Sculpting Dynamic Amphiphilic Structures
Lead Research Organisation:
Imperial College London
Department Name: Chemistry
Abstract
Biomembranes lie at the heart of most biological function, and lipid membranes are increasingly finding a wide range of novel applications in biotechnology and nanomedicine. Such self-assembled amphiphilic interfaces can adopt an astonishing range of complex shapes and liquid-crystalline structures ordered in 1, 2 or 3 dimensions, over length scales stretching from 2 - 3 nanometres, to microns. Gaining an understanding at a molecular level of how interface structure, ordering, dynamics and micromechanics depend upon chemical structure and composition, and thermodynamic variables such as temperature, hydration, and pressure, is the key to learning how we can manipulate such self-assembled soft interfaces to create novel and useful structures and new technologies, and this is the main aim of this Programme.
We have identified three key underpinning basic science challenges: 1) asymmetry; 2) patterning; 3) curvature, long-range organisation and symmetry. There are four main aspects underlying these challenges which we consider are of crucial importance: i) compositional asymmetry and dynamics of amphiphile flip-flop across bilayers; ii) lateral segregation, line tension and microdomain formation; iii) membrane curvature and curvature elasticity; iv) charge and dipolar interactions between lipid headgroups. Furthermore, there is a complicated coupling between all of these four aspects, and this is where we will focus much of our attention.
We have assembled a team of five leading UK University research groups, spanning Chemistry, Physics and Biophysics. The groups have complementary expertise covering laboratory-based and synchrotron time-resolved X-ray diffraction, neutron scattering, solid-state nuclear magnetic resonance, calorimetry, biomolecular force microscopy, Langmuir trough and microfluidics technologies, linear and non-linear spectroscopies, atomic force microscopy, spectroscopic and optical imaging, optical tweezers, microrheology, and theory. These approaches will be used to attack different inter-related aspects of the three key basic science challenges. We will ensure an efficient translation and synthesis of all of the findings, by a tightly- regulated management structure, and by regular meetings and staff exchanges between the five research groups.
Building on the engineering rules and technologies developed previously in the programme, we will integrate the earlier work to develop lipid structures into active lipid systems such as: self-encapsulated droplet interface bilayer networks in water; patterned asymmetric vesicles of defined size: coupling microfluidics with smart droplet microtools; phospholipid phases and vesicles in thermal gradients.
We will then use this knowledge to develop three demonstration systems:
i) Artificial Organelles. The development of artificial organelle machines which mimic some of the remarkable functions and properties of biology will lead to new approaches for personalized healthcare.
ii) Rapid drug-membrane binding screen. A compartmentalised, rapid drug screening device will allow parallel measurements of drug interactions with a number of artificial plasma membrane mimics (PMMs) formed by an array of parallel droplet interface bilayer or vesicle networks.
iii) In-Cubo Crystallization of Large Membrane Proteins. Learning how to swell lipid cubic phases will unlock our ability to construct cubic scaffolds with unit cell dimensions of the order of tens or hundreds of nanometres, allowing incorporation of large membrane proteins (>50kD), which are major drug targets for the pharmaceutical industry.
Further biological and biotechnological applications will be developed during the course of the Programme by the current Investigators and a wider group of industrial and academic collaborators, who will be brought into the Programme as appropriate.
We have identified three key underpinning basic science challenges: 1) asymmetry; 2) patterning; 3) curvature, long-range organisation and symmetry. There are four main aspects underlying these challenges which we consider are of crucial importance: i) compositional asymmetry and dynamics of amphiphile flip-flop across bilayers; ii) lateral segregation, line tension and microdomain formation; iii) membrane curvature and curvature elasticity; iv) charge and dipolar interactions between lipid headgroups. Furthermore, there is a complicated coupling between all of these four aspects, and this is where we will focus much of our attention.
We have assembled a team of five leading UK University research groups, spanning Chemistry, Physics and Biophysics. The groups have complementary expertise covering laboratory-based and synchrotron time-resolved X-ray diffraction, neutron scattering, solid-state nuclear magnetic resonance, calorimetry, biomolecular force microscopy, Langmuir trough and microfluidics technologies, linear and non-linear spectroscopies, atomic force microscopy, spectroscopic and optical imaging, optical tweezers, microrheology, and theory. These approaches will be used to attack different inter-related aspects of the three key basic science challenges. We will ensure an efficient translation and synthesis of all of the findings, by a tightly- regulated management structure, and by regular meetings and staff exchanges between the five research groups.
Building on the engineering rules and technologies developed previously in the programme, we will integrate the earlier work to develop lipid structures into active lipid systems such as: self-encapsulated droplet interface bilayer networks in water; patterned asymmetric vesicles of defined size: coupling microfluidics with smart droplet microtools; phospholipid phases and vesicles in thermal gradients.
We will then use this knowledge to develop three demonstration systems:
i) Artificial Organelles. The development of artificial organelle machines which mimic some of the remarkable functions and properties of biology will lead to new approaches for personalized healthcare.
ii) Rapid drug-membrane binding screen. A compartmentalised, rapid drug screening device will allow parallel measurements of drug interactions with a number of artificial plasma membrane mimics (PMMs) formed by an array of parallel droplet interface bilayer or vesicle networks.
iii) In-Cubo Crystallization of Large Membrane Proteins. Learning how to swell lipid cubic phases will unlock our ability to construct cubic scaffolds with unit cell dimensions of the order of tens or hundreds of nanometres, allowing incorporation of large membrane proteins (>50kD), which are major drug targets for the pharmaceutical industry.
Further biological and biotechnological applications will be developed during the course of the Programme by the current Investigators and a wider group of industrial and academic collaborators, who will be brought into the Programme as appropriate.
Planned Impact
Our ambition within this Programme grant is to exploit the emerging field of molecular membrane engineering, by designing and constructing new biologically-inspired parts, devices and systems with user-defined levels of asymmetry, patterning and curvature. This will revolutionise the design and fabrication of smart, soft materials based on the lipid bilayer as the basic structural motif, with morphologies spanning the nano to micron lengthscale, and dynamics controlled over a wide range of timescales. In the longer term this will lead to a paradigm shift in areas such as nanomedicine, bioelectronics, biological computing devices and synthetic cells.
To achieve this we will bring together five leading UK research groups to investigate experimentally and theoretically the rules underpinning the self-assembly, ordering and dynamics of amphiphilic systems.
