Chemical genetics with phosphoinositide analogues and PH-domains to identify downstream signalling elements

Lead Research Organisation: Imperial College London
Department Name: Dept of Chemistry

Abstract

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Publications

10 25 50
 
Title 2-O-acetic acid IP4 
Description 2-O-acetic acid inositol 1,3,4,5-tetrakisphosphate, and related analogues, were prepared to fit the E17A mutant of PKB PH-domain 
Type Of Material Technology assay or reagent 
Year Produced 2008 
Provided To Others? Yes  
Impact These materials were supplied to Rudiger Woscholski's lab to determine if they were phosphatase substrates or inhibitors. These results are yet to be published 
 
Title Bumpy IP3 and IP4 analogues 
Description Novel analogues of inositol 1,4,5-trisphosphate and 1,3,4,5-tetrakisphosphate with various alkyl groups replacing the 4-H were prepared 
Type Of Material Model of mechanisms or symptoms - human 
Year Produced 2007 
Provided To Others? Yes  
Impact The binding of 4-C-methyl-IP3 and -IP4 to IP3 receptor have been reported (PMID 19343261), but further studies in Geert Bultynk's lab have yet to be reported. We have also supplied these materials to Rudiger Woscholski's lab to determine whether these materials were phosphatase substrates. These results are also yet to be published. 
URL http://europepmc.org/abstract/MED/19343261
 
Description Bumpy IPn binding to IP3R receptor 
Organisation Catholic University of Louvain
Department Laboratory of Molecular and Cellular Signaling Louvain
Country Belgium 
Sector Academic/University 
PI Contribution The bumpy IP3 and IP4 analogues, prepared as part of the MRC fellowship, were sent to the laboratory of Geert Bultynk
Collaborator Contribution Testing many IP3 and IP4 analogues as IP3 receptor antagonists
Impact PMID: 19343261 shows the first results, but significantly more work has been done which I plan to publish in due course.
Start Year 2008