Mechanisms of mRNA localisation and cytoskeletal transport

Lead Research Organisation: MRC Laboratory of Molecular Biology

Abstract

For cells to perform their elaborate functions, various components must be delivered to the right place at the right time. This is dependent on the cellular equivalent of a railway system; tiny machines called “molecular motors” transport “cargoes” to their destination by traversing a network of tracks within the cell. However, it is not clear how these motors recognise different cargoes and how these cargoes are sorted to different destinations. This is not only a fundamental problem in cell biology but is also relevant to the understanding and treatment of diseases; aberrant transport processes are associated with neurodegenerative illnesses and motors are frequently exploited by viruses and bacteria during infection.
We are studying the detailed mechanisms of motor-based transport in a genetically tractable model organism, the fruit fly Drosophila. In particular we have focused on how molecules of mRNA, which code for the proteins within cells, are trafficked to different destinations. By using advanced microscopy to visualise mRNA transport in living cells and reconstituted on glass slides, we can address the precise functions of specific proteins in motor transport. We also work directly on the roles of motors in neurons so we can begin to understand what goes wrong in several neuronal diseases.

Technical Summary

Cytoplasmic transport of organelles and macromolecules by molecular motors is of fundamental importance for the establishment and maintenance of cell polarity. In addition, defective motor transport is implicated in neurological diseases and pathogenic viruses and bacteria frequently exploit cellular transport routes. The challenge of studying microtubule-based transport is that movements are driven by large macromolecular assemblies, with a single cargo often bound simultaneously by multiple opposite polarity dynein and kinesin motors.
Using a tractable model system in Drosophila embryos we have shed light on mechanisms responsible for polarised sorting of specific mRNA molecules by microtubule motors. We have revealed molecular links between localising mRNAs and dynein and used novel in vitro motility assays to show that long distance movement of the motor is activated by mRNA localisation signals. Recently, together with Andrew Carter’s lab at the LMB, we have used purified mammalian proteins to show that the adaptor protein BicD is a key factor in switching on dynein processivity. We have also elucidated the mechanisms of dynein regulation by Lissencephaly-1 and mRNA localisation signals.
We have also been investigating how cargo transport is orchestrated in neurons using the intact Drosophila nervous system as a model. We recently established a novel imaging assay in the adult wing and are using it to study the relationship between axonal transport and the healthy lifespan of neurons. We have developed and distributed optimised tools for CRISPR genome engineering in Drosophila, and are using these in several of our own projects.
Our long-term plans are to combine genetics, cell biology, structural studies and single molecule assays to understand the key processes that control sorting of cargos and pathogens by motors. This should have therapeutic relevance; for example, it may be possible to target drugs to discrete regions of the cell to maximise their efficacy or to develop agents that stimulate motor activity in order to alleviate problems associated with defective transport in neurodegenerative diseases.

Publications

10 25 50
 
Description BBSRC working group on gene drive research
Geographic Reach Multiple continents/international 
Policy Influence Type Participation in a advisory committee
 
Description Centre for Science and Policy - Policy leaders forum
Geographic Reach National 
Policy Influence Type Participation in a advisory committee
 
Description Contributed to a policy forum piece recommending guidelines for safe use of gene drive technology in the laboratory
Geographic Reach Multiple continents/international 
Policy Influence Type Citation in other policy documents
Impact Cited by US National Academies of Science recommendations for guidelines on gene drive research.
URL https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4692367/
 
Description AstraZeneca/LMB Blue Skies Fund
Amount £200,000 (GBP)
Organisation AstraZeneca 
Sector Private
Country United Kingdom
Start 09/2018 
End 08/2020
 
Description Boehringer Ingelheim Fonds PhD Fellowships
Amount € 74,400 (EUR)
Organisation Boehringer Ingelheim 
Sector Private
Country Germany
Start 03/2013 
End 09/2016
 
Description DFG Research Fellowship (to JB)
Amount € 48,000 (EUR)
Organisation German Research Foundation 
Sector Public
Country Germany
Start 04/2016 
End 05/2019
 