This knowledge will then be exploited:
i) to develop quantitative engineering rules for construction of 2-D and 3-D membrane-based structures with targeted asymmetry, patterning and curvature, focussing initially on purely lipid-based systems;
ii) to design self-assembled, fluid, amphiphilic systems with useful functional properties;
iii) to develop demonstrate applications, such as artificial organelles, high-throughput screens for drug discovery, and swollen cubic phases for membrane protein crystallisation.
We plan to invite companies with interests in these areas to participate by funding Industrial PhD Students from year 3 onwards to develop the applications, and to help embed the knowledge gained within an industrial context. We will spend £10k from our own resources on direct commercialisation activities, led by the ICB and the PM. They, together with the consortium, will:
a) network with relevant TSB Knowledge Transfer Networks and other industrial networks that may have an interest in these technologies;
b) develop Outline Concept Specifications documents as a starting point for discussions with potential users and developers of the technologies;
c) form an Industry Consortium. In the final year of the project, an Industrial Showcase event will be held at Durham.
As this project extends the current state-of-the art we expect to generate intellectual property with significant commercial value, and will protect it via established technology transfer organisations who will cover the patenting costs. A consortium agreement will govern the IP generated, and the Advisory Board will be bound by a confidentiality agreement.
We will organise a Workshop in year 3, with invitees from both academia and industry, and an International Meeting in year 5. We will embed public engagement activities within the Programme, such as the Royal Society's Summer Science Exhibition. We will highlight the field of self-assembled complex soft materials by making conference lectures freely available on iTunesU /YouTube and by a radio broadcast produced as part of the Imperial College Institute of Chemical Biology DTC Science Communication course. This will complement dissemination via websites, meetings, conferences and journal publications.
We expect the more junior Investigators and some of the post-doctoral researchers to become research leaders, either in industry or academia, within 5-10 years.
To achieve this we will bring together five leading UK research groups to investigate experimentally and theoretically the rules underpinning the self-assembly, ordering and dynamics of amphiphilic systems.
This knowledge will then be exploited:
i) to develop quantitative engineering rules for construction of 2-D and 3-D membrane-based structures with targeted asymmetry, patterning and curvature, focussing initially on purely lipid-based systems;
ii) to design self-assembled, fluid, amphiphilic systems with useful functional properties;
iii) to develop demonstrate applications, such as artificial organelles, high-throughput screens for drug discovery, and swollen cubic phases for membrane protein crystallisation.
We plan to invite companies with interests in these areas to participate by funding Industrial PhD Students from year 3 onwards to develop the applications, and to help embed the knowledge gained within an industrial context. We will spend £10k from our own resources on direct commercialisation activities, led by the ICB and the PM. They, together with the consortium, will:
a) network with relevant TSB Knowledge Transfer Networks and other industrial networks that may have an interest in these technologies;
b) develop Outline Concept Specifications documents as a starting point for discussions with potential users and developers of the technologies;
c) form an Industry Consortium. In the final year of the project, an Industrial Showcase event will be held at Durham.
As this project extends the current state-of-the art we expect to generate intellectual property with significant commercial value, and will protect it via established technology transfer organisations who will cover the patenting costs. A consortium agreement will govern the IP generated, and the Advisory Board will be bound by a confidentiality agreement.
We will organise a Workshop in year 3, with invitees from both academia and industry, and an International Meeting in year 5. We will embed public engagement activities within the Programme, such as the Royal Society's Summer Science Exhibition. We will highlight the field of self-assembled complex soft materials by making conference lectures freely available on iTunesU /YouTube and by a radio broadcast produced as part of the Imperial College Institute of Chemical Biology DTC Science Communication course. This will complement dissemination via websites, meetings, conferences and journal publications.
We expect the more junior Investigators and some of the post-doctoral researchers to become research leaders, either in industry or academia, within 5-10 years.
Publications
Meredith SA
(2021)
Model Lipid Membranes Assembled from Natural Plant Thylakoids into 2D Microarray Patterns as a Platform to Assess the Organization and Photophysics of Light-Harvesting Proteins.
in Small (Weinheim an der Bergstrasse, Germany)
Mezzenga R
(2019)
Nature-Inspired Design and Application of Lipidic Lyotropic Liquid Crystals.
in Advanced materials (Deerfield Beach, Fla.)
Miller D
(2013)
Protocell design through modular compartmentalization
in Journal of The Royal Society Interface
Miller DM
(2016)
Light-activated control of protein channel assembly mediated by membrane mechanics.
in Nanotechnology
Mognetti BM
(2019)
Programmable interactions with biomimetic DNA linkers at fluid membranes and interfaces.
in Reports on progress in physics. Physical Society (Great Britain)
Paillusson F
(2016)
Phase Separation on Bicontinuous Cubic Membranes: Symmetry Breaking, Reentrant, and Domain Faceting
in Physical Review Letters
Parolini L
(2016)
Controlling Self-Assembly Kinetics of DNA-Functionalized Liposomes Using Toehold Exchange Mechanism.
in ACS nano
Parolini L
(2015)
Volume and porosity thermal regulation in lipid mesophases by coupling mobile ligands to soft membranes.
