Support for a FACS Cell Sorter at the University of Leicester
Lead Research Organisation:
University of Leicester
Department Name: UNLISTED
Abstract
Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.
Technical Summary
The University of Leicester was awarded £536K for a FACS cell sorter. There is a major and urgent need across researchers in Medical and Biological Sciences and the MRC Toxicology Unit at Leicester for this equipment, which can be used to physically isolate specific cell types from heterogenous cell populations in translational projects. The availability of a new system is crucial to the success of several areas of translational research, most notably the development of stem cell technology. In addition, as a core facility it will significantly enhance the output of a number of ongoing research projects.
People |
ORCID iD |
Publications
Irving GR
(2011)
Curcumin: the potential for efficacy in gastrointestinal diseases.
in Best practice & research. Clinical gastroenterology
Hill CU
(2015)
Inhibition of prostate cancer cell growth by 3',4',5'-trimethoxyflavonol (TMFol).
in Cancer chemotherapy and pharmacology
Núñez-Sánchez MÁ
(2016)
In vivo relevant mixed urolithins and ellagic acid inhibit phenotypic and molecular colon cancer stem cell features: A new potentiality for ellagitannin metabolites against cancer.
in Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association
Wright AK
(2015)
Toll-like receptor 9 dependent interferon-a release is impaired in severe asthma but is not associated with exacerbation frequency.
in Immunobiology
Howells LM
(2011)
Curcumin ameliorates oxaliplatin-induced chemoresistance in HCT116 colorectal cancer cells in vitro and in vivo.
in International journal of cancer
Norris L
(2013)
The role of cancer stem cells in the anti-carcinogenicity of curcumin.
in Molecular nutrition & food research
Haqq J
(2016)
Targeting pancreatic cancer using a combination of gemcitabine with the omega-3 polyunsaturated fatty acid emulsion, Lipidem™.
in Molecular nutrition & food research
Empl MT
(2018)
Effects of a Grapevine Shoot Extract Containing Resveratrol and Resveratrol Oligomers on Intestinal Adenoma Development in Mice: In Vitro and In Vivo Studies.
in Molecular nutrition & food research
| Description | CRUK Programme grant |
| Amount | £1,400,000 (GBP) |
| Organisation | Cancer Research UK |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 04/2011 |
| End | 04/2016 |
| Description | Clinical Fellow supplement |
| Amount | £67,591 (GBP) |
| Organisation | Hope Against Cancer (Rutland and Leicestershire) |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 10/2017 |
| End | 09/2018 |
| Description | Clinical PhD fellowship |
| Amount | £133,500 (GBP) |
| Organisation | Hope Against Cancer (Rutland and Leicestershire) |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 10/2015 |
| End | 09/2017 |
| Description | Experimental Cancer Medicine Centre |
| Amount | £1,450,000 (GBP) |
| Funding ID | C10604/A25151 |
| Organisation | Cancer Research UK |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 04/2017 |
| End | 03/2022 |
| Description | Experimental Cancer Medicine Centre (ECMC) Network funding |
| Amount | £1,450,000 (GBP) |
| Organisation | Experimental Cancer Medicine Centre Network (ECMC) |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 04/2017 |
| End | 03/2022 |
| Description | Experimental Cancer Medicine Centre Programme |
| Amount | £1,400,000 (GBP) |
| Organisation | Cancer Research UK |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 07/2012 |
| End | 07/2017 |
| Description | LLR project grant scheme |
| Amount | £250,000 (GBP) |
| Organisation | Leukaemia and Lymphoma Research |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 07/2011 |
| End | 07/2014 |
| Description | PhD Studentship |
| Amount | £99,245 (GBP) |
| Organisation | British Lung Foundation (BLF) |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 10/2016 |
| End | 09/2019 |
| Description | PhD Studentship |
| Amount | £90,000 (GBP) |
| Organisation | Cancer Prevention Research Trust |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 12/2017 |
| End | 11/2020 |
| Description | Project Grant |
| Amount | £218,798 (GBP) |
| Organisation | Leukaemia and Lymphoma Research |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 10/2011 |
| End | 07/2015 |
| Description | Training & Career Development Board - Research Bursary |
| Amount | £13,175 (GBP) |
| Organisation | Cancer Research UK |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 10/2014 |
| End | 11/2015 |
| Description | Analysing t-cell populations from patient blood after fish oil application on patients with pancreatitis |
| Organisation | Leicester Royal Infirmary |
| Department | Surgery |
| Country | United Kingdom |
| Sector | Hospitals |
| PI Contribution | Collaborating in clincal trial and providing expertise and infrastructure in laboratory with FACS machine |
| Collaborator Contribution | Design of clincal trial and patient enrollment. Contribution of time of research fellow to FACS analysis |
| Impact | Too early |
| Start Year | 2011 |
