Lipid Profiling and Signalling: Macronutrients and Metabolic Health

Lead Research Organisation: MRC Human Nutrition Research Group

Abstract

According to statistics reported by Diabetes UK in 2010, since 1996 the number of people diagnosed with diabetes has increased from 1.4 million to 2.6 million, and is set to rise to four million people by 2025. The majority of these cases are associated with type 2 diabetes (T2DM), with the increased prevalence arising from an increasingly aged population and the rise in overweight and obese people. The interaction between being overweight and T2DM arises because fats become inappropriately stored in organs such as the liver, pancreas and skeletal muscle, reducing the body’s capacity to respond to the hormone insulin, an important signal which regulates the storage of nutrients. However, one central enigma concerning the interaction between obesity and T2DM is why some individuals appear to have a near limitless capacity for storing fat without proceeding to diabetes, while other appear to be pre-disposed to the disease.
The lipid profiling and signalling group are using novel mass spectrometry, nuclear magnetic resonance spectroscopy and computer aided modelling to understand this problem. We have a range of studies that investigate the effects of over-nutrition, and in particular the role that high fat intake plays in inducing disease. The benefits of this work are an improvement in our understanding of the disease process, providing new drug targets, and the identification of ‘biomarkers’, molecular signatures of disease or treatment that could be used for diagnostics.

Technical Summary

Understanding how fat metabolism is regulated in the human body is essential to understanding a number of major pathologies affecting human health including type II diabetes (T2DM), obesity, atherosclerosis and fatty liver disease. The incidence of both T2DM and obesity are rising rapidly across the globe as a result of over nutrition. The aim of the Lipid Profiling and Signalling programme is to understand why on an individual basis certain people develop insulin resistance and subsequently type 2 diabetes, while others stay metabolically healthy, and in particular how diet interacts with relative risk. We investigate the regulation of lipid metabolism and its interplay with health and diseases of over nutrition using a combination of state of the art mass spectrometry, NMR spectroscopy and bioinformatics to use an individual’s metabolism as a measure of their relative risk of developing disease. The programme consists of four inter-related areas of research.
The first involves the understanding of adipose tissue metabolism in health and disease. In particular through work on peroxisome proliferator activated receptors (PPARs), three receptors that play a central role in regulating metabolism at the whole organism level, we have investigated the regulation between lipid storage and fatty acid oxidation in this tissue. This has led us into work understanding small molecule activators of mitochondrial biogenesis in white adipose tissue, so called ‘browning’ agents, which could be used to reduce obesity and restore metabolic health. In addition we have developed a quantitative method for the profiling of lipid mediators, including over 100 eicosanoids and related metabolites. These mediators are important in understanding some of the mechanisms thought to mediate insulin resistance and in particular how the generation of reactive oxygen species produce lipid mediators linked to inflammation of key organs including adipose tissue.
The second area of research involves the consequences of ectopic fat deposition in liver and skeletal muscle, and how this influences insulin resistance. In addition to work in global lipidomics and eicosanoid profiling detailed above, we have also developed methods to examine how changes in fat metabolism affect the composition of sub-cellular organelles and cellular function, such as inducing endoplasmic reticulum stress and mitochondrial dysfunction. We are developing mass spectrometry tools to profile the lipid composition of sub-cellular membranes in order to understand how changes in lipid metabolism alter sub-cellular function and hence influence disease at a cellular level.
The third area of research is in molecular epidemiology. To assess the impact diet has on T2DM and the metabolic syndrome, we, in conjunction with members of MRC Epidemiology and the Institute of Public Health, University of Cambridge, are using lipidomics and metabolomics to profile individuals at the epidemiological scale and cross correlate changes in metabolism with anthropometric and genome wide association studies.
All this work is strongly underpinned by bioinformatics and multivariate statistics. We have developed a range of bioinformatic tools for the processing and storage of lipidomic data. These tools are currently being used in-house in a number of collaborative lipidomic studies into aspects of the metabolic syndrome including large scale epidemiology studies and mass spectrometry imaging of fatty liver disease.

Organisations

Publications

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Acharjee A (2017) The translation of lipid profiles to nutritional biomarkers in the study of infant metabolism. in Metabolomics : Official journal of the Metabolomic Society

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Ament Z (2013) A metabolomics investigation of non-genotoxic carcinogenicity in the rat. in Journal of proteome research

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Ament Z (2016) Metabolomics dataset of PPAR-pan treated rat liver. in Data in brief

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Ashmore T (2015) Suppression of erythropoiesis by dietary nitrate. in FASEB journal : official publication of the Federation of American Societies for Experimental Biology

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Atzori L (2017) How to bridge metabolomics and genomics. in The international journal of biochemistry & cell biology

 
Description SACN advisory committee on dietary fats
Geographic Reach National 
Policy Influence Type Participation in a advisory committee
 
Description Amegen Summer studentship - Xavier Fernandez
Amount £3,000 (GBP)
Organisation Amgen Inc 
Sector Private
Country United States
Start 06/2015 
End 08/2015
 
Description Astra Zeneca CASE studentship
Amount £40,000 (GBP)
Organisation AstraZeneca 
Sector Private
Country United Kingdom
Start 10/2016 
End 09/2019
 
Description BBSRC Industrial CASE award
Amount £18,000 (GBP)
Organisation Selcia 
Sector Private
Country United Kingdom
Start 10/2011 
End 09/2014
 
Description BBSRC Industrial CASE award
Amount £65,000 (GBP)
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 10/2011 
End 09/2014
 
Description BBSRC project grant - Burning fat, understanding the role of PPAR delta in adipose tissue
Amount £485,000 (GBP)
Funding ID BB/H013539/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 12/2010 
End 11/2013
 
Description BBSRC responsive mode grant
Amount £864,000 (GBP)
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 07/2014 
End 06/2017
 
Description BHF Intermediate fellowship for KT
Amount £250,000 (GBP)
Organisation British Heart Foundation (BHF) 
Sector Charity/Non Profit
Country United Kingdom
Start 10/2016 
End 09/2019
 
Description British Heart Foundation PhD Studentship
Amount £110,442 (GBP)
Organisation British Heart Foundation (BHF) 
Sector Charity/Non Profit
Country United Kingdom
Start 10/2012 
End 09/2015
 
Description COSMOS
Amount € 76,184 (EUR)
Organisation European Commission 
Department Seventh Framework Programme (FP7)
Sector Public
Country European Union (EU)
Start 11/2012 
End 10/2015
 
Description DRINC - Validation of Biomarkers of efficacy for fetal nutrition
Amount £550,000 (GBP)
Funding ID BB/M027252/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 10/2015 
End 09/2018
 
