Neuropeptide regulation of Adult Neural Stem Cell proliferation

Lead Research Organisation: University of Southampton
Department Name: Clinical Neurological Sciences

Abstract

Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.

Publications

10 25 50
 
Description Brain Repair and Intracranial Neurotherapeutics - the Wales BRAIN Unit
Amount £800,000 (GBP)
Organisation Welsh Assembly 
Sector Public
Country United Kingdom
Start 04/2018 
End 03/2020
 
Description Clinical research training fellowship
Amount £246,000 (GBP)
Organisation Epilepsy Research UK 
Sector Charity/Non Profit
Country United Kingdom
Start 03/2013 
End 02/2016
 
Description DT/E005039/1 EPSRC Stem cell Initiative Chameleon Spots 1
Amount £808,154 (GBP)
Funding ID DT/E005039/1 
Organisation Engineering and Physical Sciences Research Council (EPSRC) 
Sector Public
Country United Kingdom
Start 10/2007 
End 09/2010
 
Description Epilepsy Research UK Project Grant
Amount £60,000 (GBP)
Organisation Epilepsy Research UK 
Sector Charity/Non Profit
Country United Kingdom
Start 10/2006 
End 09/2009
 
Description ISSF funding
Amount £30,000 (GBP)
Organisation Wellcome Trust 
Sector Charity/Non Profit
Country United Kingdom
Start 10/2012 
End 09/2014
 
Description MRC Capacity Building Studentships
Amount £80,000 (GBP)
Organisation Medical Research Council (MRC) 
Sector Public
Country United Kingdom
Start 10/2009 
End 02/2014
 
Description The Hodge Centre for Neuropsychiatric Immunology
Amount £1,021,169 (GBP)
Funding ID Co-applicant 
Organisation The Jane Hodge Foundation 
Sector Charity/Non Profit
Country United Kingdom
Start 02/2017 
End 01/2022
 
Description University of Southampton, Kerkut Trust, PhD Studentship
Amount £60,000 (GBP)
Organisation The Gerald Kerkut Charitable Trust 
Sector Charity/Non Profit
Country United Kingdom
Start 10/2007 
End 09/2010
 
Title Use of Y1 and VPAC2 knock out mice for investigating adult neurogenesis 
Description By identifying the VPAC2 receptor as a key messenger for maintaining the pool of symmetrically dividing stem/progenitor cells in the hippocampus and the NPY Y1 receptor for controlling the proliferation rate of Type 2 precursor cells, we have identified these mice as models for studying the control of neurogenesis. 
Type Of Material Model of mechanisms or symptoms - mammalian in vivo 
Year Produced 2007 
Provided To Others? Yes  
Impact 1. Indentification of a role for the Y1 receptor in seizure induced neurogenesis - See Publications 2. Publications by other groups showing an in-vivo effect of NPY on neurogenesis in the hippocampus and subventricular zones - Agasse F et al Stem Cells. 2008 Jun;26(6):1636-45. Decressac M et al Neurobiol Dis. 2009 Jun;34(3):441-9. 
 
Description Chameleon Spots 
Organisation Capsant Neurotechnologies
Country United Kingdom 
Sector Private 
PI Contribution Significant intellectual input into successfully generating 3D HiSpot tissue cultures of fetal and adult human brain as well as the characterisation of these tissue cultures.
Collaborator Contribution Salary and Direct costs from joint funded DTI EPSRC grant developing 3D cultures of Human Fetal and Adult Neural Stem Progenitor Cells. Access to viral reporter contructs for lineage determination and tracking
Impact Successful generation of 3D cultures of adult human brain tissue has allowed us to examine the mechanisms underlying reduced hippocampal neurogenesis in human temporal lobe epilepsy due to hippocampal sclerosis.
Start Year 2006
 
Description Cytokines and Neurogenesis 
Organisation GlaxoSmithKline (GSK)
Country Global 
Sector Private 
PI Contribution Characterised the effects of cytokines and microglia on hippocampal stem/progenitor cells
Collaborator Contribution PhD Studentship
Impact Publications being prepared for submission Additional experiments completed
 
Description Cytokines mediation of altered neurogenesis in TLE 
Organisation Mario Negri Institute for Pharmacological Research
Department Department of Pharmacology
Country Italy 
Sector Charity/Non Profit 
PI Contribution PI
Collaborator Contribution Provision of RAGE and HMGB1 pharmacology and knockout mice
Impact ERUK Clinical Research Training Fellowship 2013-2016
Start Year 2011
 
Description Effects of NPY on status epilepticus induced neurogenesis 
Organisation University College London
Department Institute of Neurology
Country United Kingdom 
Sector Academic/University 
PI Contribution Experimental design and Quantification of neurogenesis in-vivo
Collaborator Contribution Provision of animal models for ICV injection of NPY after status epilepticus
Impact ongoing
Start Year 2010
 
Description Effects of non-status models on hippocampal neurogenesis 
Organisation University of Birmingham
Department College of Medical and Dental Sciences
Country United Kingdom 
Sector Academic/University 
PI Contribution Experimental design and quantifiaction of neurogenesis
Collaborator Contribution Provisionj of tetanus toxing model for studying the effects of spontaneous seizures on neurogenesis in the hippocampus
Impact Paper in preparation Presentation at UK ILAE Scientific meeting 2010
Start Year 2009
 