Description EMBO Long-term Fellowship
Amount £54,000 (GBP)
Organisation European Molecular Biology Organisation 
Sector Learned Society
Country European Union (EU)
Start 01/2008 
End 12/2009
 
Description Lister Institute Outreach Award
Amount £2,000 (GBP)
Organisation Lister Institute of Preventive Medicine 
Sector Charity/Non Profit
Country United Kingdom
Start 02/2011 
End 02/2013
 
Description Lister Institute Summer Student Scheme
Amount £2,000 (GBP)
Organisation Lister Institute of Preventive Medicine 
Sector Charity/Non Profit
Country United Kingdom
Start 08/2014 
End 09/2014
 
Description Lister Institute Summer Studentship Scheme
Amount £2,000 (GBP)
Organisation Lister Institute of Preventive Medicine 
Sector Charity/Non Profit
Country United Kingdom
Start 06/2018 
End 08/2018
 
Description Lister Research Prize Fellowship (Mechanisms and neuronal functions of motor-driven transport)
Amount £200,000 (GBP)
Organisation Lister Institute of Preventive Medicine 
Sector Charity/Non Profit
Country United Kingdom
Start 10/2008 
End 09/2013
 
Description MRC Centenary Award
Amount £54,000 (GBP)
Organisation Medical Research Council (MRC) 
Sector Academic/University
Country United Kingdom
Start 09/2012 
End 04/2013
 
Description Marie Curie IEF
Amount £172,820 (GBP)
Organisation Marie Sklodowska-Curie Actions 
Sector Academic/University
Country Global
Start 07/2012 
End 06/2014
 
Description NC3Rs David Sainsbury Fellowship (for AV)
Amount £195,000 (GBP)
Organisation National Centre for the Replacement, Refinement and Reduction of Animals in Research (NC3Rs) 
Sector Private
Country United Kingdom
Start 07/2016 
End 06/2019
 
Description Swiss National Science Foundation
Amount £50,000 (GBP)
Organisation Swiss National Science Foundation 
Sector Public
Country Switzerland
Start  
 
Title Antibodies 
Description We have generated new antibodies to Drosophila proteins, which are available to other groups if they complete MTAs 
Type Of Material Antibody 
Provided To Others? No  
Impact We have used them for important experiments. We have not yet had requests from others. 
 
Title Fluorescent protein expressing flies 
Description We have developed several transgenic Drosophila (fruitfly) lines expressing fusion proteins with molecular motors and adaptor proteins fused to fluorescent proteins or affinity tags. These are available to the Drosophila community by request at which point MTA requirements are reviewed. These reagents should be of interest to several Drosophila groups studying mechanisms of neuronal function and dysfunction. 
Type Of Material Model of mechanisms or symptoms - non-mammalian in vivo 
Year Produced 2013 
Provided To Others? Yes  
Impact Results in a co-authored publication in 2013. 
 
Title Optimised CRISPR/Cas Drosophila tools 
Description We have generated transgenic flies expressing the enzyme Cas9 and plasmids for expression of gRNA. These tools can be used to efficiently create mutations in Drosophila. 
Type Of Material Model of mechanisms or symptoms - non-mammalian in vivo 
Year Produced 2013 
Provided To Others? Yes  
Impact The provision of the reagents will have a major impact on the research of many academic labs. We have already provided the tools to greater than 400 academic labs through the community stock centre and Addgene plasmid repository. They are being used to contribute to research advances in many areas of biomedical research. 
URL http://www.crisprflydesign.org
 
Title Particle tracking software 
Description A Mathematica code for tracking of fluorescent objects in cells with great precision 
Type Of Material Data analysis technique 
Year Produced 2006 
Provided To Others? Yes  
Impact Provided to other researcher in the MRC 
 
