Next Generation Analytical Tools: Application to Protein Oxidations that affect Human Health and Wellbeing
Lead Research Organisation:
Imperial College London
Department Name: Chemistry
Abstract
New and novel measurement tools and technologies enable modern biological and biomedical research to continue to move forward at a rapid rate. Detecting disease and evaluating the effects of drugs all rely on measurement technologies, and the limitations in our ability to measure things rapidly, accurately and cheaply is often the bottleneck in many research areas. Similarly, advances in industrial processes and clinical methods are often dependent on matching advances in measurement technology. In this programme two leading research centres from Glasgow University and Imperial College London join forces to develop new techniques and tools in order to improve existing approaches to detect and quantify the effects of protein oxidative damage in biological samples. Protein oxidative damage is one of the components of ageing and can cause a range of serious medical problems. As part of this project we will improve the ways in which biological samples are delivered into a range of analytical platforms such as mass spectroscopy and protein microarrays by employing novel innovative approaches such as acoustic sound waves, emulsion droplets and microfludics. Chemical tools and probes that seek out and detect damaged proteins will be constructed and subsequently incorporated into the technologies mentioned above. These technologies will be used to study the dynamics of protein damage in space and time. Another part of the programme develops high throughput analysis tools of various forms all of which use minaturisation methods to allow for the analysis of as many proteins as possible, as rapidly as possible and with as much detail as possible. It is anticipated that this multidisciplinary programme will create the necessary analytical platforms to overcome a technological gap that is holding back biological and medical discoveries in this research area that is vital for fostering our understanding of human health and ageing. It also has the potential to facilitate medical interventions to alleviate the symptoms of ageing and age-related illness. Given the importance of these issues for the general population, not just of the UK but worldwide, the impact of this programme will be significant and sustained for several years in terms of academic output as well as industrial wealth generation. In particular, novel drug targets and indeed drugs, as well as improved diagnostic techniques to monitor and assess human health and ageing are possible outcomes in the near future. Given the importance of oxidative damage in crop production it is likely that our programme will also produce tools that benefit the agrochemical industry, making this proposal a truly discipline crossing venture with respect to the sciences involved and the impact generated.
Planned Impact
This project will be of benefit to both the academic and industrial communities and is aligned with three of the EPSRC's strategic areas (Nanoscience through engineering to application, Ageing-Lifelong Health and Wellbeing, and Towards Next Generation Healthcare). The programme which capitalises upon seven-state-of the-art technologies developed within the ICB DTC and C&PT DTCs will realise a number of key advances with broad-reaching application. It is directly focused on the development of tools that will enable drug discovery and disease proteomics, with improved healthcare as the long-term goal. The advances that will benefit both sectors include technical improvements in mass spectrometry, multidimensional spectroscopy, chemical probes, protein arrays, microfluidics and proteomic activity profiling. In addition, the tools and techniques under development here have the potential to provide enhanced capabilities for end-users in basic biology, biomarker discovery, diagnostics and drug discovery. These applications can be directly exploited by the pharmaceutical industry, medical sector, biotechnology SMEs and instrumentation manufacturers. The project builds on our existing doctoral training programmes and will provide training of a cohort of PDRAs and students in the important area of post-genomic technology and novel analytical tools. There remains an unmet need for highly capable multidisciplinary scientists with genuine expertise across more than one discipline. Our programme will produce future designers and developers of new measurement techniques and strategies via a cohort who understand both a range of technologies and the details of the biological and biomedical challenges to be addressed. The transferable skills we will provide will include courses on systems integration, entrepreneurship and exploitation, allowing them to translate and exploit their research both during the project and their future careers. The focus of the programme is concerned with providing tools for the better study of and intervention into oxidative damage of proteins, which is one of the molecular components of ageing. There are obvious benefits to the general population of research that has the potential to impact on the adverse effects of ageing and improve quality of life. UK industry will benefit from the scientific and technical innovations we will produce, with a 5-10 year timeframe for commercial realisation. The diagnostics tools that we will develop will not only be able to detect the changes associated with disease or ageing but also, and most importantly, to monitor their progress during the drug development process and subsequent therapy (10 yrs). The economic imperative is for medicine to be more preventative; these tools will play a vital role by facilitating the identification of illness in its early stages, allowing for early intervention to reduce permanent damage (10 yrs) and alleviating associated healthcare costs. In the context of drug discovery, the ability to understand and study the enzyme active sites and peripheral damage to easily oxidised signalling systems paves the way for novel intervention strategies either to mitigate aging effects or to target these proteins as part of an intervention for other diseases. Our technologies have the potential both to identify and to validate targets and biomarkers, as well as testing for side effects of drug candidates (5-10 yrs). Individual elements such as the MAC chips and 2DIR will be exploitable for clinical diagnostics and drug discovery on shorter time scales (ca. 3-5 yrs) and the proteomic technologies we will develop can be applied to any disease, including cancer, and cardiovascular diseases. The result will be improved healthcare as well as enhanced economic competitiveness of the United Kingdom by generating wealth and potential future employment.
Organisations
- Imperial College London (Lead Research Organisation)
- Mendeley Ltd (Collaboration)
- Cyclofluidic (Collaboration)
- DIAMOND LIGHT SOURCE (Collaboration)
- IMPERIAL COLLEGE LONDON (Collaboration)
- UNIVERSITY OF EXETER (Collaboration)
- Technical University of Denmark (Collaboration)
- UNIVERSITY OF GLASGOW (Collaboration)
- ASTON UNIVERSITY (Collaboration)
- LIVERPOOL JOHN MOORES UNIVERSITY (Collaboration)
- University of Valencia (Collaboration)
- UNIVERSITY OF LEEDS (Collaboration)
- UNIVERSITY OF LIVERPOOL (Collaboration)
- Aalto University (Collaboration)
- KING'S COLLEGE LONDON (Collaboration)
Publications
Aldini G
(2015)
Protein lipoxidation: Detection strategies and challenges
in Redox Biology
Burgin E
(2014)
Absolute quantification of protein copy number using a single-molecule-sensitive microarray.
in The Analyst
Casey D
(2016)
Essentials of Single-Cell Analysis
Casey D
(2014)
Amphiphilic drug interactions with model cellular membranes are influenced by lipid chain-melting temperature.
in Journal of the Royal Society, Interface
Chanasakulniyom M
(2015)
Cell proliferation and migration inside single cell arrays.
in Lab on a chip
Cilibrizzi A
(2017)
Small-molecule optical probes for cell imaging of protein sulfenylation and their application to monitor cisplatin induced protein oxidation
in Sensors and Actuators B: Chemical
Cilibrizzi A
(2017)
A tri-functional vanadium(iv) complex to detect cysteine oxidation.
in Dalton transactions (Cambridge, England : 2003)
Collins J
(2016)
Vanadyl complexes with dansyl-labelled di-picolinic acid ligands: synthesis, phosphatase inhibition activity and cellular uptake studies.
in Dalton transactions (Cambridge, England : 2003)
Dent MR
(2015)
Imaging phase separation in model lipid membranes through the use of BODIPY based molecular rotors.
in Physical chemistry chemical physics : PCCP
Guo R
(2012)
Geometry determination of complexes in a molecular liquid mixture using electron-vibration-vibration two-dimensional infrared spectroscopy with a vibrational transition density cube method.
in Physical chemistry chemical physics : PCCP
Houée-Lévin C
(2015)
Exploring oxidative modifications of tyrosine: an update on mechanisms of formation, advances in analysis and biological consequences.
in Free radical research
Lanigan PM
(2012)
Dynamical hologram generation for high speed optical trapping of smart droplet microtools.
in Biomedical optics express
Magness A
(2017)
Multiplexed single cell protein expression analysis in solid tumours using a miniaturised microfluidic assay
in Convergent Science Physical Oncology
Paterson DJ
(2014)
Integrating microfluidic generation, handling and analysis of biomimetic giant unilamellar vesicles.
in Lab on a chip
Reboud J
(2013)
Acoustically controlled enhancement of molecular sensing to assess oxidative stress in cells.
in Chemical communications (Cambridge, England)
Reboud J
(2012)
Shaping acoustic fields as a toolset for microfluidic manipulations in diagnostic technologies.
in Proceedings of the National Academy of Sciences of the United States of America
Reboud J
(2012)
Nebulisation on a disposable array structured with phononic lattices.
in Lab on a chip
Reboud J
(2019)
Paper-based microfluidics for DNA diagnostics of malaria in low resource underserved rural communities.
in Proceedings of the National Academy of Sciences of the United States of America
Rezende Valim L
(2014)
Identification and relative quantification of tyrosine nitration in a model peptide using two-dimensional infrared spectroscopy.
in The journal of physical chemistry. B
Salehi-Reyhani A
(2015)
Chemical-free lysis and fractionation of cells by use of surface acoustic waves for sensitive protein assays.
in Analytical chemistry
Salehi-Reyhani A
(2013)
Scaling advantages and constraints in miniaturized capture assays for single cell protein analysis.
in Lab on a chip
Salehi-Reyhani A
(2014)
Addressable droplet microarrays for single cell protein analysis.
in The Analyst
Sandison ME
(2014)
Magnetite-doped polydimethylsiloxane (PDMS) for phosphopeptide enrichment.
in The Analyst
Schrems A
(2014)
The grab-and-drop protocol: a novel strategy for membrane protein isolation and reconstitution from single cells.
in The Analyst
Sowley H
(2019)
Detection of Drug Binding to a Target Protein Using EVV 2DIR Spectroscopy.
in The journal of physical chemistry. B
Spickett CM
(2013)
Use of narrow mass-window, high-resolution extracted product ion chromatograms for the sensitive and selective identification of protein modifications.
in Analytical chemistry
Tassieri M
(2015)
Microrheology with optical tweezers: measuring the relative viscosity of solutions 'at a glance'.
