Lipids Detected by 1H Magnetic Resonance Spectroscopy as Brain Tumour Biomarkers

Lead Research Organisation: University of Birmingham
Department Name: Neuroscience

Abstract

We use Proton Magnetic Resonance Spectroscopy (MRS) to examine the biochemical nature of lipid species in paediatric brain tumours. Brain tumours are the most common form of solid tumours in the child population. Clinical management of paediatric brain tumour patients would benefit from imaging techniques able to indicate tumour aggressiveness in regard to growth and invasion as well as allowing assessment of treatment response.

Our goal is to find a link between the MRS lipids and clinical behaviour of brain cancer. Preclinical and clinical studies have indicated that saturated lipids by MRS are indicative of the degree of malignancy. Similarly, during successful therapy MRS signals from unsaturated lipids change indicating cell death at an early stage.

The work will be carried out in one of the largest children’s cancer centers in the UK. The MRS results recorded from tumours will be compared with those obtained from tumour tissue collected at operations. Clinical information, including patient outcome and event-free survival, will be compared with MRS lipids and other lipid analyses from tumours.

We expect that the results from the MRS project will allow doctors to predict how aggressive a brain tumour is and how well it will respond to treatment without a biopsy. Repeating MRS during treatment it is expected should provide an efficient means for monitoring treatment response, making it possible to adjust treatment to achieve an optimal outcome without delay.

Technical Summary

1H magnetic resonance spectroscopy (MRS) reveals resonances from CH2/CH3 groups of saturated mobile lipids in brain tumours, but not in the healthy brain parenchyma. Evidence from the applicants and others links the 1H MRS detectable lipids to poor prognosis. Our data from experimental gliomas show that 1H MRS signals from polyunsaturated lipids (PUFA) are excellent non-invasive biomarkers for apoptosis induced by cytotoxic therapy. The proposal is to qualify biomarker value of 1H MRS detected saturated and PUFA lipids. Hypotheses, claiming that (a) saturated 1H MRS lipids are prognostic biomarkers and (b) PUFAs biomarkers for therapy monitoring, will be examined. The project involves both retrospective (80 patients) and prospective (~35 patients annually) paediatric brain tumour cohorts of Birmingham Children Hospital (BCH) as clinical patient population. Paediatic patients undergo regular 1H MRS during the follow-up at BCH, extending to six years for a subset of cases, and tissue specimens are obtained from about 80% of tumours. State-of-the-art in vivo MRS technology, data processing and lipid analyses, including MALDI-MS technique on histological specimens, will be used. The project involves two commercial partners (Waters, MA, USA and MRSTools Inc., Finland). The 1H MRS non-invasive biomarkers are expected to improve patient management, reduce need for invasive diagnostic procedures and enable efficient objective therapy monitoring. In long term, specific molecular biomarkers may be linked to targeted new therapeutic agents.

Publications

10 25 50

 
Description CCLG CNS Division and Clinical Studies Group
Geographic Reach Multiple continents/international 
Policy Influence Type Participation in advisory committee
Impact Dr Peet is a member of the Central Nervous System Tumour Division of the CCLG and now the NCRI Clinical Studies subgroup. One role of this group is the construction of national protocols for radiology use in clinical trials. Dr Peet contributed to and is named on the UK guidelines for High Grade Glioma management, he was responsible for the functional imaging section.
 
Guideline Title Radiology Guidelines
Description CCLG Radiology Guidelines
Geographic Reach North America 
Policy Influence Type Citation in clinical guidelines
Impact Standardisation of radiology protocols and a systematic strategy for evaluating new techniques and including them within the clinical practice is essential for translation of the new technology into routine clinicial practice.
 
Description Citation in Lancet Neurology
Geographic Reach Multiple continents/international 
Policy Influence Type Citation in clinical reviews
URL http://europepmc.org/abstract/MED/18031705
 
Description Clinical decision support systems for magnetic resonance spectroscopy in brain tumous
Geographic Reach Asia 
Policy Influence Type Influenced training of practitioners or researchers
 
Description Leader Article for Archives of Diseases in Childhood
Geographic Reach Multiple continents/international 
Policy Influence Type Citation in clinical reviews
URL http://europepmc.org/abstract/MED/18463122
 
Description Magnetic Resonance Spectroscopy named as being being important Safe and Sustainable neurosurgery for children
Geographic Reach National 
Policy Influence Type Participation in advisory committee
 
Description SIOPE Brain Imaging Group Co-Chair
Geographic Reach Europe 
Policy Influence Type Membership of a guidance committee
Impact Formation of imaging guidelines across Europe for children with brain tumours, clinical trials led by SIOPE have improved survival, decreased morbidity and improved quality of life
 
