoded on potential mobile units (PMUs) that are abundant in the genomes of phytoplasmas and appear to have had major influence on phytoplasma evolution and diversification. Some PMUs are predominantly expressed during AY-WB infection of plants and others in insects. This project will lead to the functional characterization of at least one AY-WB effector.

The majority of plants are resistant against the majority of aphid species, and only some aphid species, such as the generalist Myzus persicae, can feed from multiple plant host species. Insects release a variety of proteins into the plant phloem in their saliva. These proteins can benefit the insect by reprogramming plant development, but may also play a role in plant resistance to insect attack. This project will focus on aphid interactions with Arabidopsis and crop plants. The project will use the expanding genomics resources for Myzus spp. and Arabidopsis and the JIC infrastructure resources to better understand aphid-plant interactions. The aphid salivary proteome will be investigated and the impact of some secreted proteins on plant responses determined.

Publications

10 25 50
 
Title Video Abstract for Genome Biology publication 
Description Genome Biology created a video abstract to explain the science described in the Genome Biology to students. The video shows animation of an aphid changing plant hosts with a narrator who explains what happens in the aphid upon the host change. The video was posted on YouTube. 
Type Of Art Film/Video/Animation 
Year Produced 2017 
Impact So far the video had 488 views on YouTube, and 9 viewers liked the video and none disliked the video. The video has likely increased the amount of attention the Genome Biology article received by different media (current Altmetric attention score of the paper is 110). 
URL https://www.youtube.com/watch?v=EKy_SuaFKMk
 
Title Video showing how aphids sense aphid attack 
Description We launched a short video showing the method by which we can monitor the calcium elevations that occur in a leaf during aphid probing. 
Type Of Art Film/Video/Animation 
Year Produced 2018 
Impact The video will be understood by a wide audience. 
URL https://www.youtube.com/watch?v=CK2iIPfcnvo
 
Description The funding in this project has been used to generate preliminary data for obtaining additional funding. It enabled us to continue projects between funding cycles and start new ones. We generated these data for two systems. One system is focused on the obligate bacterial pathogen Phytoplasma, which is transmitted by sap-feeding insects. The other system is focused on aphids, which are sap-feeding insects that transmit a diverse range of economically important plant pathogens, such as viruses.

For aphids, we generated genome, transcriptome and proteome data that led to the discovery of effector proteins that aphids use to modulate plant processes and successfully colonize plants. We found that the effector proteins are present in the saliva of aphids and that these proteins are inoculated into plants. One effector was located in the cytoplasm of plant cells (Mugford et al., 2017. MPMI). We found that this effector suppresses plant defense responses (Bos et al., 2010. PLoS Genetics; Drurey et al., 2018. Biorxiv). We generated evidence that this effector is conserved among sap-feeding insects (Drurey et al., 2018. Biorxiv) and elucidated candidate plant targets. These findings were communicated to colleagues of the sugarbeet breeding company SESVanderHave who directly funded or co-funded (via a BBSCR-LINK) further research on aphid-plant interactions that includes functional analyses of other aphid effectors and their targets.

We used funds in this project to develop the RNA interference (RNAi) technology to knock down gene expression in aphids. We showed that we can knock down gene expression in aphids via plant-mediated RNAi (Pitino et al., 2013. PLoS One; Coleman et al., 2015. JXB). We also generated a first genome assembly and annotation of the green peach aphid Myzus persicae in collaboration with David Swarbreck at TGAC (now the Earlham Institute). These data led to collaborations with Syngenta Ltd via a BBSRC-IPA grant that is focused on using aphid genome sequence information and RNAi for studying pesticide resistance and plant host adjustment mechanisms in aphids. This collaboration with Syngenta has progressed very well and led to a second BBSRC-IPA grant that started last year. Syngenta is using our findings to inform their strategies to develop RNAi-mediated pest control technologies. The research on RNAi also provided preliminary data to further develop the RNAi technology for whitefly that was funded by the Bill and Melinda Gates Foundation.

We also used funding from this research project to investigate how plants respond to aphid feeding. We found that plants have specific receptors that detect aphid elicitors leading to a specific plant defense response in the plant (Prince et al., 2014. Plant Physiol.). We identified an aphid effector that suppresses this plant defense response (Mugford et al., 2017. MPMI; Drurey et al., 2018. Biorxiv). This work instigated collaborations with colleagues at the John Innes Centre and the University of Madison, Wisconsin, USA, and the funding of PhD studentship to further investigate the nature of the plant defense response. We developed methods to detect local calcium bursts that surround the aphid feeding site and identified plant factors that regulate these calcium elevations (Vincent et al., 2017. Plant Cell). The work resulted in a successful PhD student thesis and defense and is taken further by two PhD students in the lab. One of these students won a prestigious Gatsby fellowship to fund his PhD. The other PhD student has initiated collaborations with colleagues at The Sainsbury Laboratory and at Kyoto University, Japan. Therefore, this project has led to collaborations with colleagues within the JIC and at other research centers in Norwich, USA and in Japan.

The funds also contributed to research that identified and characterized effectors from Phytoplasma. Phytoplasma colonize their host plants and insect vectors. We found that 65% of the Phytoplasma effector genes are upregulated when these bacteria colonize the host plant, whereas 35% of the effector genes are upregulated in the insect vector (MacLean et al., 2011. Plant Physiol). This project contributed to research that identified plant targets for some of Phytoplasma effectors that are upregulated in the plant (Sugio et al., 2011. PNAS; Sugio et al., 2014. New Phytol; MacLean et al., PLoS Biol) and to the demonstration that the effectors suppress plant defense responses to the insect vectors (Sugio et al., 2011. PNAS; MacLean et al., 204; Orlovskis et al., 2016. Front. Biotic Interact.). Based on these data we were successfull at winning a BBSRC-FAPESP grant and the prestigous Human Frontier Science Program (HFSP) research grant, and a Marie Curie fellowship (to Allyson MacLean) and a Newton fellowship (to Adi Kliot). The BBSRC-FAPESP grant was a collaboration with colleagues in Brazil on the maize-pathogenic Maize bushy stunt phytoplasma. A PhD student in my lab visited my collaborator in Brazil and we published part of this research (Orlovkis et al., 2017. Annals Botany). The HFSP grant is for an interdisciplinary project with collaborators in the USA, Poland, The Netherlands and the UK, and involves mechanistic modelling, molecular and biochemistry research, insect assays and field-based work. The writing of several papers to report the outputs of this research is ongoing.
Exploitation Route Our findings are taken forward by academic research laboratories worldwide, including collaborators mentioned above. Our industrial partners are using our data to improve pest control strategies and develop crops that are more resistant to insects. Undergraduate, MSc and PhD students have successfully graduated and are pursuing careers, mostly in science, in the UK and wordwide. Several former postdoctoral researchers are leading their own research laboratories in the UK, France and Canada.
Sectors Agriculture, Food and Drink,Chemicals,Education,Environment

