BBSRC National Bioscience Research Infrastructure: Low Containment Services and Science Platforms

Lead Research Organisation: The Pirbright Institute
Department Name: UNLISTED

Abstract

Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.

Technical Summary

The purpose of the Low Containment Services and Science Technology Platforms capability is to both support the High Containment Services and Science Technology Platforms (PIR-NBRI-1) and to provide dedicated facilities for low containment study of virus: host interactions. The NBRI includes five major pieces of infrastructure: The BBSRC National Vaccinology Centre: The Jenner Building two insectary facilities (The Philip Mellor Insectary and the IS4L facility) The Houghton Facility a poultry hatchery unit and The Biggs Building a low containment facility for infection of poultry. The Jenner building houses around 100 scientists in low containment laboratories and offices and includes those studying poultry viruses that are endemic in the UK (including low pathogenicity animal influenza viruses Marek’s disease virus and infectious bronchitis viruses) and scientists performing fundamental host-pathogen interaction studies including those developing genetically modified insect lines. Jenner also supports scientists who primarily work in high containment (under our PIR-NBRI-1 application) who require low containment space for some tasks and bioinformaticians. The other four infrastructures provide dedicated specialist services for production maintenance and infection of poultry and insects. The NBRI supports large and diverse teams of workers in engineering risk and assurance and animal services that rigorously maintain the highest standards in running of low containment facilities requiring highly specialised controlled temperature regimes and animal husbandry.

A range of science platforms are also maintained in Jenner as part of the NBRI. The Immunological Toolbox facility provides a diverse range of unique reagents and resources to promote veterinary immunology and vaccinology research both in the UK and globally. In addition bioimaging flow cytometry and bioinformatics services are provided that allow comparable data for studies carried out across containment levels and harmonisation in analysis and interpretation. Together these science platforms not only provide vital collaborative support during studies but also provide training to a wide range of users helping to sustain the UK’s excellence in bioscience.

Planned Impact

unavailable
 
Description Imperial College London (Masters-level training)
Geographic Reach Multiple continents/international 
Policy Influence Type Influenced training of practitioners or researchers
Impact The students will be better prepared for how to prepare and respond to outbreaks of tropical vector-borne diseases, which is highly relevant as many students on the course were from disease-endemic countries.
URL https://www.imperial.ac.uk
 
Description University of Oxford (Medical students training)
Geographic Reach National 
Policy Influence Type Influenced training of practitioners or researchers
Impact Medical students in the UK may encounter tropical diseases in patients returning from overseas travel. In this lecture they gained an insight into the disease caused by dengue virus and how people can protect themselves when they travel overseas, allowing them to better care for patients when they enter the clinic.
URL https://www.ox.ac.uk
 
Description Zika pandemic preparedness ten-year plan consultation (Wellcome Trust/WHO)
Geographic Reach Multiple continents/international 
Policy Influence Type Participation in a guidance/advisory committee
 
Description Application of molecular xenomonitoring in a lateral flow test format for multiplexed monitoring of vector-borne viruses and their animal reservoirs and insect vectors in low-income settings
Amount £49,015 (GBP)
Funding ID IAA2149 
Organisation Medical Research Council (MRC) 
Sector Public
Country United Kingdom
Start 03/2024 
End 03/2025
 
Description Blood Markers for prediction of respiratory virus infection
Amount £21,000 (GBP)
Organisation The Pirbright Institute 
Sector Academic/University
Country United Kingdom
Start 05/2023 
End 07/2024
 
Description Development of CVI988 based recombinant vaccine
Amount £238,000 (GBP)
Organisation Eco Animal Health Ltd 
Sector Private
Country United Kingdom
Start 03/2023 
End 02/2024
 
Description Development of avian herpesvirus vector vaccines for poultry
Amount £772,000 (GBP)
Organisation MSD Animal Health 
Sector Private
Country United Kingdom
Start 01/2024 
End 12/2026
 
Description Effects of co-infections on Marek's disease in poultry and development of novel recombinant Marek's disease virus vector vaccines
Amount £562,000 (GBP)
Funding ID BB/X017575/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 09/2023 
End 09/2026
 