| Description | Apoptosis and cell cycle analysis after UVA,UVB induced cytotoxicity by selected drugs of Hut78 human T-cell lymphoma |
| Organisation | University of Leicester |
| Department | Department of Cancer Studies |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | advice on use of FACS and technical supervsion fo research |
| Collaborator Contribution | design of study |
| Impact | Too early |
| Start Year | 2011 |
| Description | Apoptosis and cell cycle analysis after UVA,UVB treatment of skin cells |
| Organisation | University of Leicester |
| Department | Department of Cancer Studies |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | support for use of FACS and guidance on appropriate use |
| Collaborator Contribution | design of project |
| Impact | Too early |
| Start Year | 2011 |
| Description | Apoptosis and cell cycle analysis of human melanoma and lymphoma |
| Organisation | University of Leicester |
| Department | Department of Cancer Studies |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | advice on use of FACS in programme and supervision of running of FACS sorting |
| Collaborator Contribution | Design of project |
| Impact | Too early |
| Start Year | 2010 |
| Description | Assessing expression levels of C1GaIT1, and its chaperone protein, IgA and non-IgA in B cells |
| Organisation | University of Leicester |
| Department | Department of Geology |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Support with experimental design and running the FACS sorter. |
| Collaborator Contribution | Study design, generation of samples and interpretation of data |
| Impact | None yet |
| Start Year | 2016 |
| Description | Blood analysis of fish oil treated patients with pancreatic cancer |
| Organisation | Leicester General Hospital |
| Department | Surgery and Pathology |
| Country | United Kingdom |
| Sector | Hospitals |
| PI Contribution | joint writing of prject protocol and supervision of FACS sorting. Patient recruitemnt |
| Collaborator Contribution | Joint protocol writing and patient recruitmetn |
| Impact | Too early |
| Start Year | 2011 |
| Description | Characterization of mesenchymal stem cells isolated from umbilical cord |
| Organisation | University of Leicester |
| Department | Department of Cardiovascular Sciences |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | contribution to research plan and running of FACS sorter |
| Collaborator Contribution | lead in design of research |
| Impact | Too early |
| Start Year | 2011 |
| Description | Determine mechanism of decrease in smooth muscle actin in fibrocytes when cocultured with ASM |
| Organisation | University of Leicester |
| Department | Department of Infection, Immunity and Inflammation |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | input to design of research project and running of FACS sorter |
| Collaborator Contribution | design of projest and colelction fo samples |
| Impact | Too early |
| Start Year | 2010 |
| Description | Developing Ang2 ligand trap by using Tie2 ectodomain mutants expressed in transfected DT40 cells. Sorting of mutants |
| Organisation | University of Leicester |
| Department | Department of Cardiovascular Sciences |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Practical support for us of FACS machine |
| Collaborator Contribution | Design of study and sample provision |
| Impact | Too early |
| Start Year | 2010 |
| Description | Directed evolution and structure/function analysis of proteins displayed on cell surface of B cells, DT40 and HEK293 cells |
| Organisation | University of Leicester |
| Department | Department of Cardiovascular Sciences |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Supervision of experiments using FACS sorter |
| Collaborator Contribution | Stduy design and provision of specimens |
| Impact | Manuscript in preparation |
| Start Year | 2010 |
| Description | Expression And Functional Significance Of The Endocannabinoid System In Human Spermatozoa |
| Organisation | University of Leicester |
| Department | Leicester Medical School |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | support to research group in use of FACS sorter and advice on optimal separation of stem cells |
| Collaborator Contribution | Has establsihed use of stem cell biology in endocannabinoid research and potential use in infertility |
| Impact | Has already led to presentation and abstract publication: "Expression And Functional Significance Of The Endocannabinoid System In Human Spermatozoa" FERTILITY AND STERILITY: Volume: 96 Issue: 3 Supplement: 1 Pages: S87-S87 Published: SEP 2011 This is multidisciplinary between Reproductive Sciences and Oncology and is to be extended to other istitutions in UK and abroad. |
| Start Year | 2010 |
| Description | Identification of specific cells types from lung that express KRAS-G12D by nasal Cre delivery. |
| Organisation | University of Leicester |
| Department | Department of Genetics |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Help with the isolation by FACS sorting of specific cell populations, through assistance with the experimental design and running of the instrument. |
