Plant stress responses in the rumen

Lead Research Organisation: Aberystwyth University
Department Name: IBERS

Abstract

At least half of the plant cells ingested by ruminants are viable and capable of responding to the multiple and simultaneous stresses they are suddenly exposed to (anaerobic, 39oC, invasive microbial population). These responses culminate in autolytic cell death in both the presence and absence of micro-organisms. This work involves detailed analysis of the biochemical and gene expression response of model and forage crop plants to understand the regulation of autolysis in ingested forage in the rumen, with particular emphasis on proteolysis. This will enable the identification of plant-based metabolic and genetic control points that could be amenable to manipulation and so be exploited to alter rumen ecosystem outputs.

Publications

10 25 50

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Edwards J (2008) Adaptation to flavomycin in the ruminal bacterium, Prevotella bryantii in Journal of Applied Microbiology

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Kim E.J. (2009) Dietary transformation of lipid in the rumen microbial ecosystem in Asian-Australasian Journal of Animal Sciences

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Kingston-Smith A (2008) From plants to animals; the role of plant cell death in ruminant herbivores in Journal of Experimental Botany

 
Description Evidence of successional biology of colonisation during feed degradation. Identification of feed-related colonisation patterns, evidence of biofilm formation in very early stages post-ingestion.
Exploitation Route Underpinned current next generation detailed analysis of rumen metagenome as relating to the functional rumen.
Sectors Agriculture, Food and Drink,Education,Environment

 
Description BBSRC responsive mode Genomic selection of sheep
Amount £411,076 (GBP)
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 04/2012 
End 03/2015
 
Description DEFRA Nitrogen
Amount £1,113,737 (GBP)
Funding ID AC0122 
Organisation Department For Environment, Food And Rural Affairs (DEFRA) 
Sector Public
Country United Kingdom
Start 04/2012 
End 09/2018
 
Description EU CANTOGETHER
Amount £194,976 (GBP)
Funding ID 289328 
Organisation European Commission 
Sector Public
Country European Union (EU)
Start 02/2012 
End 01/2016
 
Description EU MULTISWARD
Amount £79,238 (GBP)
Funding ID 244983 
Organisation European Commission 
Sector Public
Country European Union (EU)
Start 03/2010 
End 02/2014
 
Description EU-SOLID
Amount £760,161 (GBP)
Funding ID 266367 
Organisation European Commission 
Sector Public
Country European Union (EU)
Start 03/2011 
End 02/2016
 
Description IBERS Studentship Chloroplastic phenolic oxidases
Amount £50,000 (GBP)
Organisation University of Wales 
Department Institute of Biological Environmental and Rural Sciences (IBERS)
Sector Academic/University
Country United Kingdom
Start 10/2010 
End 09/2013
 
Description ISPG Rumen Systems Biology
Amount £3,375,500 (GBP)
Funding ID BBS/E/W/10964A01 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 04/2012 
End 03/2017
 
Description International Workshop
Amount £10,630 (GBP)
Funding ID BB/1025212/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 09/2011 
End 10/2011
 
Description Postgraduate studentship Protein utilisation in forage fed animals
Amount £52,246 (GBP)
Organisation EBLEX 
Sector Charity/Non Profit
Country United Kingdom
Start 10/2009 
End 09/2012
 
Description Postgraduate studentship genetic basis for slow plant-mediated proteolysis in Festulolium hybrids
Amount £52,246 (GBP)
Organisation EBLEX 
Sector Charity/Non Profit
Country United Kingdom
Start 10/2010 
End 09/2013
 
Description Stapledon Fund Sumit Dagar
Amount £5,800 (GBP)
Organisation Stapledon Memorial Trust 
Sector Charity/Non Profit
Country United Kingdom
Start 04/2012 
End 09/2012
 
Description International partnership agreement, Bulgaria 
Organisation Bulgarian Academy of Sciences
Country Bulgaria 
Sector Learned Society 
PI Contribution Visit by Dr Kingston-Smith to the laboratory of Prof K Demirevska (Sofia, Bulgaria) to design the experimental plan in the subject area "The role of redox state on regulation of proteolysis under anoxia". Subsequent analysis of samples sent from Bulgaria to researchers in Aberystwyth.
Collaborator Contribution PI and Post-doctoral input to joint research project to establish and perform experiment, undertake analyses where possible.
Impact Simova-Stoilova LP, Demirevska KN, Kingston-Smith AH, Feller U. (2011) Involvement of the leaf antioxidant system in the response to soil flooding in two Trifolium genotypes differing in their tolerance to waterlogging. Plant Science.183: 43-49.
Start Year 2010
 
Description Prevotella microarray 
Organisation University of Illinois at Urbana-Champaign
Country United States 
Sector Academic/University 
PI Contribution Visit by Joan Edwards to laboratory of Prof Rod Mackie to carry out experimentation with Prevotella microarray available there.
Collaborator Contribution Access to facilities, intellectual input into experimental design and analysis.
Impact Conference presentations: Yeoman, C.J., Edwards, J.E., Cann, I.K., Morrison,M., Kingston-Smith, A.H. and Mackie, R.I. (2009) Transcriptional Analyses of Carbohydrate Metabolism in Prevotella ruminicola. Gordon Research Conference: Cellulosomes, Cellulases & Other Carbohydrate Modifying Enzymes Carl J. Yeoman, Joan Edwards, Alison Kingston-Smith and Roderick I. Mackie (2102) Transcriptional responses of Prevotella ruminicola reflect spatial and temporal differences in available nutrients from fresh perennial ryegrass. Rowett-INRA Gut Microbiology Symposium.
Start Year 2009
 
Description Dairy Science Forum 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Professional Practitioners
Results and Impact Talk by Alison Kingston-Smith on The role of plant enzymes in rumen proteolysis, presented new research information stimulating discussion about application and future plans.


Continued discussion on new forage varieties, promoted engagement of researchers and community.
Year(s) Of Engagement Activity 2008
 
Description Women in Food and Farming 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Presentation to WI group Women in Food and Farming as part of a workshop on GM crops. Audience included interested members of public, politicians, academics and activists.

Media coverage (local paper) of the event. Audience tended to be anti-GM so main impact was the ability to have a discussion of contrasting views.
Year(s) Of Engagement Activity 2011