Translation Grant for The HIPER project - Bringing the NMR Paradigm to ESR

Lead Research Organisation: University of St Andrews
Department Name: Physics and Astronomy

Abstract

In the original Basic Technology program Bringing the NMR Paradigm to ESR technology was developed to deliver complex sequences of very high power mm-wave pulses with sub-nanosecond resolution to a small sample in a strong magnetic field and measure a response from the sample 13 orders of magnitude lower in power, within 1 nanosecond of the final pulse. This was widely regarded as impossible by many leading members of the ESR instrumentation community three years ago. However, after two years developing the necessary mm-wave technologies and one year integrating them into a fully working system, the instrument is now demonstrating these very high levels of performance.The success of that program now opens up major new opportunities in the understanding of fast chemical and biochemical processes and in dramatically improving the sensitivity and resolution of techniques to understand local structure around paramagnetic centres, which often play a central role in determining the catalytic, enzymatic, electronic and optical properties of important chemical, biological and technological systems. It also promises to significantly improve the sensitivity of Site-Directed Spin Labelling techniques that promise to become a generic tool in the understanding of the structure, dynamics, conformational changes, interactions and function of important biomolecules in complex biomolecular processes. The aim of this Translation Grant is to provide underpinning but flexible support that will allow us to fully exploit this technological breakthrough via a series of pilot projects and collaborations.

Publications

10 25 50

publication icon
Dabb SL (2015) mer and fac isomerism in tris chelate diimine metal complexes. in Dalton transactions (Cambridge, England : 2003)

publication icon
Giannoulis A (2018) Orientation selection in high-field RIDME and PELDOR experiments involving low-spin Co ions. in Physical chemistry chemical physics : PCCP

publication icon
McKay J (2016) Compact Corrugated Feedhorns With High Gaussian Coupling Efficiency and $-60\;\text{dB}$ Sidelobes in IEEE Transactions on Antennas and Propagation

 
Description This project has developed new electron magnetic resonance instrumentation that increased the volume sensitivity by over an order of magnitude compared to standard commercial instruments, whilst improving time and spectral resolution. This instrument has won international awards and has resulted in many commercial sales. It is primarily used as a tool in molecular biology to understand large scale structure and interactions in proteins. Many of the technologies developed have been used within our radar programs. Multiple systems have been sold and the technologies developed used in many other research programs (radar, space, metrology)
Exploitation Route The new instrumentation, showed for the first time (against predictions in the literature) that it is possible to dramatically improve sensitivity in electron magnetic resonance experiments - which is the central grand challenge in this field. It achieved this by exploiting and developing new technologies and methodologies. We demonstrated gains in sensitivity by a factor of 30 and showed it was possible to measure signals during high power pulses.

The current EPR instrumentation supports the work of a large group of EPR research scientists from St Andrews and Dundee and their National and International collaborators.
Many of the technologies developed are used in related radar programs. Our commercial partner Thomas Keating Ltd have sold multiple commercial systems to international customers. We have just had a new EPSRC grant accepted to increase sensitivity by another factor of 30. At this level we should be able to detect spin labelled proteins at natural concentration in cells, detect defects at natural concentration levels in many common types of electronic thin film devices and look for trace radicals in large volume samples.
Sectors Chemicals,Electronics,Manufacturing, including Industrial Biotechology,Pharmaceuticals and Medical Biotechnology

URL http://www.st-andrews.ac.uk/~mmwave/
 
Description Over £2M in international commercial sales by our commercial partner Thomas Keating on mm-wave instrumentation, developed during the grant including a full system to the US National High Magnetic Field Laboratory in Florida and a partial system to PNNL in Washington State. Components developed under the Basic Technology program continue to be used by our commercial partner in a wide variety of instrumentation programs (metrology, space, radar, radiometry). Thomas Keating has sold EPR/DNP systems to approximately 10 major groups in Europe and 10 major groups in the US, and was awarded the Queens Award for Industry in 2012, with one third of turnover being directly attributed to magnetic resonance.
First Year Of Impact 2008
Sector Electronics,Manufacturing, including Industrial Biotechology
Impact Types Economic

 
Description Action Research Wellcome Trust
Amount £580,764 (GBP)
Funding ID Multi User Equipment Grant 099149/Z/12/Z 
Organisation Imperial College Healthcare NHS Trust 
Department Ovarian Cancer Action Research Centre (OCARC)
Sector Charity/Non Profit
Country United Kingdom
Start 09/2012 
End 08/2017
 
Description Action Research Wellcome Trust
Amount £1,400,000 (GBP)
Funding ID Wellcome Trust 
Organisation Imperial College Healthcare NHS Trust 
Department Ovarian Cancer Action Research Centre (OCARC)
Sector Charity/Non Profit
Country United Kingdom
Start  
 
Description Action Research Wellcome Trust
Amount £580,764 (GBP)
Funding ID Multi User Equipment Grant 099149/Z/12/Z 
Organisation Imperial College Healthcare NHS Trust 
Department Ovarian Cancer Action Research Centre (OCARC)
Sector Charity/Non Profit
Country United Kingdom
Start 09/2012 
End 08/2017
 
Description BBSRC Grouped
Amount £1,725,000 (GBP)
Funding ID BB/H017917/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start  
 