in Scientific reports
Tiller B
(2017)
Frequency dependence of microflows upon acoustic interactions with fluids
in Physics of Fluids
Tveen-Jensen K
(2013)
Reporter ion-based mass spectrometry approaches for the detection of non-enzymatic protein modifications in biological samples.
in Journal of proteomics
Tveen-Jensen K
(2015)
Interfacing low-energy SAW nebulization with Liquid Chromatography-Mass Spectrometry for the analysis of biological samples.
in Scientific reports
Vergelli C
(2016)
2-Arylacetamido-4-phenylamino-5-substituted pyridazinones as formyl peptide receptors agonists.
in Bioorganic & medicinal chemistry
Verrastro I
(2016)
Reversible oxidation of phosphatase and tensin homolog (PTEN) alters its interactions with signaling and regulatory proteins.
in Free radical biology & medicine
Verrastro I
(2015)
Mass spectrometry-based methods for identifying oxidized proteins in disease: advances and challenges.
in Biomolecules
Villangca M
(2015)
Optically-controlled platforms for transfection and single- and sub-cellular surgery.
in Biophysical reviews
Willison K
(2013)
Quantitative single cell and single molecule proteomics for clinical studies
in Current Opinion in Biotechnology
Witte C
(2014)
Microfluidic resonant cavities enable acoustophoresis on a disposable superstrate.
in Lab on a chip
Witte C
(2014)
Spatially selecting a single cell for lysis using light-induced electric fields.
in Small (Weinheim an der Bergstrasse, Germany)
Xu G
(2016)
Paper-Origami-Based Multiplexed Malaria Diagnostics from Whole Blood
in Angewandte Chemie
Xu G
(2016)
Paper-Origami-Based Multiplexed Malaria Diagnostics from Whole Blood.
in Angewandte Chemie (International ed. in English)
Xu G
(2019)
Branched hybridization chain reaction-using highly dimensional DNA nanostructures for label-free, reagent-less, multiplexed molecular diagnostics.
in Microsystems & nanoengineering
| Title | Large Cell picture from Royal Society Summer Exhibition |
| Description | Large backlit image of a fluorescent cell used as the back panel of our stand at the RSSE. |
| Type Of Art | Artwork |
| Year Produced | 2015 |
| Impact | Since the end of the exhibition the panel has been moved to Imperial college where it will become a permanent feature in the Chemistry department. |
| Title | MAC chip animatiosn |
| Description | A series of animations showing the use of the MAC chip platform step by step. These animations follow one single cell taken from the blood of a cancer patient and follow it through optical trapping, optical lysis and finally single molecules detection of the proteins within. Each animation is overlayed with real video footage from a device for comparison. |
| Type Of Art | Film/Video/Animation |
| Year Produced | 2013 |
| Impact | These animations have been used by multiple members of the group for presentation purposes and have received a lot of interest. For example the animations were used by a PhD student in his end of PhD postgraduate symposium which lead to him receiving the second place prize for final year PhD talk. |
| Title | Model Mass Spectrometer |
| Description | A model showing how a magnetic field can be used to sort different sized objects. Metal beads of different sizes are launched down a ramp and pass near a strong magnet. The magnet changes the path of these beads and directs them to one of many destinations. In effect, it demonstrates the main principle behind mass spectroscopy and is a great educational tool. |
| Type Of Art | Artistic/Creative Exhibition |
| Year Produced | 2014 |
| Impact | Used in a number of public engagement events including the 2014 World science fiction convention in London. |
| Title | PROXO Workstream Videos |
| Description | These videos show the different workstreams of our project and how they intercalate. They are available on our webpage. |
| Type Of Art | Film/Video/Animation |
| Year Produced | 2014 |
| Impact | These videos are a really easy way for new collaborators to familiarise themselves with our project and research. |
| Title | PROXO video |
| Description | The PROXO video describes the main focus of the research and is available on the proxomics webpage. |
| Type Of Art | Film/Video/Animation |
| Year Produced | 2014 |
| Impact | The video is a really easy means to tell people what our project is about and we have had lots of positive feedback from collaborators and conference attendees. |
| URL | http://www.proxomics.ac.uk/ |
| Title | Proxomics.ac.uk |
| Description | Our Proxomics webpage is primarily a tool to engage the public and collaborators in our research and disseminate our progress and our activities. It features many videos and graphical illustrations on how we have arranged our multidisciplinary work streams to work together. |
| Type Of Art | Artefact (including digital) |
| Year Produced | 2012 |
| Impact | We've obtained lots of collaborative interest through the information advertised on our website. |
| URL | http://www.proxomics.ac.uk/ |
| Title | The Cell Panel |
| Description | The Cell panel was created for the RSSSE exhibition. It is an interactive cell that shows how protein interactions can affect cell signalling and how damage to signalling proteins can result in cancer. |
| Type Of Art | Artwork |
| Year Produced | 2015 |
| Impact | The cell panel was well visited at The RSSSE and drew a lots of interest from the younger visitors. |
| Description | We have developed a suite of tools and technologies able to study the effect of protein oxidation and oxidative stress on human biology with particular reference to the development of a range of diseases. These are now being used for biological and biomedical studies in follow-up projects. |
| Exploitation Route | Many of the methods developed are in use for both academic projects but also for collaborative work with industry and clinical researchers. |
| Sectors | Healthcare,Manufacturing, including Industrial Biotechology,Pharmaceuticals and Medical Biotechnology |
| Description | The innovative tools and techniques of this project have opened up industry collaborations and are continuously sought after when presented at in-the-field as well as cross-disciplinary conferences. Some of the methods are being used in follow up applications with both industry and clinical researchers. We now have evidence that we are able to detect the effects of oxidative stress causing senescence in single live cells from sputum from patients with COPD. |
| Sector | Healthcare,Pharmaceuticals and Medical Biotechnology |
| Impact Types | Economic |
| Description | CASE Award |
| Amount | £92,000 (GBP) |
| Funding ID | BB/J012939/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | |
| Description | COST Action Award |
| Amount | £1,100 (GBP) |
| Organisation | European Cooperation in Science and Technology (COST) |
| Sector | Public |
| Country | Belgium |
| Start | |
| Description | DTA Awards, PhD bursaries |
| Amount | £240,000 (GBP) |
| Funding ID | EP/J500239/1 |
| Organisation | Engineering and Physical Sciences Research Council (EPSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 10/2011 |
| End | 09/2015 |
| Description | EPSRC Fellowship |
| Amount | £1,538,918 (GBP) |
| Funding ID | EP/K027611/1 |
| Organisation | Engineering and Physical Sciences Research Council (EPSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | |
| Description | Farmed Animal Disease and Health (FADH) [2013] |
| Amount | £437,440 (GBP) |
| Funding ID | BB/L00464X/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | |
| Description | Impact Acceleration Award |
| Amount | £75,066 (GBP) |
| Funding ID | EP/K503733/1 |
| Organisation | Engineering and Physical Sciences Research Council (EPSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | |
| Description | Institutional Strategic Support Fund: Network of Excellence Scheme |
| Amount | £100,000 (GBP) |
| Organisation | Wellcome Trust |
| Department | Wellcome Trust Institutional Strategic Support Fund |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | |
| Description | Knowledge Transfer Secondments; Impact Acceleration Account |
| Amount | £46,344 (GBP) |
| Funding ID | EP/K503733/1 |
| Organisation | Engineering and Physical Sciences Research Council (EPSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | |
| Description | Lowe Syndrome Trust |
| Amount | £330,000 (GBP) |
| Organisation | Lowe Syndrome Trust |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | |
| Description | SSSC |
| Amount | £40,000 (GBP) |
| Organisation | Government of Scotland |
| Department | Scottish Funding Council |
| Sector | Public |
| Country | United Kingdom |
| Start | |
| Description | Social entrepreneurship funding |
| Amount | £500 (GBP) |
| Organisation | UnLtd |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | |
| Description | Technology Strategy Board (TSB) [2011-2014] |
| Amount | £243,768 (GBP) |
| Funding ID | BB/L011336/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | |
| Description | Technology Strategy Board (TSB) [2011-2014] |
| Amount | £243,768 (GBP) |
| Funding ID | BB/L011336/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | |
| Title | 2DIR spectrometer |
| Description | Construction of 2DIR Spectrometer and applying it to the detection of oxidized protein side chains. |
| Type Of Material | Technology assay or reagent |
| Provided To Others? | No |
| Impact | 2DIR has been able to successfully detect oxidised forms of proteins and this has led to publication. |
| Title | Chip surface passivation protocols |
| Description | To limit the amount of non-specific binding to surface to maintain sensitivity silane, PEG and 3D polymer based surface chemistry was investigated. It was determined that silane based chemistry is appropriate for DNA assays whereas PEG based chemistries are better suited for protein assays. |
| Type Of Material | Improvements to research infrastructure |
| Provided To Others? | No |
| Impact | These passivated slides are required for use with the MAC chip platform a vital part of the Proxomics project. |
| Title | Humidity cell for 2DIR experiments |
| Description | A humidity cell was made from a cheap optical mount, a CaF2 window, a glass coverslip, PTFE spacers and a rubber o-ring. The biological sample of interest is placed on the glass slide along with several drops of saturated salt solution, which enable a constant humidity to be maintained inside the cell for at least a month. This ensures that the water content of the biological sample of interest remains constant throughout a series of 2DIR experiments. |
| Type Of Material | Improvements to research infrastructure |
| Provided To Others? | No |
| Impact | This humidity cell is much smaller than commercial humidity cells and is easier to mount on our 2DIR sample stage, allowing for a quicker and more reproducible experimental set-up. It is also much cheaper than commercial cells, which means that we have made 5 of these humidity cells containing different biological samples for the same price as just 1 commercial cell. This leads to higher throughput in our 2DIR experiments. |
| Title | Microfluidic Antibody Capture Chips |
| Description | Designed and built microfluidic antibody capture chips, an all optical platform for the quantification of protein in single cells. Cells are loaded into these devices, transported to analysis chambers using an optical trap and then ruptured with the use of a high powered optical lysis laser. The intracellular contents are then quantified by the use of a miniaturized ELISA format. Proteins bind to antibodies on the surface of the device and a secondary fluorescent antibody, present in solution completes the sandwich system. Total Internal Reflection Fluorescence (TIRF) microscopy is then used to detect and count the amount of protein that has bound to the antibody spot. |
| Type Of Material | Technology assay or reagent |
| Provided To Others? | No |
| Impact | The use of MAC chips dominates an entire work stream in the Proxomics project and has resulted in a number of publications. |
| Title | Microfluidic antibody capture (MAC) chip design |
| Description | A microfluidic antibody capture chip was designed to allow to isolation of single cells from a population, their subsequent lysis and binding of a protein or proteins of interest to an antibody patch. Separate designs for the isolation of single, few (1-5) and many (10-100) cells from flow were also made. |
| Type Of Material | Technology assay or reagent |
| Provided To Others? | No |
