New in vitro models of DMD by induced pluripotency in patient biopsies and gene knockdown in hESCs

Lead Research Organisation: University of Nottingham
Department Name: School of Human Development

Abstract

Duchenne muscular dystrophy (DMD) is a genetic disorder that ultimately leads to the death of 1 in every 3,000 newborn males. Boys become wheelchair bound in their early teens and succumb to the condition in their twenties to thirties. Heart failure is a major cause of fatality and there is no cure. Years of research have identified the gene that causes DMD and the effects on patients? health have been widely documented. However, the basic understanding of how the condition affects the cells as humans grow and develop is not well understood, particularly in organs such as the heart. In part, our lack of understanding is because we cannot examine the detailed processes that go on either in the cells of young DMD patients or in the cells of their hearts.
This grant application is aimed at combining the knowledge of researchers at the University of Nottingham, who have expertise in human embryonic stem cell (hESC) and induced pluripotent stem cell (iPSC) biology, and those at the University of Newcastle, who have clinical and research experience in studying DMD and how it affects function of muscle cells. Combining these sources of expertise will provide new ways to study DMD and in the future it is hoped that this will lead to new treatments. How will this be achieved?
Whereas hESCs are derived from embryos given by consenting couples undergoing fertility treatment, iPSCs are produced by treating skin cells special proteins. This means that hESCs grown in the laboratory can be made to carry the mutations found in patients with DMD. Alternatively, skin cells from DMD patients can be converted into iPSCs that carry the patient-specific mutation. Remarkably, both hESCs and iPSCs can be grown in a plastic dish in the lab and made to mimic a very early stage of human development, including formation of beating heart cells (cardiomyocytes). This provides a way for the effect of the mutations that cause DMD on early development and cardiomyocyte function to be studied in a carefully controlled laboratory setting. The benefit of this is two-fold. First, it will provide a clearer understanding of the disease process. Second, it will provide a lab-based system that can be used in the future to test new drugs for alleviation and treatment of the symptoms of DMD that affect the heart.

Technical Summary

The prevalence of Duchenne muscular dystrophy (DMD) is ~1 in 3,000 in newborn males and all die as young adults, usually in their twenties to thirties. Although heart failure accounts for at least 25% of fatalities, little is known about how the disorder affects development or function of human cardiomyocytes. Limited knowledge arises from lack of DMD cardiac biopsies, mouse models that show only mild heart anomalies and a complex causative DMD gene, which encodes many dystrophin isoforms. In this proposal we will exploit our wealth of knowledge of culture, genetic modification and cardiomyocyte differentiation of hESCs to produce transgenic lines in which variable numbers of the dystrophin isoforms are knocked down by RNAi. In parallel, we will extend our initial work in which pluripotency has been induced in fibroblasts by lentiviral transduction of transcription factors to now reprogramme fibroblasts from DMD patients. These cellular resources will be used to evaluate the effect of perturbed dystrophin expression on structure and location of the dystrophin-glycoprotein complex (DGC) during differentiation, as well as the impact on cardiomyocyte development, function, survival and electrophysiology. Development of this platform will have far-reaching applications for in vitro modelling of numerous genetic disorders of the heart, providing new routes to innovate and test intervention strategies.

Publications

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publication icon
Braam SR (2010) Genetic manipulation of human embryonic stem cells in serum and feeder-free media. in Methods in molecular biology (Clifton, N.J.)

publication icon
Celiz AD (2014) Materials for stem cell factories of the future. in Nature materials

 
Description EU Stem Cell Patents
Geographic Reach National 
Policy Influence Type Participation in advisory committee
 
Description GTAC
Geographic Reach National 
Policy Influence Type Membership of a guidance committee
 
Description Masters course
Geographic Reach Multiple continents/international 
Policy Influence Type Influenced training of practitioners or researchers
Impact masters levels training in stem cell technologies
 
Description NC3Rs policy document on regenerative medicine (Kevin Shakesheff 2015)
Geographic Reach National 
Policy Influence Type Participation in a national consultation
 
Description Animal Free Research UK "Villains and victims - Deciphering the impact of cell- and mutation-type on cardiac fibrosis".
Amount £146,740 (GBP)
Funding ID AFR19-20293 
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Sector Charity/Non Profit
Country United Kingdom
Start 06/2020 
End 05/2023
 
