structural mri for rhesus monkeys

Lead Research Organisation: University of Oxford
Department Name: Experimental Psychology

Abstract

Direct public dissemination of this work is limited by the reluctance of scientists to expose themselves to antivivisectionist actions. However, our work often appears in secondary sources such as television and popular science magazines.

Technical Summary

We will use structural magnetic-resonance imaging (MRI) to scan the brains of macaque monkeys. The scans will enable us to accurately place recording electrodes in cortical and subcortical targets in electrophysiological experiments. In ablation experiments, the scans will enable us to place heat lesions or neurotoxic injections accurately in subcortical targets. This facility will allow us to perform experiments that cannot be performed without the benefit of MRI scanning.
 
Description Frontal cortical mechanisms and interactions during learning and decision making
Amount £2,500,000 (GBP)
Funding ID G0902373 
Organisation Medical Research Council (MRC) 
Sector Academic/University
Country United Kingdom
Start 05/2011 
End 04/2016
 
Description Integrated neural networks of the brain
Amount £4,955,047 (GBP)
Funding ID Strategic Award PI Professor John Duncan 
Organisation Wellcome Trust 
Sector Charity/Non Profit
Country United Kingdom
Start 01/2014 
End 04/2020
 
Description Senior Research Fellowship in Basic Biomedical Sciences
Amount £2,307,000 (GBP)
Organisation Wellcome Trust 
Sector Charity/Non Profit
Country United Kingdom
Start 03/2016 
End 02/2021
 
Title MRI scanning non-human primates 
Description database of MRI scans (structural, functional, diffusion tensor scans) in monkeys including neurologically normal controls and lesion groups to compare. 
Type Of Material Biological samples 
Year Produced 2010 
Provided To Others? Yes  
Impact Several publications using the control and lesion group scans development by MR imaging group at FMRIB of further FSL software packages to perform monkey brain extraction (BET) from the MR images 
 
Title resting state fMRI data bases 
Description macaque resting state functional magnetic resonance imaging data sets 
Type Of Material Biological samples 
Year Produced 2014 
Provided To Others? Yes  
Impact papers published by other research groups: Kumar V, Croxson PL, Simonyan K (2016) Structural Organization of the Laryngeal Motor Cortical Network and Its Implication for Evolution of Speech Production. J Neurosci 36:4170-4181. Mitchell DJ, Bell AH, Buckley MJ, Mitchell AS, Sallet J, Duncan J (2016) A Putative Multiple-Demand System in the Macaque Brain. J Neurosci 36:8574-8585. 
 
Description MRI collaboration to set up monkey scanning 
Organisation John Radcliffe Hospital
Department Centre for Functional MRI of the Brain (fMRI)
Country United Kingdom 
Sector Hospitals 
PI Contribution I hold and manage one of the Home Office project licences to carry out this research I've contributed anaesthesia knowledge and management, I've contributed to the set up of MRI scanning in anaesthetised monkeys I've provided anaesthesia for my own and other researchers animals in experiments, I've provided anaesthesia equipment for use in animals under general anaesthesia, I've learnt to operate the MR scanner for MR imaging of monkeys for my own work and other researchers I've developed MR knowledge for diagnositc/clinical purposes related to postoperative neurosurgery complications for monkeys
Collaborator Contribution Partners have contributed to the overall setup of MR scanning in monkeys. Write sequences and continue to optimise these for experiments. provide Q&A support for the scanner image quality provide advice on data storage and management Assist with ideas for analysis of monkey MR imaging and provide MR physics and MR image analysis courses Provide monkey analysis packages via FSL updates Provide overall ongoing support for the MR imaging work Provide scanner operator training and support provide information and training for safety of scanner use and run annual safety courses
Impact outputs for me include training to be scanner operator learning imaging analysis techniques for monkey imaging data received training on how to design MR imaging experiments for monkeys and humans I've developed expertise in using software packages to help with image analysis including FSL, MATLAB, SPM8 I've collected MR scans of my monkeys brains pre-operation and post-operation to document the structural, functional and connectivity changes in their brains as a consequence of the lesions gained knowledge for MR use in diagnosis of complications related to neurosurgery of my monkeys publications listed in relevant section
Start Year 2009
 