Description A Coordinated Infrastructure for NMR in the Physical and Life Sciences: A 1 GHz Spectrometer at Birmingham
Amount £6,558,752 (GBP)
Funding ID EP/R030030/1 
Organisation Engineering and Physical Sciences Research Council (EPSRC) 
Sector Academic/University
Country United Kingdom
Start 05/2018 
End 04/2021
 
Description BCH RF - PhD Studentship- Novel metabolite biomarkers of prognosis in childhood tumours
Amount £68,276 (GBP)
Funding ID BCHRF287 
Organisation BCH Charities 
Sector Charity/Non Profit
Country United Kingdom
Start 10/2012 
End 09/2015
 
Description BCH RF - Project Grant - 31P Magnetic Resonance Spectroscopy as an early biomarker of tumour response in childhood tumours.
Amount £69,737 (GBP)
Organisation BCH Charities 
Sector Charity/Non Profit
Country United Kingdom
Start 10/2010 
End 10/2011
 
Description BCH RF - Project Grant - Molecular Characterization of Retinoblastoma
Amount £50,000 (GBP)
Organisation BCH Charities 
Sector Charity/Non Profit
Country United Kingdom
Start 10/2009 
End 10/2010
 
Description BCH RF - Project Grant -Non-invasive investigation of brain metabolism and function in children with neurodegenerative diseases.
Amount £50,000 (GBP)
Organisation BCH Charities 
Sector Charity/Non Profit
Country United Kingdom
Start 10/2009 
End 10/2010
 
Description BCH RF - Springboard Fellowship -Metabolomic approach to clinical evaluation of patients with known and suspected childhood neurodegenerative disorders
Amount £50,000 (GBP)
Organisation BCH Charities 
Sector Charity/Non Profit
Country United Kingdom
Start 10/2009 
End 10/2010
 
Description BCH RF- Project Grant - Probing the tissue microenvironment of tumours by Magnetic Resonance Imaging
Amount £64,082 (GBP)
Organisation BCH Charities 
Sector Charity/Non Profit
Country United Kingdom
Start 10/2011 
End 10/2012
 
Description CRUK Cancer Imaging Programme
Amount £2,300,000 (GBP)
Funding ID C7809/A10342 
Organisation Cancer Research UK 
Sector Charity/Non Profit
Country United Kingdom
Start 11/2008 
End 11/2014
 
Description Childhood Eye Cancer Trust - Project Grant - Identification of molecular tools to guide treatment decisions in retinoblastoma
Amount £51,884 (GBP)
Organisation Childhood Eye Cancer Trust (CHECT) 
Sector Charity/Non Profit
Country United Kingdom
Start 10/2009 
End 10/2012
 
Description ECMC Paediatric Network.
Amount £2,528,618 (GBP)
Funding ID C8232/A25261 
Organisation Cancer Research UK 
Sector Charity/Non Profit
Country United Kingdom
Start 04/2017 
End 03/2022
 
Description ENCCA European Network for Cancer Research in Children and Adolescents (12m euros across 33 participants)
Amount € 13,000,000 (EUR)
Funding ID 261474 
Organisation European Commission 
Sector Public
Country European Union (EU)
Start 01/2011 
End 05/2015
 
Description EPSRC Doctoral Training Centre (Life Sciences Interface) shared with E Claridge
Amount £2,710,000 (GBP)
Organisation Engineering and Physical Sciences Research Council (EPSRC) 
Sector Academic/University
Country United Kingdom
Start 10/2008 
End 10/2014
 
Description Equipment Grant - HWB-NMR a National Resource for biomolecular research,
Amount £496,000 (GBP)
Funding ID 083796 
Organisation Wellcome Trust 
Sector Charity/Non Profit
Country United Kingdom
Start 03/2008 
End 03/2011
 
Description HWB-NMR: a national resource for biomolecular research
Amount £1,487,450 (GBP)
Funding ID 208400/Z/17/Z 
Organisation Wellcome Trust 
Sector Charity/Non Profit
Country United Kingdom
Start 09/2017 
End 09/2022
 
Description Healthcare Scientist Research Fellowship
Amount £114,553 (GBP)
Organisation National Institute for Health Research 
Sector Public
Country United Kingdom
Start 03/2009 
End 03/2013
 
Description Imagine for Margo Project Grant
Amount £192,000 (GBP)
Organisation Imagine For Margo 
Sector Charity/Non Profit
Country France
Start 08/2014 
End 03/2018
 
Description Leukaemia Research Project Grant
Amount £200,000 (GBP)
Organisation Leukaemia and Lymphoma Research 
Sector Charity/Non Profit
Country United Kingdom
Start 10/2007 
End 10/2010
 