in Nature communications
Description | We have carried out measurements - some for the first time - of: a. Effect of membrane asymmetry on bilayer rigidity; b. Effect of hydrostatic pressure on the mechanical properties of lipid bilayers; c. Lifetime of membrane asymmetry using fluctuation analysis/SFG; d. Interleaflet registration in phase-separated bilayers; e. Coupling mobile ligands to soft membranes; f. Soft pinning of liquid domains on caps; g. Diffusion in lipid membranes; h. Critical fluctuations in lipid mixtures by AFM. We have developed: a. Highly swollen cubic phases with dimensions comparable with those seen in Nature; b. Vesicles with user defined size, asymmetry, cargo and biomolecule content; c. Compartmentalised vesicles; d. Extended 2-D/3-D droplet interface bilayer networks; e. Compartmentalised extended lifetime multisomes; f. Membrane encapsulated nanoparticles. In terms of applications, we have developed: a. Artificial cells capable of acting as micro-reactors, as well as performing in-situ drug synthesis, environmental sensing and smart delivery. The complexity of these systems is now such that we have transplanted spatially separated enzymatic pathways into artificial protocells; b. High-throughput screens for drug discovery; c. Studies of membrane translocation profiles for agrichemicals (Syngenta), PROTAC molecules (GSK plc) and hair additives (P&G) using droplet interface bilayer networks. |
Exploitation Route | By bringing together five world leading UK research groups, this Programme grant has pioneered a series of breakthroughs in the emerging field of molecular membrane engineering that have already supported 40 publications, 38 high profile international conferences, 33 oral and poster presentations, £20M+ in follow-on funding and 3 industrial collaborations. The successful development of quantitative engineering rules for the construction of 2-D and 3-D membrane-based structures with user defined levels of asymmetry, patterning and curvature has enabled us to extend the aims and ambitions of the project beyond proof of concept applications to include direct commercial exploitation. New collaborations with leading technology companies in the pharmaceutical (GSK plc), agri-tech (Syngenta) and personal care sectors (P&G) should led to the translation of the innovations from the Programme grant into the industrial sector. |
Sectors | Agriculture Food and Drink Chemicals Healthcare Manufacturing including Industrial Biotechology Pharmaceuticals and Medical Biotechnology |
Description | This Programme grant has had a huge impact in the field. It has led to over 150 refereed publications. The Investigators have given numerous Invited Lectures at international conferences as well as many Seminars and outreach talks to a more general audience. They have also organised key conferences in the field, such as the Telluride Workshop on Physics and Chemistry of Lipid Membranes, 4 - 8 July 2016, USA; an RSC Faraday Discussion on Peptide - Membrane Interactions, 8 - 10 Sept 2021 (held online); a Royal Society Discussion Meeting on Cell Mimicry, 7 - 8 Nov 2022, Royal Society, London. The Imperial team have been recognized by the Imperial College President's Award for Outstanding Research Team in 2015, and the Rita and John Cornforth Award of the RSC in 2017. This latter award was linked to a series of Award Lectures, given by the team at the Universities of Brighton, Lincoln, Southampton, Queens University Belfast, and UCL. Members of the team have sat on a range of Review Panels (EPSRC, ESRF, Diamond). There are numerous examples of Outreach, for example schools lectures, and most recently the Imperial team will have a stall on 'Artificial Cells' at the Great Exhibition Road Festival 2023, from 17-18 June 2023. |
First Year Of Impact | 2015 |
Sector | Agriculture, Food and Drink,Chemicals,Education,Healthcare,Manufacturing, including Industrial Biotechology,Pharmaceuticals and Medical Biotechnology |
Impact Types | Cultural Societal Policy & public services |
Description | Biophysical Sciences Institute Summer undergraduate research studentship |
Amount | £2,000 (GBP) |
Organisation | Durham University |
Department | Biophysical Sciences Institute |
Sector | Academic/University |
Country | United Kingdom |
Start | 06/2014 |
End | 09/2014 |
Description | Leverhulme Doctoral Scholarship Programme |
Amount | £1,000,000 (GBP) |
Organisation | The Leverhulme Trust |
Sector | Charity/Non Profit |
Country | United Kingdom |
Start | 09/2018 |
End | 09/2021 |
Title | 0 |
Description | |
Type Of Material | Database/Collection of data |
Year Produced | 2019 |
Provided To Others? | Yes |
Title | Dataset associated with "Nanoscale Substrate Roughness Hinders Domain Formation in Supported Lipid Bilayers" |
Description | Supported Lipid Bilayers (SLBs) are model membranes formed at solid substrate surfaces. This architecture renders the membrane experimentally accessible to surface sensitive techniques used to study their properties, including Atomic Force Microscopy (AFM), optical fluorescence microscopy, Quartz Crystal Microbalance (QCM) and X-Ray/Neutron Reflectometry, and allows integration with technology for potential biotechnological applications such as drug screening devices. The experimental technique often dictates substrate choice or treatment, and it is anecdotally recognised that certain substrates are suitable for the particular experiment, but the exact influence of the substrate has not been comprehensively investigated. Here, we study the behavior of a simple model bilayer, phase separating on a variety of commonly used substrates, including glass, mica, silicon and quartz, with drastically different results. The distinct micron scale domains observed on mica, identical to those seen in free-floating Giant Unilamellar Vesicles (GUVs), are reduced to nanometer scale domains on glass and quartz. The mechanism for the arrest of domain formation is investigated, and the most likely candidate is nanoscale surface roughness, acting as a drag on the hydrodynamic motion of small domains during phase separation. Evidence was found that the physico-chemical properties of the surface have a mediating effect, most likely due to changes in the lubricating interstitial water layer between surface and bilayer. |
Type Of Material | Database/Collection of data |
Year Produced | 2020 |
Provided To Others? | Yes |
URL | http://archive.researchdata.leeds.ac.uk/796/ |
Title | Research Data Supporting "A Robust Liposomal Platform For Direct Colorimetric Detection Of Sphingomyelinase Enzyme And Inhibitors" |
Description | Raw research data supporting the publication: Holme, M. N. et al., ACS Nano, 2018, DOI: 10.1021/acsnano.8b03308. |
Type Of Material | Database/Collection of data |
Year Produced | 2018 |
Provided To Others? | Yes |
URL | https://zenodo.org/record/1293379 |
Title | Research Data Supporting "A Robust Liposomal Platform For Direct Colorimetric Detection Of Sphingomyelinase Enzyme And Inhibitors" |
Description | Raw research data supporting the publication: Holme, M. N. et al., ACS Nano, 2018, DOI: 10.1021/acsnano.8b03308. |
Type Of Material | Database/Collection of data |
Year Produced | 2018 |
Provided To Others? | Yes |
Title | Research Data in support of: Crystallization of Amphiphilic DNA C-Stars |
Description | Confocal microscopy z-stacks of aggregates of Alexa-488 labelled C-stars with n=3,4,6. |