Description Emerging porcine influenza and coronaviruses" (EPICVIR)
Amount £596,027 (GBP)
Funding ID BB/X019780/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 11/2023 
End 10/2026
 
Description MRC Impact Accelerator Accounts (Tropical Infectious Disease Consortium: Expanding and Accelerating Product Development)
Amount £53,362 (GBP)
Funding ID IAA2148 
Organisation Medical Research Council (MRC) 
Sector Public
Country United Kingdom
Start 03/2024 
End 02/2025
 
Description Travel Grant
Amount £350 (GBP)
Organisation Microbiology Society 
Sector Learned Society
Country United Kingdom
Start 03/2024 
End 04/2024
 
Description Using machine learning to identify the molecular determinants of vector-borne transmission in viruses
Amount £110,327 (GBP)
Organisation The Pirbright Institute 
Sector Academic/University
Country United Kingdom
Start 09/2024 
End 04/2028
 
Title Development of recomibinant system to express pig IgM and IgA 
Description This system allows the recombinant expression oligomeric antibodies (IgM & IgA) from pig. 
Type Of Material Technology assay or reagent 
Year Produced 2023 
Provided To Others? No  
Impact No impact yet 
 
Title Development of recomibinant system to express rabbit IgG 
Description This system has been developed to express recombinant IgG from rabbit B cells 
Type Of Material Technology assay or reagent 
Year Produced 2024 
Provided To Others? Yes  
Impact No impact yet 
 
Title Establishment of an efficient CRISPR/Cas9 gRNA library screening to identify the host factors required for maintaining the transformed phenotype of MDV tumour cell lines 
Description Herpesviruses are large dsDNA viruses that cause widespread, lifelong latent infections in different hosts, through multiple virus-host interactions to create a delicate balance between the virus and the host. CRISPR/Cas9-based gene editing is emerging as a powerful tool to investigate the precise determinants of latency in a number of herpesvirus infections. Marek's disease virus (MDV-1) is a lymphotropic a-herpesvirus associated with latent infections and malignant CD4+ T-cell lymphomas in chicken. MDV-1 has a two-phase life cycle, consisting of a lytic and a latent phase, the latter closely associated with the oncogenesis of the virus, yet the underlying molecular mechanisms of cell transformation remain unclear. Better understanding of the factors that maintain the latency of the virus will provide insights into novel intervention strategies to interfere with the neoplastic process. We have used a high throughput genome-wide CRISPR/Cas9 gene knockout strategy, combined with next generation sequencing to identify the genes critical for maintenance of the transformed phenotype. 339 host genes have been identified being essential for maintaining the transformed phenotype of MDV cell line. 
Type Of Material Technology assay or reagent 
Year Produced 2023 
Provided To Others? No  
Impact Establishment of the pipeline of CRISPR library screening in chicken cell lines has given the opportunity for the identification factors associated with disease pathogenesis and neoplastic transformation. The identified genes could serve as targets for inducing de novo genetic resistance using gene editing approaches. 
 
Title Establishment of an efficient CRISPR/Cas9-based screening pipeline for herpesviruses to study the gene function and streamline the vector platform for recombinant vaccine development 
Description The development of both cosmid DNA and Bacterial artificial chromosome (BAC) technologies has greatly facilitated the introduction of mutations into the viral genomes of herpesviruses to study gene functions. However, cloning of viral genomes as BAC plasmid and subsequent mutagenesis is inefficient, time-consuming and may introduce mutations by repeated passages. Following our success in efficient CRISPR/Cas9 editing of the MDV genome in both replicating virus and MDV transformed cell lines and of HVT genome for the recombinant vaccine development, we have developed an efficient CRISPR/Cas9-based screening pipeline for herpesviruses to identify the essential/non-essential genes for study of the gene function and streamline the vector platform for recombinant vaccine development. 
Type Of Material Technology assay or reagent 
Year Produced 2022 
Provided To Others? No  
Impact Identification of essential and non-essential genes of herpesviruses provided opportunities to study gene function in vitro and in vivo. Knocking out the non-essential genes in the HVT vector can enhance HVT replication in vivo enabling recombinant HVT-based vaccines to induce stronger immune responses. 
 