Description Asha E-term fellowship
Amount £250,000 (GBP)
Organisation Engineering and Physical Sciences Research Council (EPSRC) 
Sector Public
Country United Kingdom
Start 12/2013 
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Description BBSRC IPA
Amount £1,300,000 (GBP)
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 10/2009 
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Description BHF Centre for Regen Med
Amount £2,500,000 (GBP)
Funding ID P47352/ 
Organisation British Heart Foundation (BHF) 
Sector Charity/Non Profit
Country United Kingdom
Start 10/2013 
End 09/2017
 
Description BHF LQTS grant (Elena)
Amount £189,000 (GBP)
Organisation British Heart Foundation (BHF) 
Sector Charity/Non Profit
Country United Kingdom
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Description BHF MyoD grant
Amount £300,000 (GBP)
Funding ID PG/14/59/31000 
Organisation British Heart Foundation (BHF) 
Sector Charity/Non Profit
Country United Kingdom
Start 05/2015 
End 05/2018
 
Description BHF Programme Grant (2 pilot with Sian Harding)
Amount £475,000 (GBP)
Funding ID RG/11/19/29264 
Organisation British Heart Foundation (BHF) 
Sector Charity/Non Profit
Country United Kingdom
Start 04/2012 
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Description BHF programme 2014-17
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Organisation British Heart Foundation (BHF) 
Sector Charity/Non Profit
Country United Kingdom
Start 04/2014 
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Description EMDA
Amount £36,000 (GBP)
Organisation East Midlands Development Agency 
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Start 04/2011 
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Description EP/H028277/1 EPSRC Centre for Innovative Manufacturing in Regenerative Medicine
Amount £5,259,190 (GBP)
Funding ID EP/H028277/1 
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Sector Public
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Start 10/2010 
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Description EPSRC 'Future-Proof' Synthetic Surfaces
Amount £2,300,000 (GBP)
Funding ID EP/H045384/1 
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Sector Public
Country United Kingdom
Start 10/2010 
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Description EPSRC equip - seahorse
Amount £115,000 (GBP)
Organisation Engineering and Physical Sciences Research Council (EPSRC) 
Sector Public
Country United Kingdom
Start 09/2014 
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Description EU - dave
Amount £300,000 (GBP)
Organisation European Commission 
Sector Public
Country European Union (EU)
Start 09/2012 
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Description Heart Res UK (Divya)
Amount £147,000 (GBP)
Funding ID TRP01/12 
Organisation Heart Research UK 
Sector Charity/Non Profit
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Start 01/2013 
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Description Int studentship - spandan
Amount £90,000 (GBP)
Organisation University of Nottingham 
Sector Academic/University
Country United Kingdom
Start 01/2011 
End 12/2014
 
Description MRC capital equipment
Amount £714,000 (GBP)
Funding ID MR/L012618/1 
Organisation Medical Research Council (MRC) 
Sector Public
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Start 10/2013 
End 03/2014
 
Description NC3Rs main panel (Viola)
Amount £515,000 (GBP)
Funding ID NC/K000225/1 
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Sector Charity/Non Profit
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Start 02/2013 
End 02/2016
 
Description NC3Rs-CRACK-IT phase 1
Amount £100,000 (GBP)
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Sector Charity/Non Profit
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Description NC3Rs-CRACK-IT phase 2
Amount £1,000,000 (GBP)
Organisation National Centre for the Replacement, Refinement and Reduction of Animals in Research (NC3Rs) 
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Start 01/2015 
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Description SC4SM cardiomyocyte call
Amount £80,000 (GBP)
Organisation Stem Cells for Safer Medicines (SC4SM) 
Sector Charity/Non Profit
Country United Kingdom
Start 10/2009 
End 09/2010
 
Description University of Nottingham Strategic Development Fund "Nottingham Regenerative Medicine Centre" 1.1.15-31.12.18. £602,140 (PI)
Amount £602,000 (GBP)
Funding ID n/a 
Organisation University of Nottingham 
Sector Academic/University
Country United Kingdom
Start 11/2014 
End 10/2020
 
Description Validating CRISPR-engineered human pluripotent stem cell-derived cardiomyocytes as tools to assist with toxicity profiling, adverse drug reaction notices and black box warnings
Amount £49,996 (GBP)
Funding ID NC/T2T0119 
Organisation National Centre for the Replacement, Refinement and Reduction of Animals in Research (NC3Rs) 
Sector Charity/Non Profit
Country United Kingdom
Start 08/2019 
End 07/2020
 
