BBSRC National Bioscience Research Infrastructure: Low Containment Services and Science Platforms
Lead Research Organisation:
The Pirbright Institute
Department Name: UNLISTED
Abstract
Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.
Technical Summary
The purpose of the Low Containment Services and Science Technology Platforms capability is to both support the High Containment Services and Science Technology Platforms (PIR-NBRI-1) and to provide dedicated facilities for low containment study of virus: host interactions. The NBRI includes five major pieces of infrastructure: The BBSRC National Vaccinology Centre: The Jenner Building two insectary facilities (The Philip Mellor Insectary and the IS4L facility) The Houghton Facility a poultry hatchery unit and The Biggs Building a low containment facility for infection of poultry. The Jenner building houses around 100 scientists in low containment laboratories and offices and includes those studying poultry viruses that are endemic in the UK (including low pathogenicity animal influenza viruses Marek’s disease virus and infectious bronchitis viruses) and scientists performing fundamental host-pathogen interaction studies including those developing genetically modified insect lines. Jenner also supports scientists who primarily work in high containment (under our PIR-NBRI-1 application) who require low containment space for some tasks and bioinformaticians. The other four infrastructures provide dedicated specialist services for production maintenance and infection of poultry and insects. The NBRI supports large and diverse teams of workers in engineering risk and assurance and animal services that rigorously maintain the highest standards in running of low containment facilities requiring highly specialised controlled temperature regimes and animal husbandry.
A range of science platforms are also maintained in Jenner as part of the NBRI. The Immunological Toolbox facility provides a diverse range of unique reagents and resources to promote veterinary immunology and vaccinology research both in the UK and globally. In addition bioimaging flow cytometry and bioinformatics services are provided that allow comparable data for studies carried out across containment levels and harmonisation in analysis and interpretation. Together these science platforms not only provide vital collaborative support during studies but also provide training to a wide range of users helping to sustain the UK’s excellence in bioscience.
A range of science platforms are also maintained in Jenner as part of the NBRI. The Immunological Toolbox facility provides a diverse range of unique reagents and resources to promote veterinary immunology and vaccinology research both in the UK and globally. In addition bioimaging flow cytometry and bioinformatics services are provided that allow comparable data for studies carried out across containment levels and harmonisation in analysis and interpretation. Together these science platforms not only provide vital collaborative support during studies but also provide training to a wide range of users helping to sustain the UK’s excellence in bioscience.
Planned Impact
unavailable
Organisations
- The Pirbright Institute (Lead Research Organisation)
- Babraham Institute (Collaboration)
- University of Surrey (Collaboration)
- University of York (Collaboration)
- DIAMOND LIGHT SOURCE (Collaboration)
- UNIVERSITY OF CAMBRIDGE (Collaboration)
- IMPERIAL COLLEGE LONDON (Collaboration)
- UNIVERSITY OF OXFORD (Collaboration)
- UNIVERSITY OF EDINBURGH (Collaboration)
- U.S. Department of Agriculture USDA (Collaboration)
- Washington State University (Collaboration)
- Moy Park (Collaboration)
- South China Agricultural University (Collaboration)
- UNIVERSITY OF LIVERPOOL (Collaboration)
- UNIVERSITY OF ESSEX (Collaboration)
- University of Bristol (Collaboration)
Publications

Bilotti K
(2024)
One-pot Golden Gate Assembly of an avian infectious bronchitis virus reverse genetics system
in PLOS ONE


Doyle N
(2023)
Antibody-labelling correlative light electron microscopy

Doyle N
(2024)
A novel optimized pre-embedding antibody-labelling correlative light electron microscopy technique
in Access Microbiology


Maccari G
(2024)
The 2024 IPD-MHC database update: a comprehensive resource for major histocompatibility complex studies.
in Nucleic acids research

Maringer K
(2023)
Re-evaluating the mosquito RNAi pathway's influence on arbovirus transmission.
in Trends in parasitology


Xu M
(2023)
3'UTR of ALV-J can affect viral replication through promoting transcription and mRNA nuclear export.