Title Lateral flow detection of avian leukosis viruses and Reticuloendotheliosis virus 
Description The system for detecting ALV/REV based on CRISPR-Cas13a, a SHERLOCK reaction system consists of specific RPA primers for amplifying target nucleic acid fragments, Cas13a protein, crRNA, and Lateral flow chromatography test strip for visualized display of results. The assay started with pre-amplification of either a DNA or RNA target input. Amplified targets are then converted to RNA via T7 transcription and detected by Cas13-crRNA complexes, which activate and cleave fluorescent RNA reporters based on its collateral cleavage nature. The result can be visualized using colorimetric lateral flow reaction. The test is rapid, sensitive and specific for detection of ALV/REV at 37°C. The product can commercialized with a great clinical application prospects. 
Type Of Material Technology assay or reagent 
Year Produced 2020 
Provided To Others? No  
Impact The early identi?cation and removal of virus-shedding birds are essential to reduce the transmission. This rapid, simple-to-use and cost effective on-site diagnostic method will aid the eradication of ALV/REV in the affected area. 
 
Title The establishment of an efficient pipeline for in situ CRISPR editing of the Marek's disease virus (MDV) genome in lymphoma-derived cell lines 
Description The lymphoblastoid cell lines (LCLs) derived from MD lymphomas have served as valuable resources to study virus-host molecular interactions in transformed cells. However, detailed investigations into the functional role of different viral and host determinants in these cells have been difficult due to the lack of tools for in situ manipulation of viral/host genomes in MDV-transformed cell lines. Our recent success in efficient CRISPR/Cas9 editing of the MDV genome in LCLs has demonstrated the potential for targeted editing to dissect the regulatory pathways involved in latency, transformation, reactivation and lytic switch. 
Type Of Material Technology assay or reagent 
Year Produced 2018 
Provided To Others? Yes  
Impact Using this approach, we have demonstrated viral genes such as pp38 and MDV-miR-M4 are not essential for maintaining the transformed phenotype. On the other hand, deletion of MDV-encoded oncogene Meq or mutations preventing its interactions resulted in cell death demonstrating the essential role of Meq in transformation. 
URL https://www.mdpi.com/1999-4915/10/6/279
 
Title scRNA seq analysis of porcine BAL 
Description We describe for the first time scRNA-seq analysis of porcine bronchoalveolar lavage (BAL), a cell source increasingly used to analyse respiratory immune responses, and which has been shown to be major correlate for protection against respiratory infections such as influenza, respiratory syncytial virus, and SARS-CoV-2. Our work reveals both similar and unique cell subsets and divergent transcriptome profiles of BAL immune cells compared to publicly available data from blood cells. 
Type Of Material Technology assay or reagent 
Year Produced 2024 
Provided To Others? Yes  
Impact The data we have generated will provide an atlas for future pig BAL scRNA-seq studies. 
 
Title Spatial transcriptomics of Chicken trachea infected with Infectious bronchitis virus to identify transcriptomic signatures 
Description Rhode Island Red SPF chickens were infected with M41-CK (a cell culture adapted pathogenic IBV virus) to investogate the evolution of the virus in chickens after infection, and compare with live attenuated vaccine versions of the same virus. Illumina sequence datasets were piped into the 10X proprietary analysis pipeline 'Space Ranger' for reads alignments, feature barcode matrices generation and secondary analysis including clustering and differential gene expression. Outputs from Space Ranger consisting of tissue images, tissue positions and feature barcode matrix were further piped into previously published R-based spatial pipeline 'Giotto' (Dries et al, 2021) for detailed analysis such as dimensionality reduction, co-visualization of gene expression and spatial location, cell type marker gene detection, spatial structure analysis, spatial gene co-expression patterns, etc. 
Type Of Material Database/Collection of data 
Year Produced 2024 
Provided To Others? No  
Impact The dataset will have impact for other research groups investigating avian coronavirus vaccines. They will also have a broader impact and be of interest to wider coronavirus vaccine development and research thorough the information on vaccine evolution and host response. The datasets will be of interest to persons within the field of spatial biology field, notably the software developers and bioinformaticians, as a dataset to test new models and algorithms. The results will be of interest specifically for those in industry who are involved in developing novel and more rational strategies for developing vaccines. 
 
