Newton001 SLEEP DEPRIVATION IN PARKINSON'S DISEASE: BRAZIL AND UNITED KINGDOM RESEARCH NETWORK
Lead Research Organisation:
University of Surrey
Department Name: Biochemistry & Physiology
Abstract
The project aims to address the important topic of sleep disturbances in Parkinson's disease (PD) which is a growing health issue in Brazil, since life expectancy is significantly increasing across the decades (1970: 58.9 years; 1980: 62.8 years; 1990: 66.6 years; 2011: 74.3 years - World Health Rankings) and PD affects 1-3% of people over 65 years. Prevalence of neurodegenerative diseases is increasing in developing countries, in Brazil this is estimated to be 5.3%. In addition, economic development is associated with increased urbanization and shift work, which negatively impacts on sleep quality, generating several health problems (e.g. obesity, higher incidence of cancer, cardiovascular and metabolic disturbances) and increasing demand on the public health system. This project thus aims to address a strategic health problem in Brazil and will create a scientific Brazil-UK network to study the basic mechanisms underlying PD with novel, state-of-the-art analytical tools and approaches.
The overall aim of the project is to investigate the behavioural, neurochemical and metabolic changes following sleep restriction in an animal model of PD. We hypothesise that sleep deprivation will produce cognitive deficits, neurochemical and metabolic disruption. The behavioural and neurochemical experiments will be undertaken in the Neurophysiology Laboratory (Profs Lima and Louzada, Federal University of Paraná), a centre of excellence for the study of Parkinson's disease in Brazil. Samples will be analysed by targeted liquid chromatography/mass spectrometry (LC/MS) metabolomics (Prof Skene (University of Surrey). Additional added value will be provided by employing a second metabolomics platform, untargeted 1H NMR metabolomics to characterise brain, plasma and urine metabolite changes in the rat model (Dr Swann, University of Reading). The application of two complementary analytical approaches ensures wide metabolome coverage and using multiple sample types allows the metabolic perturbations induced by sleep deprivation in PD to be studied at different levels.
The planned combination of behavioural, neurochemical and metabolomic approaches will enable a multi-faceted observation of circulating and central neuronal parameters that could identify possible molecular biomarkers of early neurodegeneration in PD with potential diagnostic value. There will also be a mutual exchange of researchers and postgraduate students between the UK and Brazil to share new ideas, techniques and approaches allowing mutual (global) growth. The results of the proposed experiments will advance understanding of the mechanisms of PD and its inter-relationship with sleep/wake processing. Creation of a Brazil-United Kingdom research network will also provide a foundation for future studies and collaboration.
The overall aim of the project is to investigate the behavioural, neurochemical and metabolic changes following sleep restriction in an animal model of PD. We hypothesise that sleep deprivation will produce cognitive deficits, neurochemical and metabolic disruption. The behavioural and neurochemical experiments will be undertaken in the Neurophysiology Laboratory (Profs Lima and Louzada, Federal University of Paraná), a centre of excellence for the study of Parkinson's disease in Brazil. Samples will be analysed by targeted liquid chromatography/mass spectrometry (LC/MS) metabolomics (Prof Skene (University of Surrey). Additional added value will be provided by employing a second metabolomics platform, untargeted 1H NMR metabolomics to characterise brain, plasma and urine metabolite changes in the rat model (Dr Swann, University of Reading). The application of two complementary analytical approaches ensures wide metabolome coverage and using multiple sample types allows the metabolic perturbations induced by sleep deprivation in PD to be studied at different levels.
The planned combination of behavioural, neurochemical and metabolomic approaches will enable a multi-faceted observation of circulating and central neuronal parameters that could identify possible molecular biomarkers of early neurodegeneration in PD with potential diagnostic value. There will also be a mutual exchange of researchers and postgraduate students between the UK and Brazil to share new ideas, techniques and approaches allowing mutual (global) growth. The results of the proposed experiments will advance understanding of the mechanisms of PD and its inter-relationship with sleep/wake processing. Creation of a Brazil-United Kingdom research network will also provide a foundation for future studies and collaboration.
Technical Summary
Parkinson's disease (PD) is the second most common neurodegenerative disease, afflicting about 1% of people over 65 years and 4-5% of people over 85 years. The major clinical features include asymmetric onset of bradykinesia, rigidity, rest tremor and disturbances in balance. PD patients also experience prodromal, non-motor features, such as sleep disorders (insomnia, restless legs and periodic limb movements, rapid eye movement (REM) sleep behaviour disorder, excessive daytime sleepiness) and neuropsychiatric symptoms (depression, anxiety, apathy). Sleep disturbances and metabolic alterations may thus precede the cardinal motor features of PD by many years, and have been considered as early biomarkers of the premotor phase of PD. Accordingly, the overall aim of the present project is to investigate the behavioural, neurochemical and metabolic changes following chronic partial sleep deprivation in an animal model of PD. We hypothesise that sleep deprivation will produce cognitive deficits, neurochemical and metabolic disruption.
