NISA: Novel approaches for in situ analysis of biomolecules

Lead Research Organisation: University of Birmingham
Department Name: Sch of Biosciences

Abstract

The aim of the research is to develop novel approaches for the analysis of biomolecules, and in particular proteins, directly from their natural (or actual) environment, i.e., to develop approaches for in situ biomolecular analysis. Proteins are the work-horses of the cell and perform all the functions required for life. They also find uses as therapeutics and in consumer products. To gain insight into the various and specific roles of proteins in life processes, or to determine the therapeutic efficacy of protein drugs, or to establish the environmental fate of protein additives in consumer products, it is necessary to be able to analyse proteins at a molecular level. Mass spectrometry, in which ionised molecules are characterised according to their mass-to-charge, is ideally suited to this challenge, offering high sensitivity, broad specificity (all molecules have a mass), and the capability for chemical structure elucidation. The ultimate goal is to link molecular analysis directly to molecular environment. Much like a forensics officer tasked with determining the presence of an illicit substance, there is much greater reliability and credibility afforded to an analysis performed at the scene of the crime than to one performed following removal of the sample to a separate location and alternative surroundings.

Growing evidence suggests in situ protein analysis has groundbreaking roles to play in biomarker discovery, diagnosis & early detection of disease, targeting therapeutics (personalised medicine) and assessment of therapeutic efficacy. The benefits of in situ protein analysis can be illustrated by considering a thin tissue section through a drug-treated tumour. In principle, in situ analysis would inform on drug-target interactions (i.e., is the drug binding to the correct protein?). Moreover, with in situ protein analysis the capacity for artefact introduction as a result of sample preparation (e.g., application of a matrix) or sample damage is eliminated.

Nevertheless, a number of challenges exist. Proteins are large molecules associated with a vast array of chemical modifications, and which form loosely-bound complexes with themselves, other proteins and other molecule types. It is not only their chemical structure but also their overall 3-D structure which dictate their function. Other molecular classes that are hugely important in biological processes also have an intricate relationship with proteins. Any in situ mass spectrometry approach needs to be able to meet these analyte-driven challenges, i.e., it must be capable of (a) measuring proteins and characterising any modifications, (b) detecting protein complexes and determining their constituents, (c) providing information on 3-D structure, and (d) detecting other relevant molecular classes. Moreover, there are technique-driven challenges for in situ analysis including inherently high sample complexity and wide ranging concentrations, and opportunities for quantitation.

The research will meet these challenges by developing a newly emerging in situ approach, liquid extraction surface analysis mass spectrometry, in combination with two complementary types of ion mobility spectrometry (which can either provide information on 3-D structure, or separate ionised molecules in the mass spectrometer on the basis of their 3-D shape) and a structural elucidation strategy known as electron-mediated dissociation mass spectrometry.
The research will be undertaken primarily at the University of Birmingham in the Advanced Mass Spectrometry Facility in the School of Biosciences and the School of Chemistry mass spectrometry facility. The programme involves a number of academic and industrial collaborators and additional research will be carried out during scientific visits to National Physical Laboratory (NPL), Thermo Fisher Scientific, Waters, Owlstone, Florida State University, Texas A&M University and Université d'Aix-Marseille.

Planned Impact

Who will benefit from this research?

The beneficiaries of this research will be analytical instrument manufacturers, the pharmaceutical industry, the healthcare industry, the NHS and its patients, and one of the UK's National Measurement Institutes (the National Physical Laboratory, NPL).

How will they benefit from this research?

The proposed research has the potential to contribute significantly to the nation's health and wealth:

Analytical instrumentation manufacturers: The work will enable the development of new instrumentation and workflows which can be employed in a broad range of new applications. The 2006 Royal Society of Chemistry Analytical and Measurement Sciences Report reported the UK analytical sector has an annual turnover of £7 billion and that the worldwide laboratory and analysis market was $25 billion. Thermo Fisher Scientific, Waters Corp. and Advion Inc are global companies, all of whom have a strong base in the UK. The proposed work will lead to increased sales thus fostering global economic performance and the economic competitiveness of the UK. Owlstone Ltd. is an SME (small and medium-sized enterprise), established in 2004, developing instruments based on the FAIMS technology. Again, the research will lead to increased sales and thus UK economic competitiveness.