Type Of Material | Database/Collection of data |
Year Produced | 2017 |
Provided To Others? | Yes |
URL | https://www.repository.cam.ac.uk/handle/1810/266290 |
Title | Research Data: Membrane Adhesion through Bridging by Multimeric Ligands |
Description | File formatt is .tiff, with each .tiff file representing a single frame of video or slice of stack. Individual vesicles are organised into .zip files corresponding to their SA/DNA concentration ratios and experiment type: Z-Stack, Flickering or FRAP. In the Z-stacks .zip files, two channels were recorded, using a 488nm and 633nm laser respectively. For each vesicle folder, there is a separate subfolder for each of these channels channel. Each frame of the Z-stacks represents a 0.1um slice. In Flickering videos, each .tiff file is a frame of a 1000 frame video, with a framerate of 97ms/frame, taken at the equator of the vesicle. In FRAP experiments, each vesicle subfolder contains subfolders at different frame rates. These are: StagePre (10fps), StagePost1 (10fps), StagePost2 (0.5fps) and StagePost3 (0.1fps). These correspond to stages of the pre- and post-bleaching process. |
Type Of Material | Database/Collection of data |
Year Produced | 2017 |
Provided To Others? | Yes |
URL | https://www.repository.cam.ac.uk/handle/1810/266729 |
Title | Research data supporting "Amphiphilic-DNA Platform for the Design of Crystalline Frameworks with Programmable Structure and Functionality" |
Description | Figure4_X: Confocal microscopy z-stacks and images of C-star aggregates soaked in fluorescent molecule X, used for the penetration assay presented in figure 4 of the associated publication. Figure 5: Confocal microscopy z-stacks and images of C-star aggregates used for the NTA-GFP binding assay presented in figure 5a-b of the associated publication. XrayData: 2D SAXS patterns of C-star aggregates, associated buffers, and silver behenate calibrant collected at the Diamond Light Source beamline I11. |
Type Of Material | Database/Collection of data |
Year Produced | 2019 |
Provided To Others? | Yes |
URL | https://www.repository.cam.ac.uk/handle/1810/288082 |
Title | Research data supporting "Controlling Self-Assembly Kinetics of DNA-Functionalized Liposomes Using Toehold Exchange Mechanism" |
Description | The dataset contains data on aggregation and melting experiments in samples of DNA-functionalised liposomes as described in the paper "Controlling Self-Assembly Kinetics of DNA-Functionalized Liposomes Using Toehold Exchange Mechanism." (ACS Nano doi:10.1021/acsnano.5b07201). Each compressed folder labelled as "AGG_TQxxC_Ryyy.zip" contains raw data on aggregation experiments of samples with stoichiometric ratio R=yyy (yyy=0.00, 0.25, 0.50, 0.75, 1.00) incubated at TQ=xx (xx=35, 30, 25, 20, 15) degrees Celsius. Please refer to the paper for details. The data are full resolution epifluorescence microscopy images in .tiff format. Individual images are labelled as "Black_N", "ImZ1_N", "ImZ2_N", and "ImZ3_N". The integer index N=1,...,NMax indicates time, with NMax being different from sample to sample. The physical time corresponding to each time-step is reported in the text file "time.dat" as a string of comma separated numbers. Each compressed "AGG_TQxxC_Ryyy.zip" folder contains the corresponding "time.dat" file. Images labelled as "Black_N" are captured with the fluorescence illumination disabled, ad used for subtracting background signal. Images labelled as "ImZ1_N", "ImZ2_N", and "ImZ3_N" contain snapshots of the samples taken at 3 different vertical positions spaced by 1.00 microns from each other. The data on the manuscript are obtained using the average between these three images diminished by the corresponding "Black_N". Compressed folders labelled "MELT_TQxxC_Ryyy.zip" contain data on the melting experiments on the same samples of the corresponding aggregation experiments. Image files inside are named according to the same scheme, but here the index "N" corresponds different temperatures, increasing stepwise from TQ. The sample temperature measured for each "N" is reported in the corresponding text file "TemperatureRamp_TQxxC.dat" in comma-separated format. Note that the temperature files TemperatureRamp_TQxxC.dat are not included in the compressed folders "MELT_TQxxC_Ryyy.zip" and need to be downloaded separately. The data shown in Figure 2 of the paper are obtained by averaging over three similar datasets. Analysis methods are described in the Methods section of the paper. |
Type Of Material | Database/Collection of data |
Year Produced | 2016 |
Provided To Others? | Yes |
URL | https://www.repository.cam.ac.uk/handle/1810/253615 |
Title | Research data supporting "Flexibility defines structure in crystals of amphiphilic DNA nanostars" |
Description | Zip folder containing raw 2D SAXS patterns of aggregates of each of the cholesterol functionalised DNA nanostar designs presented in the main text, in both buffer conditions tested. All SAXS data were collected at the Diamond Light Source, beamline I22 during two visits (visit ID's sm16024 and sm17271). A folder for each visit can be found within the Zipped folder. Within each of these folders can be found the 2D SAXS patterns of the buffer, samples investigated (naming convention as in main text), and silver behenate (AgBe) calibrant. See 'readme' for further information. |
Type Of Material | Database/Collection of data |
Year Produced | 2019 |
Provided To Others? | Yes |
URL | https://www.repository.cam.ac.uk/handle/1810/288790 |
Title | Research data supporting "Kinetics of Nanoparticle-Membrane Adhesion Mediated by Multivalent Interactions" |
Description | The dataset contains experimental and simulation data on the interaction of gold nanoparticles (NPs) and DNA-functionalised liposomes as described in the paper "Kinetics of Nanoparticle-Membrane Adhesion Mediated by Multivalent Interactions". Experimental raw data are divided into two compressed folders relative to DLS and UV-vis experiments, and named accordingly. Simulation data are in a separate compressed folder (SimulationData). The "DLS" folder contains sub-folders labelled "LUVXXX", where XXX indicates the number of receptors tethered on each liposome in the system, accordingly to Table 1 in the main text of the paper (370, 770, 1190, 1640). Each folder contains tab-separated text files labelled either as "Data_ratio*.txt" or "Time_ratio*.txt", where "*" indicates the ratio between the number of NPs and LUVs in the system, as reported in Table 1 of the main text (7, 70, 290). "Data_ratio*.txt" is a multi-column tab-separated file containing the raw DLS autocorrelation functions. The first column contains the delay times ("tau", see associated publication), while in the other columns (from the second to the last) contain the autocorrelation values acquired at different times "t" (see associated publication) from the beginning of the experiment. The file "Time_ratio*.txt" is a single column file containing the list of times "t" associated to each autocorrelation function of the corresponding "Data_ratio*.txt". These data have been used to produce figures 3, 4a, 4b, 6a, 6b and 7 as described in the associated publication. The folder named "Controls" contains, in the same format, data associated to control experiments used to produce figure S5.1 in the Supporting Information. The "UV-vis" folder contains raw data underlying figure S3.1 of the Supporting Information. Each multi-column