Description MRI collaboration to set up monkey scanning 
Organisation University of Oxford
Department Department of Experimental Psychology
Country United Kingdom 
Sector Academic/University 
PI Contribution I hold and manage one of the Home Office project licences to carry out this research I've contributed anaesthesia knowledge and management, I've contributed to the set up of MRI scanning in anaesthetised monkeys I've provided anaesthesia for my own and other researchers animals in experiments, I've provided anaesthesia equipment for use in animals under general anaesthesia, I've learnt to operate the MR scanner for MR imaging of monkeys for my own work and other researchers I've developed MR knowledge for diagnositc/clinical purposes related to postoperative neurosurgery complications for monkeys
Collaborator Contribution Partners have contributed to the overall setup of MR scanning in monkeys. Write sequences and continue to optimise these for experiments. provide Q&A support for the scanner image quality provide advice on data storage and management Assist with ideas for analysis of monkey MR imaging and provide MR physics and MR image analysis courses Provide monkey analysis packages via FSL updates Provide overall ongoing support for the MR imaging work Provide scanner operator training and support provide information and training for safety of scanner use and run annual safety courses
Impact outputs for me include training to be scanner operator learning imaging analysis techniques for monkey imaging data received training on how to design MR imaging experiments for monkeys and humans I've developed expertise in using software packages to help with image analysis including FSL, MATLAB, SPM8 I've collected MR scans of my monkeys brains pre-operation and post-operation to document the structural, functional and connectivity changes in their brains as a consequence of the lesions gained knowledge for MR use in diagnosis of complications related to neurosurgery of my monkeys publications listed in relevant section
Start Year 2009
 
Description Contributor to Lab Animal Tour 
Form Of Engagement Activity Engagement focused website, blog or social media channel
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact A virtual visit to the Oxford lab animal facilityWelcome to Oxford University's primate research facility.

This facility has been specially designed to accommodate behavioural neuroscience research involving Rhesus Macaque (Macaca mulatta) monkeys.

Macaques are socially and cognitively complex animals, characteristics that make them ideal subjects for our behavioural neuroscience studies. Because of these characteristics it is really important that we provide high standards of care.

Our species-specific environmental enrichment program and reward-based training strategies are key to ensuring the animals are appropriately acclimatised to the staff, the laboratory environment and the research procedures.

During this virtual tour you will have an opportunity to meet the staff and animals, to see the facilities and to learn about some of the studies that we carry out.
Year(s) Of Engagement Activity 2017,2018
URL http://www.labanimaltour.org
 
Description UAR Lab animal tour 
Form Of Engagement Activity Engagement focused website, blog or social media channel
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact contributed to Understanding Animals Research videoing of our labs and primates - this was then turned into a virtual lab tour that is freely available on the internet. http://www.labanimaltour.org/ . We won the award for Public Engagement for our efforts in promoting our research and welfare in December 2017.
Year(s) Of Engagement Activity 2017
URL http://www.labanimaltour.org/
 
Description Understanding Animal Research Lab Tour 
Form Of Engagement Activity Engagement focused website, blog or social media channel
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact Understanding Animal Research lab tours provides an easily accessible web resource that allows members of the general public to visit and navigate through a primate neuroscience research facility
Year(s) Of Engagement Activity 2017
URL http://www.labanimaltour.org/
 
Description interview for national news 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Intereviews in national newspapers and scientific journals relating to ultrasound stimulation experiments (eg http://blog.pnas.org/2019/03/journal-club-low-intensity-focused-ultrasound-shows-promise-as-tool-to-probe-deep-brain-function/)
Year(s) Of Engagement Activity 2019
 
Description interviews for newspapers and radio broadcasts 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Primary Audience Media (as a channel to the public)
Results and Impact The first author of a scientific study, Dr Jerome Sallet, spoke to journalists in the UK and abroad, working on newspapers and on radio, about the results he obtained with the MRI scanner funded by the grant.

Several items appeared in radio news and in newspapers in the UK and abroad.
Year(s) Of Engagement Activity 2011
 
Description non-scientific conference 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Primary Audience Policymakers/politicians
Results and Impact The grant supported the purchase of an MRI scanner. One of the applicants, M Rushworth, has presented work undertaken on this scanner at a Skoll Foundation meeting show-casing scientific research on decision making and social cognition and attempting to social entrepreneurs interested in social influence and change.

Organizer reported that the audience had found the event interesting
Year(s) Of Engagement Activity 2011