Elucidating and engineering natural product biosynthesis in actinomycetes
Lead Research Organisation:
John Innes Centre
Department Name: Contracts Office
Abstract
My research encompasses the discovery, characterisation and engineering of pathways that make drug-like molecules in actinomycete bacteria. I am particularly interested in bioactive natural products that also possess unusual structural features. We employ a "gene to product" approach, which requires a wide variety of techniques, such as the computational analysis of bacterial genomes, the genetic manipulation of gene clusters and the in vitro analysis of pathway proteins.
Two major routes to peptide natural products have evolved: non-ribosomal peptide synthetases (NRPSs) and ribosomally synthesized and post-translationally modified peptides (RiPPs). We study the biosynthesis of complex metabolites produced by both types of pathway. NRPSs are massive multi-domain assembly line proteins and are responsible for the biosynthesis of the glycopeptide antibiotics. These hugely complex molecules are used as drugs of last resort to treat aggressive methicillin-resistant Staphylococcus aureus (MRSA) infections. Through a variety of collaborations, I am investigating the regulation, activity and biosynthesis of these molecules. In contrast to NRPSs, RiPPs originate from ribosomal precursor peptides. Bottromycin is a clinically promising and structurally unique RiPP, and is active towards multi-drug resistant bacteria, such as MRSA. Its structurally novelty makes it a promising lead compound in the fight against infection. Following the discovery of the bottromycin pathway in Streptomyces scabies, we are now characterising the enzymatic steps of this pathway to determine how structural complexity is introduced into this compound. Additional work on this pathway includes mutagenesis to produce novel derivatives and an analysis of the regulatory factors that control bottromycin biosynthesis. This research will inform future investigations into other novel RiPP pathways.
Two major routes to peptide natural products have evolved: non-ribosomal peptide synthetases (NRPSs) and ribosomally synthesized and post-translationally modified peptides (RiPPs). We study the biosynthesis of complex metabolites produced by both types of pathway. NRPSs are massive multi-domain assembly line proteins and are responsible for the biosynthesis of the glycopeptide antibiotics. These hugely complex molecules are used as drugs of last resort to treat aggressive methicillin-resistant Staphylococcus aureus (MRSA) infections. Through a variety of collaborations, I am investigating the regulation, activity and biosynthesis of these molecules. In contrast to NRPSs, RiPPs originate from ribosomal precursor peptides. Bottromycin is a clinically promising and structurally unique RiPP, and is active towards multi-drug resistant bacteria, such as MRSA. Its structurally novelty makes it a promising lead compound in the fight against infection. Following the discovery of the bottromycin pathway in Streptomyces scabies, we are now characterising the enzymatic steps of this pathway to determine how structural complexity is introduced into this compound. Additional work on this pathway includes mutagenesis to produce novel derivatives and an analysis of the regulatory factors that control bottromycin biosynthesis. This research will inform future investigations into other novel RiPP pathways.
Organisations
- John Innes Centre, United Kingdom (Lead Research Organisation)
- Earlham Institute, NORWICH (Collaboration)
- California State University, Bakersfield (Collaboration)
- University of Calabria, Italy (Collaboration)
- Helmholtz Association of German Research Centres (Collaboration)
- Fundacion MEDINA (Collaboration)
- University of Cambridge (Collaboration)
- University of Lviv (Collaboration)
- Commonwealth Scientific and Industrial Research Organisation (Collaboration)
- University of Zagreb (Collaboration)
- University of Leon (Collaboration)
- VCS Potatoes (Collaboration)
- Eberhard Karls University Tuebingen, Germany (Collaboration)
People |
ORCID iD |
| Andrew William Truman (Principal Investigator) |
Publications
Crone W
(2016)
Dissecting Bottromycin Biosynthesis Using Comparative Untargeted Metabolomics
in Angewandte Chemie
Crone WJ
(2016)
Dissecting Bottromycin Biosynthesis Using Comparative Untargeted Metabolomics.