Description epsrc DTG (Asha)
Amount £150,000 (GBP)
Organisation Engineering and Physical Sciences Research Council (EPSRC) 
Sector Public
Country United Kingdom
Start 10/2011 
End 09/2014
 
Description • BHF Programme Grant RG/15/6/31436: Predicting anti-arrhythmic drug efficacy from the divergent molecular basis of RyR2 dysfunction in genetic arrhythmia syndromes
Amount £1,100,000 (GBP)
Funding ID RG/15/6/31436 
Organisation British Heart Foundation (BHF) 
Sector Charity/Non Profit
Country United Kingdom
Start 10/2015 
End 09/2020
 
Description • BHF Special Project no. SP/15/9/31605. "Coupling gene targeted reporters with fully automated compound library screening to mature hPSC-cardiomyocytes".
Amount £1,100,000 (GBP)
Funding ID SP/15/9/31605 
Organisation British Heart Foundation (BHF) 
Sector Charity/Non Profit
Country United Kingdom
Start 01/2016 
End 12/2019
 
Description • BIRAX grant 04BX14CDLG. Gene targeted optogenetics in hPSC-cardiovascular cells for transplantation into animal models of heart dysfunction. 1.9.15-31.8.18. £400K
Amount £400,000 (GBP)
Funding ID 04BX14CDLG 
Organisation British Council 
Sector Charity/Non Profit
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Start 11/2015 
End 10/2018
 
Description • EPSRC Impact Accelerator Award. A Strategic Partnership with the Australian Cooperative Research Centre for Cell Therapy Manufacturing (CRC-CTM) to Commercialise Stem Cell Culture Polymers
Amount £67,000 (GBP)
Funding ID n/a 
Organisation Engineering and Physical Sciences Research Council (EPSRC) 
Sector Public
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Start 11/2015 
End 02/2017
 
Description • EPSRC Programme Grant EP/N006615/1: 'Next Generation Biomaterials Discovery'. PI: Morgan Alexander. Multiple Co-Is. 1.10.15-30.9.20. £6.6m
Amount £6,600,000 (GBP)
Funding ID EP/N006615/1 
Organisation Engineering and Physical Sciences Research Council (EPSRC) 
Sector Public
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Start 10/2015 
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Description • MRC grant MR/M017354/1: MICA: Development of Metrics and Quality Standards for Scale up of Human Pluripotent Stem Cells. 1.5.15-30.4.17 £1.
Amount £1,200,000 (GBP)
Funding ID MR/M017354/1 
Organisation Medical Research Council (MRC) 
Sector Public
Country United Kingdom
Start 05/2015 
End 04/2017
 
Title F1000Research article (Bhagwan) 
Description Published methods or targeting to safe harbour locus in human pluripotent stem cells 
Type Of Material Technology assay or reagent 
Year Produced 2019 
Provided To Others? Yes  
Impact Interest, views and uptake by other researchers 
URL https://doi.org/10.6084/m9.figshare.c.4573316.v1
 
Title Methods & Protocols article (Mosqeira) 
Description High throughput approaches for non-subjective quantification of cardiomyocyte function in vitro 
Type Of Material Technology assay or reagent 
Year Produced 2019 
Provided To Others? Yes  
Impact Uptake, interest and use by others - one published article followed pretty much exactly the criteria we set out word for word, which gave us extra confidence and validation..! Link to PMID: 31717790 for our article 
 
Title genome editing 
Description genome editing techniques and cell lines 
Type Of Material Cell line 
Year Produced 2013 
Provided To Others? Yes  
Impact developing UK and EU portfolio of research tools and assisting collaborations / grants 
 
Title hiPSC lines 
Description hiPSC lines carrying mutations in long QT syndrome genes 
Type Of Material Cell line 
Year Produced 2011 
Provided To Others? Yes  
Impact 1. Braam SR, Tertoolen L, Casini S, Matsa E, Lu HR, Teisman A, Passier R, Denning C, Gallacher DJ, Towart R, Mummery CL. (2012) Repolarization reserve determines drug responses in human pluripotent stem cell derived cardiomyocytes. Stem Cell Research. 10(1):48-56. PMID: 23089628 
URL http://europepmc.org/abstract/MED/23089628
 
