Support for a FACS Cell Sorter at the University of Leicester

Lead Research Organisation: University of Leicester

Abstract

Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.

Technical Summary

The University of Leicester was awarded £536K for a FACS cell sorter. There is a major and urgent need across researchers in Medical and Biological Sciences and the MRC Toxicology Unit at Leicester for this equipment, which can be used to physically isolate specific cell types from heterogenous cell populations in translational projects. The availability of a new system is crucial to the success of several areas of translational research, most notably the development of stem cell technology. In addition, as a core facility it will significantly enhance the output of a number of ongoing research projects.

Publications

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Hill CU (2015) Inhibition of prostate cancer cell growth by 3',4',5'-trimethoxyflavonol (TMFol). in Cancer chemotherapy and pharmacology

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Irving GR (2011) Curcumin: the potential for efficacy in gastrointestinal diseases. in Best practice & research. Clinical gastroenterology

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Núñez-Sánchez MÁ (2016) In vivo relevant mixed urolithins and ellagic acid inhibit phenotypic and molecular colon cancer stem cell features: A new potentiality for ellagitannin metabolites against cancer. in Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

 
Description CRUK Programme grant
Amount £1,400,000 (GBP)
Organisation Cancer Research UK 
Sector Charity/Non Profit
Country United Kingdom
Start 04/2011 
End 04/2016
 
Description Clinical Fellow supplement
Amount £67,591 (GBP)
Organisation Hope Against Cancer (Rutland and Leicestershire) 
Sector Charity/Non Profit
Country United Kingdom
Start 10/2017 
End 09/2018
 
Description Clinical PhD fellowship
Amount £133,500 (GBP)
Organisation Hope Against Cancer (Rutland and Leicestershire) 
Sector Charity/Non Profit
Country United Kingdom
Start 10/2015 
End 09/2017
 
Description Experimental Cancer Medicine Centre
Amount £1,450,000 (GBP)
Funding ID C10604/A25151 
Organisation Cancer Research UK 
Sector Charity/Non Profit
Country United Kingdom
Start 04/2017 
End 03/2022
 
Description Experimental Cancer Medicine Centre (ECMC) Network funding
Amount £1,450,000 (GBP)
Organisation Experimental Cancer Medicine Centre Network (ECMC) 
Sector Charity/Non Profit
Country United Kingdom
Start 04/2017 
End 03/2022
 
Description Experimental Cancer Medicine Centre Programme
Amount £1,400,000 (GBP)
Organisation Cancer Research UK 
Sector Charity/Non Profit
Country United Kingdom
Start 07/2012 
End 07/2017
 
Description LLR project grant scheme
Amount £250,000 (GBP)
Organisation Leukaemia and Lymphoma Research 
Sector Charity/Non Profit
Country United Kingdom
Start 07/2011 
End 07/2014
 
Description PhD Studentship
Amount £90,000 (GBP)
Organisation Cancer Prevention Research Trust 
Start 12/2017 
End 11/2020
 
Description PhD Studentship
Amount £99,245 (GBP)
Organisation British Lung Foundation (BLF) 
Sector Charity/Non Profit
Country United Kingdom
Start 10/2016 
End 09/2019
 
Description Project Grant
Amount £218,798 (GBP)
Organisation Leukaemia and Lymphoma Research 
Sector Charity/Non Profit
Country United Kingdom
Start 10/2011 
End 07/2015
 
Description Training & Career Development Board - Research Bursary
Amount £13,175 (GBP)
Organisation Cancer Research UK 
Sector Charity/Non Profit
Country United Kingdom
Start 10/2014 
End 11/2015
 
Description Analysing t-cell populations from patient blood after fish oil application on patients with pancreatitis 
Organisation Leicester Royal Infirmary
Department Surgery
Country United Kingdom 
Sector Hospitals 
PI Contribution Collaborating in clincal trial and providing expertise and infrastructure in laboratory with FACS machine
Collaborator Contribution Design of clincal trial and patient enrollment. Contribution of time of research fellow to FACS analysis
Impact Too early
Start Year 2011
 
Description Apoptosis and cell cycle analysis after UVA,UVB induced cytotoxicity by selected drugs of Hut78 human T-cell lymphoma 
Organisation University of Leicester
Department Department of Cancer Studies
Country United Kingdom 
Sector Academic/University 
PI Contribution advice on use of FACS and technical supervsion fo research
Collaborator Contribution design of study
Impact Too early
Start Year 2011
 
