MRC/WT Human Tissue Resource

Lead Research Organisation: Newcastle University
Department Name: Institute of Human Genetics

Abstract

Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.

Publications

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Alfakir M (2012) The temporal and spatial expression patterns of ABCG2 in the developing human heart. in International journal of cardiology

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Bakircioglu M (2011) The essential role of centrosomal NDE1 in human cerebral cortex neurogenesis. in American journal of human genetics

 
Title HUDSEN Human Spatial Gene Expression Database 
Description database developed in collaboration (as part of a different grant) with the eMAP team at the MRC Human Geneitcs Unit Edinburgh and based on the eMAGE database. The HUDSEN dataase contains human gene expression patterns mapped to 3D models of early human developmental stages. 
Type Of Material Biological samples 
Year Produced 2009 
Provided To Others? Yes  
Impact The database is freely available to the scientific community. Data from it has been used publications. 
URL http://database.hudsen.org/
 
Title Human Developmental Studies Network (HuDSeN) atlas and human gene expression spatial database 
Description The HuDSeN Human Gene Expression Spatial Database (HuDSeN GXD) is based on a series of digital 3D models through 12 stages of human development (Carnegie Stages [CS] 12-23; approx 26-56 days post conception [dpc]). Virtual 3D anatomical models are generated from intact specimens using optical projection tomography (OPT, Sharpe et al., (2002) Science 296: 541-5). Using MAPaint software, developed Prof Richard Baldock and his colleagues in the Edinburgh Mouse Atlas team (http://www.emouseatlas.org/emage/), selected gene expression data, gathered using standard methods of in situ hybridisation and immunohistochemistry, are mapped to a representative 3D model for each chosen Carnegie stage. In these models, anatomical domains, defined on the basis of morphological landmarks and comparative knowledge of expression patterns in vertebrates, are linked to a developmental neuroanatomic ontology. Currently, the 3D models and a preliminary set of anatomical domains and ontology are available on the atlas pages along with gene expression data from approximately 85 genes in the HUDSEN Human Spatial Gene Expression Database (www.hudsen.org). 
Type Of Material Database/Collection of data 
Year Produced 2008 
Provided To Others? Yes  
Impact The HuDSeN GXD enables researchers using HDBR material to make much of their data publicly available. 
URL http://www.hudsen.org
 
Description Abnormal Cortical Development 
Organisation Harvard University
Department Harvard Medical School
Country United States 
Sector Academic/University 
PI Contribution provision of human fetal and embryonic tissue for in-house gene expression service
Collaborator Contribution transfer of expertise
Impact PMID: 17191205 PMID: 20118933
 
Description Abnormal cortical development-NHEJ1 
Organisation Aix-Marseille University
Country France 
Sector Academic/University 
PI Contribution provision of human embryonic material for in-house gene expression services
Impact PMID: 17191205
 
Description Cortical spinal system development, plasticity and repair 
Organisation Newcastle University
Department Department of Child Health
Country United Kingdom 
Sector Academic/University 
PI Contribution provision of human embryonic and fetal tissue, intellectual contribution to direction of project
Collaborator Contribution transfer of expertise
Impact PMID: 17965125 PMID: 18973570 PMID: 21060114 PMID: 20579172
 
Description DLG3 in causation of mental retardation 
Organisation University of Cambridge
Department Cambridge Institute for Medical Research (CIMR)
Country United Kingdom 
Sector Academic/University 
PI Contribution provision of human embryo and fetal tissue for in-house gene expression services
Impact PMID: 17013395
 
Description FRMD7-brain 
Organisation Medical Research Council (MRC)
Department MRC Toxicology Unit
Country United Kingdom 
Sector Public 
PI Contribution provision of human embryonic and fetal tissues, gene expression studies, data analysis and interpretation
Impact PMID: 19892780
Start Year 2008
 
Description KIF21A and CHN1 expression 
Organisation Harvard University
Department Harvard Medical School
Country United States 
Sector Academic/University 
PI Contribution provision of human embryonic and fetal tissues for in-house gene expression services
Collaborator Contribution exchange of expertise
Impact PMID: 18653847 PMID: 20074521
Start Year 2006
 
Description PLA2G6 studies 
Organisation Oregon Health and Science University
Department Department of Molecular and Medical Genetics
Country United States 
Sector Academic/University 
PI Contribution provision of human embryonic and fetal tissues, undertaking gene expression studies, interpretation and analysis of data
Impact PMID: 20813170
Start Year 2006
 
Description Roberts syndrome 
Organisation Newcastle University
Department Institute of Genetic Medicine
Country United Kingdom 
Sector Academic/University 
PI Contribution provision of human embryonic and fetal tissue and carrying out of gene expression studies
Impact publication
 
Description heart development 
Organisation Newcastle University
Department Institute of Genetic Medicine
Country United Kingdom 
Sector Academic/University 
PI Contribution provision of tissues
Collaborator Contribution exchange of expertise
Impact PMID: 21111494
Start Year 2006
 
Description microcephaly 
Organisation University of Cambridge
Department Department of Medical Genetics
Country United Kingdom 
Sector Academic/University 
PI Contribution provision of human embryonic and fetal tissues
Collaborator Contribution transfer of expertise
Impact PMID: 20890279
 
Description subplate 
Organisation University of Oxford
Department Department of Physiology, Anatomy and Genetics
Country United Kingdom 
Sector Academic/University 
PI Contribution provision of human embryonic and fetal tissues
Collaborator Contribution transfer of expertise
Impact PMID: 20727056
Start Year 2008
 
Description Meet the scientist event at the Body Worlds Exhibition, International Centre for Life 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact There were three parts to the activity- DVD showing stages of human embryonic development, microscope and slides with stained sections and computer-based 3D model with painted anatomical domains. The activity was situated near the "prenatal development" section of the Bodyworlds exhibition and provoked much discussion with the many children and adults who stopped to look and ask questions.

As activity took place in a science museum and was aimed at the general public, it is difficult to directly attribute impacts.
Year(s) Of Engagement Activity 2014
 
Description contribution to A-level student open day at Institute of Genetic Medicine, Newcastle University 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Group members prepared a hands-om session using a microscope and sets of slides. In the session they asked students to identify human from mouse sections and to explain why they thought these differences were important. Session led to many questions from students who were interested and involved in the activity.

Increased requests for work experience in my lab and request to contribute to a similar open day next year.
Year(s) Of Engagement Activity 2014