Description EPSRC Industrial Studentship
Amount £25,000 (GBP)
Organisation Koninklijke Philips Electronics N.V. 
Department Philips
Sector Private
Country Global
Start 10/2011 
End 09/2016
 
Description EPSRC Industrial Studentship
Amount £65,000 (GBP)
Organisation Engineering and Physical Sciences Research Council (EPSRC) 
Sector Academic/University
Country United Kingdom
Start 10/2011 
End 09/2016
 
Description ERC Senior Investigator Awaard - EPIC Heart
Amount € 225,000 (EUR)
Organisation European Research Council (ERC) 
Sector Public
Country European Union (EU)
Start 09/2013 
End 08/2017
 
Description EU FP7 EPIC CVD
Amount € 360,000 (EUR)
Organisation European Commission 
Department Seventh Framework Programme (FP7)
Sector Public
Country European Union (EU)
Start 10/2013 
End 09/2014
 
Description GSK-Astra Zeneca partnership awards
Amount £417,000 (GBP)
Organisation GlaxoSmithKline (GSK) 
Sector Private
Country Global
Start 04/2017 
End 03/2020
 
Description GlaxoSmithKline conversion of a studentship to a CASE award
Amount £22,000 (GBP)
Organisation GlaxoSmithKline (GSK) 
Sector Private
Country Global
Start 10/2011 
End 09/2013
 
Description High-throughput "omic" science and imaging call
Amount £1,300,000 (GBP)
Organisation Medical Research Council (MRC) 
Sector Academic/University
Country United Kingdom
Start 07/2013 
End 07/2014
 
Description MRC CASE studentship
Amount £18,000 (GBP)
Organisation Unilever 
Sector Private
Country United Kingdom
Start 09/2011 
End 09/2014
 
Description MRC CASE studentship
Amount £65,000 (GBP)
Organisation Medical Research Council (MRC) 
Sector Academic/University
Country United Kingdom
Start 10/2011 
End 09/2014
 
Description MRC Centenary Award NB
Amount £33,000 (GBP)
Organisation Medical Research Council (MRC) 
Sector Academic/University
Country United Kingdom
Start 10/2012 
End 09/2013
 
Description Marie Curie Mobility Fellowship
Amount € 60,000 (EUR)
Funding ID Not known 
Organisation Marie Sklodowska-Curie Actions 
Sector Academic/University
Country Global
Start 09/2015 
End 09/2016
 
Description Medical Research Council Bioinformatics Structure
Amount £11,679,000 (GBP)
Organisation Medical Research Council (MRC) 
Sector Academic/University
Country United Kingdom
Start 07/2014 
End 06/2017
 
Description Medimmune industrial studentship
Amount £100,000 (GBP)
Organisation MedImmune 
Department MedImmune Cambridge
Sector Private
Country United Kingdom
Start 10/2016 
End 09/2019
 
Description Metabolights: Creating the missing metabolomics community database resource.
Amount £245,000 (GBP)
Funding ID BB/I000933/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 10/2010 
End 09/2013
 
Description NIH-Cambridge Studentship in translational medicine
Amount £100,000 (GBP)
Organisation National Institutes of Health (NIH) 
Sector Public
Country United States
Start 10/2017 
End 09/2020
 
Description Optimized Metabolite Extraction, Separation, and Identification for Metabolomics
Amount £420,179 (GBP)
Funding ID ES022186-04 
Organisation National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) 
Sector Public
Country United States
Start 05/2013 
End 04/2017
 
Description Technology Development Grant, MetaboFlow - the development of standardised workflows for processing metabolomics data to aid reproducible data sharing and big data initiatives
Amount £900,000 (GBP)
Funding ID 202952/B/16/Z 
Organisation Wellcome Trust 
Sector Charity/Non Profit
Country United Kingdom
Start 12/2016 
End 11/2019
 
Description Unilever CASE funded studentship
Amount £40,000 (GBP)
Funding ID NA 
Organisation Unilever 
Department Unilever Research and Development
Sector Private
Country United Kingdom
Start 10/2017 
End 09/2020
 
Description Wellcome Trust and GSK studentship in translational medicine
Amount £65,000 (GBP)
Organisation Wellcome Trust 
Sector Charity/Non Profit
Country United Kingdom
Start 10/2013 
End 09/2016
 
Title Caldera 
Description HNR's bioinformatics team has developed a centralised data processing platform facilitating the reproducible analysis of large-scale studies carried out on high-resolution mass-spectrometry instruments. 
Type Of Material Improvements to research infrastructure 
Provided To Others? No  
Impact Currently, a wide range of different instruments types are used in the lipidomics community with instrument manufacturers specifying proprietary file formats and supplying their own analysis software. Most of these commercially available software packages are designed as closed source systems, so details on how algorithms are implemented and how parameters are specified remains unclear to the end-user. In addition, commercial software packages are often not designed in a scalable way, i.e. they are not able to handle extremely large sample cohorts. These problems often result in a lack of reproducibility and transparency which makes it very difficult to compare results across different labs and thus can be regarded as one of the biggest challenges facing the whole community. To overcome this problem HNR's bioinformatics team has developed a data processing platform, which relies on open standards and open software. The system is workflow-based and each step of data processing is transparent to the end-user. All settings used can be predefined, facilitating the reproducible analysis of any given data set at any given point in time. In addition, parameter files describing the settings for complex operations (for example from peak picking in spectral raw data to statistical analysis) can be easily run by non-experts ensuring optimal results can be achieved any time. Furthermore, parameters sets and workflows can easily be shared amongst researchers working in different labs. Within LPS, Caldera serves as the central framework for data processing, and is successfully used in context of a large variety of studies conducted on different instrument types. 
 
Title CompDB 
Description For the annotation of spectral peaks HNR's bioinformatics team has developed an in-house compound database containing several thousand lipid species. 
Type Of Material Biological samples 
Provided To Others? No  
Impact Lipidomics data, especially when generated using high-resolution mass spectrometry instruments, are of a complex nature and can easily contain thousands of individual spectral features. The annotation of these features (so called peaks) is crucial for the interpretation of data in the biological context. CompDB is specifically tailored to work with complex input data and can handle large queries in in a fast and automated way, which cannot be achieved using openly available web-based compound databases. Furthermore, it also provides more flexibility for the user - it (for example) allows the automated search for potential putative adduct formations that are observed frequently in "soft ionisation" techniques and are often difficult and very time-consuming to detect in a manual database searches. In addition, CompDB keeps track of successfully annotated peaks as well as of so called "Unknowns" making it a valuable source of information for future experiments or for the characterisation of specific sample types. Within LPS, CompDB serves as a central starting point for the non-ambiguous identification of compounds and thus is crucial for all on-going lipidomics research at HNR. The database design is scalable, ensuring that it is fit for future demands. 
 