National Measurement Institute: The proposed research will facilitate the UK's national measurement institute NPL, project partner on the fellowship, in achieving its goal of providing 'world-leading measurement solutions which are critical to commercial research and developments and supporting business across the UK and the globe'. NPL is a world leader in mass spectrometry imaging with the largest worldwide MSI research group and a recently established National Centre of Excellence in Mass Spectrometry Imaging (NiCE-MSI). In the first year of the NiCE-MSI, NPL delivered £100K of commercial consultancy to UK pharma. The proposed research will increase this through the develment of extra capabilities.

Pharmaceutical industry: The pharmaceutical industry (annual turnover £31.8 bn) is underpinned by analytical science. The proposed research will facilitate the development of new drugs by providing methods for determining drug distribution and associated effects on tissue biochemistry, drug-target engagement and efficacy. AstraZeneca, a global company with a UK base, are partners on the programme meaning the research will have a direct impact on the health and wealth of the nation.

Healthcare industry: The healthcare industry is similarly underpinned by analytical science. The proposed research has direct implications for safety and quality assurance and will feed into the PI's ongoing collaboration with Unilever, a global company with a UK base. Again, the benefits are improved health and increased wealth of the nation.

NHS and its patients: The annual cost to the UK of periodontitis is £2.8 billion, for rheumatoid arthritis is £4 billion and for cancer is greater than £15 billion. Annual costs for kidney dialysis account for 2% of the NHS budget. In each case, there is a need for new analytical approaches for determining risk factors and for monitoring disease onset, progression and therapeutic response. The proposed research addresses that need and therefore has the potential to both reduce costs and improve health. Similarly, screening for haemoglobinopathies and in-born errors of metabolism is carried out for ~700,000 newborns every year in the UK. The combined estimate for Europe and the US is 8 million tests annually. The benefits of the proposed research would be reduced cost per test and improved diagnosis, and the research is supported by Birmingham Children's Hospital - the largest newborn screening centre in the UK.

Publications

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Cooper HJ (2016) To What Extent is FAIMS Beneficial in the Analysis of Proteins? in Journal of the American Society for Mass Spectrometry

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Creese AJ (2016) Separation of cis and trans Isomers of Polyproline by FAIMS Mass Spectrometry. in Journal of the American Society for Mass Spectrometry

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Griffiths R (2019) Native mass spectrometry imaging of intact proteins and protein complexes in thin tissue sections in International Journal of Mass Spectrometry

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Griffiths RL (2018) Ambient surface mass spectrometry-ion mobility spectrometry of intact proteins. in Current opinion in chemical biology

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Kocurek KI (2018) Ambient ionisation mass spectrometry for in situ analysis of intact proteins. in Journal of mass spectrometry : JMS

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Kocurek KI (2017) Top-Down LESA Mass Spectrometry Protein Analysis of Gram-Positive and Gram-Negative Bacteria. in Journal of the American Society for Mass Spectrometry

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Lopez-Clavijo A (2015) Electron capture dissociation mass spectrometry of phosphopeptides: Arginine and phosphoserine in International Journal of Mass Spectrometry

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Powell T (2017) Subcritical Water Hydrolysis of Peptides: Amino Acid Side-Chain Modifications. in Journal of the American Society for Mass Spectrometry