| Collaborator Contribution | Design of the study, generating the samples, performing experiments and interpreting data. |
| Impact | None yet |
| Start Year | 2016 |
| Description | Identifying which cellular subtype is responsible for producing elevated concentrations of IFNalpha in PBMCs from COPD patients compared to controls |
| Organisation | University of Leicester |
| Department | Department of Infection, Immunity and Inflammation |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Study design and running of FACS sorter |
| Collaborator Contribution | Primary designers of research project and collection of samples |
| Impact | Too early |
| Start Year | 2010 |
| Description | Potential of Repurposed Drugs in Preventing Malignant Pleural Mesothelioma. |
| Organisation | University of Birmingham |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Conceived the idea and designed the project. Conducted the work and interpreted data. |
| Collaborator Contribution | Provision of a library of repurposed drugs and associated data. |
| Impact | Oral and poster conference presentations. |
| Start Year | 2015 |
| Description | Sorting airway epithelial stem cells from foetal human lung tissue stained with Hoechst 3342 |
| Organisation | University of Leicester |
| Department | Department of Infection, Immunity and Inflammation |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | use of FACS sorter and advice on protocol design |
| Collaborator Contribution | lead in designing ersearch prokject and collection specimens |
| Impact | Too early |
| Start Year | 2011 |
| Description | Sorting cancer stem cells from pancreatic tumors |
| Organisation | Leicester General Hospital |
| Department | Surgery and Pathology |
| Country | United Kingdom |
| Sector | Hospitals |
| PI Contribution | Support to train in use of FACS sorter and advice on optimal sorting techniques |
| Collaborator Contribution | Has extended models of use of stem cell sorting to pancreas |
| Impact | Is being used to rpedict response to therapy of pancreas cancer. Multidisciplinary with Pathology Department |
| Start Year | 2009 |
| Description | Sorting leucocyte rich plasma for nociceptin receptor for mRNA analysis |
| Organisation | Leicester Royal Infirmary |
| Department | Anaesthetics |
| Country | United Kingdom |
| Sector | Hospitals |
| PI Contribution | Advice and support throughout learning period of using Cell sorter. Contribution of technical support. |
| Collaborator Contribution | Will lead to joint publications and potential for joint grant application |
| Impact | Presentation at meeting and published abstract: Use of preparative fluorescence-activated cell sorting (FACS) to profile opioid receptor and peptide mRNA expression on human granulocytes, lymphocytes, and monocytes. Annual Meeting of the Anaesthetic-Research-Society Location: Univ Nottingham, Nottingham, ENGLAND: JUL 01-02, 2010 BRITISH JOURNAL OF ANAESTHESIA Volume: 105 Issue: 5 Pages: 707-708 Published: NOV 2010 This was multidisciplinary research with Anaesthetic department. |
| Start Year | 2009 |
| Description | Sorting to establish the stable transfection of clones |
| Organisation | University of Leicester |
| Department | Department of Infection, Immunity and Inflammation |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Support with experimental design and running the FACS sorter. |
| Collaborator Contribution | Study design, generation of samples and interpretation of data |
| Impact | None yet |
| Start Year | 2016 |
| Description | CRUK fundraisers annual meeting Leicester |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Participants in your research and patient groups |
| Results and Impact | 75 attendees at this talk on research in unit and large number of questions and discussion Visits to laboratories provided subsequently and requests for more talks. |
| Year(s) Of Engagement Activity | 2010 |
| Description | Local cancer fundraising group - Hope Leicester |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Participants in your research and patient groups |
| Results and Impact | Presentation to 60-80 attendees on use of stem cells in research and cancer prevention research. Many questions and extensive discussion. Invited back for next year as felt to be of great interest |
| Year(s) Of Engagement Activity | 2010,2011 |
| Description | School visit |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | Yes |
| Geographic Reach | Regional |
| Primary Audience | Schools |
| Results and Impact | 40 pupils attnded each event as part of University outreach intiative funded as part of GENIE programme. Talk on research in cacner prevention and role of stem cells in research. Many questions and considerable interest. High interest reported by school and organsiers fo events. |
| Year(s) Of Engagement Activity | 2010,2011 |
| Description | Science evening at Lutterworth High School |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | 50 schoolchildren and parents attended and sparked considerabel interest among both groups with questions and discussion Very well received by college staff who made the invitation |
| Year(s) Of Engagement Activity | 2011 |