| Impact | MAC chips dominate an entire workstream of the proxomics project and have resulted in a number of publications. |
| Title | Microfluidic chips design for handling Smart Droplet Microtools and cells |
| Description | Microfluidic chips design for handling Smart Droplet Microtools and cells |
| Type Of Material | Technology assay or reagent |
| Provided To Others? | No |
| Impact | Smart Droplet microtools and the associated platform have led to the publication of several papers. |
| Title | Opto/Thermoporation of single cells |
| Description | The discovery that poly-silicon can be used to cause cell membrane phase changes when irradiated with 1070nm laser light the technique. |
| Type Of Material | Improvements to research infrastructure |
| Year Produced | 2015 |
| Provided To Others? | Yes |
| Impact | Has the ability to revolutionise cell transfection. Can selectively permeable single cells in a microfluidic environment which can then take up a variety of otherwise cell impermeable reagents. |
| Title | Plasmids tagging VHR with HA and Flag |
| Description | Plasmids pcDNA-3.1-Flag-VHR and pcDNA-3.1-HA-VHR, to express N-terminal tagged VHR. |
| Type Of Material | Biological samples |
| Provided To Others? | No |
| Impact | Enabled tagged VHR to be expressed in cell lines and detected using different antibodies |
| Title | Protocols for cell handling onto chips |
| Description | Cells were detached from flasks using gentle tryptic agents and filtered using a nylon mesh. Cells are suspended in media using a CO2 independent buffer. Cells are delivered via capillary tubing. |
| Type Of Material | Improvements to research infrastructure |
| Provided To Others? | No |
| Impact | Cells are used in microfluidic devices for both the MAC chip and SDM platforms both of which have resulted in publications |
| Title | SAW Cell Lysis |
| Description | A chemical free lysis method for use in lab on a chip device. Surface acoustic waves use to mechanically lyse a cell sample for downstream analysis. |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2014 |
| Provided To Others? | Yes |
| Impact | Chemcial free lysis based on acoustic force can be implemented in point-of-care devices and easily coupled to downstream diagnostic applications |
| Title | SAW tunnels to spatially position a SAW beam at a microfluidic chamber/channel |
| Description | A problem with SAW streaming in PDMS mirochannels is the damping effect of the PDMS wall. I used a dry channel to allow the SAW to effectively tunnel through the PDMS wall. This concept also allows for the precise positioning of the Saw in the chamber |
| Type Of Material | Improvements to research infrastructure |
| Provided To Others? | No |
| Impact | A potential useful tool for designing and realizing SAW acoustophoresis and streaming devices |
| Title | SAWN LC/MS |
| Description | Combination of the SAW nebulsation with mass spectroscopy for a more gentle sample prep |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2012 |
| Provided To Others? | Yes |
| Impact | The technique may potential be used in standard proteomic mass spectroscopy work flows where electrospray is deemed unsuitable |
| Title | Single Cell Antibody printing |
| Description | All antibodies were printed by an OmniGrid Micro microarrayer (Digilab, UK) using 946MP2 pins (ArrayIt, USA). Spotting solution was optimised for DNA, antibody, protein or cell-lysate printing. |
| Type Of Material | Improvements to research infrastructure |
| Provided To Others? | No |
| Impact | Antibody printing is a vital step in the MAC chip platform. This platform is a vital part of the proxomics project and has resulted in a number of publications. |
| Title | Single Cell Trapping array |
| Description | A microfluidic device optimised for capturing cells in an array for subsequent single cell analysis |
| Type Of Material | Technology assay or reagent |
| Provided To Others? | No |
| Impact | Will be of interest in the field of microfluidics and single cell analysis. Poster describing the method was shortlisted for poster prize at Microfluidics 2014 conference |
| Title | Single Cell lysis protocols |
| Description | A technique using a short pulsed laser is employed to lyse single cells. Through a series of physical processes a cavitation bubble expands from the focal point of the laser. The shearing forces experienced by the cell placed approximately 10_m away disrupts the cell and liberates intracellular constituents for analysis. It has been demonstrated that the cell may be fully (all membranes ruptured including nuclear membrane) or partially (only plasma membrane ruptured preserving the nucleus intact) lysed. The optical lysis process is extremely fast whereby the cell is disrupted within a few microseconds. |
| Type Of Material | Improvements to research infrastructure |
| Provided To Others? | No |
| Impact | Optical lysis is a vital part of the Microfluidic antibody capture platform which has resulted in a number of publications. |
| Title | Sorting of Low Abundance Cells from Small Clinical Samples for downstream single cell analysis |
| Description | We have shown that it is possible to use the laser trap in combination with surface markers to select cells of interest from a heterogeneous mix. The cells are selected by type and then moved into the analysis section of the MAC chip for assessment of their signalling status as read out by protein copy number and PTM condition. |
| Type Of Material | Improvements to research infrastructure |
| Year Produced | 2016 |
| Provided To Others? | Yes |
| Impact | We are now in a position to demonstrate the utility of this approach in clinical contexts. A pilot project with ICR has been completed and a paper submitted and is under review. Two other projects are underway, one with NHLI/Brompton Hospital on COPD and another with Kings College on the activation status of immune cells in human tumours. |
| Title | Sorting of different sized particles using a combination of SAW streaming and acoustophoresis |
| Description | Looked at using tunnels to create a vortex that can trap and concentration small particles (micrometer diam). Bigger particles are pushed away by acoustic radiation force (10 micro meters) |
| Type Of Material | Improvements to research infrastructure |
| Provided To Others? | No |
| Impact | Could be an interesting way of concentrating and refining very small cells, for example verticals and exosomes in a blood sample. What would be particularly novel would be the use of streaming vortices to trap and concentrate very small particles /cells/vesicles against a sheath flow |
| Title | TIRF-FLIM system |
| Description | Combined total internal reflection fluorescence lifetime imaging system for improved resolution to conventional fluorescence lifetime imaging techniques |
| Type Of Material | Improvements to research infrastructure |
| Provided To Others? | No |
| Impact | Enhanced the capabilities of standard fluorescence lifetime imaging through improved axial resolution. Technology is of particular use for investigations of dynamics in biological membranes. |
| Title | A simulation of the SAW induced streaming in a microfluidic chamber/channel |
| Description | Created a novel simulation for describing the SAW streaming inside microfluidic volumes. This involved analytical and FEM parts |
| Type Of Material | Computer model/algorithm |
| Provided To Others? | No |
| Impact | This should be a useful too for researchers designing SAW devices with the intention of creating streaming flows in mirofluidic chambers. With this simulation it was possible to create scaling laws between frequency and the chamber length/height which can guide the optimum selection of SAW frequency for a given microfluidic set-up |
| Title | Acoustic suppression of the coffee-ring effect. |
| Description | We study the influence of acoustic fields on the evaporative self-assembly of solute particles suspended inside sessile droplets of complex fluids. The self-assembly process often results in an undesirable ring-like heterogeneous residue, a phenomenon known as the coffee-ring effect. Here we show that this ring-like self-assembly can be controlled acoustically to form homogeneous disc-like or concentrated spot-like residues. The principle of our method lies in the formation of dynamic patterns of particles in acoustically excited droplets, which inhibits the evaporation-driven convective transport of particles towards the contact line. We elucidate the mechanisms of this pattern formation and also obtain conditions for the suppression of the coffee-ring effect. Our results provide a more general solution to suppress the coffee-ring effect without any physiochemical modification of the fluids, the particles or the surface, thus potentially useful in a broad range of industrial and analytical applications that require homogenous solute depositions. |
| Type Of Material | Database/Collection of data |
| Year Produced | 2015 |
| Provided To Others? | Yes |
| Title | Approximate Bayesian Computation (ABC) |
| Description | Parameter inference algorithm for stochastic simulations |
| Type Of Material | Computer model/algorithm |
| Provided To Others? | No |
| Impact | Has been used to develop a model of p53 expression and compare it with experimental data. |
| Title | Capture Assay Model |
| Description | A software that models the scaling advantages and constraints in miniaturized capture assays for single cell proteomics |
| Type Of Material | Computer model/algorithm |
| Provided To Others? | No |
| Impact | Used in group to predict assay behaviour and inform assay validation |
| Title | Frequency dependence of microflows upon acoustic interactions with fluids |
| Description | This is the raw data for the results presented in the manuscript. It includes images, simulation models and csv files. |
| Type Of Material | Database/Collection of data |
| Year Produced | 2017 |
| Provided To Others? | Yes |
| Title | GDSC PALM Counting Algorithm |
| Description | Single molecule localisation counting algorithm. |
| Type Of Material | Computer model/algorithm |
| Provided To Others? | No |
| Impact | Counting algorithm essential to the MAC chip platform. Replaces older counting algorithm and has provided better results. |
| Title | Lipid topology and linear cationic antimicrobial peptides: a novel mechanistic model |
| Description | The dataset is a dedicated LabVIEW virtual instrument, for the analysis of dye-efflux dynamics. The instrument is capable of automatically extracting the apparent permeability from the leakage of encapsulated fluorescent markers, from within artificial cell systems. |
| Type Of Material | Database/Collection of data |
| Year Produced | 2017 |
| Provided To Others? | Yes |
| Title | Micro PIV and vibrometer measurements of SAW streaming in microfluidic chamber |
| Description | A combination of micro PIV and vibrometer measurements that were collected to validate the simulation (see above) for a range of different SAW frequencies and powers |
| Type Of Material | Database/Collection of data |
| Provided To Others? | No |
| Impact | Helps to enhance knowledge/models of SAW streaming in microfluidic chambers/channels |
| Title | Origami-based Multiplexed Infectious Disease Diagnostics from Whole Blood |
| Description | |
| Type Of Material | Database/Collection of data |
| Year Produced | 2016 |
| Provided To Others? | Yes |
| Title | Paper-based Microfluidics for DNA Diagnostics of Malaria in Low Resource Under Served Rural Communities |
| Description | This is the raw data for the results presented in the linked manuscript. |
| Type Of Material | Database/Collection of data |
| Year Produced | 2019 |
| Provided To Others? | Yes |
| Description | Ajay Mahalka |
| Organisation | Aalto University |
| Country | Finland |
| Sector | Academic/University |
| PI Contribution | Mass spectrometry analysis of Phosphalipase A2 modified with PoxnoPC |
| Collaborator Contribution | n/a |
| Impact | n/a |
| Start Year | 2012 |
| Description | Ben Tiller and Michael Dent |
| Organisation | Imperial College London |
| Department | Department of Chemistry |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Use of fluorescence microscopy/spectroscopy and operational expertise. |
| Collaborator Contribution | Synthesised molecular rotor molecules to be investigated. |
| Impact | Led to a joint publication between Glasgow and Imperial with the full details in the publications section of ResearchFish DOI:10.1039/C5CP01937K |
| Start Year | 2014 |
| Description | Closing The Loop on Drug Discovery With Adam Nelson |