Description Andrew Davidson - Proteome of viral replication organelles 
Organisation University of Bristol
Country United Kingdom 
Sector Academic/University 
PI Contribution Projects are being initiated to study the cellular proteins associated with coronavirus replication organelle membranes and a funding application has been submitted to BBSRC.
Collaborator Contribution Andrew has provided assistance with experimental planning and drafting of a grant application. He has also provided protocols to allow preliminary data to be generated. Once protocols have been established, Andrew will facilitate mass spectrometry data generation and will analyse the resulting data.
Impact No outcomes to date, projects being initiated and funding applications in progress.
Start Year 2018
 
Description Cambridge 
Organisation University of Cambridge
Country United Kingdom 
Sector Academic/University 
PI Contribution Collaborative research on epitope display systems and use of functional genetic screens.
Collaborator Contribution Collaborative research on epitope display systems and use of functional genetic screens.
Impact Collaborative research on epitope display systems and use of functional genetic screens.
Start Year 2022
 
Description Collaboration with USDA to seuence anti-pig immune protein hybridomas 
Organisation U.S. Department of Agriculture USDA
Country United States 
Sector Public 
PI Contribution Sequenced 14 mouse anti-pig immune protein hybridomas.
Collaborator Contribution Shared hybridomas for sequencing and agreed to post availbility of these recombinant antibodies on immunological toolbox website
Impact Preservation of important immune reagents as sequences, which will also faciliate sharing of these reagents.
Start Year 2023
 
Description Collaboration with WSU to seuence anti-bovine immune protein hybridomas 
Organisation Washington State University
Country United States 
Sector Academic/University 
PI Contribution Sequenced 15 mouse anti-bovine immune protein hybridomas.
Collaborator Contribution Shared hybridomas for sequenceing and agree to post availiity of these reagents as recombinant on the immunological toolbox website for wider community
Impact Permanently secured these hynrdiomas as antibody sequences
Start Year 2023
 
Description Dan Clare - cryo-electron microscopy 
Organisation Diamond Light Source
Country United Kingdom 
Sector Private 
PI Contribution The project aims to characterise proteins complexes associated with coronavirus replication organelles. My team provides significant expertise in replication organelles induced by coronaviruses. Laboratory work is performed by my team. The project is funded by a joint PhD studentship working, predominantly in my laboratory.
Collaborator Contribution Dan provides expertise in cryo-electron microscopy and is advising on experimental design and data analysis as well as providing training.
Impact The student has generated recombinant viruses expressing fluorescently-tagged proteins to allow direct visualisation of replication organelle membranes. These have been validated and cryo-electron microscopy experiments are planned shortly.
Start Year 2020
 
Description Dr. Manman Dai 
Organisation South China Agricultural University
Country China 
Sector Academic/University 
PI Contribution Provided knowledge and expertise on avian disease research
Collaborator Contribution Provided knowledge and expertise on avian disease research
Impact Two joint publications have been generated: 1. Dai M, Zhu S, An Z, You B, Li Z, Yao Y, Nair V, Liao M. Dissection of key factors correlating with H5N1 avian influenza virus driven inflammatory lung injury of chicken identified by single-cell analysis. PLoS Pathog. 2023 Oct 11;19(10):e1011685. doi: 10.1371/journal.ppat.1011685. eCollection 2023 Oct. 2. Dai M, Sun H, Zhao L, Wu Q, You B, Xu F, Liao J, Zhu S, Li Z, Yao Y, Nair V, Liao M. Duck CD8 + T Cell Response to H5N1 Highly Pathogenic Avian Influenza Virus Infection In Vivo and In Vitro. J Immunol. 2022 Aug 8;ji2101147. doi: 10.4049/jimmunol.2101147.
Start Year 2022
 
Description Effects of co-infections on Marek's disease in poultry, and development of novel recombinant Marek's disease virus vector vaccines 
Organisation Moy Park
Country United Kingdom 
Sector Private 
PI Contribution This is a joint BBSRC funded project on investigation "Effects of co-infections on Marek's disease in poultry, and development of novel recombinant Marek's disease virus vector vaccines" between Pirbright, Moy Park and Slate Hall Veterinary Practice. Pirbright team works on the detailed investigation of prevalence of naturally occurring MDV-2 (Marek's disease virus serotype 2) infection in the field in UK; isolation and characterisation of MDV-2 field strains and development of novel MDV-2-based recombinant vaccine MDV2-IBD-ND which will protect chickens against Marek's disease, infectious bursal disease and Newcastle disease.
Collaborator Contribution Moy Park will collect the field samples and investigate the effect of MDV-2 on flock productivity and disease.
Impact Too early
Start Year 2023
 