in Journal of virology
Description | Imperial College London (Masters-level training) |
Geographic Reach | Multiple continents/international |
Policy Influence Type | Influenced training of practitioners or researchers |
Impact | The students will be better prepared for how to prepare and respond to outbreaks of tropical vector-borne diseases, which is highly relevant as many students on the course were from disease-endemic countries. |
URL | https://www.imperial.ac.uk |
Description | University of Oxford (Medical students training) |
Geographic Reach | National |
Policy Influence Type | Influenced training of practitioners or researchers |
Impact | Medical students in the UK may encounter tropical diseases in patients returning from overseas travel. In this lecture they gained an insight into the disease caused by dengue virus and how people can protect themselves when they travel overseas, allowing them to better care for patients when they enter the clinic. |
URL | https://www.ox.ac.uk |
Description | Zika pandemic preparedness ten-year plan consultation (Wellcome Trust/WHO) |
Geographic Reach | Multiple continents/international |
Policy Influence Type | Participation in a guidance/advisory committee |
Description | Application of molecular xenomonitoring in a lateral flow test format for multiplexed monitoring of vector-borne viruses and their animal reservoirs and insect vectors in low-income settings |
Amount | £49,015 (GBP) |
Funding ID | IAA2149 |
Organisation | Medical Research Council (MRC) |
Sector | Public |
Country | United Kingdom |
Start | 03/2024 |
End | 03/2025 |
Description | Blood Markers for prediction of respiratory virus infection |
Amount | £21,000 (GBP) |
Organisation | The Pirbright Institute |
Sector | Academic/University |
Country | United Kingdom |
Start | 05/2023 |
End | 07/2024 |
Description | Development of CVI988 based recombinant vaccine |
Amount | £238,000 (GBP) |
Organisation | Eco Animal Health Ltd |
Sector | Private |
Country | United Kingdom |
Start | 03/2023 |
End | 02/2024 |
Description | Development of avian herpesvirus vector vaccines for poultry |
Amount | £772,000 (GBP) |
Organisation | MSD Animal Health |
Sector | Private |
Country | United Kingdom |
Start | 01/2024 |
End | 12/2026 |
Description | Effects of co-infections on Marek's disease in poultry and development of novel recombinant Marek's disease virus vector vaccines |
Amount | £562,000 (GBP) |
Funding ID | BB/X017575/1 |
Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
Sector | Public |
Country | United Kingdom |
Start | 09/2023 |
End | 09/2026 |
Description | Emerging porcine influenza and coronaviruses" (EPICVIR) |
Amount | £596,027 (GBP) |
Funding ID | BB/X019780/1 |
Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
Sector | Public |
Country | United Kingdom |
Start | 11/2023 |
End | 10/2026 |
Description | MRC Impact Accelerator Accounts (Tropical Infectious Disease Consortium: Expanding and Accelerating Product Development) |
Amount | £53,362 (GBP) |
Funding ID | IAA2148 |
Organisation | Medical Research Council (MRC) |
Sector | Public |
Country | United Kingdom |
Start | 03/2024 |
End | 02/2025 |
Description | Travel Grant |
Amount | £350 (GBP) |
Organisation | Microbiology Society |
Sector | Learned Society |
Country | United Kingdom |
Start | 03/2024 |
End | 04/2024 |
Description | Using machine learning to identify the molecular determinants of vector-borne transmission in viruses |
Amount | £110,327 (GBP) |
Organisation | The Pirbright Institute |
Sector | Academic/University |
Country | United Kingdom |
Start | 09/2024 |
End | 04/2028 |
Title | Development of recomibinant system to express pig IgM and IgA |
Description | This system allows the recombinant expression oligomeric antibodies (IgM & IgA) from pig. |
Type Of Material | Technology assay or reagent |
Year Produced | 2023 |
Provided To Others? | No |
Impact | No impact yet |
Title | Development of recomibinant system to express rabbit IgG |
Description | This system has been developed to express recombinant IgG from rabbit B cells |
Type Of Material | Technology assay or reagent |
Year Produced | 2024 |
Provided To Others? | Yes |
Impact | No impact yet |
Title | Establishment of an efficient CRISPR/Cas9 gRNA library screening to identify the host factors required for maintaining the transformed phenotype of MDV tumour cell lines |