| Organisation | University of Leeds |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | At the moment it is purely conceptual, but we have explored how EVV 2DIR spectroscopy could be used as a one stop biophysical method for rapidly determining both binding affinity and geometry of binding for a drug interacting with a protein target. We have also thought through how this will be formatted in high throughput and made a project application to the Rosalind Franklin Institute to turn the idea into reality. |
| Collaborator Contribution | At the moment it is purely conceptual, but we have explored how EVV 2DIR spectroscopy could be used as a one stop biophysical method for rapidly determining both binding affinity and geometry of binding for a drug interacting with a protein target. We have also thought through how this will be formatted in high throughput and made a project application to the Rosalind Franklin Institute to turn the idea into reality. |
| Impact | Project application to the Rosalind Franklin Institute |
| Start Year | 2017 |
| Description | Dr Corinne Spickett |
| Organisation | Aston University |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Dr Corinne Spickett is a collaborator and co-I on the grant 'Next Generation Analytical Tools: Application to Protein Oxidations that affect Human Health and Wellbeing'. |
| Start Year | 2011 |
| Description | Dr Ekaterina McKenna |
| Organisation | University of Glasgow |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | n/a |
| Collaborator Contribution | Developing new array technologies and applying them to investigate interaction of Phosphatases |
| Impact | n/a |
| Start Year | 2012 |
| Description | Dr Elizabeth Farrant |
| Organisation | Cyclofluidic |
| Country | United Kingdom |
| Sector | Private |
| PI Contribution | Knowledge transfer of single-cell technology. Design of custum built cell assay for dynamic single-cell resolution drug discovery. |
| Collaborator Contribution | Provided resources in the form of reagents and drug compounds. Transfer of expertise in autonomous drug discovery. |
| Impact | This collaborative research has resulted in further funding by a Knowledge Transfer Secondment of the EPSRC. The work is truly multidisciplinary where Cyclofluidic are a pharmaceutical company collaborating with our chemical biology and physical research team that is also engaged in engineering a new platform to integrate new cell assay technologies. |
| Start Year | 2012 |
| Description | Dr Euan Stronach |
| Organisation | Imperial College London |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | MAC chip analysis of ovarian cancer cells, specifically the analysis of AKT and p53 protein copy number in single cells. Immunohistochemistry of ovarian cancer cells from Ascites fluid with several cancer markers. |
| Collaborator Contribution | Provision of ovarian cancer cells from Ascites fluid. Provision of circulating cancer cells from patients with ovarian cancer. |
| Impact | Further funding has been obtained for this collaboration in the form of a Pathway to Impact grant from the EPSRC. |
| Start Year | 2013 |
| Description | Dr Jesper Glückstad |
| Organisation | Technical University of Denmark |
| Country | Denmark |
| Sector | Academic/University |
| PI Contribution | Erasmus-funded visit (proposal in progress); joint biophotonics research |
| Collaborator Contribution | Optical probe development, instrument access, joint experiments |
| Impact | Joint paper, new lines of enquiry |
| Start Year | 2014 |
| Description | Dr Paul Donaldson |
| Organisation | Diamond Light Source |
| Country | United Kingdom |
| Sector | Private |
| PI Contribution | Imaging of nitrated human tissue comparing 2DIR and IR imaging. |
| Collaborator Contribution | Provision of human tissue. |
| Impact | n/a |
| Start Year | 2014 |
| Description | Dr Stuart Bennett |
| Organisation | Aston University |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | MS identification of membrane proteins from Red blood cells. |
| Collaborator Contribution | n/a |
| Impact | n/a |
| Start Year | 2013 |
| Description | Duncan Casey and Jayne Dooley at LMJU |
| Organisation | Liverpool John Moores University |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Joint Paper |
| Collaborator Contribution | Joint Paper |
| Impact | Book Chapter which is in Publications section of ResearchFish |
| Start Year | 2014 |
| Description | Jose Luis Garcia Gimerez |
| Organisation | University of Valencia |
| Country | Spain |
| Sector | Academic/University |
| PI Contribution | n/a |
| Collaborator Contribution | Analysing oxidative modifications to Histone samples from 2 cell lines, one derived from young patients and one from old patients. |
| Impact | n/a |
| Start Year | 2012 |
| Description | Mendeley |
| Organisation | Mendeley Ltd |
| Country | United Kingdom |
| Sector | Private |
| PI Contribution | Substantial consultation and development work undertaken. Work has included direct product development, collating user feedback, assistance with their institutional edition and liaison with information management staff. Helping specify and adapt their institutional edition package with Swets and Library staff |
| Collaborator Contribution | Free access to Mendeley Ltd, a software company selling collaborative reference management, software ideal for large, multidisciplinary groups. |
| Impact | This collaboration has given the research group a great opportunity to easily store and share publications related to our collaborative research. Papers and relevant reviews are easily shared and discussed online. |
| Start Year | 2009 |
| Description | Prof Chris Braddock |
| Organisation | Imperial College London |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Identify potential activators or inhibitors of SHP2 phosphatase by screening a library of compounds synthesized in Chris Braddock's lab. |
| Collaborator Contribution | Provided a library of compounds synthesized in Chris Braddock's lab. |
| Impact | n/a |
| Start Year | 2013 |
| Description | Prof Malcom Jackson |
| Organisation | University of Liverpool |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Mass spec analysis of young and aged mice muscle cells. |
| Collaborator Contribution | Provided young and aged mice muscle for mass spec analysis. |
| Impact | n/a |
| Start Year | 2013 |
| Description | Prof Michael Stumpf |
| Organisation | Imperial College London |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Single cell distributions showing the heterogeneous action of the anti-cancer agent Actinomycin D. |
| Collaborator Contribution | Stochastic Simulations were performed using the provided data. Approximate Bayesian Computation was used to infer parameters which would make the distributions obtained from MAC chip data. |
| Impact | n/a |
| Start Year | 2014 |
| Description | Prof Michael Tisdale |
| Organisation | Aston University |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Identifying a human Bif receptor using mass spectrometry |
| Collaborator Contribution | n/a |
| Impact | n/a |
| Start Year | 2013 |
| Description | Prof Paul Winyard |
| Organisation | University of Exeter |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | MS analysis of nitrated BSA and HAS |
| Collaborator Contribution | n/a |
| Impact | n/a |
| Start Year | 2013 |
| Description | Prof Sunil Shaunak |
| Organisation | Imperial College London |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | FTIR studies of dendrimer-based drugs. |
| Collaborator Contribution | Provision of dendrimer-based drugs. |
| Impact | n/a |
| Start Year | 2013 |
| Description | Prof Tony Ng |
| Organisation | King's College London |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | n/a |
| Collaborator Contribution | Investigating protein-protein interactions involving the SHP2 phosphatase under oxidative stress conditions. |
| Impact | n/a |
| Start Year | 2013 |
| Description | Professor Andy Pitt |
| Organisation | Aston University |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Professor Andy Pitt is a collaborator and co-I on the grant 'Next Generation Analytical Tools: Application to Protein Oxidations that affect Human Health and Wellbeing'. |
| Start Year | 2011 |
| Description | Professor Jon Cooper |
| Organisation | University of Glasgow |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Professor Jon Cooper is a collaborator and co-I on the grant 'Next Generation Analytical Tools: Application to Protein Oxidations that affect Human Health and Wellbeing'. |
| Start Year | 2011 |
| Description | Professor Michael Stumpf |
| Organisation | Imperial College London |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Professor Michael Stumpf is a collaborator on the grant 'Cellular Information Processing and Decision Making from Noise to Robust Phenotypes'. |
| Start Year | 2011 |
| Description | Sir Steve Bloom |
| Organisation | Imperial College London |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | 2DIR measurements on drug binding. |
| Collaborator Contribution | Providing drugs for 2DIR measurements. |
| Impact | n/a |
| Start Year | 2013 |
| Title | DEVICE AND METHOD FOR CAUSING AGGREGATION OF BLOOD CELLS AND METHOD OF TREATING BLEEDING FROM AN INJURY |
| Description | A device is disclosed for causing aggregation of blood cells to affect one or more fluid properties of blood. The device comprises: a surface acoustic wave (SAW) propagation surface configured to be brought into acoustic contact with blood; and a transducer arranged to provide SAWs on the SAW propagation surface that when coupled with blood in acoustic contact with the SAW propagation surface cause aggregation of blood cells in the blood to affect one or more fluid properties of the blood. Also disclosed are a method of causing aggregation of blood cells to affect one or more fluid properties of blood and a method of treating bleeding from an injury. |
| IP Reference | WO2015033139 |
| Protection | Patent granted |
| Year Protection Granted | 2015 |
| Licensed | No |
| Impact | background for spin out |
| Title | Fluidics apparatus for surface acoustic wave manipulation of fluid samples, use of fluidics apparatus and process for the manufacture of fluidics apparatus |
| Description | A fluidics apparatus for manipulation of at least one fluid sample is disclosed. A manipulation surface locates the fluid sample. A surface acoustic wave (SAW) generation material layer is provided. This is a polycrystalline material, textured polycrystalline material, biaxially textured polycrystalline material, microcrystalline material, nanocrystalline material, amorphous material or composite material. A transducer electrode structure arranged at the SAW generation material layer provides SAWs at the manipulation surface for interaction with the fluid sample. The manipulation surface has a phononic structure, for affecting the transmission, distribution and/or behaviour of SAWs at the manipulation surface. The apparatus is typically manufactured by reel-to-reel processes, to reduce the unit cost to a level at which the apparatus can be considered to be disposable after a single use. |
| IP Reference | CN103492078 |
| Protection | Patent granted |
| Year Protection Granted | 2014 |
| Licensed | No |
| Impact | Background IP for spin out |
| Title | SAMPLE NEBULIZATION |
| Description | A device for generating a nebulized sample for detection of an analyte. The device includes a surface acoustic wave (SAW) transducer and a superstrate. The superstrate has a first surface for coupling with the SAW transducer and a second surface for receiving a fluid sample incorporating the analyte. The fluid sample is nebulized from the second surface. The superstrate is provided with an electrical connection extending from the second surface of the superstrate to provide a conducting path from a charge source to the second surface of the superstrate. The charge source may be the surface of the transducer or an external voltage source. |
| IP Reference | US2014083174 |
| Protection | Patent granted |
| Year Protection Granted | 2014 |
| Licensed | No |
| Impact | Enables leverage with other companies |
| Title | Approximate Bayesian Computation (ABC) |
| Description | Parameter inference algorithm for stochastic simulations |
| Type Of Technology | Software |
| Year Produced | 2013 |
| Impact | Use of this algorithm has lead to a collaboration with other academics within Imperial college with the work hopefully leading to a joint paper. |