Description Apoptosis and cell cycle analysis after UVA,UVB treatment of skin cells 
Organisation University of Leicester
Department Department of Cancer Studies
Country United Kingdom 
Sector Academic/University 
PI Contribution support for use of FACS and guidance on appropriate use
Collaborator Contribution design of project
Impact Too early
Start Year 2011
 
Description Apoptosis and cell cycle analysis of human melanoma and lymphoma 
Organisation University of Leicester
Department Department of Cancer Studies
Country United Kingdom 
Sector Academic/University 
PI Contribution advice on use of FACS in programme and supervision of running of FACS sorting
Collaborator Contribution Design of project
Impact Too early
Start Year 2010
 
Description Assessing expression levels of C1GaIT1, and its chaperone protein, IgA and non-IgA in B cells 
Organisation University of Leicester
Department Department of Geology
Country United Kingdom 
Sector Academic/University 
PI Contribution Support with experimental design and running the FACS sorter.
Collaborator Contribution Study design, generation of samples and interpretation of data
Impact None yet
Start Year 2016
 
Description Blood analysis of fish oil treated patients with pancreatic cancer 
Organisation Leicester General Hospital
Department Surgery and Pathology
Country United Kingdom 
Sector Hospitals 
PI Contribution joint writing of prject protocol and supervision of FACS sorting. Patient recruitemnt
Collaborator Contribution Joint protocol writing and patient recruitmetn
Impact Too early
Start Year 2011
 
Description Characterization of mesenchymal stem cells isolated from umbilical cord 
Organisation University of Leicester
Department Department of Cardiovascular Sciences
Country United Kingdom 
Sector Academic/University 
PI Contribution contribution to research plan and running of FACS sorter
Collaborator Contribution lead in design of research
Impact Too early
Start Year 2011
 
Description Determine mechanism of decrease in smooth muscle actin in fibrocytes when cocultured with ASM 
Organisation University of Leicester
Department Department of Infection, Immunity and Inflammation
Country United Kingdom 
Sector Academic/University 
PI Contribution input to design of research project and running of FACS sorter
Collaborator Contribution design of projest and colelction fo samples
Impact Too early
Start Year 2010
 
Description Developing Ang2 ligand trap by using Tie2 ectodomain mutants expressed in transfected DT40 cells. Sorting of mutants 
Organisation University of Leicester
Department Department of Cardiovascular Sciences
Country United Kingdom 
Sector Academic/University 
PI Contribution Practical support for us of FACS machine
Collaborator Contribution Design of study and sample provision
Impact Too early
Start Year 2010
 
Description Directed evolution and structure/function analysis of proteins displayed on cell surface of B cells, DT40 and HEK293 cells 
Organisation University of Leicester
Department Department of Cardiovascular Sciences
Country United Kingdom 
Sector Academic/University 
PI Contribution Supervision of experiments using FACS sorter
Collaborator Contribution Stduy design and provision of specimens
Impact Manuscript in preparation
Start Year 2010
 
Description Expression And Functional Significance Of The Endocannabinoid System In Human Spermatozoa 
Organisation University of Leicester
Department Leicester Medical School
Country United Kingdom 
Sector Academic/University 
PI Contribution support to research group in use of FACS sorter and advice on optimal separation of stem cells
Collaborator Contribution Has establsihed use of stem cell biology in endocannabinoid research and potential use in infertility
Impact Has already led to presentation and abstract publication: "Expression And Functional Significance Of The Endocannabinoid System In Human Spermatozoa" FERTILITY AND STERILITY: Volume: 96 Issue: 3 Supplement: 1 Pages: S87-S87 Published: SEP 2011 This is multidisciplinary between Reproductive Sciences and Oncology and is to be extended to other istitutions in UK and abroad.
Start Year 2010
 
Description Identification of specific cells types from lung that express KRAS-G12D by nasal Cre delivery. 
Organisation University of Leicester
Department Department of Genetics
Country United Kingdom 
Sector Academic/University 
PI Contribution Help with the isolation by FACS sorting of specific cell populations, through assistance with the experimental design and running of the instrument.
Collaborator Contribution Design of the study, generating the samples, performing experiments and interpreting data.
Impact None yet
Start Year 2016
 