 
Description The research findings discovered in this project provided preliminary data used for obtaining research funding from various sources, including: - 2 x BBSRC-IPA grants that received 10% cash contributions from Syngenta Ltd. - A Marie Curie Postdoctoral fellowship awarded to Dr. Allyson MacLean in my group. - Two research projects directly funded by SESVanderHave. - A BBSRC-FAPESP grant in collaboration with colleagues in Brazil. - A BBSRC-LINK grant with SESVanderHave. - iCASE studentships with SESVanderHave and Oxitec. - a Bill and Melinda Gates Foundation grant. - A prestigious interdisciplinary Human Frontier Science Program (HFSP) project with collaborators in the USA, Poland, The Netherlands and the UK. - A BBSRC Future Leader Fellowship won by postdoctoral researcher Dr. Thomas Mathers in my group. - A Newton fellowship awarded to Dr. Adi Kliot in my group. - CEPAMS funds to further develop projects with collaborators at CAS and CAAS in China. - JIC Innovation Fund to further develop technologies for a patent application. - Several DTP studentships. - A PhD studentship funded by the Gatsby Foundation. The above grants have allowed me to generate impact via engaging with industry and (under)graduate students. - My industrial collaborators have used knowledge generated in my group for developing new avenues to control insect pests, including for instance RNAi-mediated strategies and developing markers for breeding crops with increased resistance to pests. My group's interactions with industry have been very productive leading to renewal of contracts and the direct funding of research by industry. - I hosted several undergraduate students in my lab for summer projects and final year research projects. The majority of these students have moved on to pursue a career in science, including obtaining MSc and PhD fellowships. - The majority of PhD students that graduated from my group have careers in science. Several former PhD students are postdoctoral researchers and some lead their own research groups in the UK and other countries.
First Year Of Impact 2014
Sector Agriculture, Food and Drink,Education,Environment,Government, Democracy and Justice,Culture, Heritage, Museums and Collections,Retail
Impact Types Cultural,Societal,Economic,Policy & public services

 
Description Project Leader of BRIGIT, a UK-wide consortium to mitigate the risks of Xylella fastidiosa outbreaks in the UK
Geographic Reach Europe 
Policy Influence Type Membership of a guideline committee
Impact The BRIGIT consortium includes people from various layers of government, charities, research institutes and industry. The writing of the BRIGIT proposal and activities within BRIGIT so far increased the knowledge of the consortium members about the Xylella pathosystem and how Xylella fastidiosa may spread in the UK and harm the environment. This is likely to influence future regulations to maximize protection of the UK environment.
URL https://www.jic.ac.uk/brigit/
 
Description Assess sugarbeet resistance to aphids
Amount £9,870 (GBP)
Organisation Sesvanderhave 
Sector Private
Country Belgium
Start 02/2019 
End 02/2020
 
Description BBSCR Future Leadership Award awarded to TM
Amount £429,000 (GBP)
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 04/2018 
End 03/2021
 
Description BBSRC IPA
Amount £962,887 (GBP)
Funding ID BB/R009481/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 04/2018 
End 03/2021
 
Description BBSRC-IPA grant
Amount £1,316,000 (GBP)
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 04/2018 
End 03/2021
 
Description Cash contribution to IPA grant
Amount £95,000 (GBP)
Organisation Syngenta International AG 
Sector Public
Country Global
Start 10/2014 
End 09/2016
 
Description Cash contribution to IPA grant
Amount £131,700 (GBP)
Organisation Syngenta International AG 
Sector Public
Country Global
Start 04/2018 
End 03/2021
 
Description Future Leader Fellowship awarded to Thomas Mathers
Amount £317,000 (GBP)
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 04/2018 
End 04/2021
 
Description Human Frontier Science Program (HFSP) Research Grant
Amount $1,350,000 (USD)
Funding ID RGP0024/2015 
Organisation Human Frontier Science Program (HFSP) 
Sector Charity/Non Profit
Country France
Start 08/2015 
End 07/2019
 
Description In kind contribution to LINK grant
Amount £414,657 (GBP)
Organisation Sesvanderhave 
Sector Private
Country Belgium
Start 04/2016 
End 09/2019
 
Description Institute Innovation Funds
Amount £124,000 (GBP)
Organisation John Innes Centre 
Sector Charity/Non Profit
Country United Kingdom
Start 11/2018 
End 12/2020
 
Description JIC-CAAS collaboration funds
Amount £100,000 (GBP)
Organisation John Innes Centre 
Sector Charity/Non Profit
Country United Kingdom
Start 04/2017 
End 12/2018
 
Description PhD Student Fellowship
Amount £23,977 (GBP)
Organisation Gatsby Charitable Foundation 
Sector Charity/Non Profit
Country United Kingdom
Start 10/2018 
End 09/2022
 
Description The Royal Society Kohn Fellowship awarded to Adi Kliot
Amount £65,000 (GBP)
Organisation The Royal Society 
Sector Academic/University
Country United Kingdom
Start 10/2016 
End 10/2018
 
Description US Partnership Award
Amount £50,000 (GBP)
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 06/2016 
End 06/2018
 
Description iCASE PhD studentship
Amount £12,400 (GBP)
Organisation Oxitec Ltd 
Sector Private
Country United Kingdom
Start 10/2015 
End 09/2019
 
Description iCASE studentship with Oxitec
Amount £20,000 (GBP)
Organisation Oxitec Ltd 
Sector Private
Country United Kingdom
Start 10/2015 
End 10/2019
 
Description Collaboration with Anna-Maria Botha-Oberholster 
Organisation University of Stellenbosch
Country South Africa 
Sector Academic/University 
PI Contribution We will sequence and annotate the genome of one clone of Diuraphis noxia, a serious pest of wheat in South Africa and many other countries worldwide
Collaborator Contribution Contributed frozen materials for D. noxio aphids
Impact We generated a genome assembly of D. noxia.
Start Year 2016
 