| Title | Capture Assay Model |
| Description | A software that models the scaling advantages and constraints in miniaturized capture assays for single cell proteomics |
| Type Of Technology | Software |
| Year Produced | 2013 |
| Impact | Used in group to predict assay behaviour and inform assay validation |
| Title | Humidity cell for 2DIR experiments |
| Description | A humidity cell was made from a cheap optical mount, a CaF2 window, a glass coverslip, PTFE spacers and a rubber o-ring. The biological sample of interest is placed on the glass slide along with several drops of saturated salt solution, which enable a constant humidity to be maintained inside the cell for at least a month. This ensures that the water content of the biological sample of interest remains constant throughout a series of 2DIR experiments. |
| Type Of Technology | Physical Model/Kit |
| Year Produced | 2014 |
| Impact | This humidity cell is much smaller than commercial humidity cells and is easier to mount on our 2DIR sample stage, allowing for a quicker and more reproducible experimental set-up. It is also much cheaper than commercial cells, which means that we have made 5 of these humidity cells containing different biological samples for the same price as just 1 commercial cell. This leads to higher throughput in our 2DIR experiments. |
| Title | Plasmids tagging VHR with HA and Flag |
| Description | Plasmids pcDNA-3.1-Flag-VHR and pcDNA-3.1-HA-VHR, to express N-terminal tagged VHR. |
| Type Of Technology | New Material/Compound |
| Year Produced | 2014 |
| Impact | Enabled tagged VHR to be expressed in cell lines and detected using different antibodies |
| Title | TIRF-FLIM system |
| Description | Combined total internal reflection fluorescence lifetime imaging system for improved resolution to conventional fluorescence lifetime imaging techniques |
| Type Of Technology | New/Improved Technique/Technology |
| Year Produced | 2014 |
| Impact | Enhanced the capabilities of standard fluorescence lifetime imaging through improved axial resolution. Technology is of particular use for investigations of dynamics in biological membranes. |
| Company Name | SAW Dx |
| Description | "SAW Dx is an emerging point of care diagnostic (POC) device design and manufacturing venture focused on exploiting Acoustic-Flow technology based on the use of surface acoustic waves technology developed in the laboratory of Prof Jon Cooper, University of Glasgow. The Acoustic-Flow technology utilises the interaction of Surface Acoustic Waves (SAW) with a patient sample to drive a diagnostic test. The interactions are controlled by microstructures patterned on the surface of disposable chips, to shape sound in particular ways in a frequency dependant manner. Functions possible include fluid movement, mixing, particle patterning, enrichment, opening cells to release DNA and temperature cycling of a sample. Acoustic-Flow technology means that samples can be processed on-chip (no preprocessing required) and complex micro-fluidics are avoided. The SAW Dx business model is based on providing product and technology solutions to life sciences and diagnostics companies seeking to enhance their diagnostic product offerings through innovative point of care use. " |
| Year Established | 2013 |
| Impact | SAW Dx is involved in 3 innovateUK projects, partnering with clinicians and manufacturing companies, focussed on developing point-of-care diagnostics for TB, animal health, and sexually transmitted diseases |
| Description | 'FedEx delivery for single cells' - Using HOT to develop the world's most accurate single cell delivery system, Joint CDT conference, Warwick University |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | keynote/invited speaker |
| Geographic Reach | Regional |
| Primary Audience | Postgraduate students |
| Results and Impact | Student led conference at the University of Warwick. The speaker, Douglas Wylie, received the second place prize for student talk at this event. n/a |
| Year(s) Of Engagement Activity | 2014 |
| Description | Biological strategies for the investigation of proteomes, Research in progress session, Aston University |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | keynote/invited speaker |
| Geographic Reach | Local |
| Primary Audience | Professional Practitioners |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2013 |
| Description | British Society of Rheology midwinter meeting |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Other audiences |
| Results and Impact | Talk delivered at the British Society of Rheology midwinter meeting on Microfluidics and ultrasonics. |
| Year(s) Of Engagement Activity | 2015 |
| Description | British Society of Rheology midwinter meeting |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Other audiences |
| Results and Impact | Talk delivered at the British Society of Rheology midwinter meeting on Microfluidics and ultrasonics. |
| Year(s) Of Engagement Activity | 2015 |
| Description | Clinical Needs and Sensing Solutions brainstorming session |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Public/other audiences |
| Results and Impact | This talk was a brainstorming session that involved discussions about how to integrate developed tools and technologies in societal, mainly healthcare, needs. Possible collaborations were discussed. |
| Year(s) Of Engagement Activity | 2012 |
| Description | Optical Manipulation of Chemically Functionalised Emulsions and Suspensions for Single Cell Analysis, OSA Optics in Life Sciences Conference, Hawaii |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | keynote/invited speaker |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2013 |
| Description | Technologies to study protein oxidation in ageing. Life and Health Sciences Research day, Aston University |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | poster presentation |
| Geographic Reach | Local |
| Primary Audience | Postgraduate students |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2012 |
| Description | Two-Dimensional Infrared Spectroscopy as a Potential Tool for Tissue Imaging, SPEC 2014: Shedding New Light on Disease, Krakow, Poland |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | poster presentation |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2014 |
| Description | A doctrine of doctors |
| Form Of Engagement Activity | A magazine, newsletter or online publication |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Media (as a channel to the public) |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2014 |
| Description | A first step towards practical single cell proteomics: a microfluidic antibody capture chip with TIRF detection, Brompton Respiratory BRU meeting, Imperial College London |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Professional Practitioners |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2013 |
| Description | Acoustic Microfluidics - What a sound idea |
| Form Of Engagement Activity | A magazine, newsletter or online publication |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Media (as a channel to the public) |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2012 |
| Description | Acoustofluidics 2014, Monash University Prato Centre, Italy |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | keynote/invited speaker |
| Geographic Reach | International |
| Primary Audience | Postgraduate students |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2014 |
| Description | Adrenalin-fuelled creativity |
| Form Of Engagement Activity | A magazine, newsletter or online publication |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Media (as a channel to the public) |
| Results and Impact | Article stimulated thinking about possibilites academia. n/a |
| Year(s) Of Engagement Activity | 2012 |
| Description | Advances in Chromatography article for Chromatography Today magazine |
| Form Of Engagement Activity | A magazine, newsletter or online publication |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | Magazine helped promote research N/A |
| Year(s) Of Engagement Activity | 2015 |
| Description | Advantages and Limitations of Mass Spectrometry as a Diagnostic Toolfor Protein Oxidation in Redox Biology, SFRBM conference, Seattle 2014 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Other academic audiences (collaborators, peers etc.) |
| Results and Impact | Best practice shared Teach young researchers best practice |
| Year(s) Of Engagement Activity | 2014 |
| URL | http://www.sfrbm.org/ |
| Description | Althea-Imperial Programme 2014/15 |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Other academic audiences (collaborators, peers etc.) |
| Results and Impact | Discussions and actions culminated in the department getting the Athena Swan Gold award. n/a |
| Year(s) Of Engagement Activity | 2014 |
| Description | Analysis of PTEN protein-protein interactions in altered redox conditions. Research in progress seminar, Aston University |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | keynote/invited speaker |
| Geographic Reach | Local |
| Primary Audience | Professional Practitioners |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2014 |
| Description | Analysis of PTEN protein-protein interactions in its reduced and oxidized form by GSH affinity enrichment and MS-based label free protein quantitation, SFRRI Meeting, Kyoto, Japan |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | poster presentation |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2014 |
| Description | Analysis of PTEN protein-protein interactions in its reduced and oxidized form by GSH affinity enrichment and MS-based label free protein quantitation. Postgraduate Research day, Aston University |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | keynote/invited speaker |
| Geographic Reach | Local |
| Primary Audience | Postgraduate students |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2014 |
| Description | Biochemistry of Redox Reactions. SFRR-Europe Summer School 2012 on Dietary factors and redox signalling, Spetses, Greece |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | keynote/invited speaker |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2012 |
| Description | Biomolecule Oxidation: Biological Effects and Biomarkers 2015 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Other academic audiences (collaborators, peers etc.) |
| Results and Impact | UK-Denmark interactions started Potential for collaboration discussed |
| Year(s) Of Engagement Activity | 2015 |
| Description | COST CM1001 Workshop on Mass Spectrometry Analysis of Protein Oxidation |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Other academic audiences (collaborators, peers etc.) |
| Results and Impact | This workshop was very interesting and was very successful in generating new ideas and disseminating information. New contacts were made for possible future collaborations. |
| Year(s) Of Engagement Activity | 2013 |
| Description | CREST Award (British Science Association) |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2014 |
| Description | Café Scientifique Liverpool |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Public/other audiences |
| Results and Impact | Public talk as part of the British Science Association's Café Scientifique programme on interdisciplinary research and biophotonics N/A |
| Year(s) Of Engagement Activity | 2015 |
| URL | http://www.cafescientifique.org/index.php?option=com_contentbuilder&title=uk-liverpool&controller=de... |
| Description | Capillary zone electrophoresis coupled with an immunoassay for protein identification, Institute of Chemical Biology MRes Conference, Imperial College London |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | keynote/invited speaker |
| Geographic Reach | Local |
| Primary Audience | Postgraduate students |
| Results and Impact | Student research talk performed at the end of the masters year to the entire cohort. n/a |
| Year(s) Of Engagement Activity | 2012 |
| Description | Challenges and successes in analysing adducts between proteins and phospholipid oxidation products, COST CM1001 Workshop, The Hebrew University, |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | keynote/invited speaker |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2013 |
| Description | Characterisation of tyrosine nitration by two-dimensional infrared spectroscopy, 204th Infrared and Raman Discussion Group (IRDG) Meeting , University College London |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | poster presentation |