in Angewandte Chemie (International ed. in English)
Eyles TH
(2018)
Rapid and Robust Yeast-Mediated Pathway Refactoring Generates Multiple New Bottromycin-Related Metabolites.
in ACS synthetic biology
Franz L
(2017)
Macroamidine Formation in Bottromycins Is Catalyzed by a Divergent YcaO Enzyme
in Journal of the American Chemical Society
Frattaruolo L
(2017)
A Genomics-Based Approach Identifies a Thioviridamide-Like Compound with Selective Anticancer Activity.
in ACS chemical biology
Horbal L
(2014)
The pathway-specific regulatory genes, tei15* and tei16*, are the master switches of teicoplanin production in Actinoplanes teichomyceticus.
in Applied microbiology and biotechnology
Leipoldt F
(2017)
Warhead biosynthesis and the origin of structural diversity in hydroxamate metalloproteinase inhibitors
in Nature Communications
Medema MH
(2015)
Minimum Information about a Biosynthetic Gene cluster.
in Nature chemical biology
Miethke M
(2021)
Towards the sustainable discovery and development of new antibiotics.
in Nature reviews. Chemistry
Montalbán-López M
(2021)
New developments in RiPP discovery, enzymology and engineering.
in Natural product reports
| Description | This research grant has helped support research across a variety of projects relating to the biosynthesis of bacterial natural products. This includes understanding how potent antibiotics are made, including bottromycin (http://onlinelibrary.wiley.com/doi/10.1002/anie.201604304/abstract), teicoplanin (https://pubs.acs.org/doi/10.1021/acschembio.6b00018 , https://link.springer.com/article/10.1007%2Fs00253-014-5969-z), actinonin and matlystatin (https://www.nature.com/articles/s41467-017-01975-6). Additional work has focused on the discovery of biosynthetic pathways to new antibacterial and anticancer compounds, including ristocetin (http://aac.asm.org/content/58/10/5687.long) and thioviridamide-like molecules (http://pubs.acs.org/doi/10.1021/acschembio.7b00677). This reflects a significant number of diverse natural products with potent biological activities, and this work provides information on how these molecules are made, including some very unusual enzymatic transformations. More fundamentally, we have been able to develop methodology that is assisting with the chemical analysis of natural products. |
| Exploitation Route | We have identified a wide array of interesting biosynthetic pathways that will be of significant interest to the natural products community, as well as researchers in biocatalysis and microbiology. This could include structural characterisation of enzymes, employment of enzymes as biocatalysts for challenging chemical reactions, analysis of the mechanism of action of some of these natural products, or the exploitation of the methodology that we report on other areas of natural products research. |
| Sectors | Agriculture, Food and Drink,Chemicals,Healthcare,Manufacturing, including Industrial Biotechology,Pharmaceuticals and Medical Biotechnology |
| Description | Methodology supported by this grant led to the establishment of a collaboration with a local company (Celbius Ltd) to assess whether sonication can aid in natural product yield and/or ease of extraction. This was supported by an NPRONET Proof of Concept grant (code POC005_2) and was reported at an NPRONET meeting (Manchester, Oct 2016) to a mixed audience of academia and industry. Here, we did show that low-power sonication led to substantial changes in metabolic profiles of multiple actinobacterial strains (without affecting growth rates). Another collaboration (joint with Jake Malone, John Innes Centre) was established with a local company (VCS Potatoes Ltd) to assess the ability of soil-dwelling bacteria to control important plant diseases. This work has led to new methodology to assess bacterial soil populations and their ability to suppress plant diseases, as well as the establishment of further biocontrol projects with other companies (led by Jake Malone). This work was reported to industry members at Science for Innovation Showcase event at the John Innes Centre, where we subsequently discussed opportunities for large-scale field trials with a variety of interested parties. The biocontrol collaboration with Jake Malone has led to work with a UK-based biotechnology company, who aim to develop biocontrol strains based on the bacteria that we have isolated and studied. Field trials to assess the capacity of our strains to inhibit potato pathogens were carried out in 2020. Following the publication of our work in late 2021, we have been communicating with a number of farmers and agri-tech companies about further field trials. |
