Voltage control of G-protein-coupled receptor signalling
Lead Research Organisation:
University of Cambridge
Department Name: Physiology
Abstract
Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.
Publications

Gurung IS
(2008)
Novel consequences of voltage-dependence to G-protein-coupled P2Y1 receptors.
in British journal of pharmacology

Mahaut-Smith MP
(2008)
A role for membrane potential in regulating GPCRs?
in Trends in pharmacological sciences

Markovic D
(2012)
FRET-based detection of M1 muscarinic acetylcholine receptor activation by orthosteric and allosteric agonists.
in PloS one

Martinez-Pinna J
(2010)
Direct voltage control of endogenous lysophosphatidic acid G-protein-coupled receptors in Xenopus oocytes.
in The Journal of physiology

Martinez-Pinna J
(2005)
Direct voltage control of signaling via P2Y1 and other Galphaq-coupled receptors.
in The Journal of biological chemistry

Osman S
(2016)
Detachment of surface membrane invagination systems by cationic amphiphilic drugs.
in Scientific reports

Pitt SJ
(2005)
Potentiation of P2Y receptors by physiological elevations of extracellular K+ via a mechanism independent of Ca2+ influx.
in Molecular pharmacology

Rinne A
(2015)
The mode of agonist binding to a G protein-coupled receptor switches the effect that voltage changes have on signaling.
in Science signaling
Description | Dissemination of techniques for the study of platelets and megakaryocytes |
Geographic Reach | Multiple continents/international |
Policy Influence Type | Influenced training of practitioners or researchers |
Description | FRET-based measurements of GPCR activation |
Amount | £71,700 (GBP) |
Organisation | British Heart Foundation (BHF) |
Sector | Charity/Non Profit |
Country | United Kingdom |
Start | 01/2006 |
End | 03/2007 |
Description | University Infrastructure award |
Amount | £16,500 (GBP) |
Organisation | University of Leicester |
Sector | Academic/University |
Country | United Kingdom |
Start | 03/2012 |
End | 04/2013 |
Title | FRET assay of m1 GPCR |
Description | Developed a tagged G-protein-coupled receptor that allows detection of its activation by fluorescence readout. the GPCR tagged has been reported to be voltage-dependent |
Type Of Material | Technology assay or reagent |
Year Produced | 2008 |
Provided To Others? | Yes |
Impact | led to collaborations on studies of GPCR activation |
Title | Methodology for the study of megakaryocytes |
Description | Development of methods to purify primary megakaryocytes (platelet precursor cells) from marrow for screening/database generation etc |
Type Of Material | Technology assay or reagent |
Year Produced | 2012 |
Provided To Others? | Yes |
Impact | Published methodology in a book chapter (PMID 22130713); has been used and referred to by other groups. |
Description | Collaboration on the mechanism of voltage control of G-protein-coupled receptors |
Organisation | Philipp University of Marburg |
Country | Germany |
Sector | Academic/University |
PI Contribution | Ideas and a unique molecular tool for analysing voltage control of receptors. Assistance with writing a manuscript |
Collaborator Contribution | Conducted a series of experiments using the unique tool we provided. Generated a high quality scientific paper (uinder review) that advances our understanding of G-protein-coupled receptor signalling |
Impact | Work has been presentaed at an invited talk given at an international meeting. A scientific paper under review is a potential output |
Start Year | 2012 |
Description | FRET-based studies of GPCR voltage control |
Organisation | Philipp University of Marburg |
Department | Institute of Pharmacology and Clinical Pharmacy |
Country | Germany |
Sector | Academic/University |
PI Contribution | We have developed a novel sensor of muscarinic receptors for use in studies of voltage control of GPCRs |
Collaborator Contribution | The group are experts in FRET measurements of GPCRs |
Impact | On-going research collaboration |
Start Year | 2011 |
Description | Voltage control of GPCRs in oocytes |
Organisation | University of Alicante |
Country | Spain |
Sector | Academic/University |
PI Contribution | provided some personel and ideas/concepts |
Collaborator Contribution | Expertise at collaborating institution has provided ability to conduct work in a heterologous expression system and report the first measurements of voltage-control of a GPCR in such a system (Martinez-Pinna et al 2010) |
Impact | Direct voltage control of endogenous lysophosphatidic acid G-protein-coupled receptors in Xenopus oocytes. Martinez-Pinna J, Gurung IS, Mahaut-Smith MP, Morales A. J Physiol. 2010 May 15;588(Pt 10):1683-93. |
Start Year | 2009 |
Description | Inaugural lecture open to public |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Type Of Presentation | Keynote/Invited Speaker |
Geographic Reach | National |
Primary Audience | Public/other audiences |
Results and Impact | Professorial Inaugural lecture to general audience including public, University staff and academics Part of general awareness of loacl public of research activities of University staff |
Year(s) Of Engagement Activity | 2010 |
Description | International guest lecturer on Masters in Bioscience |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Type Of Presentation | Keynote/Invited Speaker |
Geographic Reach | International |
Primary Audience | Postgraduate students |
Results and Impact | Attended by the Masters students taking the degree in Medical Bioscience at the university of Alicante, along with research students of the Biomedical Departments, university of alicante Stimulated future research and teaching visits between University of Leicester and University of Alicante |
Year(s) Of Engagement Activity | 2011 |
Description | Invited presentation at international conference (Frontiers in Synaptic Function, Jerusalem 11-14 Sept 2014) |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Participants in your research and patient groups |
Results and Impact | Promoted an understanding of the concept of voltage control of G-protein-coupled receptors, which may influence cellular signalling in many cell types and could also influence how pharmacological reagents modify cellular responses Promoted discussion and proposals for additional collaborative experiments that could be conducted in several internationally renowned laboratories |
Year(s) Of Engagement Activity | 2014 |
URL | http://www.as.huji.ac.il/content/frontiers-synaptic-functions |
Description | Masterclass for PhD students |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | Yes |
Geographic Reach | National |
Primary Audience | Public/other audiences |
Results and Impact | Provides an advanced understanding of a key technique used in biological/biomedical research Provided an increased understanding of the technique and generated many questions of its use and potential problems. Also provided an assessment of the students involvement in the class. |
Year(s) Of Engagement Activity | 2014 |
URL | http://www2.warwick.ac.uk/fac/cross_fac/mibtp/about_mibtp/ |