Description Little Princess Trust Project Grant - Improving the diagnosis of children's brain tumours by Functional Radiomics
Amount £99,027 (GBP)
Organisation Children's Cancer and Leukaemia Group (CCLG) 
Sector Charity/Non Profit
Country United Kingdom
Start 05/2018 
End 04/2020
 
Description Medical Research Council - Health Data Research UK Substantive Site
Amount £11,502,198 (GBP)
Organisation Medical Research Council (MRC) 
Sector Academic/University
Country United Kingdom
Start 02/2018 
End 01/2023
 
Description Medical Research Council DPFS
Amount £1,980,000 (GBP)
Funding ID MR/P007732/1 
Organisation Medical Research Council (MRC) 
Sector Academic/University
Country United Kingdom
Start 03/2017 
End 02/2022
 
Description NIHR Capital Awards Scheme - 3T MR Research Centre
Amount £3,700,000 (GBP)
Organisation National Institute for Health Research 
Sector Public
Country United Kingdom
Start 09/2009 
End 09/2014
 
Description Project Grant - Developing and Testing a Clinical Decision Support System for Advanced Magnetic Resonance Imaging in Cancer
Amount £45,738 (GBP)
Funding ID BCHRF 513 
Organisation BCH Charities 
Department Birmingham Children's Hospital Research Foundation
Sector Charity/Non Profit
Country United Kingdom
Start 05/2018 
End 04/2019
 
Description Project Grant - Neuroanatomical correlates of cognitive dysfunction in short and long term survivors of childhood medulloblastoma
Amount £298,640 (GBP)
Funding ID GN-000364 
Organisation The Brain Tumour Charity 
Sector Charity/Non Profit
Country United Kingdom
Start 02/2018 
End 03/2021
 
Description Project Grant - Understanding the chemistry of fat droplets in brain cancer cells
Amount £25,000 (GBP)
Organisation Andrew McCartney Trust Fund 
Sector Charity/Non Profit
Country United Kingdom
Start 11/2012 
End 10/2013
 
Description Technology Platform Diagnostic Imaging
Amount £342,000 (GBP)
Organisation National Institute for Health Research 
Sector Public
Country United Kingdom
Start 10/2006 
End 10/2009
 
Title Assessment of MRS quality using small variations in basis functions 
Description Assessing quality of MRS data is essential for its reliable use but poses many challenges. MRS quality is usually assessed by signal to noise ratio, spectral resolution and the fits spectral features. Small variations in simulated individual metabolites show instabilities in the fitting not revealed by the conventional parameters when the data quality is poor. 
Type Of Material Technology assay or reagent 
Year Produced 2010 
Provided To Others? Yes  
Impact Improved automated quality control of MRS data. The method has been published in NMR in Biomedicine. 
 
Title Development of database and online classification 
Description A web accessible database containing clinical information has been extended to include functional imaging data. This database forms the basis for the functional imaging group of the CCLG. Computer programs have been written to perform automated analysis and storage of SVS data. Classification can now be performed for a given case on the database at the request of a user. The most recent version of the database can be accessed at any computer. 
Type Of Material Improvements to research infrastructure 
Year Produced 2008 
Provided To Others? Yes  
Impact The development of the database has been essential for the dissemination work through the specific publications (pubmed number) 17506115, 18613254, 19067434, 19431141 and 19795380. DOI10.1016/j.ejca.2012.09.002 
 
Title TARQUIN fitting tool and metabolite simulation functionality 
Description Spectroscopy can provide highly reproducible quantitative information on variety of molecules, however manual analysis can be labour intensive and user dependant. An analysis tool has been developed which can be applied to both 1H HR-MAS and in-vivo 1H MRS facilitating the quantitative spectroscopic analysis of a mouse brain tumour model. A manuscript detailing the algorithm has been published PMID 20878762. 
Type Of Material Technology assay or reagent 
Year Produced 2008 
Provided To Others? Yes  
Impact Metabolite simulation functionality has been used in PMID 20331867 and the full fitting routine was required for data analysis presented in PMID 20842126. The software is also used routinely for the analysis of adult brain tumour and rodent tumour models in the Kauppinen research group. The software has been downloaded more than 700 times from 46 different countries since Feb 2010. The software is being prepared for commercialisation by a local SME of which a previous PhD student is a director. 
URL http://www.tarquin.sourceforge.net
 
Title bSMOTE for improved diagnostic accuracy using MRS when classes are unbalanced 
Description We have previously developed diagnostic aids for children's brain tumours based on pattern recognition of magnetic resonance spectroscopy metabolite profiles. These of limited accuracy when the library of tumour cases has groups with greatly varying numbers in them which is commonly the case. The borderline synthetic minority oversampling method overcomes this problem by synthesising extra cases to make the groups have similar numbers in them. We have shown that this improves the diagnostic accuracy for rare tumours. 
Type Of Material Model of mechanisms or symptoms - human 
Year Produced 2016 
Provided To Others? Yes  
Impact Rare tumours can be diagnosed more accurately using magnetic resonance spectroscopy. 
 
