Entero-endocrinology including the effects of bariatric surgery
Lead Research Organisation:
University of Cambridge
Department Name: UNLISTED
Abstract
The gut releases signals that coordinate how it handles food, how nutrients are distributed in the body and how much food is consumed. Modifying these signals presents an opportunity to treat obesity and high blood sugar and it is thought that changes in these signals are important in the positive effects of bariatric surgery. We use mice that have been genetically altered to either label the cells that produce these signals or the cells that receive the signals, enabling us to identify and characterise changes when these mice undergo bariatric surgery. We hope that understanding these changes will, in the long term, enable us to modify gut-derived signals to treat metabolic diseases in humans without the need of surgical intervention.
Technical Summary
The enteroendocrine system is a key player in the control of postprandial nutrient homeostasis, modulating the transit and digestion of nutrients within the intestine, the disposal of nutrients in peripheral organs and affecting future feeding behaviour/appetite. Some peptide hormones, notably glucagon-like peptide-1, are already exploited to treat diabetes and obesity, whilst changes in enteroendcrine responses are thought to contribute to the beneficial metabolic outcomes of bariatric surgery. This programme uses genetically modified mouse models to address the mechanisms underlying enteroendocrine changes in response to bariatric surgery with the aim to modulate the enteroendocrine system for the treatment of metabolic disease. We compare in vivo results in mice after vertical sleeve gastrectomy with observations in human volunteers, which have undergone gastrectomy with Roux-en-Y type reconstruction of the upper small intestine and in vitro studies with primary mixed epithelial cultures and intestinal organoids of both human and murine origin. Transcriptomic, mass-spec-based peptidomic, optical single cell second messenger dynamic and electrophysiological investigations are combined with pharmacological and/or genetic manipulations to identify and characterise signalling mechanisms underlying enteroendocrine hormone release and action.
Organisations
- University of Cambridge (Lead Research Organisation)
- California Institute of Technology (Collaboration)
- University of Copenhagen (Collaboration)
- Royal Netherlands Academy of Arts and Sciences (Collaboration)
- University College London (Collaboration)
- Université Catholique de Louvain (Collaboration)
- UNIVERSITY OF CAMBRIDGE (Collaboration)
- French National Institute of Agricultural Research (Collaboration)
- Autonomous University of Madrid (Collaboration)
- AstraZeneca (United Kingdom) (Collaboration)
- UNIVERSITY OF ABERDEEN (Collaboration)
- University of Ulster (Collaboration)
- Mayo Clinic (Collaboration)
- Eli Lilly & Company Ltd (Collaboration)
- Florida State University (Collaboration)
- LGC Ltd (Collaboration)
- Mercodia AB (Collaboration)
- Addenbrooke's Hospital (Collaboration)
- University of Toronto (Collaboration)
People |
ORCID iD |
Publications
Adriaenssens AE
(2018)
Distribution and Stimulus Secretion Coupling of Enteroendocrine Cells along the Intestinal Tract.
in Comprehensive Physiology
Adriaenssens AE
(2019)
Glucose-Dependent Insulinotropic Polypeptide Receptor-Expressing Cells in the Hypothalamus Regulate Food Intake.
in Cell metabolism
Adriaenssens AE
(2020)
The glucose-dependent insulinotropic polypeptide signaling axis in the central nervous system.
in Peptides
Arora T
(2018)
Microbial regulation of the L cell transcriptome
in Scientific Reports
Ast J
(2020)
Super-resolution microscopy compatible fluorescent probes reveal endogenous glucagon-like peptide-1 receptor distribution and dynamics
in Nature Communications
Augustine V
(2018)
Hierarchical neural architecture underlying thirst regulation.
in Nature
Beumer J
(2018)
Enteroendocrine cells switch hormone expression along the crypt-to-villus BMP signalling gradient.
in Nature cell biology
Biancolin AD
(2020)
The core clock gene, Bmal1, and its downstream target, the SNARE regulatory protein secretagogin, are necessary for circadian secretion of glucagon-like peptide-1.
in Molecular metabolism
Biglari N
(2021)
Functionally distinct POMC-expressing neuron subpopulations in hypothalamus revealed by intersectional targeting
in Nature Neuroscience
Billing LJ
(2018)
Co-storage and release of insulin-like peptide-5, glucagon-like peptide-1 and peptideYY from murine and human colonic enteroendocrine cells.
in Molecular metabolism
Brierley DI
(2021)
Central and peripheral GLP-1 systems independently suppress eating.
in Nature metabolism
Calderon RM
(2022)
Intestinal Enteroendocrine Cell Signaling: Retinol-binding Protein 2 and Retinoid Actions.
in Endocrinology
Campbell JE
(2022)
GIPR Is Predominantly Localized to Nonadipocyte Cell Types Within White Adipose Tissue.
in Diabetes
Campbell JR
(2020)
Essential Role of Syntaxin-Binding Protein-1 in the Regulation of Glucagon-Like Peptide-1 Secretion.
in Endocrinology
Chae H
(2020)
SGLT2 is not expressed in pancreatic a- and ß-cells, and its inhibition does not directly affect glucagon and insulin secretion in rodents and humans.
in Molecular metabolism
Church D
(2018)
Assessment and Management of Anti-Insulin Autoantibodies in Varying Presentations of Insulin Autoimmune Syndrome
in The Journal of Clinical Endocrinology & Metabolism
Cimino I
(2021)
Murine neuronatin deficiency is associated with a hypervariable food intake and bimodal obesity.
in Scientific reports
Coll AP
(2020)
GDF15 mediates the effects of metformin on body weight and energy balance.
in Nature
Coll AP
(2020)
Publisher Correction: GDF15 mediates the effects of metformin on body weight and energy balance.
in Nature
Cyranka M
(2019)
Abcc5 Knockout Mice Have Lower Fat Mass and Increased Levels of Circulating GLP-1.
in Obesity (Silver Spring, Md.)
