Crop Quality

Lead Research Organisation: John Innes Centre
Department Name: UNLISTED

Abstract

Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.

Technical Summary

Recent progress in genomic and transcriptomic technologies for crop species provides new opportunities to apply our expertise directly to these species, in order to underpin improvements in crop quality. We have an outstanding track record in research on the nutritional importance of phenylpropanoids and on the composition of pea seeds. We also have expertise in understanding plant zinc and iron homeostasis and in starch synthesis and turnover. Across these research areas, we will use new knowledge of the genetic control of crop composition to develop suites of crop plants differing only in a single aspect of composition. These will be tested for health promoting, digestive and functional properties by researchers at the neighbouring Quadram Institute. The outcome will be a new, systematic understanding of bioavailability and nutritional importance of crop components, the effects of composition on food functionality and consumer acceptability, and means of breeding nutritionally superior crops. We will apply understanding of zinc and iron homeostasis to increase the iron and zinc content of cereal grains, and to exploit iron in pea seeds. We will use knowledge of the relationship between starch properties, starch synthesis and starch granule initiation to develop wheat lines containing starches that differ in resistance to digestion, and to discover how granules develop in wheat grains. We will make use of new pea germplasm to provide systematic understanding of important nutritional and quality traits in pea. We will develop new resources for studying the importance of dietary phenylpropanoids for human health, and discover genes underlying quality traits in citrus fruit.

Planned Impact

unavailable

Publications

10 25 50
 
Title Paper made from grass pea straw 
Description Paper made from grass pea straw by local artist and designer Caroline Hyde-Brown Norwich University of the Arts 
Type Of Art Artwork 
Year Produced 2021 
Impact Paper made from grass pea straw by local artist and designer Caroline Hyde-Brown Norwich University of the Arts: http://www.livingfield.co.uk/fibres/repurposing-grass-pea-as-an-embroidered-textile-and-handmade-paper/ 
URL http://www.livingfield.co.uk/fibres/repurposing-grass-pea-as-an-embroidered-textile-and-handmade-pap...
 
Description Key Findings 2017/2018:
Connorton et al. (Plant Physiology 174: 2434-2444, 2017. doi:10.1104/pp.17.00672) described a strategy to increase the iron content of wheat grain, where the iron is redistributed to the endosperm, the part of the grain that is milled to white flour. This fraction is relatively low in phyate, an inhibitor of iron uptake for human and animal nutrition, meaning that the iron is more bioavailable. We confirmed this by studies on human gut cells, in collaboration with the Norwich Medical School.

Menguer et al. (Plant Biotechnology Journal (in press). doi:10.1111/pbi.12749) demonstrated that endosperm Zn content can be enhanced through expression of a transporter responsible for vacuolar zinc accumulation in cereals. The demonstration of a redistribution of grain Zn from aleurone to endosperm provides the basis of a strategy for biofortification of cereal endosperm with Zn.

Kenzhebayeva et al. (Journal of Crop Improvement 31:209-228, 2017. doi:10.1080/15427528.2016.1276990) showed that there is capacity to biofortify wheat grain 4-fold with iron and zinc without negatively impacting on crop productivity.

Chen et al. (Journal of Experimental Botany 68:3007-3016, 2017. doi:10.1093/jxb/erx165) showed that a plasma membrane transporter belonging to the Nodulin 26-like intrinsic membrane protein (NIP) family plays a role in the uptake of the toxic metal arsenic by rice lateral roots.

Using the in silico wheat TILLING resource (exome capture and re-sequencing of hexaploid Cadenza mutants), we selected lines carrying null mutations in each homoeoallele of seven starch synthase and starch branching enzyme genes expected to influence starch structure and composition (GBSSI, SSI, SSIIa, SSIIIa, SBEI, SBEIIa, SBEIIb). We initiated a crossing program to generate single-, double-, and triple-null mutants for each gene using different combinations of mutant homoeoalleles. These null mutants were expected to exhibit distinct starch structural properties, to enable exploration of the role of each homoeologue in amylose and amylopectin synthesis and establish how this relates to starch digestibility.

Using the wheat TILLING population, the exome capture database and the wheat genome sequence, we selected lines with mutations in orthologues of three genes established to be important for starch granule initiation (SGI genes) in Arabidopsis. Mutations in both homoeoalleles of the genes in the tetraploid background Kronos were selected and the lines subjected to a crossing programme to generate null mutants. Initial characterisation of the three lines revealed that all have profoundly altered distribution of starch granule size and shape in the endosperm. This result suggested that the function of the SGI proteins is conserved between Arabidopsis and wheat, and provides unique material for 1) further discovery of the mechanism of granule number and shape determination in wheat and 2) evaluation of functional properties of interest to industry.

Rayner et al. (International Journal of Molecular Sciences 18:1205, 2017. doi:10.3390/ijms18061205) used high-throughput screening methods and an extensive germplasm resource to identify novel variation affecting seed composition and shape in pea. Seed shape variation was attributed to at least four genetic loci. A novel seed shape trait was associated with alterations to myoinositol metabolism, with broader implications for seed composition.

Watson et al. (Nature Plants 4:23-29, 2018. doi:10.1038/s41477-017-0083-8) described how 'Speed breeding' will accelerate the introgression of novel and improved traits into breeders' lines of pea and other crops. We showed how the generation time of pea could be reduced, using the genetic background of the seed protein mutant lines.

D'Amelia et al. (Plant, Cell & Environment, 2017. doi: 10.1111/pce.12966) showed that the expression of a MYB transcription factor, AN2, is induced by low temperatures in wild, cold-tolerant Solanum commersonii, and not in susceptible Solanum tuberosum, varieties. We propose that the duplication of R2R3 MYB genes resulted in sub-functionalization, whereby AN1 specialized in anthocyanin production and AN2 conserved the ability to respond to cold stress, inducing mainly the synthesis of hydroxycinnamic acid derivatives. These results contribute to understanding the evolutionary significance of gene duplication on phenolic compound regulation.

Kallam et al. (Current Biology 27:945-957, 2017. doi.org/10.1016/j.cub.2017.02.027) described how aromatic decoration (acylation) of anthocyanins leads to the formation of anthocyanic vacuolar inclusions (AVIs), solving a long-standing puzzle in anthocyanin biochemistry. No other compounds were found to be present in AVIs, and glycosylation of acylated anthocyanins was shown to reduce their propensity to form AVIs. We defined, for the first time, the chemical characteristics that influence the precipitation of anthocyanins, both in vivo and in vitro, and showed that precipitation is a function of the quinoidal forms of these pigments, in solution.

Scarano et al. (Frontiers in Nutrition 4:75, 2018. doi.org/10.3389/fnut.2017.00075) described the development of a tomato variety 'Bronze', enriched in three distinct classes of polyphenols: flavonols, anthocyanins, and stilbenoids. In healthy mice, diets supplemented with either anthocyanins and flavonols or stilbenoids promoted the growth of 'healthy bacteria' in the gut microbiota. In a mouse model, nutritional intervention with the bronze variety alleviated the symptoms of inflammatory bowel disease, probably through suppression of inflammation as well as through beneficial changes in the composition of gut microbiota.

Tomlinson et al. (Frontiers in Nutrition 4:61, 2017. doi.org/10.3389/fnut.2017.00061) investigated the anti-inflammatory effects of polyphenols, and showed that tomato extracts enriched in anthocyanins or flavonols have strong and specific inhibitory effects on a set of key pro-inflammatory cytokines and chemokines.

Zhao et al. (Molecular Plant 11:135-148, 2018. doi.org/10.1016/j.molp.2017.08.009) reported the isolation and characterization of two CYP450 enzymes, which function as the flavone 6-hydroxylase and flavone 8-hydroxylase, respectively, in the medicinal plant Scutellaria baicalensis Georgi. The resulting 4' deoxyflavones can induce apoptosis in a variety of cancer cell lines, with no effect on normal cells, and have many additional benefits for human health, such as anti-oxidant, antiviral, and liver-protective properties. This paper represents a significant output and highlight of the JIC-Chinese Academy of Sciences alliance.

Key Findings 2018/2019:

Objective 3.1: We showed that white flour fractions representing those obtained by industrial milling of a cisgenic high-iron wheat line (Connorton et al. 2017) have >3-fold higher iron content (Balk et al., 2018). An application for a field trial of the high-iron wheat line was submitted to Defra. This will enable us to see if the high-iron trait, so far studied in growth rooms and glasshouses, is also observed in field-grown plants. An additional key finding on iron in plant foods is that pea seeds accumulate the iron storage ferritin prior to a sharp increase in phytic acid concentration. Iron-loaded ferritin is already abundant in immature peas, such as frozen garden peas, and this has good bioavailability in vitro correlating with lower phytate levels (Moore et al., 2018).
We reviewed strategies for growing and breeding forage crops with improved nutritional status (Capstaff and Miller, 2018).

Objective 3.2: We generated three wheat mutants lacking different components of the starch granule initiation in Arabidopsis and identified profound changes in starch granules in grain endosperm. In two of the mutants, the size distribution of starch granules is altered - granules are much larger in one and smaller in the other mutant - with no apparent effect on grain starch content. Wheat starch with uniformly small granules has many potential industrial applications: we applied for IP protection on this discovery.
In related research funded by a responsive mode BBSRC grant, we demonstrated (jointly with the Designing Future Wheat ISP) that the mature weight of wheat grains in the field is not influenced by variation between cultivars in either the capacity for starch synthesis or the substrate supply during the period of grain filling (Fahy et al., 2018). This information is important for attempts to develop cultivars with altered starch properties.