 
Description This grant started on 1st June 2014. In our first ResearchFish submission we reported that we had published three papers. The first shows that we can directly analyse proteins implicated in non-alcoholic liver disease directly from liver tissue. In the second, we show that direct surface sampling coupled with high resolution mass spectrometry may be applied to the analysis of intact proteins directly from live bacterial colonies growing on agar plates. The third paper is a review on the analysis of proteins from dried blood spots, which was commissioned by the editors of Expert Reviews in Proteomics.
In the second recording period, we delivered a further ten publications. These include 8 original research publications. In the first we demonstrate novel methods for the mapping of glycosylation in the bacteria Campylobacter jejuni. In the second, we combine ion mobility, electron capture dissociation and molecular dynamics simulations to understand the chemistry of phosphopeptides. A third paper extends that work. Two papers focus on native mass spectrometry of protein complexes directly from surfaces - a grand challenge for our research programme. Two papers focus on the coupling of FAIMS with direct surface sampling, another step-change for our research. One paper describes the application of FAIMS to phosphoproteomics. One is a book chapter detailing the protocols we have developed for FAIMS phosphoproteomics, which was commisioned by the editors of Methods in Molecular Biology. One is a Critical Insight on FAIMS of proteins invited by the Editor of the Journal of the American Society for Mass Spectrometry.
In the third recording period, we delivered a further seven papers. One focuses on native LESA mass spectrometry, showing the approach is suitable for complexes up to 800 kDa. We have demonstrated LESA FAIMS mass spectrometry imaging of thin tissue sections, and have combined LESA with MALDI in order to interrogate the fundamentals of LESA. We have demonstrated sub-critical water as a medium for proteolysis in proteomics, and applied ETD to the analysis of phosphopeptides in plants. We have demonstrated that FAIMS is capable of separating cis/trans isomers of polyproline peptides. We have also published a book chapter on the post-translational modification citrullination. In the fourth recording period, we have delivered four original research papers and one review on ambient surface ion mobility mass spectrometry of proteins. We have demonstrated native LESA mass spectrometry imaging of proteins and protein complexes in thin tissue sections, and raster-mode continuous flow liquid microjunction sampling imaging of proteins in tissue. We have demonstrated LESA mass spectrometry of a range of gram positive and gram negative bacteria, and probed the amino acid side chain modifications induced by sub critical water. In the latest recording period, we have published three original research papers, one on the use of FAIMS in dried blood spot proteomics, one on LESA EID of small molecule drugs from tissue, and one on multistep FAIMS mass spectrometry imaging. We have also published a review of ambient ionisation mass spectrometry, We have four original research manuscripts under review, plus two submitted review manuscripts.
Exploitation Route We have demonstrated that liquid extraction surface analysis mass spectrometry may be used for the analysis of intact proteins in a range of complex biological matrices, and shown that it can be coupled with FAIMS bringing a range of benefits. Furthermore, we have demonstrated that we can perform native LESA mass spectrometry. That is, we can sample protein complexes directly from surfaces including tissue sections. Both of these findings constitute step-changes for in situ analysis of biomolecules.
Sectors Agriculture, Food and Drink,Healthcare,Pharmaceuticals and Medical Biotechnology

URL http://www.biosciences-labs.bham.ac.uk/cooper/nisa.html
 
Description The grant started on 1 June 2014. Twenty original research papers have been published, in addition to one invited opinion piece, two reviews and two book chapters. Software for the visualisation of FAIMS data has been developed and made freely available via the research group website. Software for the generation of ion images from native LESA mass spectrometry imaging data has been developed and made freely available via the research group website. Since the last submission period, four original research papers have been published together with two review papers. A further four manuscripts, plus one review, are currently submitted and under review. Two grants have been funded.
First Year Of Impact 2014
 
Description EPSRC Responsive mode
Amount £411,000 (GBP)
Funding ID EP/R018367/1 
Organisation Engineering and Physical Sciences Research Council (EPSRC) 
Sector Academic/University
Country United Kingdom
Start 04/2018 
End 03/2021
 
Description Established Career Fellowship Extension
Amount £1,200,000 (GBP)
Funding ID EP/S002979/1 
Organisation Engineering and Physical Sciences Research Council (EPSRC) 
Sector Academic/University
Country United Kingdom
Start 06/2019 
End 05/2022
 
