Single-molecule super-resolution microscopy of membrane dynamics: application to immunology
Lead Research Organisation:
University of Oxford
Abstract
Research by my group aims to unravel nanoscopic changes at the molecular level in living cells to characterise important molecular processes on the cell membrane as well as inside the cell during immunological reactions. Because many cellular responses lead to changes so subtle at the molecular level, studying them requires us to not only observe them with a superior spatial resolution but also to reach a sensitivity that is able to monitor single molecules over time and space. We are using the newest and most powerful super-resolution far-field microscopes (such as STED, RESOLFT or PALM/STORM) to image and analyse cellular structures and protein-protein and protein-lipid interactions at a level of fine detail that until now has not been possible due to the limited spatial resolution of conventional optical far-field microscopes. By combining these super-resolution microscopy techniques with single-molecule sensitive detection methods (such as fluorescence correlation spectroscopy) and fast spatio-temporal tracking tools we are able to see complex dynamic processes otherwise invisible because of the lower power of conventional far-field microscopy. These molecular interactions play an important role in the immune response to infection and cancer and so we intend to use and further develop these advanced microscopy techniques and apply them to gather new insights in immunological research.
Technical Summary
Single-molecule super-resolution microscopy of membrane dynamics: Many cellular responses lead to subtle changes on the molecular level, demanding not only for a superior spatial resolution of the analyzing method but also for the sensitivity to monitor single molecules over time and space. The combination of super-resolution optical fluorescence STED microscopy with single-molecule sensitive fluorescence-detection tools such as Fluorescence Correlation Spectroscopy (FCS) as well as the fast spatio-temporal tracking of single labeled molecules (single-particle tracking, SPT) allows for the disclosure of complex dynamic processes otherwise impeded by the limited spatial resolution of conventional far-field microscopy. For example, STED-FCS or SPT offer us the possibility to gain novel insights into important cellular processes, such as lipid-lipid, lipid-protein, and protein-protein interactions and the formation of so-called “lipid-rafts” in the cellular plasma membrane. These molecular interactions play an important role in the cellular immune response. We will therefore apply and further develop the STED-FCS and SPT microscopy techniques to highlight important molecular processes on the plasma membrane as well as inside the cell during immunological reactions. For example, these techniques will be used to shed new light on different molecular pathways triggered at the cell surface and intracellularly during antigen presentation by dendritic cells and T cell activation.
Organisations
- University of Oxford, United Kingdom (Collaboration, Lead Research Organisation)
- Max Planck Society (Collaboration)
- Swiss Federal Institute of Technology in Lausanne (EPFL) (Collaboration)
- University of Warwick, United Kingdom (Collaboration)
- Royal Institute of Technology (Collaboration)
- University of Goettingen, Germany (Collaboration)
- University of Bonn, Germany (Collaboration)
- Chalmers University of Technology, Sweden (Collaboration)
- University of Birmingham, United Kingdom (Collaboration)
- University of Manchester, Manchester, United Kingdom (Collaboration)
People |
ORCID iD |
| Christian Eggeling (Principal Investigator) |
Publications
Amaro M
(2017)
Laurdan and Di-4-ANEPPDHQ probe different properties of the membrane.
in Journal of physics D: Applied physics
Ando T
(2018)
The 2018 correlative microscopy techniques roadmap.
in Journal of physics D: Applied physics
Andrade DM
(2015)
Cortical actin networks induce spatio-temporal confinement of phospholipids in the plasma membrane--a minimally invasive investigation by STED-FCS.
in Scientific reports
Bally M
(2012)
Norovirus GII.4 virus-like particles recognize galactosylceramides in domains of planar supported lipid bilayers.
in Angewandte Chemie (International ed. in English)
Basu S
(2018)
FRET-enhanced photostability allows improved single-molecule tracking of proteins and protein complexes in live mammalian cells.
in Nature communications
Bernardino De La Serna J
(2016)
There Is No Simple Model of the Plasma Membrane Organization.
in Frontiers in cell and developmental biology
Carravilla P
(2019)
Molecular recognition of the native HIV-1 MPER revealed by STED microscopy of single virions.
in Nature communications
Carugo D
(2017)
Modulation of the molecular arrangement in artificial and biological membranes by phospholipid-shelled microbubbles.