Objective 3.3: We showed that deletion of the pea lectin gene does not negatively impact on nodulation (Rayner et al., 2018), an important finding in relation to the nutritional enhancement of seed products. We described the genetic basis for variation in several classes of seed metabolites in pea, including branched-chain amino acids (Ellis et al., 2018). Some of this variation may underpin one or more of the quantitative trait loci we identified which affect seed size (Moreau et al., 2018). In collaboration with QIB, we contributed to technological developments that facilitate the analysis of seed biopolymers (Perez-Moral et al., 2018). In collaborations with several groups at JIC, we contributed to 'speed breeding' experiments, where protocols for the rapid cycling of pea and other crops were made available (Ghosh et al., 2018), enabling the rapid transfer of key discoveries to breeders' lines.

Objective 3.4: We described (Butelli et al., 2018) how, in citrus, the production of anthocyanin pigments requires the activity of the transcriptional activator Ruby. Consequently, loss-of-function mutations in Ruby result in an anthocyaninless phenotype. Several citrus accessions, however, have lost the ability to produce these pigments despite the presence of wild-type Ruby alleles. Here the lack of anthocyanins in young leaves and flowers is also associated with a lack of proanthocyanidins in seeds and an extreme reduction in fruit acidity, defined as "acidless". We identified Noemi, encoding a basic helix-loop-helix (bHLH) transcription factor, where large deletions or insertions of retrotransposons are associated with acidless phenotypes across a number of species (Citron, limetta, sweet lime, lemon, and sweet orange). In two accessions of limetta, a change in the core promoter region of Noemi is associated with reduced expression and increased pH of juice, indicating that Noemi is a major determinant of fruit acidity.
We have discussed (Li et al. 2018) how specialized secondary metabolites serve not only to protect plants against abiotic and biotic challenges, but have also been used extensively by humans to combat diseases. We argue that metabolic engineering for producing secondary metabolites in plants may have distinct advantages over microbial production platforms. Metabolic engineering strategies include: introducing biosynthetic genes, using transcription factors, and improving metabolic flux. Recent progress in biotechnology contributes markedly to better engineering, such as the use of specific promoters and the deletion of competing branch pathways. We proposed that next-generation plant metabolic engineering will improve current engineering strategies for producing valuable metabolites in plants on industrial scales.
We reviewed (Martin, 2018) how the challenges of achieving global food security have become more demanding as scientists have realized that not only calorie content but also food composition and colonic microbial content impact our health and well-being, dramatically. The ways that the nutrients we consume affect our health are highly complex due to the diversity of foods, their digestibility and nutrient bioavailability, variation in individual gut microbiota, and the multi-fold mechanisms of action that nutrients have on our health. It has been accepted for more than 50 years that diets rich in plants, particularly fruit and vegetables, protect health, and yet such diets have declined, with lower fruit and vegetable content and much more processed foods, over the same period. These dietary shifts have had a marked impact on the incidence of chronic diseases. Greater support for research into the ways that plant-based foods impact health will be essential for changing dietary patterns to protect health and to achieve global nutritional security.

Key Findings 2019/2020:

Objective 3.1: The first field trial of the genetically modified high-iron wheat line was conducted in 2019, after a licence (3 years) was obtained from Defra. Growth of the transgenic plants was similar to non-modified controls, but iron in the white flour fraction was significantly increased. Two more field trials were planned for 2021/ 2022. Bioavailability studies using Caco-2 cell cultures were planned in collaboration with Prof Paul Sharp, King's College London (BBSRC-funded grant). RNAseq analysis of developing grains of the high-iron wheat lines identified a transcription factor as a target for non-transgenic biofortification. TILLING lines of this gene were crossed to stack null mutations in the tetraploid Kronos wheat.
Research into pea ferritin has focussed on developing a nutritional iron supplement (see Impact section), and the evaluation of pea genotypes for high ferritin will be carried out in year 4.
Rice mutants and transgenic lines with altered cytokinin levels were used to demonstrate a key role for the hormone in controlling plant zinc distribution, including grain accumulation (Gao et al., 2019). The expression of zinc uptake transporters and chelators for internal transport depended on plant cytokinin levels.

Objective 3.2: We generated five wheat mutants lacking different proteins involved in starch granule initiation, which were originally discovered in Arabidopsis. Three of the mutants had profound changes in the shape and size of starch granules in the endosperm. Specifically, one of the mutants produced the same amount of total starch as the wild type, but with a strong overall decrease in starch granule size and increase in granule number. This has many potential industrial applications (see Narrative Impact). In the other two mutants, starch granules were severely misformed, demonstrating that the proteins are essential for proper granule initiation and morphogenesis in wheat. However, for one of the genes, knocking down the expression, rather than eliminating the gene completely, led to a change in granule size distribution, favouring larger granules (Chia et al., 2020).
In collaborative work with QIB, using the in silico wheat TILLING resource (exome capture and re-sequencing of hexaploid Cadenza mutants) we have selected lines carrying null mutations in each homoeoallele of 5 starch synthase, 3 starch branching enzyme, and 1 debranching enzyme genes expected to influence starch structure and composition. We initiated a crossing program to generate single-, double-, and triple-null mutants for each gene using different combinations of mutant homoeoalleles. For seven of these genes, we have selected homozygous single, double and triple mutants. For two of the genes we have identified triple heterozygous individuals to produce segregating F2 populations for selection of single, double and triple mutants. We have completed an initial characterization of starch and grain properties of single, double and triple mutants for one of the starch synthase genes (starch content, amylose content, resistant starch content, protein content, starch chain length distribution, starch granule size distribution, starch granule morphology, germination index, grain hardness, grain weight, grain dimensions, physicochemical properties). We showed that loss of this starch synthase in triple mutants leads to more amylose and resistant starch. Our results indicate that, despite the higher levels of resistant starch, other effects on starch and grain properties may hamper the usefulness of the mutants in breeding applications.
In closely-related work funded by the BBSRC Synthetic Biology Centre OpenPlant, we have used Cas9 gene-editing technology in potato to severely reduce the expression of one or both of the two forms of starch-branching enzyme, an essential determinant of starch polymer structure and hence the shape and properties of starch granules. We were successful in generating lines of potatoes with strong alterations in starch properties, which were transgene-free (Tuncel et al., 2019). The altered starches are likely to have lower digestibility in the upper gut, and hence to pose less risk to individuals prone to diabetes and related illnesses.

Objective 3.3: We participated in a genome-wide association study (GWAS), which used 135 pea accessions from 23 different international breeding programs in multi-year, multi-location trials (Gali et al., 2019). Significant marker-trait associations were identified, following genotyping-by-sequencing. These included seed protein and starch concentration, providing potential for SNP marker-assisted selection towards rapid cultivar improvement, in conjunction with 'Speed Breeding'. In a collaborative study (Santos et al., 2019), the physicochemical functional properties of seeds from pea germplasm were examined. A collection of 105 pea accessions was analysed for physicochemical properties, pasting viscosity, and basic composition (protein, fibre, fat, and resistant starch). Phenotypic screens were shown to be poor indicators of functional properties. However, genetic screens of a subset of 32 lines showed a correlation between genotype and physicochemical properties, pasting viscosity, and basic compositional parameters (p < 0.05). We reviewed the potential for improving pulse crops as a source of protein, starch and micronutrients (Robinson et al., 2019); this was a response to an invitation to contribute to an evaluation of the broad dietary shifts required to mitigate anthropogenic climate change and promote human health, where pulse crops can make an important contribution.
We improved the genome sequence of grass pea (Lathyrus sativus) by PromethION Nanopore sequencing that allows more accurate assembly of repeat elements in long reads. Polishing this Redbean assembly with the assembly from Illumina Mate-pair reads now gives BUSCO scores of 89% complete, 82% single copy complete BUSCOs from different databases. The PromethION sequencing was undertaken in collaboration with Levi Yant (now at University of Nottingham). The improved assembly should greatly facilitate marker development for grass pea and resequencing of 384 core accessions to be undertaken in collaboration with ICARDA under a Templeton project for improvement and development of pre-breeding lines of grass pea and finger millet.

Objective 3.4: Butelli et al. (2018) described Noemi and its role in acidification in citrus. An iCASE PhD student has now identified a mutation in sweet orange affecting the gene Nicole, another component of the complex controlling transcription of genes required for acidification in citrus. The student is confirming target genes of the regulatory complex, that will allow us to build a model of how cells hyper-acidify.
Following up on reviews describing how many of the challenges of achieving global food security have become more demanding as scientists realize that not only calorie content but also food composition and colonic microbial content impact our health and well-being, we supported and won a PhD project student funded by Breast Cancer UK to investigate the ways that polyphenols in the diet impact the microbiota and, in turn, the inception and progression of breast cancer (see Narrative Impact).
We promoted greater support for research into the ways that plant-based foods impact health by drafting a PhD programme, entitled EDESIA: Plants, food and health, from crop to clinic, awarded by the Wellcome Foundation (see Narrative Impact).

Key Findings 2020/2021:

Objective 3.1: To find new gene candidates for biofortification of wheat with iron and zinc, we have carried out analysis on TILLING mutants. Specifically, we have combined mutations in wheat homologues of the Iron Transporter 1 (IRT1), which is well characterized in Arabidopsis. We are studying mutations in the transcription factor IRO3, which was found to be deregulated in RNA-seq data of the high-iron wheat line.
The dgl mutation in pea, which causes iron hyper-accumulation, has been crossed into five previously identified lines with elevated ferritin. RNA-seq analysis on the dgl line showed that 4 ferritin genes are >10-fold upregulated as a consequence of the dgl mutation itself, and it will be interesting to see if this is further enhanced in the F2 generation of the crosses. In addition, we have obtained a cryo-EM structure of pea ferritin, isolated from pea seeds. This is the first structure of a native form of plant ferritin, to our knowledge, as other structures are from recombinantly expressed proteins.