Title Functional Imaging Database for Birmingham Cancer Clincial Trials Unit 
Description We have been developing a database for functional imaging data for the Cancer CTU in Birmingham for a multi-national clinical trial (BEACON). This is primarily funded by a small charity Imagine for Margo and is being achieved in collaboration with the University of Warwick. 
Type Of Material Database/Collection of data 
Year Produced 2017 
Provided To Others? Yes  
Impact The database is not yet fully in use but will be over the next few months. 
 
Title Library of cases to support the MIROR Clinicial Decision Support System 
Description MIROR is a prototype clinical decision support system which aids the diagnosis of children's cancer from their advanced imaging and spectroscopy data. The DSS contains a library of cases both brain and body tumours against which the index case is compared. 
Type Of Material Data handling & control 
Year Produced 2018 
Provided To Others? Yes  
Impact Paper is accepted for publication and will appear later in the year. We have shown that the DSS has the capability to improve non-invasive diagnosis compared with conventional radiological reporting of standard imaging. 
 
Description CCLG Functional Imaging Group 
Organisation Children's Cancer and Leukaemia Group (CCLG)
Country United Kingdom 
Sector Charity/Non Profit 
PI Contribution The CCLG's functional imaging group develops and evaluates functional imaging for the diagnosis, mangement and understanding of childhood cancer. It does this by translating innovations and pilot data from single centres of excellence to a multicentre clinical setting. We have provided the CCLG with pilot data and techniques which have allowed this translation. Mulitcentre studies have been presented at international conferences and are nearing readiness for journal publication.
Collaborator Contribution The CCLG provides a network of centres with clinical and clinical trials expertise in childhood cancer, it is the UKs network of these centres. The CCLG therefore provides the ideal partner for the translation of discoveries from single centre projects to a multicentre clinical environment. The CCLG has a functional imaging group which has clincians and scientists with expertise in this field further enhancing the translation of discoveries in this field to the multicentre network. Dr Peet is the chair of this group.
Impact 17349783, 17823793, 18613254, 18835152, Multicentre projects have been presented at the International Society of Magnetic Resonance in Medicine, British Society of Neuroradiology and Interational Symposium of Pediatric Neuro-oncology. The CCLG has been awarded a £2.3Million programme grant from Cancer Research UK, EPSRC,MRC and DOH (England) which in part resulted from the input from this MRC biomarkers project.
Start Year 2007
 
Description Health Agents Consortium 
Organisation University of Southampton
Department School of Electronics and Computer Science Southampton
Country United Kingdom 
Sector Academic/University 
PI Contribution We supplied MRS data on childhood brain tumours, helped to design and build the decision support system and in particular a graphical user interface for clinicians and tested the interface on clinicians.
Collaborator Contribution The Health Agents Consortium was formed for an EU Framework 6 STREP to design and build a distributed database and data warehouse for MRS data for brain tumour diagnosis and prognosis. It enabled clinical decision support based on our MRS discoveries to be made available to a larger number of clinical centres.
Impact Four journal publications (no PMIDs since they are Engineering Journals), the decision support system has undergone preparation for CE marking.
Start Year 2006
 
Description Joint International Imaging Lab 
Organisation Nanjing Children's Hospital
PI Contribution After a collaboration with Yu Sun from Nanjing China a formal agreement has been set up between the University of Birmingham, Southeast University China and Nanjing Children's Hospital to promote research
Collaborator Contribution Data and expertise will be shared between the partners
Impact Data sharing between Nanjing Children's Hospital and University of Birmingham
Start Year 2018
 
Description Joint International Imaging Lab 
Organisation Southeast University China
Country China 
Sector Academic/University 
PI Contribution After a collaboration with Yu Sun from Nanjing China a formal agreement has been set up between the University of Birmingham, Southeast University China and Nanjing Children's Hospital to promote research
Collaborator Contribution Data and expertise will be shared between the partners
Impact Data sharing between Nanjing Children's Hospital and University of Birmingham
Start Year 2018
 
Description Joint International Imaging Lab 
Organisation University of Birmingham
Country United Kingdom 
Sector Academic/University 
PI Contribution After a collaboration with Yu Sun from Nanjing China a formal agreement has been set up between the University of Birmingham, Southeast University China and Nanjing Children's Hospital to promote research
Collaborator Contribution Data and expertise will be shared between the partners
Impact Data sharing between Nanjing Children's Hospital and University of Birmingham
Start Year 2018
 
Description MicroArt 
Organisation Micro Art
Country Spain 
Sector Private 
PI Contribution We have supplied clinical, imaging and laboratory data as well as clinical expertise and knowledge.
Collaborator Contribution MicroArt is a spin off company from the University Autonomia de Barcelona. They have expertise in software engineering and in particular the databases and clinical decision support systems. They provide an SME which can help commercialise products.
Impact There have been 2 journal paper publications which have featured MicroArt members. There have been moves towards CE marking of products. Health Agents is listed as a collaborator on the ENCCA FP7 NOE.
 