Denwood G
(2019)
Glucose stimulates somatostatin secretion in pancreatic d-cells by cAMP-dependent intracellular Ca2+ release.
in The Journal of general physiology
Dye FS
(2019)
Characterisation of proguanylin expressing cells in the intestine - evidence for constitutive luminal secretion.
in Scientific reports
Fang Z
(2020)
Ligand-Specific Factors Influencing GLP-1 Receptor Post-Endocytic Trafficking and Degradation in Pancreatic Beta Cells.
in International journal of molecular sciences
Foreman RE
(2022)
LC-MS/MS based detection of circulating proinsulin derived peptides in patients with altered pancreatic beta cell function.
in Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
Foreman RE
(2023)
Optimized LC-MS/MS Method for the Detection of ppCCK(21-44): A Surrogate to Monitor Human Cholecystokinin Secretion.
in Journal of proteome research
Galvin SG
(2021)
The Human and Mouse Islet Peptidome: Effects of Obesity and Type 2 Diabetes, and Assessment of Intraislet Production of Glucagon-like Peptide-1.
in Journal of proteome research
George AL
(2023)
Rapid and Quantitative Enrichment of Peptides from Plasma for Mass Spectrometric Analysis.
in Methods in molecular biology (Clifton, N.J.)
Goldspink DA
(2020)
Labeling and Characterization of Human GLP-1-Secreting L-cells in Primary Ileal Organoid Culture.
in Cell reports
Goldspink DA
(2018)
Mechanistic insights into the detection of free fatty and bile acids by ileal glucagon-like peptide-1 secreting cells.
in Molecular metabolism
Goldspink DA
(2018)
Models and Tools for Studying Enteroendocrine Cells.
in Endocrinology
Gribble F
(2018)
Physiology of the Gastrointestinal Tract
Gribble F
(2022)
Nutrient sensing in the gut and the regulation of appetite
in Current Opinion in Endocrine and Metabolic Research
Gribble FM
(2019)
Function and mechanisms of enteroendocrine cells and gut hormones in metabolism.
in Nature reviews. Endocrinology
Gribble FM
(2021)
Metabolic Messengers: glucagon-like peptide 1.
in Nature metabolism
Gribble FM
(2021)
Obesity therapeutics: The end of the beginning.
in Cell metabolism
Guccio N
(2022)
Glucose-Dependent Insulinotropic Polypeptide-A Postprandial Hormone with Unharnessed Metabolic Potential.
in Annual review of nutrition
Guida C
(2019)
PYY plays a key role in the resolution of diabetes following bariatric surgery in humans.
in EBioMedicine
Hayashi M
(2023)
Enteroendocrine cell lineages that differentially control feeding and gut motility.
in eLife
Heeley N
(2018)
Rapid sensing of l-leucine by human and murine hypothalamic neurons: Neurochemical and mechanistic insights.
in Molecular metabolism
Holt MK
(2019)
Synaptic Inputs to the Mouse Dorsal Vagal Complex and Its Resident Preproglucagon Neurons.
in The Journal of neuroscience : the official journal of the Society for Neuroscience
Holt MK
(2020)
PPG neurons in the nucleus of the solitary tract modulate heart rate but do not mediate GLP-1 receptor agonist-induced tachycardia in mice.
in Molecular metabolism
Jepsen SL
(2019)
Paracrine crosstalk between intestinal L- and D-cells controls secretion of glucagon-like peptide-1 in mice.
in American journal of physiology. Endocrinology and metabolism
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| Project Reference | Relationship | Related To | Start | End | Award Value |
|---|---|---|---|---|---|
| MC_UU_00014/1 | 01/04/2018 | 31/03/2024 | £2,995,583 | ||
| MC_UU_00014/2 | Transfer | MC_UU_00014/1 | 01/04/2018 | 31/03/2024 | £2,871,979 |
| MC_UU_00014/3 | Transfer | MC_UU_00014/2 | 01/04/2018 | 31/03/2024 | £1,667,444 |
| MC_UU_00014/4 | Transfer | MC_UU_00014/3 | 01/04/2018 | 31/03/2024 | £1,743,788 |
| MC_UU_00014/5 | Transfer | MC_UU_00014/4 | 01/04/2018 | 31/03/2024 | £3,355,490 |
| MC_UU_00014/6 | Transfer | MC_UU_00014/5 | 01/04/2018 | 31/03/2024 | £1,325,715 |
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| Organisation | European Association for the Study of Diabetes (EASD) |
| Department | European Foundation for the Study of Diabetes (EFSD) |
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| Description | ESE St Odile meeting grant |
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| Organisation | European Society of Endocrinology |
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| Description | Jo Lewis, BSN symposium support for Physiology 2019, Aberdeen, UK |
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| Description | Jo Lewis, The Physiological Society symposium support for Experimental Biology, Orlando, |
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| Description | MRC Equip - World Class Labs award 2022/23 |
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| Funding ID | MC_PC_MR/X012417/1 |
| Organisation | Medical Research Council (MRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 03/2023 |
| Description | MRC Equip - World Class Labs award 2022/23 - host contribution |
| Amount | £125,963 (GBP) |
| Organisation | University of Cambridge |
| Sector | Academic/University |
| Country | United Kingdom |
| Start | 03/2023 |
| Description | Targeting the gut in metabolic disease |
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| Funding ID | 220271/Z/20/Z |
| Organisation | Wellcome Trust |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 08/2020 |
| End | 07/2025 |
| Description | Wellcome PhD studentship to Elisabeth O'Flaherty |
| Amount | £180,000 (GBP) |
| Organisation | Wellcome Trust |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 10/2019 |
| End | 09/2022 |
| Description | Wellcome PhD studentship to Emily Miedzybrodzka |
| Amount | £180,824 (GBP) |
| Organisation | Wellcome Trust |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 10/2018 |
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| Title | Gipr-Cre transgenic mouse |
| Description | GIPR-Cre mouse enabling identification and manipulation of cells expressing Gipr |
| Type Of Material | Model of mechanisms or symptoms - mammalian in vivo |
| Year Produced | 2016 |
| Provided To Others? | Yes |
| Impact | Collaborations and publications. Research collaboration with Eli Lilly, investigating MOA of GLP1/GIP dual agonists (currently in phase 3 clinical trials) |
| Title | Glu-CreERT2 |
| Description | Tamoxifen-inducible cre expression driven by the proglucagon promoter |
| Type Of Material | Model of mechanisms or symptoms - mammalian in vivo |
| Year Produced | 2017 |
| Provided To Others? | Yes |
| Impact | Shared with academic collaborators and licensed to industry |
| Title | Guanylin-Venus transgenic mouse |
| Description | Guanylin-venus mouse, enabling identification and characterisation of cells expressing guanylin |
| Type Of Material | Model of mechanisms or symptoms - mammalian in vivo |
| Year Produced | 2019 |
| Provided To Others? | Yes |
| Impact | Publication |
| Title | Insl5-rtTA mouse |
| Description | transgenic mouse Insl5-rtTA allowing identification and manipulation of INSL5-expressing cells |
| Type Of Material | Model of mechanisms or symptoms - mammalian in vivo |
| Year Produced | 2018 |
| Provided To Others? | Yes |
| Impact | publications |
| Title | Rxfp4-Cre transgenic mouse |
| Description | Rxfp4-Cre transgenic mouse |
| Type Of Material | Model of mechanisms or symptoms - mammalian in vivo |
| Year Produced | 2015 |