Description Collaboration with Dr. Glen Powell 
Organisation East Malling Research
Country United Kingdom 
Sector Academic/University 
PI Contribution We are sequencing and annotating the genome of the woolly apple aphid, a serious pest of apple trees
Collaborator Contribution The collaborator provided frozen aphids for genome and RNA sequencing
Impact We will obtain the complete genomes and transcriptomes of the woolly apple aphid, which is in a distinct clade in the aphid phylogenetic tree and useful for comparative genome analyses among aphids.
Start Year 2018
 
Description Collaboration with Dr. Sofia Seabra and Dr. Teresa Rebelo 
Organisation University of Lisbon
Country Portugal 
Sector Academic/University 
PI Contribution I involved colleagues at University of Lisbon in the BRIGIT project and sequenced the genomes and transcriptomes of insect vectors of Xylella fastidiosa
Collaborator Contribution Partners collected insects in Portugal and send these to us for sequencing.
Impact Generated new knowledge on population structures of X. fastidiosa insect vectors.
Start Year 2018
 
Description Collaboration with Prof. Ryohei Terauchi 
Organisation University of Kyoto
Country Japan 
Sector Academic/University 
PI Contribution Collaborator is involved in a research project of a PhD student in the lab
Collaborator Contribution Collaborator provided transgenic plant lines for screening
Impact Progress made with research goals of PhD student project
Start Year 2018
 
Description Collaborations with The Sainsbury Laboratory 
Organisation John Innes Centre
Department The Sainsbury Laboratory
Country United Kingdom 
Sector Charity/Non Profit 
PI Contribution Provide knowledge on the aphid-plant interactions experimental system.
Collaborator Contribution Dr. Frank Menke and Dr. Jan Sklenar have contributed knowledge and expertise to a PhD student project in my lab.
Impact The PhD student made good progress with achieving research goals of his PhD project.
Start Year 2017
 
Description Formal collaboration with Dr. Ana Perez-Sierra, Dr. Mariella Marzano and Dr. Sietse van der Linde 
Organisation Forest Research
Country United Kingdom 
Sector Public 
PI Contribution I involved colleagues at Forest Research in the BRIGIT project
Collaborator Contribution Dr. Ana Perez-Sierra leads Work Package 1 (WP1) of the BRIGIT project. The main aims of WP1 are to develop activities to involved citizen scientists and stakeholders in the BRIGIT project and develop databases with information on various aspects of Xylella fastifiosa and its insect vectors. Dr. Sietse van der Linde is involved in WP2 of the BRIGIT project. WP2 is focused on developing diagnostics for X. fastidiosa and is lead by colleagues at Fera. Dr. Mariella Marzano is involved in developing the social sciences aspect of WP4 of the BRIGIT project; WP4 is lead by Dr. Steven White (CEH).
Impact Planning of citizen scientist and stakeholder activities have started.
Start Year 2018
 
Description Formal collaboration with Dr. Daniel Chapman 
Organisation University of Stirling
Country United Kingdom 
Sector Academic/University 
PI Contribution I involved Dr. Chapman in the BRIGIT project.
Collaborator Contribution Dr. Chapman participates in Work Package 4 (WP4) of the BRIGIT project. The aim of WP4 is to model the potential pathways of spread of Xylella fastidiosa in the UK. WP4 is lead by Steven White (CEH).
Impact Collating data for starting the modelling of WP4 of BRIGIT has started.
Start Year 2018
 
Description Formal collaboration with Dr. David Kenyon, Dr. Fiona Highet and Dr. Karen Fraser 
Organisation Science and Advice for Scottish Agriculture
Country United Kingdom 
Sector Public 
PI Contribution I involved colleagues at SASA in the BRIGIT project
Collaborator Contribution Colleagues at SASA are involved in Work Package (WP) 2 and 3 of the BRIGIT project. WP2 is aimed at optimizing and further developing technologies for diagnostics of Xylella fastidiosa within BRIGIT, and WP3 is aimed at better understanding the migration of X. fastidiosa insect vectors in the UK. WP2 is lead by Fera and WP3 by JIC (myself).
Impact Work on WP2 and WP3 have started
Start Year 2018
 
Description Formal collaboration with Dr. Gerard Clover 
Organisation Royal Horticultural Society
Country United Kingdom 
Sector Charity/Non Profit 
PI Contribution I involved the Royal Horticultural Society in the BRIGIT project.
Collaborator Contribution Dr. Gerard Clover and colleague Sarah Plummer develop a database of plant species susceptible to Xylella fastidiosa.
Impact Dr. Gerard Clover has been hired as the Engagement Officer for the BRIGIT project and works 4 days per week at the JIC
Start Year 2018
 
Description Formal collaboration with Dr. John Elphinstone, Dr. Jenny Tomlinson, Dr. Glyn Jones, Dr. Chris Malumphy and others 
Organisation Fera Science Limited
Country United Kingdom 
Sector Multiple 
PI Contribution I involved colleauges at Fera in the BRIGIT project
Collaborator Contribution Dr. Elphinstone and Dr. Tomlinson lead Work Package 2 (WP2) of the BRIGIT project. The main goal of WP2 is to optimize and improve technologies for diagnostics of Xylella fastidiosa in diverse plant species, including trees. Dr. Chris Malumphy is involved in WP3 of the BRIGIT project. The main goal of WP3 is to better understand the migration patterns of X. fastidiosa insect vectors in the UK. WP3 is lead by Saskia Hogenhout (me). Dr. Glyn Jones in is involved in the social sciences aspect of WP4 of the BRIGIT project. The main goal of WP4 is to model the potential pathways of spread of X. fastidiosa in the UK. WP4 is lead by Steven White (CEH).
Impact Protocol optimization for diagnostics of Xylella fastidiosa has started.
Start Year 2018
 
Description Formal collaboration with Dr. Stephen Parnell 
Organisation University of Salford
Country United Kingdom 
Sector Academic/University 
PI Contribution I involved Dr. Parnell in the BRIGIT project.
Collaborator Contribution Dr. Parnell participates in Work Package (WP4) of the BRIGIT project. WP4 is modelling the potential pathways of spread of Xylella fastidiosa in the UK. WP4 is lead by Steven White (CEH).
Impact Collation of data for the modelling of WP4 has started.
Start Year 2018
 