| First Year Of Impact | 2015 |
| Sector | Agriculture, Food and Drink,Manufacturing, including Industrial Biotechology,Pharmaceuticals and Medical Biotechnology |
| Impact Types | Economic |
| Description | Contribution to SAW Antibiotics book |
| Geographic Reach | National |
| Policy Influence Type | Influenced training of practitioners or researchers |
| URL | http://www.sawtrust.org/buy-the-books/saw-antibiotics/ |
| Description | Discovery of natural products that are critical for controlling plant pathogens (TRUMAN_J17DTP) |
| Amount | £95,000 (GBP) |
| Funding ID | 1937478 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 09/2017 |
| End | 09/2021 |
| Description | Harnessing the biosynthetic potential of bacteria to produce ribosomally synthesised natural products |
| Amount | £554,967 (GBP) |
| Funding ID | BB/V016024/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 08/2021 |
| End | 09/2024 |
| Description | NPRONET Proof of Concept funding |
| Amount | £54,342 (GBP) |
| Funding ID | NPRONET POC005_2 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 01/2016 |
| End | 05/2016 |
| Description | NRP Science Links Seed Corn funding |
| Amount | £15,232 (GBP) |
| Organisation | Norwich Research Park |
| Sector | Private |
| Country | United Kingdom |
| Start | 03/2015 |
| End | 10/2015 |
| Description | Norwich Research Park BBSRC DTP PhD Studentship |
| Amount | £95,000 (GBP) |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 09/2016 |
| End | 09/2020 |
| Description | Royal Society Enhancement Award |
| Amount | £99,859 (GBP) |
| Funding ID | RGF/EA/180154 |
| Organisation | The Royal Society |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 12/2017 |
| End | 03/2021 |
| Description | Royal Society Enhancement Award |
| Amount | £170,640 (GBP) |
| Funding ID | RGF\EA\181083 |
| Organisation | The Royal Society |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 09/2018 |
| End | 03/2021 |
| Description | Royal Society University Research Fellowships Renewal |
| Amount | £363,410 (GBP) |
| Funding ID | URF\R\180007 |
| Organisation | The Royal Society |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 09/2018 |
| End | 09/2021 |
| Description | UK China AMR Call (Newton Fund) |
| Amount | £490,718 (GBP) |
| Funding ID | MR/P007570/1 |
| Organisation | Medical Research Council (MRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 06/2016 |
| End | 06/2019 |
| Title | RIPPER genome mining |
| Description | RiPPER is a command line computational tool that assists in the identification of biosynthetic gene clusters and associated precursor peptides for RiPPs, a large and important class of natural product. |
| Type Of Material | Improvements to research infrastructure |
| Year Produced | 2018 |
| Provided To Others? | Yes |
| Impact | This has assisted in research projects in my group and I have had contact with researchers in other groups who have benefited from the tool and the results we have reported from its use. It assisted in the identification of a new family of natural products, the thiovarsolins, which were characterised by my group. The identification of these natural products and the development of the tool were reported in a BioRxiv pre-print (https://www.biorxiv.org/content/10.1101/494286v1) and have just been accepted for publication in Nucleic Acids Research. |
| URL | https://github.com/streptomyces/ripper |
| Title | YcaO Biosynthetic Gene Clusters in Actinobacteria |
| Description | 1,464 GenBank files retrieved and annotated by RiPPER (https://github.com/streptomyces/ripper; RiPPER originally reported in https://academic.oup.com/nar/article/47/9/4624/5420534) using standalone YcaO proteins from Actinobacteria as the input. This dataset also includes 6 entries from Firmicutes that are reported in the associated manuscript (see reference below). The GenBank files represent putative biosynthetic gene clusters associated with YcaO domain proteins. The files are named with the species name and the accession of the input YcaO protein. When viewed in the Artemis genome browser (Wellcome Sanger Institute), the input YcaO protein is coloured green and short peptides ( |
| Type Of Material | Database/Collection of data |
| Year Produced | 2021 |
| Provided To Others? | Yes |
| Impact | Enquiries from potential collaborators. |
| URL | https://figshare.com/articles/dataset/YcaO_Biosynthetic_Gene_Clusters_in_Actinobacteria/14191544/1 |
| Description | Actinobacterial plant pathogen |
