Voltage control of G-protein-coupled receptor signalling

Lead Research Organisation: University of Cambridge
Department Name: Physiology

Abstract

Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.
 
Description Dissemination of techniques for the study of platelets and megakaryocytes
Geographic Reach Multiple continents/international 
Policy Influence Type Influenced training of practitioners or researchers
 
Description FRET-based measurements of GPCR activation
Amount £71,700 (GBP)
Organisation British Heart Foundation (BHF) 
Sector Charity/Non Profit
Country United Kingdom
Start 01/2006 
End 03/2007
 
Description University Infrastructure award
Amount £16,500 (GBP)
Organisation University of Leicester 
Sector Academic/University
Country United Kingdom
Start 03/2012 
End 04/2013
 
Title FRET assay of m1 GPCR 
Description Developed a tagged G-protein-coupled receptor that allows detection of its activation by fluorescence readout. the GPCR tagged has been reported to be voltage-dependent 
Type Of Material Technology assay or reagent 
Year Produced 2008 
Provided To Others? Yes  
Impact led to collaborations on studies of GPCR activation 
 
Title Methodology for the study of megakaryocytes 
Description Development of methods to purify primary megakaryocytes (platelet precursor cells) from marrow for screening/database generation etc 
Type Of Material Technology assay or reagent 
Year Produced 2012 
Provided To Others? Yes  
Impact Published methodology in a book chapter (PMID 22130713); has been used and referred to by other groups. 
 
Description Collaboration on the mechanism of voltage control of G-protein-coupled receptors 
Organisation Philipp University of Marburg
Country Germany 
Sector Academic/University 
PI Contribution Ideas and a unique molecular tool for analysing voltage control of receptors. Assistance with writing a manuscript
Collaborator Contribution Conducted a series of experiments using the unique tool we provided. Generated a high quality scientific paper (uinder review) that advances our understanding of G-protein-coupled receptor signalling
Impact Work has been presentaed at an invited talk given at an international meeting. A scientific paper under review is a potential output
Start Year 2012
 
Description FRET-based studies of GPCR voltage control 
Organisation Philipp University of Marburg
Department Institute of Pharmacology and Clinical Pharmacy
Country Germany 
Sector Academic/University 
PI Contribution We have developed a novel sensor of muscarinic receptors for use in studies of voltage control of GPCRs
Collaborator Contribution The group are experts in FRET measurements of GPCRs
Impact On-going research collaboration
Start Year 2011
 
Description Voltage control of GPCRs in oocytes 
Organisation University of Alicante
Country Spain 
Sector Academic/University 
PI Contribution provided some personel and ideas/concepts
Collaborator Contribution Expertise at collaborating institution has provided ability to conduct work in a heterologous expression system and report the first measurements of voltage-control of a GPCR in such a system (Martinez-Pinna et al 2010)
Impact Direct voltage control of endogenous lysophosphatidic acid G-protein-coupled receptors in Xenopus oocytes. Martinez-Pinna J, Gurung IS, Mahaut-Smith MP, Morales A. J Physiol. 2010 May 15;588(Pt 10):1683-93.
Start Year 2009
 
Description Inaugural lecture open to public 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation Keynote/Invited Speaker
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Professorial Inaugural lecture to general audience including public, University staff and academics

Part of general awareness of loacl public of research activities of University staff
Year(s) Of Engagement Activity 2010
 
Description International guest lecturer on Masters in Bioscience 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation Keynote/Invited Speaker
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Attended by the Masters students taking the degree in Medical Bioscience at the university of Alicante, along with research students of the Biomedical Departments, university of alicante

Stimulated future research and teaching visits between University of Leicester and University of Alicante
Year(s) Of Engagement Activity 2011
 
Description Invited presentation at international conference (Frontiers in Synaptic Function, Jerusalem 11-14 Sept 2014) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Participants in your research and patient groups
Results and Impact Promoted an understanding of the concept of voltage control of G-protein-coupled receptors, which may influence cellular signalling in many cell types and could also influence how pharmacological reagents modify cellular responses

Promoted discussion and proposals for additional collaborative experiments that could be conducted in several internationally renowned laboratories
Year(s) Of Engagement Activity 2014
URL http://www.as.huji.ac.il/content/frontiers-synaptic-functions
 
Description Masterclass for PhD students 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? Yes
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Provides an advanced understanding of a key technique used in biological/biomedical research

Provided an increased understanding of the technique and generated many questions of its use and potential problems. Also provided an assessment of the students involvement in the class.
Year(s) Of Engagement Activity 2014
URL http://www2.warwick.ac.uk/fac/cross_fac/mibtp/about_mibtp/