Description | Herpesviruses are large dsDNA viruses that cause widespread, lifelong latent infections in different hosts, through multiple virus-host interactions to create a delicate balance between the virus and the host. CRISPR/Cas9-based gene editing is emerging as a powerful tool to investigate the precise determinants of latency in a number of herpesvirus infections. Marek's disease virus (MDV-1) is a lymphotropic a-herpesvirus associated with latent infections and malignant CD4+ T-cell lymphomas in chicken. MDV-1 has a two-phase life cycle, consisting of a lytic and a latent phase, the latter closely associated with the oncogenesis of the virus, yet the underlying molecular mechanisms of cell transformation remain unclear. Better understanding of the factors that maintain the latency of the virus will provide insights into novel intervention strategies to interfere with the neoplastic process. We have used a high throughput genome-wide CRISPR/Cas9 gene knockout strategy, combined with next generation sequencing to identify the genes critical for maintenance of the transformed phenotype. 339 host genes have been identified being essential for maintaining the transformed phenotype of MDV cell line. |
Type Of Material | Technology assay or reagent |
Year Produced | 2023 |
Provided To Others? | No |
Impact | Establishment of the pipeline of CRISPR library screening in chicken cell lines has given the opportunity for the identification factors associated with disease pathogenesis and neoplastic transformation. The identified genes could serve as targets for inducing de novo genetic resistance using gene editing approaches. |
Title | Establishment of an efficient CRISPR/Cas9-based screening pipeline for herpesviruses to study the gene function and streamline the vector platform for recombinant vaccine development |
Description | The development of both cosmid DNA and Bacterial artificial chromosome (BAC) technologies has greatly facilitated the introduction of mutations into the viral genomes of herpesviruses to study gene functions. However, cloning of viral genomes as BAC plasmid and subsequent mutagenesis is inefficient, time-consuming and may introduce mutations by repeated passages. Following our success in efficient CRISPR/Cas9 editing of the MDV genome in both replicating virus and MDV transformed cell lines and of HVT genome for the recombinant vaccine development, we have developed an efficient CRISPR/Cas9-based screening pipeline for herpesviruses to identify the essential/non-essential genes for study of the gene function and streamline the vector platform for recombinant vaccine development. |
Type Of Material | Technology assay or reagent |
Year Produced | 2022 |
Provided To Others? | No |
Impact | Identification of essential and non-essential genes of herpesviruses provided opportunities to study gene function in vitro and in vivo. Knocking out the non-essential genes in the HVT vector can enhance HVT replication in vivo enabling recombinant HVT-based vaccines to induce stronger immune responses. |
Title | Lateral flow detection of avian leukosis viruses and Reticuloendotheliosis virus |
Description | The system for detecting ALV/REV based on CRISPR-Cas13a, a SHERLOCK reaction system consists of specific RPA primers for amplifying target nucleic acid fragments, Cas13a protein, crRNA, and Lateral flow chromatography test strip for visualized display of results. The assay started with pre-amplification of either a DNA or RNA target input. Amplified targets are then converted to RNA via T7 transcription and detected by Cas13-crRNA complexes, which activate and cleave fluorescent RNA reporters based on its collateral cleavage nature. The result can be visualized using colorimetric lateral flow reaction. The test is rapid, sensitive and specific for detection of ALV/REV at 37°C. The product can commercialized with a great clinical application prospects. |
Type Of Material | Technology assay or reagent |
Year Produced | 2020 |
Provided To Others? | No |
Impact | The early identi?cation and removal of virus-shedding birds are essential to reduce the transmission. This rapid, simple-to-use and cost effective on-site diagnostic method will aid the eradication of ALV/REV in the affected area. |
Title | The establishment of an efficient pipeline for in situ CRISPR editing of the Marek's disease virus (MDV) genome in lymphoma-derived cell lines |
Description | The lymphoblastoid cell lines (LCLs) derived from MD lymphomas have served as valuable resources to study virus-host molecular interactions in transformed cells. However, detailed investigations into the functional role of different viral and host determinants in these cells have been difficult due to the lack of tools for in situ manipulation of viral/host genomes in MDV-transformed cell lines. Our recent success in efficient CRISPR/Cas9 editing of the MDV genome in LCLs has demonstrated the potential for targeted editing to dissect the regulatory pathways involved in latency, transformation, reactivation and lytic switch. |
Type Of Material | Technology assay or reagent |
Year Produced | 2018 |
Provided To Others? | Yes |