Description Midlands Neuroimaging Collaboration 
Organisation Aston University
Country United Kingdom 
Sector Academic/University 
PI Contribution We have played a key role in forming a collaboration between the major centres in the midlands involved in clinical neuroimaging.
Collaborator Contribution Setting up a collaboration which has so far had two meetings and a planned symposium.
Impact No outcomes yet but multi-disciplinary - oncology, neurology, radiology, psychology, physics, computing,
Start Year 2017
 
Description Midlands Neuroimaging Collaboration 
Organisation University of Nottingham
Country United Kingdom 
Sector Academic/University 
PI Contribution We have played a key role in forming a collaboration between the major centres in the midlands involved in clinical neuroimaging.
Collaborator Contribution Setting up a collaboration which has so far had two meetings and a planned symposium.
Impact No outcomes yet but multi-disciplinary - oncology, neurology, radiology, psychology, physics, computing,
Start Year 2017
 
Description Midlands Neuroimaging Collaboration 
Organisation University of Warwick
Country United Kingdom 
Sector Academic/University 
PI Contribution We have played a key role in forming a collaboration between the major centres in the midlands involved in clinical neuroimaging.
Collaborator Contribution Setting up a collaboration which has so far had two meetings and a planned symposium.
Impact No outcomes yet but multi-disciplinary - oncology, neurology, radiology, psychology, physics, computing,
Start Year 2017
 
Description PukkaJ PACS Development 
Organisation Pukkaj Ltd
PI Contribution Knowledge of MRI data types
Collaborator Contribution Updating software to take account of different datatypes and making new software available.
Impact The PukkaJ PACS system is a key element to the majority of our clinical MRI/MRS publications
Start Year 2013
 
Description SIOP Europe 
Organisation European Society of Paediatric Oncology (SIOPE)
Country Belgium 
Sector Charity/Non Profit 
PI Contribution We provide expertise in functional imaging, including protocols for data collection, transfer and storage as well as analysis together with pilot data.
Collaborator Contribution SIOPE (the international society for paediatric oncology) is a Europe wide consortium of centres for clinical trials in children with cancer. A recent grant application (12 million euros) has been submitted for an EU network of excellence which includes a workpackage on functional imaging led by Dr A Peet. The network provides a comprehensive set of clinical centres for the dissemination of our findings across Europe.
Impact A grant application has been awarded for an EU FP7 Network of Excellence (£12 Million). A Peet leads the task on Functional Imaging.
Start Year 2009
 
Description SIOPE- Brain Imaging Group 
Organisation European Society of Paediatric Oncology (SIOPE)
Country Belgium 
Sector Charity/Non Profit 
PI Contribution Dr Peet is the co-chair of the group and leads the imaging research activities.
Collaborator Contribution We have established a Brain Imaging Group as part of SIOP Europe and Dr Peet Co-Chairs this group. It has a dual function of radiological review for clinical trials and imaging research. Since SIOPE is the main body for designing and running clinical trials in children with cancer, the groups forms an ideal vehicle for translating advances in imaging research into clinical trials as a prelude to their inclusion in rountine clinical practice.
Impact We have developed an international multi-modal MR imaging protocol for inlcusion in clinical trials.
Start Year 2011
 
Description Waters Corporation, MALDI-MS 
Organisation Waters Corporation
Country United States 
Sector Public 
PI Contribution We supply samples from childhood brain tumours, animals and cell lines as well as relevant clinical and biological information.
Collaborator Contribution Dr Shockor has been advising on the use of MALDI-Mass Spectrometry for sample analysis and has secured equipment time.
Impact Samples have been collected, stored and prepared and are awaiting analysis. A preliminary set of results were presented at the 2009 Keystone meeting on lipid metabolism.
Start Year 2007
 
Description Yu Sun, Nanjing, China 
Organisation Nanjing University (NJU)
Country China 
Sector Academic/University 
PI Contribution Dr Yu Sun was a post doctoral research fellow in my group working on an EU project (STREP HealthAgents). Her background was in computer engineering with expertise in image and signal processing. After the fellowship, she returned to her home city of Nanjing in China and set up a group similar to mine at North Western University, collaborating closely with clinical colleagues in the adult and children's hospitals there. She how has a post where she spends time in China and time in Birmingham and we are setting up a close collaboration with an intend that team members spend time in both centres. We bring expertise in translating imaging research into advanced in clinical practice..
Collaborator Contribution Yu Sun's group brings expertise in computer engineering and in image and signal processing to the collaboration. The large patient base in Nanjing also brings extra translationa potential to the collaboration.
Impact We have recently had a paper accepted for publication which included both groups. We have applied for a travel and collaboration fellowship to the Royal Society with a final outcome expected in April 2017, the award has already been granted by China.
Start Year 2016
 
