Development of a multipurpose DNA microarray for the identification and active surveillance of bacterial pathogens
Lead Research Organisation:
London Sch of Hygiene and Trop Medicine
Department Name: Infectious and Tropical Diseases
Abstract
Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.
Publications
Stabler RA
(2012)
Macro and micro diversity of Clostridium difficile isolates from diverse sources and geographical locations.
in PloS one
Nguyen NT
(2010)
The sudden dominance of blaCTX-M harbouring plasmids in Shigella spp. Circulating in Southern Vietnam.
in PLoS neglected tropical diseases
Witney AA
(2012)
BµG@Sbase--a microbial gene expression and comparative genomic database.
in Nucleic acids research
Stabler RA
(2008)
Comparative analysis of BI/NAP1/027 hypervirulent strains reveals novel toxin B-encoding gene (tcdB) sequences.
in Journal of medical microbiology
Baker S
(2008)
Fatal wound infection caused by Chromobacterium violaceum in Ho Chi Minh City, Vietnam.
in Journal of clinical microbiology
Ligowska M
(2011)
Effect of chicken meat environment on gene expression of Campylobacter jejuni and its relevance to survival in food.
in International journal of food microbiology
Parkhill J
(2011)
Bacterial epidemiology and biology--lessons from genome sequencing.
in Genome biology
Stabler RA
(2013)
Characterization of water and wildlife strains as a subgroup of Campylobacter jejuni using DNA microarrays.
in Environmental microbiology
Stabler RA
(2008)
Development and application of the active surveillance of pathogens microarray to monitor bacterial gene flux.
in BMC microbiology
| Description | DSTL, Application of ASP array to high category pathogens |
| Amount | £295,000 (GBP) |
| Organisation | Defence Science & Technology Laboratory (DSTL) |
| Sector | Public |
| Country | United Kingdom |
| Start | 03/2007 |
| End | 04/2010 |
| Description | Wellcome Trust Programme Grant (Genetic and phenotypic characterisation of emerging virulent Clostridium difficile) |
| Amount | £577,695 (GBP) |
| Organisation | Wellcome Trust |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 09/2009 |
| End | 05/2015 |
| Title | Data base for ASP array |
| Description | 30,000 validated oligonucleotide sequences on the ASP array on http://www.biomedcentral.com/content/supplementary/1471- 2180-8-177-S1.xls] |
| Type Of Material | Biological samples |
| Year Produced | 2007 |
| Provided To Others? | Yes |
| Impact | Sequences from data base have diagnostic value for highly virulent bacterial and viral pathogens |
| Description | Clostridium difficile strains from ireland outbreaks |
| Organisation | UCB Pharma |
| Country | United Kingdom |
| Sector | Private |
| PI Contribution | Collaborators provide DNA from unique collection of strains and we test on the active surveillance pathogen (ASP) array |
| Collaborator Contribution | Strain provision |
| Impact | Test for hypervirulent Clostridium difficile strains |
| Start Year | 2006 |
| Description | High category pathogens |
| Organisation | Defence Science & Technology Laboratory (DSTL) |
| Country | United Kingdom |
| Sector | Public |
| PI Contribution | Collaborators provide DNA from unique collection of strains and we test on the active surveillance pathogen (ASP) array |
| Collaborator Contribution | Technical know how and strains for testing |
| Impact | Facilitate rapid identification of high category pathogens and strain that have unexpectedly gained DNA |
| Start Year | 2006 |
| Description | Vietnamese strains |
| Organisation | University of Oxford |
| Department | Oxford University Clinical Research Unit Vietnam (OUCRU) |
| Country | Viet Nam |
| Sector | Academic/University |
| PI Contribution | Collaborators provide DNA from unique collection of strains and we test on the active surveillance pathogen (ASP) array |
| Collaborator Contribution | Provision of strains |
| Impact | Test of Shigella, Chromobacterium and several unusual pathogens. Publication and another in preparation |
| Start Year | 2007 |
| Description | Open lecture to general public |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Public/other audiences |
| Results and Impact | Open lecture to general public entitled "Genes, Germs and Genomes" as part of the Royal Irish Academy annual lecture Interest from several newspapers |
| Year(s) Of Engagement Activity | 2009,2011,2012 |
| Description | TV Broadcasts |
| Form Of Engagement Activity | A press release, press conference or response to a media enquiry/interview |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Public/other audiences |
| Results and Impact | BBC Breakfast TV interview on vaccine design and antimicrobial resistance BBC one interview on Campylobacter in Food chain One Show BBC on antimicrobial resistance |
| Year(s) Of Engagement Activity | 2015 |
| Description | lecture to science media |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Media (as a channel to the public) |
| Results and Impact | Lectured to audience of science media on exploitation of genomics Some media interviews |
| Year(s) Of Engagement Activity | 2009 |