Description Formal collaboration with Dr. Steven White 
Organisation Natural Environment Research Council
Department Centre for Ecology & Hydrology (CEH)
Country United Kingdom 
Sector Academic/University 
PI Contribution I involved Dr. Steven White in the BRIGIt project
Collaborator Contribution 1. Leads Work Package 4 of the BRIGIT project; 2. Models the potential pathways of spread of Xylella fastidiosa in the UK
Impact Started to collate data from project partners to do the modelling and interacts with scientists in Europe to help develop the model.
Start Year 2018
 
Description Formal collaboration with Dr. Tracey Chapman 
Organisation University of East Anglia
Department School of Biological Sciences UEA
Country United Kingdom 
Sector Academic/University 
PI Contribution I am in the supervisory committee of a PhD student in the Chapman lab (iCASE studentship with Oxitec).
Collaborator Contribution Dr. Chapman is in the supervisory committee of my PhD student (iCASE studentship with Oxitec).
Impact Gained new insights on how to control insect pests of plants.
Start Year 2015
 
Description Formal research collaboration with Abdullah Al-Sadi, Sultan Qaboos University, Muscat, Oman. 
Organisation Sultan Qaboos University
Department Department of Crop Sciences
Country Oman 
Sector Academic/University 
PI Contribution Provided intellectual input into investigating an important pathogen of lime trees in Oman, and hosted Ali Al-Subhi, PhD student at Sultan Qaboos University, to conduct molecular research on the pathogen for 6 months in 2015/2016 and 3 months in 2017.
Collaborator Contribution Provided funding for research conducted by the visiting PhD student and hosted me to visit phytoplasma-infected lime orchards and vegetable farms in Oman.
Impact Co-authored scientific publications, including Al-Subhi et al., 2017. BMC Microbiol. 17: 221.
Start Year 2014
 
Description Formal research collaboration with Chih-Horng Kuo, Academia Sinica, Taipei, Taiwan 
Organisation Academia Sinica
Department Institute of Plant and Microbial Biology
Country Taiwan, Province of China 
Sector Academic/University 
PI Contribution I provided intellectual input into the development of the research question, approaches and methodology. My team provided knowledge about the biology of the organisms, collected biological material, extracted DNA and generated raw reads of genome sequence data.
Collaborator Contribution The partner assembled the genome reads into contigs and annotated the genomes.
Impact Several co-authored publications, for example: Orlovskis et al., 2017. Ann Bot. 119: 869-884.
Start Year 2014
 
Description Formal research collaboration with Cock van Oosterhout, University of East Anglia, Norwich, UK 
Organisation University of East Anglia
Department School of Environmental Sciences UEA
Country United Kingdom 
Sector Academic/University 
PI Contribution Our time provides knowledge, resources and materials for the insect-plant interaction research system.
Collaborator Contribution The Van Oosterhout team provides knowledge on evolutionary biology and population genetics.
Impact We obtained two BBSRC-IPA collaborative grants with Syngenta. We co-supervise postdoctoral researchers and PhD students. We are co-authors on Mathers et al., 2017. Genome Biology, and a paper that is being submitted this month. We assisted postdoctoral researcher Thomas Mathers with the writing of a Future leader fellowship application, which was succesful (starts Apr 2018).
Start Year 2013
 
Description Formal research collaboration with David Swarbreck, Earlham Institute, Norwich, UK 
Organisation Earlham Institute
Country United Kingdom 
Sector Academic/University 
PI Contribution Our time provides knowledge, resources and materials for the insect-plant interaction research system.
Collaborator Contribution The Swarbreck team provides knowledge on bioinformatics and genomics, including genome and transcriptome assembly pipelines and genome annotations.
Impact We obtained two BBSRC-IPA collaborative grants with Syngenta. We co-supervise postdoctoral researchers and PhD students. We are co-authors on Mathers et al., 2017. Genome Biology, and a paper that is being submitted this month. We assisted postdoctoral researcher Thomas Mathers with the writing of a Future leader fellowship application, which was succesful (starts Apr 2018).
Start Year 2010
 
Description Formal research collaboration with Henryk Pospieszny, Institute of Plant Protection, Poznan, Poland 
Organisation Institute of Plant Protection, National Research Institute
Country Poland 
Sector Public 
PI Contribution My lab hosted Agnieszka Zwolinska, a PhD student at the Institute of Plant Protection, Oct 2016 - Mar 2017. Agnieszka conducted work on the HFSP collaborative project.
Collaborator Contribution The team in Poznan collects plant and insect samples in northern and southern regions of Poland for the HFSP project; the samples are assayed for the presence of phytoplasma strains and effector genes.
Impact We wrote a HFSP project proposal together that was funded. We hold regular Skype meetings (in average, once in the 6 weeks) to discuss progress and results.
Start Year 2014
 
Description Formal research collaboration with Lan-Qin Xia and Ju-Lian Chen, CAAS, Beijing, China 
Organisation Chinese Academy of Agricultural Sciences
Country China 
Sector Academic/University 
PI Contribution My group provides aphid genomics information to the project.
Collaborator Contribution CAAS contributed aphid samples and transgenic wheat to achieve plant-mediated RNAi of aphids to the project
Impact We sequenced the genomes of many wheat-colonizing aphid species. The genomes have been assembled. Annotation pipeline for the genomes is under construction.
Start Year 2016
 
Description Formal research collaboration with Martina Seruga-Music, University of Zagreb, Croatia 
Organisation University of Zagreb
Department Department of Molecular Biology
Country Croatia 
Sector Academic/University 
PI Contribution Provided expertise on genome-wide analyses and identification and functional characterization of effectors of phytoplasmas. Hosted members of the Croatia team several times and helped the team to obtain a research grant in Croatia.
Collaborator Contribution The Croatia team provided useful sequence information to my team and contributed to the writing of publications.
Impact Exchanged useful data and co-published scientific publications. Another publication is in preparation and will be submitted in March 2018.
Start Year 2010
 
Description Formal research collaboration with Richard Immink, Wageningen University and Research Centre, The Netherlands 
Organisation University of Wageningen
Department Department of Plant Sciences
Country New Zealand 
Sector Academic/University 
PI Contribution We provided knowledge on phytoplasma virulence proteins that interfere with plant development and involved the Wageningen team into a interdisciplinary project proposal that received funding from HFSP.
Collaborator Contribution The Wageningen team provided expertise on plant transcription factors targeted by phytoplasma effectors and helped us with interpretation of the plant developmental phenotypes. They are co-investigators on our HFSP grant.
Impact We received funding for a HFSP research project. We co-published a paper: MacLean et al., 2014. PLoS Biol. 12(4):e1001835. We co-supervise postdoctoral researchers. We hold regular Skype meetings (in average once per 6 months) for the past 3 years.
Start Year 2014
 