| Geographic Reach | Regional |
| Primary Audience | Professional Practitioners |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2013 |
| Description | Characterisation of tyrosine nitration by two-dimensional infrared spectroscopy, Chemistry PostGrad symposium, Imperial College London |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | poster presentation |
| Geographic Reach | Local |
| Primary Audience | Postgraduate students |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2014 |
| Description | Chemical tools to probe phosphoinositide dependent signalling, Membrane, Morphology and Function, Fara San Martino, Abruzzo, Italy |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | keynote/invited speaker |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2014 |
| Description | Continuous Ultrasonic Nebulisation Transfer for Mass Spectrometry using a Phononic Crystal Superstrate, British Mass Spectroscopy Society annual meeting |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | poster presentation |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2013 |
| Description | Dara O'Briain's Science Club |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | Yes |
| Geographic Reach | National |
| Primary Audience | Media (as a channel to the public) |
| Results and Impact | Scientific Advice given to the TV show that has resulted in scientifically correct experiments and information being broadcasted on the BBC. n/a |
| Year(s) Of Engagement Activity | 2012 |
| Description | Design, Synthesis and Application of Chemical Biology Probes for Measuring Oxidative Protein Damage, Joint CDT conference, Warwick University |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | keynote/invited speaker |
| Geographic Reach | Regional |
| Primary Audience | Postgraduate students |
| Results and Impact | Talk at a student led conference at the University of Warwick n/a |
| Year(s) Of Engagement Activity | 2014 |
| Description | Design, synthesis and activity profiling of modified 3-and 5-hydroxypicolinic acid vanadyl complexes against PTEN as models for targeted probes, Institute of Chemical Biology MRes Conference, Imperial College London |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | keynote/invited speaker |
| Geographic Reach | Local |
| Primary Audience | Postgraduate students |
| Results and Impact | Student research talk performed at the end of the masters year to the entire cohort. n/a |
| Year(s) Of Engagement Activity | 2012 |
| Description | Design, synthesis and biological evaluation of fluorescent vanadyl complexes - towards the development of targeted probes for monitoring oxidative damage, Chemistry PostGrad symposium |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | poster presentation |
| Geographic Reach | Local |
| Primary Audience | Postgraduate students |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2014 |
| Description | Detection and quantification of protein oxidation in sarcopenic models: a mass spectrometry study, LHS Postgraduate Research Day, Aston University, UK, |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | keynote/invited speaker |
| Geographic Reach | Local |
| Primary Audience | Postgraduate students |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2014 |
| Description | Detection and quantification of protein oxidation in sarcopenic models: a mass spectrometry study, LHS Postgraduate Research Day, Aston University, UK, |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | keynote/invited speaker |
| Geographic Reach | Local |
| Primary Audience | Postgraduate students |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2014 |
| Description | Detection and quantification of protein oxidation in sarcopenic models: a mass spectrometry study, Life beyond the PhD, Cumberland lodge, UK |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | keynote/invited speaker |
| Geographic Reach | Regional |
| Primary Audience | Postgraduate students |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2014 |
| Description | Detection and quantification of protein oxidation in sarcopenic models: a mass spectrometry study, Life beyond the PhD, Cumberland lodge, UK |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Postgraduate students |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2014 |
| Description | Detection and quantification of protein oxidation in sarcopenic models: a mass spectrometry study. SFRR-E meeting 2014, Campus des Cordeliers, Paris |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | poster presentation |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2014 |
| Description | Developing Diagnostics for the Developing World, SPIE Photonics West, San Francisco |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | keynote/invited speaker |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2012 |
| Description | EVV 2DIR Spectroscopy for the detection and analysis of Biomolecular complexes, geometry/structure determination and analytical studies of proteins and metabolites, School of Chemistry, University of Nottingham |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | keynote/invited speaker |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2013 |
| Description | Electron-Vibration-Vibration coupling as readout signature for biomolecular composition, structure and dynamics, Joint Meeting of the British and German Biophysical Society, Hünfeld, Germany |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | keynote/invited speaker |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2013 |
| Description | FameLab masterclass |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Public/other audiences |
| Results and Impact | Talk was received very well and many discussions were held afterwards. n/a |
| Year(s) Of Engagement Activity | 2013 |
| Description | FedEx delivery for single cells using 'HOT' - Holographic Optical Trapping as a tool for quantifiable cell biology, Chemistry PostGrad symposium, Imperial College London |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | poster presentation |
| Geographic Reach | Local |
| Primary Audience | Postgraduate students |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2014 |
| Description | Glasgow Explorathon |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Public/other audiences |
| Results and Impact | Stand and presentations were received very well and many questions and thoughts were shared with our scientific outreach team. n/a |
| Year(s) Of Engagement Activity | 2014 |
| Description | Glasgow Science Centre |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Public/other audiences |
| Results and Impact | The table top presentations sparked interest in the public and we received many questions about our research. The Glasgow Science Centre loved our presentation and is looking forward to host our outreach activities again. |
| Year(s) Of Engagement Activity | 2014 |
| Description | Harnessing mass spectrometry to investigate oxidative modifications of proteins. STRESS IN HEALTH AND DISEASE IV SPANISH AND PORTUGUESE MEETING ON FREE RADICALS VALENCIA |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | keynote/invited speaker |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2012 |
| Description | Imperial Fringe |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Public/other audiences |
| Results and Impact | Talk sparked interest and got advice for further outreach event presentations. n/a |
| Year(s) Of Engagement Activity | 2013 |
| Description | Interfacing surface acoustic wave nebulisation with LC-MS |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Other academic audiences (collaborators, peers etc.) |
| Results and Impact | good feed back Informed international audience of SAWN LC/MS |
| Year(s) Of Engagement Activity | 2015 |
| Description | International Conference on Ultrasonics |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Postgraduate students |
| Results and Impact | Talk delivered by PhD student Ben Tiller at the annual congress on ultrasonics in Metz France. Discussed current research with a variety of academics. |
| Year(s) Of Engagement Activity | 2015 |
| Description | Interview with Andy Roast |
| Form Of Engagement Activity | A magazine, newsletter or online publication |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Media (as a channel to the public) |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2012 |
| Description | Invited Talk at 8th International Conference on Advanced Vibrational Spectroscopy (ICAVS8), 12-17 July 2015, Vienna, Austria |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Scientific results were presented to a large audience and this led to interesting questions and discussions Increased the awareness of the EVV 2DIR technique and the important scientific challenges to which it can be applied |
| Year(s) Of Engagement Activity | 2015 |
| Description | Lipid oxidation products: signalling and biomarkers for inflammatory disease. Oxygen Club of California World Congress 2012 on OXIDANTS AND ANTIOXIDANTS IN BIOLOGY: CELL SIGNALING AND NUTRIENT - GENE INTERACTIONS, 20-23 June 2012, Alba, Italy |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | keynote/invited speaker |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2012 |
| Description | London Sci-Fi Convention |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Public/other audiences |
| Results and Impact | The stand at the SciFi Convention received a lot of interest from pupils to older people. People were fascinated by the presented science and the possibilities it offers. Conference organiser thought our outreach stand and table-top demos were one of the highlights of the convention and looks forward to having more scientific attendees/presenters in future events. |
| Year(s) Of Engagement Activity | 2014 |
| Description | Magnetide-doped PDMS as a solid phase support for phosphopeptides |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Other academic audiences (collaborators, peers etc.) |
| Results and Impact | Informed international audience of the development of a new enrichment strategy Very good feed back |
| Year(s) Of Engagement Activity | 2014 |
| Description | Mapping oxidative modifications of PTEN, SFRRI International Meeting, Imperial College London |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | poster presentation |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2012 |
| Description | Mass Spectrometry Analysis of Protein Oxidation: Basic Principles and Application to Neutrophil Extracellular Traps. Birmingham Dental School, |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Professional Practitioners |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2012 |
| Description | Mass spectrometry analysis of modified bee venom phospholipase A2 in correlation with increased activity, SFRRI International Meeting, Imperial College London |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | poster presentation |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2012 |
| Description | Mass spectrometry and proteomic approaches to identity oxidative protein modifications, Aston University |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | keynote/invited speaker |
| Geographic Reach | Local |
| Primary Audience | Professional Practitioners |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2013 |
| Description | Mass spectrometry and proteomic approaches to identity oxidative protein modifications, COST CM1001 Workshop, The Hebrew University |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | keynote/invited speaker |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2013 |
| Description | Mass-spectrometry as a diagnostic platform for oxidative modification of proteins. Life and Health Sciences Research day, Aston University |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | poster presentation |
| Geographic Reach | Local |
| Primary Audience | Postgraduate students |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2012 |
| Description | Metals in medicine: Diagnostic and therapeutic agents |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Other academic audiences (collaborators, peers etc.) |
| Results and Impact | Presentation was very successful and culminated in many questions asked. n/a |
| Year(s) Of Engagement Activity | 2013 |
| Description | Microfluidic Antibody Capture Chips: Battling Cancer One Cell at a Time, Chemistry PostGrad symposium, Imperial College London |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | poster presentation |