Description Abu Dhabi Diabetes Centre Abu Dhabi, United Arab Emirtes 
Organisation Imperial College London Diabetes Centre
Country United Arab Emirates 
Sector Hospitals 
PI Contribution LPS has been approached by the Abu Dhabi Diabetes Centre Abu Dhabi, United Arab Emirtes, about setting up a collaboration in type 2 diabetes. This is an interesting proposal as the Gulf contains the highest incidence of type 2 diabetes in the world and they are currently performing genome wide association studies. Some metabolomics would be performed in house (amino acid analysis) but UAE are interested in running the intact lipids at MRC HNR.
Collaborator Contribution At the moment HNR is largely providing information and assistance.
Impact None to date
Start Year 2012
 
Description BHF programme grant on BAT 
Organisation Medical Research Council (MRC)
Department MRC Centre for Obesity and Related Metabolic Diseases
Country United Kingdom 
Sector Public 
PI Contribution Collaboration on a programme grant application to the British Heart Foundation. This has costs for lipidomics to profile eicosanoids and intact lipids in order to understand the role brown adipose tissue may play in treating atherosclerosis.
Collaborator Contribution The partner is doing the majority of the animal work and molecular biology for this project.
Impact None to date
Start Year 2012
 
Description Biocrates Collaboration on Bile Acid Analysis 
Organisation Biocrates Life Sciences
Country Austria 
Sector Private 
PI Contribution A plate to measure 17 bile acids for 40 samples (mainly liver homogenates but can include plasma or other matrices) has been prepared by the company BIOCRATES. They have offered a 96 well plate for performing this analysis to LPS in return for LPS evaluating the performance of the method. BIOCRATES wanted the data back by the end of July. Elizabeth Stanley identified a suitable set of samples for the trial.
Collaborator Contribution TBC
Impact TBC
Start Year 2014
 
Description Cambridge Metabolism Network 
Organisation AstraZeneca
Country United Kingdom 
Sector Private 
PI Contribution I am a member of the organising committee for the Cambridge Metabolomics Network. This has included two meetings on metabolism. The events have been sponsored by AZ, MedImmune and Novartis and contact with AZ led to a CASE pHD studentship.
Collaborator Contribution To my group they have provided a PhD studentship.
Impact Got a CASE studentship in a multi-disciplinary research area.
Start Year 2015
 
Description Cambridge Metabolism Network 
Organisation MedImmune
Country United States 
Sector Private 
PI Contribution I am a member of the organising committee for the Cambridge Metabolomics Network. This has included two meetings on metabolism. The events have been sponsored by AZ, MedImmune and Novartis and contact with AZ led to a CASE pHD studentship.
Collaborator Contribution To my group they have provided a PhD studentship.
Impact Got a CASE studentship in a multi-disciplinary research area.
Start Year 2015
 
Description Collaboration on hypertrophic cardiomyopathy. 
Organisation Oxford University Hospitals NHS Foundation Trust
Country United Kingdom 
Sector Public 
PI Contribution LPS has set up a collaboration to perform metabolomics and lipidomics on a study of cardiomyopathy patients with the John Radcliffe Hospital, University of Oxford. This would involve analysing blood plasma across time points from individuals with diabetes, dilated cardiomyopathy, hypertrophic cardiomyopathy and hypertension.
Collaborator Contribution They are providing the animal models and human tissue.
Impact None to date
Start Year 2012
 
Description Collaboration with Academic Rheumatology, King's College London 
Organisation King's College Hospital Charity
Country United Kingdom 
Sector Charity/Non Profit 
PI Contribution I agreed to collaborate on a grant examining the role of the mevalonate pathway in the immune system. While we will receive costs for the analyses performed this is also of interest as the immune system induced inflammation is a major cause of insulin resistance.
Collaborator Contribution See above
Impact Grant application by King's College
Start Year 2014
 
Description Collaboration with Biocrates to develop a kit to measure bile acids 
Organisation Biocrates Life Sciences
Country Austria 
Sector Private 
PI Contribution We have collaborated with Biocrates, an Austrian company that develops kits for metabolomics. We have examined a Bile acid beta test kit for use with liver tissue.
Collaborator Contribution Provided a kit they wanted us to test for bile acid measurements
Impact We provided feedback on the kit.
Start Year 2015
 
Description Collaboration with CIPH to look at INTERVAL 
Organisation University of Cambridge
Department Cambridge Institute of Public Health
Country United Kingdom 
Sector Academic/University 
PI Contribution Prof John Danesh (IoPH) approached Jules Griffin to set up a meeting to discuss collaborations and in particular a new venture to possibly look at INTERVAL, a 50,000-person study of very well-characterised healthy people across England in whom genomic measurements are being completed.
Collaborator Contribution TBC
Impact TBC
Start Year 2014
 
Description Collaboration with Centre for Public Health Research, Kenya Medical Research Institute, Nairobi, Kenya to set up a Lipids & Health Research group at the KEMRI 
Organisation Kenyan Institute for Medical Research (KEMRI)
Country Kenya 
Sector Public 
PI Contribution The Centre for Public Health Research, Kenya Medical Research Institute (KEMRI), Nairobi, Kenya to help with the set-up of a lipids and health research group at the KEMRI.
Collaborator Contribution KEMRI will provide samples to analyse by LPS and will send scientists to be trained by LPS
Impact project is on-going
Start Year 2014
 
Description Collaboration with Department of Biochemistry (RF) 
Organisation University of Cambridge
Department Department of Biochemistry
Country United Kingdom 
Sector Academic/University 
PI Contribution We are studying how lipids influence the role of collagen in platelet activation. In addition we are also collaborating with Prof. Wolfgang Seiss at Munich university.
Collaborator Contribution There are two grant applications under review - one to the British heart Foundation and the other to the Medical research Council
Impact None as yet.
Start Year 2011
 
Description Collaboration with Department of Epidemiology and Prevention, IRCCS Istituto Neurologico Mediterraneo Neuromed, Italy re Lipidomics & Poliphemus 
Organisation Institute for Cancer Research
Country Italy 
Sector Charity/Non Profit 
PI Contribution Michele Vacca and Jules Griffin approached Michele's former group to establish a collaboration for studying serum metabolomics in healthy subjects and patients with Metabolic Syndrome before and after nutritional interventions.
Collaborator Contribution Licia Iacoviello, MD, PhD, Head, Laboratory of Molecular and Nutritional Epidemiology
Impact TBC
Start Year 2014
 