Description BICD2 and spinal muscular atrophy 
Organisation University College London
Country United Kingdom 
Sector Academic/University 
PI Contribution We provided structural analysis of human disease mutations in the BICD2 protein, an adaptor for the dynein motor, which cause inherited dominant spinal muscular atrophy with lower extremity dominances (SMA-LED).
Collaborator Contribution Our collaborators identified patients with BICD2 mutations and characterised their phenotypes. The collaboration provided new insights for our own mechanistic studies of the BICD protein family
Impact Manuscript published in highly valued journal Brain. Manuscript will help clinicians with diagnosis of SMA-LED and counseling of affected families. Multi-disciplinary study using clinical genetics, molecular biology and structural biology
Start Year 2013
 
Description CRISPR/Cas9 genome editing 
Organisation Howard Hughes Medical Institute
Country United States 
Sector Charity/Non Profit 
PI Contribution We have collaborated with the group to optimise the CRISPR/Cas9 genome editing system in Drosophila. We exchanged flies and results with the other group ahead of publication.
Collaborator Contribution We have collaborated with the group to optimise the CRISPR/Cas9 genome editing system in Drosophila. We exchanged flies and results with the other group ahead of publication.
Impact Manuscript published in PNAS describing our work, which is very highly cited. Licensing of transgenic flies generated to fly transgenic injection companies. Plasmids deposited at Addgene with over 500 requests to date
Start Year 2013
 
Description Link between ALS and dynein-based transport 
Organisation University of Leuven
Country Belgium 
Sector Academic/University 
PI Contribution We are testing models for disease mechanisms using in vitro motility assays
Collaborator Contribution Our partners are generating hypotheses with in vivo experiments
Impact Relatively new collaboration, so no publications yet. Collaboration involves mouse genetics, neuronal imaging (both in Belgium), biochemistry and single molecule imaging (my lab).
Start Year 2017
 
Description Mechanistic studies of dynein based transport 
Organisation Medical Research Council (MRC)
Department MRC Laboratory of Molecular Biology (LMB)
Country United Kingdom 
Sector Public 
PI Contribution We initially performed single molecule microscopy using proteins purified by our collaborators. We have since produced our own proteins to work on collaborative projects with single molecule microscopy.
Collaborator Contribution Production of recombinant and purified motor complex components. Structural analysis of dynein.
Impact Joint paper in EMBO Journal, which has been highly cited. Co-authors on a paper in PNAS, which has just been published.
Start Year 2013
 
Description Motor recruitment 
Organisation University of Bern
Department Institute of Cell Biology
Country Switzerland 
Sector Academic/University 
PI Contribution We have performed the majority of the experiments and designed the project
Collaborator Contribution Provided unpublished reagents used in the study
Impact The manuscript describing this work was published this year in Journal of Cell Biology
Start Year 2010
 
Description Motor recruitment 
Organisation Wellcome Trust
Department Wellcome Trust Centre for Cell Biology
Country United Kingdom 
Sector Charity/Non Profit 
PI Contribution We have performed the majority of the experiments and designed the project
Collaborator Contribution Provided unpublished reagents used in the study
Impact The manuscript describing this work was published this year in Journal of Cell Biology
Start Year 2010
 
Description Motor regulation 
Organisation University of California, Irvine
Department Department of Developmental and Cell Biology
Country United States 
Sector Academic/University 
PI Contribution N/A. The majority of the work was from our group.
Collaborator Contribution They helped in a minor way with data analysis for one of our papers.
Impact Their work contributed to the publication Bullock et al. 2006
 
Description Motors and synaptic vesicle recycling 
Organisation Iwaki Meisei University
Department Pharmacology Iwaki Meisei
Country Japan 
Sector Academic/University 
PI Contribution N/A. We were the major contributors to the project
Collaborator Contribution Their work on electrophysiology of neurons made important contributions to our paper Li et al. 2010.Their work helped towards a publication of ours
Impact Manuscript: Li et al. 2010
Start Year 2007
 
Description Motors and synaptic vesicle recycling 
Organisation University of York
Department Department of Biology
Country United Kingdom 
Sector Academic/University 
PI Contribution N/A. We were the major contributors to the project
Collaborator Contribution Their work on electrophysiology of neurons made important contributions to our paper Li et al. 2010.Their work helped towards a publication of ours
Impact Manuscript: Li et al. 2010
Start Year 2007
 