Description Formal research collaboration with Russel Groves, University of Wisconsin, Madison, USA 
Organisation University of Wisconsin-Madison
Department Department of Entomology
Country United States 
Sector Academic/University 
PI Contribution We involved the University of Wisconsin team into a HFSP research proposal that got funded.
Collaborator Contribution The University of Wisconsin team collects phytoplasma-infected plant and leafhopper samples from all over the USA for the HFSP-funded project.
Impact We received funding for collaborative HFSP project. We hold regular progress meeting (in average once/6 weeks). We co-supervise postdoctoral researchers and PhD students. We are writing up a manuscript.
Start Year 2014
 
Description Formal research collaboration with SESVanderHave 
Organisation Sesvanderhave
Country Belgium 
Sector Private 
PI Contribution My research group has provided advise on strategies to obtain aphid resistant sugar beet, exchanged knowledge on research progress in plant-insect interactions of the lab, and wrote BBRSC-LINK award to fund research.
Collaborator Contribution SESVanderHave provides access to sugar beet breeding lines, genome sequence resources for these lines and insecticide-free field sites for collection of aphid populations in UK and Europe. They also funded a postdoctoral researcher in my group for one year, contributed 50% in-kind funds for the BBSRC-LINK award and funds a iCASE studentship in my group. PhD student Roland Wouters was recruited for the iCASE project. Roland is making good progress.
Impact Generated knowledge on plant-insect interactions. Organized visits of the SESVanderHave team to JIC (two times per year) and my group at JIC to SESVanderHave headquarters in Tienen, Belgium (two times per year). Organized regular Skype calls to discuss research progress and ideas for future research.
Start Year 2013
 
Description Formal research collaboration with Syngenta 
Organisation Syngenta International AG
Department Syngenta Ltd (Bracknell)
Country United Kingdom 
Sector Private 
PI Contribution Provide information about aphid biology and genomics, and mechanisms involved in aphid adjustment to diverse plant species and pesticides.
Collaborator Contribution Contributed 10% cash towards two BBSRC-IPA projects (2014-2017; 2017-2020) and advised on how to conduct pesticide applications. Provided aphid clones for sequencing and analyses.
Impact Made progress with understanding processes involved in aphid adjustments to plants and pesticides. This collaboration resulted in a publication: Mathers, Chen et al., 2017. Genome Biol. 18: 27. As well, the collaboration was renewed with follow-up funding, that is a BBSRC-IPA grant commencing April 2018. Held regular meetings to discuss progress, including visits of my group to Syngenta and collagues of Syngenta to JIC, and Skype and phone conference meetings.
Start Year 2014
 
Description Formal research collaboration with Yongping Huang, SIPPE, CAS, Shanghai, China 
Organisation Chinese Academy of Sciences
Department Shanghai Institute of Plant Physiology and Ecology
Country China 
Sector Academic/University 
PI Contribution My group provides knowledge, resources and materials, and (genome/gene) sequence information of various species of aphids.
Collaborator Contribution Huang's group provides information on insect transformation methods, insect sex determination genes, baculovirus and insect cell-based expression systems.
Impact We excnahed visits and attended (CEPAMS) meetings to discuss research progress. Qun Liu, Yazhou Chen and Weijie Huang, former postdoctoral researchers and PhD students at SIPPE, are currently postdoctoral researchers in the Hogenhout group.
Start Year 2014
 
Description Partnership award with Matthew Baylis, University of Liverpool, UK. 
Organisation University of Liverpool
Department School of Veterinary Science Liverpool
Country United Kingdom 
Sector Academic/University 
PI Contribution Provide information on vector-borne diseases of plants.
Collaborator Contribution Provide information of vector-borne diseases of humans and animals.
Impact We won a US Partnering Award: Vector-borne diseases in the UK & US: common threats and shared solutions" [Matthew Baylis, PI (PI, University of Liverpool), Saskia Hogenhout (co-PI, JIC) and Simon Carpenter (co-PI, The Pirbright Institute)] BBSRC £50k, and co-organized visits of US colleagues to the UK (Dec 2016) and UK group members to the University of California, Davis (Oct 2017). We applied for a GCRF VBD network grant (£4M) together; the pre-proposal for this was selected for submission of a full proposal, and we were invited for an interview with BBSRC based on our full proposal submission. The proposal was ranked 5th out 12 proposals, and only the top 3 were funded. Finally, we are co-organizing Vector-Borne Diseases in the UK meeting, 3-4 Dec 2018 that will be hosted at the JIC.
Start Year 2016
 
Description Partnership with Dr. Nicola Spence and Dr. Mike Sutton-Croft, Defra 
Organisation Department For Environment, Food And Rural Affairs (DEFRA)
Country United Kingdom 
Sector Public 
PI Contribution I engaged with Drs. Spence and Sutton-Croft to develop the BRIGIT proposal. We are currently working on response policies in the event Xylella fastidiosa is detected in the UK and strategies for integration of outcomes of the BRIGIT project in these reponse policies.
Collaborator Contribution Contribution of a wide range of knowledge on policy making and (methods for) collection of data for the BRIGIT project.
Impact We have weekly meetings when Dr. Sutton-Croft is at the John Innes Centre at one day per week.
Start Year 2018
 
Description AMM at APS, Minneapolis, USA 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact A postdoc presented an invited talk in teh session Interconnected lifecycles: multitrophic interactions between plants, pathogens and insects at the American Phytopathological Society (APS) - Canadian Phytopathological Society Joint Meeting, Minneapolis, USA, 9-13 August 2014. The talk sparked questions and discussion afterwards.
Year(s) Of Engagement Activity 2014
 
Description APS, Tampa, FL, USA 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Presented a research talk about my lab's recent progress made in functional analyses of phytoplasma effector proteins and how they change plant development and plant susceptibility to insect pests at the American Plant Pathology Society meeting, Tampa, Florida.
Year(s) Of Engagement Activity 2016
 
Description Annual Symposium Lausanne, Switserland 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Presented a seminar in the Annual Symposium Department of Fundamental Microbiology, University of Lausanne, Lausanne, Switzerland, 7 Nov 2014. This sparked questions and discussion afterwards. I engaged with leading and young scientists to discuss research.
Year(s) Of Engagement Activity 2014
 
