Public health impact of long-term, low-level mixed element exposure in susceptible population strata

Lead Research Organisation: John Innes Centre
Department Name: Contracts Office

Abstract

At least one third of the world's population is thought to suffer from zinc deficiency. Dietary zinc deficiency is particularly prevalent in populations that rely extensively on cereals for calorific intake since the endosperm of cereal grains is poor in zinc.
We have identified and characterised a barley zinc transporter (HvMTP1) that is located at the vacuolar membrane and which is responsible for vacuolar zinc accumulation. The metal specificity determinants of HvMTP1 have been identified (a) by constructing chimeras between HvMTP1 (which transports both Zn and Co) and Arabidopsis MTP1 (which is selective for Zn); (b) through random mutagenesis followed by a yeast-based screen on different transition metals; (c) through site-directed mutagenesis. A five-residue domain in a cytosolically-located histidine-rich loop is critical in facilitating discrimination between Zn and Co, while residues in the third transmembrane domain are involved in encoding specificity for Zn over a number of other transition metals.
HvMTP1 is poorly expressed in endosperm, although in other regions of the seed (aleurone, endosperm) HvMTP1 transcript is abundant. This expression pattern correlates with the regions of cereal grains in which Zn is known to be accumulated. By expressing HvMTP1 on a D-hordein promoter - which is endosperm-specific - we are examining the hypothesis that the transporter plays a pivotal role in Zn accumulation in vivo. If this is indeed the case, modulation of MTP1 expression might provide a means to biofortify cereal grains with Zn.

Publications

10 25 50
 
Description Not applicable at this stage.
Exploitation Route N/A
Sectors Agriculture, Food and Drink,Environment

 
Description CEPAMS Symposium Shanghai 2016 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact CEPAMS Symposium Shanghai 2016 Friday 18th March
Editing Long Distance Transport and Storage to Optimize the Site of Nitrogen Assimilation in Drought and Salt-stress
Year(s) Of Engagement Activity 2016
 
Description International Symposium on Plant Membrane Biology, Annapolis MD, USA 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact International Symposium on Plant Membrane Biology, Annapolis MD, 2016
Thursday 9th June 2016 S16 Future Perspectives
Year(s) Of Engagement Activity 2016
 
Description Invited Seminar at SIPPE Shanghai Institute of Plant Physiology and Ecology 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact 4th November 2016 in Shanghai 15:00
Invited Seminar at SIPPE Shanghai Institute of Plant Physiology and Ecology
Year(s) Of Engagement Activity 2016
 
Description Invited seminar speaker Padua Italy (12th January 2016) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Presentation on the role of the plant lytic vacuole.
Title: Lytic Vacuole: Space-Filler, Garbage Bag, or Something More Interesting?
Year(s) Of Engagement Activity 2016
 
Description Invited seminar speaker Plant Sciences Univiersity of Oxford, UK (January 28 th 2016) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact Invited Departmental seminar speaker. Topic was the role of the lytic vacuole.
Title: The Plant Lytic Vacuole: Space-Filler, Garbage Bag, or Something More Interesting?
Year(s) Of Engagement Activity 2016
 
Description World Life Science Conference (Sustainable Agriculture Session), Beijing, 2016 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact World Life Science Conference (Sustainable Agriculture Session), Beijing, 2016
3rd November 2016, Session 48 Sustainable Agriculture - Membrane Transporters and Nutrition - from crops to people
Year(s) Of Engagement Activity 2016