| Organisation | California State University, Bakersfield |
| Country | United States |
| Sector | Academic/University |
| PI Contribution | We are investigating natural products associated with a plant pathogen and their role in pathogenicity. |
| Collaborator Contribution | The partner has generated bacterial mutants and provided expertise in plant pathogenicity assays. |
| Impact | Research is ongoing. Multidisciplinary: plant pathology, genetics, microbiology, chemistry |
| Start Year | 2020 |
| Description | Agrobacterium pathway discovery |
| Organisation | University of Zagreb |
| Country | Croatia |
| Sector | Academic/University |
| PI Contribution | 1. Host laboratory for research visit (enzymology, chemical synthesis, mass spectrometry) 2. Input into design of experiments and analysis of data. |
| Collaborator Contribution | 1. Started project and designed experiments. 2. Prepared materials (enzymes, substrates) for project. |
| Impact | Multidisciplinary: microbiology, biochemistry, chemistry |
| Start Year | 2014 |
| Description | CSIRO collaboration |
| Organisation | Commonwealth Scientific and Industrial Research Organisation |
| Department | CSIRO Black Mountain Laboratories |
| Country | Australia |
| Sector | Private |
| PI Contribution | My research group hosted a senior researcher for a collaborative research project associated with microbe-plant and microbe-microbe interactions, with a particular focus on Streptomyces strains with inhibitory activity towards fungal pathogens of plants. We provided expertise in Streptomyces genetics, natural product biosynthesis and mass spectrometry. |
| Collaborator Contribution | The CSIRO researcher provided Streptomyces strains with biocontrol activity, as well as extensive expertise in fungal pathogens of crop plants. The bench work for this collaboration was carried out by the CSIRO researcher while they visited JIC. The research visit was for 4 months, but the collaboration is still ongoing. |
| Impact | Manuscript is currently under review. Australian funding: CERC Postdoctoral Fellowship Multi-disciplinary colaboration: microbiology, fungal biology, natural products chemistry, mass spectrometry, plant pathology. |
| Start Year | 2019 |
| Description | Calabria thioviridamides |
| Organisation | University of Calabria |
| Country | Italy |
| Sector | Academic/University |
| PI Contribution | We established a project to discovery new thioviridamide-like molecules (TLMs) by the use of a genome mining method. This involved pathway identification, strain fermentation, pathway cloning and mutagenesis, and then purification and chemical analysis of the products of these pathways. |
| Collaborator Contribution | The group of Anna Rita Cappello determined the biological activity of our purified compounds against bacteria, fungi and human cell lines. |
| Impact | Publication: https://pubs.acs.org/doi/10.1021/acschembio.7b00677 This collaboration is multi-disciplinary. We carry out microbiology, genetics and chemistry and the partners carry out cell biology assays. |
| Start Year | 2016 |
| Description | Earlham Institute Biofoundry |
| Organisation | Earlham Institute |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Development of an assay for strains that inhibit the growth of the plant pathogen Streptomyces scabies |
| Collaborator Contribution | Technical insight and method development relating to the use of a robotic system for the high-throughput screening of Streptomyces inhibition. |
| Impact | Paper: Moffat, A. D., Elliston, A., Patron, N. J., Truman, A. W. & Carrasco Lopez, J. A. A biofoundry workflow for the identification of genetic determinants of microbial growth inhibition. Synth Biol, 6, ysab004 (2021). |
| Start Year | 2018 |
| Description | Glycopeptide discovery and activity |
| Organisation | University of Cambridge |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | 1. Isolation and identification of ristocetin from an Amycolatopsis producing strain. 2. Analysis of the ristocetin gene cluster. 3. Analysis of the vancomycin-zinc interaction alongside an interactions D-Ala-D-Ala and D-Ala-D-Lac using NMR and ITC. 4. Design of experiments and input into manuscript preparation. |
| Collaborator Contribution | 1. Project design. 2. Mutagenesis of actinomycetes for glycopeptide discovery and activity analysis. 3. Transcriptional analysis and bioassays with Streptomyces coelicolor. 4. Manuscript writing. |
| Impact | PubmedID: 25022591 PubmedID: 26797186 Multidisciplinary: chemistry, biochemistry, microbiology |