Description Finn Frey 
Organisation University of Edinburgh
Country United Kingdom 
Sector Academic/University 
PI Contribution Worked together to identify the host factors involved in MDV latency
Collaborator Contribution Provided chicken gRNA library for screening of MDV cell line
Impact Not yet
Start Year 2018
 
Description Finn Grey - CRISPR and ISG screens 
Organisation University of Edinburgh
Department The Roslin Institute
Country United Kingdom 
Sector Academic/University 
PI Contribution My research team is providing expertise in coronavirus biology and regulation of the host cell environment. Laboratory experiments are being performed by my team.
Collaborator Contribution Finn's team have provided reagents and are advising on experimental design and data analysis.
Impact Cas-9 expressing avian cells have been generated and validated. Optimisation of the CRISPR screen is underway.
Start Year 2021
 
Description Imperial - Johnston 
Organisation Imperial College London
Department Faculty of Medicine
Country United Kingdom 
Sector Academic/University 
PI Contribution Collaboration on human rhinovirus experimental vaccines Co-Investigator on MRC project grant
Collaborator Contribution Collaboration on human rhinovirus experimental vaccines Co-Investigator on MRC project grant
Impact MRC project grant
Start Year 2017
 
Description Joint PhD project on "Exploring ultrastructural features of the virus-host interactions of MDV" 
Organisation Diamond Light Source
Country United Kingdom 
Sector Private 
PI Contribution Pirbright team provided expertise on MDV biology part of the project.
Collaborator Contribution Diamond team provided expertise on using the state-of-the-art tools to gain structural biology insights into the distinct features of MDV-host cell interactions.
Impact No outputs yet.
Start Year 2022
 
Description Joint PhD project on "Using Marek's Disease Virus to understand the RNA world of alphaherpesviruses" 
Organisation University of Surrey
Country United Kingdom 
Sector Academic/University 
PI Contribution Surrey team has provided expertise on characterization of Marek's disease virus encoded vhs protein function in vitro.
Collaborator Contribution Pirbright team has provided expertise in making the mutant virus of vhs deleted MDV and study the role of vhs in the context of infection in vitro and in vivo.
Impact The student Sophie Cutts has abstract accepted for oral presentation in both Microbiology Society Annual Conference 2024 to be held in Edinburgh 8-11 April and 14th International Symposium on Marek's Disease and Avian Herpesvirus to be held at St. Louis, USA from 12th-14th July, 2024.
Start Year 2022
 
Description Maria Harkiolaki - soft xray tomography 
Organisation Diamond Light Source
Country United Kingdom 
Sector Private 
PI Contribution The project aims to characterise coronavirus replication organelles in whole infected cells. My team provides significant experience in coronavirus replication organelles. The project is funded by a joint studentship and the student is, predominantly based in my laboratory.
Collaborator Contribution Maria's team provide extensive expertise in soft Xray tomography and are advising on experimental design and dat aanalysis.
Impact Preliminary experiments have been performed to visualise replication organelle membranes. Optimisation of experimental set up is underway.
Start Year 2020
 
Description Martin Turner - The role of cellular RNA binding proteins in Coronavirus replication 
Organisation Babraham Institute
Country United Kingdom 
Sector Academic/University 
PI Contribution My lab has begun to establish the use of a CRISPR library targeting cellular RNA binding proteins. This includes selecting the most appropriate cells, virus and conditions for the screen and the readout method and this is based on our expertise and experience in coronavirus biology. All experimental work for the collaboration has been performed in my laboratory.
Collaborator Contribution Martin Turner has provided a CRISPR knockout library targeting human and murine RNA binding proteins. He has provided advice and protocols to allow us to establish the screen using human or murine coronaviruses and will advise on analysis of data following the screen.
Impact Experiments are still in initial set up stages and no results have been generated to date.
Start Year 2020
 