Description Collaboration with Dr Stéphane Hazebrouck (Laboratoire INRA d'Immuno-Allergie Alimentaire, Service de Pharmacologie et d'Immunoanalyse) & Dr Anne-judith Waligora-Dupriet (Université Paris Descartes) 
Organisation French National Institute of Agricultural Research
Country France 
Sector Public 
PI Contribution Collaboration with Laboratoire INRA d'Immuno-Allergie Alimentaire, Service de Pharmacologie et d'Immunoanalyse, Gif sur Yvette and Université Paris Descartes to study the role of of the diet and gut microflora on the odd-chain fatty acid levels. Both labs have samples available. The odd-chain fatty acid containing lipids in these samples was analysed.
Collaborator Contribution These groups provide samples
Impact Practical work is on-going
Start Year 2014
 
Description Collaboration with Medimmune 
Organisation MedImmune
Country United States 
Sector Private 
PI Contribution We have initated a collaboration with medimmune in terms of making our expertise and facilities in metabolomics and lipidomics available to the cardiovascular and metabolic diseases group and in return we have been offered access to their high throughput cell culture facilities, we are discussing CASE studentships and also access to previously generated mouse models of obesity and fatty liver disease.
Collaborator Contribution See above!
Impact None as yet
Start Year 2014
 
Description Collaboration with Transplantation Medicine, Addenbrooke's Hospital on the development of VOCs to detect Acute Kidney Injury 
Organisation Addenbrooke's Hospital
Country United Kingdom 
Sector Hospitals 
PI Contribution Collaboration with Clatworthy group (Transplantion Medicine, Addenbrooke's & MRC LMB) on the development of volatile organic compounds (VOCs) in urine to detect acute kidney injury (AKI). Their aim is to use electrochemical or spectroscopic methods that could be used at the bedside. They want to use GC to validate their work and we are discussing headspace analysis to determine the VOCs of healthy people versus AKI patients. They offered to purchase the additional equipment to do headspace SPME on the Gerstel MPX systems. HNR will profile the VOCs of different patient groups.
Collaborator Contribution The Clatworthy will provide samples
Impact work is on going
Start Year 2014
 
Description Collaboration with pathology, University of Cambridge to examine the metabolic effects of viral growth in cell culture 
Organisation University of Cambridge
Country United Kingdom 
Sector Academic/University 
PI Contribution We had a collaboration to look into the effects of viral growth in human cells on cell metabolism using NMR spectroscopy. This has resulted in a publication.
Collaborator Contribution See abive
Impact Publication
Start Year 2014
 
Description Collaboration with the Department of Physiology Development & Neuroscience, University of Cambridge (HNR project 6503) 
Organisation University of Cambridge
Department Department of Physiology, Development and Neuroscience
Country United Kingdom 
Sector Academic/University 
PI Contribution Liver metabolome (HNR project 6503), liver, plasma, adipose and intestine samples were collected for a mouse strain and gender comparison of the lipid and low molecular weight species of the liver metabolome (many thanks to JLG, Xinzhu Wang, LR and Frank Sanders for the sample collection.
Collaborator Contribution Andrew Murray's group in the Department of Physiology Development and Neuroscience, UCam oversaw the study
Impact TBC
Start Year 2014
 
Description Collaboration with the Gurdon institute to follow fat metabolism in C.elegans 
Organisation University of Cambridge
Department Gurdon Institute
Country United Kingdom 
Sector Academic/University 
PI Contribution We are developing high throughput lipidomic methods for profiling fat metabolism in C.elegans. the aim is to understand the genetic regulation of fat metabolism at the whole genome level.
Collaborator Contribution We have produced a manuscript and are currently writing another.
Impact This is an on-going collaboration of JG which is now part of this new programme. It has previously brought in funding from the EU under a Marie Curie fellow and has produced two applications to the BBSRC for further funding.
Start Year 2011
 
Description Collaboration with university of Cagliari 
Organisation University of Cagliari
Department Department of Toxicology
Country Italy 
Sector Academic/University 
PI Contribution The University of Cagliari (department of Toxicology and Pathology) and LPS initiated a collaboration examining fatty liver disease by lipidomics and mass spectrometry imaging. The project is based around drug- induced fatty liver disease and how this develops into liver cancer. Cagliari would perform the animal studies and histology and LPS would provide lipidomic analysis.
Collaborator Contribution Generation of animal model and providing tissue samples
Impact A member of LPS was made a visiting professor of the university and we hosted a visiting student.
Start Year 2012
 
Description EPIC HEART 
Organisation University of Cambridge
Department Cardiovascular Epidemiology Unit
Country United Kingdom 
Sector Academic/University 
PI Contribution The UNIVERSITY, specifically the Cardiovascular Epidemiology Unit in the Department of Public Health and Primary Care, has plasma aliquots from CLBRI has agreed to perform fatty acid analysis of the 12,000 coronary disease cases ("Sample(s)") from the EPIC-Heart Study. CLBRI will analyse plasma aliquots by GC-FID for a panel of up to 38 fatty acids in exactly the same manner as has been previously used for Type 2 diabetes cases and controls from the EPIC InterAct study.
Collaborator Contribution They will provide the samples and will jointly analyse the data
Impact not yet
Start Year 2012
 
Description EPIC-CVD 
Organisation University of Cambridge
Department Cambridge Institute of Public Health
Country United Kingdom 
Sector Academic/University 
PI Contribution The UNIVERSITY, specifically the Cardiovascular Epidemiology Unit in the Department of Public Health and Primary Care, has plasma aliquots from CLBRI has agreed to perform fatty acid analysis of the 12,000 stroke ("Sample(s)") from the EPIC-CVD Study. CLBRI will analyse plasma aliquots by GC-FID for a panel of up to 38 fatty acids in exactly the same manner as has been previously used for Type 2 diabetes cases and controls from the EPIC InterAct study.
Collaborator Contribution provide samples and joint data analysis
Impact sample analysis is progress not output yet
Start Year 2013
 
Description Fenland 
Organisation University of Cambridge
Department MRC Epidemiology Unit
Country United Kingdom 
Sector Multiple 
PI Contribution We have been collaborating with MRC Epidemiology to profile amino acid metabolism in blood plasma from the Fenland study. We have undertook to expand this analysis to now include intact lipids on 1500 people with genotype information.
Collaborator Contribution MRC Epidemiology have collected the samples and genotyped individuals.
Impact We applied for and were awarded £1.3 million grant to conduct a joint GWAS and metabolomic study in collaboration with MRC epidemiology.
Start Year 2011
 
Description GLUE grant 
Organisation University of Pennsylvania
Department Institute for Translational Medicine and Therapeutics (ITMAT)
Country United States 
Sector Academic/University 
PI Contribution We are part of the analytical core to carry out metabolomics and lipidomics for an NIH GLUE grant into personalised medicine. This grant has been deemed fundable and we are awaiting approval of funding (which is linked to the US governments spending review).
Collaborator Contribution Its allowed a number of bilateral collaborations to be formed in addition to the large GLUE grant.
Impact This has led to a five year funded grant to support the work done in conjunction with the university of Cambridge.
Start Year 2011
 