Description Attended meeting at Bill and Melinda Gates foundation 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Supporters
Results and Impact Attended a meeting and presented a poster at the Grand Challenges; Explorations Agricultural Development (Round 8 & 9) and Nutrition (Round 7 & 8) Convening, Bill and Melinda Gates foundation, Seattle, Washington, USA, May 2013. Engaged with African scientists and assessed if the technology produced in this project is useful for applications to protect crops from whitefly pests and research of colleagues.
Year(s) Of Engagement Activity 2013
 
Description BSCD and BSDB 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Presented a research talk about my lab's recent progress made in functional analyses of phytoplasma effector proteins and how they change plant development and plant susceptibility to insect pests.
Year(s) Of Engagement Activity 2016
 
Description Biotechnology conference, Cuba 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Presented a talk in a parallel session at the Biotechnology Conference, Havana, Cuba, 1-5 Dec 2014. This sparked questions and discussion afterwards.
Year(s) Of Engagement Activity 2014
 
Description COST meeting, Copenhagen, Denmark 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I have a talk at the Plant-Insect-Microbe Interactions Symposium, Copenhagen, Denmark, 16 April 2015. This sparked questions and discussion afterwards.
Year(s) Of Engagement Activity 2015
 
Description Chair of Insectary Platform Steering Committee 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact Chair of committee that ensures that the JIC Insectary/Entomology Technology Platform is well managed, proactive, effective, state of the art and resourced to meet the Institutes' science needs.
Year(s) Of Engagement Activity 2017,2018,2019
 
Description Chair of Technical Platform Oversight Committee (TPOC) 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact Chair the committee that ensures that all JIC Technology Platforms are collectively well managed, proactive, effective, state of the art and resourced to meet the Institutes' science needs.
Year(s) Of Engagement Activity 2017,2018,2019
 
Description Chair of the Library and Information Services Steering Group, JIC 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact Make decisions on Library and Information Services at JIC.
Year(s) Of Engagement Activity 2013,2014,2015,2016,2017
 
Description Chaired session at IPMB, Iguassu Falls, Argentina 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Organized and chaired a parallel session on plant-insect interactions at the International Plant Molecular Biology 2015 meeting, Iguassu Falls, Argentina - Brazil, 25-30 October 2015. Established contacts with leaders in the field and engaged with students and postdoctoral researchers.
Year(s) Of Engagement Activity 2015
 
Description Co-chaired session at MPMI meeting, Rhodes, Greece 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Co-chaired concurrent session Neglected Systems at the International Congress of Molecular Plant-Microbe Interactions (MPMI), Rhodes, Greece, 6-10 July 2014. I established contacts with leaders in the MPMI field and engaged with students and postdoctoral scientists.
Year(s) Of Engagement Activity 2014
 
Description Co-chaired session at SEB, Manchester, UK 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I organized a co-chaired the session Mechanisms of Plant-Insect Interactions at the Society of Experimental Biology (SEB) meeting, Manchester, UK, 1-5 July 2014. I invited colleagues who are leaders in the field of Plant-Insect Interactions from academic institutions worldwide to present talks in this session. A student and a postdoc in my team presented talks as well and engaged with the international speakers and the audience.
Year(s) Of Engagement Activity 2014
 
Description Communication of research findings via Natural History magazine 
Form Of Engagement Activity A magazine, newsletter or online publication
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Media (as a channel to the public)
Results and Impact MacLean et al. (2014). PLoS Biol. 12: e1001835 was featured in Natural History magazine (published in the United States; mission: to promote public understanding and appreciation of nature and science).
Year(s) Of Engagement Activity 2014
 
Description Communication of research findings via The Economist 
Form Of Engagement Activity A magazine, newsletter or online publication
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact MacLean et al. (2014). PLoS Biol. 12: e1001835 was featured in the Science and Technology section of the print edition of The Economist: 'Symbiosis and parasitism: The sorceress's apprentice'. (12 April 2014).
Year(s) Of Engagement Activity 2014
 
Description Communication of research findings via international newspapers 
Form Of Engagement Activity A magazine, newsletter or online publication
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact MacLean et al. (2014). PLoS Biol. 12: e1001835 was highlighted in on-line national media of China (China today), Japan, Russia, USA, Vietnam, Indonesia, Greece, Italy, Spain, France, Germany, The Netherlands (Scientias; Nieuws.nl; Quest; Acksmedia) and UK (Daily Mail; EDP24).
Year(s) Of Engagement Activity 2014
 
Description Communication of research findings via local newspapers 
Form Of Engagement Activity A magazine, newsletter or online publication
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact MacLean et al. (2014). PLoS Biol. 12: e1001835 was featured in printed editions of the Eastern Daily Press (9 April 2014) and Evening News (9 April 2014): 'The zombie invasion really is coming'. These articles were advertised on billboards 'Norwich zombie plants riddle' in front of news agencies in Norwich.
Year(s) Of Engagement Activity 2014
 
Description Communication of research findings via radio 
Form Of Engagement Activity A broadcast e.g. TV/radio/film/podcast (other than news/press)
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact We contributed to Radio: NTR de Kennis van nu, radio 5, The Netherlands (Podcast April 30 2014 by Richard Immink); Moment of Science, Indiana Public Media, USA (broadcasted 2nd week of May 2014).
Year(s) Of Engagement Activity 2014
 
Description Communication of research findings via various websites and on-line media 
Form Of Engagement Activity Engagement focused website, blog or social media channel
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact MacLean et al. (2014). PLoS Biol. 12: e1001835 was highlighted on various websites of international media: Nature News; Scientific American; New York Times; Hortidaily (online meeting place for the international horticulture industry) with 2-min video of JIC; Students Science (Society of Science); Science 2.0; Science, Space & Robots; I fucking love science; Otters and Science News; Fearnet; Engadget.
Year(s) Of Engagement Activity 2014
 
Description Communication of research findings via video and press release 
Form Of Engagement Activity A broadcast e.g. TV/radio/film/podcast (other than news/press)
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Media (as a channel to the public)
Results and Impact We produced a 2-minute video at John Innes Centre (Chris Wilson) to communicate research of MacLean et al. (2014). PLoS Biol. 12: e1001835 to a general audience - released 8 April 2014 along with written press releases of John Innes Centre (Zoe Dunford) and Wageningen University and Research Centre (Erik Toussaint).
Year(s) Of Engagement Activity 2014
 