Title MRS Lipds and metabolites as biomarkers of prognosis in childhood brain tumours 
Description MRS lipids at presentation as a biomarker of survival in children with brain tumours. Initial single centre retrospective analysis (in 2007) provided the hypothesis for this biomarker study. We have been collecting single centre data prospectively to test the validity of the biomarker and this has been published in 2013. Other metabolites of prognostic value have been identified and published. Multi-centre is also being collected in readiness to test the biomarker more robustly. 
Type Diagnostic Tool - Imaging
Current Stage Of Development Early clinical assessment
Year Development Stage Completed 2010
Development Status Actively seeking support
Impact Ability to identify agressive tumours which may not appear so on conventional histopathology or which are not biopsied. 
 
Title MRS profiles for non-invasive diagnosis of childhood brain tumours 
Description MRS protocol for data collection, methodology for analysing the data and provision of a non-invasive diagnostic aid based on the data. The decision support system was developed at a single centre, tested in a multi-national study and has now been shown to give added accuracy compared with the standard method of radiological reporting. 
Type Diagnostic Tool - Imaging
Current Stage Of Development Early clinical assessment
Year Development Stage Completed 2009
Development Status Under active development/distribution
Impact Non-invasive diagnostic aid for children with brain tumours. This has been developed on retrospective single centre data and tested prospectively on a multi-centre basis as well as demonstrating added accuracy to conventional methods. 
 
Title Magic Angle Spinning NMR for rapid diagnosis on childhood brain tumour tissue 
Description A method based on metabolic profiles determined by Magic Angle Spinning high resolution NMR of intact tumour tissue for rapid diagnosis of childhood brain tumours. The method needs on 5mg of tissue and is non-destructive allowing the tissue to be used for other purposes eg validation afterwards. The technique and method for automated analysis has been developed. The technique is being developed via an NIHR grant. 
Type Diagnostic Tool - Imaging
Current Stage Of Development Refinement. Clinical
Year Development Stage Completed 2009
Development Status Under active development/distribution
Impact The technique has been publised PMID19208232 and is undergoing further development and testing in a clinical arena. 
 
Title TARQUIN 
Description A software analysis tool for analysing in vivo magnetic resonance spectroscopy data, which is being developed into a commercial product by an SME through funding stream at the University of Birmingham. CE marking is planned for 2015 
Type Of Technology Software 
Year Produced 2009 
Open Source License? Yes  
Impact Impacts will be established after dissemination 
URL http://www.tarquin.sourcefourge.net
 
Description 2nd Metabolomics Sardinian Scientific School 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact 2nd Metabolomics Sardinian Scientific School was aimed at post-grad students new to the field of metabolomics. We gave seminars and workshops in various tools and techniques in metabolomics.
Year(s) Of Engagement Activity 2016
 
Description Article - Optimistic 
Form Of Engagement Activity A magazine, newsletter or online publication
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact Dr Peet wrote an invited article for a book "what are you optiistic about" written by scientists for the lay audience on 2non-invasive techniques for diagnosing and managing brain tumours2.

The book is yet to be published but will give access to a broad range of the general public for dissemination of our work.
Year(s) Of Engagement Activity 2009
 
Description Articles for Contact Magazine 
Form Of Engagement Activity A magazine, newsletter or online publication
Part Of Official Scheme? Yes
Geographic Reach National
Primary Audience Patients, carers and/or patient groups
Results and Impact Dr A Peet wrote an article on New Horizons in Imaging in Children's Cancer, March Issue 2009 and then an Article on The role of imaging in diagnosing childhood tumours in August 2015

Dissemination of information to the general public and patient groups as well as encouraging these groups to put forward their views concerning the research and ideas for future research
Year(s) Of Engagement Activity 2009,2015
 
Description Brain Tumour Research Showcase 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Professional Practitioners
Results and Impact Presentations and lab tours given to a group of patients and families of those with brain tumours.
Year(s) Of Engagement Activity 2016,2017
 
Description British Neuro-Oncology Society Council 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Governing body of the British Neuro-Oncology Society, producing guidelines, reviewing and commenting on NICE guidance, arranging conferences.
Year(s) Of Engagement Activity 2018
 
Description CCLG CNS Division Newsletter 
Form Of Engagement Activity A magazine, newsletter or online publication
Part Of Official Scheme? Yes
Geographic Reach National
Primary Audience Health professionals
Results and Impact The Central Nervous System Division of the CCLG produces quarterly newsletters for clinicians concerning its acitivities. Dr Peet wrote an article on functional imaging for the newsletter.

