Cellular and Viral Immunology Resource at the Wales College of Medicine, Cardiff University

Lead Research Organisation: Cardiff University
Department Name: School of Medicine

Abstract

Research in general is very labour intensive, often requiring large quantities of reagents for use in experiments. Thus, a lot of time and effort is required just for reagent generation, even before any experiments can be carried out. The idea of this proposal is to fund a facility that specializes in large-scale reagent generation (including antibodies, soluble proteins, cell lines and viruses), which can then support the research of multiple projects and collaborations. In so doing, it facilitates all of the studies, allowing more time for the researchers to carry out actual experiments rather than spending inordinate amounts of time generating reagents. In addition, it brings together researchers from different backgrounds with different expertise, allowing interaction and complimentation to enhance the research. Finally, such a facility would provide a bank of resources that can then be used whenever needed in the future. In this way, it will enhance the productivity and progress of immunological research at the Wales College of Medicine, as well as research associated with it nationally and internationally. This research ranges from understanding the basic mechanisms that control inflammation and immunity (to viruses, cancers or self ), to studies investigating arthritis and the way that disease-causing pathogens such as persistent viruses impact on the immune response. The results of this research will be presented at conferences and to wider audiences as it advances.

Technical Summary

This proposal aims to provide salary and consumables for a named technician to continue supporting the research of the lead applicant and the co-applicant through the continued running of a tissue culture resource. The lead applicant, Dr Eddie Wang, holds an MRC CEG to study the function of death receptor 3, while the co-applicant, Dr Awen Gallimore, holds an MRC Senior Fellowship to investigate the role of regulatory T cells in a number of disease models. The grant holders already collaborate on research into melanocyte antigens, influenza and lymphocyte choriomeningitis virus (LCMV). These studies require intensive tissue culture, including: screening and storage of cell lines; purification of soluble proteins; production of titred virus stocks and; generation of hybridomas for antibody production. The grant holders also have collaborations with other groups within an existing MRC-COGG that currently provides essential tissue culture support via a facility run by the named technician. This COGG will expire in April 2006. The Collaboration Grant will extend this support for the full duration of the MRC CEG and Senior Fellowship.

Publications

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Tomasec P (2007) Adenovirus vector delivery stimulates natural killer cell recognition. in The Journal of general virology

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Twohig JP (2010) Age-dependent maintenance of motor control and corticostriatal innervation by death receptor 3. in The Journal of neuroscience : the official journal of the Society for Neuroscience

 
Description ARC PhD Studentship
Amount £80,530 (GBP)
Organisation Versus Arthritis 
Start 10/2009 
End 09/2012
 
Description BBSRC CASE Studentship (GSK)
Amount £97,110 (GBP)
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 10/2009 
End 09/2013
 
Description MRC Project Grant
Amount £534,818 (GBP)
Organisation Medical Research Council (MRC) 
Sector Academic/University
Country United Kingdom
Start 12/2009 
End 05/2013
 
Description PhD Studentship
Amount £53,000 (GBP)
Organisation Cardiff University 
Sector Academic/University
Country United Kingdom
Start 10/2009 
End 09/2012
 
Description Arthritis 
Organisation Cardiff University
Department School of Medicine
Country United Kingdom 
Sector Academic/University 
PI Contribution Reagents and intellectual input
Collaborator Contribution Reagents and intellectual input
Impact Publications - PMID: 18824582 Grants - ARC PhD studentship awarded in 2008
Start Year 2006
 
Description Complement 
Organisation University Hospital of Wales
Department Department of Medical Biochemistry and Immunology
Country United Kingdom 
Sector Hospitals 
PI Contribution Intellectual input
Collaborator Contribution Reagents and intellectual input
Impact Publication - PMID: 16493049
Start Year 2006
 
Description EBV 
Organisation University of Birmingham
Department Birmingham Cancer Research UK Centre
Country United Kingdom 
Sector Academic/University 
PI Contribution Reagents and intellectual input
Collaborator Contribution Reagents and intellectual input
Impact Publication - PMID: 17079298
Start Year 2006
 
Description HCMVA 
Organisation University Hospital of Wales
Department Department of Medical Microbiology
Country United Kingdom 
Sector Hospitals 
PI Contribution Reagents and intellectual input
Collaborator Contribution Reagents and intellectual input
Impact Publications - PMID: 17522202, 17372005, 17374753, 18287244 Grants - MRC project grant awarded in 2007
 
Description HCMVB 
Organisation University Hospital of Wales
Department Department of Medical Microbiology
Country United Kingdom 
Sector Hospitals 
PI Contribution Reagents and intellectual input
Collaborator Contribution Reagents and intellectual input
Impact Publications - PMID: 18383036, 17522202, 17372005, 17374753, 18287244 Grants - MRC project grant awarded in 2007
 
Description MCMV 
Organisation University Hospital of Wales
Department Department of Medical Biochemistry and Immunology
Country United Kingdom 
Sector Hospitals 
PI Contribution Staff, reagents and intellectual input
Collaborator Contribution Reagents and intellectual input
Impact Grants - MRC project grant awarded in 2009
Start Year 2007