Description Regulation of dynein-mediated transport by a kinase 
Organisation University of Dundee
Country United Kingdom 
Sector Academic/University 
PI Contribution We performed RNA injections and analysis to characterise the effects of a mutation in a kinase on dynein-mediated mRNA transport
Collaborator Contribution Our partners are the major contributors to this project.
Impact A manuscript describing this work has been published in the journal Development.
Start Year 2012
 
Description Regulation of mRNA transport in vertebrate neurons 
Organisation University of Cambridge
Country United Kingdom 
Sector Academic/University 
PI Contribution We provided assistance in establishing a novel assay of mRNA transport in live Xenopus neurons and in the quantitative analysis of mRNA motility.
Collaborator Contribution Our partners are the major contributors to the project.
Impact A manuscript describing the work is currently being prepared for submission.
Start Year 2011
 
Description Regulation of mitochondrial transport during ageing 
Organisation University College London
Department Institute of Neurology
Country United Kingdom 
Sector Academic/University 
PI Contribution We have recently been awarded funding from the NCR3s for a bilateral project. This will start in July 2016. However, we are already collaborating on the groundwork for the project.
Collaborator Contribution Our partners will perform work in mouse models using their expertise in imaging mitochondrial transport
Impact The collaboration is now being pursued by my former post-doc who has set up his own laboratory. Multidisciplinary: cell biology, genetics, mouse models, Drosophila models, intravital imaging
Start Year 2016
 
Description Small molecules that alter microtubule-based transport 
Organisation AstraZeneca
Department Research and Development AstraZeneca
Country United Kingdom 
Sector Private 
PI Contribution We have established, together with Andrew Carter's lab at LMB, on a cellular assay for motor activity.
Collaborator Contribution The assay has been adapted by AZ and optimised for high content microscopy.
Impact Collaboration involves light microscopy, image analytics, chemistry
Start Year 2017
 
Description Structure of motor complex components 
Organisation John Innes Centre
Country United Kingdom 
Sector Charity/Non Profit 
PI Contribution We designed the project and performed all experiments
Collaborator Contribution Helped with interpretation of structural data
Impact Manuscript published in Genes and Development 2013.
Start Year 2011
 
Description Structure-function analysis of an RNA binding protein involved in mRNA localisation 
Organisation Max Planck Society
Department Max Planck Institute for Developmental Biology
Country Germany 
Sector Academic/University 
PI Contribution We are generating CRISPR genome edited flies to validate atomic structures generated by our collaborators. We will then characterise the phenotypes of these flies.
Collaborator Contribution Structural and biochemical analysis of the RNA binding protein
Impact Too early in the collaborative process. The work should advance our knowledge of RNA binding proteins in RNA localization.
Start Year 2016
 
Description dynein function in oskar mRNA localisation 
Organisation Georgia Health Sciences University
Country United States 
Sector Academic/University 
PI Contribution We provided transgenic flies for this study.
Collaborator Contribution Our partners were the major contributors to this project.
Impact A paper describing the work has been published in the journal PLoS One
Start Year 2013
 
Description oskar RNA localisation signal 
Organisation European Molecular Biology Laboratory
Country Germany 
Sector Public 
PI Contribution We provided substantial experimental work, which helped to map the dynein-dependent RNA localisation signal in the important mRNA transcript oskar.
Collaborator Contribution Our partners were the major contributors to the project.
Impact A manuscript describing the work has been published in the journal RNA.
Start Year 2011
 
Description piRNA pathways 
Organisation Skirball Institute of Biomolecular Medicine
Country United States 
Sector Academic/University 
PI Contribution We helped them in a project related to how motors are associated with retrotransposon silencing by performing technically challenging microinjection experiments.
Collaborator Contribution We helped them in a project related to how motors are associated with retrotransposon silencing. This has since has practical implications for our own research.
Impact Publication: Navarro, Bullock and Lehmann, PNAS, 2009
Start Year 2008
 