in Biomaterials
Chmyrov A
(2013)
Nanoscopy with more than 100,000 'doughnuts'.
in Nature methods
| Description | Access to the Advanced Imaging Facility at Janelia, Howard Hughes Medical Institute Janelia, entitled: Cytoskeleton induced microcluster dynamics lead to T-cell synapse formation |
| Amount | $1,000 (USD) |
| Organisation | Howard Hughes Medical Institute |
| Department | Janelia Research Campus |
| Sector | Academic/University |
| Country | United States |
| Start | 11/2014 |
| End | 12/2014 |
| Description | Access to the Central Laser Facility, STFC Rutherford Appleton Laboratory, Harwell Oxford Didcot, UK, Advanced single-molecule tracking in immune cells at the Weatherall Institute of Molecular Medicine |
| Amount | £1 (GBP) |
| Organisation | Rutherford Appleton Laboratory |
| Sector | Public |
| Country | United Kingdom |
| Start | 07/2014 |
| End | 08/2016 |
| Description | Advancing Image Analysis at the Wolfson Imaging Centre Oxford |
| Amount | £45,000 (GBP) |
| Organisation | University of Oxford |
| Department | E P A Cephalosporin Fund |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 09/2016 |
| End | 04/2018 |
| Description | BBSRC research grant (BB/L00433X/1) Dynamics of Peroxisomal Protein Transport |
| Amount | £167,308 (GBP) |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 01/2014 |
| End | 12/2016 |
| Description | Dynamics of Peroxisomal Protein Transport |
| Amount | £1,730,400 (GBP) |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 05/2013 |
| End | 04/2016 |
| Description | Dynamics of Peroxisomal Protein Transport National Research Unit FOR1905-PerTrans |
| Amount | € 207,800 (EUR) |
| Organisation | German Research Foundation |
| Sector | Public |
| Country | Germany |
| Start | 01/2017 |
| End | 12/2019 |
| Description | EMBO Long-Term Fellowship (contribution to group member Erdinc Sezgin) |
| Amount | £26,000 (GBP) |
| Organisation | European Molecular Biology Organisation |
| Sector | Learned Society |
| Country | European Union (EU) |
| Start | 01/2014 |
| End | 12/2015 |
| Description | EP Abraham Cephalosporin Trust Fund 2014 Nanoscopy Oxford: Super-resolution optical STED microscope facility at the WIMM |
| Amount | £62,000 (GBP) |
| Organisation | University of Oxford |
| Department | E P A Cephalosporin Fund |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 06/2014 |
| End | 04/2016 |
| Description | EP Abraham Cephalosporin Trust Fund 2015 BioISCAT: Biomedical application of novel fast molecular tracking. Partially funded. £ |
| Amount | £74,200 (GBP) |
| Organisation | University of Oxford |
| Department | E P A Cephalosporin Fund |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 07/2015 |
| End | 07/2016 |
| Description | EPSRC/MRC Oxford-Nottingham Centre for Doctoral Training in Biomedical Imaging. Member of the management board. 15 students per year across all imaging modalities, developing and applying novel image analysis and imaging hardware approaches |
| Amount | £1 (GBP) |
| Organisation | University of Leeds |
| Department | Faculty of Engineering |
| Sector | Academic/University |
| Country | United Kingdom |
| Start | 10/2014 |
| End | 10/2020 |
| Description | Enabling enhanced optical imaging of whole organs by optimized tissue clearing apparatus at University of Oxford light microscopy imaging facilities |
| Amount | £15,000 (GBP) |
| Organisation | University of Oxford |
| Department | E P A Cephalosporin Fund |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 01/2017 |
| End | 01/2018 |
| Description | Establishment of force measurements in biomedical research |
| Amount | £57,500 (GBP) |
| Organisation | University of Oxford |
| Department | John Fell Fund |
| Sector | Academic/University |
| Country | United Kingdom |
| Start | 06/2016 |
| End | 05/2017 |
| Description | John Fell OUP Research Fund Matching funds for external applications for acquisition of a Zeiss 880 upright confocal microscope with novel Airy-scan super-resolution detector |
| Amount | £100,000 (GBP) |
| Organisation | University of Oxford |
| Department | John Fell Fund |
| Sector | Academic/University |
| Country | United Kingdom |
| Start | 06/2015 |
| End | 12/2016 |
| Description | MRC project grant (MR/L018942/1): The impact of IFTIM3 genetic variation on Influenza virus infection, immune responses and disease outcome |
| Amount | £200,000 (GBP) |
| Funding ID | MR/L018942/1 |
| Organisation | Medical Research Council (MRC) |