tab-separated text file is labelled as "Melting_sampleY", where Y=1, 2, 3 correspond to the three data points in figure S3.1, with increasing rho_R-LUV (horizontal axis in the figure). Each file contains two or three sets of data in a four-column format. These different sets correspond to repetitions of the same experiment. In each 4-column set, the first and the third column contain temperature (in degrees Celsius), column 2 contains the absorbance data measured at the plasmon-resonance frequency of free NPs at the temperatures reported in column 1, while column 4 contains the absorbance data recorded at the plasmon-resonance frequency of NP-LUV complexes at the temperatures reported in column 3. The "SimulationData" folder contains 4 other folders, each corresponding to the different figures and panels featuring simulation data: FIGURE_4C, FIGURE_5A, FIGURE_5B and FIGURE_6A. All folders contain multiple .txt files, each corresponding to the different curves in the figures. All files contain two space-separated columns corresponding to the quantities on the horizontal and vertical axes of the figures. FIGURE_4C contains simulated data for the NP-LUV adhesion time as a function of the receptor surface density for three different values of the NP-LUV concentration ratio (7, 70, 290). FIGURE_5A contains simulated data for the number of NP adhering on the LUV as a function of time for 5 values of the simulation on-rate (1E-3, 1E-2, 1E-1, 1E5, infinity). Time is expressed in seconds. FIGURE_5B contains the average number of ligand-receptor bonds per NP VS time for the same values of the on-rate of FIGURE_5A. FIGURE_6A contains the simulated steady-state fraction of adhering NPs as a function of the receptor surface density for three different values of the NP-LUV concentration ratio (7, 70, 290). |
Type Of Material | Database/Collection of data |
Year Produced | 2019 |
Provided To Others? | Yes |
URL | https://www.repository.cam.ac.uk/handle/1810/291337 |
Title | Research data supporting: Thermal driven domain and cargo transport in lipid membranes |
Description | The dataset contains data on the motion and distribution of lipid domains on the membrane of giant unilamellar vesicles in thermal gradients. Each compressed folder contains .tiff images of the fluorescence microscopy data for phase separated vesicles, displayed as Matlab plots with axes labelled in pixels. Each pixel is equal to 0.145 micrometers. There is one compressed folder including the data for each figures 1C, 2B, 2C, 2D, and 6B. As well as images used to track the domains for Fig. 6A. Please refer to the paper for further details. Images are given for the hot and cold caps of vesicles at time t =xmin i.e. folder "t 0min cold" contains images of the cold side of vesicles in a thermal gradient after 0 minutes. The data in the manuscript are obtained from an average of e.g. the domain spacing, domain number or area fraction of white domains for each vesicle in the corresponding folder. |
Type Of Material | Database/Collection of data |
Year Produced | 2017 |
Provided To Others? | Yes |
URL | https://www.repository.cam.ac.uk/handle/1810/261806 |
Description | Jonas Camillus Jeppesen - visiting international PhD student from the lab of Adam Simon Cohenson in Odense, Denmark |
Organisation | University of Southern Denmark |
Country | Denmark |
Sector | Academic/University |
PI Contribution | Access to high-speed AFM, training, and techniques to study membrane dynamics with temperature control. Collaborator brought new samples and system well studied in their lab. The project was to study the early stages of domain nucleation in gel-phase binary lipid mixtures. |
Collaborator Contribution | None |
Impact | None-to-date, work still in progress |
Start Year | 2015 |
Description | CECAM Workshop |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Postgraduate students |
Results and Impact | Workshop entitled "Multi scale approaches to nano-structured soft matter: from amphiphiles to block copolymers". |
Year(s) Of Engagement Activity | 2018 |
Description | Co-organiser, Royal Society Discussion Meeting on 'Cell mimicry: bottom-up engineering of life' |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Policymakers/politicians |
Results and Impact | Co-organiser, Royal Society Discussion Meeting on 'Cell mimicry: bottom-up engineering of life', 21st - 22nd September 2020, Royal Society London. |
Year(s) Of Engagement Activity | 2020 |
Description | Crossing BioMembranes NET Conference, September 2015 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Other audiences |
Results and Impact | CBM (Crossing BioMembranes) NET Conference, September 2015. |
Year(s) Of Engagement Activity | 2015 |
Description | Deputy Chair of Scientific Committee |
Form Of Engagement Activity | A formal working group, expert panel or dialogue |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Study participants or study members |
Results and Impact | Deputy Chair of Scientific Committee: Faraday Discussion on Peptide - Membrane Interactions, Royal Society of Chemistry, London, 8th - 10th September 2021. Held Online. |
Year(s) Of Engagement Activity | 2021 |
Description | Deputy Chair of Scientific Committee, Faraday Discussion |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Policymakers/politicians |
Results and Impact | Deputy Chair of Scientific Committee, Faraday Discussion on Peptide - Membrane Interactions, Royal Society of Chemistry, London, 7th - 9th September 2020. |
Year(s) Of Engagement Activity | 2020 |
Description | Drug Disposition Symposium, Stevenage, June 2015 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Public/other audiences |
Results and Impact | Drug Disposition Symposium, Stevenage, June 2015 |
Year(s) Of Engagement Activity | 2015 |
Description | Faraday Discussion on Peptide - Membrane Interactions |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Supporters |
Results and Impact | Faraday Discussion on Peptide - Membrane Interactions, RSC, London, 8 - 10 Sept 2021 |
Year(s) Of Engagement Activity | 2021 |
Description | Graduate school teaching, ICTP Trieste, July 2014 |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Postgraduate students |
Results and Impact | Pietro Cicuta was involved in a hands-on graduate school in Trieste, Italy. This event increased discussion and knowledge transfer Close interactions with graduate students and young faculty from developing countries helped to introduce new tools and techniques to the group, encouraging the development of scientific leaders in less developed countries |
Year(s) Of Engagement Activity | 2014 |
Description | Halim Kusumaatmaja Invited Conference talk - 115th Statistical Mechanics Conference, Rutgers University, USA |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other audiences |
Results and Impact | Surveying Free Energy Landscapes: Applications to Continuum Soft Matter Systems, Rutgers University, USA |
Year(s) Of Engagement Activity | 2016 |
Description | Halim Kusumaatmaja Invited Conference talk - : Fundamentals and Applications of Lipid Membrane Biophysics, London, UK |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Other audiences |
Results and Impact | Continuum Theory of Lipid Membranes, London, UK |
Year(s) Of Engagement Activity | 2015 |