Description Oxford - Strubi 
Organisation University of Oxford
Country United Kingdom 
Sector Academic/University 
PI Contribution Collaboration on virus structural biology and joint PhD studentship
Collaborator Contribution Collaboration on virus structural biology and joint PhD studentship
Impact Collaboration on virus structural biology has produced a number of novel virus structures and new understanding of virus entry and packaging
Start Year 2008
 
Description Protecting Bees collaboration 
Organisation University of Liverpool
Department Faculty of Health and Life Sciences
Country United Kingdom 
Sector Academic/University 
PI Contribution Collaboration on bee cell culture and effect of Wolbachia infection on susceptibility of cells to bee viruses
Collaborator Contribution Collaboration on bee cell culture and effect of Wolbachia infection on susceptibility of cells to bee viruses
Impact Collaboration on bee cell culture and effect of Wolbachia infection on susceptibility of cells to bee viruses
Start Year 2022
 
Description Protecting Bees collaboration 
Organisation University of York
Department Department of Biology
Country United Kingdom 
Sector Academic/University 
PI Contribution Collaboration on bee cell culture and effect of Wolbachia infection on susceptibility of cells to bee viruses
Collaborator Contribution Collaboration on bee cell culture and effect of Wolbachia infection on susceptibility of cells to bee viruses
Impact Collaboration on bee cell culture and effect of Wolbachia infection on susceptibility of cells to bee viruses
Start Year 2022
 
Description Stathis Giotis - Bat immunity and differential gene expression 
Organisation University of Essex
Country United Kingdom 
Sector Academic/University 
PI Contribution The project aims to understand the role of viral regulation of innate immune signalling in coronavirus cross-species transmission, My research team provides expertise in coronavirus biology and host cell interaction, including reverse genetics. Laboratory experiments associated with this project are conducted by my team.
Collaborator Contribution Stathis has provided bat cells and bat interferon reporter plasmids, as well as advice and expertise, to perform experiments. He will also perform data analysis of planned experiments to interrogate differential gene expression of cells infected with different viruses.
Impact To early in the project to say.
Start Year 2020
 
Description Understanding the genetic resistant to Marek's disease 
Organisation University of Surrey
Country United Kingdom 
Sector Academic/University 
PI Contribution We have provided the sequencing data of resistant and susceptible inbred chicken lines and crosses to be analyzed using AI for understanding the genetic resistant to Marek's disease.
Collaborator Contribution The collaborators contribution is to use AI for analysis to explore the determinants associated with the distinct resistance phenotype.
Impact No output yet.
Start Year 2023
 
Description Careers event at Bishop David Brown School, Woking - MB 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact The event involved discussing a variety of careers including potential STEM jobs and wider opportunities at Pirbright with students in years 7-11.
Year(s) Of Engagement Activity 2023
 
Description Interview skills workshop, Ash Manor School - MB 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact The event involved coaching year 11 students in how to structure and conduct successful interviews. Interviewing them and following up with awareness of recruitment strategy to aid their skill development. Providing feedback to candidates after reading through their CV and application letters.
Year(s) Of Engagement Activity 2024
 
Description Mental Health First Aid and Mind - Time to Talk Day 2024 (Pirbright) 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact Organised a stand that introduced the work of the Mental Health First Aiders at the Pirbright Institute in connection with the Mind - Time to Talk Day 2024.
Year(s) Of Engagement Activity 2024
URL https://www.mind.org.uk/get-involved/time-to-talk-day-2024/
 
Description Microbiology Society Annual Conference (Birmingham) - Poster presentation: Assessing the effect of inter-isolate variation on the function of RVFV virulence determinants. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Poster presentation at an international conference. The event included networking opportunities.
Year(s) Of Engagement Activity 2023
 
Description Microbiology Society Annual Conference (Birmingham) - Poster presentation: Juvenile Hormone Analogue Enhances Rift Valley fever virus Infectivity in Aedes aegypti Aag2 Cells. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Poster presentation at an international conference. The event included networking opportunities.
Year(s) Of Engagement Activity 2023
 
Description Oral presentation at 13th International Veterinary Immunology Symposium 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Project results presented to a highly interested and engaging audience of more than 200 scientists and students.
Year(s) Of Engagement Activity 2023
 