| Provided To Others? | No |
| Impact | BBSRC-iCASE studentship joint with AstraZeneca to investigate central signalling involving Rxfp4 |
| Title | Alteration of the plasma peptidome of gastrectomised patients during an OGTT |
| Description | Performing gastric bypass surgery on patients severely changes their gut hormone response to glucose. The plasma concentrations of Insulin and a number of associated incretin peptides are significantly raised shortly after ingestion of glucose. Typically the plasma concentrations of these peptides are monitored using immunoassays. A simple and high throughput plasma peptidomics approach was used to simulatneously monitor a large number of these peptides. Comparison of gastresctomised and control individuals showed significant increases of insulin, glucagon gene products, PYY and neurotensin. |
| Type Of Material | Database/Collection of data |
| Year Produced | 2020 |
| Provided To Others? | Yes |
| Impact | open dataset |
| URL | https://www.ebi.ac.uk/pride/archive/projects/PXD016690 |
| Title | Characterization of Guca2a expressing cells in the mouse intestine |
| Description | RNA seq of Guca2a expressing cells |
| Type Of Material | Database/Collection of data |
| Year Produced | 2019 |
| Provided To Others? | Yes |
| Impact | openly accessible data |
| URL | https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE128117 |
| Title | Co-storage and release of insulin-like peptide-5, glucagon-like peptide-1 and peptideYY from murine and human colonic enteroendocrine cells |
| Description | Analysis of vesicular colocalisation by 3D-SIM |
| Type Of Material | Data analysis technique |
| Year Produced | 2018 |
| Provided To Others? | Yes |
| Impact | open method |
| Title | Expected values for gastrointestinal and pancreatic hormone concentrations in healthy volunteers in the fasting and postprandial state |
| Description | Background: Gastrointestinal hormones regulate intestinal transit, control digestion, influence appetite and promote satiety. Altered production or action of gut hormones, including glucagon-like peptide-1 (GLP-1), glucose-dependent insulinotropic polypeptide (GIP) and peptide YY (PYY), may contribute to the biological basis of obesity and altered glucose homeostasis. However, challenges in analytical methodology and lack of clarity on expected values for healthy individuals has limited progress in this field. The aim of this study was to describe expected concentrations of gastrointestinal and pancreatic hormones in healthy volunteers following a standardised meal test (SMT) or 75g oral glucose tolerance test (OGTT). Methods: 28 healthy volunteers (12 men, 16 women; mean age 31.3 years; mean body mass index 24.9 kg/m2) were recruited to attend a hospital clinic on two occasions. Volunteers had blood sampling in the fasting state and were given, in randomised order, an oral glucose tolerance test (OGTT) and standardised mixed liquid meal test with venepuncture at timed intervals for 4 hours after ingestion. Analytical methods for gut and pancreatic hormones were assessed and optimised. Concentrations of gut and pancreatic hormones were measured and used to compile ranges of expected values. Results: Ranges of expected values were created for glucose, insulin, glucagon, GLP-1, GIP, PYY and free fatty acids in response to a standardised mixed liquid meal or OGTT. Intact Proinsulin and C-peptide levels were also measured following the OGTT. Conclusions: These ranges of expected values can now be used to compare gut hormone concentrations between healthy individuals and patient groups. |
| Type Of Material | Database/Collection of data |
| Year Produced | 2020 |
| Provided To Others? | Yes |
| Impact | Openly accessible dataset |
| URL | https://www.repository.cam.ac.uk/handle/1810/312528 |
| Title | Glucose-dependent Insulinotropic polypeptide receptor-expressing cells in the hypothalamus regulate food intake |
| Description | Ambiguity regarding the role of glucose-dependent insulinotropic polypeptide (GIP) in obesity arises from conflicting reports asserting that both GIP receptor (GIPR) agonism and antagonism are effective strategies for inhibiting weight gain. To enable identification and manipulation of Gipr-expressing (Gipr) cells we created GIPR-Cre knock-in mice. As GIPR-agonists have recently been reported to suppress food intake we aimed to identify central mediators of this effect. Gipr cells were identified in the arcuate, dorsomedial, and paraventricular nuclei of the hypothalamus, as confirmed by RNAscope in mouse and human. Single cell RNAseq identified clusters of hypothalamic Gipr cells exhibiting transcriptomic signatures for mural, glial and neuronal cells, the latter expressing somatostatin, but little proopiomelanocortin or agouti-related peptide. Activation of Gq-DREADDs in hypothalamic Gipr cells suppressed food intake in vivo, which was not obviously additive with concomitant GLP1R activation. These data identify hypothalamic GIPR as a target for the regulation of energy balance. |
| Type Of Material | Database/Collection of data |
| Year Produced | 2019 |
| Provided To Others? | Yes |
| Impact | openly accessible dataset |
| URL | https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE134726 |
| Title | Human enteroendocrine cell transcriptomic profiling |
| Description | We purified two populations of human jejunal enteroendocrine cells (GLP1+ and GLP1-) by FACS and identified transcripts enriched in endocrine cell lineages. |
| Type Of Material | Database/Collection of data |
| Year Produced | 2018 |
| Provided To Others? | Yes |
| Impact | open accessible dataset |
| URL | https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE114853 |
| Title | Human intestinal organoid cell transcriptomic profiling |
| Description | We purified two populations of human ileal enteroendocrine cells (Venus+ and Venus-) from mature differentiated hGLU-Venus organoids by FACS and identified transcripts enriched in endocrine cell lineages. |
| Type Of Material | Database/Collection of data |
| Year Produced | 2020 |
| Provided To Others? | Yes |
| Impact | openly accessible dataset |
| URL | https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE148224 |
| Title | Mouse duodenal enteroendocrine transcriptome |
| Description | Using genetically modifed mice to express fluorescent reporter in proglucagon expressing cells (GluVenus) or in all enteroendocrine cells (NeuroD1), we purifed positive and negative cells by FACS from duodenal epithelial cells and identified transcripts enriched in positive populations |
| Type Of Material | Database/Collection of data |
| Year Produced | 2018 |
| Provided To Others? | Yes |
| Impact | open accessible dateset |
| URL | https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE114913 |
| Title | Peptidomic analysis of pancreatic neurodendocrine tumours (Glucagonoma) |