Impact | Using this approach, we have demonstrated viral genes such as pp38 and MDV-miR-M4 are not essential for maintaining the transformed phenotype. On the other hand, deletion of MDV-encoded oncogene Meq or mutations preventing its interactions resulted in cell death demonstrating the essential role of Meq in transformation. |
URL | https://www.mdpi.com/1999-4915/10/6/279 |
Title | scRNA seq analysis of porcine BAL |
Description | We describe for the first time scRNA-seq analysis of porcine bronchoalveolar lavage (BAL), a cell source increasingly used to analyse respiratory immune responses, and which has been shown to be major correlate for protection against respiratory infections such as influenza, respiratory syncytial virus, and SARS-CoV-2. Our work reveals both similar and unique cell subsets and divergent transcriptome profiles of BAL immune cells compared to publicly available data from blood cells. |
Type Of Material | Technology assay or reagent |
Year Produced | 2024 |
Provided To Others? | Yes |
Impact | The data we have generated will provide an atlas for future pig BAL scRNA-seq studies. |
Title | Spatial transcriptomics of Chicken trachea infected with Infectious bronchitis virus to identify transcriptomic signatures |
Description | Rhode Island Red SPF chickens were infected with M41-CK (a cell culture adapted pathogenic IBV virus) to investogate the evolution of the virus in chickens after infection, and compare with live attenuated vaccine versions of the same virus. Illumina sequence datasets were piped into the 10X proprietary analysis pipeline 'Space Ranger' for reads alignments, feature barcode matrices generation and secondary analysis including clustering and differential gene expression. Outputs from Space Ranger consisting of tissue images, tissue positions and feature barcode matrix were further piped into previously published R-based spatial pipeline 'Giotto' (Dries et al, 2021) for detailed analysis such as dimensionality reduction, co-visualization of gene expression and spatial location, cell type marker gene detection, spatial structure analysis, spatial gene co-expression patterns, etc. |
Type Of Material | Database/Collection of data |
Year Produced | 2024 |
Provided To Others? | No |
Impact | The dataset will have impact for other research groups investigating avian coronavirus vaccines. They will also have a broader impact and be of interest to wider coronavirus vaccine development and research thorough the information on vaccine evolution and host response. The datasets will be of interest to persons within the field of spatial biology field, notably the software developers and bioinformaticians, as a dataset to test new models and algorithms. The results will be of interest specifically for those in industry who are involved in developing novel and more rational strategies for developing vaccines. |
Description | Andrew Davidson - Proteome of viral replication organelles |
Organisation | University of Bristol |
Country | United Kingdom |
Sector | Academic/University |
PI Contribution | Projects are being initiated to study the cellular proteins associated with coronavirus replication organelle membranes and a funding application has been submitted to BBSRC. |
Collaborator Contribution | Andrew has provided assistance with experimental planning and drafting of a grant application. He has also provided protocols to allow preliminary data to be generated. Once protocols have been established, Andrew will facilitate mass spectrometry data generation and will analyse the resulting data. |
Impact | No outcomes to date, projects being initiated and funding applications in progress. |
Start Year | 2018 |
Description | Cambridge |
Organisation | University of Cambridge |
Country | United Kingdom |
Sector | Academic/University |
PI Contribution | Collaborative research on epitope display systems and use of functional genetic screens. |
Collaborator Contribution | Collaborative research on epitope display systems and use of functional genetic screens. |
Impact | Collaborative research on epitope display systems and use of functional genetic screens. |
Start Year | 2022 |
Description | Collaboration with USDA to seuence anti-pig immune protein hybridomas |
Organisation | U.S. Department of Agriculture USDA |
Country | United States |
Sector | Public |
PI Contribution | Sequenced 14 mouse anti-pig immune protein hybridomas. |
Collaborator Contribution | Shared hybridomas for sequencing and agreed to post availbility of these recombinant antibodies on immunological toolbox website |
Impact | Preservation of important immune reagents as sequences, which will also faciliate sharing of these reagents. |
Start Year | 2023 |
Description | Collaboration with WSU to seuence anti-bovine immune protein hybridomas |
Organisation | Washington State University |
Country | United States |
Sector | Academic/University |
PI Contribution | Sequenced 15 mouse anti-bovine immune protein hybridomas. |