Description | Halim Kusumaatmaja Invited Conference talk - Partial Order: Mathematics, Simulations and Applications, Institute for Pure and Applied Mathematics, UCLA, Los Angeles, USA |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other audiences |
Results and Impact | Surveying Free Energy Landscapes: Applications to Continuum Soft Matter Systems, UCLA, USA |
Year(s) Of Engagement Activity | 2016 |
Description | Halim Kusumaatmaja Seminar talk - Department of Mathematics, Pennsylvania State University, USA |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other audiences |
Results and Impact | Wetting on Lipid Membranes, Pennsylvania, USA |
Year(s) Of Engagement Activity | 2016 |
Description | Halim Kusumaatmaja Seminar talk - Department of Mathematics, University of York, UK |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Other audiences |
Results and Impact | Membrane Phase Separation, York, UK |
Year(s) Of Engagement Activity | 2015 |
Description | Halim Kusumaatmaja Seminar talk - Department of Physics, University of Oxford, UK |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Other audiences |
Results and Impact | Phase Separation on Bicontinuous Cubic Membranes, Oxford, UK |
Year(s) Of Engagement Activity | 2016 |
Description | Halim Kusumaatmaja Seminar talk - Institut Lumière Matière |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Other audiences |
Results and Impact | Phase Separation on Bicontinuous Cubic Membranes, Lyon, France |
Year(s) Of Engagement Activity | 2017 |
Description | Halim Kusumaatmaja Seminar talk - State Key Laboratory of Polymer Physics and Chemistry, Changchun, China |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other audiences |
Results and Impact | Curvature-Induced Phase Separation on Lipid Membranes, Changchun, China |
Year(s) Of Engagement Activity | 2015 |
Description | Halim Kusumaatmaja Seminar talk - University of Rome Tor Vergata, Rome, Italy |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other audiences |
Results and Impact | Phase Separation on Bicontinuous Cubic Membranes, Rome, Italy |
Year(s) Of Engagement Activity | 2015 |
Description | IC-ANMBES 2021 Conference |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Supporters |
Results and Impact | IC-ANMBES 2021 Conference, Brasov, Romania: 2 - 4 June 2021. |
Year(s) Of Engagement Activity | 2021 |
Description | International Microfluidics Conference, London, October 2015 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other audiences |
Results and Impact | Microfluidics Conference, held in London over 2 days 20/21 October 2015. |
Year(s) Of Engagement Activity | 2015 |
Description | Invited Lecture |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other audiences |
Results and Impact | 7th European Joint Theoretical / Experimental Meeting on Membranes, Graz, 7th - 9th April 2021. Lecture given Online. |
Year(s) Of Engagement Activity | 2021 |
Description | Invited Lecture, Brasov, Romania |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Postgraduate students |
Results and Impact | Invited Lecture, IC-ANMBES 2020 conference, Brasov, Romania, 16th - 18th June 2020 |
Year(s) Of Engagement Activity | 2020 |
Description | Invited Lecture, Daejeon, March 2014 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other academic audiences (collaborators, peers etc.) |
Results and Impact | Halim Kusumaatmaja invited to present a talk at the British Council workshop on soft matter Disseminating and sharing scientific knowledge to a wide audience, encouraging networking and future collaboration |
Year(s) Of Engagement Activity | 2014 |
Description | Invited Lecture, El Escorial, Spain |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Postgraduate students |
Results and Impact | Invited Lecture, Biophysics Summer School, El Escorial, Spain, 18th - 20th July 2019. |
Year(s) Of Engagement Activity | 2019 |
Description | Invited Lecture, Graz |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Postgraduate students |
Results and Impact | Invited Lecture, 7th European Joint Theoretical / Experimental Meeting on Membranes, Graz, 6th - 8th May 2020. |
Year(s) Of Engagement Activity | 2020 |
Description | Invited Lecture, Leeds |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Postgraduate students |
Results and Impact | Invited Lecture, Workshop on Lyotropic Liquid Crystals (Satellite to Annual British Liquid Crystal Conference), Leeds, 15th - 17th April 2019. |
Year(s) Of Engagement Activity | 2019 |
Description | Invited Lecture, Lund University |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Postgraduate students |
Results and Impact | Invited Lecture, Lund University, LINX Mini-Symposium, 14th March 2019. |
Year(s) Of Engagement Activity | 2019 |
Description | Invited Plenary Lecture - Dynamics in Biological AFM, AFM Conference and Workshop, |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other audiences |
Results and Impact | Requimte AFM Conference and Workshop, University of Porto, Portugal. |
Year(s) Of Engagement Activity | 2016 |
Description | Invited lecture and workshop, Julich, October 2014 |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other academic audiences (collaborators, peers etc.) |
Results and Impact | Pietro Cicuta was invited to present a student lecture and workshop on the subject of particles interactions with membranes. The event engaged and sparked discussion and encouraged future collaborative activity Engaged and sparked discussion, encouraged future collaborative activity |
Year(s) Of Engagement Activity | 2014 |
URL | http://www.fz-juelich.de/ics/ics-2/EN/Leistungen/ConferencesAndWorkshops/Particles@Membranes/Venue/_... |
Description | Invited lecture, Austria, August 2013 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Other academic audiences (collaborators, peers etc.) |
Results and Impact | Simon Connell invited to present a talk on Critical Membranes at the Bio-Nano summer school in Austria on the 14th August 2013. Disseminating and sharing scientific knowledge to a wide audience, encouraging networking and future collaboration |
Year(s) Of Engagement Activity | 2013 |
Description | Invited lecture, Austria, August 2014 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other academic audiences (collaborators, peers etc.) |
Results and Impact | Simon Connell presented a talk entitled 'Membrane Biophysics' at a Bio-Nano summer school in Austria in August 2014 Disseminating and sharing scientific knowledge to a wide audience, encouraging networking and future collaboration |
Year(s) Of Engagement Activity | 2014 |
Description | Invited lecture, Coventry, September 2014 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Public/other audiences |
Results and Impact | Simon Connell invited to present a talk on Fast Scan AFM dynamics in lipid membranes at the Bruker Nanoelectrical and Chemical workshop in Coventry on the 25th September 2014. Disseminating and sharing scientific knowledge to a wide audience, encouraging networking and future collaboration |
Year(s) Of Engagement Activity | 2014 |
Description | Invited lecture, Durham, August 2014 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Other academic audiences (collaborators, peers etc.) |
Results and Impact | Halim Kusumaatmaja was invited to present a talk at the 2014 Energy Landscapes symposium and workshop hosted at Durham University. Disseminating and sharing scientific knowledge to a wide audience, encouraging networking and future collaboration |
Year(s) Of Engagement Activity | 2014 |