Description Identifying which cellular subtype is responsible for producing elevated concentrations of IFNalpha in PBMCs from COPD patients compared to controls 
Organisation University of Leicester
Department Department of Infection, Immunity and Inflammation
Country United Kingdom 
Sector Academic/University 
PI Contribution Study design and running of FACS sorter
Collaborator Contribution Primary designers of research project and collection of samples
Impact Too early
Start Year 2010
 
Description Potential of Repurposed Drugs in Preventing Malignant Pleural Mesothelioma. 
Organisation University of Birmingham
Country United Kingdom 
Sector Academic/University 
PI Contribution Conceived the idea and designed the project. Conducted the work and interpreted data.
Collaborator Contribution Provision of a library of repurposed drugs and associated data.
Impact Oral and poster conference presentations.
Start Year 2015
 
Description Sorting airway epithelial stem cells from foetal human lung tissue stained with Hoechst 3342 
Organisation University of Leicester
Department Department of Infection, Immunity and Inflammation
Country United Kingdom 
Sector Academic/University 
PI Contribution use of FACS sorter and advice on protocol design
Collaborator Contribution lead in designing ersearch prokject and collection specimens
Impact Too early
Start Year 2011
 
Description Sorting cancer stem cells from pancreatic tumors 
Organisation Leicester General Hospital
Department Surgery and Pathology
Country United Kingdom 
Sector Hospitals 
PI Contribution Support to train in use of FACS sorter and advice on optimal sorting techniques
Collaborator Contribution Has extended models of use of stem cell sorting to pancreas
Impact Is being used to rpedict response to therapy of pancreas cancer. Multidisciplinary with Pathology Department
Start Year 2009
 
Description Sorting leucocyte rich plasma for nociceptin receptor for mRNA analysis 
Organisation Leicester Royal Infirmary
Department Anaesthetics
Country United Kingdom 
Sector Hospitals 
PI Contribution Advice and support throughout learning period of using Cell sorter. Contribution of technical support.
Collaborator Contribution Will lead to joint publications and potential for joint grant application
Impact Presentation at meeting and published abstract: Use of preparative fluorescence-activated cell sorting (FACS) to profile opioid receptor and peptide mRNA expression on human granulocytes, lymphocytes, and monocytes. Annual Meeting of the Anaesthetic-Research-Society Location: Univ Nottingham, Nottingham, ENGLAND: JUL 01-02, 2010 BRITISH JOURNAL OF ANAESTHESIA Volume: 105 Issue: 5 Pages: 707-708 Published: NOV 2010 This was multidisciplinary research with Anaesthetic department.
Start Year 2009
 
Description Sorting to establish the stable transfection of clones 
Organisation University of Leicester
Department Department of Infection, Immunity and Inflammation
Country United Kingdom 
Sector Academic/University 
PI Contribution Support with experimental design and running the FACS sorter.
Collaborator Contribution Study design, generation of samples and interpretation of data
Impact None yet
Start Year 2016
 
Description CRUK fundraisers annual meeting Leicester 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Participants in your research and patient groups
Results and Impact 75 attendees at this talk on research in unit and large number of questions and discussion

Visits to laboratories provided subsequently and requests for more talks.
Year(s) Of Engagement Activity 2010
 
Description Local cancer fundraising group - Hope Leicester 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Participants in your research and patient groups
Results and Impact Presentation to 60-80 attendees on use of stem cells in research and cancer prevention research. Many questions and extensive discussion.

Invited back for next year as felt to be of great interest
Year(s) Of Engagement Activity 2010,2011
 
Description School visit 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? Yes
Geographic Reach Regional
Primary Audience Schools
Results and Impact 40 pupils attnded each event as part of University outreach intiative funded as part of GENIE programme. Talk on research in cacner prevention and role of stem cells in research. Many questions and considerable interest.

High interest reported by school and organsiers fo events.
Year(s) Of Engagement Activity 2010,2011
 
Description Science evening at Lutterworth High School 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact 50 schoolchildren and parents attended and sparked considerabel interest among both groups with questions and discussion

Very well received by college staff who made the invitation
Year(s) Of Engagement Activity 2011