| Description | A peptidomic analysis of plasma from patients with well characterised pancreatic neuroendocrine tumours (PNET) was performed. A nano LC-MS analysis identified a number of peptides from the glucagon gene, which were idenfiried in a previous case study using multiple immunoassays. Peptides to other proteins involved in peptide secretion were also identified. Plasma from a second glucagonoma patient was analysed using a high flow approach, and this identified similar peptides to the nano analysis. In order to demonstrate the potential clinical application of LC-MS to characterising neuroendocrine tumours, a large cohort of plasma samples were analysed to demonstrate the ability of this approach to identify glucagonoma patients, and differentiate from a single insulinoma patient |
| Type Of Material | Database/Collection of data |
| Year Produced | 2018 |
| Provided To Others? | Yes |
| Impact | open dataset |
| URL | https://www.ebi.ac.uk/pride/archive/projects/PXD008465 |
| Title | Peptidomics of sorted enteroendocrine cells from human organoids |
| Description | A human organoid culture system was set up to grow enteroendocrine cells with a venus labeled on the glucagon gene promoter sequence. This enabled the sorting of glucagon gene positive cells from negative cells, thereby enabling the enrichment of glucagon producing cells for study. Both Venus positive and venus negative cell populations were collected and their peptidome was assessed using nano LC-MS/MS |
| Type Of Material | Database/Collection of data |
| Year Produced | 2020 |
| Provided To Others? | Yes |
| Impact | open data |
| URL | https://www.ebi.ac.uk/pride/archive/projects/PXD017825 |
| Title | Peptidomics profiling of biopsies from the human jejunum before and after gastrectomy |
| Description | Profiling of the human jejunum peptidome before and after total gastrectomy. The study compares the peptidome of jejunum using intact mass spectrometry and database searching. These peptides included most gut hormones including proglucagon derived peptides, PYY, GIP, Motilin somatostatin and neurotensin amongst others. |
| Type Of Material | Database/Collection of data |
| Year Produced | 2019 |
| Provided To Others? | Yes |
| Impact | open dataset |
| URL | https://www.ebi.ac.uk/pride/archive/projects/PXD011498 |
| Title | Peptidomics profiling of human intestine |
| Description | The aim of the project is to generate a peptidomics map of gut hormone peptides along the gastrointestinal tract, starting with the stomach and including the duodenum, jejunum, ileum, ascending colon, sigmoid colon and rectum. The tissues would be collected after surgery and the peptide fraction extracted and anlysed by nano LC-MS to identify what peptide hormones are present. These data will then be used to compare against the human transcriptome, and also for comparison against equivent peptides from murine intestinal extracts. |
| Type Of Material | Database/Collection of data |
| Year Produced | 2019 |
| Provided To Others? | Yes |
| Impact | open dataset |
| URL | https://www.ebi.ac.uk/pride/archive/projects/PXD009788 |
| Title | Peptidomics profiling of murine intestine |
| Description | The aim of the project is to generate a peptidomics map of gut hormone peptides along the gastrointestinal tract, starting with the stomach and including the duodenum, jejunum, ileum, colon and rectum. The tissues would be collected after surgery and the peptide fraction extracted and anlysed by nano LC-MS to identify what peptide hormones are present. These data will then be used to compare against the murine transcriptome, and also for comparison against equivent peptides from human intestinal extracts. |
| Type Of Material | Database/Collection of data |
| Year Produced | 2019 |
| Provided To Others? | Yes |
| Impact | open dataset |
| URL | https://www.ebi.ac.uk/pride/archive/projects/PXD009796 |
| Title | Peptidomics profiling of the murine gut after vertical sleeve gastrectomy |
| Description | Profiling of the murine peptidome along the gastrointestinal tract and pancreas in vertical sleeve gastrectomy and sham operated mice. The study compares the peptidome of various portions of the GI tract using intact mass spectrometry and database searching. These peptides included most gut hormones including proglucagon derived peptides, PYY, GIP, somatostatin, neurotensin amongst others. |
| Type Of Material | Database/Collection of data |
| Year Produced | 2019 |
| Provided To Others? | Yes |
| Impact | open dataset |
| URL | https://www.ebi.ac.uk/pride/archive/projects/PXD011455 |
| Title | Single cell transcriptomic profiling of large intestinal enteroendocrine cells in mice |
| Description | We report the different enteroendocrine populations in the large intestine in mice based on their transcriptomics profile. Hormone, receptor and transcription factors are analysed for differential expression between the different cell clusters to give a comprehensive view of the different characterisitcs of the distal entereondocrine cells |
| Type Of Material | Database/Collection of data |
| Year Produced | 2019 |
| Provided To Others? | Yes |
| Impact | openly accessible dataset |
| URL | https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE137572 |
| Title | The effect on the transcriptome by inhibition of mitochondrial function by metformin in intestinal cells |
| Description | We purified mouse duodenal cells from organoids derived from two different transgenic mouse lines (GIP-Cre tdRFP, SST-Cre tdRFP), and identified transcripts enriched in metformin-treated cells. |
| Type Of Material | Database/Collection of data |
| Year Produced | 2021 |
| Provided To Others? | Yes |
| Impact | openly accessible dataset |
| URL | https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE165122 |
| Title | Transcriptomics analysis of enteroendocrine cells after vertical sleeve gastrectomy |
| Description | We report here the transcriptome of sorted enteroendocrine cells from duodenum, ileum and colon from mice after vertical sleeve gastrectomy or sham operation, weight matched or not. We analyze the effect of region of origin and surgery and gene expression and found that only region of origin had an impact on EEC transcriptome |
| Type Of Material | Database/Collection of data |
| Year Produced | 2018 |
| Provided To Others? | Yes |
| Impact | open dataset |
| URL | https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE121489 |
| Title | Transcriptomics analysis of human jejunal enteroendocrine cells after vertical sleeve gastrectomy |
| Description | We report here the transcriptome of a population enriched in enteroendocrine cells from human jejunum from patients before or after gastrectomy. We analyze the effect of surgery on gene expression and found very little effect on EEC transcriptome |
| Type Of Material | Database/Collection of data |
| Year Produced | 2018 |
| Provided To Others? | Yes |
| Impact | open dataset |
| URL | https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE121486 |
| Description | Acosta |
| Organisation | Mayo Clinic |
| Country | United States |
| Sector | Charity/Non Profit |
| PI Contribution | Training provided to Acosta's group |
| Collaborator Contribution | Analysis of patient samples |
| Impact | None |
| Start Year | 2018 |
| Description | Alice Adriaenssens |
| Organisation | University College London |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Alice was previously a member of our research team but now has a lecturer position at UCL. Our collaboration continues, and she continues work with our mouse models and using some of our facilities. |