Description GlaxoSmithKline collaboration in lipidomics 
Organisation GlaxoSmithKline (GSK)
Department Safety Assessment GSK
Country United Kingdom 
Sector Private 
PI Contribution We have assisted GSK in a number of projects centred around lipidomics and metabolomics, both in diabetes and toxicology research. The most recent area has been in understanding phospholipidosis, a problem of a number of widely administered drugs, and its relevance to fatty organ disease (particularly the liver, but also fat infiltration into lungs and brain tissue) and lysosomal storage disorders.
Collaborator Contribution They will make their laboratories open to the student and have also provided consumables for the student.
Impact This has led to the CASE component being funded for an MRC ITTP studentship in the LPS group.
Start Year 2011
 
Description Lipid profiling of Cambrdge Baby Growth Study Dried Blood Spot samples 
Organisation University of Cambridge
Department Department of Paediatrics
Country United Kingdom 
Sector Academic/University 
PI Contribution CLBRI will develop and validate a method for the lipid profiling of dried blood spots on paper, based on 2.4mm diameter blood spots collected during the Cambridge Baby Browth Study. After successful development and validation, CLBRI will analyse a pilot set (80 samples) and the full sample set (ca 800 samples) available from the Cambridhe Baby Growth Study.
Collaborator Contribution Provide the samples, meta-data and cover cost per sample (£12.50 per sample). The obtained data will be analysed jointly.
Impact No output yet
Start Year 2012
 
Description Lipidomics Analysis of The Interval Study 
Organisation University of Cambridge
Department Cambridge Institute of Public Health
Country United Kingdom 
Sector Academic/University 
PI Contribution The INTERVAL study involves the analysis of 50000 blood plasma samples from individuals giving blood and cross correlating this information with GWAS data collected by IPH. HNR will receive £250,000 for this work.
Collaborator Contribution GWAS analysis of 50000 people.
Impact To come.
Start Year 2015
 
Description Lipidomics in Cambridge 
Organisation Babraham Institute
Country United Kingdom 
Sector Private 
PI Contribution We are collaborating to provide a comprehensive lipidomic service for academic and pharma in the cambridge area.
Collaborator Contribution This partnership will raise the profile of lipidomics in Cambridge and further afield.
Impact This has just begun.
Start Year 2011
 
Description Lipidomics in a population eating the Mediterranean diet 
Organisation University of Bari
Country Italy 
Sector Academic/University 
PI Contribution FS and MVa have established a collaboration with the University of Bari, Italy, for a project that will translate our murine studies into human research of the link between de novo lipogensis and fatty liver disease. The MTA is now in place and the University of Bari received the final approval from the ethics committee for sending the serum samples. .
Collaborator Contribution Providing blood plasma samples from an on-going Italian cohort
Impact None to date - currently processing data.
Start Year 2015
 
Description MODO consortium 
Organisation MRC Harwell
Country United Kingdom 
Sector Academic/University 
PI Contribution LPS is part of the MRC Harwell Mouse Models of Diabetes and Obesity (MODO) consortium. This consortium aims to promote the use of mouse mdoels generated at MRC Harwell for use within the wider research community. JLG will attend a informatics training course on the 18th June to enable access to the mouse lines. It is hoped LPS will have access to specific models of type 2 diabetes through the pipeline.
Collaborator Contribution This has allowed us access to mouse models of great relevance to our work.
Impact We have publications in preparation.
Start Year 2012
 
Description Mass spectrometry imaging of lung cancer 
Organisation University of Cambridge
Country United Kingdom 
Sector Academic/University 
PI Contribution We have initiated a collaboration looking at the development of tumours in an inducible myc mouse model of cancer. Biochemistry will conduct the animal experiments and provide tissue, while we are performing the imaging.
Collaborator Contribution See abive
Impact None to date
Start Year 2014
 
Description MetaboLights EBI project 
Organisation EMBL European Bioinformatics Institute (EMBL - EBI)
Country United Kingdom 
Sector Academic/University 
PI Contribution We are working with the European Bioinformatics Institute to develop a central repository for metabolomic and lipidomic data for the international community.
Collaborator Contribution This has helped in our databasing and metadata descriptions supporting our collaborative work.
Impact This has produced a funded BBSRC grant to take forward the collaboration, a number of publications, and most recently a further application to the EU framework 7 program. In 2013 we have applied for further funding from the BBSRC and MRC to develop these databases.
Start Year 2011
 
Description Monitoring reactive oxygen species damage in human patients 
Organisation Medical Research Council (MRC)
Department MRC Centre for Obesity and Related Metabolic Diseases
Country United Kingdom 
Sector Public 
PI Contribution We have developed assays to monitor ROS damage in liver and kidney transplant patients and in patients with selenoprotein deficiencies.
Impact This collaboration has just begun.
Start Year 2011
 
Description Monitoring reactive oxygen species damage in human patients 
Organisation Medical Research Council (MRC)
Department MRC Mitochondrial Biology Unit
Country United Kingdom 
Sector Public 
PI Contribution We have developed assays to monitor ROS damage in liver and kidney transplant patients and in patients with selenoprotein deficiencies.
Impact This collaboration has just begun.
Start Year 2011
 
Description PMMR Collaboration with MRC MRL investigating energy expenditure on patients with defective thyroid hormone transporter 
Organisation University of Cambridge
Department Metabolic Research Laboratories
Country United Kingdom 
Sector Academic/University 
PI Contribution Edward Visser and Krish Chatterjee have approached PMMR (Michelle Venables) to collaborate on a project investigating energy expenditure in a group of patients with a defective thyroid hormone transporter (MCT8). The patients will be from a subset of a worldwide trial investigating the T3 analogue Triac. Energy expenditure will be measured before and after Triac treatment using the DLW technique. This project has been costed to cover all aspects of the study; isotope, kits, analysis and staff time. This comes to £12,991.78 for which he will be invoiced.
Collaborator Contribution TBC
Impact TBC
Start Year 2014
 
Description PPAR-alpha and inflammation in humans 
Organisation University of Pennsylvania
Department Institute for Translational Medicine and Therapeutics (ITMAT)
Country United States 
Sector Academic/University 
PI Contribution LPS is collaborating with the University of Pennsylvania to look at human blood plasma from a study looking at the role PPAR-alpha plays in preventing inflammation in adipose tissue. This is an NIH-sponsored clinical research study into the Fenofibrate and Omega-3 Fatty Acid Modulation of Endotoxemia inflammation of adipose tissue (a model for inflammation associated insulin resistance). This work nicely compliments the programme work for LPS and may lead to a proposal to the NIH if the experiments are successful. Given this, LPS would like to 'absorb' the costs into the Burning fat project of LPS in the expectation that this work adds value and may pave the way for further funds in the future. Jonathan Powell suggested that the LPS group also consider using their samples for hepcidin analysis (all 3 hepcidin variants) as an off-subject but "added value" idea. HNR would not be involved. The proposal was just for information.
Collaborator Contribution They are conducting the clinical trial which will provide the samples to be analyzed at HNR.
Impact None to date
Start Year 2012
 