Description National Productivity Industrial Fund studentship
Amount £1 (GBP)
Organisation Engineering and Physical Sciences Research Council (EPSRC) 
Sector Academic/University
Country United Kingdom
Start 10/2017 
End 09/2021
 
Title FAIMS software 
Description Software for generating total ion transmission maps and single ion transmission maps for the analysis of FAIMS data. 
Type Of Material Data analysis technique 
Year Produced 2015 
Provided To Others? Yes  
Impact The software is freely available on our group website. 
URL http://www.biosciences-labs.bham.ac.uk/cooper/software.html
 
Title Native mass spectrometry imaging software 
Description Visualisation of native mass spectrometry imaging data 
Type Of Material Data analysis technique 
Year Produced 2017 
Provided To Others? Yes  
Impact The software enables the generation of ion images from native LESA mass spectrometry imaging data. the software is published as supplementary material in the research paper 10.1016/j.ijms.2017.10.009. It is also available for download from the group website. 
URL http://www.biosciences-labs.bham.ac.uk/cooper/software.php
 
Description FIU_Fernandez-Lima 
Organisation Florida International University (FIU)
Country United States 
Sector Academic/University 
PI Contribution Expertise, staff training, access to equipment.
Collaborator Contribution Expertise, staff training, access to TIMS equipment.
Impact Manuscript submitted and conference abstract submitted.
Start Year 2018
 
Description NPL AZ 
Organisation AstraZeneca
Country United Kingdom 
Sector Private 
PI Contribution This partnership match funds two PhD students (Alexander Dexter and Elizabeth Randall) in my laboratory. The students are principally funded through the EPSRC PSIBS DTC.
Collaborator Contribution Both NPL and AZ are providing a direct financial contribution (total of 20,000 GBP per student), although this contribution is supplied via NPL alone. AZ have provided drug treated tissue for method development.
Impact The collaboration has only just been agreed. There are no outputs as yet.
Start Year 2014
 
Description NPL AZ 
Organisation National Physical Laboratory
Country United Kingdom 
Sector Academic/University 
PI Contribution This partnership match funds two PhD students (Alexander Dexter and Elizabeth Randall) in my laboratory. The students are principally funded through the EPSRC PSIBS DTC.
Collaborator Contribution Both NPL and AZ are providing a direct financial contribution (total of 20,000 GBP per student), although this contribution is supplied via NPL alone. AZ have provided drug treated tissue for method development.
Impact The collaboration has only just been agreed. There are no outputs as yet.
Start Year 2014
 
Description NPL_Klaudia_Kocurek 
Organisation National Physical Laboratory
Country United Kingdom 
Sector Academic/University 
PI Contribution This partnership match funds a PhD students (Klaudia Kocurek) in my laboratory. The student is principally funded through the EPSRC DTA.
Collaborator Contribution NPL are providing a direct financial contribution (total of 22,500 GBP ). The student will spend 6 months at NPL during her sceond year and will have access to state of the art mass spectrometry imaging and metrology equipment.
Impact None as yet. The student (Klaudia Kocurek) began her studies in Oct 2015.
Start Year 2015
 
Description Oslo_CR 
Organisation University of Oslo
Country Norway 
Sector Academic/University 
PI Contribution Hosted PhD student Cecilie Rosting for 3 months research placement. Research focused on FAIMS for dried blood spot proteomics.
Collaborator Contribution Sponsorship of PhD student's visit.
Impact None as yet.
Start Year 2016
 
Description Surrey_Bailey 
Organisation University of Surrey
Department Department of Chemistry
Country United Kingdom 
Sector Academic/University 
PI Contribution Expertise, staff training, access to mass spectrometry equipment.
Collaborator Contribution Staff training, access to DAPNe equipment
Impact None as yet
Start Year 2018
 
Description TAMU_Anna_Simmonds 
Organisation Texas A&M University
Department Department of Chemistry
Country United States 
Sector Academic/University 
PI Contribution Mass spectrometry and ion mobility spectrometry of phosphopeptides
Collaborator Contribution Molecular dynamics simulations
Impact No outputs yet as collaboration has just started.
Start Year 2017
 