Collaborator Contribution | Shared hybridomas for sequenceing and agree to post availiity of these reagents as recombinant on the immunological toolbox website for wider community |
Impact | Permanently secured these hynrdiomas as antibody sequences |
Start Year | 2023 |
Description | Dan Clare - cryo-electron microscopy |
Organisation | Diamond Light Source |
Country | United Kingdom |
Sector | Private |
PI Contribution | The project aims to characterise proteins complexes associated with coronavirus replication organelles. My team provides significant expertise in replication organelles induced by coronaviruses. Laboratory work is performed by my team. The project is funded by a joint PhD studentship working, predominantly in my laboratory. |
Collaborator Contribution | Dan provides expertise in cryo-electron microscopy and is advising on experimental design and data analysis as well as providing training. |
Impact | The student has generated recombinant viruses expressing fluorescently-tagged proteins to allow direct visualisation of replication organelle membranes. These have been validated and cryo-electron microscopy experiments are planned shortly. |
Start Year | 2020 |
Description | Dr. Manman Dai |
Organisation | South China Agricultural University |
Country | China |
Sector | Academic/University |
PI Contribution | Provided knowledge and expertise on avian disease research |
Collaborator Contribution | Provided knowledge and expertise on avian disease research |
Impact | Two joint publications have been generated: 1. Dai M, Zhu S, An Z, You B, Li Z, Yao Y, Nair V, Liao M. Dissection of key factors correlating with H5N1 avian influenza virus driven inflammatory lung injury of chicken identified by single-cell analysis. PLoS Pathog. 2023 Oct 11;19(10):e1011685. doi: 10.1371/journal.ppat.1011685. eCollection 2023 Oct. 2. Dai M, Sun H, Zhao L, Wu Q, You B, Xu F, Liao J, Zhu S, Li Z, Yao Y, Nair V, Liao M. Duck CD8 + T Cell Response to H5N1 Highly Pathogenic Avian Influenza Virus Infection In Vivo and In Vitro. J Immunol. 2022 Aug 8;ji2101147. doi: 10.4049/jimmunol.2101147. |
Start Year | 2022 |
Description | Effects of co-infections on Marek's disease in poultry, and development of novel recombinant Marek's disease virus vector vaccines |
Organisation | Moy Park |
Country | United Kingdom |
Sector | Private |
PI Contribution | This is a joint BBSRC funded project on investigation "Effects of co-infections on Marek's disease in poultry, and development of novel recombinant Marek's disease virus vector vaccines" between Pirbright, Moy Park and Slate Hall Veterinary Practice. Pirbright team works on the detailed investigation of prevalence of naturally occurring MDV-2 (Marek's disease virus serotype 2) infection in the field in UK; isolation and characterisation of MDV-2 field strains and development of novel MDV-2-based recombinant vaccine MDV2-IBD-ND which will protect chickens against Marek's disease, infectious bursal disease and Newcastle disease. |
Collaborator Contribution | Moy Park will collect the field samples and investigate the effect of MDV-2 on flock productivity and disease. |
Impact | Too early |
Start Year | 2023 |
Description | Finn Frey |
Organisation | University of Edinburgh |
Country | United Kingdom |
Sector | Academic/University |
PI Contribution | Worked together to identify the host factors involved in MDV latency |
Collaborator Contribution | Provided chicken gRNA library for screening of MDV cell line |
Impact | Not yet |
Start Year | 2018 |
Description | Finn Grey - CRISPR and ISG screens |
Organisation | University of Edinburgh |
Department | The Roslin Institute |
Country | United Kingdom |
Sector | Academic/University |
PI Contribution | My research team is providing expertise in coronavirus biology and regulation of the host cell environment. Laboratory experiments are being performed by my team. |
Collaborator Contribution | Finn's team have provided reagents and are advising on experimental design and data analysis. |
Impact | Cas-9 expressing avian cells have been generated and validated. Optimisation of the CRISPR screen is underway. |
Start Year | 2021 |
Description | Imperial - Johnston |
Organisation | Imperial College London |
Department | Faculty of Medicine |
Country | United Kingdom |
Sector | Academic/University |
PI Contribution | Collaboration on human rhinovirus experimental vaccines Co-Investigator on MRC project grant |
Collaborator Contribution | Collaboration on human rhinovirus experimental vaccines Co-Investigator on MRC project grant |
Impact | MRC project grant |
Start Year | 2017 |
Description | Joint PhD project on "Exploring ultrastructural features of the virus-host interactions of MDV" |
Organisation | Diamond Light Source |
Country | United Kingdom |
Sector | Private |