Description Philips Industrial CASE studentship 
Organisation Koninklijke Philips Electronics N.V.
Department Philips
Country Global 
Sector Private 
PI Contribution We are collaborating with Philips to develop electronic sensors for metabolites which may be predictive for insulin resistance or the very early stages of type II diabetes. We are providing expertise in biomarkers of type II diabetes and in return they have provided us with an EPSRC Industrial CASE studentship.
Collaborator Contribution The student will visit Philips to produce sensors for key biomarkers.
Impact This has funded a CASE studentship which will start this academic year.
Start Year 2011
 
Description Polyunsaturated fatty acid biosynthesis and vasoconstriction 
Organisation University of Southampton
Department Faculty of Medicine
Country United Kingdom 
Sector Academic/University 
PI Contribution The Institute of Developmental Sciences, University of Southampton and Southampton General Hospital and LPS are applying to the BBSRC to fund a project entitled "Polyunsaturated fatty acid biosynthesis and vasoconstriction." We have previously collaborated and published papers in this area. The project involves the analysis of eicosanoids in blood and endothelial cells/blood vessel wall.
Collaborator Contribution Main applicant and conducting the biolgical studies.
Impact A previous manuscript.
Start Year 2010
 
Description Selcia Industrial CASE award 
Organisation Selcia
Country United Kingdom 
Sector Private 
PI Contribution We are conducting research into the use of yeast as a means to generate labelled lipid standards for the lipidomic community. We will provide expertise in lipid detection and yeast biology while Selcia will provide expertise in preparative LC and stable isotope work.
Collaborator Contribution We will make use of equipment at Selcia for preparative LC.
Impact Industrial CASE funding for the student.
Start Year 2011
 
Description Stratifying type 2 diabetes - Exeter university 
Organisation University of Exeter
Country United Kingdom 
Sector Academic/University 
PI Contribution LPS has been working with various diabetes research groups around the UK as part of the MRC/BIS initiative to promote greater links between clinical research and pharmaceutical drug development. The "Stratifying type 2 diabetes" consortium, led by Prof Hattersley of Exeter University have received favourable reviews and an indication of £2 million funding for two clinical trails to assess drug responses to anti-diabetics and how diet, exercise and weight loss interact with drug response. The funding is subject to satisfactory responses to reviewers comments and would be considered a pilot study towards larger studies planned across five years.
Collaborator Contribution Providing samples from clinical trials.
Impact Still waiting on outcomes
Start Year 2012
 
Description Study of lipotoxicity with MRC CORD 
Organisation Medical Research Council (MRC)
Department MRC Centre for Obesity and Related Metabolic Diseases
Country United Kingdom 
Sector Public 
PI Contribution We have been measuring changes in potentially lipotoxicity species in adipose and liver tissue from animal models of obesity and type II diabetes in conjunction with MRC CORD.
Collaborator Contribution Access to animal models and facilities
Impact This is a multi-discip;inary collaboration spanning analytical chemistry, biochemistry and animal physiology. This collaboration has produced a publication this year and a grant application to the BHF which will be submitted early next year.
Start Year 2011
 
Description Syrris collaboration to develop on-line extraction for MS analysis 
Organisation Syrris Ltd
Country United Kingdom 
Sector Private 
PI Contribution to develop technology for the combination of size exclusion chromatography with electrospray ionisation based mass spectrometry.
Collaborator Contribution Syrris provides the FLLEX technology and covers the patent application costs.
Impact patent application for which Syrris covers the costs
Start Year 2013
 
Description The PROMIS study 
Organisation University of Cambridge
Department Cambridge Institute of Public Health
Country United Kingdom 
Sector Academic/University 
PI Contribution We are developing a high throughput lipidomic screen for human blood plasma and this will be used to profile lipidomic changes in the PROMIS study - a epidemiology study of myocardial infarction in Pakistan.
Collaborator Contribution Our partners are making the samples available and also providing GWAS data to cross correlate with the lipidomic data/
Impact Further funding - we have used the results to obtain further funding and perform lipidomic analysis on the EPIC heart study.
Start Year 2011
 
Description The liver metabolome 
Organisation National Institutes of Health (NIH)
Country United States 
Sector Public 
PI Contribution This project is a collaboration with Professor Andrew Patterson, Penn State University, USA and Dr. Frank Gonzalez, NIH, USA. This project is to develop tools in metabolomics and lipidomics. Frank Gonzalez is a group leader at NIH and developed the PPAR- alpha null mouse which we have used previously to study metabolic regulation. The purpose of this proposal is to develop workflows and bioinformatics tools for studying the liver metabolome, and in particular the impact of fatty liver and insulin resistance in the liver. While primarily focussed on mice for the first two years, subsequent years will also have a human component using tissue samples from NIH.
Collaborator Contribution We are conducting animal studies together to better understand how the liver metabolome is influenced by drug and genetic intervention.
Impact This has produced a successful grant application to the NIH for funding.
Start Year 2011
 
Description The liver metabolome 
Organisation Penn State University
Department Department of Pharmacology
Country United States 
Sector Academic/University 
PI Contribution This project is a collaboration with Professor Andrew Patterson, Penn State University, USA and Dr. Frank Gonzalez, NIH, USA. This project is to develop tools in metabolomics and lipidomics. Frank Gonzalez is a group leader at NIH and developed the PPAR- alpha null mouse which we have used previously to study metabolic regulation. The purpose of this proposal is to develop workflows and bioinformatics tools for studying the liver metabolome, and in particular the impact of fatty liver and insulin resistance in the liver. While primarily focussed on mice for the first two years, subsequent years will also have a human component using tissue samples from NIH.
Collaborator Contribution We are conducting animal studies together to better understand how the liver metabolome is influenced by drug and genetic intervention.
Impact This has produced a successful grant application to the NIH for funding.
Start Year 2011
 
Description Thought Leader Award from Agilent 
Organisation Agilent Technologies
Country United States 
Sector Private 
PI Contribution Agilent have awarded a Thought Leader award to the head of group. This consists of a brand new ion mobility mass spectrometry plus substantial funds for research to develop this new piece of equipment. In turn we will develop new mass spectrometry tools based around ion mobility for lipid detection.
Collaborator Contribution see above.
Impact The collaboration starts in december 2014
Start Year 2014
 