Description Thermo_FAIMS 
Organisation Thermo Fisher Scientific
Country United States 
Sector Private 
PI Contribution Expertise, intellectual input, samples
Collaborator Contribution Access to equipment
Impact Manuscript in preparation, conference presentation
Start Year 2017
 
Description Thermo_UHMR 
Organisation Thermo Fisher Scientific
Country United States 
Sector Private 
PI Contribution Expertise, intellectual input, samples
Collaborator Contribution Access to instrumentation
Impact Manuscript submitted, conference presentation
Start Year 2018
 
Description UCB_Emma_Sisley 
Organisation UCB Pharma
Country United Kingdom 
Sector Private 
PI Contribution The aim of the project is to develop and apply liquid extraction surface analysis (LESA) mass spectrometry to understand the pathogenesis of fibrosis. This is a studentship project for a PhD part funded by EPSRC DTCC 'Physical Sciences for Health' and part-funded by UCB.
Collaborator Contribution Part funded studentship. Supply of tissue from animal models.
Impact No outputs yet as collaboration has just started.
Start Year 2017
 
Description UCL_KIK 
Organisation University College London
Department Eastman Dental Institute
Country United Kingdom 
Sector Academic/University 
PI Contribution LESA analysis of Bacillus isolates
Collaborator Contribution Supply of Bacillus isolates
Impact None as yet - new collaboration.
Start Year 2016
 
Description Waters_Claude 
Organisation Waters Corporation
Department Waters Corporation Centre of Mass Spectrometry Excellence
Country United Kingdom 
Sector Private 
PI Contribution Tissue samples
Collaborator Contribution Access to DESI equipment
Impact Manuscript submitted, conference presentations
Start Year 2018
 
Description Waters_Marsden-Edwards 
Organisation Waters Corporation
Department Waters Corporation Centre of Mass Spectrometry Excellence
Country United Kingdom 
Sector Private 
PI Contribution Tissue samples
Collaborator Contribution Access to mass spectrometry equipment
Impact None as yet
Start Year 2018
 
Description Agewell Conference 2016 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Study participants or study members
Results and Impact PhD student Elliott Palmer advertised his research to the 1000 elders cohort in the UoB medical school to assess future opportunities available for the application of his research.
Year(s) Of Engagement Activity 2016
 
Description Blue Coat School outreach 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Schools
Results and Impact Postdoctoral researcher Dr Andrea Lopez-Clavijo gave an outreach talk at Blue Coat secondary school, Coventry.
Year(s) Of Engagement Activity 2016
 
Description FAIMS workshop 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact PDRA organised and hosted evening workshop on FAIMS at the annual American Society for Mass Spectrometry conference. Audience ~200.
Year(s) Of Engagement Activity 2018
 
Description FameLab 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Doctoral students Elizabeth Randall and Thomas Powell have entered FameLab, a communications competition designed to engage and entertain by breaking down science, technology and engineering concepts into three minute presentations. They are both currently through to the Regional Finals to be held in Manchester.
Year(s) Of Engagement Activity 2011,2016
URL http://www.cheltenhamfestivals.com/about/famelab/famelab-uk/
 
Description Images of Research 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Doctoral researcher Elizabeth Randall presented her research as a single image which was displayed in a gallery at the Birmingham museum and art gallery, then discussed research with members of general public, who were visitors to gallery. Audience: general public, audience size: approx. 70. Impact: improving explanation of research as well as disseminating information.
Year(s) Of Engagement Activity 2015
URL https://www.flickr.com/photos/70731382@N04/sets/72157651098140939
 
Description Knowledge transfer workshop 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact A one day knowledge transfer workshop "New Advances in Biological Mass Spectrometry, ~70 attendees from academia and industry, held at University of Birmingham.
Year(s) Of Engagement Activity 2019
 