| Start Year | 2013 |
| Description | HIPS Bottromycin Biosynthesis |
| Organisation | Helmholtz Association of German Research Centres |
| Department | Helmholtz Institute for Pharmaceutical Research, Saarbrucken |
| Country | Germany |
| Sector | Academic/University |
| PI Contribution | Using tandem mass spectrometry, we characterised enzymatic transformations to the bottromycin precursor peptide. In addition, we generated mutant forms of the bottromycin producing organism (Streptomyces scabies) to determine the importance of a number of enzyme residues for catalysis. This was assessed by looking at the metabolites produced by these mutants using LC-MS. |
| Collaborator Contribution | They expressed and purified enzymes involved in bottromycin biosynthesis, and then carried out enzymatic assays. The products of these assays were then sent to us for analysis. They also carried out mutagenesis of these enzymes, which guided our mutations in Streptomyces scabies. |
| Impact | Publication: https://pubs.acs.org/doi/10.1021/jacs.7b09898 |
| Start Year | 2017 |
| Description | Teicoplanin biosynthesis |
| Organisation | Helmholtz Association of German Research Centres |
| Department | Helmholtz Institute for Pharmaceutical Research, Saarbrucken |
| Country | Germany |
| Sector | Academic/University |
| PI Contribution | 1. Analysis of mutants in the teicoplanin biosynthetic pathway. 2. Manuscript writing. 3. Input into project design. |
| Collaborator Contribution | 1. Genetic modification of teicoplanin gene cluster. 2. Transcriptional analysis of teicoplanin gene cluster. 2. Manuscript writing. 3. Project design. |
| Impact | PubmedID: 25104028 PubmedID: 27285718 Multidisciplinary: microbiology, analytical chemistry, molecular biology. |
| Start Year | 2013 |
| Description | Teicoplanin biosynthesis |
| Organisation | University of Lviv |
| Department | Department of genetics and biotechnology |
| Country | Ukraine |
| Sector | Academic/University |
| PI Contribution | 1. Analysis of mutants in the teicoplanin biosynthetic pathway. 2. Manuscript writing. 3. Input into project design. |
| Collaborator Contribution | 1. Genetic modification of teicoplanin gene cluster. 2. Transcriptional analysis of teicoplanin gene cluster. 2. Manuscript writing. 3. Project design. |
| Impact | PubmedID: 25104028 PubmedID: 27285718 Multidisciplinary: microbiology, analytical chemistry, molecular biology. |
| Start Year | 2013 |
| Description | University of León Collaboration |
| Organisation | Fundacion MEDINA |
| Country | Spain |
| Sector | Charity/Non Profit |
| PI Contribution | This collaboration was started to identify the natural products that were responsible for antifungal activity in Streptomyces clavuligerus following introduction of a regulatory gene into this strain. We also mapped the global changes to metabolism in this strain too using LC-MS. This led to the identification of a wide variety of tunicamycins, where a few specific versions of this molecule exhibited antifungal activity. We helped write the resulting research paper. |
| Collaborator Contribution | The partners led this research project, where they genetically manipulated Streptomyces clavuligerus and assessed changes to gene expression using microarrays. They also assessed the changes in production of other natural products known to be produced by Streptomyces clavuligerus , including the beta-lactam antibiotic cephamycin and the beta-lactamase inhibitor clavulanic acid. They led the writing of the research paper. |
| Impact | A paper was published in Frontiers in Microbiology that reports this work: https://www.frontiersin.org/articles/10.3389/fmicb.2019.00580/abstract |
| Start Year | 2016 |
| Description | University of León Collaboration |
| Organisation | University of Leon |
| Country | Spain |
| Sector | Academic/University |
| PI Contribution | This collaboration was started to identify the natural products that were responsible for antifungal activity in Streptomyces clavuligerus following introduction of a regulatory gene into this strain. We also mapped the global changes to metabolism in this strain too using LC-MS. This led to the identification of a wide variety of tunicamycins, where a few specific versions of this molecule exhibited antifungal activity. We helped write the resulting research paper. |