Description Understanding the role lipotoxicity plays in hypertension and inflammation 
Organisation University of Sheffield
Department Department of Cardiovascular Science
Country United Kingdom 
Sector Academic/University 
PI Contribution This is a collaboration with Sheffield University to follow lipid mediators and ROS species in cell culture and animal models of vascular tone and right ventricular hypertrophy.
Collaborator Contribution They are providing assistance in cell culture techniques - particularly for co-cultures.
Impact This has led to a programme grant application to the British Heart Foundation.
Start Year 2011
 
Description Unilever funding for a PhD studentship 
Organisation Unilever
Country United Kingdom 
Sector Private 
PI Contribution Unilever have an interest in how diet influences diseases of dyslipidaemia and in particular the role epigenetics plays in this process. They have CASE funded a PhD student to work in this area.
Collaborator Contribution They will make their labs open to the student to perform experiments there.
Impact CASE funding for a PhD student.
Start Year 2011
 
Description Vascular tone and the effects of lipid mediators 
Organisation University of Southampton
Department Institute of Developmental Sciences
Country United Kingdom 
Sector Academic/University 
PI Contribution We are measuring lipid mediators for Southampton University to understand the role diet plays in vascular tone.
Collaborator Contribution We have one manuscript under review and one grant application submitted to the British Heart Foundation
Impact We have one publication under review and a grant application submitted to the BHF.
Start Year 2011
 
Description Xtreme everest 
Organisation University College London
Country United Kingdom 
Sector Academic/University 
PI Contribution Members of LPS will analyse samples from the Xtreme Everest 2 expedition. This expedition will take volunteers from Kathmandu to Everest basecamp to examine adaptation to low oxygen levels. This is the second expedition, with the first attracting much media interest and a Horizon program. LPS are particularly interested in the concept of induction of PPAR-alpha in human skeletal muscle at high altitude as this appears counterintuitive since this would stimulate fatty acid metabolism at high altitude which is more oxygen demanding in terms of the ATP produced compared with increased glycolysis and metabolism of glucose. Two members of LPS will also join the expedition for the first three weeks and spend three days at Everest basecamp.
Collaborator Contribution Organising the Xtreme Everest expedition
Impact None to date
Start Year 2012
 
Description 2nd Metabolomics Sardinian Scientific School 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact 2nd Metabolomics Sardinian Scientific School was aimed at post-grad students new to the field of metabolomics. We gave seminars and workshops in various tools and techniques in metabolomics.
Year(s) Of Engagement Activity 2016
 
Description BBC filming as part of a news report on fat metabolism 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Media (as a channel to the public)
Results and Impact With MRC Epidemiology we took part in a documentary by the BBC on fat intake. This has led to a number of other media contacts since then.
Year(s) Of Engagement Activity 2015
 
Description BD2K NIH workshop 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? Yes
Type Of Presentation Keynote/Invited Speaker
Geographic Reach International
Primary Audience Policymakers/politicians
Results and Impact JG attended a workshop for the NIH Big Data to Knowledge Initiative (BD2K). This is a workshop on community-based standards efforts related to scientific data. The NIH will sponsor his attendance at this workshop, which was held September 25 - 26 in Rockville, Maryland.

Advised the NIH on how best to roll out initiatives in this research area
Year(s) Of Engagement Activity 2013
 
Description Cambridge Science Week 2017 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact took part in Cambridge Science week and put on a display on personalised medicine and health using advanced biochemical techniques.
Year(s) Of Engagement Activity 2017
 
Description CoOrganiser of a Workshop on Metabolomics in Sardinia, Italy 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? Yes
Type Of Presentation keynote/invited speaker
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact We arranged a week long work shop on Sardinia and funded students to attend through a number of EU initiatives. The aim was to increase their experience and help them in their projects. We received very favourable feedback.

TBC
Year(s) Of Engagement Activity 2014
 
Description Contact with the BBC about a scientific program on diet and food 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact CW contacted by Milla Harrison from the BBC doing another Horizon programme - The Biology and Environmental factors behind Obesity. Wanted some advice on genes and genotyping.
Year(s) Of Engagement Activity 2015
 
Description Daily Mail piece 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Type Of Presentation Paper Presentation
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact JG was approached by the Daily Mail via MRC Head Office concerning Circadian rythmns in fat. This work was published in the newspaper.

We have since been contacted to help with an Horizon programme and a piece appeared in the Wallstreet journal too.
Year(s) Of Engagement Activity 2012
 
Description Danish-UK Metabolism meeting 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Taught postgrads how to use metabolomics and lipidomics to study aspects of type 2 diabetes and related disorders.
Year(s) Of Engagement Activity 2017
 
Description Debating matters - regional final judge 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? Yes
Geographic Reach Regional
Primary Audience Schools
Results and Impact We encouraged students to both debate and think about applying to the University of Cambridge

see above
Year(s) Of Engagement Activity 2014
 
Description Discussion with Trust me I'm a doctor 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Media (as a channel to the public)
Results and Impact I advised on a program on energy balance and dieting pills.

Still waiting for the program to air!
Year(s) Of Engagement Activity 2014
 
Description French Embassy Seed meeting 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact JLG, LR, AA and ZA were invited to the French Embassy to discuss collaborations with colleagues from INRA on metabolomics. This was following a successful application to the French government to fund the meeting by JLG and his counterparts in France.
Year(s) Of Engagement Activity 2015
 
Description Graduate School of Life Sciences, University of Cambridge 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? Yes
Geographic Reach Local
Primary Audience Postgraduate students
Results and Impact Introduction to statistics for graduates in the life sciences to help with their research

Has given the students added confidence to use these tools
Year(s) Of Engagement Activity 2014
 
Description Head Start science scheme for sixth form students 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Schools
Results and Impact We hosted approximately thirty sixth form students for a seminar on metabolism followed by an introduction to a typical undergraduate practical class. The practical class was augmented by a practical demonstration of metabolomics where the students analyzed their own urine by high resolution 1H NMR spectroscopy and computer assisted pattern recognition to determine who was male and who was female!

We have already had a request to continue this workshop next year. A number of the students have since applied to Oxbridge despite being from schools that typically do not send students to the top universities.
Year(s) Of Engagement Activity 2011
 
Description Hosted a visit from Singaporean students 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Type Of Presentation Keynote/Invited Speaker
Geographic Reach International
Primary Audience Schools
Results and Impact We hosted a visit from 6th form Singaporean students interested in applying to Cambridge as undergraduates in science related areas.