Description Fine Pattern Separation in mTLE 
Organisation Cardiff University
Department School of Psychology
Country United Kingdom 
Sector Academic/University 
PI Contribution Providing a pathological disease with altered neurogenesis to see if pattern separation is abnormal in humans
Collaborator Contribution Providing the testing equipment for patient studies Providing operant boxes and expertise for pattern seperation and anxiety studies in Animal Models
Impact ERUK Grant funding Waterloo Foundation Funding
Start Year 2015
 
Description Fine Pattern Separation in mTLE 
Organisation University of Cambridge
Department Department of Psychology
Country United Kingdom 
Sector Academic/University 
PI Contribution Providing a pathological disease with altered neurogenesis to see if pattern separation is abnormal in humans
Collaborator Contribution Providing the testing equipment for patient studies Providing operant boxes and expertise for pattern seperation and anxiety studies in Animal Models
Impact ERUK Grant funding Waterloo Foundation Funding
Start Year 2015
 
Description NPY and Seizure induced Neurogenesis 
Organisation Columbia University
Country United States 
Sector Academic/University 
PI Contribution Lead the collaboration, performed all the immunohistochemistry and quantification.
Collaborator Contribution Provision of NPY -/- mice and performing some experiments on these animalsProvision of NPY Y1 -/- mice and BrdU labeling experiments
Impact 17317195
Start Year 2006
 
Description NPY and Seizure induced Neurogenesis 
Organisation The Garvan Institute for Medical Research
Country Australia 
Sector Hospitals 
PI Contribution Lead the collaboration, performed all the immunohistochemistry and quantification.
Collaborator Contribution Provision of NPY -/- mice and performing some experiments on these animalsProvision of NPY Y1 -/- mice and BrdU labeling experiments
Impact 17317195
Start Year 2006
 
Description Neuro-Immune regulation of neurogenesis 
Organisation University of Southampton
Department School of Medicine Southampton
Country United Kingdom 
Sector Academic/University 
PI Contribution PI
Collaborator Contribution Provision of T helper cells to develop co-culture systems to study the effects of neuropeptides on modulating the immune control of hippocampal neurogenesis
Impact Ongoing PhD studentship
Start Year 2010
 
Description Role of Complement in altered neurogenesis in TLE 
Organisation Cardiff University
Department Department of Medical Biochemistry
Country United Kingdom 
Sector Academic/University 
PI Contribution PI We have provided the hypothesis that Complement plays a significant role in altered neurogenesis in Chronic TLE (temporal lobe epilepsy) and the models for testing this hypothesis in vitro and in vivo
Collaborator Contribution Provision of complement agonists and antagonists and Complement receptor knockout mice for in vivo testing
Impact Sussessful Wellcome Trust ISSF funding to support my current MRC phd Student
Start Year 2012
 
Description VIP and neurogenesis 
Organisation University of Edinburgh
Department Centre for Cardiovascular Science
Country United Kingdom 
Sector Academic/University 
PI Contribution Discovered and described the effects of the neuropeptide VIP on hippocampal Stem/progenitor cells in-vitro via VPAC2 and VPAC1 receptors and then characterised the effects in-vivo in a germloine Vipr2 knockout mouse
Collaborator Contribution Provided access to VPAC2 -/- mice to validate in-vivo effects and performed some collaborative experimentsProvided access to VPAC2 -/- mice to validate in-vivo effects and performed some collaborative experiments
Impact 19650041
Start Year 2008
 
Description VIP and neurogenesis 
Organisation University of Edinburgh
Department Centre for Neuroregeneration (CNR)
Country United Kingdom 
Sector Academic/University 
PI Contribution Discovered and described the effects of the neuropeptide VIP on hippocampal Stem/progenitor cells in-vitro via VPAC2 and VPAC1 receptors and then characterised the effects in-vivo in a germloine Vipr2 knockout mouse
Collaborator Contribution Provided access to VPAC2 -/- mice to validate in-vivo effects and performed some collaborative experimentsProvided access to VPAC2 -/- mice to validate in-vivo effects and performed some collaborative experiments
Impact 19650041
Start Year 2008
 
Title Fluoxetine for restoring learning in Temporal Lobe Epilepsy 
Description Oral Fluoxetine for restoring hippocampal neurogeneis in temporal lobe epilepsy. CTIMP beginning March 2016 Epilepsy Research UK 
Type Therapeutic Intervention - Drug
Current Stage Of Development Early clinical assessment
Year Development Stage Completed 2016
Development Status Under active development/distribution
Impact Discussions on research collaborations ongoing with GSK 
 
Title Fluoxetine restores learning deficits in in-vivo model of temporal lobe epilepsy 
Description Off license use of the antidepressant Fluoxetine for restoring learning in temporal lobe epilepsy 
Type Therapeutic Intervention - Drug
Current Stage Of Development Refinement. Non-clinical
Year Development Stage Completed 2011
Development Status Under active development/distribution
Impact Identifies a family of drugs that can be explored in clinical trials to reverse hippocampus dependant learning deficits 
 
Description Astra Zenica Gothenberg 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Primary Audience Public/other audiences
Results and Impact Presented to neuroscience group of research scientists

ongoing collaboration with AZ spinout company
Year(s) Of Engagement Activity 2007
 
Description SEDA 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? Yes
Primary Audience Public/other audiences
Results and Impact Presented work on neuropeptides and adult neurogenesis to a Biotech audience

Instigated discussions with Pfizer
Year(s) Of Engagement Activity 2008