PI Contribution | Pirbright team provided expertise on MDV biology part of the project. |
Collaborator Contribution | Diamond team provided expertise on using the state-of-the-art tools to gain structural biology insights into the distinct features of MDV-host cell interactions. |
Impact | No outputs yet. |
Start Year | 2022 |
Description | Joint PhD project on "Using Marek's Disease Virus to understand the RNA world of alphaherpesviruses" |
Organisation | University of Surrey |
Country | United Kingdom |
Sector | Academic/University |
PI Contribution | Surrey team has provided expertise on characterization of Marek's disease virus encoded vhs protein function in vitro. |
Collaborator Contribution | Pirbright team has provided expertise in making the mutant virus of vhs deleted MDV and study the role of vhs in the context of infection in vitro and in vivo. |
Impact | The student Sophie Cutts has abstract accepted for oral presentation in both Microbiology Society Annual Conference 2024 to be held in Edinburgh 8-11 April and 14th International Symposium on Marek's Disease and Avian Herpesvirus to be held at St. Louis, USA from 12th-14th July, 2024. |
Start Year | 2022 |
Description | Maria Harkiolaki - soft xray tomography |
Organisation | Diamond Light Source |
Country | United Kingdom |
Sector | Private |
PI Contribution | The project aims to characterise coronavirus replication organelles in whole infected cells. My team provides significant experience in coronavirus replication organelles. The project is funded by a joint studentship and the student is, predominantly based in my laboratory. |
Collaborator Contribution | Maria's team provide extensive expertise in soft Xray tomography and are advising on experimental design and dat aanalysis. |
Impact | Preliminary experiments have been performed to visualise replication organelle membranes. Optimisation of experimental set up is underway. |
Start Year | 2020 |
Description | Martin Turner - The role of cellular RNA binding proteins in Coronavirus replication |
Organisation | Babraham Institute |
Country | United Kingdom |
Sector | Academic/University |
PI Contribution | My lab has begun to establish the use of a CRISPR library targeting cellular RNA binding proteins. This includes selecting the most appropriate cells, virus and conditions for the screen and the readout method and this is based on our expertise and experience in coronavirus biology. All experimental work for the collaboration has been performed in my laboratory. |
Collaborator Contribution | Martin Turner has provided a CRISPR knockout library targeting human and murine RNA binding proteins. He has provided advice and protocols to allow us to establish the screen using human or murine coronaviruses and will advise on analysis of data following the screen. |
Impact | Experiments are still in initial set up stages and no results have been generated to date. |
Start Year | 2020 |
Description | Oxford - Strubi |
Organisation | University of Oxford |
Country | United Kingdom |
Sector | Academic/University |
PI Contribution | Collaboration on virus structural biology and joint PhD studentship |
Collaborator Contribution | Collaboration on virus structural biology and joint PhD studentship |
Impact | Collaboration on virus structural biology has produced a number of novel virus structures and new understanding of virus entry and packaging |
Start Year | 2008 |
Description | Protecting Bees collaboration |
Organisation | University of Liverpool |
Department | Faculty of Health and Life Sciences |
Country | United Kingdom |
Sector | Academic/University |
PI Contribution | Collaboration on bee cell culture and effect of Wolbachia infection on susceptibility of cells to bee viruses |
Collaborator Contribution | Collaboration on bee cell culture and effect of Wolbachia infection on susceptibility of cells to bee viruses |
Impact | Collaboration on bee cell culture and effect of Wolbachia infection on susceptibility of cells to bee viruses |
Start Year | 2022 |
Description | Protecting Bees collaboration |
Organisation | University of York |
Department | Department of Biology |
Country | United Kingdom |
Sector | Academic/University |
PI Contribution | Collaboration on bee cell culture and effect of Wolbachia infection on susceptibility of cells to bee viruses |
Collaborator Contribution | Collaboration on bee cell culture and effect of Wolbachia infection on susceptibility of cells to bee viruses |
Impact | Collaboration on bee cell culture and effect of Wolbachia infection on susceptibility of cells to bee viruses |
Start Year | 2022 |
Description | Stathis Giotis - Bat immunity and differential gene expression |
Organisation | University of Essex |
Country | United Kingdom |
Sector | Academic/University |