Description Automation (Development of robot) 
Organisation Tecan UK Ltd
Country United Kingdom 
Sector Private 
PI Contribution Bespoke robotic platform for automated culture and differentiation of human stem cells
Collaborator Contribution Role in automation
Impact Publications, grants
Start Year 2010
 
Description BIRAX interactions (Mummery, Gepstein) 
Organisation Technion - Israel Institute of Technology
Country Israel 
Sector Academic/University 
PI Contribution New partnership between three labs
Collaborator Contribution Skills in progenitor cell biology and cell transplantation in vivo
Impact Early stage at present
Start Year 2015
 
Description BRIC - Loughborough 
Organisation Loughborough University
Department Department of Engineering
Country United Kingdom 
Sector Academic/University 
PI Contribution Research collaboration to secure funds
Impact publications
Start Year 2009
 
Description CRACK-IT consortium 
Organisation GlaxoSmithKline (GSK)
Department Safety Assessment GSK
Country United Kingdom 
Sector Private 
PI Contribution consortium working on safety assessment
Collaborator Contribution all active contributors to researcher and academic input
Impact publications and grants
Start Year 2014
 
Description CRACK-IT consortium 
Organisation Leiden University
Country Netherlands 
Sector Academic/University 
PI Contribution consortium working on safety assessment
Collaborator Contribution all active contributors to researcher and academic input
Impact publications and grants
Start Year 2014
 
Description CRACK-IT consortium 
Organisation Pluriomics BV
Country Netherlands 
Sector Private 
PI Contribution consortium working on safety assessment
Collaborator Contribution all active contributors to researcher and academic input
Impact publications and grants
Start Year 2014
 
Description CRACK-IT consortium 
Organisation University of Glasgow
Country United Kingdom 
Sector Academic/University 
PI Contribution consortium working on safety assessment
Collaborator Contribution all active contributors to researcher and academic input
Impact publications and grants
Start Year 2014
 
Description CRACK-IT consortium 
Organisation University of Hamburg
Country Germany 
Sector Academic/University 
PI Contribution consortium working on safety assessment
Collaborator Contribution all active contributors to researcher and academic input
Impact publications and grants
Start Year 2014
 
Description Collaboration with Helen Blau, Stanford, on DMD models 
Organisation Stanford University
Department Stanford Synchrotron Radiation Lightsource
Country United States 
Sector Academic/University 
PI Contribution Expertise, know-how and cell lines (human induced pluripotent stem cells and gene edited counterparts)
Collaborator Contribution Wider datasets leading to complete publication. Rejected from Nature but now under review with Nature Genetics
Impact Publication under review with Nature Genetics
Start Year 2018
 
Description DM1 grant & activity (Lochmuller) 
Organisation Newcastle University
Country United Kingdom 
Sector Academic/University 
PI Contribution Joint BHF grant
Collaborator Contribution Academic collaboration; joint posts; sharing of reagents and data
Impact Joint grant, hence economic
Start Year 2015
 
Description DMD (Lochmuller) 
Organisation Centre for Life
Country United Kingdom 
Sector Charity/Non Profit 
PI Contribution led research
Collaborator Contribution publications & grant
Impact grants & publications
Start Year 2009
 
Description Drug Discovery Catapult 
Organisation Medicines Discovery Catapult
Country United Kingdom 
Sector Private 
PI Contribution Contribution of human pluripotent stem cell and cardiomyocyte disease modelling and drug screening
Collaborator Contribution Knowledge of drug development, complex models and technology platforms
Impact Project started March 2020
Start Year 2019
 
Description Electrophysiology mechanisms (Harmer / Tinker) 
Organisation Queen Mary University of London
Country United Kingdom 
Sector Academic/University 
PI Contribution mechanism of electrophysiology in hPSC-cardiomyocytes
Collaborator Contribution Skills in detailed electrophyioslogy
Impact grants awarded. Papers in progress
Start Year 2014
 
Description Genome editing (Skarnes) 
Organisation The Wellcome Trust Sanger Institute
Country United Kingdom 
Sector Charity/Non Profit 
PI Contribution Skills exchange and training for staff in Nottingham
Collaborator Contribution Carrying out the research and intellectual input
Impact Attracted several new grants of value £2m, introduced new skills to the team, leading to new national / international collaborations
Start Year 2012
 