Dissemination of research to clinicians and encouragement to take part.
Year(s) Of Engagement Activity 2008
 
Description CNS GCT Meeting Ohio 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact International meeting of experts in treating and researching children and young people with germ cell tumours of the central nervous system. Personally gave key note lecture and participated in debate and future strategy
Year(s) Of Engagement Activity 2017
 
Description Chair Pediatric Study Group ISMRM Hawaii 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Meeting of the Pediatric Study Group of the International Society of Magnetic Resonance in Medicine, plenary presentation, presentations by post graduate students and a discussion on strategy.
Year(s) Of Engagement Activity 2017
 
Description Children's Cancer and Leukaemia Group Executive 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact The governing body of the CCLG, which is the multi-disciplinary professional body for those who treat children with cancer. The executive meets about 5 times a year and has a teleconference about 5 times a year. There is a two day annual general meeting which is attended by about 200 people.
Year(s) Of Engagement Activity 2016,2017,2018
 
Description Childrens Cancer and Leukaemia Group - Research Advisory Group Chair 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact First chair of the research advisory group which successfully set up a research strategy and applied for AMRC membership. To 2018 we have awarded around £2M of grants.
Year(s) Of Engagement Activity 2016,2017,2018
 
Description Co-Chair SIOPE Brain Imaging Group Prague 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Meeting of those involved in designing and running clinical trials of children with brain tumours in Europe, with a specific focus on imaging.
Year(s) Of Engagement Activity 2017
 
Description Conference Presentation - European Society of Paediatric Radiology 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? Yes
Geographic Reach International
Primary Audience Health professionals
Results and Impact Oral Presentation on MRS of pineal tumours

Dissemination of information to paediatric radiologits
Year(s) Of Engagement Activity 2008
 
Description Conference Presentation - International Symposium for Pediatric Neuro-Oncology 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? Yes
Geographic Reach International
Primary Audience Health professionals
Results and Impact 2008 - Chicago, USA - Three oral presentations given on Magnetic Resonance Spectroscopy and Magic Angle Spinning NMR of childhood brain tumours as diagnostic and prognostic aids.
2010 Vienna, Austria - 1 oral and 8 poster presentations
2012 Toronto, Canada - 5 poster presentations
2014 Singapore - 4 poster presentations

This is the main clinical pediatric neuro-oncology conference internationally. It occurs every other year. Dissemination to an audience of key clinicians - oncologists, surgeons, radiologists, radiotherapists and allied health professionals.
Year(s) Of Engagement Activity 2008,2010,2012,2014
 
Description Conference Presentation - International Symposium on Pediatric Neuro-Oncology 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? Yes
Primary Audience Health professionals
Results and Impact Poster presentations based on our latest findings on spectroscopy of the smo mouse model (PMID 20842126) and our study on lipids as a biomarker of survival (manuscript in preparation).

Dissemination of knowledge to clinicians in the field.
Year(s) Of Engagement Activity 2010
 
Description Conference Presentation - National Cancer Research Institute 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? Yes
Geographic Reach National
Primary Audience Health professionals
Results and Impact 2009 Birmingham Poster Presentation by group member on glycine as a prgnostic biomarker, shortlisted for the McElwain Prize.
2010 Liverpool Poster
2011 Liverpool Poster
2014 Liverpool poster

Dissemination to clinicians treating patients with cancer.
Year(s) Of Engagement Activity 2008,2009,2010,2011
 
Description Conference Presentations - British Society of Neuroradiology 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? Yes
Geographic Reach National
Primary Audience Health professionals
Results and Impact 2009 Nottingham - Two oral presentations on MRS in childhood brain tumours - one on glycine as a prognostic biomarker and another on mulit-centre data.
2014 Belfast - one oral presentation showing added value in diagnostic accuracy for an MRS based clinical decision support system compared with conventional radiological review for childrens brain tumours.