| Collaborator Contribution | The partners led this research project, where they genetically manipulated Streptomyces clavuligerus and assessed changes to gene expression using microarrays. They also assessed the changes in production of other natural products known to be produced by Streptomyces clavuligerus , including the beta-lactam antibiotic cephamycin and the beta-lactamase inhibitor clavulanic acid. They led the writing of the research paper. |
| Impact | A paper was published in Frontiers in Microbiology that reports this work: https://www.frontiersin.org/articles/10.3389/fmicb.2019.00580/abstract |
| Start Year | 2016 |
| Description | University of Tübingen Actinonin Biosynthesis |
| Organisation | Eberhard Karls University of Tübingen |
| Country | Germany |
| Sector | Academic/University |
| PI Contribution | We sequenced the genome of the actinonin producing organism to identify its biosynthetic gene cluster, as well as identifying the gene cluster of the related compound matlystatin in a different organism. We carried out chemical feeding experiments to determine the biosynthesis of actinonin and also carried out feeding experiments to make novel matlystatin derivatives. |
| Collaborator Contribution | They independently identified the same gene clusters as ourselves. Following this, they cloning the matlystatin gene cluster and investigated its biosynthesis by mutating most genes in the pathway. They also carried out different feeding experiments to ourselves to support biosynthetic proposals. |
| Impact | Publication: http://europepmc.org/abstract/med/29213087 |
| Start Year | 2017 |
| Description | VCS Potatoes |
| Organisation | VCS Potatoes |
| Country | United Kingdom |
| Sector | Private |
| PI Contribution | This is a collaboration to identify Pseudomonas strains that can control potato pathogens. Working with the group of Dr Jake Malone (JIC), we have carried out all scientific aspects of this project, including sampling and genome sequencing of isolated strains, as well as phenotyping, genetics and natural product analysis of these strains. |
| Collaborator Contribution | VCS Potatoes have provided sites for sampling and field trials. Their input also includes technical expertise relating to potato disease and field trials. |
| Impact | Publication: Pacheco-Moreno, A., Stefanato, F. L., Ford, J. J., Trippel, C., Uszkoreit, S., Ferrafiat, L., Grenga, L., Dickens, R., Kelly, N., Kingdon, A. D., Ambrosetti, L., Nepogodiev, S. A., Findlay, K. C., Cheema, J., Trick, M., Chandra, G., Tomalin, G., Malone, J. G. & Truman, A. W. Pan-genome analysis identifies intersecting roles for Pseudomonas specialized metabolites in potato pathogen inhibition. eLife 10, e71900 (2021). (originally available as a pre-print at bioRxiv: https://doi.org/10.1101/783258). Multidisciplinary: chemistry, informatics, microbiology, agriculture, plant trials, genetics |
| Start Year | 2015 |
| Description | SAW (Science, Art and Writing) Antibiotics Project at City of Norwich School. |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Schools |
| Results and Impact | Workshop with year 7 schoolchildren at a local school. I presented on microbes, antibiotics and disease, and the students then carried out creative writing and art projects based around the science. This was a full day project carried out alongside an artist, a writer, the schoolteacher and an outreach coordinator. The students prepared some fantastic poems and artwork. |
| Year(s) Of Engagement Activity | 2015 |
| Description | School visit from Ipswich High School |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Schools |
| Results and Impact | A small group of sixth-form students visited the research institute. I provided a tour of the labs and gave a presentation on antibiotics and Streptomyces bacteria. Following the visit, a student contacted me to obtain information on antibiotics for a school project. |
| Year(s) Of Engagement Activity | 2015 |
| Description | Antibiotic Hunters Exhibit at the Great British Biosciences Festival (London) |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Public/other audiences |
| Results and Impact | An exhibit to communicate the research we're doing to discover new antibiotics. This was a four-day festival, with VIPs on the first day, schools on the second (1500 pupils) and the public on the third and fourth (5000 in total). The exhibit was manned from researchers from a variety of groups in our department and a huge amount of interaction was made with the public. In connection with this, we prepared an animation to accompany the exhibit, which describes our research (linked in URL). |
| Year(s) Of Engagement Activity | 2014 |
| URL | https://www.youtube.com/watch?v=4tdK9VX5gcY |