Title CRISPR tools for Drosophila research 
Description Optimised Cas9 endonuclease lines and gRNA expression plasmids for efficient genome engineering in Drosophila. Has been licensed to two commercial Drosophila injection companies. 
IP Reference  
Protection Protection not required
Year Protection Granted
Licensed Yes
Impact Will result in income for the MRC
 
Description 2019 LMB Science Image Award 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact I was a co-organiser of the LMB Science Image Award, which involves loaning microscopes to primary schools for their use. Schools can submit interesting images that the children have taken into our competition. Feedback from pupils and teachers was excellent, and we are running the event again this year. There was a write up of the activity in the Cambridge Independent newspaper.
Year(s) Of Engagement Activity 2019
URL https://www2.mrc-lmb.cam.ac.uk/the-winner-of-the-2018-lmbs-schools-science-image-award-is/
 
Description CRISPR fly design resource website 
Form Of Engagement Activity A magazine, newsletter or online publication
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Provides a major resource of information and protocols for CRISPR genome editing, particularly in Drosophila

Reputational benefits for my group and the LMB and MRC within the fly community. Lots of positive feedback at conferences.
Year(s) Of Engagement Activity 2013,2014,2015,2016,2017
URL http://www.crisprflydesign.org
 
Description Cambridge Science Festival 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? Yes
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Designed and organised the See your Cells activity at the Cambridge Science Festival, which involved several MRC volunteers.

Excellent feedback in the form of questionnaires submitted to the overall event organiser.
Year(s) Of Engagement Activity 2012,2013,2014,2015
URL http://www.cam.ac.uk/science-festival
 
Description Consulted for BBC programme on cell biology 
Form Of Engagement Activity A broadcast e.g. TV/radio/film/podcast (other than news/press)
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact I was interviewed on several occasions for a BBC programme on cell biology
Year(s) Of Engagement Activity 2013
 
Description Gene drive working group 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Policymakers/politicians
Results and Impact I am part of the BBSRC working group on gene drive research. We are tasked with providing recommendations to the BBSRC, which will help shape their formal policy document on gene drive research. Our report is still in progress.
Year(s) Of Engagement Activity 2018,2019
 
Description Interview with Guardian newspaper on Gene Drive technology and control of insect borne diseases 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact As an expert in CRISPR genome engineering I was interviewed by the Guardian about a study on gene drive based population control of mosquitoes. The piece featured on the front page of the Guardian and on the newspaper's website.
Year(s) Of Engagement Activity 2015
URL https://www.theguardian.com/science/2015/nov/23/anti-malarial-mosquitoes-created-using-controversial...
 
Description Interview with Sky news on CRISPR 
Form Of Engagement Activity A broadcast e.g. TV/radio/film/podcast (other than news/press)
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact We were visited by Sky news as part of their piece on CRISPR technology. We advised them on the piece and were interviewed on camera.
Year(s) Of Engagement Activity 2016
 
Description LMB Open Day 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact I was a co-organiser of this event which aimed to introduce the public to the work of the LMB. 2100 people visited. On the day I coordinated and participated in a set of tours of the different departments in the building.

Overwhelmingly positive feedback left on feedback forms. Stimulated interest from school children in a career in biosciences.
Year(s) Of Engagement Activity 2014
 
Description LMB Open day volunteer 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Member of SB's team was a volunteer on the Cell Biology tours at the 2017 LMB open day.
Year(s) Of Engagement Activity 2017
URL https://www2.mrc-lmb.cam.ac.uk/lmb-welcomes-2000-visitors-fun-packed-open-day/
 
Description LifeLab outreach activity (Cambridge) 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact One PhD student in my group helped run an exhibit on molecular motors.
Year(s) Of Engagement Activity 2018
URL https://www2.mrc-lmb.cam.ac.uk/an-engaging-month-for-lmb-researchers/
 
Description LifeLab outreach activity (Peterborough) 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Our 'See Your Cells' exhibit was part of the LifeLab (European Researchers' Night) set of activities in Queensgate Shopping Centre, Peterborough. We had many discussions with members of the public on MRC-funded research, as well as allowing them to participate in a fun microscopy-based activity.
Year(s) Of Engagement Activity 2019
URL https://www2.mrc-lmb.cam.ac.uk/an-engaging-month-for-lmb-researchers/
 