| Sector | Academic/University |
| Country | United Kingdom |
| Start | 03/2014 |
| End | 02/2017 |
| Description | MRC/BBSRC/EPSRC Next generation imaging grant (MR/K01577X/1). |
| Amount | £2,000,000 (GBP) |
| Organisation | Medical Research Council (MRC) |
| Sector | Academic/University |
| Country | United Kingdom |
| Start | 04/2013 |
| End | 03/2018 |
| Description | Marie Curie Career Integration Fund (contribution to group member Jorge Bernardino de la Serna) |
| Amount | € 100,000 (EUR) |
| Organisation | Marie Sklodowska-Curie Actions |
| Sector | Academic/University |
| Country | Global |
| Start | 10/2013 |
| End | 09/2017 |
| Description | Marie Curie Intra-European Fellowship (Erdinc Sezgin) |
| Amount | € 221,606 (EUR) |
| Organisation | Marie Sklodowska-Curie Actions |
| Sector | Academic/University |
| Country | Global |
| Start | 01/2015 |
| End | 12/2016 |
| Description | Marie-Curie Fellowship (contribution to group member Iztok Urbancic) |
| Amount | £164,000 (GBP) |
| Organisation | Marie-Sklodowska University |
| Sector | Academic/University |
| Country | Poland |
| Start | 09/2016 |
| End | 08/2019 |
| Description | Newton Fund Researcher Links Workshop Grant 2015 |
| Amount | £30,000 (GBP) |
| Organisation | British Council |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 11/2015 |
| End | 05/2016 |
| Description | Newton Fund Researcher Links Workshop Grant 2016 |
| Amount | £30,000 (GBP) |
| Organisation | British Council |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 04/2017 |
| End | 12/2017 |
| Description | Newton Institutional Links Fund (Turkey/UK): Investigation of Wnt-catenin signalling at the plasma membrane in health and disease |
| Amount | £340,000 (GBP) |
| Organisation | British Council |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 02/2018 |
| End | 01/2020 |
| Description | Oxford Medical Sciences Internal Bridging Salary Fund Silvia Galiani |
| Amount | £7,332 (GBP) |
| Organisation | University of Oxford |
| Department | Medical Sciences Division |
| Sector | Academic/University |
| Country | United Kingdom |
| Start | 01/2017 |
| End | 03/2017 |
| Description | Wellcome Trust Enhancement of Strategic Award (WT 091911) Advanced Microscopy for Chromosome and RNA dynamics |
| Amount | £178,795 (GBP) |
| Organisation | Wellcome Trust |
| Department | Wellcome Trust Strategic Award |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 05/2014 |
| End | 12/2015 |
| Description | Wellcome Trust Institutional Strategic Support Fund (WTISSF) 2013-2014 Containment level 3 super-resolution STED imaging of live virus host-cell interactions £ |
| Amount | £70,000 (GBP) |
| Organisation | Wellcome Trust |
| Department | Wellcome Trust Strategic Award |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 01/2014 |
| End | 12/2015 |
| Description | Wellcome Trust Institutional Strategic Support Fund (WTISSF) Enhancement of technological capacity in biomedical research: Wolfson Imaging Centre Oxford |
| Amount | £101,789 (GBP) |
| Organisation | Wellcome Trust |
| Department | Wellcome Trust Strategic Award |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 06/2015 |
| End | 05/2017 |
| Description | Wellcome Trust Multi-User Equipment Grant (WT104924AIA) Advanced super-resolution fluorescence STED microscopy of the cellular interior |
| Amount | £389,000 (GBP) |
| Organisation | Wellcome Trust |
| Department | Wellcome Trust Strategic Award |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 01/2014 |
| End | 12/2018 |
| Description | Wellcome Trust Strategic Award Micron Oxford: super-resolution imaging of cellular dynamics |
| Amount | £4,679,473 (GBP) |
| Organisation | Wellcome Trust |
| Department | Wellcome Trust Strategic Award |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 01/2016 |
| End | 12/2021 |
| Description | Advancing fluorescence microscopy |
| Organisation | University of Oxford |
| Department | Micron Oxford Advanced Bioimaging Unit |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Fluorescence microscopy development and servicing |
| Collaborator Contribution | Complementary fluorescence microscopy development and servicing |
| Impact | One publication, three major grants, ongoing technological and infrastructural development, positive evaluation as a Eurobioimaging node |
| Start Year | 2013 |
| Description | Antigen Presenting Cells |