Description Imperial (Sian Harding) 
Organisation Imperial College London
Country United Kingdom 
Sector Academic/University 
PI Contribution multiple activities on grants, papers, sharing skills, reagents etc
Collaborator Contribution multiple activities on grants, papers, sharing skills, reagents etc
Impact multiple activities on grants, papers, sharing skills, reagents etc
Start Year 2010
 
Description Industrial collaboration (GSK via CRACK-IT) 
Organisation GlaxoSmithKline (GSK)
Department Safety Assessment GSK
Country United Kingdom 
Sector Private 
PI Contribution Facilitated award of NC3Rs CRACK-IT grant
Collaborator Contribution Implicit in the sponsorship and construction of the challenge
Impact Financial, international collaboration, skills sharing, joint PhD students, researcher exchange
Start Year 2013
 
Description LQT (Staniforth) 
Organisation University of Nottingham
Department School of Clinical Sciences Nottingham
Country United Kingdom 
Sector Academic/University 
PI Contribution led collab
Collaborator Contribution publications
Impact publications and grants
Start Year 2010
 
Description MIT (Asha Patel, E-term) 
Organisation Massachusetts Institute of Technology
Country United States 
Sector Academic/University 
PI Contribution E-Term Fellowship and sabbatical
Collaborator Contribution E-Term Fellowship and sabbatical
Impact E-Term Fellowship and sabbatical
Start Year 2013
 
Description MRC-TEL consortium (Manchester, UKSCB, Sanger, TEL) 
Organisation National Institute for Biological Standards and Control (NIBSC)
Country United Kingdom 
Sector Public 
PI Contribution joint grant with industrial partner
Collaborator Contribution joint grant with industrial partner
Impact joint grant with industrial partner
Start Year 2015
 
Description MRC-TEL consortium (Manchester, UKSCB, Sanger, TEL) 
Organisation The Wellcome Trust Sanger Institute
Country United Kingdom 
Sector Charity/Non Profit 
PI Contribution joint grant with industrial partner
Collaborator Contribution joint grant with industrial partner
Impact joint grant with industrial partner
Start Year 2015
 
Description MRC-TEL consortium (Manchester, UKSCB, Sanger, TEL) 
Organisation Tokyo Electron
Country Japan 
Sector Private 
PI Contribution joint grant with industrial partner
Collaborator Contribution joint grant with industrial partner
Impact joint grant with industrial partner
Start Year 2015
 
Description MRC-TEL consortium (Manchester, UKSCB, Sanger, TEL) 
Organisation University of Manchester
Country United Kingdom 
Sector Academic/University 
PI Contribution joint grant with industrial partner
Collaborator Contribution joint grant with industrial partner
Impact joint grant with industrial partner
Start Year 2015
 
Description Maturation grant (Tinker, Smith, Fischer) 
Organisation Queen Mary University of London
Country United Kingdom 
Sector Academic/University 
PI Contribution joint grant and development of equipment, new molecules, cell resources
Collaborator Contribution joint grant and development of equipment, new molecules, cell resources
Impact joint grant and development of equipment, new molecules, cell resources
Start Year 2015
 
Description Maturation grant (Tinker, Smith, Fischer) 
Organisation University of Glasgow
Country United Kingdom 
Sector Academic/University 
PI Contribution joint grant and development of equipment, new molecules, cell resources
Collaborator Contribution joint grant and development of equipment, new molecules, cell resources
Impact joint grant and development of equipment, new molecules, cell resources
Start Year 2015
 
Description Sharing hiPSC lines (Pluriomics) 
Organisation Pluriomics BV
Country Netherlands 
Sector Private 
PI Contribution Provision of hiPSC lines, intellectual input to project
Collaborator Contribution Used our resources and input to carry out project
Impact Braam SR, Tertoolen L, Casini S, Matsa E, Lu HR, Teisman A, Passier R, Denning C, Gallacher DJ, Towart R, Mummery CL. (2012) Repolarization reserve determines drug responses in human pluripotent stem cell derived cardiomyocytes. Stem Cell Research. 10(1):48-56. PMID: 23089628
Start Year 2011
 