PI Contribution | The project aims to understand the role of viral regulation of innate immune signalling in coronavirus cross-species transmission, My research team provides expertise in coronavirus biology and host cell interaction, including reverse genetics. Laboratory experiments associated with this project are conducted by my team. |
Collaborator Contribution | Stathis has provided bat cells and bat interferon reporter plasmids, as well as advice and expertise, to perform experiments. He will also perform data analysis of planned experiments to interrogate differential gene expression of cells infected with different viruses. |
Impact | To early in the project to say. |
Start Year | 2020 |
Description | Understanding the genetic resistant to Marek's disease |
Organisation | University of Surrey |
Country | United Kingdom |
Sector | Academic/University |
PI Contribution | We have provided the sequencing data of resistant and susceptible inbred chicken lines and crosses to be analyzed using AI for understanding the genetic resistant to Marek's disease. |
Collaborator Contribution | The collaborators contribution is to use AI for analysis to explore the determinants associated with the distinct resistance phenotype. |
Impact | No output yet. |
Start Year | 2023 |
Description | Careers event at Bishop David Brown School, Woking - MB |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Schools |
Results and Impact | The event involved discussing a variety of careers including potential STEM jobs and wider opportunities at Pirbright with students in years 7-11. |
Year(s) Of Engagement Activity | 2023 |
Description | Interview skills workshop, Ash Manor School - MB |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Schools |
Results and Impact | The event involved coaching year 11 students in how to structure and conduct successful interviews. Interviewing them and following up with awareness of recruitment strategy to aid their skill development. Providing feedback to candidates after reading through their CV and application letters. |
Year(s) Of Engagement Activity | 2024 |
Description | Mental Health First Aid and Mind - Time to Talk Day 2024 (Pirbright) |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Professional Practitioners |
Results and Impact | Organised a stand that introduced the work of the Mental Health First Aiders at the Pirbright Institute in connection with the Mind - Time to Talk Day 2024. |
Year(s) Of Engagement Activity | 2024 |
URL | https://www.mind.org.uk/get-involved/time-to-talk-day-2024/ |
Description | Microbiology Society Annual Conference (Birmingham) - Poster presentation: Assessing the effect of inter-isolate variation on the function of RVFV virulence determinants. |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | Poster presentation at an international conference. The event included networking opportunities. |
Year(s) Of Engagement Activity | 2023 |
Description | Microbiology Society Annual Conference (Birmingham) - Poster presentation: Juvenile Hormone Analogue Enhances Rift Valley fever virus Infectivity in Aedes aegypti Aag2 Cells. |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | Poster presentation at an international conference. The event included networking opportunities. |
Year(s) Of Engagement Activity | 2023 |
Description | Oral presentation at 13th International Veterinary Immunology Symposium |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | Project results presented to a highly interested and engaging audience of more than 200 scientists and students. |
Year(s) Of Engagement Activity | 2023 |
Description | PRIDE day event TPI - HM, ND, CH, YC, MB |
Form Of Engagement Activity | Participation in an open day or visit at my research institution |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Public/other audiences |
Results and Impact | Stall presented describing recent research of the Coronavirus Cellular Biology group at the Pirbright Institute PRIDE event, celebrating Pirbright science and activites. |
Year(s) Of Engagement Activity | 2023 |
Description | Pirbright Family Day (Pirbright) |
Form Of Engagement Activity | Participation in an open day or visit at my research institution |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Public/other audiences |
Results and Impact | Organised and presented at stands that displayed science activities in the research group, the high containment community and within science services (CCG CL3 Virology; insectaries). Informed public about science undertaken at the Institute. |
Year(s) Of Engagement Activity | 2023 |
Description | Pirbright village fair 2023 |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Public/other audiences |