| Organisation | University of Oxford |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Fluorescence microscopy, biophysical techniques, data analysis |
| Collaborator Contribution | APC biology |
| Impact | DPhil studentship |
| Start Year | 2014 |
| Description | Bcell membrane antibody interaction |
| Organisation | University of Manchester |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Fluorescence microscopy |
| Collaborator Contribution | Cells, antibody |
| Impact | Ongoing |
| Start Year | 2015 |
| Description | BioiScat - molecular membrane dynamics |
| Organisation | University of Oxford |
| Department | Department of Chemistry |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Setup and application of iSCAT setup - study of molecular membrane dynamics |
| Collaborator Contribution | Setup of iSCAT setup, data analysis |
| Impact | Two publications EPA grant DPhil student |
| Start Year | 2014 |
| Description | Data analysis |
| Organisation | University of Warwick |
| Department | Warwick Systems Biology Centre |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Supply of experimental data for analysis |
| Collaborator Contribution | Data analysis of experimental data |
| Impact | Studentship |
| Start Year | 2010 |
| Description | Engineering |
| Organisation | University of Oxford |
| Department | Department of Engineering Science |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Application and development of 3D super-resolution microscopy |
| Collaborator Contribution | Development of 3D super-resolution microscopy |
| Impact | Project planning |
| Start Year | 2012 |
| Description | Epidermal tissue development |
| Organisation | University of Oxford |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Fluorescence microscopy, data analysis |
| Collaborator Contribution | skin cell biology |
| Impact | One publication close to submission |
| Start Year | 2013 |
| Description | Fluorescence Correlation Spectroscopy |
| Organisation | Royal Institute of Technology |
| Country | Sweden |
| Sector | Academic/University |
| PI Contribution | Fluorescence Correlation Spectroscopy and photophysics: Experiments and Discussions |
| Collaborator Contribution | Fluorescence Correlation Spectroscopy and photophysics: Experiments and Discussions |
| Impact | Several publications. |
| Description | Fluorescent lipid analog development |
| Organisation | University of Birmingham |
| Department | School of Chemistry |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Fluorescence microscopy |
| Collaborator Contribution | Lipid synthesis and delivery |
| Impact | One publication in preparation |
| Start Year | 2014 |
| Description | Labels for super-resolution microscopy |
| Organisation | University of Göttingen |
| Country | Germany |
| Sector | Academic/University |
| PI Contribution | Application and development of labels for super-resolution microscopy, |
| Collaborator Contribution | Development of Labels for super-resolution microscopy |
| Impact | Two Nature papers |
| Description | Lipid rafts |
| Organisation | Max Planck Society |
| Department | Max Planck Institute for Molecular Cell Biology and Genetics |
| Country | Germany |
| Sector | Public |
| PI Contribution | Super-resolution experiments on proteins and lipids in membranes. |
| Collaborator Contribution | Delivery of protein constructs and lipids. Know-how transfer on lipid rafts. Workshops. |
| Impact | One publication. Design of experiments. |
| Start Year | 2009 |
| Description | Lipid synthesis |
| Organisation | University of Bonn |
| Country | Germany |
| Sector | Academic/University |
| PI Contribution | Measurements and characterization of synthesized fluorescent lipid analogs |
| Collaborator Contribution | Synthesis of fluorescent lipid analogs |
| Impact | Several publications including a Nature paper. |
| Start Year | 2008 |
| Description | Live-cell labeling |
| Organisation | Swiss Federal Institute of Technology in Lausanne (EPFL) |
| Department | Institute of Chemical Sciences and Engineering |
| Country | Switzerland |
| Sector | Academic/University |
| PI Contribution | Microscope experiments |
| Collaborator Contribution | Development of live-cell fluorescent labels |
| Impact | Publication in Nature Chemistry |
| Start Year | 2011 |
| Description | Lymphatic cell receptor organization |
| Organisation | University of Oxford |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Fluorescence microscopy, data analysis |
| Collaborator Contribution | Lymphatic Receptor Biology |