| Description | Antibiotic Hunters Stand at Science in Norwich Day |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Public/other audiences |
| Results and Impact | Exhibit at a science fair in the centre of Norwich. Comprehensive feedback from the public was obtained by an independent reviewer to inform future outreach activities. Example feedback provided below: - Very many participants had a positive experience at the stand; none recorded a negative one. - Many responses noted the enthusiasm, knowledge, friendliness and approachability of all volunteers staffing the stand. - The relative simplicity of the materials at the stand, and the focus and clarity of the message, helped make the stand accessible. |
| Year(s) Of Engagement Activity | 2014 |
| Description | Antibiotic Hunters stand at the Big Bang Science Fair (Birmingham) |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Schools |
| Results and Impact | Outreach exhibit at a large national science fair attended by thousands of schoolchildren, as well as interested members of the public. Lots of interactions with schools and lots of requests for information about the research. Provided an opportunity to determine the level of understanding of this area of science in schoolchildren. |
| Year(s) Of Engagement Activity | 2015 |
| Description | Blog post for science website |
| Form Of Engagement Activity | Engagement focused website, blog or social media channel |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Interview with the scientific website Sparrho on the discovery and development of antibiotics |
| Year(s) Of Engagement Activity | 2015 |
| URL | http://blog.sparrho.com/post/121265105627/the-medicine-makers |
| Description | Dubrovnik Summer School |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Postgraduate students |
| Results and Impact | I provided a seminar and led multiple small group discussions as part of the Dubrovnik Summer School in Applied Molecular Microbiology. This was attended by 45 postgraduate and post-doctoral researchers from around the world to learn about concepts and methods in natural product biosynthesis. Following the summer school, I have been contacted by multiple attendees regarding various aspects of the work I discussed. |
| Year(s) Of Engagement Activity | 2018 |
| URL | https://www.jic.ac.uk/training-careers/summer-schools/applied-molecular-microbiology/ |
| Description | International Research Alliance for Antibiotic Discovery and Development (IRAADD) Workshop Meeting |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Attendance and presentation as part of the International Research Alliance for Antibiotic Discovery and Development (IRAADD) Workshop Meeting (Saarbruecken, Germany), which is a Europe-wide network of researchers and industry partners involved in antibiotic discovery and development. |
| Year(s) Of Engagement Activity | 2019 |
| Description | Interview with science writer (Kat Arney) about antibiotic discovery and combating antimicrobial resistance. |
| Form Of Engagement Activity | A press release, press conference or response to a media enquiry/interview |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Media (as a channel to the public) |
| Results and Impact | Interview with science writer (Kat Arney) about antibiotic discovery and combating antimicrobial resistance. In particular a focus on the re-purposing of old antibiotics for treating multi-drug resistant infections. This was in relation to an in-depth article she is preparing on AMR and antibiotic discovery, which was later published in the Daily Mail. |
| Year(s) Of Engagement Activity | 2018 |
| URL | https://www.dailymail.co.uk/health/article-5623417/Could-antibiotics-mens-beards-soil-weapons-battle... |
| Description | Invited talk at China-UK AMR Workshop |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | A UKRI-funded workshop held in Beijing to discuss ongoing and recent UK-China research projects on AMR (antimicrobial resistance), as well as future directions and prospects for this research area. |
| Year(s) Of Engagement Activity | 2019 |
| Description | Invited talk at Environmental Genomics and Advanced Microbiological Techniques Workshop |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Postgraduate students |
| Results and Impact | Presented a talk about using mass spectrometry-based metabolomics in microbiology to PhD students attending an Environmental Genomics and Advanced Microbiological Techniques Workshop at the University of East Anglia. I received multiple questions after the seminar. |