| Geographic Reach | Local |
| Primary Audience | Postgraduate students |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2014 |
| Description | Microfluidic Antibody Capture Chips: Battling Cancer One Cell at a Time, Joint CDT Conference, Warwick University |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | keynote/invited speaker |
| Geographic Reach | Regional |
| Primary Audience | Postgraduate students |
| Results and Impact | Talk at a student led conference at the University of Warwick. The speaker, Michael Barclay, won the first place talk prize at this event n/a |
| Year(s) Of Engagement Activity | 2014 |
| Description | Microfluidic-based single molecule counting approaches to diagnostics, Novartis external seminar program, Novartis, Basel, |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | keynote/invited speaker |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2012 |
| Description | Microfluidics and Miniaturised Assays for Single Cell Analysis: Applications to Basic Biology and Clinical Challenges, Royal Society of Chemistry Analytical Research Forum, London |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | keynote/invited speaker |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2014 |
| Description | Miniaturisation of Technologies Measurement and Impact on Healthcare |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Professional Practitioners |
| Results and Impact | The talk finished with interesting audience questions and further discussions were led at the end of the event. Possibilities for collaborative work were discussed after the meeting. |
| Year(s) Of Engagement Activity | 2012 |
| Description | More than just 'stinks': Using chemical tools to unlock biology' |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Schools |
| Results and Impact | Talk stimulated thinking in 6th form students who were excited to see the possibilities in scientific research. n/a |
| Year(s) Of Engagement Activity | 2012 |
| Description | Multidisciplinary research colloquium |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Participants in your research and patient groups |
| Results and Impact | Organised event; gave final talk New collaborations developed; resource and idea sharing |
| Year(s) Of Engagement Activity | 2015 |
| Description | Multiplexed Infectious Disease Testing - Sound Point-of-Care Diagnostics, Lab-on-a-Chip European Congress, Berlin, Germany |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | keynote/invited speaker |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2014 |
| Description | Networking and Collaborations |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Other academic audiences (collaborators, peers etc.) |
| Results and Impact | The talk finished with interesting audience questions and further discussions were led at the end of the event. Talk sparked interest and got advice for further outreach event presentations. |
| Year(s) Of Engagement Activity | 2013 |
| Description | New Vanadium Complexes as Optical Probes to Detect Cys Sulfenic Modifications in PTEN, EuroBIC 12, Zurich, Switzerland |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | poster presentation |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2014 |
| Description | Novel Micro-delivery systems: Through live single cell membranes and beyond, Institute of Chemical Biology MRes Conference, Imperial College London |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | keynote/invited speaker |
| Geographic Reach | Local |
| Primary Audience | Postgraduate students |
| Results and Impact | Student research talk performed at the end of the masters year to the entire cohort. n/a |
| Year(s) Of Engagement Activity | 2012 |
| Description | Novel Tools and Technologies for Single Cell Proteomics Analysis, Single Cell Analysis Conference, Cambridge, Massachusetts, USA, |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | keynote/invited speaker |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2014 |
| Description | Novel nanodelivery systems to single cell membranes and beyond, Photon12, Durham |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | poster presentation |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2012 |
| Description | Novel nanodelivery systems to single cell membranes and beyond, SFRRI International Meeting, Imperial College London |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | poster presentation |
| Geographic Reach | Local |
| Primary Audience | Professional Practitioners |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2012 |
| Description | Oxidation of proteins: mechanisms and role in signalling, methods for analysis of protein oxidation & Oxidative Stress in inflammation and atherosclerosis |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Other academic audiences (collaborators, peers etc.) |
| Results and Impact | These lecture series were very well received. n/a |
| Year(s) Of Engagement Activity | 2013 |
| Description | Oxidized phospholipids in the vascular system and their cellular effects, X Meeting of the Spanish Group for Research on Free Radicals (Geirli) Symposium on oxidative stress and redox signalling in biology and medicine, Aula Magna, Faculty of Medicine, |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | keynote/invited speaker |
| Geographic Reach | International |
| Primary Audience | Postgraduate students |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2014 |
| Description | Oxidized proteins in chronic disease: how to detect them and why they are important, ICA-Symposium Summer 2014, Salzburg |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | keynote/invited speaker |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2014 |
| Description | PH-domain mimetics: potential drugs for Lowe Syndrome?, 4th international Symposium of the Lowe syndrome Trust - Molecular and clinical advances in Lowe Syndrome, Royal Society London |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | keynote/invited speaker |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2012 |
| Description | PRINCE2 Project Management Overview Training Course |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Other academic audiences (collaborators, peers etc.) |
| Results and Impact | Dr Julia Davies gained an understanding of project management and would like to pursue this as a career Dr Julia Davies will enrol on a PRINCE2 training course and gain qualifications |
| Year(s) Of Engagement Activity | 2015 |
| Description | Pleckstrin homology domain mimicry: chemical biology of phosphoinositide signalling, VIPCA conference on Chemical Biology: Methods and Progress, Vienna University, Austria |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | keynote/invited speaker |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2013 |
| Description | Poster Presentation at IRDG Xmas Meeting 2014 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Other academic audiences (collaborators, peers etc.) |
| Results and Impact | Poster was well received and initiated interesting discussions I have been asked to give a talk at the IRDG Xmas Meeting 2015 |
| Year(s) Of Engagement Activity | 2014 |
| Description | Poster presented at HUPO2014. Title: The Redox Interactome of PTEN: Cellular and Protein Specific Changes |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Other academic audiences (collaborators, peers etc.) |
| Results and Impact | Presented research methods and findings Positive feedback from other academics and stimulating discussion |
| Year(s) Of Engagement Activity | 2014 |
| Description | Programmable light |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Public/other audiences |
| Results and Impact | The talk finished with interesting audience questions n/a |
| Year(s) Of Engagement Activity | 2012 |
| Description | Progress Towards Point of Care Methods for Single Cell Protein Analysis, 2nd Annual Single Cell Genomics & Transcriptomics Asia Congress 2014, Singapore |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | keynote/invited speaker |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2014 |
| Description | Progress in Deconvolving Structural Elements In a Small Protein Using EVV 2DIR. 6th International Conference on Coherent Multidimensional Spectroscopy |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2012 |
| Description | Project Management |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Professional Practitioners |
| Results and Impact | Talk was received very well and many discussions were held afterwards. n/a |
| Year(s) Of Engagement Activity | 2013 |
| Description | Protein Oxidative Modifications: importance in pathophysiology and measurement. Proteomics Workshop 2012 Aveiro, Portugal |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | keynote/invited speaker |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2012 |
| Description | Protein modification and phospholipid oxidation. Society for Free Radical Research Europe 2013 Conference Athens |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | keynote/invited speaker |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2013 |
| Description | Protein oxidation in stress and disease - finding the needle in the haystack. Analytical methods to study oxidative damage, antioxidants & drugs (CONFERENCE). Medical University of Bialystok |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | keynote/invited speaker |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2011 |
| Description | Protein oxidation in stress and disease - finding the needle in the haystack. Society for Free Radical Research Europe 2011 Conference Istanbul |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | keynote/invited speaker |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2011 |
| Description | Proteome-on-a-chip: Automation of Protein Separation, Deposition, and Readout of Oxidative Damage, Joint CDT Multidisciplinary conference, Royal Academy of Engineering |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | poster presentation |
| Geographic Reach | Regional |
| Primary Audience | Postgraduate students |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2013 |
| Description | Proteomic profiling of oxidations of muscle proteins in disease, Research in Progress Seminar, Aston University |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | keynote/invited speaker |
| Geographic Reach | Local |
| Primary Audience | Professional Practitioners |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2014 |
| Description | Quantitative Single Cell and Single Molecule Proteomics for Clinical Studies, Cambridge Healthtech Institute's Second Annual Quantitative Digital Detection Technologies conference, Boston |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | keynote/invited speaker |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2014 |
| Description | Quantitative Single Cell and Single Molecule Proteomics for Clinical Studies. Institute of Virology, Kyoto University, Japan |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | keynote/invited speaker |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2013 |
| Description | RSC Chemistry World A Doctrine of Doctors |
| Form Of Engagement Activity | A magazine, newsletter or online publication |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Other academic audiences (collaborators, peers etc.) |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2014 |
| Description | Research in Progress seminar, Aston University |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Professional Practitioners |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2012 |
| Description | Royal Society Summer Science Exhibition |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Public/other audiences |
| Results and Impact | Annual event that takes place at the Royal Society in London. Around 20 groups from around the UK and beyond are selected to take part in this prestigious event. The event involved constructing a stand and a series of interactive demos. This was split across the three institutions, Imperial, Glasgow and Aston with each institution creating one demo or activity. The event spanned an entire week which involved interacting with secondary school students, members of the general public and fellows of the Royal Society. Since the event we have a number of requests for more detailed information which related to our stand. This included an A-Level student asking for information about oxidative damage for a school presentation and a student at the Royal College of Art asking for information about how sound waves can be used to interact with biological material. |