Description Contributed an 'Accessible Science Talk' for the Research and Support Staff Voice (RSSV) 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact The JIC Research and Support Staff Voice (RSSV) organised a day (9 Oct 2018) of talks as part of the JIC Annual Science Meeting, delivered by top scientists from JIC and TSL. Every talk was accessible to a wide audience including scientists, support and partnership staff and communicates the great work carried out at JIC and TSL, presented in a manner that will be understandable and interesting for all.
Year(s) Of Engagement Activity 2018
 
Description Contributed to a press release 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Media (as a channel to the public)
Results and Impact Contributed to a press release to announce funding of this project from Bill and Melinda Gates Foundation Grand Challenges Explorations Program (2013).
Year(s) Of Engagement Activity 2013
 
Description Contribution of two lectures for BIO-3C20 Molecular Plant-Microbe Interactions course 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Undergraduate students
Results and Impact Postdoctoral researcher Adi Kliot in my lab will contribute two lectures in the BIO-3C20 Molecular Plant-Microbe Interactions, Spring Semester, University of East Anglia, March 2019.
Year(s) Of Engagement Activity 2019
 
Description EPS, Soest 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact I presented a talk at the Dutch Graduate School Experimental Plant Sciences (EPS), Soest, The Netherlands (invited by PhD students), 29-30 Jan 2015. The audience was predominantly postgraduate students.
Year(s) Of Engagement Activity 2015
 
Description Engaged with journalists from Africa 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Media (as a channel to the public)
Results and Impact Engagement with the general public: Presented talk for group of African journalists visiting the JIC, Apr. 2014
Year(s) Of Engagement Activity 2014
 
Description Engagement with BBSRC as part of the BRIGIT project 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Policymakers/politicians
Results and Impact Have weekly meetings with policy makers at BBSRC about new policies that may be developed to reduce the risk of Xylella fastidiosa outbreaks in the UK
Year(s) Of Engagement Activity 2018,2019
 
Description Engagement with Defra, Forest Research and APHA as part of the BRIGIT project 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Policymakers/politicians
Results and Impact Have weekly meetings with policy makers at Defra about new policies that may be developed to reduce the risk of Xylella fastidiosa outbreaks in the UK
Year(s) Of Engagement Activity 2018,2019
 
Description Engagement with Fera as part of the BRIGIT project 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Have weekly meetings with Fera about status of Xylella fastidiosa diagnostics that may influence new policies for reducing risk of X. fastidiosa outbreaks in the UK
Year(s) Of Engagement Activity 2018,2019
 
Description Engagement with Oxitec 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Regular meetings with Neil Morrison for supervisory meetings and discussing research progress of the iCASE studentship.
Year(s) Of Engagement Activity 2015,2016,2017,2018,2019
 
Description Engagement with SV 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact Three-monthly meetings with industrial collaborators
Year(s) Of Engagement Activity 2017,2018,2019
 
Description Engagement with Syngenta 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact Regular meetings with colleagues at Syngenta, Jealott's Hill, UK, and Switserland and USA to discuss project proposals and research progress on aphids.
Year(s) Of Engagement Activity 2010,2011,2012,2013,2014
 
Description Engagement with the Royal Horticulticultural Society as part of the BRIGIT project 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Third sector organisations
Results and Impact Have weekly meetings with staff member of RHS to develop a stakeholder engagement plan for BRIGIT
Year(s) Of Engagement Activity 2018,2019
 
Description GMI, Vienna 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Presented a research talk about my lab's recent progress made in functional analyses of phytoplasma effector proteins and how they change plant development and plant susceptibility to insect pests at the Gregor Mendel Institute, Vienna, Austria.
Year(s) Of Engagement Activity 2016
 
Description HFSP meeting, SIngapore 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Policymakers/politicians
Results and Impact Presented a research talk about my lab's recent progress made in functional analyses of phytoplasma effector proteins and how they change plant development and plant susceptibility to insect pests at the Human Frontier Science Program (HFSP) meeting, Singapore.
Year(s) Of Engagement Activity 2016
 
Description Hosted Alexander Stacey, BSc student Biological Sciences, University of East Anglia, UK. 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Undergraduate students
Results and Impact Student worked on a research project in the summer of 2018 and his BSc research project from Jan-Mar '19.
Year(s) Of Engagement Activity 2018,2019
 
Description Hosted Alexandra Kolodyazhnaya, BSc student Novosibirsk State University, Russia 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Undergraduate students
Results and Impact Student did a research project in my lab as part of the JIC/TSL/EI International Undergraduate Summer School programme at JIC.
Year(s) Of Engagement Activity 2018
 
Description Hosted Libby Hanwell, BSc student Biological Sciences, University of East Anglia, UK 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Undergraduate students
Results and Impact Hosted the student for a research project in the summer of 2018 and her BSc research project from Oct-Dec '18
Year(s) Of Engagement Activity 2018,2019
 
Description Hosted Luke Sherwin, MSc student Molecular Medicine at the University of East Anglia, UK. 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Postgraduate students
Results and Impact MSc project for BIO_6019Y course. Luke wrote a 3000-word project and did a research project Mar-Sep 2018
Year(s) Of Engagement Activity 2018
 
Description Hosting of Milind Sood, BSc student, University of Oxford, UK. 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Undergraduate students
Results and Impact Will do a research project in my lab as part of the JIC/TSL/EI International Undergraduate Summer School programme at JIC, Jun-Aug 2019.
Year(s) Of Engagement Activity 2019
 
Description ICE, Florida, USA 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Presented an invited talk at the International Conference of Entomology (ICE), Orlando, Florida, USA.
Year(s) Of Engagement Activity 2016
 
Description IMPRS, Jena 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Presented a keynote lecture at the 16th IMPRS Symposium (organized by PhD students at Max Planck Institute), Donburg Castle, Jena, Germany.
Year(s) Of Engagement Activity 2017
 
Description Invited research seminar at 'When Development meets Stress' symposium, CRAG, Barcelona, Spain 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Presented a talk on Phytoplasma at the 'When Development meets Stress' symposium, Center for Research in Agricultural Genomics (CRAG), Barcelona, Spain, 2-4 Sep 2018.
Year(s) Of Engagement Activity 2018
URL https://www.bdebate.org/en/forum/when-development-meets-stress-understanding-developmental-reprogram...
 