Control and PD rats will be sleep deprived by "gentle stimulation" made by tapping or gentle shaking of the cage whenever drowsiness or attempts to engage in a sleeping posture are observed (from 7:00 to 13:00 h daily, totally 6 h of sleep deprivation per day during 21 days). The animals will be kept undisturbed from 13:00-07:00 h, totally 18 h/day sleep recovery. Hence, this protocol is consistent with a chronic sleep restriction procedure that is similar to the sleep restriction experienced in our society today. Behavioural testing will be conducted in the open-field and object recognition tests for analysis of motor and cognitive parameters. Blood, urine and brain samples will be collected and analysed by capillary electrophoresis with laser-induced detection (LIF) (neurochemical analysis) and targeted liquid chromatography/mass spectrometry (LC/MS) and NMR metabolomics analysis.
Control and PD rats will be sleep deprived by "gentle stimulation" made by tapping or gentle shaking of the cage whenever drowsiness or attempts to engage in a sleeping posture are observed (from 7:00 to 13:00 h daily, totally 6 h of sleep deprivation per day during 21 days). The animals will be kept undisturbed from 13:00-07:00 h, totally 18 h/day sleep recovery. Hence, this protocol is consistent with a chronic sleep restriction procedure that is similar to the sleep restriction experienced in our society today. Behavioural testing will be conducted in the open-field and object recognition tests for analysis of motor and cognitive parameters. Blood, urine and brain samples will be collected and analysed by capillary electrophoresis with laser-induced detection (LIF) (neurochemical analysis) and targeted liquid chromatography/mass spectrometry (LC/MS) and NMR metabolomics analysis.
Planned Impact
N/A
Organisations
- University of Surrey (Lead Research Organisation)
- Cochin Institute (Collaboration)
- Edmund Mach Foundation (Collaboration)
- Universidade de São Paulo (Collaboration)
- University of Milano-Bicocca (Collaboration)
- Karolinska Institute (Collaboration)
- Biomedical Research Institute of Lleida (Collaboration)
- University of Padova (Collaboration)
- IMPERIAL COLLEGE LONDON (Collaboration)
- University of Lleida (Collaboration)
- Wageningen University & Research (Collaboration)
- Optibiotix Health plc (Collaboration)
- University College Cork (Collaboration)
- National Institute for Public Health and Environment (RIVM) (Collaboration)
- University of Helsinki (Collaboration)
- Federal University of Rio Grande do Norte (Collaboration)
- Federal University of Paraná (Collaboration)
- Federal University of Paraná (Project Partner)
Publications
Aurich MF
(2017)
Olfactory impairment is related to REM sleep deprivation in rotenone model of Parkinson's disease.
in Sleep science (Sao Paulo, Brazil)
Bonomo R
(2020)
Metabolomics markers in Neurology: current knowledge and future perspectives for therapeutic targeting.
in Expert review of neurotherapeutics
Caspani G
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Microbe-Immune Crosstalk: Evidence That T Cells Influence the Development of the Brain Metabolome.
in International journal of molecular sciences
Caspani G
(2019)
Gut microbial metabolites in depression: understanding the biochemical mechanisms.
in Microbial cell (Graz, Austria)
Caspani G
(2019)
Small talk: microbial metabolites involved in the signaling from microbiota to brain
in Current Opinion in Pharmacology
Caspani G
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Metabolomic signatures associated with depression and predictors of antidepressant response in humans: A CAN-BIND-1 report.
in Communications biology
Caspani G
(2022)
Metabolic phenotyping of opioid and psychostimulant addiction: A novel approach for biomarker discovery and biochemical understanding of the disorder.
in British journal of pharmacology
Cavelli M
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Electrocortical high frequency activity and respiratory entrainment in 6-hydroxydopamine model of Parkinson's disease.
in Brain research
Cóppola-Segovia V
(2017)
ER Stress Induced by Tunicamycin Triggers a-Synuclein Oligomerization, Dopaminergic Neurons Death and Locomotor Impairment: a New Model of Parkinson's Disease.
in Molecular neurobiology
Dauvilliers Y
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Metabolomics Signature of Patients With Narcolepsy
in Neurology
De La Iglesia HO
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Ancestral sleep.