| Year(s) Of Engagement Activity | 2015 |
| URL | http://sse.royalsociety.org/2015 |
| Description | Salter's Chemistry Challenge |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Schools |
| Results and Impact | Presentation was very successful and culminated in many questions asked. Pupils were very excited by the talk and asked many intriguing questions. |
| Year(s) Of Engagement Activity | 2013 |
| Description | Sarcoplasmic reticulum protein as models of protein oxidation in ageing. Life and Health Sciences Research day, Aston University |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | poster presentation |
| Geographic Reach | Local |
| Primary Audience | Postgraduate students |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2014 |
| Description | Sarcoplasmic reticulum protein as models of protein oxidation in ageing. Research in Progress seminar, Aston University |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Professional Practitioners |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2012 |
| Description | SciBar Liverpool |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Public/other audiences |
| Results and Impact | Proxomics member took part in a meet up in a bar in Liverpool that occurs monthly. Around 50 people usually attend to listen to talks about various scientific topics. The event got regular members of the public and students interested and engaged with biophotonics. |
| Year(s) Of Engagement Activity | 2015 |
| URL | http://www.cafescientifique.org/index.php?option=com_contentbuilder&title=uk-liverpool-scibar&contro... |
| Description | Sense About Science |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Public/other audiences |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2013 |
| Description | Sense About Science Peer Review Workshop |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Public/other audiences |
| Results and Impact | Discussions led to improve outreach program. n/a |
| Year(s) Of Engagement Activity | 2013 |
| Description | Single Cell Analysis of Proteins from Clinical Samples Relevant to Human Health, HUPO Conference, Yokohama, Japan |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | keynote/invited speaker |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2013 |
| Description | Single Cell Analysis: A new frontier for Clinical Research and Clinical Practise; Measuring protein copy number and variation in a single cell, High Throughput/High Content Technologies Community, Imperial College London |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | keynote/invited speaker |
| Geographic Reach | Local |
| Primary Audience | Postgraduate students |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2012 |
| Description | Single Cell Distributions: Investigating the effect of Actinomycin D on p53 at the single cell level with the use of Microfluidic Antibody Capture (MAC) devices, Institute of Chemical Biology MRes Conference, Imperial College London |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | keynote/invited speaker |
| Geographic Reach | Local |
| Primary Audience | Postgraduate students |
| Results and Impact | Student research talk performed at the end of the masters year to the entire cohort. n/a |
| Year(s) Of Engagement Activity | 2012 |
| Description | Single Cell Protein Analysis from Clinical Samples Relevant to Human Health, Single Cell Genomics and Transcriptomics Asia Congress, Singapore |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | keynote/invited speaker |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2013 |
| Description | Single Cell Protein Analysis: Potential for impact on clinical research and clinical outcomes, ITMAT 9th Annual International Symposium, Perelman School of Medicine, University of Pennsylvania |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | keynote/invited speaker |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2014 |
| Description | Single Cell Proteomics: Understanding Cancer One Cell at a Time, Point-of-Care Diagnostics workshop, 4 December 2012, Imperial College London |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | poster presentation |
| Geographic Reach | Regional |
| Primary Audience | Professional Practitioners |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2012 |
| Description | Single Cell Proteomics: Understanding Cancer One Cell at a Time, SET for Britain 2013 (final), House of Commons, Westminster |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Policymakers/politicians |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2013 |
| Description | Small-Molecule Optical Probes for Fluorescence Imaging of Inflammation through FPRs. William Harvey Research Institute (WHRI), Queen Mary University, London |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | keynote/invited speaker |
| Geographic Reach | Regional |
| Primary Audience | Professional Practitioners |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2014 |
| Description | Smart Droplet Microtools: Probing a single cell using "optical tweezers", SET for Britain 2012, Terrace Marquee, Houses of Parliament |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Policymakers/politicians |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2012 |
| Description | Spectroscopy in a suitcase |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Schools |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2014 |
| Description | St. Edward's Catholic Primary School Summer Fair |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Schools |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2014 |
| Description | Study of protein-tyrosine nitration by two-dimensional infrared (2DIR) spectroscopy, Institute of Chemical Biology MRes Conference, Imperial College London |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | keynote/invited speaker |
| Geographic Reach | Local |
| Primary Audience | Postgraduate students |
| Results and Impact | Student research talk performed at the end of the masters year to the entire cohort. n/a |
| Year(s) Of Engagement Activity | 2012 |
| Description | Study of protein-tyrosine nitration by two-dimensional infrared vibrational spectroscopy, SFRRI International Meeting, Imperial College London |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | poster presentation |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2012 |
| Description | Surface Acoustic Wave Nebulisation on Structured Superstrates, Single Cell Analysis Europe Conference, Berlin, Germany |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | poster presentation |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2014 |
| Description | Symposium at SFRRE 2014 |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Other academic audiences (collaborators, peers etc.) |
| Results and Impact | Teams from US and Europe brought together New collaborations established |
| Year(s) Of Engagement Activity | 2014 |
| URL | https://www.sfrr-europe.org/ |
| Description | Talk at Imperial Science Foresight 2035 Conference |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Postgraduate students |
| Results and Impact | Invited talk by PhD student within the group to discuss their research and project the outcome of the work 30 years into the future. |
| Year(s) Of Engagement Activity | 2015 |
| Description | Targetted mass spectrometry methods for detecting oxPTMs relevant to ageing and epigenetics. 5th International Symposium on Nutrition, Oxygen Biology & Medicine, UPMC Campus des Cordeliers, Paris |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | poster presentation |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2013 |
| Description | Targetted mass spectrometry methods for detecting oxPTMs. SFRR-E meeting 2014, Campus des Cordeliers, Paris |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | poster presentation |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2014 |
| Description | Targetted mass spectrometry methods for detecting oxPTMs., HUPO 5-8 October 2014, Madrid |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | poster presentation |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2014 |
| Description | Technologies to study protein oxidation in ageing. Postgraduate Research day, Aston University |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | keynote/invited speaker |
| Geographic Reach | Local |
| Primary Audience | Postgraduate students |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2014 |
| Description | The Why, How and What of Single Celll Analysis: Towards Routine Analysis of Human Tissue Relevant to Human Health, Conway Institute, University College Dublin, Dublin, Ireland |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | keynote/invited speaker |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2014 |
| Description | The proteomics of oxidation: optical tools to probe the microscale, Brompton Respiratory BRU meeting, Imperial College London |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Professional Practitioners |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2013 |
| Description | The road ahead: from idea to implementation |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Other academic audiences (collaborators, peers etc.) |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2014 |
| Description | ThinkTank 'meet the expert' |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Public/other audiences |
| Results and Impact | Inspired young children to be interest in Science Very good feed back from ThinkTank |
| Year(s) Of Engagement Activity | 2015 |
| URL | http://www.birminghammuseums.org.uk/thinktank/whats-on/meet-the-experts |
| Description | Towards Practical Single Cell Proteomics: A Microfluidic Antibody Capture Chip with TIRF Detection, SFRRI International Meeting, Imperial College London |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | poster presentation |
| Geographic Reach | Regional |
| Primary Audience | Professional Practitioners |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2012 |
| Description | Towards Single Cell Analysis of Proteins from Clinical Samples Relevant to Human Health, Single Cell Analysis Europe Conference, Berlin, Germany |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | keynote/invited speaker |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2014 |
| Description | Using 2DIR spectroscopy to probe tyrosine nitration, Joint CDT Conference, Warwick University |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | keynote/invited speaker |
| Geographic Reach | Regional |
| Primary Audience | Postgraduate students |
| Results and Impact | Talk at a student led conference at the University of Warwick n/a |
| Year(s) Of Engagement Activity | 2014 |
| Description | Using Phononic Crystals to Shape Complex Fluid Flows, Lab-on-a-Chip European Congress, Barcelona, Spain |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | keynote/invited speaker |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2013 |
| Description | Using microfluidics to monitor oxidative stress response on a single cell level, Microfluidics 2014, Heidelberg, Germany |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | poster presentation |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2014 |
| Description | Workshop at SFRBM |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Other academic audiences (collaborators, peers etc.) |
| Results and Impact | Best practice shared Taught young researchers best practice |
| Year(s) Of Engagement Activity | 2014 |
| URL | http://www.sfrbm.org/ |
| Description | mart Droplet Microtools: an optically controlled platform for single- and sub-cellular surgery, International Biophysics Congress 2014, Brisbane, Australia |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | poster presentation |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | n/a |
| Year(s) Of Engagement Activity | 2014 |
| Description | £4.6m project aims to understand causes of ageing |
| Form Of Engagement Activity | A magazine, newsletter or online publication |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Media (as a channel to the public) |
| Results and Impact | Local interest raised in the project. n/a |
| Year(s) Of Engagement Activity | 2012 |