| Collaborator Contribution | Funded by Alice's new grants. |
| Impact | Publications |
| Start Year | 2022 |
| Description | Ana Garcia nanoparticles |
| Organisation | Catholic University of Louvain |
| Country | Belgium |
| Sector | Academic/University |
| PI Contribution | We are hosting a post doc in our group |
| Collaborator Contribution | Our partners are funding the project and provided the initial study design |
| Impact | Extension of the partnership from 1 year to 2 years |
| Start Year | 2020 |
| Description | AstraZeneca |
| Organisation | AstraZeneca |
| Department | Research and Development AstraZeneca |
| Country | United Kingdom |
| Sector | Private |
| PI Contribution | A series of iCASE studentships, funded mostly by UKRI. We provide student training and supervision, as well as project design etc. |
| Collaborator Contribution | AZ supervisors provide funding, training, access to facilities and tool reagents. |
| Impact | Multiple publications, as well as a continuous dialogue that has enhanced research by both parties. |
| Start Year | 2014 |
| Description | Brubaker lab |
| Organisation | University of Toronto |
| Country | Canada |
| Sector | Academic/University |
| PI Contribution | Mouse models and discussion |
| Collaborator Contribution | Experimentation and project design |
| Impact | Publication |
| Start Year | 2015 |
| Description | Central GLP1 signalling |
| Organisation | University College London |
| Department | Biosciences |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Longstanding collaboration with Stefan Trapp's laboratory. We currently (Mar 21) have a joint application for further funding from MRC pending, which would financially contribute to the Reimann/Gribble laboratories. We frequently exchange ideas, have provided critical mouse models for this research and transcriptomic capabilities. |
| Collaborator Contribution | see above |
| Impact | Prof. Trapp's and the Reimann/Gribble laboratory have so far co-published 18 research papers on the topic (as on 10Mar21) |
| Description | G/R Bertie Goettgens, Single Cell RNAseq |
| Organisation | University of Cambridge |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Experiments |
| Collaborator Contribution | Experiments |
| Impact | none yet |
| Start Year | 2015 |
| Description | GIP receptor localisation and function |
| Organisation | University of Toronto |
| Country | Canada |
| Sector | Academic/University |
| PI Contribution | We generated a gipr knockin mouse, which has been shipped to Toronto |
| Collaborator Contribution | Analysis of the genetically modified mouse in Toronto |
| Impact | None to date |
| Start Year | 2016 |
| Description | Gastrectomy clinicians |
| Organisation | Addenbrooke's Hospital |
| Country | United Kingdom |
| Sector | Hospitals |
| PI Contribution | Physiological studies on humans after gastrectomy plus in vitro analysis of tissue samples |
| Collaborator Contribution | Identification of suitable patients |
| Impact | Publications |
| Start Year | 2015 |
| Description | Generation of organoids from transgenic models |
| Organisation | Royal Netherlands Academy of Arts and Sciences |
| Department | Hubrecht Institute |
| Country | Netherlands |
| Sector | Academic/University |
| PI Contribution | We provided tissue from transgenic mouse models |
| Collaborator Contribution | Collaborators will generate organoids for use in their research programme |
| Impact | None to date |
| Start Year | 2016 |
| Description | Heisler. CNS appetite circuitry |
| Organisation | University of Aberdeen |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Transgenic mouse models |
| Collaborator Contribution | Data analysis |
| Impact | Publication |
| Start Year | 2019 |
| Description | Holst perfused pancreas |
| Organisation | University of Copenhagen |
| Country | Denmark |
| Sector | Academic/University |
| PI Contribution | Ideas for experiments |
| Collaborator Contribution | Perfused pancreas to test ideas developed in Cambridge |
| Impact | Publication |
| Start Year | 2015 |
| Description | LGC peptidomics |
| Organisation | LGC Ltd |
| Country | Global |
| Sector | Private |
| PI Contribution | Supporting a BBSRC iCASE studentship |
| Collaborator Contribution | Analysis and training |
| Impact | One publication to date |
| Start Year | 2017 |
| Description | Larraufie Paris lab |
| Organisation | French National Institute of Agricultural Research |
| Country | France |
| Sector | Academic/University |
| PI Contribution | Experimentation |
| Collaborator Contribution | Experimentation and project planning |
| Impact | Publications |
| Start Year | 2016 |
| Description | Lilly GIPR |
| Organisation | Eli Lilly & Company Ltd |
| Country | United Kingdom |
| Sector | Private |
| PI Contribution | Research in the field of central GIPR signalling |
| Collaborator Contribution | Tool compounds and unpublished data |
| Impact | None yet |
| Start Year | 2018 |
| Description | Medimmune BBSRC iCASE (Galvin) |
| Organisation | AstraZeneca |
| Department | MedImmune |
| Country | United Kingdom |
| Sector | Private |
| PI Contribution | Supervision of PhD student |
| Collaborator Contribution | Joint supervision of student, training, peptide synthesis |
| Impact | None yet |
| Start Year | 2016 |
| Description | Mercodia assay validation |
| Organisation | Mercodia AB |
| Country | Sweden |
| Sector | Private |
| PI Contribution | Mass spec analysis to validate Mercodia's glucagon assay. Measurement of patient samples using glucagon and glicentin assays to establish the cause of assay cross reactivity |
| Collaborator Contribution | Provision of assays and suggestions for protocol changes to remove cross-reactivity |
| Impact | Mercodia published a white paper outlining the collaboration and how it has impacted on the use of the glucagon assay |
| Start Year | 2018 |
| Description | Moffett. Trophoblast physiology |
| Organisation | University of Cambridge |
| Department | Department of Pathology |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Application of LC-MS/MS peptidomics to analysis of trophoblast organoids |
| Collaborator Contribution | Development of trophoblast organoids |
| Impact | Publication |
| Start Year | 2018 |
| Description | Rinaman, Fadool, Williams. Central GLP-1 signalling |
| Organisation | Florida State University |
| Country | United States |
| Sector | Academic/University |
| PI Contribution | Provision of transgenic mouse models |
| Collaborator Contribution | Dissection of CNS appetite circuitry |
| Impact | multiple publications |
| Start Year | 2013 |
| Description | Ulster, transgenic mice |
| Organisation | Ulster University |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Collaboration involving transgenic mouse models generated in Cambridge, and shipped to Ulster |
| Collaborator Contribution | Collaborators will work with the transgenic model |
| Impact | None to date |
| Start Year | 2016 |
| Description | Valverde. PTP1B in GLP-1 secretion |
| Organisation | Autonomous University of Madrid |
| Country | Spain |
| Sector | Academic/University |
| PI Contribution | Hosted visiting student and provided training |
| Collaborator Contribution | Visiting student brought project |
| Impact | Publication |