in Current biology : CB
Diaz Heijtz R
(2022)
Targeting microbial metabolites to treat autism.
in Nature medicine
Diaz Heijtz R
(2019)
Editorial overview: CNS diseases and the microbiome.
in Current opinion in pharmacology
Diessler S
(2018)
A systems genetics resource and analysis of sleep regulation in the mouse.
in PLoS biology
Dos Santos Lima G
(2020)
Disruption of neocortical synchronisation during slow-wave sleep in the rotenone model of Parkinson's disease
in Journal of Sleep Research
Fagotti J
(2019)
Chronic sleep restriction in the rotenone Parkinson's disease model in rats reveals peripheral early-phase biomarkers.
in Scientific reports
Giskeødegård GF
(2015)
Diurnal rhythms in the human urine metabolome during sleep and total sleep deprivation.
in Scientific reports
Honma A
(2020)
Effect of acute total sleep deprivation on plasma melatonin, cortisol and metabolite rhythms in females.
in The European journal of neuroscience
Hughes ME
(2017)
Guidelines for Genome-Scale Analysis of Biological Rhythms.
in Journal of biological rhythms
Ilkiw JL
(2019)
Perspectives for the association between olfactory disturbances and depression in Parkinson's disease.
in Neural regeneration research
Ilkiw JL
(2019)
Dopaminergic Lesion in the Olfactory Bulb Restores Olfaction and Induces Depressive-Like Behaviors in a 6-OHDA Model of Parkinson's Disease.
in Molecular neurobiology
Isherwood CM
(2017)
Twenty-four-hour rhythmicity of circulating metabolites: effect of body mass and type 2 diabetes.
in FASEB journal : official publication of the Federation of American Societies for Experimental Biology
Kmita LC
(2019)
Absence of a synergic nigral proapoptotic effect triggered by REM sleep deprivation in the rotenone model of Parkinson´s disease.
in Sleep science (Sao Paulo, Brazil)
Lees HJ
(2019)
Obesity and Cage Environment Modulate Metabolism in the Zucker Rat: A Multiple Biological Matrix Approach to Characterizing Metabolic Phenomena.
in Journal of proteome research
Letertre MPM
(2022)
Characterizing the metabolic effects of the selective inhibition of gut microbial ß-glucuronidases in mice.
in Scientific reports
Marini S
(2017)
Abnormalities in the Polysomnographic, Adenosine and Metabolic Response to Sleep Deprivation in an Animal Model of Hyperammonemia.
in Frontiers in physiology
Marqueze E
(2021)
Exogenous melatonin decreases circadian misalignment and body weight among early types
in Journal of Pineal Research
Marqueze EC
(2015)
Natural light exposure, sleep and depression among day workers and shiftworkers at arctic and equatorial latitudes.
in PloS one
Martins A
(2020)
The effect of urbanization on sleep, sleep/wake routine, and metabolic health of residents in the Amazon region of Brazil
in Chronobiology International
Martins AJ
(2016)
Effects of physical activity at work and life-style on sleep in workers from an Amazonian Extractivist Reserve.
in Sleep science (Sao Paulo, Brazil)
Moreno CR
(2015)
Sleep patterns in Amazon rubber tappers with and without electric light at home.
in Scientific reports
Moreno CRC
(2020)
Phenotypic plasticity of circadian entrainment under a range of light conditions.
in Neurobiology of sleep and circadian rhythms
Morton A
(2019)
Increased plasma melatonin in presymptomatic Huntington disease sheep ( Ovis aries ): Compensatory neuroprotection in a neurodegenerative disease?
in Journal of Pineal Research
Nehme PA
(2019)
Melatonin profiles during the third trimester of pregnancy and health status in the offspring among day and night workers: A case series.
in Neurobiology of sleep and circadian rhythms
Nehme PA
(2019)
Reduced melatonin synthesis in pregnant night workers: Metabolic implications for offspring.
in Medical hypotheses
Noseda ACD
(2021)
MT2 melatonin receptors expressed in the olfactory bulb modulate depressive-like behavior and olfaction in the 6-OHDA model of Parkinson's disease.
in European journal of pharmacology
Penny HA
(2022)
Rhythmicity of intestinal IgA responses confers oscillatory commensal microbiota mutualism.
in Science immunology
Prior K
(2021)
Synchrony between daily rhythms of malaria parasites and hosts is driven by an essential amino acid
in Wellcome Open Research
Prior KF
(2021)
Synchrony between daily rhythms of malaria parasites and hosts is driven by an essential amino acid.
