Colloidal Cell Delivery Systems
Lead Research Organisation:
University of Nottingham
Department Name: Sch of Pharmacy
Abstract
As stem cell technologies advance towards clinical application there is an urgent need to enhance the methods of delivering these cells into the body. Current methods of simple injection of cell suspensions are crude because they waste cells, compromise viability and generate poor starting conditions for the regeneration of a tissue. In this collaborative project between the universities of Nottingham and Manchester we will establish an injectable material consisting of a colloid mixture of cells and particles that is injectable at room temperature and aggregates into a porous colloidal gel at body temperature. This material could form the basis for cell delivery in neurology, endocrinology, hepatology, orthopaedics and many other areas because the components are well understood and trusted materials with long track records of use in the body. A successful outcome for this project will prove the principle of cell delivery into skeletal muscle tissue using temperature-triggered assembly and establish the fundamental principles involved.
Publications
Wang W
(2009)
Biodegradable Thermoresponsive Microparticle Dispersions for Injectable Cell Delivery Prepared Using a Single-Step Process
in Advanced Materials
Wang W
(2008)
Self-immolative polymers.
in Angewandte Chemie (International ed. in English)
Sicilia G
(2014)
Programmable polymer-DNA hydrogels with dual input and multiscale responses.
in Biomaterials science
Magnusson JP
(2008)
Ion-sensitive "isothermal" responsive polymers prepared in water.
in Journal of the American Chemical Society
Magnusson J
(2011)
Synthetic polymers for biopharmaceutical delivery
in Polym. Chem.
Hruschka V
(2015)
Evaluation of a Thermoresponsive Polycaprolactone Scaffold for In Vitro Three-Dimensional Stem Cell Differentiation
in Tissue Engineering Part A
Fraylich MR
(2010)
Thermally-triggered gelation of PLGA dispersions: towards an injectable colloidal cell delivery system.
in Journal of colloid and interface science
Fraylich M
(2008)
Poly(D,L-lactide-co-glycolide) dispersions containing pluronics: from particle preparation to temperature-triggered aggregation.
in Langmuir : the ACS journal of surfaces and colloids
Dey S
(2011)
Enzyme-passage free culture of mouse embryonic stem cells on thermo-responsive polymer surfaces
in Journal of Materials Chemistry
Cheikh Al Ghanami R
(2010)
Responsive particulate dispersions for reversible building and deconstruction of 3D cell environments
in Soft Matter
| Description | Self-assembling materials for tissue engineering and cell culture were developed. Several key papers were published showing that materials could be formulated with a number of cell types into free-flowing suspensions, then injected into sites at body temperature, whereupon the materials gelled to provide a support matrix for cell growth. Reduction of temperature allowed disassembly of the support and recovery of cells |
| Exploitation Route | Clinical expansion of stem cells for therapy A number of patents were filed and these have been licensed to a spin-out, RegenTec. Further work in this area is funded by the EPSRC Centre for Innovative Manufacture in Stem Cell Technologies. The PDRA on the project, Dr Wenxin Wang, is now a tenured senior lecturer at University College Dublin following a lecturer post at NUI Galway |
| Sectors | Chemicals,Healthcare,Pharmaceuticals and Medical Biotechnology |
| Description | Findings formed part of an application which led to successful establishment of EPSRC Centre for Innovative manufacture in Regenerative Medicine. In addition, patents have been licensed to RegenTec, now Locate Therapeutics, and a second PDRA, Dr Aram Saeed was appointed on to this latter grant. |
| First Year Of Impact | 2009 |
| Sector | Chemicals,Education,Manufacturing, including Industrial Biotechology,Pharmaceuticals and Medical Biotechnology |
| Impact Types | Economic,Policy & public services |
| Description | The University of Manchester |
| Organisation | University of Manchester |
| Country | United Kingdom |
| Sector | Academic/University |
| Start Year | 2007 |
| Title | POLYMER PARTICLES PREPARED FROM POLYMERISABLE ALKYLENE GLYCOL (METH) ACRYLATE MONOMERS |
| Description | The invention provides polymer particles that are obtainable by a method selected from emulsion methods, diffusion methods and evaporation methods carried out in the presence of surface-engineering surfactant which is one or more polymer that displaysa lower critical solution temperature,in aqueous media, that is between 10 to 90°C, this polymer being the polymerisation product of one or more monomer selected from polymerisable alkyleneglycol acrylate monomers and polymerisable alkyleneglycol methacrylate monomers. The polymer particles can be used in controlled release applications, such as flavour release applications, fragrance release applications and biomedical applications. The invention also provides a cell support matrix comprising the polymer particles. |
| IP Reference | WO2010043892 |
| Protection | Patent application published |
| Year Protection Granted | 2010 |
| Licensed | Yes |
| Impact | Significant interest from a number of other companies and a further project in the EPSRC Centre for Innovative Manufacture in Regemerative Medicine. |