Objective 3.2: We are using genetic and biochemical approaches to further understand starch granule initiation in wheat. Whereas Arabidopsis mutants deficient in any of the key components of granule initiation (SS4, PTST2 and MRC) have reduced numbers of starch granules in leaf chloroplasts, wheat mutants lacking these proteins produced a variety of different phenotypes in the endosperm and, in some cases, had increased numbers of granules per amyloplast (Hawkins et al., 2021). This suggests differences in the mechanism of granule initiation across organs/species. We are investigating genetic interactions between these granule initiation components in wheat by examining the effect of multiple mutant combinations on granule shape, size and number in the endosperm. We have also identified several uncharacterised plastid-localised proteins that interact with the granule initiation proteins. Wheat TILLING mutants lacking these new candidate proteins are currently being isolated. We expect these approaches will deepen understanding of starch granule initiation in wheat endosperm, as well as create more novel variation in starch granule shape and size. We reviewed the potential for adopting genetic approaches to manipulate granule initiation and achieve targeted starch modification in plants (Chen et al., 2021).
In addition to exploring starch granule initiation in crop species, we are taking a broad, comparative approach that exploits starch granule diversity in wild grass species. Granule initiation patterns in seeds vary considerably in grass species within the Pooideae (which includes wheat, oats and barley). Individual species exhibit specific patterns of granule initiation, including initiation per plastid of single granules, or multiple granules simultaneously, or single granules followed at a later stage by initiation of multiple granules. By comparing transcriptome patterns through the phase of granule initiation in species that differ in initiation patterns, we expect to identify key genes or groups of genes associated with each of these patterns and thus elucidate how the patterns arise.

Objective 3.3: We reviewed the basis for the genetic improvement of health- and nutrition-related traits in pea (Robinson and Domoney, 2020). We contributed to the research and publication which showed that a natural mutation in pea, impacting on starch and protein quality, can improve glucose homeostasis in humans (Petropoulou et al., 2020). We generated a new and superior high-quality reference genome sequence for a pea (P. sativum) accession (JI 2822), which now aligns well to genetic maps. The new assembly from PacBio HiFi sequences has resulted in a total genome length of 3.8 Gb, comprising 2,815 contigs (N50 = 4,162,731 bp) and has markedly improved the order and physical distances between genes compared to our earlier JI 2822 assembly and to that of the published cv. Cameor v1a assembly. The new reference is supporting several gene discovery projects, including the definition and mapping of deletions impacting on seed protein quality and, together with transcriptomic resources, is enabling the molecular genetic basis for a chlorophyll metabolism mutation to be elucidated. It is also being used in a collaborative genomics project with AGIS, China.
We published the draft genome sequence of grass pea as a preprint so that data are available for analysis by others to join a consortium publication. Grass pea is a resilient diploid (2n=14) legume closely related to pea (Pisum sativum). We determined the genome size of the sequenced European accession (LS007) as 6.3 Gbp. We generated two assemblies of this genome: i) EIv1 using Illumina PCR-free paired-end sequencing and assembly followed by long-mate-pair scaffolding and ii) Rbp using Oxford Nanopore Technologies long-read sequencing and assembly followed by polishing with Illumina paired-end data. EIv1 has a total length of 8.12 Gbp (including 1.9 billion Ns) and scaffold N50 59.7 kbp. Annotation has identified 33,819 high confidence genes in the assembly. Rbp has a total length of 6.2 Gbp (with no Ns) and a contig N50 of 155.7 kbp. Gene space assessment using the eukaryote BUSCO database showed completeness scores of 82.8 % and 89.8%, respectively.
We also developed two novel methods to assay ODAP, the toxin in grass pea. The first, a version of a widely used spectrophotometric assay, was modified for increased throughput, and permits rapid screening of large populations of germplasm for low toxin lines and the second is a novel, mass spectrometric procedure to detect very small quantities of ODAP for research purposes and characterisation of new varieties. In addition, we have developed a novel liquid chromatography mass spectrometry (LCMS)-based method for ß-L-ODAP-quantification. This method utilises an internal standard (di-13C-labelled ß-L-ODAP), allowing accurate quantification of ß-L-ODAP in grass pea tissue samples. The two methods have been compared; the spectrophotometric assay lacks sensitivity and detected ODAP-like absorbance in chickpea and pea but can be adapted for high throughput screens facilitating phenotyping of low ß-L-ODAP variation. The LCMS method did not detect any ß-L-ODAP in chickpea or pea. The LCMS method has been used to quantify ß-L-ODAP accurately in different tissues of grass pea.

Key Findings 2022/2023:
Objective 3.1, Iron and zinc content (Balk, Miller, Sanders): Transgenic wheat lines overexpressing two genes, the Vacuolar Iron Transporter 2 (VIT2) from wheat and Nicotianamine Synthase (NAS2) from rice, were shown to (i) accumulate more than double the amount of iron in the grain endosperm and white flour fractions; (ii) accumulate two-fold more zinc in wholemeal flour and (iii) three-fold more nicotianamine, an enhancer of mineral solubility in human diets (Harrington et al. 2023). Three independent lines and control wheat were subjected to the first year of a three-year field trial period (Consent 21_R52_01, available on www. gov.co.uk; consent holder is Cristobal Uauy of the DFW ISP, with Balk as Co-I). Having identified a mutation causing iron accumulation in pea, up to 30-fold in leaves and up to 4-fold in seed, Balk has filed a patent for engineering this and similar mutations in the same genes in crops (LU502457, filing date: 5/7/2022).

Objective 3.2, Starch content and properties (Hazard, Smith, Seung): We discovered that the starch phosphorylase, PHS1, is required for the normal initiation of B-type granules in wheat. Mutants lacking PHS1 have fewer, larger B-type granules compared to the wild type, but normal A-type granules. This trait is valuable for milling and brewing, so we have filed a patent for using PHS1 as a gene target for starch modification in wheat and barley (LU502613, filing date: 1/8/2022). To assess the feasibility of commercialising our wheat mutants with modified starch granule traits, we investigated the broader impact of the mutations on growth and physiology, comparing mutants in the same granule initiation gene across three different grass species (barley, wheat and Brachypodium). In addition to altering starch granules in the endosperm, the mutations affected starch metabolism in leaves of all three species. However, we observed no negative effect on growth in barley and wheat, likely due to the reliance of these plants on sucrose as the major storage compound in leaves (Watson-Lazowski et al. 2022).
We have developed a panel of wheat TILLING mutants for key genes in amylopectin biosynthesis: SSI, SSIIa, SSIIIa, SBEIIa, ISA1. Initial characterisation has revealed impacts on amylose content, resistant starch and starch granule morphology. Analysis of the ssIIIa genotypes showed the triple-null mutants had more resistant starch (6.0% vs. 2.9% in wild-type sibling controls) and greater levels of non-starch polysaccharides. Our analyses also revealed interesting gene dosage effects which could be used for fine-tuning starch properties; both double- and triple-null ssIIIa mutants differed from the wildtype in starch structure and composition including fewer long-amylopectin chains and more amylose (Fahy et al. 2022).
Objective 3.3, Seed protein content and properties (Domoney, Arora, Balk, Miller): Alongside research carried out within the Pulse Crop Genetic Improvement Network (PCGIN), we have generated combinations of up to three mutations which abolish the synthesis of proteins with low nutritional value, without affecting total protein concentration in pea seeds. In collaboration with CSIC, Spain, trials of a single mutation and a triple combination were carried out, using a standardised INFOGEST in vitro system. We have shown that there is a significant positive impact of the mutations on protein digestibility (Olias et al. Food Research International, in press). Given that seed protein concentration appears to be negatively associated with starch accumulation, we have studied a suite of mutations affecting the amount and type of starch in pea seeds. Associations between the nature of the mutation, its predicted impact on protein structure, quantity of starch and its chain length distribution were revealed (Moreau et al. 2022).
Objective 3.4, Health-promoting plant metabolites (Martin): We engineered the accumulation of provitamin D3 in tomato by genome editing, modifying a duplicated section of phytosterol biosynthesis in Solanaceous plants, to provide a biofortified food with the added possibility of supplement production from waste material (Li et al. 2022 Nature Plants).
Exploitation Route Our publications show that our findings are relevant to ongoing academic research. Individual research teams have relayed their results to external stakeholders through a number of meetings, for example, the Pulse Crop Genetic Improvement Network stakeholder forum and many individual meetings involving industry. We interact widely with industry and society, enabled by the Business Development, Knowledge Exchange and Commercialisation, and Communications teams, as well as through personal contacts with stakeholders and organisers of events, for example, science cafe and New Scientist Live discussion fora.
Sectors Agriculture