Description EPSRC
Amount £731,447 (GBP)
Funding ID EP/R013705/1 
Organisation Engineering and Physical Sciences Research Council (EPSRC) 
Sector Academic/University
Country United Kingdom
Start 12/2017 
End 11/2018
 
Description EPSRC
Amount £15,000 (GBP)
Funding ID EPSRC Strategic Partnership Fund 
Organisation Engineering and Physical Sciences Research Council (EPSRC) 
Sector Academic/University
Country United Kingdom
Start 11/2011 
End 06/2012
 
Description EPSRC
Amount £3,000,000 (GBP)
Funding ID CDT-lite scheme 
Organisation Engineering and Physical Sciences Research Council (EPSRC) 
Sector Academic/University
Country United Kingdom
Start 10/2008 
End 09/2017
 
Description EPSRC
Amount £52,536 (GBP)
Funding ID Sub-contract with Manchester 
Organisation Engineering and Physical Sciences Research Council (EPSRC) 
Sector Academic/University
Country United Kingdom
Start 07/2011 
End 06/2012
 
Description EPSRC
Amount £182,257 (GBP)
Funding ID Outreach Grant 
Organisation Engineering and Physical Sciences Research Council (EPSRC) 
Sector Academic/University
Country United Kingdom
Start 03/2010 
End 03/2013
 
Description EPSRC IAA
Amount £22,386 (GBP)
Organisation Engineering and Physical Sciences Research Council (EPSRC) 
Sector Academic/University
Country United Kingdom
Start 01/2016 
End 03/2017
 
Description EPSRC IAA
Amount £17,567 (GBP)
Funding ID EP/R511778/1 
Organisation Engineering and Physical Sciences Research Council (EPSRC) 
Sector Academic/University
Country United Kingdom
Start 10/2017 
End 09/2018
 
Description EPSRC IAA
Amount £23,071 (GBP)
Funding ID EP/R511778/1 
Organisation Engineering and Physical Sciences Research Council (EPSRC) 
Sector Academic/University
Country United Kingdom
Start 04/2018 
End 03/2019
 
Description European Commission (EC)
Amount £836,853 (GBP)
Funding ID 312745 
Organisation European Commission 
Sector Public
Country European Union (EU)
Start  
 
Description Home Office
Amount £49,602 (GBP)
Funding ID HOS/12/024 
Organisation Home Office 
Sector Public
Country United Kingdom
Start 02/2013 
End 09/2013
 
Description Medical Research Council
Amount £438,880 (GBP)
Funding ID MRC Project Grant 
Organisation Medical Research Council (MRC) 
Sector Academic/University
Country United Kingdom
Start 01/2011 
End 12/2013
 
Description Thales Ltd
Amount £21,991 (GBP)
Funding ID P O 7038156 
Organisation Thales Group 
Sector Private
Country France
Start 01/2013 
End 09/2013
 
Description Thomas Keating Ltd
Amount £40,000 (GBP)
Funding ID Royalties 
Organisation Thomas Keating Ltd 
Sector Private
Country United Kingdom
Start  
 
Description Thomas Keating Ltd
Amount £40,000 (GBP)
Funding ID Royalties 
Organisation Thomas Keating Ltd 
Sector Private
Country United Kingdom
Start  
 
Description US Army Research Laboratory
Amount £62,515 (GBP)
Funding ID W911NF-12-1-0535 
Organisation US Army Research Lab 
Sector Public
Country United States
Start 09/2012 
End 03/2013
 
Description US National High Magnetic Field Lab
Amount £1,100,000 (GBP)
Funding ID HIPER 
Organisation US National High Magnetic Field Laboratory 
Sector Public
Country United States
Start 12/2011 
End 01/2013
 
Description US National High Magnetic Field Lab
Amount £1,100,000 (GBP)
Funding ID HIPER 
Organisation US National High Magnetic Field Laboratory 
Sector Public
Country United States
Start 09/2011 
End 09/2013
 
Description US National High Magnetic Field laboratory
Amount £99,990 (GBP)
Organisation Florida State University 
Sector Academic/University
Country United States
Start 01/2015 
End 04/2016
 
Title Orientational PELDOR 
Description The method uses the resolution and sensitivity available at high magnetic fields to determine the relative orientation (and associated distribution) of spin labels attached to biological samples 
Type Of Material Biological samples 
Year Produced 2009 
Provided To Others? Yes  
Impact It is now an established method - although the impact is likely to be in the next few years with the development of more rigid spin labels. 
 
Description Thomas Keating Ltd 
Organisation Thomas Keating Ltd
Country United Kingdom 
Sector Private 
PI Contribution We were joint partners under the original Basic Technology Program. The overall system design and design of many critical components were made by the group at St Andrews.
Collaborator Contribution Thomas Keating supplied expertise in manufacturing and in some component design and provided a commercial route to exploit the technologies.
Impact Over £2M in instrumentation and component sales related to technologies and instrumentation developed under the grant. In 2012 Thomas Keating Ltd won the Queens Award for Industry - for exports - one third was attributed to suppling magnetic resonance instrumentation. Sales to groups in Europe include: CNRS Grenoble, EPFL Lausanne, Heidelberg, Pisa, Saclay, Nottingham, Warwick, Weizmann Institute, Mulheim, Berlin, Stuttgart, Frankfurt Sales in the US include: MIT, NHMFL, Cornell, Yale, UF Gainesville, North Eastern, NorthWestern, John Hopkins, PNNL, UCSB.
Start Year 2008