| Start Year | 2017 |
| Description | Yuki Oka |
| Organisation | California Institute of Technology |
| Department | Division of Biology and Biological Engineering (BBE) |
| Country | United States |
| Sector | Academic/University |
| PI Contribution | Provision of mouse models |
| Collaborator Contribution | Research contribution |
| Impact | Nature publication |
| Start Year | 2017 |
| Title | GIP-Cre |
| Description | GIP-Cre genetically modified mouse model |
| IP Reference | |
| Protection | Protection not required |
| Year Protection Granted | |
| Licensed | No |
| Impact | MTAs in place for sharing with academic collaborators |
| Title | GLP1R Ab |
| Description | Joint development of anti-GLP1R antibody together with MedImmune |
| IP Reference | |
| Protection | Protection not required |
| Year Protection Granted | |
| Licensed | Yes |
| Impact | Sharing with academic groups |
| Description | 13th annual Biomarkers Congress |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Industry/Business |
| Results and Impact | An industrial congress bringing together a variety of industrial technologies for the measurement of biomarkers |
| Year(s) Of Engagement Activity | 2018 |
| Description | AEA ADA talk |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Alice Adriaenssens ADA 2021: invited talk for 'New turf for the other incretin' Diabetes journal symposium Follow up = invitation to contribute opinion piece in Diabetes, Feature article in 'ADA Meeting News' |
| Year(s) Of Engagement Activity | 2021 |
| Description | AEA Ascona Gut-Brain Axis Meeting, Switzerland |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Ascona Gut-Brain Axis Meeting, Switzerland. Collaborations and networking |
| Year(s) Of Engagement Activity | 2022 |
| Description | AEA Helmholtz Diabetes Meeting, Germany |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Helmholtz Diabetes Meeting, Germany. Networking and prize |
| Year(s) Of Engagement Activity | 2022 |
| Description | AEA Incretin 2022 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Good presentation. Interest in collaborations. Networking |
| Year(s) Of Engagement Activity | 2022 |
| Description | AEA Poster Presentation, Society for the Study of Ingestive Behaviour Annual Meeting, Portugal/Hybrid format |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Poster Presentation, Society for the Study of Ingestive Behaviour Annual Meeting, Portugal/Hybrid format. Networking and collaboration |
| Year(s) Of Engagement Activity | 2022 |
| Description | AEA SSIB talk |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | SSIB 2021: accepted talk Follow up = won Harry Kissileff prize for best talk, invitation to contribute to Appetite journal |
| Year(s) Of Engagement Activity | 2021 |
| Description | AEA. Oral presentation at Society for the Study of Ingestive Behaviour conference; Utrecht, Netherlands. 2019 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Research talk at Society for the Study of Ingestive Behaviour conference; Utrecht, Netherlands. |
| Year(s) Of Engagement Activity | 2019 |
| Description | AEA. Oral presentation at BSN Neuroendocrinology Celebrate Symposium for ECRs; Glasgow, UK |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | Invited talk at BSN Neuroendocrinology Celebrate Symposium for Early Career Researchers; Glasgow, UK |
| Year(s) Of Engagement Activity | 2019 |
| Description | AEA. Oral presentation at Experimental Biology conference; Orlando, US. 2019 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Invited speaker at Experimental Biology conference; Orlando, US. Networking |
| Year(s) Of Engagement Activity | 2019 |
| Description | AEA. Oral presentation to Eli Lilly; Indianapolis, US, 2019 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | Research presentation to Eli Lilly. |
| Year(s) Of Engagement Activity | 2019 |
| Description | AEA. Poster at EASD Incretin Study Group |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | AEA Poster at Incretin Study Group "The role of glucose-dependent insulinotropic polypeptide receptor-expressing neurons in the hypothalamus" |
| Year(s) Of Engagement Activity | 2019 |
| Description | AEA. Poster at NovoNordisk Foundation Metabolism in Action conference; Copenhagen, Denmark 2019 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Poster presentation at Novonordisk Foundation Metabolism in Action conference; Copenhagen, Denmark. Sparked debate. |
| Year(s) Of Engagement Activity | 2019 |
| Description | Big Biology Day |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Schools |
| Results and Impact | Big Biology Day - engagement activities explaining metabolic research to families |
| Year(s) Of Engagement Activity | 2017 |
| Description | CAS. Poster at EASD incretin study group 2019 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | CAS. Poster at EASD incretin study group: "Tracking the development and release of hormone-containing vesicles in murine L-cells" |
| Year(s) Of Engagement Activity | 2019 |
| Description | Cambridge City Council holiday club EM |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Public/other audiences |
| Results and Impact | Science activities at Cambridge City Council drop-in holiday club for families |
| Year(s) Of Engagement Activity | 2021 |
| Description | Cambridge Science Festival - family activities |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Public/other audiences |
| Results and Impact | Drop-in hands-on event for families and children. Sparked questions from children and parents |
| Year(s) Of Engagement Activity | 2016,2017,2018 |
| Description | Cambridge Science festival, family weekends |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Public/other audiences |
| Results and Impact | promoted interest in science taking place at the inst |
| Year(s) Of Engagement Activity | 2013,2014,2015 |
| Description | Cambridgeshire Girlguiding Girls in STEM event |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Other audiences |
| Results and Impact | Cambridgeshire Girlguiding Girls in STEM event |
| Year(s) Of Engagement Activity | 2020 |
| Description | DG Poster at EASD study group 2019 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | DG. Poster at Incretin Study Group: "Modelling human enteroendocrine cells using adult intestinal organoids" |
| Year(s) Of Engagement Activity | 2019 |
| Description | DG. Poster at Copenhagen Biosciences Conference on Intestinal Organoids 2019 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | "Modelling hormone secretion in human enteroendocrine cells using adult intestinal organoids" |
| Year(s) Of Engagement Activity | 2019 |
| Description | EB. Poster at Academic Foundation Programme and NIHR BRC and BTRU Trainee's Annual Research Day 2018 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Professional Practitioners |
| Results and Impact | "Localisation and characterisation of glucose-dependent insulinotropic polypeptide receptor (GIPR) expressing cells" |
| Year(s) Of Engagement Activity | 2018 |
| Description | EM Talk at Society for Endocrinology conference |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | Talk at Society for Endocrinology conference |
| Year(s) Of Engagement Activity | 2021 |