Food and Drink

Environment

Healthcare

 
Description Objective 3.1: In 2018, the development of the high-iron wheat line was presented at a meeting of the National Association of British and Irish Millers (NABIM) in London, at the Royal Norfolk Show, and at a gathering of the heads of Commonwealth at the Royal Society. Our findings of the bioavailability of iron in garden pea has been communicated to stakeholders such as PGRO and Birds Eye. The results were also presented at the November 2018 stakeholder meeting of the UK Pulse Crop Genetic Improvement Network. We contributed a review on iron biofortification of crops to a special issue of Plant & Cell Physiology, linked to the 19th International Symposium of Iron Nutrition in Plants which was held in Taiwan (Connorton & Balk, 2019). Our work on high-iron wheat featured on the BBC Breakfast show and was presented in 30 min talks at the Annual Meeting of the German Society of Plant Nutrition (Berlin) and the 13th European Nutrition Conference (Dublin). We received £60,000 funding from an industrial partner to upscale the extraction of ferritin from pea, which will be tested as an iron supplement in a small-scale clinical trial. The Balk group developed a good working relation with staff from the Clinical Research Facility at QIB, by carrying out food-grade extraction of pea ferritin using this facility over the course of 4 weeks. Interaction with the local community as well as an advert in the national press (Times) to carry out GM field trials of high iron, high zinc wheat at the JIC field station in Bawburgh. Objective 3.2: The ability to make genetic alterations to the shape and size of starch granules in the endosperm, without impacting on total starch content, has many potential industrial applications, and we applied for IP protection on this discovery. Objective 3.3: Funding was obtained in 2019 for an independent fellowship from the John Innes Foundation (the Ben Gill Translational Fellowship in pea genetics and genomics). Funding was secured for the Templeton Project to establish: A molecular breeding platform for rapid varietal development of grass pea (Lathyrus sativus) to unlock its potential as healthy food for malnourished people of South Asia and Sub-Saharan Africa (collaboration between The Crop Trust, ICARDA, and the James Hutton Institute). In 2020, an appointment was made to the Ben Gill Translational Fellowship in pea genetics and genomics, as a tenure-track JIC Group Leader. Funding has been secured from Defra to investigate the genetics of nutritional traits in pea, drawing on new genomic resources. The publication by Petropoulou et al. (2020) was the top performing media story of 2020 at JIC, leading to extensive newspaper, radio and TV (BBC, Channel 4) coverage. Dr Peter Emmrich secured a position as a John Innes Foundation Fellow in the Norwich Institute of Sustainable Development (January 2021- ). His position allows him to move the work on grass pea forward for translational and adoption activities on the low ODAP grass pea varieties he has developed in co-operation with the research on developing improved varieties at JIC. Dr Arora has secured funding from industrial partner for CASE PhD studentship on pea genomics and disease resistance and was involved in many outreach activities. Objective 3.4: Work by Martin and co-workers developed new collaborations, notably with Kew Gardens in the area of exploring traditional Chinese medicinal plants, and most recently with UEA, QIB and EI through a new PhD programme, entitled EDESIA: Plants food and health, from crop to clinic. This programme was awarded by the Wellcome Foundation, it will fund 5 students per annum over 5 years and recruited its first cohort of students in 2020. This will promote further collaborations into how phytonutrients promote health and protect against chronic diseases. Together with the award from Breast Cancer UK (BRUK) for a PhD project studentship (see Key Findings), these programmes provide valuable uplift to MfN. The BRUK student is supervised by Stephen Robinson at QIB, and we supply the polyphenol-enriched tomatoes for studies on xeno-grafted mice and advice through co-supervision. Impact narrative 2022/23: Objective 3.1: Members from the Balk group attended an information event with the local community surrounding the GM field trials of high iron, high zinc wheat at the JIC field station in Bawburgh. A short-term, IDG-funded position in the Balk group enabled a female researcher to return to a career in STEM after a parental career break. The person was subsequently recruited to a permanent post in industry. Balk also carried out mineral and phytic acid analyses for a company growing and selling frozen peas. Objective 3.2: Seung delivered five presentations to different industry stakeholder groups on the potential of new genetic targets to modify starch properties in wheat. This led to a new collaboration with a major breeding company, who are now bulking some of the wheat lines generated in Obj.3.2 for functional testing, including those related to the PHS1 technology above. We also started a collaboration with CIMMYT to transfer the most promising starch traits into a CIMMYT elite variety, Reedling. Seung also won industry funding from a major potato breeding company to test the relevance of the findings in wheat in potato, which led to a collaborative BBSRC IPA award (BB/X001520/1). Objective 3.3 A postdoctoral researcher co-supervised by Domoney and Balk gave three different presentations on new genetic variation for nutritional traits in pea to stakeholders in industry. A Farming Innovation Pathways grant has been secured by Domoney and co-workers with industry to advance the triple mutant combination generated in an elite genetic background of pea. Arora has secured funding for a CASE PhD studentship to identify sustainable sources for pea downy mildew resistance. The studentship is 50% funded by two industrial partners (KWS and Limagrain). Arora was also involved in several outreach activities e.g., industrial visits, presenting at scientific conferences and stakeholder meetings. Objective 3.4 A patent filing on the genetic technology to produce provitamin D3 in plants is attracting considerable commercial interest.
Sector Agriculture, Food and Drink,Environment,Healthcare,Pharmaceuticals and Medical Biotechnology
Impact Types Societal

Economic

Policy & public services

 
Description APPG attendance
Geographic Reach Multiple continents/international 
Policy Influence Type Participation in a guidance/advisory committee
Impact The policy brief to improve the nutritional quality of staple foods through biofortification is starting to have a huge impact globally. Asia and Africa are the main benefactors, although the impact differs from country to country. An important policy change is that biofortification could include genetic engineering, and not just breeding.
 
Description Elucidating the spatial and temporal control of granule initiation in wheat
Amount £468,825 (GBP)
Funding ID BB/W015935/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 07/2022 
End 08/2025
 
Description Newton Bhabha PhD Placement Programme
Amount £10,000 (GBP)
Organisation British Council 
Sector Charity/Non Profit
Country United Kingdom
Start 03/2022 
End 08/2022
 
Description Unlocking the Potential of Grasspea for Resilient Agriculture in Drought-prone Environments (UPGRADE)
Amount £1,246,884 (GBP)
Funding ID BB/R020604/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 06/2018 
End 06/2022
 
Description Unravelling novel mechanisms of starch granule biogenesis in potato
Amount £386,236 (GBP)
Funding ID BB/X001520/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 12/2022 
End 11/2025
 
Title Resources for Grass pea genomics and improvement through breeding 
Description GLDC diversity panel deposited in GRU Norwich together with sequencing data for EcoTILLING by sequencing. Also available through Germinate (germinate@hutton.ac.uk or https://ics.hutton.ac.uk/cwr/grasspea/#/home). Suitable for genomic selection of improvement traits in grass peaand of interest in particular to DAC countries Ethiopia, Bangladesh and India. 3 x RIL populations of 280 (LSWT007 x LSWT011), 220 (LSWT007 x Mahateora) and 87 (LSWT011 x Mahateora. 30 RILS of LSWT007 x LSWT011 resequenced. Lines deposited in GRU Norwich. Suitable for mapping of improvement traits in grass pea and of interest in particular to DAC countries Ethiopia, Bangladesh and India. 3000 M2 EMS mutagenized grass pea lines (LSWT007) available for TILLING by sequencing deposited in GRU Norwich. Suitable for reverse genetic development of improvement traits in grass pea and of interest in particular to DAC countries Ethiopia, Bangladesh and India. Protocol for grass pea transformation and gene editing manuscript in preparation. Suitable for engineering improvement traits in grass pea and of interest in particular to DAC countries Ethiopia, Bangladesh and India. Fourteen new low ODAP mutants of grass pea developed using EMS mutagenesis. Suitable for immediate deployment in breeding programmes for grass pea improvement and of interest in particular to DAC countries Ethiopia, Bangladesh and India. 
Type Of Material Biological samples 
Year Produced 2022 
Provided To Others? No  
Impact Not yet available 
 
Title Draft genome sequence assembly and annotation of grass pea LS007 
Description LS007 genomic PromethION and Illumina sequencing data have been deposited in the European Nucleotide Archive under accession PRJEB33571. Illumina RNAseq and HiC raw data have been deposited in the NCBI Gene Expression Omnibus under accession GSE223956. The genome assembly and annotations have been deposited on Zenodo [https://zenodo.org/record/7390878]. 
Type Of Material Database/Collection of data 
Year Produced 2023 
Provided To Others? Yes  
Impact none yet 
URL https://www.ebi.ac.uk/ena/
 
Title Germinate Grass pea database 
Description Germinate Grass pea database (germinate@hutton.ac.uk or https://ics.hutton.ac.uk/cwr/grasspea/#/home) 
Type Of Material Database/Collection of data 
Year Produced 2023 
Provided To Others? Yes  
Impact Genomic data for grass pea diversity panel for GWAS and mapping of improvement traits 
URL https://ics.hutton.ac.uk/get-germinate,
 
Description Analysis of potatoes subjected to Cas9-mediated mutagenesis of SBE genes in potato 
Organisation Quadram Institute Bioscience
Department Food & Health Programme
Country United Kingdom 
Sector Academic/University 
PI Contribution We partnered with colleagues in the Quadram Institute Bioscience to conduct analyses of starch structure in potato tubers generated at JIC in which starch branching enzymes genes were mutated by introduction of the Cas9 gene and sgRNAs targetting both sets of SBE homoeoalleles.
Collaborator Contribution QIB used state-of-the-art HPLC-SEC and 1H-NMR tools to examine the chain-length distributions and the ratio of alpha 1,4 to alpha 1,6 linkages in starch from wild-type and mutated potatoes in this analysis
Impact Manuscript submitted for publication to a peer-reviewed journal. The work made use of biophysical techniques for analysis of polymer structure, synthetic biology, and plant biochemistry and molecular biology
Start Year 2018
 
Description Meeting with representatives from The British Association of Green Crop Driers Ltd (BAGCD) 
Organisation British Association of Green Crop Driers
Country United Kingdom 
Sector Public 
PI Contribution meeting
Collaborator Contribution meeting
Impact n/a
Start Year 2015
 
Description Templeton Funding 
Organisation International Center for Agricultural Research in the Dry Areas
Country Syrian Arab Republic 
Sector Charity/Non Profit 
PI Contribution We are contributing the grass pea genome sequence plus resequencing of 384 grass pea accessions to a large project on improvement of grass pea and finger millet co-ordinated by ICARDA with partner James Hutton Institute for Germinate_3 database for phenotypes. This is funded by The Crop Trust by subcontract from ICARDA.
Collaborator Contribution We are contributing the first reference grass pea genome sequence plus resequencing of 384 grass pea accessions
Impact None yet
Start Year 2019
 
Title METHODS FOR ALTERING STARCH GRANULE PROFILE 
Description The invention relates to methods for altering the size distribution of starch granules in starch storage organs. Also described are genetically altered plants characterised by the above phenotype as well as methods of producing such plants. 
IP Reference WO2020065331 
Protection Patent application published
Year Protection Granted 2020
Licensed No
Impact While we have not licenced the technology yet, the filing of this patent has stimulated new discussions with cereal breeders, growers and processors. We have also engaged in new collaborative work with a food company that is not focused on cereals, to explore whether the technology can be adapted to other crops. This may lead to adoption of the technology in these other crops, and subsequent licencing.
 