| Impact | One publication, one DPhil studentship |
| Start Year | 2013 |
| Description | Micron |
| Organisation | University of Oxford |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Exchange of microscopy knowhow. |
| Collaborator Contribution | Exchange of microscopy know-how. |
| Impact | Design of imaging facility. NanOxford imaging initiative. Further developments in super-resolution microscopy. |
| Start Year | 2012 |
| Description | Photoswitchable label development |
| Organisation | University of Oxford |
| Department | Department of Chemistry |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Fluorescence microscopy |
| Collaborator Contribution | Organic chemistry, organic dye synthesis |
| Impact | One publication two grants |
| Start Year | 2014 |
| Description | Physics |
| Organisation | University of Oxford |
| Department | Department of Physics |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Applications and developments of microscopy. |
| Collaborator Contribution | Sample preparations and analysis software for microscopy |
| Impact | Experimental design of experiments. Partnership with physics department. |
| Start Year | 2012 |
| Description | STED/RESOLFT microscopy |
| Organisation | Max Planck Society |
| Department | Max Planck Institute for Biophysical Chemistry Goettingen |
| Country | Germany |
| Sector | Public |
| PI Contribution | Application of super-resolution STED/RESOLFT microscopy |
| Collaborator Contribution | Development of super-resolution STED/RESOLFT microscopy |
| Impact | Design of setups/microscopes Several publications on developments in STED/RESOLFT microscopy |
| Start Year | 2012 |
| Description | T-cell activation |
| Organisation | University of Oxford |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Fluorescence microscopy of T-cell during activation, receptor and actin dynamics - our contribution microscopy and analysis |
| Collaborator Contribution | T-cell biology |
| Impact | 3 publications, one Wellcome Trust Grant |
| Start Year | 2013 |
| Description | Tracking |
| Organisation | University of Oxford |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Super-resolution measurements on membrane dynamics. |
| Collaborator Contribution | Single-molecule tracking experiments of membrane dynamics. |
| Impact | Verification of experimental results. |
| Start Year | 2011 |
| Description | Viral entry |
| Organisation | Chalmers University of Technology |
| Country | Sweden |
| Sector | Academic/University |
| PI Contribution | Delivery of lipids, discussion of data/experiments |
| Collaborator Contribution | Substances for experiments |
| Impact | Publication in Angewandte Int Ed |
| Start Year | 2011 |
| Title | CalQuo - quantifying cellular calcium release |
| Description | Development of a software to quantify calcium release from an ensemble of cells on a single-cell level |
| IP Reference | |
| Protection | Copyrighted (e.g. software) |
| Year Protection Granted | 2015 |
| Licensed | No |
| Impact | Advances general data analysis |
| Description | Articles Written for the Leica Science Lab |
| Form Of Engagement Activity | A magazine, newsletter or online publication |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Added information to Leica website about nanoscopy. have received queries about further information no notable impact |
| Year(s) Of Engagement Activity | 2012,2013,2014,2015,2016 |
| URL | http://www.leica-microsystems.com/science-lab/ |
| Description | Conference Organizations |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Postgraduate students |
| Results and Impact | Conference/workshop Organizer: 4th International Symposium on Physics, Chemistry & Biology with Single Molecules, German Volkswagen foundation, Kloster Banz, Germany (2004); The Physics of Imaging, German Physical Society, Bad Honnef, Germany (2005); Trends in Biological Optical Microscopy, Boehringer Ingelheim foundation, Titisee, Germany (2007); SPIE Photonics West BIOS, Single Molecule Spectroscopy and Superresolution Imaging, USA (2009-); SPIE Nanoimaging and Nanospectroscopy, San Diego (2013-); EOS Frontiers in Optical Imaging, Murten, Switzerland (2013); Leica Super-Resolution User Club Meeting, Oxford, UK (2014); Coherent Bioimaging Workshop, Oxford, UK (2014); Conference on Lasers and Electro-Optics Europe (CLEO/Europe), Munich, Germany (2015); International Conference On Nanoscopy EUROPE (ICON2016), Basel, 2016; EBSA European Biophysical Congress, Madrid, 2019 |
