New Enzymatically Produced Interpenetrating Starch-Cellulose Gels
Lead Research Organisation:
John Innes Centre
Department Name: Biological Chemistry
Abstract
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People |
ORCID iD |
| Rob Field (Principal Investigator) |
Publications
Bulmer GS
(2021)
Recent advances in enzymatic synthesis of ß-glucan and cellulose.
in Carbohydrate research
Dedola S
(2020)
Revisiting the Language of Glycoscience: Readers, Writers and Erasers in Carbohydrate Biochemistry.
in Chembiochem : a European journal of chemical biology
Gabrielli V
(2021)
Molecular Recognition of Natural and Non-Natural Substrates by Cellodextrin Phosphorylase from Ruminiclostridium Thermocellum Investigated by NMR Spectroscopy.
in Chemistry (Weinheim an der Bergstrasse, Germany)
Kuhaudomlarp S
(2018)
Identification of Euglena gracilis ß-1,3-glucan phosphorylase and establishment of a new glycoside hydrolase (GH) family GH149.
in The Journal of biological chemistry
O'Neill EC
(2017)
Cellodextrin phosphorylase from Ruminiclostridium thermocellum: X-ray crystal structure and substrate specificity analysis.
in Carbohydrate research
| Description | Enzymatic methods to prepare novel fluorinated and aminated celluloses |
| Exploitation Route | Biodegradable, pH-responsive gels and films. New ways to prepare heavily fluorinated celluloses, which exists in a new polymorphic form. This has impact on the design and use of polysaccharide materials. |
| Sectors | Agriculture, Food and Drink,Healthcare,Manufacturing, including Industrial Biotechology,Pharmaceuticals and Medical Biotechnology |
| Description | New engagement with Unilever, leading to an iCASE bid jointly between Field (Manchester) and Miller (Keele) in the area of polysaccharide structure-property relationships. |
| First Year Of Impact | 2019 |
| Sector | Agriculture, Food and Drink,Environment,Healthcare,Manufacturing, including Industrial Biotechology,Pharmaceuticals and Medical Biotechnology |
| Title | Enzymatic synthesis of HMO analogies |
| Description | Through detailed kinetic analysis, we have been able to understand how to use cellodextrin phosphorylase to transfer non-natural substrates to generate new to nature human milk oligosaccharide analogues |
| Type Of Material | Biological samples |
| Year Produced | 2020 |
| Provided To Others? | Yes |
| Impact | Further studies ongoing to explore HMO analogue bioactivity. |
| Title | Enzyme to prepare beta-glucans |
| Description | Identification of key enzyme and establishment of SOP for the enzymatic synthesis of immune-active beta-glucans. |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2019 |
| Provided To Others? | Yes |
| Impact | Too early to tell |
| Description | CDs |
| Organisation | Newcastle University |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Evaluation of a glycosynthase for novel cyclodextrin production |
| Collaborator Contribution | Provision of clone of wild type enzyme and active site mutant |
| Impact | Too early to judge |
| Start Year | 2017 |
| Description | MD starch |
| Organisation | University of Manchester |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Experimental evaluation of glycan structure and enzyme sensitivityt |
| Collaborator Contribution | Microsecond molecular dynamics simulation to assess glycan-glycan interactions |
| Impact | Too early |
| Start Year | 2017 |
| Description | Sugar nucleotides |
| Organisation | Keele University |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Enzymatic synthsesis |
| Collaborator Contribution | Chemical synthesis - methods development and target synthesis. |
| Impact | New integrated chemical and enzymatic methods for sugar nucleotide synthesis |
| Start Year | 2020 |