They gave me a box of chocolates!
Year(s) Of Engagement Activity 2013
 
Description How the fat flies - Science Week 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact The University of Cambridge Science Festival took place on Saturday 14th and Sunday 15th March. My group had a stand at the Corn Exchange. The stand was split into two, with one half focusing on nutrition and the other on mass spectrometry.
Year(s) Of Engagement Activity 2015
 
Description Introduction to Systems Biology Course delivered to school children at St. John's College (led by James Smith) 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact TBC
Year(s) Of Engagement Activity 2014
 
Description Junior school science talk 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation Workshop Facilitator
Geographic Reach Local
Primary Audience Schools
Results and Impact We have given science talks on temperature and food to approximately fifty eight year olds.

We are taking part in a larger science fair to be organised this coming year.
Year(s) Of Engagement Activity 2011,2012
 
Description LPS at Science week 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? Yes
Type Of Presentation Workshop Facilitator
Geographic Reach Regional
Primary Audience Schools
Results and Impact The Cambridge Science festival attracts 1000s of members of the public to the centre of cambridge to see various displays of science in action. We took part in the HNR's contribution, explaining mass spectrometry and its role in obesity and diabetes research.

None to date.
Year(s) Of Engagement Activity 2012
 
Description Media Coverage - Everest challenge 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Type Of Presentation Keynote/Invited Speaker
Geographic Reach Regional
Primary Audience Media (as a channel to the public)
Results and Impact 1 regional radio interivew and article in regional newspaper for taking part in Extreme Everest challenge.

Building up links with media, disseminating research to media and public, and raising profile of unit's work.
Year(s) Of Engagement Activity 2013
 
Description MetaboFlow and Cambridge Metabolomics 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Industry/Business
Results and Impact Held a Metabolomics Interest group for the East Anglia/London region to increase interest in this research area.
Year(s) Of Engagement Activity 2017
 
Description Metabolic Complications in Obesity 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact A conference/workshop to promote new methods for understanding the causes and consequences of obesity.
Year(s) Of Engagement Activity 2017
 
Description Metabolomics Workshop at the 20th International Symposium on Microsomes and drug oxidations, stuttgart, germany 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? Yes
Geographic Reach International
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact academics said they would incorporate metabolomics into their future experiments

See above
Year(s) Of Engagement Activity 2014
 
Description Metabolomics workshop for industry 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Type Of Presentation Workshop Facilitator
Geographic Reach International
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact Members of LPS co-organised a European Bioinformatics Instuitute Industrail Collaboration Workshop on Metabolomics and Lipidomics. Albert Koulman and Michael Eiden also attended this meeting.

This raised the profile of our research with industry.
Year(s) Of Engagement Activity 2012
 
Description Pint of science 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Talked about the research behind the debate about whether it is increased carbohydrate or saturated fat intake that is driving the obesity and type 2 diabetes epidemics.
Year(s) Of Engagement Activity 2017
 
Description Poster presentation at Experimental Biology 2015 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact One of the PhD students attended Experimental Biology and presented a poster entitled "Effects of green tea and coffee polyphenols on cardiometabolic function in women with Polycystic Ovary Syndrome." Experimental Biology Boston, USA, 28 March-1 April 2015.
Year(s) Of Engagement Activity 2015
 
Description Presentation at the European Association for the study of Diabetes Conference in Vienna, September 2014 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? Yes
Geographic Reach International
Primary Audience Health professionals
Results and Impact TBC
Year(s) Of Engagement Activity 2014
 
Description Press release for circadian rhythms 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Type Of Presentation Paper Presentation
Geographic Reach National
Primary Audience Media (as a channel to the public)
Results and Impact We produced a press release on work published in Nature Medicine on circadian rhythms in adipose tissue.

This led to contact with a journalist producing an article on obesity and its link with sleep.
Year(s) Of Engagement Activity 2012
 
Description Sardinian summer school: Metabolomics and more. 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Sardinian summer school to spread the use of tools in metabolomics and lipidomics.
Year(s) Of Engagement Activity 2017
 
Description Science Week cambridge 2016 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact : volunteering on 13th March 2016 Cambridge science festival HNR stand: Blood, Fat & the Future
Year(s) Of Engagement Activity 2016
 
Description Science career advice to prospective undergraduates 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Schools
Results and Impact This was a talk as part of an open day In cambridge to encourage students to study Natural sciences at Cambridge.

I received a number of requests for further information from students.
Year(s) Of Engagement Activity 2011,2012,2014
 
Description Small Molecule Inter-organ Crosstalk and the Browning of White Adipose Tissue talk, School of Biomedical Sciences, University of Leeds (Lee Roberts) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact TBC
Year(s) Of Engagement Activity 2014
 
Description Sugar, Fat and the Daily Mail Headlines 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact JLG spoke at the 'Skeptics in the Pub' event on 26th May. The video is on YouTube should anyone want to view it!
Year(s) Of Engagement Activity 2015
 
Description Talk on metabolomics at Bedford School 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Gave a talk on metabolomics and systems biology to the biology club at Bedford school.
Year(s) Of Engagement Activity 2015
 
Description Warburg and the War on cancer: Metabolism and treating cancer 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Gave a talk at the Experimental Biology meeting detailing how metabolomics can be used to treat cancer in the future.
Year(s) Of Engagement Activity 2015
 
Description West African Centre for cell Biology of Infectious Pathogens College of Basic and Applied Sciences, Ghana Research Conference 2017 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Took part in a 3 day conference/workshop to discuss advances in Biology that could be applied within Western Africa to treat human disease.
Year(s) Of Engagement Activity 2017
 
Description Work experience for two school boys 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Schools
Results and Impact Work experience for two school boys: two school boys spent a week in my lab following members around to get some experience of what its like being a scientist.
Year(s) Of Engagement Activity 2016
 
Description Workshop at Metabolomics Society 2013 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? Yes
Type Of Presentation Workshop Facilitator
Geographic Reach International
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact ~100 active researchers attended the workshop.

None to date
Year(s) Of Engagement Activity 2013
 
Description Workshop at the Wellcome Trust roganised by the WT/Diabetes Juvenille Diabetes Foundation on autoimmune diseases 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact Talk sparked questions and discussion after

I have initiated one collaboration and had other talk invitations on the back of my presentation.
Year(s) Of Engagement Activity 2014
 
Description Workshop at the university of cambridge to train students in physiology 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Type Of Presentation Workshop Facilitator
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Members of LPS are contributing to a four day workshop sponsored by the European Atheroscelrosis Society on Phenotyping mouse models of the Metabolic Syndrome.

None to date.
Year(s) Of Engagement Activity 2012
 
Description Workshop on New MS-tools, Wageningen, Natherlands 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact Talk sparked questions and discussions afterwards

We have continued discussing new technologies and how we might incorporate them into our workflows
Year(s) Of Engagement Activity 2014