Harnessing Self assembly to generate a spectrum of multi-functionalised nanoparticles for multimodal imaging

Lead Research Organisation: University of Nottingham
Department Name: Sch of Chemistry

Abstract

We will use a protein that can self assemble from its subunits into a capsule as a vehicle for imaging inside patients using magnetic resonance or tomography and in the longer term delivering drugs to specific tissues such as tumours. As the individual building blocks of the protein capsule can be modified in different ways, the capsule can be optimised on an individual basis based on patient need.

Technical Summary

Ferritin subunits containing un-natural azido bearing amino acids will be produced and MRI, fluorescence and PET probes added using click chemistry. The subunits will then be mixed in known ratios and apoferritin protein capsules will self assemble. These nanoparticles will be characterised by DLS, TEM, fluorescence and MRI/PET before being evaluated for their localisation and imaging properties in mice.
 
Description Anne McLaren Fellowship (for L. Turyanska)
Amount £70,000 (GBP)
Organisation University of Nottingham 
Sector Academic/University
Country United Kingdom
Start 01/2008 
End 12/2010
 
Title human and mouse apoferritin with un-natural amino acids 
Description human and mouse apoferritin light subunits with azidohomoaanine at specific positions have been produced. These will allow specific attacment of fluorophores or other ligands on to the azido group using click chemistry. 
Type Of Material Technology assay or reagent 
Year Produced 2010 
Provided To Others? Yes  
Impact Too early to say 
 
Description Biological evaluation of Quantum Dots-apofferitin composites 
Organisation University of Nottingham
Country United Kingdom 
Sector Academic/University 
PI Contribution Preparation of the apoferritin/quantum dots
Collaborator Contribution Testing the toxicity of our apoferritin/Quantum dot composits against a panel of cell lines.
Impact TURYANSKA, L., BRADSHAW, T. D., SHARPE, J., LI, M., MANN, S., THOMAS, N. R. and PATANE, A., 2009. The Biocompatibility of Apoferritin-Encapsulated PbS Quantum Dots. Small, 5(15), 1738-1741.
Start Year 2008
 
Description Characterisation of apoferritin QDs by TEM 
Organisation University of Bristol
Department School of Chemistry
Country United Kingdom 
Sector Academic/University 
PI Contribution Preparation of apoferritin-QDs
Collaborator Contribution TEM studies on our apoferritin/quantum dot strutures
Impact 19360723 and HENNEQUIN, B., TURYANSKA, L., BEN, T., BELTRAN, A. M., MOLINA, S. I., LI, M., MANN, S., PATANE, A. and THOMAS, N. R., 2008. Aqueous Near-Infrared Fluorescent Composites Based on Apoferritin-Encapsulated PbS Quantum Dots. Advanced Materials, 20(19), 3592-3596
Start Year 2008
 
Description Univ. Bristol TEM studies 
Organisation University of Bristol
Department School of Chemistry
Country United Kingdom 
Sector Academic/University 
PI Contribution Provided the Apoferritin (human and mouse) PbS quantum dots for TEM studies at Bristol
Collaborator Contribution Characterisation of hybrd materials we have made by TEM.
Impact Publication on toxicity of composites under preparation
Start Year 2009
 
Description UoN - Ultracentrifugation studies 
Organisation University of Nottingham
Department School of Biosciences
Country United Kingdom 
Sector Academic/University 
PI Contribution Apoferritin-QD and engineered apoferritins studied for their self assembly properties by analytical ultracentrifugation (AUC) studies
Collaborator Contribution Characterisation of materials we have produced by utracentrifugation
Impact Paper in prearation
Start Year 2010