Description | Invited lecture, Durham, December 2013 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Public/other audiences |
Results and Impact | John Sanderson invited to a Cafe Scientifique talk about the chemistry of biological membranes. The talk increased awareness, engagement and discussion around this topic. Knowledge transfer, networking and potential for future collaborative activity |
Year(s) Of Engagement Activity | 2013 |
Description | Invited lecture, Goettingen, November 2014 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Other academic audiences (collaborators, peers etc.) |
Results and Impact | Halim Kusumaatmaja was invited to present a talk at the Max Plank Institute for Dynamics and and Self Organisation in Goettingen in November 2014. Disseminating and sharing scientific knowledge to a wide audience, encouraging networking and future collaboration |
Year(s) Of Engagement Activity | 2014 |
Description | Invited lecture, London, September 2012 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other academic audiences (collaborators, peers etc.) |
Results and Impact | Simon Connell invited to present a talk on Critical fluctuations in Lipid Membranes at Faraday Discussions 160, on 11th September 2012 Disseminating and sharing scientific knowledge to a wide audience, encouraging networking and future collaboration |
Year(s) Of Engagement Activity | 2012 |
Description | Invited lecture, Lyon, September 2013 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Other academic audiences (collaborators, peers etc.) |
Results and Impact | Halim Kusumaatmaja invited to present a talk on the influence of interfacial interactions on structural and rheological properties of confined liquids in Lyon in September 2013. Disseminating and sharing scientific knowledge to a wide audience, encouraging networking and future collaboration |
Year(s) Of Engagement Activity | 2013 |
Description | Invited lecture, Toronto, September 2014 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other academic audiences (collaborators, peers etc.) |
Results and Impact | SImon Connell invited to present a talk on fast scan dynamics in lipid membranes at SPM on SPM in Toronto on the 5th September 2014 Disseminating and sharing scientific knowledge to a wide audience, encouraging networking and future collaboration |
Year(s) Of Engagement Activity | 2014 |
Description | Invited lecture, York, March 2014 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Other academic audiences (collaborators, peers etc.) |
Results and Impact | John Seddon invited present a lecture at the Bruker Poster Prize Day at the Unviersity of York on the 20th March 2014. Disseminating and sharing scientific knowledge to a wide audience, encouraging networking and future collaboration |
Year(s) Of Engagement Activity | 2014 |
Description | Invited workshop contributor, London, February 2014 |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other academic audiences (collaborators, peers etc.) |
Results and Impact | John Seddon was an invited participant at an EPSRC Physical Sciences leaders workshop at Imperial College London in February 2014. Disseminating and sharing scientific knowledge to a wide audience, encouraging networking and future collaboration |
Year(s) Of Engagement Activity | 2014 |
Description | John Sanderson, Chair - Membrane Peptides and Toxins |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other audiences |
Results and Impact | Seminars: Membrane Peptides and Toxins (Session Chair, Biophysical Society Annual Meeting, New Orleans, February 11 - 15, 2017. |
Year(s) Of Engagement Activity | 2017 |
Description | Meeting with the Chair of RSC Faraday Standing Committee on Conferences |
Form Of Engagement Activity | A formal working group, expert panel or dialogue |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Policymakers/politicians |
Results and Impact | Meetings with the Chair of RSC Faraday Standing Committee on Conferences |
Year(s) Of Engagement Activity | 2017,2018,2019,2020 |
Description | Meeting with the Co-Chair, EPSRC Physical Sciences Grants Meeting |
Form Of Engagement Activity | A formal working group, expert panel or dialogue |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Policymakers/politicians |
Results and Impact | , EPSRC Physical Sciences Grants Meeting (5th December 2018); |
Year(s) Of Engagement Activity | 2018 |
Description | Meeting with the Secretary, European Biophysics Societies' Association |
Form Of Engagement Activity | A formal working group, expert panel or dialogue |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Study participants or study members |
Results and Impact | Meeting with the Secretary, European Biophysics Societies' Association |
Year(s) Of Engagement Activity | 2013,2014,2015,2016,2017,2018,2019,2020 |
Description | Membranes Conference - EJTEMM |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Study participants or study members |
Results and Impact | EJTEMM Membranes Conference, Graz, 7-9 April 2021 |
Year(s) Of Engagement Activity | 2021 |
Description | One Life: Science At The Interface, September 2015 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Other audiences |
Results and Impact | One Life: Science At The Interface, September 2015 |
Year(s) Of Engagement Activity | 2015 |
Description | Oral presentation, Lancaster, July 2014 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Postgraduate students |
Results and Impact | Simon Connell presented a lecture at the EPSRC Physics of Life summer school at Lancaster University on 18th July 2014. Disseminating and sharing scientific knowledge to a wide audience, encouraging networking and future collaboration |
Year(s) Of Engagement Activity | 2014 |
Description | Oral presentation, Leeds, June 2013 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other academic audiences (collaborators, peers etc.) |
Results and Impact | Simon Connell presented a talk at the Royal Microscopy Society in Leeds on 26th June 2013. The talk encouraged further questions and debate Disseminating and sharing scientific knowledge to a wide audience, encouraging networking and future collaboration. |
Year(s) Of Engagement Activity | 2013 |
Description | Oral presentation, London, September 2013 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other academic audiences (collaborators, peers etc.) |
Results and Impact | Nicola McCarthy was invited to present a talk at the European High Pressure Research Group international conference in London. Disseminating and sharing scientific knowledge to a wide audience, encouraging networking and future collaboration |
Year(s) Of Engagement Activity | 2013 |
Description | Oral presentation, Manchester, October 2012 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other academic audiences (collaborators, peers etc.) |
Results and Impact | Simon Connell presenteda talk on surface science of biologically important interfaces on the 18th October 2012. The event initiated further questions and discussion among the audience. Disseminating and sharing scientific knowledge to a wide audience, encouraging networking and future collaboration. |
Year(s) Of Engagement Activity | 2014 |
Description | Plenary Lecture, Université Paris Descartes |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Postgraduate students |