| Year(s) Of Engagement Activity | 2012,2013,2014,2015,2016 |
| Description | Editorial Board Member |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Media (as a channel to the public) |
| Results and Impact | Editorial Board Member in journals: NanoBioimaging, Frontiers in Physiology, Journal of Physics D, Scientific Reports |
| Year(s) Of Engagement Activity | 2012,2013,2014,2015,2016 |
| Description | High School, undergraduate and graduate placement students in lab |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Schools |
| Results and Impact | Visits of high school, undergraduate and graduate students (7 so far) in my lab for up to 6 months, teaching them in our research |
| Year(s) Of Engagement Activity | 2013,2014,2015,2016 |
| Description | High-School visits |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | Visits of high school students to our institute/laboratory - insights into research activities |
| Year(s) Of Engagement Activity | 2017 |
| Description | Lectures |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Type Of Presentation | Keynote/Invited Speaker |
| Geographic Reach | International |
| Primary Audience | Postgraduate students |
| Results and Impact | Insights into general and super-resolution fluorescence microscopy and membrane bioactivity Increased enquiry in using our imaging facilities, teach students/researchers |
| Year(s) Of Engagement Activity | 2012,2013,2014,2015,2016 |
| Description | Microscopy Teaching |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Public/other audiences |
| Results and Impact | Teaching on microscopy on international/national fares, workshops, University courses, doctoral and undergraduate training centres |
| Year(s) Of Engagement Activity | 2012,2013,2014,2015,2016 |
| Description | Microscopy Teaching in High Schools |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Schools |
| Results and Impact | Teach basics of microscopy to high school kids including hands-on |
| Year(s) Of Engagement Activity | 2015,2016 |
| Description | Microscopy Workshop Izmir, Turkey 10.2017 |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Undergraduate students |
| Results and Impact | Teaching of basics of optical microscopy to public/scientists in Turkey - funded by the British Council |
| Year(s) Of Engagement Activity | 2017 |
| Description | Microscopy Workshop Izmir, Turkey 4.2016 and 12.2016 |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Postgraduate students |
| Results and Impact | Organization of microscopy course in Izmir, Turkey including hands-on sessions |
| Year(s) Of Engagement Activity | 2016 |
| Description | Microscopy Workshop Lubljana, 9.2017 |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Undergraduate students |
| Results and Impact | Teaching of basics of optical microscopy to public/students |
| Year(s) Of Engagement Activity | 2017 |
| Description | Microscopy in school |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Schools |
| Results and Impact | Teaching of microscopy in regional schools |
| Year(s) Of Engagement Activity | 2015,2016 |
| Description | Oxford Bioimaging Festival |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Undergraduate students |
| Results and Impact | Co-organizer Oxford Bioimaging festival Large increase in enquiries to our group - many people wanting to know if they could obtain copies of the images we had for display in public areas. |
| Year(s) Of Engagement Activity | 2012,2013,2014,2015,2016 |
| URL | http://www.imaging.ox.ac.uk/activities/annual-festival/imagefest12 |
| Description | Oxford curiosity carnival |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Public/other audiences |
| Results and Impact | Presenting general activities of the institute/University to the public at an open event |
| Year(s) Of Engagement Activity | 2017 |
| Description | Webinar on STED Far-field Nanoscopy |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Media (as a channel to the public) |
| Results and Impact | At the request of LEICA, I put together a webinar (online presentation) to discuss my current work. This was advertised to their employees, and made publicly available Closer relationship with LEICA. They have since held a Symposium here at the WIMM to discuss Nanoscopy |
| Year(s) Of Engagement Activity | 2013,2014,2015 |
| URL | http://view6.workcast.net/?pak=8993948753416174 |