Description Spannish patients (Roberto Barriales-Villa) 
Organisation University of A Coruña
Country Spain 
Sector Academic/University 
PI Contribution provision of patient samples to underpin research
Collaborator Contribution provision of patient samples to underpin research
Impact provision of patient samples to underpin research
Start Year 2015
 
Description electrophysiology (CPVT, George / Williams) 
Organisation Cardiff University
Country United Kingdom 
Sector Academic/University 
PI Contribution electrophysiology of cardiomyocytes
Collaborator Contribution Developed to £1.1m BHF grant
Impact Grant secured. Working towards papers
Start Year 2010
 
Description exon skipping (Leiden, Aartsma-Rus) 
Organisation Leiden University
Country Netherlands 
Sector Academic/University 
PI Contribution brought skills to the team not available in Nottingham
Collaborator Contribution reagents and expertise in exon skipping
Impact Publication of paper
Start Year 2011
 
Description surface work (Morgan Alexander, Nottingham) 
Organisation University of Nottingham
Department School of Pharmacy
Country United Kingdom 
Sector Academic/University 
PI Contribution lead the stem cell work of this collaboration
Collaborator Contribution Multiple grant awards and papers
Impact grants and papers
Start Year 2010
 
Description syngenta 
Organisation Syngenta International AG
Country Switzerland 
Sector Private 
PI Contribution stem cell automation
Collaborator Contribution intellectual and financial input
Impact advances in stem cell automation
Start Year 2009
 
Description virus (Dave Darling) 
Organisation King's College London
Country United Kingdom 
Sector Academic/University 
PI Contribution led collab
Collaborator Contribution publications
Impact publications
Start Year 2010
 
Title GET system with James & Kevin 
Description This invention relates to transduction of cargo molecules into living cells, such as protein transduction, in particular a delivery molecule for transduction of a cargo into a cell comprising: a cargo-binding molecule and/or a cargo; a glycosaminoglycan (GAG) binding element, which is capable of binding to GAG on the surface of the cell; and a protein transduction domain. Methods of transduction, methods of producing or modifying cargo for transduction, delivery molecules for transduction and methods of treatment using transduction, or using transduced cells are also provided. 
IP Reference WO2015092417 
Protection Patent application published
Year Protection Granted 2015
Licensed No
Impact Scientific development with view to licensing.
 
Title New synthetic culture surfaces for human embryonic stem cells 
Description Patent filed to cover new synthetic substrate to culture human embryonic stem cells 
IP Reference  
Protection Protection not required
Year Protection Granted 2009
Licensed No
Impact Negotiations with major plasticware companies in progress through Nottingham's tech trasnfer office
 
Title Raman 
Description optical spectra for cardiomyocytes using Raman microspectroscopy defined 
IP Reference GB1018628.6 
Protection Patent application published
Year Protection Granted 2010
Licensed Commercial In Confidence
Impact linking into partners within the Bioprocess Research Industry Club
 
Description BBC East Midlands Today - BHF education on heart disease 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Media (as a channel to the public)
Results and Impact Interview with BBC East Midlands Today to educate on importance for research for heart disease
Year(s) Of Engagement Activity 2016
 
Description BBC News 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Media (as a channel to the public)
Results and Impact more interest in lab / education

interest in lab / education
Year(s) Of Engagement Activity 2015
 
Description BBC radio Nottingham (Heart-light) 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Media (as a channel to the public)
Results and Impact Public engaged on new research grant
Year(s) Of Engagement Activity 2015
 
Description BHF - Welcome booklet imaging (Sorrel Bunting Oct 2016) 
Form Of Engagement Activity A magazine, newsletter or online publication
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact Information booklet commissioned by BHF and Wellcome Trust to support research against cardiovascular disease
Year(s) Of Engagement Activity 2016
 
Description BHF Visit Outreach for Sorrel Bunting (Nov 2016) 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Supporters
Results and Impact Group of 20 workers from BHF, including shop-runners, fund raisers, office workers
Year(s) Of Engagement Activity 2016
 
Description BHF news advert / article 
Form Of Engagement Activity A magazine, newsletter or online publication
Part Of Official Scheme? Yes
Type Of Presentation Paper Presentation
Geographic Reach National
Primary Audience Media (as a channel to the public)
Results and Impact Advertising in national newspaper

to early to tell but part of BHF effort to continually raise funds
Year(s) Of Engagement Activity 2012
 