Results and Impact | Plenary Lecture, ECOF 16th European Conference on Organized Films, Université Paris Descartes, 9th - 12th July 2019. |
Year(s) Of Engagement Activity | 2019 |
Description | Poster: Drug Lipidation in Membranes (full author list: Hannah M. Britt, Vian S. Ismail, Jackie A. Mosely and John M. Sanderson) |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other audiences |
Results and Impact | Poster: Drug Lipidation in Membranes, Biophysical Society Annual Meeting, New Orleans, February 11 - 15, 2017 |
Year(s) Of Engagement Activity | 2017 |
Description | Poster: Drug Lipidation in Membranes (full author list: Hannah M. Britt, Vian S. Ismail, Jackie A. Mosely and John M. Sanderson) |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other audiences |
Results and Impact | Poster: Drug Lipidation in Membranes, Biophysical Society Annual Meeting, Los Angeles, February 27-March 2, 2016 |
Year(s) Of Engagement Activity | 2016 |
Description | Remote Review Panel |
Form Of Engagement Activity | A formal working group, expert panel or dialogue |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Study participants or study members |
Results and Impact | Remote Review Panel: ESRF, Grenoble, Beamline ID02; 2 - 3 November 2021. |
Year(s) Of Engagement Activity | 2021 |
Description | Royal Society Discussion Meeting on Cell Mimicry |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Supporters |
Results and Impact | Royal Society Discussion Meeting on Cell Mimicry, 7 - 8 Nov 2022, Royal Society London |
Year(s) Of Engagement Activity | 2022 |
Description | Scientific Committee and Session Chair |
Form Of Engagement Activity | A formal working group, expert panel or dialogue |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Study participants or study members |
Results and Impact | Scientific Committee and Session Chair: EBSA European Biophysics Congress, Vienna, 24 - 28 July 2021. Hybrid meeting. |
Year(s) Of Engagement Activity | 2021 |
Description | Seminar at Edinburgh University, Chemistry Department |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Postgraduate students |
Results and Impact | Seminar at the Dept of Chemistry, University of Edinburgh - 6 November 2019 |
Year(s) Of Engagement Activity | 2019 |
Description | Simon Connell Conference talk - Critical Dynamics in supported membranes |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other audiences |
Results and Impact | 8th-10th December 2016 "Critical Dynamics in supported membranes", Biological Interfaces - Interfacing with Biology, University of Leeds (International Conference) |
Year(s) Of Engagement Activity | 2016 |
Description | Simon Connell Conference talk - Fast Scan AFM of lipid dynamics |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Other audiences |
Results and Impact | 13-15th December 2016, "Fast Scan AFM of lipid dynamics" UK SPM Conference, Department of Materials Science and Metallurgy, Cambridge University. |
Year(s) Of Engagement Activity | 2016 |
Description | Simon Connell Conference talk - Fluctuating Lipid Nanodomains near Critical Transitions |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other audiences |
Results and Impact | 4th-8th July 2016 "Fluctuating Lipid Nanodomains near Critical Transitions", Complexity in the Chemistry and Physics of lipid membranes, Telluride TSRC Workshop, Telluride , Colorado, USA. |
Year(s) Of Engagement Activity | 2016 |
Description | Simon Connell Conference talk - Quantifying lipid membrane dynamics using Fast Scan AFM |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other audiences |
Results and Impact | 26th-30th August 2016 "Quantifying lipid membrane dynamics using Fast Scan AFM", SPM-on-SPM, Changchun, China |
Year(s) Of Engagement Activity | 2016 |
Description | Simon Connell seminar talk - AFM Studies of Bilayer Dynamics, Structure and Growth in the Three-Phase Region |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other audiences |
Results and Impact | 13th February 2015, "AFM Studies of Bilayer Dynamics, Structure and Growth in the Three-Phase Region", Departmental Seminar, Olmsted Group, Georgetown University, USA. |
Year(s) Of Engagement Activity | 2015 |
Description | Simon Connell seminar talk - Critical Fluctuations in Lipid Bilayers |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Other audiences |
Results and Impact | 27th October 2016 "Critical Fluctuations in Lipid Bilayers", Theoretical Physics Group Seminar, Department of Physics, University of Warwick |
Year(s) Of Engagement Activity | 2016 |
Description | Simon Connell seminar talk - Lipid Nanodomain Dynamics: Fast AFM. Line tensions, Ising Models and coupling Modes |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Other audiences |
Results and Impact | 8th Feb 2017, "Lipid Nanodomain Dynamics: Fast AFM. Line tensions, Ising Models and coupling Modes", London School of Nanotechnology (LCN) Colloquium. |
Year(s) Of Engagement Activity | 2017 |
Description | Simon Connell seminar talk - Membranes, Microfluidics and Microbubbles: from fundamentals to clinical application |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Other audiences |
Results and Impact | 13th April 2015 "Membranes, Microfluidics and Microbubbles: from fundamentals to clinical application", Interdisciplinary Computing and Complex BioSystems Research Group, School of Computing Science, University of Newcastle |
Year(s) Of Engagement Activity | 2015 |
Description | Simon Connell seminar talk - Structure, Dynamics and Mechanics of Three-Phase Lipid Bilayers |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other audiences |
Results and Impact | 16th February 2015, "Structure, Dynamics and Mechanics of Three-Phase Lipid Bilayers", Departmental Seminar, Dufresne Group, Yale University, USA. |
Year(s) Of Engagement Activity | 2015 |
Description | Simon Connell seminar talk - Structure, Dynamics and Mechanics of Three-Phase Lipid Bilayers |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other audiences |
Results and Impact | 13th March 2015, "Structure, Dynamics and Mechanics of Three-Phase Lipid Bilayers", Departmental Seminar, Ando Group, Kanazawa University, Japan, |
Year(s) Of Engagement Activity | 2015 |
Description | SoftComp Annual meeting |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other audiences |
Results and Impact | Presentation in Ancona, Italy at the SoftComp annual meeting - Demixing transitions in Bicontinuous Cubic Phases close to the critical point |
Year(s) Of Engagement Activity | 2016 |
Description | StatPhys 26 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other audiences |
Results and Impact | Talk at StatPhys, Lyon, France - Demixing transitions in Bicontinuous Cubic Phases close to the critical point |
Year(s) Of Engagement Activity | 2016 |
Description | Vesicle Carriers Symposium, Manchester, March 2016 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Other audiences |
Results and Impact | Presented talk at the University of Manchester on Vesicle Carriers. The symposium was spread over 2 days - 4th and 5th March 2016 and was fully attended. |
Year(s) Of Engagement Activity | 2016 |
Description | Zing Computational Chemical Biology Conference, 6-10 August 2015 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other audiences |
Results and Impact | Zing Computational Chemical Biology conference, invited delegates from a worldwide audience, held over the 6-10 August 2015. |
Year(s) Of Engagement Activity | 2015 |