Title Methods to increase iron content in plants 
Description The invention relates to genetically altered plants, parts thereof or plant cells, where the plants, parts thereof or plant cells are characterised by increased levels of iron. Also described are methods of increasing the levels of iron in a plant, particularly a crop plant as well as food compositions and nutritional supplements obtained from the plants or parts thereof. 
IP Reference LU502457 
Protection Patent / Patent application
Year Protection Granted 2022
Licensed No
Impact Too recent to say.
 
Company Name Norfolk Plant Sciences 
Description Norfolk Plant Sciences develops a genetically modified tomato, containing an anthocyanin biosynthesis gene from snapdragons, resulting in a purple fleshed tomato with a higher antioxidant content. 
Year Established 2007 
Impact Three granted patents: Luo, J., Butelli, E., Jones, J., Tomlinson, L. and Martin, C.R., Norfolk Plant Sciences Limited, 2017. Methods and compositions for modifying plant flavonoid composition and disease resistance. U.S. Patent 9,580,725. Martin, C. and Butelli, E., Norfolk Plant Sciences, Ltd., 2017. Methods for increasing the anthocyanin content of citrus fruit. U.S. Patent 9,574,202. Luo, J., Butelli, E., Jones, J., Tomlinson, L. and Martin, C.R., Norfolk Plant Sciences Limited, 2014. Method for modifying anthocyanin expression in solanaceous plants. U.S. Patent 8,802,925.
Website http://www.norfolkplantsciences.com
 
Description 'Pulse Research Update' - Press Visit to John Innes Centre and the Earlham Institute 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Media (as a channel to the public)
Results and Impact 'Pulse Research Update' - Press Visit to John Innes Centre and the Earlham Institute
Year(s) Of Engagement Activity 2018
 
Description 'Pulse Research Update' at John Innes Centre - PGRO & press visit 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Media (as a channel to the public)
Results and Impact 'Pulse Research Update' at John Innes Centre - PGRO & press visit
Year(s) Of Engagement Activity 2018
 
Description 1st workshop meeting of the MRC Global Nutrition and Health Health Outcomes in Undernutrition - The Role of Nutrients, Gut Dysfunction and the Gut Microbiome (HUNGer) 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact Attended the 1st workshop meeting of the MRC Global Nutrition and Health: Health Outcomes in Undernutrition - The Role of Nutrients, Gut Dysfunction and the Gut Microbiome (HUNGer)
Year(s) Of Engagement Activity 2018
 
Description Africa summer school 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact 20 postgraduate students from all over East and Central Africa attended a 2-week training course on crop improvement. I contributed lectures and a practical to study the iron content of cereal grains. Outcomes: (I) the students learned experimental techniques that are useful for their PhD research; (ii) One of the students is visiting our institute in the UK to carry out specific experiments for his PhD (January - March 2019)
Year(s) Of Engagement Activity 2017
URL http://nm-aist.ac.tz/documents/document-gallery/CREATES%20Summer%20School%20Feb.%2010,%202017.pdf
 
Description AgriGenomics, BOKU seminar 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Talk to postgraduate students and faculty at BOKU for improving plant health.
Year(s) Of Engagement Activity 2021
 
Description Attendance and presentation at Sensors in Food and Agriculture 2018 meeting 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Field testing and the research underpinning the soil sensor development was reported at the Sensors in Food and Agriculture 2018 meeting. At this meeting an industrial partner was identified who has relevant expertise to commercially develop the soil sensor technology.
Year(s) Of Engagement Activity 2018
URL http://www.rsc.org/events/detail/33486/sensors-in-food-and-agriculture-2018
 
Description BBC TV Countryfile Spring Diaries first broadcast by BBC1 on 28.05.18. Series 3, episode 1 0f 5 
Form Of Engagement Activity A broadcast e.g. TV/radio/film/podcast (other than news/press)
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Broadcast: Cathie Martin BBC TV Countryfile Spring Diaries first broadcast by BBC1 on Monday 28 May 2018 at 9:00. Series 3, episode 1 of 5. Incredible Edibles. With the world population predicted to grow by 35 per cent over the next 25 years, our food industry is under more pressure than ever before to come up with nutritional solutions that will feed us all. Margherita Taylor is in Norwich on the trail of some of the foods destined to land on our plates in the near future. At the John Innes Centre, trail-blazing scientists are working on these incredible edibles, among them purple tomatoes - specially developed to be super disease-busters. Professor Cathy Martin lets Margherita into a few secrets about just what it is that makes purple fruit and vegetables so life-enhancing. But will we want to give tomatoes that have been scientifically enhanced the thumbs up or the thumbs down? Shoppers at Norwich market - one of the oldest in the UK - give their verdict.
Year(s) Of Engagement Activity 2018
 
Description BBSRC Meeting: Identification of strategies to secure funding for excellent science and for delivery of requirements of industry 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Policymakers/politicians
Results and Impact BBSRC Meeting: Identification of strategies to secure funding for excellent science and for delivery of requirements of industry
Year(s) Of Engagement Activity 2018
 
Description Broadsheet advert for GM trials 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Half page advert in the Times (national newspaper) to announce our intention to carry out a GM field trial. This is part of the Defra-led application procedure to obtain a licence for GM field trials.
Year(s) Of Engagement Activity 2022
 
Description Celebrating 50 years of John Innes research in Norwich at The Cut Science Cafe in Halesworth, Suffolk 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Celebrating 50 years of John Innes research in Norwich at The Cut Science Cafe in Halesworth, Suffolk
Year(s) Of Engagement Activity 2018
 
Description Cereals 2021 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Other audiences
Results and Impact Talk at the Cereals 2021 event. The talk initiated discussion with farmers and industrial representatives in the audience about future of plant based proteins.
Year(s) Of Engagement Activity 2021
 
Description Chaired inaugural meeting of EPSO working group on Nutritional Security 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Chaired inaugural meeting of EPSO working group on Nutritional Security
Year(s) Of Engagement Activity 2018
 
Description Discussion meeting on BBSRC-LINK Pulse Crop Proposal 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Other audiences
Results and Impact Discussion meeting on BBSRC-LINK Pulse Crop Proposal
Year(s) Of Engagement Activity 2019
 
Description EPSO/FESPB Joint Congress 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact I was chair of the scientific advisory committee for this biennial meeting held in Copenhagen in 2018.
Year(s) Of Engagement Activity 2018
 
Description Exhibition at the Norfolk Farming Conference 2017 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Industry/Business
Results and Impact Exhibition at the Norfolk Farming Conference 2017, JIC Conference Centre, Norwich, Norfolk, UK
Year(s) Of Engagement Activity 2017
 
Description Exhibition at the Norwich Science Festival 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Pea TimeVine display at the Norwich Science Festival. Part of the '50 years of John Innes Centre Research in Norwich' display at the Norwich Forum.
Year(s) Of Engagement Activity 2017
 
Description Fascination in Plants Day - Stall about starch research 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact This event was run by the institute to promote plant sciences to the general public. We designed and ran a stall to demonstrate open questions in starch research, and how addressing them can improve crop quality and human health. The stall was interactive, and we answered many questions from the audience.
Year(s) Of Engagement Activity 2022
 
Description First Defra Research Advisory Group meeting 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Policymakers/politicians
Results and Impact Meeting with Defra and AHDB representatives, along with Research Advisory Group members and Genetic Improvement Network leads.
Year(s) Of Engagement Activity 2018
 
Description Food Hack 2020 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Other audiences
Results and Impact N/A
Year(s) Of Engagement Activity 2020
 
Description Food Innovation and Health and Metabolic Biology mini-symposium 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Postgraduate students
Results and Impact Food Innovation and Health and Metabolic Biology mini-symposium. A joint-ISP networking event for QI and JIC. For JIC - emphasis mainly (though not exclusively) on Theme 3 of Molecules from Nature ISP. Organised by Metabolic Biology Staff as an addition to the Metabolic Biology seminar series. Talks were by QI and JIC students.
Year(s) Of Engagement Activity 2019
 
Description Fourth Defra Research Advisory Group meeting 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Policymakers/politicians
Results and Impact Meeting with Defra and AHDB representatives, along with Research Advisory Group members and Genetic Improvement Network leads.
Year(s) Of Engagement Activity 2020
 
Description Genetic improvement of pea to replace soyabean in the diets of poultry and monogastric livestock (PeaGen) Conference Call 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Other audiences
Results and Impact Genetic improvement of pea to replace soyabean in the diets of poultry and monogastric livestock (PeaGen) Conference Call with project collaborators
Year(s) Of Engagement Activity 2018
 