| Description | EM. Poster at Copenhagen Bioscience Conference on Intestinal Organoids 2019 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | EM Poster. "Using human duodenal organoids to study motilin production and secretion" |
| Year(s) Of Engagement Activity | 2019 |
| Description | EM. Poster at EASD incretin study group 2019 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | EM. Poster at EASD incretin study group. "The contribution of TRP channels to L-cell signalling" |
| Year(s) Of Engagement Activity | 2019 |
| Description | FG Foster talk |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Postgraduate students |
| Results and Impact | FG gave invited lecture in the Department of Physiology, Development and Neuroscience at University of Cambridge. |
| Year(s) Of Engagement Activity | 2021 |
| Description | FG invited speaker at Keystone conference: Interconnection between the Gut, Brain and Microbiome for Metabolic Disease |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Triggered questions, collaborations and discussion with industry |
| Year(s) Of Engagement Activity | 2022 |
| Description | FG invited speaker at Metabolism in Action conference in Copenhagen |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Triggered questions and potential collaborations |
| Year(s) Of Engagement Activity | 2022 |
| URL | https://cbmr.ku.dk/metabolism-in-action/ |
| Description | FG invited speaker at symposium on 100 years of Glucagon |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Invited talk at this symposium |
| Year(s) Of Engagement Activity | 2022 |
| URL | http://glucagonconference.com/ |
| Description | FG invited talk at 7th International Conference on Food Digestion, Cork |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | Invited speaker at this conference on food digestion. |
| Year(s) Of Engagement Activity | 2022 |
| URL | http://www.icfd2022.com |
| Description | FG invited talk at International scientific symposium - Gut / brain axis and regulation of food intake, organized by SFNCM/INRAE |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | Invited talk at this symposium. Delivered virtually due to travel difficulties. Inspired questions from the audience |
| Year(s) Of Engagement Activity | 2022 |
| Description | FG talk at MRC-DTP symposium |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Postgraduate students |
| Results and Impact | FG gave invited lecture at the annual symposium of the MRC-DTP students in Cambridge. |
| Year(s) Of Engagement Activity | 2021 |
| Description | FG talk at Milner Therapeutics Institute |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | FG gave invited lecture to the Milner Institute, which includes industrial audiences from around the world. The talk triggered many interesting questions. |
| Year(s) Of Engagement Activity | 2021 |
| Description | FR SSIB annual meeting 2019 - poster presentation |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | SSIB annual meeting 09-13Jul2019 (poster): "Classification of colonic enteroendocrine cells based on single cell mRNA expression analysis" |
| Year(s) Of Engagement Activity | 2019 |
| Description | FR oral presentation at EASD annual meeting 2018 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | oral presentation "GLP-1 secreting enteroendocrine cells co-release ATP to modulate vagal afferent neuronal activity" delivered on 05Oct2018 |
| Year(s) Of Engagement Activity | 2018 |
| Description | FR oral presentation at Wellcome Trust meeting |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Wellcome Meeting at Sanger 31Jan-01Feb2019 (oral): "A role of hypothalamic glucose-dependent insulinotropic polypeptide receptor (GIPR) in feeding behaviour" |
| Year(s) Of Engagement Activity | 2019 |
| Description | FR. Poster at EASD incretin study group. |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | FR poster at EASD study group: "Classification of colonic enteroendocrine cells based on single cell mRNA expression analysis" |
| Year(s) Of Engagement Activity | 2019 |
| Description | FR. presentation at Danish Diabetes Academy Winter School for postdoctoral researchers, at Higuerõn Hotel in Malaga/Spain 2019 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | "Incretins along the gut-pancreas-brain axis". |
| Year(s) Of Engagement Activity | 2019 |
| Description | FR: Mercodia Diagnostics, Uppsala. Invited speaker |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | Invited to talk to Mercodia about how our mass spec analysis methods complement their immunoassay development |
| Year(s) Of Engagement Activity | 2019 |
| Description | GPR. Cambridge UGI cancer symposium |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Professional Practitioners |
| Results and Impact | "The post-gastrectomy patient, metabolic considerations" |
| Year(s) Of Engagement Activity | 2018 |
| Description | IMS online academy EM CAS OW |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | developed work experience programme for ~12 Y10 students from underrepresented schools in Cambridge |
| Year(s) Of Engagement Activity | 2021 |
| Description | JL Choosing your first postdoc. |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Postgraduate students |
| Results and Impact | Lewis JE and Joanisse D (2021) Choosing your first postdoc. Career Progression Workshop, Future Physiology, online. |
| Year(s) Of Engagement Activity | 2021 |
| Description | JL Oral presentation at BSN/SFE, online |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | Lewis JE, Woodward ORM, Phillips BU, Tripodi M, Hornigold D, Baker D, Gribble FM, Reimann F (2021) Relaxin/insulin-like family peptide receptor 4 (Rxfp4) expressing hypothalamic neurons modulate food intake and preference in mice. |
| Year(s) Of Engagement Activity | 2021 |
| Description | JL. Poster at EASD incretin study group 2019 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | JL. Poster at EASD study group. "RXFP4, the cognate receptor for insulin-like peptide 5, alters food preference in mice" |
| Year(s) Of Engagement Activity | 2019 |
| Description | MRL Science Slam |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Public/other audiences |
| Results and Impact | The presenters (a post doc and student together) won a prize for their presentation |
| Year(s) Of Engagement Activity | 2018 |
| Description | MY Poster at Incretin Study Group 2019 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | MY poster at Incretin Study Group: "Amino acid sensing in K and L cells from mouse duodenal organoids" |
| Year(s) Of Engagement Activity | 2019 |
| Description | MY. Oral presentation at Diabetes UK Annual Professional Conference 2019 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | Metformin alters glucose uptake and metabolism in intestinal cells |
| Year(s) Of Engagement Activity | 2019 |
| Description | OW STEM for BRITAIN |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Policymakers/politicians |