Description Meet the Scientists at ThinkTank Birmingham 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Schools
Results and Impact Small scale scientific experiments were designed for primary age school children to take part in at Birmingham's science museum ThinkTank. The event took place during half term holidays and was attended by several hundred children and their parents. PhD students Emma Sisley, Anna Simmonds, Jana Havlikova and James Hughes were involved in delivering the event.
Year(s) Of Engagement Activity 2017
 
Description NPL Academy 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Schools
Results and Impact Doctoral researcher Elizabeth Randall was involved in supervising GCSE level pupils in a week-long research project at NPL. audience: secondary school pupils (x2), their teachers and parents.
Year(s) Of Engagement Activity 2015
URL http://www.npl.co.uk/educate-explore/academy/
 
Description New Advances in Biological Mass Spectrometry Workshop 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Other audiences
Results and Impact Approximately 70 people attended a public engagement workshop aimed at informing on the latest advances in mass spectrometry. Attendees were researchers who would not normally make use of mass spectrometry in their research. It was also attended by delegates from industry. At least one new collaboration has arisen from the event.
Year(s) Of Engagement Activity 2016
 
Description Public engagement training 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Postgraduate students
Results and Impact PhD student Elizabeth Randall received training from BIG (British Interactive Group) STEM communicator's network, in how to train researchers in public engagement. Then designed and delivered training courses for early career researchers at UoB in the skills required for public engagement - communication, use of accessible language etc.
Year(s) Of Engagement Activity 2016
 
Description Royal International Air Tatoo 2017 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Schools
Results and Impact PhD student Klaudia Kocurek manned an exhibition stand for NPL at the Royal International Air Tattoo 2017 air show in Gloucestershire. This included an interactive display of some basics of measurement and general technology to encourage school children to go into STEM fields. The stand was accessible to everyone at the air show (~160,000 people from all over the UK) but aimed primarily at a younger audience. The stand included a demonstration of 3-D printing set up (printing tiny representations of SI units that could be taken away by members of the public), along with 'dolls of confusion' (a set of wooden dolls of different sizes and weights-the smallest one being the heaviest-to fool people's perception of mass and demonstrate the advantage of using a balance ) and a thermal camera, plus booklets on the SI units.
Year(s) Of Engagement Activity 2017
 
Description SET for Britain 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Policymakers/politicians
Results and Impact Poster competition at Houses of Parliament entered by doctoral researcher Elizabeth Randall. Audience: politicians and prominent figures in science/technology industry. Main impact: raise awareness of research areas in UK, especially those undertaken by early career researchers (with a view to engaging politicians in discussion with scientists for informing/ influencing policy)
Year(s) Of Engagement Activity 2015
URL http://www.setforbritain.org.uk/index.asp
 
Description School Careers Event 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact PDRA attended school careers fair to discuss careers in academia to year groups 8-13.
Year(s) Of Engagement Activity 2018
 
Description School presentation 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Schools
Results and Impact The PI (Helen Cooper) visited Sutton Girls Grammar School, Sutton Coldfield, to give a talk on the NISA research and careers in academia, to around 70 sixth form students and their parents. The talk was followed by a Q&A session.
Year(s) Of Engagement Activity 2016
 
Description School visit 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Schools
Results and Impact A 30 minute presentation delivered to 6th form students at Nether Stowe School describing career trajectory (choice of degree, subsequent choices and PhD)
Year(s) Of Engagement Activity 2018
 
Description Schools outreach 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Schools
Results and Impact Dr Rian Griffiths visited Woodrush Community High School to give a talk on NISA research. The presentation was followed by a Q&A session.
Year(s) Of Engagement Activity 2015
 
Description Schools outreach 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Schools
Results and Impact The PI (Helen Cooper) visited King Edward VI College, Totnes, Devon, and gave a talk on the NISA research, and careers in academia, to approximately 20 sixth form students. The talk was followed by a Q&A session.
Year(s) Of Engagement Activity 2015
 