Description Genetic improvement of pea to replace soyabean in the diets of poultry and monogastric livestock (PeaGen) project meeting 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Other audiences
Results and Impact Genetic improvement of pea to replace soyabean in the diets of poultry and monogastric livestock (PeaGen) project meeting
Year(s) Of Engagement Activity 2018
 
Description Genetics Society centenary celebration 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Genetics Society centenary celebration held at the John Innes Centre. Open day at JIC including a tour of the site and field station plots. Displays included pea time vine, pea tree. GRU display, grass peas display and fortified wheat display.
Year(s) Of Engagement Activity 2019
 
Description H2020 TomGEM Project meeting 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Second Annual Meeting of the TomGEM H2020 Collaborative project
Year(s) Of Engagement Activity 2018
 
Description HUNGer consortium meeting 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Other audiences
Results and Impact meeting with HUNGer consortium members
Year(s) Of Engagement Activity 2018
 
Description Health by stealth. In: JIC Advances volume 30, Winter 2018/19. Peas; past and present, pp. 3-5 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Other audiences
Results and Impact Health by stealth. In: JIC Advances volume 30, Winter 2018/19. Peas; past and present, pp. 3-5
Year(s) Of Engagement Activity 2018
 
Description I was keynote speaker at the Chenshan Meeting on specialised metabolism in plants 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I was keynote speaker at the Chenshan Meeting on specialised metabolism in plants
Year(s) Of Engagement Activity 2018
 
Description I was keynote speaker at the Hainan first National Meeting on specialised Metabolomics 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact I was a keynote speaker at the Hainan Meeting on metabolomics
Year(s) Of Engagement Activity 2018
 
Description Information evening on GM field trials, 23 Feb 2022 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact The JIC communications office had distributed flyers to all houses in the village of Bawburgh to invite them to an information evening about the planned GM field trials: what crop(s) will be grown, what genetic modification has been done, why it is important to do these experiments.
Year(s) Of Engagement Activity 2022
 
Description Institute Open Day - Breeders Day 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Industry/Business
Results and Impact Tour of JIC caged pea plot area at Breeders' Day 2016.
Year(s) Of Engagement Activity 2017
 
Description Institute Open Day - Breeders' Day 2018 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Tour of JIC caged pea plot area at Breeders' Day 2018.
Year(s) Of Engagement Activity 2018
 
Description Institute Open Day - Breeders' Day 2019 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Tour of JIC caged pea plot area at Breeders' Day 2019.
Year(s) Of Engagement Activity 2019
 
Description Institute Open Day - JIC Open Day 2017 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact JIC Open Day 2017: Pea TimeVine and displays based around the research from the Domoney Lab
Year(s) Of Engagement Activity 2017
URL https://storify.com/JIC_Comms/open-day-2017
 
Description Invitation from Agrii to Food Supply Innovation Event at Throws Farm Technology Centre, Dunmow, Essex 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Invitation from Agrii to Food Supply Innovation Event at Throws Farm Technology Centre, Dunmow, Essex
Year(s) Of Engagement Activity 2018
 
Description Invited Seminar at Bristol University 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact Invited seminar speaker at Bristol University - title 'The Plant Lytic Vacuole: Space-Filler, Garbage Bag, or Something More Interesting?'
Year(s) Of Engagement Activity 2017
 
Description Invited speaker at York University 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact Invited speaker to York University: Plant Biology Seminar - The Plant Lytic Vacuole: Space-Filler, Garbage Bag, or Something More Interesting?
Year(s) Of Engagement Activity 2017
 
Description JIC50 public open day 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Open Day at JIC celebrating 50 years on the Norwich site. Soil columns featuring the N sensors were displayed to the public.
Year(s) Of Engagement Activity 2017
URL https://www.jic.ac.uk/news-and-events/blog-copy/2017/08/john-innes-celebrates-50-years-norwich-2017
 
Description Keynote Speaker Future Food Festival Toowoomba Queensland Australia 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact I was a Keynote Speaker at the Future Food Festival Toowoomba Queensland Australia, giving 3 presentations to scientists, two to school kids and one grand discovery presentation at Queensland University of Technology to the general public
Year(s) Of Engagement Activity 2018
 
Description Keynote Speech: Plants and Human Health at the Wellcome Trust China-UK Life Science Summit 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Keynote Speech: Plants and Human Health at the Wellcome Trust China-UK Life Science Summit
Year(s) Of Engagement Activity 2017
 
Description LEGATO-EUROLEGUME International Conference - Advances in grain legume cultivation and use 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact Attendance at LEGATO-EUROLEGUME International Conference - Advances in grain legume cultivation and use
Year(s) Of Engagement Activity 2017
 
Description London International youth science Forum 2019 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Undergraduate students
Results and Impact About 30 students visited the John Innes Centre. I presented a short talk about starch research, followed by a discussion.
Year(s) Of Engagement Activity 2019
 
Description MRC Confidence in Global Nutrition Skype conference: The Role of Nutrients, Gut Dysfunction and the Gut Microbiome in Determining Health Outcomes in Undernutrition. 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact Project collaborator Skype conference meeting on The Role of Nutrients, Gut Dysfunction and the Gut Microbiome in Determining Health Outcomes in Undernutrition.
Year(s) Of Engagement Activity 2018
 
Description Magnesium Homeostasis in Plants: Towards finding sensing and -signalling Factors 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Presentation at Gatsby Network Meeting - Oxford. Annual event. Presentaion - Magnesium Homeostasis in Plants: Towards finding sensing and -signalling Factors
Year(s) Of Engagement Activity 2019
 
Description Mardlers visit to JIC. Overview of projects talk given. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Mardlers - the Norfolk farmers group of which JIC is an official member, meet once a month to listen to outside speakers and discuss various issues.
Year(s) Of Engagement Activity 2018
 
Description Meeting with Adrian Russell, International pea breeder, and James Wallace, IAR Agri 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Meeting with Adrian Russell, International pea breeder, and James Wallace, IAR Agri
Year(s) Of Engagement Activity 2018
 
Description Meeting with Alfonso Clemente, Spanish National Research Council (CSIC), Experimental Station of Zaidín to discuss possible collaboration 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact Meeting with Alfonso Clemente, Spanish National Research Council (CSIC), Experimental Station of Zaidín to discuss possible collaboration
Year(s) Of Engagement Activity 2018
 
Description Meeting with Elsoms/Crites 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Meeting with Elsoms/Crites
Year(s) Of Engagement Activity 2018
 
Description Meeting with Keith Costello and James Wallace, Pea Yield Enhancement Network (PeaYEN) 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Meeting with Keith Costello and James Wallace, Pea Yield Enhancement Network (PeaYEN)
Year(s) Of Engagement Activity 2018
 
Description Meeting with MRC project collaborators 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Other audiences
Results and Impact Meeting with MRC project collaborators
Year(s) Of Engagement Activity 2018
 
Description Meeting with Novozymes Industry representatives 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact Discuss future collaborations with Novozymes representatives (led by Jeanne Kjaer). Skype call with contacts in USA and India. (11 March 2019)
I presented my current research and we discussed opportunites for future collaboration.
Meeting facilitated by Jon Clarke at JIC.
Year(s) Of Engagement Activity 2019
 
Description Meeting with Seb from BRAVE 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Telephone meeting with Seb from BRAVE regarding getting people to eat more British-grown pulses and using roasted peas as a healthy and sustainable snack.
Year(s) Of Engagement Activity 2018
 
Description Meeting with Will Pillinger, Limagrain UK 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Meeting with Will Pillinger, Limagrain UK
Year(s) Of Engagement Activity 2018
 
Description Meeting with project collaborators regarding BBSRC Food call 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Other audiences
Results and Impact Meeting with project collaborators regarding BBSRC Food call
Year(s) Of Engagement Activity 2019
 
Description Member of the UK Science Partnership for Animal and Plant Health 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Member of the UK Science Partnership for Animal and Plant Health - quarterly meetings are held
Year(s) Of Engagement Activity 2017
 
Description Member of the interviewing panel for Herchel Smith Professorship of Biochemistry 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact Member of the interviewing panel for Herchel Smith Professorship of Biochemistry position - University of Cambridge
Year(s) Of Engagement Activity 2017
 
Description Membership of Scientific Advisory Committee to the Plant Energy Biology ARC Centre of Excellence 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Membership of Scientific Advisory Committee to the Plant Energy Biology ARC Centre of Excellence. An Australian National Centre.
Year(s) Of Engagement Activity 2017
 
Description New Phytologist Next Generation Scientists meeting - Open Address 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Opening Address at New Phytologist Next Generation Scientists meeting - Scientific meeting
Year(s) Of Engagement Activity 2017
 
Description News from the Pulse Crop Genetic Improvement Network (PCGIN). PGRO Pulse magazine 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Other audiences
Results and Impact News from the Pulse Crop Genetic Improvement Network (PCGIN). PGRO Pulse magazine, spring 2018, p. 14
Year(s) Of Engagement Activity 2018
 
Description Norfolk Mardlers Dinner 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Mardlers - the Norfolk farmers group of which JIC is an official member, meet once a month to listen to outside speakers and discuss various issues.
Year(s) Of Engagement Activity 2018
 
Description Norwich Science Festival 2023 - Stall about starch research 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact We ran an interactive stall that communicated the importance of starch research in wheat and potatoes, and the potential impacts of the research on crop productivity and quality. Members of the team were available to answer questions from the attendees.
Year(s) Of Engagement Activity 2023
 
Description Norwich Science Festival Training week 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Schools
Results and Impact Parties of school children from across the region visited the Norwich Science Festival held in the Forum in Norwich city centre on the 18/19 October 2017. We displayed zinc staining of various different types of food grains to show the zinc rich parts of the seed. The display was also visited by members of the public who passed through the Forum.
Year(s) Of Engagement Activity 2017
 
Description Norwich and Norfolk Science Festival - Starch Stall 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Members of my laboratory, led by a postdoctoral scientist in my group, set up a stall to discuss the importance of starch research with the general public at the Norwich Science Festival. This involved several demonstrations and posters, aimed at both young children as well as adults. Questions from the general public about starch were answered.
Year(s) Of Engagement Activity 2019
 
Description Nourishing ten billion sustainably: resilient food production in a time of climate change 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact Talk to retired medical doctors.
Year(s) Of Engagement Activity 2022
 
Description Nourishing ten billion sustainably: resilient food production in a time of climate change 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Policymakers/politicians
Results and Impact CLIMATE CHANGE : SCIENCE AND SOLUTIONS | BRIEFING 10
The global food system accounts for around one third of greenhouse gas (GHG) emissions generated by human activity. It therefore offers a major opportunity for progress towards net zero if emissions can be reduced at the same time as delivering food security and building resilience to the inevitable impacts of climate change. Research shows
how solutions can be found in diet change, respectfully approached, sustainable agricultural practices and harnessing the continuing wave of innovation in food biotechnology
Year(s) Of Engagement Activity 2021
URL https://royalsociety.org/-/media/policy/projects/climate-change-science-solutions/climate-science-so...
 