Description BHF public information booklet on research 
Form Of Engagement Activity A magazine, newsletter or online publication
Part Of Official Scheme? Yes
Type Of Presentation Paper Presentation
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact British people with interest in heart disease engaged

to early to be determined but acts as part of continual BHF effort to raise funds
Year(s) Of Engagement Activity 2012
 
Description BHF visit July 2018 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Half day workshop held in Nottingham to showcase work being done with presentations, tours and Q&A session. Invited people who represent patient groups, public, charities
Year(s) Of Engagement Activity 2018
 
Description Biohacking with Jonathan Ball on Radio 4 
Form Of Engagement Activity A broadcast e.g. TV/radio/film/podcast (other than news/press)
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Media (as a channel to the public)
Results and Impact Radio 4 broadcast on 'biohacking' to promote advances in science using stem cells and gene editing
Year(s) Of Engagement Activity 2018
URL https://www.bbc.co.uk/programmes/b0bgq182
 
Description Blog in Huffington post 
Form Of Engagement Activity A magazine, newsletter or online publication
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Media (as a channel to the public)
Results and Impact Blog in Huffington Post which promoted new grant - global reach
Year(s) Of Engagement Activity 2015
URL http://www.huffingtonpost.co.uk/simon-gillespie/valentines-day_b_6669914.html?utm_hp_ref=science
 
Description Channel 4 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Media (as a channel to the public)
Results and Impact Featured on channel 4 documentary

disseminated to wider public
Year(s) Of Engagement Activity 2010
 
Description Invited conference presentation during 2016-2017 reporting period 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Presented at >20 international conferences in Europe, Middle East, Asia and USA, each having 50 to 500 delegates from professional academic, public, media, patient and industry
Year(s) Of Engagement Activity 2016,2017
 
Description Media (as a channel to the public) 
Form Of Engagement Activity A magazine, newsletter or online publication
Part Of Official Scheme? Yes
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact - Provision of short articles to magazines such as BioWorld International, BBSRC business, Hospital Solutions - Interview with newspapers, such as Daily Mail, Telegraph etc organised through the Science Media Centre in London - Interviewed and filmed by BBC Scotland 6 O'clock news and BBC / ITV local news stations

Increased public perception and acceptance of the importance of stem cells. Increased application rates from PhD students to our unit
Year(s) Of Engagement Activity 2006,2007,2008,2009
 
Description Nottingham press release (EPSRC £6.5m project) 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Media (as a channel to the public)
Results and Impact Press release leading to new interactions
Year(s) Of Engagement Activity 2015
URL http://www.nottingham.ac.uk/news/pressreleases/2015/august/3d-vision-for-the-next-generation-of-biom...
 
Description Nottingham press release (heart light) 
Form Of Engagement Activity A magazine, newsletter or online publication
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Media (as a channel to the public)
Results and Impact Press release on new grant
Year(s) Of Engagement Activity 2015
URL http://www.nottingham.ac.uk/news/pressreleases/2015/february/light-sensitive-proteins-could-help-men...
 
Description Nottingham press release (new biomaterials) 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Media (as a channel to the public)
Results and Impact nottingham press release on new publication. Led to additional funding and interaction
Year(s) Of Engagement Activity 2015
URL http://www.nottingham.ac.uk/news/pressreleases/2015/july/new-material-forges-the-way-for-stem-cell-f...
 
Description Observer & Guardian on Sunday 2014 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact Description of research to National newspapers

follow up enquiries and public engagement
Year(s) Of Engagement Activity 2014
 
Description Public engagement day 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact 250 members of the public attended the British Society for Gene Therapy public day in Brighton

active engagement and enthusiasm from members of the public
Year(s) Of Engagement Activity 2011
 
Description SkyNews 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Media (as a channel to the public)
Results and Impact Watched by 13 million people

interest in research group
Year(s) Of Engagement Activity 2015
 
Description observer 
Form Of Engagement Activity A magazine, newsletter or online publication
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact Interview with Observer on hiPSC-based cardiovascular work

national press and engagement with public
Year(s) Of Engagement Activity 2011
 
Description public engagement day 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? Yes
Type Of Presentation Keynote/Invited Speaker
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact The engagement day was attended by approx 250 members of the public

good attendance and feedback will mean the event will be run again in 2013.
Year(s) Of Engagement Activity 2011