in Wellcome open research
Rodrigues L
(2019)
Potential new therapies against a toxic relationship: neuroinflammation and Parkinson's disease
in Behavioural Pharmacology
Rodrigues L
(2020)
Genetics, Neurology, Behavior, and Diet in Parkinson's Disease
Rodrigues LS
(2019)
Olfaction in female Wistar rats is influenced by dopaminergic periglomerular neurons after nigral and bulbar lesions.
in Behavioural pharmacology
Salminen S
(2022)
Author Correction: The International Scientific Association of Probiotics and Prebiotics (ISAPP) consensus statement on the definition and scope of postbiotics.
in Nature reviews. Gastroenterology & hepatology
Salminen S
(2022)
Author Correction: Reply to: Postbiotics - when simplification fails to clarify
in Nature Reviews Gastroenterology & Hepatology
Salminen S
(2022)
Author Correction: Reply to: Postbiotics - when simplification fails to clarify.
in Nature reviews. Gastroenterology & hepatology
Santos P
(2016)
Cholinergic Oculomotor Nucleus Activity Is Induced by REM Sleep Deprivation Negatively Impacting on Cognition
in Molecular Neurobiology
| Description | Our findings showed that sleep restriction in an animal model of Parkinson's disease altered several behavioural (reversal of locomotor activity impairment; cognitive impairment; delay of rest-activity rhythm) and metabolic parameters (branched-chain amino acids, tryptophan pathway, phenylalanine, and lipoproteins, pointing to mitochondrial impairment). If combined, our results reveal a plethora of parameters that represents reliable early-phase biomarkers of Parkinson's disease which can easily be measured and could be translated to human studies. |
| Exploitation Route | Our findings in an animal model of Parkinson's disease revealed a panel of metabolite biomarkers that may signal early phase Parkinson's disease. These metabolites now need to be measured in human studies. |
| Sectors | Healthcare,Pharmaceuticals and Medical Biotechnology |
| Description | Our findings (metabolic signature in an animal model of Parkinson's disease combined with chronic sleep restriction) identified reliable early-phase PD biomarkers which can easily be measured and translated to human studies. Using metabolomics biomarkers to track Parkinson's disease progression in patients as well as monitor drug treatment responses will benefit Parkinson's patients and healthcare in general. |
| First Year Of Impact | 2019 |
| Sector | Education,Healthcare |
| Impact Types | Societal |
| Description | Genone scale analysis guidelines |
| Geographic Reach | Multiple continents/international |
| Policy Influence Type | Influenced training of practitioners or researchers |
| Impact | The published guidelines is a multi-author paper and will act as a blue print for large scale omics analysis of time-series data. |
| Description | Sleep and Circadian Biomarkers |
| Geographic Reach | Europe |
| Policy Influence Type | Membership of a guideline committee |
| Description | Talk at UNIFESP Brazil |
| Geographic Reach | South America |
| Policy Influence Type | Influenced training of practitioners or researchers |
| Impact | Deeper, improved understanding of the links between circadian timing, restricted sleep and metabolic dysfunction. |
| Description | Anticipation of meal time in humans |
| Amount | £810,047 (GBP) |
| Funding ID | BB/S01814X/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 09/2019 |
| End | 09/2022 |
| Description | FAPESP - CONFAP - UK Academies |
| Amount | R$ 35,000 (BRL) |
| Funding ID | 2016/50417-3 |
| Organisation | São Paulo Research Foundation (FAPESP) |
| Sector | Public |
| Country | Brazil |
| Start | 05/2017 |
| End | 04/2018 |
| Description | MRC Newton001 |
| Amount | £49,000 (GBP) |
| Funding ID | MR/N006321/1 |
| Organisation | Medical Research Council (MRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 03/2015 |
| End | 10/2016 |
| Description | Molecular mapping of SARS-CoV2 and the host response with multiomics mass spectrometry to stratify disease outcomes |
| Amount | £1,851,624 (GBP) |
| Funding ID | BB/V011456/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 10/2020 |
| End | 04/2022 |
| Description | Seasonal rhythms |
| Amount | $2,025,000 (USD) |
| Funding ID | NIH |
| Organisation | Columbia University |
| Sector | Academic/University |
| Country | United States |
| Start | 09/2016 |
| End | 08/2021 |
| Description | Technology Development Grant |
| Amount | £919,761 (GBP) |
| Funding ID | 223704/Z/21/Z |
| Organisation | Wellcome Trust |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 06/2022 |
| End | 05/2024 |
| Title | Metabolomics in an animal model of Parkinson's disease |
| Description | Combination of NMR and targeted LC-MS metabolomics approaches to provide broad coverage of the metabolome |
| Type Of Material | Physiological assessment or outcome measure |
| Year Produced | 2019 |
| Provided To Others? | Yes |