Description Open Day at Bawburgh field station 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact Open Day to engage the local community with the JIC field station, including information on planned GM crop field trials. There was an excellent turnout of all ages.
Year(s) Of Engagement Activity 2021
 
Description Opening address at Breeders Day 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Opening address at Breeders Day at the John Innes Centre
Year(s) Of Engagement Activity 2017
 
Description PAG2022 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Media (as a channel to the public)
Results and Impact Talk at the Plant and Animal Genome Conference 2022
Year(s) Of Engagement Activity 2022
 
Description Panel Meeting of Severo Ochoa and Maria de Maeztu Committee - Madrid, Spain 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Professional Practitioners
Results and Impact Panel Meeting of Severo Ochoa and Maria de Maeztu Committee - Madrid, Spain
Year(s) Of Engagement Activity 2017
 
Description Panel discussion for the Royal Society for Hay Festival 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Hay Festival - panel discussion for the Royal Society
exploring the public attitudes to the range of applications that genetic technologies might have, and the differences in such attitudes depending on context. This might look at the difference in public opinion in areas like food and medicine, for example, or explore concepts like 'naturalness'
Year(s) Of Engagement Activity 2017
 
Description Pea Yield Enhancement Network (PeaYEN) 2018 review meeting 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Meeting with Pea Yield Enhancement Network (PeaYEN) representative
Year(s) Of Engagement Activity 2018
 
Description Pea Yield Enhancement Network (PeaYEN) 2018 review meetings 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Other audiences
Results and Impact Pea Yield Enhancement Network (PeaYEN) 2018 review meetings
Year(s) Of Engagement Activity 2018
 
Description Pea Yield Enhancement Network (PeaYEN) annual review meeting 2018 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Other audiences
Results and Impact Pea Yield Enhancement Network (PeaYEN) annual review meeting 2018
Year(s) Of Engagement Activity 2018
 
Description Pea display at Gardeners' Question Time 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Public recording event of BBC Radio 4 Gardeners' Question Time held at JIC. Pea display for visitor to view.
Year(s) Of Engagement Activity 2018
 
Description Pea is for potential. In: JIC Advances volume 30, Winter 2018/19. Peas; past and present, pp. 14-15 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Other audiences
Results and Impact Pea is for potential. In: JIC Advances volume 30, Winter 2018/19. Peas; past and present, pp. 14-15
Year(s) Of Engagement Activity 2018
 
Description PeaGen meeting with project collaborators 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact PeaGen meeting with project collaborators
Year(s) Of Engagement Activity 2019
 
Description PeaGen project meeting 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact PeaGen project meeting
Year(s) Of Engagement Activity 2019
 
Description Plenary Speaker IAPB Congress Dublin Ireland 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact I was a Plenary Speaker at the IAPB Congress Dublin Ireland
Year(s) Of Engagement Activity 2018
 
Description Plenary Speaker SEB Meeting Florence 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Plenary speaker at SEB Meeting in Florence. England beat Colombia on penalties in the World Cup.
Year(s) Of Engagement Activity 2018
 
Description Plenary Speaker at Foods of the Future Workshop in Koln 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact I was a Plenary Speaker at Foods of the Future Workshop in Koln
Year(s) Of Engagement Activity 2018
 
Description Plenary speaker, International meeting on plant genomics, Verona Italy 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact I was a Plenary speaker, at the International meeting on plant genomics, Verona Italy
Year(s) Of Engagement Activity 2018
 
Description Poster presentation at Annual Zinc Net Meeting (Cambridge UK) 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Poster title of presentation: Finding new components of the zinc homeostasis network in Arabidopsis.
Authors: Stanton C., Sanders D., Miller T.
Year(s) Of Engagement Activity 2019
 
Description Powering Pea Productivity - Realising the Potential for UK agriculture workshop 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Invitation-only workshop (organised by Agri-Tech East, hosted by the John Innes Centre and sponsored by BBSRC, with the support of PGRO) This event will include representatives from various communities across the value chain for peas, including growers, agronomists, agrochemical companies, processors, researchers, seed companies, breeders and natural product companies. Companies signed up to attend include Syngenta, Hutchinsons, Anglia Pea Growers, and Yara.
Year(s) Of Engagement Activity 2019
 
Description Presentation at Chinese University Lecturers Conference - Fujian Agricultural University 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Miller A. J.(2018) Nitrogen uptake and assimialtion in Crop Plants (2 lectures) Fujian Agriculture and Forestry University, Fuzhou, China. Chinese University Lecturers Conference
Year(s) Of Engagement Activity 2019
 
Description Presentation at Mardlers dinner 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Industry/Business
Results and Impact The Mardlers are a group of approximately 25-30 Norfolk farmers, which all have reach beyond the farm gate, either through processing and marketing of their crops or stock, or through membership of industry bodies, political, research or marketing. There are also members who are involved with banking, accountancy and land agency, and as such they have a very broad range of interests across the agricultural, food and business sectors, both regionally and nationally.
Year(s) Of Engagement Activity 2018
 
Description Presentation at UK-China Workshop 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact 15-17 October Workshop at UEA. Chinese representatives from Anhui Agricultural University (AAU) and Jiangsu Academy of Agricultural Sciences (JAAS). Meeting organized by Dr Yuelai Lu (SAIN, School of International Development, UEA). Title of presentation: Soil Nitrogen and Crops
Year(s) Of Engagement Activity 2018
 
Description Presentation at the European Parliament Plants, Diet and Health 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Policymakers/politicians
Results and Impact Presentation to the European parliament on the importance of fruit and vegetables in the diet. we discussed how best to implement lower costs, greater access and greater consumption of fruit and vegetables.
Year(s) Of Engagement Activity 2018
 
Description Presentation at the annual NABIM meeting 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact I presented the research on our "high-iron wheat" to British and Irish Millers. This sparked discussion on whether we should allow GM crops in Britain.
Year(s) Of Engagement Activity 2018
 
Description Presentation on Pulse Crop Genetic Improvement Network (PCGIN) given to Clare Moriarty and Murry Santra from Defra 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Policymakers/politicians
Results and Impact Presentation on Pulse Crop Genetic Improvement Network (PCGIN) given to Clare Moriarty and Murry Santra from Defra
Year(s) Of Engagement Activity 2018
 
Description Presentation to JIC Governing Council: 'Translating Genetic Research In Species Pisum: from MfN to Breeders and Beyond' 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Supporters
Results and Impact Presentation to JIC Governing Council: 'Translating Genetic Research In Species Pisum: from MfN to Breeders and Beyond'
Year(s) Of Engagement Activity 2017
 
Description Presentation to Suffolk Organic Gardners, Bury St Edmunds 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact 'Soils: root/microbial relationships and nutrient cycling' - presentation to the Suffolk Organic Gardeners Association in Bury St Edmunds (11 th February 2020).
Year(s) Of Engagement Activity 2020
URL https://www.gardenorganic.org.uk/suffolk-organic-gardeners
 
Description Press article - The Pulse Magazine - The Official Journal of the PGRO 
Form Of Engagement Activity A magazine, newsletter or online publication
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Other audiences
Results and Impact The Pulse Magazine - The Official Journal of the PGRO. The John Innes Centre celebrates 50 Years in Norwich in 2017
Year(s) Of Engagement Activity 2017
 
Description PrimeAg visit 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Professional Practitioners
Results and Impact Eight visitors from PrimeAg visited JIC for half a day. Prime Agriculture LLP is a consultancy partnership of 11 BASIS and FACTS qualified agronomists, working with growers in in Essex, Suffolk, Norfolk, Cambridgeshire, Bedfordshire, Northamptonshire and Hertfordshire. http://www.primeag.co.uk/
Year(s) Of Engagement Activity 2018
URL http://www.primeag.co.uk/
 
Description Pulse Crop Genetic Improvement Network (PCGIN) Stakeholder Meeting 2017 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Pulse Crop Genetic Improvement Network (PCGIN) Stakeholder Meeting
Year(s) Of Engagement Activity 2017
 
Description Pulse Crop Genetic Improvement Network (PCGIN) Stakeholder Meeting 2018 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Meeting with Pulse Crop Genetic Improvement Network (PCGIN) stakeholders, including representatives from UK government, industry, universities and research institutes.
Year(s) Of Engagement Activity 2018
 