Description PRIDE day event TPI - HM, ND, CH, YC, MB 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact Stall presented describing recent research of the Coronavirus Cellular Biology group at the Pirbright Institute PRIDE event, celebrating Pirbright science and activites.
Year(s) Of Engagement Activity 2023
 
Description Pirbright Family Day (Pirbright) 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Organised and presented at stands that displayed science activities in the research group, the high containment community and within science services (CCG CL3 Virology; insectaries). Informed public about science undertaken at the Institute.
Year(s) Of Engagement Activity 2023
 
Description Pirbright village fair 2023 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact This annual event, organised by Pirbright Parish Council and St. Michael & All Angels Church and hosted on the village green, offered an ideal occasion for the Institute to foster connections with the local community and ignite their interest in cutting-edge scientific research.

This year's theme was 'Around the World' and included stalls and activities to reflect as many different countries and cultures as possible.

The Institute's stall featured various engaging activities. Attendees, young and old had the opportunity to shake and reassemble virus models, swab Henrietta the hen to determine her health status, observe Culicoides nubeculosus midges in a display net, and examine midge larvae and pupae under a microscope for a closer inspection.
Year(s) Of Engagement Activity 2023
URL https://thebull.pirbright.ac.uk/Interact/Pages/Content/Document.aspx?id=7311&SearchId=208907
 
Description Poster and flash presentation at Microscience Microscopy Congress - PP 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Other audiences
Results and Impact 3 minute flask presentation and poster entitled "Correlative cryo-bioimaging to study coronavirus replication organelles"
Year(s) Of Engagement Activity 2023
 
Description Poster presentation at International Nidovirus Syposium 2023 - HM 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact Poster entitled "Cross virus comparison of coronavirus proteins involved in replication organelle formation"
Year(s) Of Engagement Activity 2023
 
Description Poster presentation at Microbiology Society annual conference - YC 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Other audiences
Results and Impact Poster entitled "Development of a CRISPR/Cas9 GeCKO library screen to identify pro-viral cellular genes in chicken cells that can be exploited to control avian endemic viruses"
Year(s) Of Engagement Activity 2023
 
Description Poster presentation at The British Society for Immunology Congress 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact I presented a poster on the Pirbright immunological toolbox projects and activities, contibuting and adavancing the veterinary immunlogy.
Year(s) Of Engagement Activity 2023
 
Description Presentation at International Nidovirus Symposium 2023 - PP 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact 10 minute presentation entitled "Correlative cryo-bioimaging to study coronavirus replication organelles"
Year(s) Of Engagement Activity 2023
 
Description Presentation at Microbiology Society annual conference - PP 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Other audiences
Results and Impact 10 minute talk entitled "Correlative cryo-bioimaging to study coronavirus replication organelles"
Year(s) Of Engagement Activity 2023
 
Description Presentation at Microscience Microscopy Congress - HM 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Other audiences
Results and Impact Poster entitled "Cross virus comparison of coronavirus proteins involved in replication organelle formation"
Year(s) Of Engagement Activity 2023
 
Description Presentation at UK-ICN ECR meeting - PP 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Postgraduate students
Results and Impact 10 minute talk entitled "Structural studies of coronavirus replication organelles".
Year(s) Of Engagement Activity 2024
 
Description RIVR (Recently Independent Virology Researchers) Annual Meeting (Liverpool) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Presented a science talk to other researcher on a national level. The event included networking activities. Future collaborations have been planned.
Year(s) Of Engagement Activity 2024
 
Description Southern England Virology Network meeting (Animal and Plant Health Agency, Weybridge) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Professional Practitioners
Results and Impact Presented a science talk to researchers from research organisations (government and academia) in South England. The event also included networking activities. Future meetings have been organised.
Year(s) Of Engagement Activity 2023
 
Description Talk on stomoxys calcitrans colony 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact Talk given to meeting of Public Health Entomology Group on stomoxys calcitrans at LSHTM (Melanie Nicholls)
Year(s) Of Engagement Activity 2023
 
Description participation in Pirbright celebration day 2023 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Participated in bioinformatics and sequencing stall
Year(s) Of Engagement Activity 2023