| Results and Impact | STEM for BRITAIN is a major scientific poster competition and exhibition which has been held in Parliament since 1997, and is organised by the Parliamentary & Scientific Committee. Its aim is to give members of both Houses of Parliament an insight into the outstanding research work being undertaken in UK universities by early-career researchers. Prizes are awarded for the posters presented in each discipline which best communicated high level science, engineering or mathematics to a lay audience. The audience was made up of MPs, senior scientists, industry representatives and early career researchers. |
| Year(s) Of Engagement Activity | 2022 |
| URL | https://stemforbritain.org.uk/ |
| Description | OW. Poster at EASD incretin study group 2019 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | OW poster at EASD incretin study group: "Distribution of G protein coupled relaxin/insulin-like family peptide receptor-4 (RXFP4) expressing cells in the mouse brain" |
| Year(s) Of Engagement Activity | 2019 |
| Description | PL. Talk at Incretin Study Group 2019 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | PL. Talk at Incretin Study Group. "Peptidomics to study enteroendocrine cells after bariatric surgery" |
| Year(s) Of Engagement Activity | 2019 |
| Description | REF Poster at SymBLS 2019 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Postgraduate students |
| Results and Impact | Size Exclusion Chromatography for Measuring Free and Antibody-bound Insulin in Hiratas Patients |
| Year(s) Of Engagement Activity | 2019 |
| Description | REF. Poster at DMDG open meeting 2019 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Industry/Business |
| Results and Impact | "Size Exclusion Chromatography for Measuring Free and Antibody-bound Insulin in Hiratas Patients" |
| Year(s) Of Engagement Activity | 2019 |
| Description | REF. Poster at EASD incretin study group 2019 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | REF poster at EASD study group "A multiplex method for quantifying gut hormone peptides using Mass Spectrometry" |
| Year(s) Of Engagement Activity | 2019 |
| Description | REF. Poster at REID Bioanalytical Forum 2019 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Industry/Business |
| Results and Impact | Immunoassay vs Mass Spectrometry -Measuring insulin and insulin analogues in human plasma |
| Year(s) Of Engagement Activity | 2019 |
| Description | RGK Incretin 2022 |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | poster presentation at Incretin 2022. Lots of interest |
| Year(s) Of Engagement Activity | 2022 |
| Description | RGK Invited lecture on Mass Spectrometric analysis of Insulin at Anglian Ruskin University. Cambridge, UK |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Undergraduate students |
| Results and Impact | 1st March 2023. Invited lecture on Mass Spectrometric analysis of Insulin at Anglian Ruskin University. Cambridge, UK Audience: Staff and students Size: ~100 participants |
| Year(s) Of Engagement Activity | 2022 |
| Description | RGK Invited speaker at Reid Bioanalytical Forum. Cambourne, Cambridgeshire, UK |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Industry/Business |
| Results and Impact | 5Th September 2022. Invited speaker at Reid Bioanalytical Forum. Cambourne, Cambridgeshire, UK Audience: Mainly bioanalytical scientists from industry Size: ~100 participants |
| Year(s) Of Engagement Activity | 2022 |
| Description | RGK Invited speaker at the Peptidomics Symposium. University of Copenhagen, Denmark |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | 23rd August 2022. Invited speaker at the Peptidomics Symposium. University of Copenhagen, Denmark Audience: Academics and Clinicians Size: ~150 participants Outcome: Was invited to talk at the 2023 Danish Diabetes and Endocrine Academy conference |
| Year(s) Of Engagement Activity | 2022 |
| Description | RGK London Proteomics Discussion Group. London, UK |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | Audience: Academic and industry researchers in the proteomics field Size: ~50 participants Outcome: Was invited to talk at the 2022 Reid Bioanalytical Forum |
| Year(s) Of Engagement Activity | 2022 |
| Description | RGK. American Society for Mass Spectrometry (ASMS) 2019 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Industry/Business |
| Results and Impact | Waters user group meeting, presentation: "LC-MS analysis of plasma from patients with insulin autoimmune syndrome" Waters MS focused user meeting (Pharmaceutical track) Outcome: Video of talk available on Waters website, video interview on Select Science website, as well as an in-lab video interview. |
| Year(s) Of Engagement Activity | 2019 |
| Description | RGK. Poster at EASD incretin study group 2019 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Poster at EASD incretin study group: "Proglucagon peptide analysis - will the real peptide please stand up!" |
| Year(s) Of Engagement Activity | 2019 |
| Description | RGK. Reid Bioanalytical Forum 2019 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Industry/Business |
| Results and Impact | "LC-MS analysis of plasma from patients with insulin autoimmune syndrome" Meeting focussing on broad bioanalytical approaches for wide range of analytes Outcome: Video interview by Bionalaysis zone, available on their website |
| Year(s) Of Engagement Activity | 2019 |
| Description | RGK. Waters UK and Ireland Clinical User Meeting 2019 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Industry/Business |
| Results and Impact | Waters UK and Ireland Clinical User Meeting 2019 "LCMS Analysis of Proinsulin Derived Peptides" Waters meeting for users of MS systems in clinical environments |
| Year(s) Of Engagement Activity | 2019 |
| Description | SG. Poster at EASD incretin study group |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | SG. Poster at EASD study group. "Characterisation of novel gut-derived peptides in vivo and in vitro" |
| Year(s) Of Engagement Activity | 2019 |
| Description | Suffolk Science Festival |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Public/other audiences |
| Results and Impact | Suffolk Science Festival |
| Year(s) Of Engagement Activity | 2020 |
| Description | TD. Poster at EASD study group 2019 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | TD. Poster at EASD incretin study group. "Neutral amino acid transporter: SLC6a19- linking amino acid sensing to gut hormone secretion?" |
| Year(s) Of Engagement Activity | 2019 |
| Description | VBL talk at Incretin 2019 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | VBL talk at EASD Incretin Study group, "Adenosine triphosphate (ATP) is released from glucagon-like peptide 1 (GLP-1) secreting cells and signals to vagal afferent neurons" |
| Year(s) Of Engagement Activity | 2019 |
| Description | VBL. Poster at Cambridge Neuroscience Seminar 2019: Signalling, Sensation & Sentience |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Professional Practitioners |
| Results and Impact | VBL presented a poster at a local Cambridge Neuroscience Association meeting at Robinson College on March 14th, 2019. It was called "Cambridge Neuroscience Seminar 2019: Signalling, Sensation & Sentience" and the poster title was "ATP is released from GLP-1 secreting enteroendocrine cells and signals to vagal afferent neruones". It was a valuable experience sharing our work on gut-brain signalling to the larger Cambridge neuroscience community. |
| Year(s) Of Engagement Activity | 2019 |