Description Pulse Crop Genetic Improvement Network (PCGIN) Stakeholder Meeting 2019 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Meeting with Pulse Crop Genetic Improvement Network (PCGIN) stakeholders, including representatives from UK government, industry, universities and research institutes.
Year(s) Of Engagement Activity 2019
 
Description Pulse Crop Genetic Improvement Network (PCGIN) steering/management meeting 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Pulse Crop Genetic Improvement Network (PCGIN) steering/management meeting
Year(s) Of Engagement Activity 2018
 
Description Pulse Crop Genetic Improvement Network (PCGIN) steering/management meeting 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Pulse Crop Genetic Improvement Network (PCGIN) steering/management meeting
Year(s) Of Engagement Activity 2019
 
Description Pulse Crop Genetic Improvement Network (PCGIN) steering/management meeting 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Pulse Crop Genetic Improvement Network (PCGIN) steering/management meeting
Year(s) Of Engagement Activity 2019
 
Description Pulse crop breeders' workshop meeting at Limagrain UK 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact Attendance at the Pulse crop breeders' workshop meeting at Limagrain UK to discuss possible collaborations.
Year(s) Of Engagement Activity 2018
 
Description Quadram Institute Bioscience Trustees/ BBSRC conference 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Joint presentation 'Resistant starch and the food chain' (with Dr Fred Warren, QIB) to QIB trustees, BBSRC and industry representatives
Year(s) Of Engagement Activity 2022
 
Description Radio broadcast - BBC Radio Norfolk 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Radio broadcast on BBC Radio Norfolk. JIC Open Day 2017: 'The application of genetic research in pea at JIC to the development of novel foods' - hosted by Thordis Fridriksson
Year(s) Of Engagement Activity 2017
 
Description Radio interview for BBC Radio 4 Gardeners' Question Time programme 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Radio interview for BBC Radio 4 Gardeners' Question Time programme
Year(s) Of Engagement Activity 2018
 
Description Royal Norfolk Show 2022 - Display about starch research 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact We ran an interactive stall that demonstrated the importance of starch research, especially in wheat and potatoes. We communicated the implications of starch research on crop quality and human health. The activities were aimed primarily at school children, but many parents, teachers and other members of the general public attended and asked questions about our research, and about starch in general.
Year(s) Of Engagement Activity 2022
 
Description Royal Society Meeting for New Fellows introductory presentation 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact I presented my research that underpinned my election as a Fellow of the Royal Socity
Year(s) Of Engagement Activity 2018
 
Description Royal Society fact finding visit to Beijing 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Royal Society fact finding visit to Beijing, 22 to 26 October for Royal Society's genetic technologies programme
Year(s) Of Engagement Activity 2017
 
Description School Visit at Sir Isaac Newton sixth form science day 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact A postdoctoral scientist in my laboratory organised and led a stall about starch at Sir Isaac Newton sixth form, for their Science day. The stall had hand-on activities, displays and posters for demonstrating the importance of starch, and the goals of starch research.
Year(s) Of Engagement Activity 2019
 
Description Scientific Advisory Board Centre for Agricultural Genomics (CRAG) Barcelona 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I chaired the Scientific Advisory Board Centre for Agricultural Genomics (CRAG) Barcelona, and compiled the report for the institution. I also set up the twinning program between JIC and CRAG through liaison with the director, Jose-Luis Reichmann
Year(s) Of Engagement Activity 2018
 
Description Scientific Advisory Board Meeting, on Banana Biofortification Gates project 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I advised on scientif direction for the Biofortification of Banana project funded by BMGF in Kampala Uganda
Year(s) Of Engagement Activity 2018
 
Description Scientific Advisory Board Member for Max PIanck Institute of Molecular Plant Physiology, Golm, Germany 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Scientific Advisory Board Meeting for Max PIanck Institute of Molecular Plant Physiology
Year(s) Of Engagement Activity 2017
 
Description Scientific Advisory Board Plant and Food Research, New Zealand 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Policymakers/politicians
Results and Impact I attended the Scientific Advisory Board Meeting Plant and Food Research, New Zealand, listened to and advised their emerging scientists and advised on scientific policy.
Year(s) Of Engagement Activity 2018
 
Description Second Defra Research Advisory Group meeting 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Policymakers/politicians
Results and Impact Meeting with Defra and AHDB representatives, along with Research Advisory Group members and Genetic Improvement Network leads.
Year(s) Of Engagement Activity 2018
 
Description Soil and Plant conference 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Presentation was titled: Soil health and nutrient supply to plants.
Soil-Plant-Atmosphere Continuum 2015 Conference
Year(s) Of Engagement Activity 2015
 
Description Study tour visit from fellows of the Royal Agricultural Society England (RASE) 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Study tour visit from fellows of the Royal Agricultural Society England (RASE)
Year(s) Of Engagement Activity 2019
 
Description TV Broadcast - BBC Look East 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact TV broadcast on BBC Look East - JIC Open Day 2017: 'The application of genetic research in pea at JIC to its many uses in food and agriculture'
Year(s) Of Engagement Activity 2017
 
Description Tele-con meeting with Helen Holmes, ADAS and representatives from Askew & Barrett's to discuss a possible project 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Policymakers/politicians
Results and Impact Tele-con meeting with Helen Holmes, ADAS and representatives from Askew & Barrett's to discuss a possible project
Year(s) Of Engagement Activity 2018
 
Description Telephone meeting with Pankaj Bhowmik, National Research Council of Canada 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact Telephone meeting with Pankaj Bhowmik, National Research Council of Canada. Meeting led to a joint Canada-UK proposal being submitted in early 2019.
Year(s) Of Engagement Activity 2018
 
Description Telephone meeting with Pankaj Bhowmik, National Research Council of Canada. 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact Telephone meeting with Pankaj Bhowmik, National Research Council of Canada. Meeting led to a joint Canada-UK proposal being submitted in early 2019.
Year(s) Of Engagement Activity 2018
 
Description Tour of Pea Cage and Dorothea de Winton building at Church Farm - for JIC and QIB staff. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Other audiences
Results and Impact Tour of Pea Cage and Dorothea de Winton building at Church Farm - for JIC and QIB staff.
Year(s) Of Engagement Activity 2019
 
Description Transatlantic Sessions on Conservation and Organic Agriculture 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact PPP: Potential and Power of Pea presentation at Transatlantic Sessions on Conservation and Organic Agriculture
Year(s) Of Engagement Activity 2018
 
Description UK Legume Research Community Steering Group meeting at MRC in London 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Other audiences
Results and Impact UK Legume Research Community Steering Group meeting at MRC in London
Year(s) Of Engagement Activity 2019
 
Description UK Legume community genetics and genomics resources workshop 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact UK Legume community genetics and genomics resources workshop
Year(s) Of Engagement Activity 2018
 
Description University of Geneva - PhD Retreat 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Postgraduate students
Results and Impact University of Geneva - PhD Retreat, participation in a debate: " Technological advances - A new chance for changing public perception on GMOs". Also speaker title: 'The plant lytic vacuole: space-filler, garbage bag, or something more interesting'. A two-day retreat for PhD students in molecular plant sciences at universities in French-speaking Switzerland.
Year(s) Of Engagement Activity 2017
 
Description Using crop genetics to understand the importance of dietary resistant starches for maintaining healthy glucose homeostasis (CRESTAR) Industrial Stakeholder Meeting 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Using crop genetics to understand the importance of dietary resistant starches for maintaining healthy glucose homeostasis (CRESTAR) Industrial Stakeholder Meeting
Year(s) Of Engagement Activity 2017
 
Description Visit of 30 undergraduate students from Wageningen University, The Netherlands 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Undergraduate students
Results and Impact Tour of JIC caged pea plot area - visit of 30 undergraduate students from Wageningen University, The Netherlands
Year(s) Of Engagement Activity 2017
 
Description Visit of Professor Ian Boyd, Chief Scientific Adviser, Defra to JIC 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Policymakers/politicians
Results and Impact Visit of Professor Ian Boyd, Chief Scientific Adviser, Defra to JIC
Year(s) Of Engagement Activity 2019
 
Description Visit of delegates from Oost NL organised by New Anglia 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact 15 delegates from food-related companies that are part of Oost NL (East Netherlands Development Agency) came to visit the John Innes Centre to hear about the latest research that is relevant to the food industry. They were joined by delegates from the embassy of the Netherlands in the United Kingdom and DEFRA. My talk raised awareness of the main challenges in basic starch research, and its potential to improve food quality.
Year(s) Of Engagement Activity 2021
 
Description Visit to Domoney lab by a PhD candidate with University of Cape Town in South Africa 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Visit to Domoney lab by a PhD candidate with University of Cape Town in South Africa. The PhD student is working on on Thermotolerance genotypes for chickpea production in NE South Africa. Discussion on pea.
Year(s) Of Engagement Activity 2019
 
Description Visit to JIC by Marks and Spencer 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Meeting with members of Marks and Spencer
Year(s) Of Engagement Activity 2019
 
Description Visit to Metabolic Biology department by Professor Gregory Radick, Professor of History and Philosophy of Science at the University of Leeds. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Other audiences
Results and Impact Visit to Metabolic Biology department by Professor Gregory Radick, Professor of History and Philosophy of Science at the University of Leeds.
Year(s) Of Engagement Activity 2019
 
Description Workshop on UN Sustainable Development Goals 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Workshop organised by EPSO on UN SDGs and how Plant Science could contribute
Year(s) Of Engagement Activity 2018
 
Description World Life Science Conference (Sustainable Agriculture Session), Beijing, 2018 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact China National Convention Center presentation: The Plant Vacuole: Roles in Plant and Human Nutrition, and Cellular Signaling 27th - 29th October 2018
Year(s) Of Engagement Activity 2018