Description Schools outreach 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Schools
Results and Impact Doctoral researcher Elizabeth Randall visited Altrincham Girls' Grammar School to lead small group discussions about how chemistry is used specifically in her research, together with more general careers advice. Audience: GCSE/A-Level pupils, chemistry teachers, audience size: approx. 30 pupils, 3 teachers.
Impact: raise profile of research group and academic careers, improve confidence in STEM subjects at girls' school.
Year(s) Of Engagement Activity 2015
 
Description Storytelling researcher video competition 
Form Of Engagement Activity A broadcast e.g. TV/radio/film/podcast (other than news/press)
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact Doctoral researcher Elizabeth Randall entered the University of Birmingham Think: Public Engagment 'Storytelling researcher video competition' (https://thinkpe.wordpress.com/2015/09/17/storytelling-researcher-video-competition/) with a video explaining some of the NISA research.
Year(s) Of Engagement Activity 2015
URL https://www.youtube.com/watch?v=xRRsKPvwAiA
 
Description The Brilliant Club 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Schools
Results and Impact PhD student Elizabeth Randall designed and delivered a course of tutorials to 6th form school pupils (The Holte School, Birmingham) on the topic of mass spectrometry imaging in forensic science and a further course on 'the healthy heart' to primary school pupils (St Mary and St Benedict's Catholic Primary School).
Year(s) Of Engagement Activity 2016
 
Description Think Corner 2014 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Think Corner was a 'pop-up shop' organised by University of Birmingham over two weeks in the Pallasades shopping centre in Birmingham. The aim was to disseminate information about research being undertaken at the University. Rian Griffiths (PDRA) organised a stall with a hands on activity to explain the structure of proteins and methods for determining protein structure.

Public dissemination
Year(s) Of Engagement Activity 2014
 
Description ThinkTank 2019 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact One day event at Thinktank Science Museum running small science experiments for children and young adults aged 5-15. Experiments include investigating capillary action in celery, visualising everyday objects under microscopy and learning about different length scales.
Year(s) Of Engagement Activity 2019
 
Description TransMed2017 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Postgraduate students
Results and Impact Transmed2017 was a two-day, student-led conference hosted by the University of Birmingham from 20-21st June 2017 that addressed the themes: nanomaterials and their effect on biological matter; analytical chemistry within a bio-medicinal setting; and the development of new imaging techniques, and computational and mathematical methods for medical devices through invited talks and posters from PG researchers from across the UK and two keynotes talks from Professor Mark Bagley (University of Sussex) and Professor Liam Grover (University of Birmingham). In addition, four invited speakers delivered 'enrichment sessions' on careers, communication and outreach.
Year(s) Of Engagement Activity 2017
URL https://www.birmingham.ac.uk/research/activity/sci-phy/news/Transmed-2017-A-summary.aspx
 
Description University of Birmingham Open Day 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Schools
Results and Impact Dr Rian Griffiths created an outreach poster on our research which was presented at the open day, leading to discussions with prospective students and general public.Prof Cooper was similarly involved with talking to general public and school students about research at the university.
Year(s) Of Engagement Activity 2017
 
Description University of Birmingham Research Poster Conference 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact University of Birmingham Research Poster Conference open to the general public. Poster given by PhD student Klaudia Kocurek.
Year(s) Of Engagement Activity 2016
 
Description Women in Science and Engineering (WISE) event 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Undergraduate students
Results and Impact Dr Rian Griffiths gave a talk about her journey into academia to an audience of undergraduate students in science and engineering based subjects (~20 people, mix of educational backgrounds), followed by questions and discussion. One of the attendees contacted her afterwards to ask for advice on ways to approach finding lab experience over the summer in an academic setting.
Year(s) Of Engagement Activity 2017
 
Description Woodrush High School Careers fair 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Schools
Results and Impact Postdoctoral researcher Dr Rian Griffiths attended the Woodrush High School Careers Fair to provide info on careers in research.
Year(s) Of Engagement Activity 2016