Development of an optical system for on-line tracking of cell growth on microcarriers

Lead Research Organisation: University of Huddersfield
Department Name: Sch of Computing and Engineering

Abstract

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Publications

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Odeleye AOO (2017) Development of an optical system for the non-invasive tracking of stem cell growth on microcarriers. in Biotechnology and bioengineering

 
Description The key achievements of this award have been in the development and demonstration of dedicated research apparatus in the form of a submersible interference microscope capable of imaging moving micro-carrier in-situ within an environment representing a stirred tank bioreactor. Image analysis routines to enable cell counting on populated micro-carriers were developed based upon a combination of: Hough circle detection and cropping, Laplacian of Gaussian filtering and thresholding. The method was validated using images obtained from the interferometry system on bone-marrow derived human mesenchymal stem cells grown on a range of micro-carrier types. Work towards dynamic imaging of moving microcarriers in-situ was also carried out using a high power pulsed laser source. At the time of writing an academic paper is under preparation to report the initial results. A further one is planned on results of dynamic imaging.
Exploitation Route The research outcomes for this feasibility study have been promising enough that the collaboration between The Centre for Biological Engineering, Loughborough and the Centre for Precision Technologies, University of Huddersfield is ongoing. The work related to the original funded project objectives is being continued through MSc project and spare post-graduate researcher capacity as the developed research apparatus is fully operational. It is fully anticipated that further results will lead to a strong case for more RCUK funded support in the future and the project team are actively pursuing this route at the time of writing.
At the time of submission a paper summarising the key findings is under the final stages of peer review with the journal, Biotechnology & Bioengineering.
Sectors Healthcare,Manufacturing, including Industrial Biotechology,Pharmaceuticals and Medical Biotechnology