Optimal Protocols for Storage of Embryonic and Fetal Neural Stem Cells

Lead Research Organisation: Cardiff University
Department Name: School of Biosciences

Abstract

Involving neural tissue transplantation in the brain, as well as stem cells, human embryos and animal experiments, this research is high profile in the general as well as scientific news media. In addition to publication in peer reviewed journals, the results of the present project will be widely disseminated to the biomedical research community, to NHS and clinical professions, and to interested charities and the lay public, through lectures and seminars in the normal way as well as in media interviews when appropriate. The PIs already have extensive experience in public communication of research results. We strive to achieve
the best balance between the competing demands of proper disclosure of publicly funded science, public
education, promoting enthusiasm for science and support for the MRC and biomedical research, but without raising false or premature hopes for cures that remain distant. The credibility of the team will be markedly enhanced by the present collaboration between three PIs actively involed in neural transplantation and stem cell biology with two PIs who have established expertise in clinical obstetrics and gynaecology, cryobiology and embryological storage protocols.

Technical Summary

This application is for a strategic project grant in response to the cross-research council call in the national strategy to develop stem cell technology. The project is a component in a co-ordinated stem cell initiative being developed in Cardiff. In this project we will determine and characterise the optimal procedures for cryo-storage of expanded fetal neural stem cells, with reference to comparable optimal procedures for embryonic stem cells and primary fetal tissues. The primary outcome criteria will be cell viability, survival and capacity for specific differentiation, to be assessed both in vitro and in vivo by transplantation into adult mouse models of Parkinson?s and Huntington?s diseases. The resulting protocols are of direct relevance for the identification of suitable protocols for storage of alternative stem cell lines in the MRC Stem Cell bank, as well as in our own and others individual research programmes.

Publications

10 25 50
 
Description GTAC
Geographic Reach National 
Policy Influence Type Participation in a national consultation
Impact development of national ethical and regulatory guidelines
 
Description GTAC
Geographic Reach National 
Policy Influence Type Participation in advisory committee
Impact member of GTAC committee reviewing applications and developing policy on stem cell and gene therapy trials in the UK
 
Description MRC
Geographic Reach National 
Policy Influence Type Participation in advisory committee
Impact Targetted direction, policy and funding of UK stem cell research .
 
Description MRC Stem Cells
Geographic Reach National 
Policy Influence Type Participation in advisory committee
Impact I participate in the MRC UK Stem Cell Bank users group and contribute regularly to the policy discussions at head office on stem cell strategy and funding.
 
Description MRC stem cell
Geographic Reach National 
Policy Influence Type Participation in advisory committee
Impact I participate in the MRC Stem Cell Bank User's group and participate regularly in MRC stem cell policy and funding strategy meetings at Head Office.
 
Description MRC stem cells
Geographic Reach National 
Policy Influence Type Participation in advisory committee
Impact I participate in the MRC Stem Cell Bank User's group and participate regularly in MRC stem cell policy and funding strategy meetings at Head Office.
 
Description regular citations in clinical reviews and publications
Geographic Reach Multiple continents/international 
Policy Influence Type Citation in other policy documents
Impact influence on development of NECTAR and ASNTR guidelines
 
Title mTOPs 
Description Validation of alternative protocols for consented collection of human fetal tissue 
Type Of Material Improvements to research infrastructure 
Year Produced 2009 
Provided To Others? Yes  
Impact Resolves the outstanding block on limited tissue availability to allow multiple international research and clinical trials to resume. 
 
Description UKSCB 
Organisation National Institute for Biological Standards and Control (NIBSC)
Department UK Stem Cell Bank
Country United Kingdom 
Sector Charity/Non Profit 
PI Contribution Ongoing partnership with MRC UKSCB in developing protocols for human stem cell handling and processing for clinical grade applications.
Collaborator Contribution Ongoing partnership with MRC UKSCB in developing protocols for human stem cell handling and processing for clinical grade applications.
Impact Ongoing partnership with MRC UKSCB in developing protocols for human stem cell handling and processing for clinical grade applications. No publications yet.
Start Year 2006
 
Description 10-15 presentations to HDA and PDS patient groups 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Primary Audience Participants in your research and patient groups
Results and Impact 10-15 presentations to HDA and PDS patient groups. I present regular medical and lay seminars around the country and internationally. I am afraid I am unable to list all the individual presentations.

academic, medical and patient contacts
Year(s) Of Engagement Activity 2006,2007,2008,2009,2010
 
Description 10-15 presentations to HDA and PDS patient groups. 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Primary Audience Public/other audiences
Results and Impact I present regular medical and lay seminars around the country and internationally. I am afraid I am unable to list all the individual presentations.

various
Year(s) Of Engagement Activity 2006,2007,2008,2009,2010
 
Description 10-15 presentations to HDA and PDS patient groups. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Primary Audience Participants in your research and patient groups
Results and Impact 10-15 presentations to HDA and PDS patient groups. I present regular medical and lay seminars around the country and internationally. I am afraid I am unable to list all the individual presentations.

various
Year(s) Of Engagement Activity 2006,2007,2008,2009,2010
 
Description results included in multiple public and academic lectures and seminars 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact results included in multiple public and academic lectures and seminars

various
Year(s) Of Engagement Activity 2006,2007,2008,2009,2010
 
Description results included in multiple public and academic lectures and seminars 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Primary Audience Public/other audiences
Results and Impact results included in multiple public and academic lectures and seminars

various
Year(s) Of Engagement Activity 2006,2007,2008,2009,2010