Results and Impact | This annual event, organised by Pirbright Parish Council and St. Michael & All Angels Church and hosted on the village green, offered an ideal occasion for the Institute to foster connections with the local community and ignite their interest in cutting-edge scientific research. This year's theme was 'Around the World' and included stalls and activities to reflect as many different countries and cultures as possible. The Institute's stall featured various engaging activities. Attendees, young and old had the opportunity to shake and reassemble virus models, swab Henrietta the hen to determine her health status, observe Culicoides nubeculosus midges in a display net, and examine midge larvae and pupae under a microscope for a closer inspection. |
Year(s) Of Engagement Activity | 2023 |
URL | https://thebull.pirbright.ac.uk/Interact/Pages/Content/Document.aspx?id=7311&SearchId=208907 |
Description | Poster and flash presentation at Microscience Microscopy Congress - PP |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Other audiences |
Results and Impact | 3 minute flask presentation and poster entitled "Correlative cryo-bioimaging to study coronavirus replication organelles" |
Year(s) Of Engagement Activity | 2023 |
Description | Poster presentation at International Nidovirus Syposium 2023 - HM |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other audiences |
Results and Impact | Poster entitled "Cross virus comparison of coronavirus proteins involved in replication organelle formation" |
Year(s) Of Engagement Activity | 2023 |
Description | Poster presentation at Microbiology Society annual conference - YC |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Other audiences |
Results and Impact | Poster entitled "Development of a CRISPR/Cas9 GeCKO library screen to identify pro-viral cellular genes in chicken cells that can be exploited to control avian endemic viruses" |
Year(s) Of Engagement Activity | 2023 |
Description | Poster presentation at The British Society for Immunology Congress |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Professional Practitioners |
Results and Impact | I presented a poster on the Pirbright immunological toolbox projects and activities, contibuting and adavancing the veterinary immunlogy. |
Year(s) Of Engagement Activity | 2023 |
Description | Presentation at International Nidovirus Symposium 2023 - PP |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other audiences |
Results and Impact | 10 minute presentation entitled "Correlative cryo-bioimaging to study coronavirus replication organelles" |
Year(s) Of Engagement Activity | 2023 |
Description | Presentation at Microbiology Society annual conference - PP |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Other audiences |
Results and Impact | 10 minute talk entitled "Correlative cryo-bioimaging to study coronavirus replication organelles" |
Year(s) Of Engagement Activity | 2023 |
Description | Presentation at Microscience Microscopy Congress - HM |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Other audiences |
Results and Impact | Poster entitled "Cross virus comparison of coronavirus proteins involved in replication organelle formation" |
Year(s) Of Engagement Activity | 2023 |
Description | Presentation at UK-ICN ECR meeting - PP |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Postgraduate students |
Results and Impact | 10 minute talk entitled "Structural studies of coronavirus replication organelles". |
Year(s) Of Engagement Activity | 2024 |
Description | RIVR (Recently Independent Virology Researchers) Annual Meeting (Liverpool) |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Professional Practitioners |
Results and Impact | Presented a science talk to other researcher on a national level. The event included networking activities. Future collaborations have been planned. |
Year(s) Of Engagement Activity | 2024 |
Description | Southern England Virology Network meeting (Animal and Plant Health Agency, Weybridge) |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Professional Practitioners |
Results and Impact | Presented a science talk to researchers from research organisations (government and academia) in South England. The event also included networking activities. Future meetings have been organised. |
Year(s) Of Engagement Activity | 2023 |
Description | Talk on stomoxys calcitrans colony |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Industry/Business |
Results and Impact | Talk given to meeting of Public Health Entomology Group on stomoxys calcitrans at LSHTM (Melanie Nicholls) |
Year(s) Of Engagement Activity | 2023 |
Description | participation in Pirbright celebration day 2023 |
Form Of Engagement Activity | Participation in an open day or visit at my research institution |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Public/other audiences |
Results and Impact | Participated in bioinformatics and sequencing stall |
Year(s) Of Engagement Activity | 2023 |