The molecular basis of post-initiation regulation of RNA transcription

Lead Research Organisation: John Innes Centre
Department Name: Contracts Office


Post-initiation regulation of RNA transcription during the elongation phase by the alternative pathways of termination, pausing or elongation allows the cell or organism subtle control over the expression of genes that are constitutively expressed or produced in response to environmental signals. It is the kinetic competition between the rates of these alternative pathways that are available to the elongation complex at pause or termination sites, which are modulated by transcription cofactors, such as NasR, NusG, NusA or the boxA antitermination complex. This proposal aims to understand how these kinetic rates are altered by these transcription cofactors using a novel surface plasmon resonance (Biacore) assay to study two bacterial antitermination systems, the NasR and boxA antitermination complex regulation of the nasF and ribosomal RNA operons respectively.


10 25 50
Description Improved understanding of transcription of genes and novel usage of hi-tech technologies (Surface Plasmon Resonance).
Exploitation Route Applications of SPR to study transcription
Sectors Healthcare,Pharmaceuticals and Medical Biotechnology

Description The proposal to develop a high-throughput method to rapidly identify and characterise the binding affinity of transcription factor DNA binding sequences using Surface Plasmon Resonance (SPR) array technology has broad application for both microbial and plant gene regulation across many of the departments within the John Innes Centre. It is likely that future application of this technology will also benefit the general UK scientific community at large.
First Year Of Impact 2010
Sector Pharmaceuticals and Medical Biotechnology
Impact Types Policy & public services