Dissemination to a key group of clinicians
Year(s) Of Engagement Activity 2009,2014
 
Description Conference Presentations - International Society of Magnetic Resonance in Medicine 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? Yes
Geographic Reach International
Primary Audience Health professionals
Results and Impact 2009 Hawaii, USA - Oral presentation, 2 oral presentations and 8 posters
2010 Stockholm,Sweden - 2 posters
2011 Montreal, Canada -2 oral presentations, 4 posters
2013 Salt Lake City - 1 oral presentation, 6 posters
2014 Milan, Italy - 2 oral presentations, 6 posters

Dissemination of knowledge to clinicians and researchers in the field - neuroradiologists, MRI researchers
Year(s) Of Engagement Activity 2007,2009,2010,2011,2013
 
Description Conference presentation - European Society for Magnetic Resonance in Medicine and Biology 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? Yes
Geographic Reach International
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact Oral presentation on MRS lipids in brain tumours and an e-poster in the ESMRMB conference

Dissemination of outputs to clinicians in the field
Year(s) Of Engagement Activity 2008
 
Description HHHO Ball and Interview 
Form Of Engagement Activity A broadcast e.g. TV/radio/film/podcast (other than news/press)
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Media (as a channel to the public)
Results and Impact Help Harry Help Others Charity raises funds to support families and patients with Brain Tumours and research, I attended the annual Ball and gave an interview which had wide coverage through media channels such as facebook, I have also given short presentation to the attendees.
Year(s) Of Engagement Activity 2016,2017
 
Description Ideas Lab and Maverick TV 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Dr Peet worked with Ideas Lab at the University of Birmingham who engaged with TV production teams notably Maverick TV who specialise in medical and scientific documentries. A video interview was filmed.

The research was highlighted to TV production teams and may lead a television documentary.
Year(s) Of Engagement Activity 2008,2009
 
Description Inaugural Lecture 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Lecture to celebrate the professorship widely attended by different groups of people, researchers, medical practitioners, patients, families, fundraisers etc. There were many lovely compliments made afterwards.
Year(s) Of Engagement Activity 2018
 
Description Invited conference presentations - European Cancer Conference 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? Yes
Geographic Reach International
Primary Audience Health professionals
Results and Impact Two invited presentations were given by Dr A Peet - 1) response assessment in new drug trials 2) Assessing changes in tumour metabolism using magnetic resonance spectroscopy. A Peet also chaired the session on paediatric functional imaging. This is the main clinical cancer conference in Europe.

2009 Berlin, Germany - 2 invited key note oral presentations Apart from dissemination of information, the conference in 2009 was used to strengthen collaborations and led to the submission of an application for a Network of Excellence to EU FP7 (£12 Million).
2011 Stockholm, Europe - 1 invited key note oral presentation and 2 oral presentations of abstracts
Year(s) Of Engagement Activity 2009,2011
 
Description Invited plenary presentation at ECCO/ESMO Stockholm 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Around 200 delegates attended the presentation

Invitation to write a review article in Nature Reviews Clinical Oncology from the editor who was in the audience.
Year(s) Of Engagement Activity 2011
 
Description Invited presentation to Philips User Group 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Invited presentation to the Philips MRI User Group. We have a research agreement with Philips and the aim was to inform the group of our research successes, ongoing research and discuss new research options.
Year(s) Of Engagement Activity 2018
 
Description Keynote address at Otto Wolf Symposium 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Invited presentation at UCL, GOSH for a neurosciences symposium.
Year(s) Of Engagement Activity 2017
 
Description Lab tour for family and friends of patient 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Patients, carers and/or patient groups
Results and Impact A family had undertaken a fundraising canoe trip and we had bought a tissue microarrary with it. The family and friends attended the university for a presentation and lab tour.
Year(s) Of Engagement Activity 2018
 
Description Press Release - BBC 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Dr Peet took part in a press release for BBC television involving a family with a child having a brain tumour who had raised funds for the group's research.

Made the general public aware of the research which is being undertaken.
Year(s) Of Engagement Activity 2008
 
Description Radio Interview The Naked Scientists 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? Yes
Geographic Reach Regional
Primary Audience Media (as a channel to the public)
Results and Impact The interview was broadcast on BBC Cambridge radio station and a podcast on their website

Broad dissemination to a technically minded public audience.
Year(s) Of Engagement Activity 2011
 
Description Vice Chair of ISMRM Pediatric Study Group 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact This was the inaugural meeting of the Pediatric Study Group as part of the International Society of Magnetic Resonance in Medicine. The meeting took place at the ISMRM Annual Meeting in Singapore. It consisted of plenary presentation, short presentations by junior researchers and poster presentations together with an open debate on future directions.
Year(s) Of Engagement Activity 2016
 
Description Visit from Nanjing Hospital doctors 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Visit by four doctors, heads of departments including the CMO of Nanjing Hospital in China. They met with the CEO and CMO as well as department heads of Birmingham Children's Hospital. They also visited by research group and listened to a presentation.
Year(s) Of Engagement Activity 2018
 
Description Visit to Nanjing Children's Hospital and Southeast University Nanjing including presentation to paediatric radiology consortium of South China 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I visited Nanjing to formally set up the Joint Imaging Lab between UB, SEU and Nanjing Children's Hospital. Then gave a presentation to the consortium of radiologists from South China and had a meeting with the leaders of this around collaboration.
Year(s) Of Engagement Activity 2018