| Year(s) Of Engagement Activity | 2019 |
| Description | Invited talk at Helmholtz Institute for Pharmaceutical Research Saarland |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Postgraduate students |
| Results and Impact | Invited talk entitled "Deciphering the biosynthesis of peptidic natural products" given at the Helmholtz Institute for Pharmaceutical Research Saarland (HIPS), Germany |
| Year(s) Of Engagement Activity | 2016 |
| Description | Invited talk at Plant-Microbe Interactions Workshop |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Professional Practitioners |
| Results and Impact | Seminar provided on Streptomyces-Pseudomonas interactions at a Plant-Microbe Interactions Workshop organised at the John Innes Centre. |
| Year(s) Of Engagement Activity | 2019 |
| Description | Invited talk at the University of East Anglia (BIO Open Lecture) |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Postgraduate students |
| Results and Impact | Invited talk entitled "Deciphering the biosynthesis of peptide antibiotics" given at the University of East Anglia (BIO Open Lecture) |
| Year(s) Of Engagement Activity | 2016 |
| Description | Invited talk at the University of Ghent |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Postgraduate students |
| Results and Impact | Invited talk on natural product discovery at the University of Ghent (Belgium). |
| Year(s) Of Engagement Activity | 2019 |
| Description | Meeting with Science Museum about an exhibition on antibiotics |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Other audiences |
| Results and Impact | A meeting with Science Museum staff about content for a new exhibit on antimicrobial resistance. Information leaflets were prepared, along with information about the wide array of AMR research taking place across the Norwich Research Park. UEA later became formally involved in this "Superbugs" exhibition. |
| Year(s) Of Engagement Activity | 2016 |
| URL | https://www.sciencemuseum.org.uk/see-and-do/superbugs-fight-our-lives |
| Description | Presentation at Xiamen University (China) |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Postgraduate students |
| Results and Impact | Invited presentation given to faculty, post-docs and students at Xiamen University in China. |
| Year(s) Of Engagement Activity | 2019 |
| Description | Presentation to Bayer Agronomy team |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Industry/Business |
| Results and Impact | Presentation and discussion with the agronomy team at Bayer about biocontrol products |
| Year(s) Of Engagement Activity | 2019 |
| Description | School visit (INTO Norwich) |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Schools |
| Results and Impact | One hour presentation entitled "Looking for drugs made by bugs" presented to international students participating in the Newton Programme at INTO Norwich. Purpose was to communicate my research to A-level students interested in science. Following the presentation, the teacher reported: "The students have been very keen to discuss points you raised in class (we are studying antibiotics this week) and were excited to see the links between the knowledge they have gained through their AS studies and your research." |
| Year(s) Of Engagement Activity | 2014 |
| Description | Science Museum Superbugs Late Event |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Public/other audiences |
| Results and Impact | "Antibiotic Hunters" exhibit at a Science Museum Superbug Late Event. This was an adult-only event held in the evening at the Science Museum in London and attended by thousands of people. The exhibit was organised jointly between members of my research group and members of the Wilkinson group (also at JIC). |
| Year(s) Of Engagement Activity | 2018 |
| Description | Talk given at the 1st International RiPPS Conference |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Talk given on natural product discovery at the 1st International RiPPS Conference, held in Granada, Spain |
| Year(s) Of Engagement Activity | 2019 |
| URL | https://www.rippsconference.org/ |
| Description | Year 10 Science Camp Talk |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | A talk on natural product discovery to attendees of the year 10 Science Camp at the John Innes Centre. This was followed by questions on antibiotic and anticancer compound discovery. |
| Year(s) Of Engagement Activity | 2018 |
| URL | https://www.jic.ac.uk/training-careers/work-experience/year-10-science-camp/ |