Description Invited research seminar at CAS institute 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Presented a research seminar at IGDB, Beijing, China on 16 Mar 2018. Research fellow Thomas Mathers in my lab also contributed a talk.
Year(s) Of Engagement Activity 2018
 
Description Invited research seminar at iDiv, Leipzig, Germany 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I was invited by PhD students to give a research presentation at the Integrative Biodiversity Research Institute (iDiv), Halle-Jena-Leipzig, Germany, 18 Apr 2018. I was hosted by Crispus Mbaluto, PhD student at iDiv.
Year(s) Of Engagement Activity 2018
 
Description Invited research seminar at the Max Planck Institute, Cologne, Germany 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I was invited to give a research seminar at the Max Planck Institute for Plant Breeding, Cologne, Germany. Approximately 50 people, including PhD students, attended.
Year(s) Of Engagement Activity 2018
 
Description Invited research seminar at the XI European Congress of Entomology, Naples, Italy 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Presented a research seminar on Phytoplasma in a parallel session focused on insect-transmitted plant pathogens and plant-insect interactions.
Year(s) Of Engagement Activity 2018
 
Description Invited research seminar at the annual Life Sciences conference, Beijing, China 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Presented a talk in a paralell session focused on insect pests at the Life Sciences Conference, Beijing, China, 28-31 Oct '19.
Year(s) Of Engagement Activity 2018
 
Description MCC at SEB, Manchester, UK 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact A visiting postdoc in the lab presented a talk in the parallel session Mechanisms of Plant-Insect Interactions at the Society of Experimental Biology (SEB) meeting, Manchester, UK, 1-5 July 2014
Year(s) Of Engagement Activity 2014
 
Description Marburg, Germany 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Presented an invited talk at the Frontiers in Microbiology Symposium, Max Planck Institute, Marburg, Germany.
Year(s) Of Engagement Activity 2016
 
Description Member Scientific Resources Committee 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact Member of a committee that decides on purchases of all types of scientific equipment and organization of JIC infrastructure.
Year(s) Of Engagement Activity 2017,2018,2019
 
Description Member of Programme Management Group of JIC Next Generation Infrastructure 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact Feb 2018 - current Member of Programme Management Group of JIC Next Generation Infrastructure
Year(s) Of Engagement Activity 2018,2019
 
Description Member of Tenure-Track faculty mentoring committee 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact Provide advise to young faculty
Year(s) Of Engagement Activity 2016,2017,2018,2019
 
Description Member of supervisory committee of PhD student at University of Wisconsin, Madison, USA 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Aug 2018 - current: Member of supervisory committee of PhD student Shannon Piper, University of Madison, Wisconsin, USA.
Year(s) Of Engagement Activity 2018,2019
 
Description Member of the JIC Distribution of Exploitation Income (DEI) Panel 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact Helped to decide on the Distribution of Exploitation Income of colleages at the Norwich Research Park
Year(s) Of Engagement Activity 2012,2013,2014,2015,2016
 
Description OARDC, Wooster, OH, USA 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I presented a talk at my former workplace: The Ohio State University - Ohio Agriculture and Development Center (OARDC), Wooster, Ohio, USA. 10-13 May 2015. This sparked questions and discussions afterwards.
Year(s) Of Engagement Activity 2015
 
Description Organizing the satellite meeting 'Dynamics and Mechanism of Insect-Transmitted Pathogens' at the IS-MPMI 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact This satellite meeting will be organized on Sunday 14th July, 8.30 - 15.00, and brings together scientists with an interest in studying the diverse aspects of insect-transmitted plant pathogen interactions with their plant hosts and insect vectors. These pathogens cause the most destructive diseases of plants and are often responsible for eradication of entire crop industries in countries or regions. Current examples include Olive Quick Decline Syndrome (Xylella fastidiosa), citrus greening (Candidatus Liberibacter sp.), Lime Witch's Broom and Grapevine Flavescence doree (Candidatus Phytoplasma) and Cassava mosaic (geminivirus). The meeting will cover (1) Recognition and induction of immunity, (2) Effectors and modulation of insect/plant processes, (3) Epidemiology and insect transmission and (4) Genomics and Evolution. If you would like to attend or contribute to the meeting, please contact Saskia Hogenhout, Silke Robatzek, Sheng-Yang He and Steven Lindow by 15 April 2019 at the very latest.
Year(s) Of Engagement Activity 2018,2019
URL https://www.ismpmi.org/Congress/2019/program/Pages/Satellite-Meetings.aspx
 
Description Phytopathological Society, Colmar, France 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I presented a talk at the French Phytopathology Meeting, Colmar, France, 2-5 June 2015. This sparked questions and discussion afterwards.
Year(s) Of Engagement Activity 2015
 
Description Phytopathology conference, Brazil 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact SH presented a talk at the 46th Meeting of the Brazilian Phytopathological Society/11th Brazilian Meeting on Biological Control, Ouro Preto, Brazil, 20-25 October 2013.
Year(s) Of Engagement Activity 2013
 
Description Presented a talk at Monogram 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Presented a talk at Monogram, JIC, Norwich, UK, 24-16 Apr 2018.
Year(s) Of Engagement Activity 2018
 
Description Presented a talk at a whitefly conference 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Presented a talk (invited based on abstract submission) at the 1st International Whitefly Symposium, Kolymbari, Sicily, Italy, May 2013.
Year(s) Of Engagement Activity 2013
 
Description Press release to announce BRIGIT project 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Media (as a channel to the public)
Results and Impact Engaged with JIC Public Engagement Officer to launch press release 'UK consortium to combat serious threat to plant health' to announce the start of the BRIGIT project and the BRIGIT project website
Year(s) Of Engagement Activity 2018
URL https://www.jic.ac.uk/press-release/uk-wide-consortium-to-combat-serious-threat-to-plant-health/
 
Description Press release to announce new appointment for the BRIGIT project 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Media (as a channel to the public)
Results and Impact Engaged with JIC Public Engagement Officer to launch press release 'New appointment for UK-wide Xylella pathogen consortium' for the BRIGIT project
Year(s) Of Engagement Activity 2019
URL https://www.jic.ac.uk/news/new-appointment-for-uk-wide-xylella-pathogen-consortium/
 
Description UK Microbiology Society 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Presented a research talk about my lab's recent progress made in functional analyses of phytoplasma effector proteins and how they change plant development and plant susceptibility to insect pests.
Year(s) Of Engagement Activity 2016