Description Microscope4Schools project 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Schools
Results and Impact Together with a post-doc in the division I initiated a project to take educational microscopes into primary schools to educate them and enthuse them about science and the work of the MRC. This project, which has been very well received, also included the development of a website (http://www2.mrc-lmb.cam.ac.uk/microscopes4schools/) allowing teacher, parents and pupils worldwide to learn how to buy and use cheap educational microscopes. We typically get approximately 300 visits to the website per day. We have had visits to the website from 163 countries. Others, including the Gurdon Institute and CIMR, have set up similar activities after witnessing our programme.

Excellent feedback questionnaires from children. In some cases claiming that they now want to be a scientist when they grow up etc. That they understand that a goal of the MRC is to make discoveries that lead to new medicines or diagnostic tools.
Year(s) Of Engagement Activity 2011,2012,2013,2014,2015
URL http://www2.mrc-lmb.cam.ac.uk/microscopes4schools/
 
Description One student composed music for LMB "Home in the Service of Science" artist in residence performance piece. Another student performed in the piece. 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact LMB Home in the Service of science performance piece
Year(s) Of Engagement Activity 2016
URL http://www2.mrc-lmb.cam.ac.uk/homeinsci/
 
Description Organisation of LMB open day 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Organised tours of the Cell Biology division during the open day.
Year(s) Of Engagement Activity 2017
URL https://www2.mrc-lmb.cam.ac.uk/lmb-welcomes-2000-visitors-fun-packed-open-day/
 
Description Participation in policy forum with senior civil servants on genetic technologies "Policy leaders forum". 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Policymakers/politicians
Results and Impact Organised by Centre for Science and Policy, this was a gathering of experts in genetic technologies and senior civil servants from the UK government. Aim was to brief attendees on emerging genetic technologies and discuss commercial opportunities, biosafety issues and public engagement.
Year(s) Of Engagement Activity 2017
 
Description Science Image Award Competition for Schools 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Schools
Results and Impact I co-organise this activity, which involves lending microscopes to local schools so that they can image the microscopic world in their locality. The schools enter their best images into an annual competition. The feedback is excellent: teachers report that children become enthused about the plants and animals in their gardens or school.
Year(s) Of Engagement Activity 2018
URL https://www2.mrc-lmb.cam.ac.uk/microscopes4schools/award.php
 
Description Scientific presentation to Chairman of Wellcome Trust 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Policymakers/politicians
Results and Impact Presentation of one of 4 LMB scientists on ongoing research to Sir William Castell

Positive feedback to LMB deputy director
Year(s) Of Engagement Activity 2009
 
Description Scientific presentations to DIUS 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Policymakers/politicians
Results and Impact One of three LMB scientists presenting primary research to members of DIUS as part of visits to assess new building proposals

Positive feedback from DIUS on LMB science
Year(s) Of Engagement Activity 2007,2008
 
Description See Your Cells at 2017 Cambridge Science Festival 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact LMB exhibit that aims to educate children about the microscopic world and the importance of research into it
Year(s) Of Engagement Activity 2017
 
Description Seminar to LMB non-scientific staff 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact 60 non-scientific staff attended as part of the LMB program to engage them with research in the institute

Was asked to present talk to MRC Ares mouse staff
Year(s) Of Engagement Activity 2011
 
Description Seminar to MRC ARES mouse house staff 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact 30 mouse house staff attended

Positive feedback
Year(s) Of Engagement Activity 2011
 
Description Subject of a piece in the Cambridge Independent newspaper about work on cargo transport within cells 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Media (as a channel to the public)
Results and Impact I was interviewed by the newspaper, which resulted in a two page article highlighting the work of my group and a collaborator at the LMB. The piece also covered our outreach project, Microscopes4Schools
Year(s) Of Engagement Activity 2017
URL http://www.cambridgeindependent.co.uk/business/science/how-cambridge-scientists-are-exploring-the-in...