| Impact | Using both NMR and targeted metabolomics approaches to investigate the effect of chronic sleep restriction in an animal mode of Parkinson's Disease (PD) has identified a broader range of metabolites involved. We propose that these metabolites may serve as reliable early-phase PD biomarkers which can easily be measured and could be translated to human studies. |
| URL | https://www.ncbi.nlm.nih.gov/pubmed/30760786 |
| Title | Metabolomics in PD |
| Description | Database of metabolomics data from two analytical platforms (NMR and LC/MS) looking at the effect of chronic sleep restriction on an animal model of Parkinson's disease (PD) |
| Type Of Material | Database/Collection of data |
| Year Produced | 2017 |
| Provided To Others? | No |
| Impact | The research database will only be made available once these data are published. Once this has occurred the subsequent impact can be assessed. |
| Description | Bile acids |
| Organisation | Imperial College London |
| Department | Imperial College Trust |
| Country | United Kingdom |
| Sector | Charity/Non Profit |
| PI Contribution | Our expertise in chronobiology and sleep, access to biological samples, data and facilities |
| Collaborator Contribution | Access to their facilities and expertise in metabolomics and bile acid measurment |
| Impact | Research collaboration and research grant |
| Start Year | 2015 |
| Description | Collaboration with University College Cork |
| Organisation | University College Cork |
| Country | Ireland |
| Sector | Academic/University |
| PI Contribution | Due to the published work on the gut-brain axis and brain metabolomics a collaboration has developed with Professor John Cryan and Dr Gerard Clarke from University College Cork. My research group has analysed brain samples from rodent models generated in Cork to study the effects of Caesarean section on the blood-brain-barrier and the microbiota-gut-brain axis. |
| Collaborator Contribution | These partners provided the brain samples necessary to study the impact of C-section birth on the blood-brain-barrier and the microbiota-gut-brain axis. |
| Impact | This collaboration is multidisciplinary. My research group provides metabolomics expertise and the Cork teams provide expertise in neuroscience, neuropharmacology, and animal models. Due to the infancy of this collaborations there are no notable outputs to date. |
| Start Year | 2020 |
| Description | Fondazione Edmund Mach |
| Organisation | Edmund Mach Foundation |
| Country | Italy |
| Sector | Charity/Non Profit |
| PI Contribution | PhD studentship investigating probiotic strains that can improve sleep and anxiety-like behaviour via the production of gut microbial metabolites. PhD student is performing bacterial and mammalian metabolomics and in vitro culturing. |
| Collaborator Contribution | Part funded a PhD studentship. |
| Impact | Work its underway. Outputs in the form of peer-reviewed publications and commercial products are anticipated. |
| Start Year | 2021 |
| Description | Gut microbiota |
| Organisation | Imperial College London |
| Department | Department of Surgery and Cancer |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Metabolomics LC/MS dataset; expert knowledge of circadian rhythms |
| Collaborator Contribution | Expertise in gut microbiota analysis; training of research staff |
| Impact | Collaboration is multi-disciplinary (physiology/pharmacology; analytical chemistry). |
| Start Year | 2018 |
| Description | Marianne Schultzberg - Karolinska Insitutet, Sweden |
| Organisation | Karolinska Institute |
| Country | Sweden |
| Sector | Academic/University |
| PI Contribution | Following our publications applying metabolomics to study brain metabolism we were contacted by Prof Schultzberg to investigate brain alterations related to Alzheimer's disease , their modulation by a novel therapeutic, and the potential role of the Gut Microbiota in such outcomes. |
| Collaborator Contribution | Prof Schultzberg's research team at the Karolinska Institutet have developed an animal model of Alzheimer's disease and a novel therapeutic for inhibiting the deficits characteristic of the disease. They provide samples and expertise in this area. |
| Impact | Data is still being collected to produce a manuscript and serve as preliminary data for an international grant application. |
| Start Year | 2020 |
| Description | Melatonin nanocapsules |
| Organisation | Cochin Institute |
| Country | France |
| Sector | Academic/University |
| PI Contribution | Data analysis and joint papers |
| Collaborator Contribution | Data analysis and joint papers |
| Impact | Joint papers have been published |
| Start Year | 2020 |
| Description | Melatonin nanocapsules |
| Organisation | Universidade de São Paulo |
| Country | Brazil |
| Sector | Academic/University |
| PI Contribution | Data analysis and joint papers |
| Collaborator Contribution | Data analysis and joint papers |
| Impact | Joint papers have been published |
| Start Year | 2020 |
| Description | Model of Hyperammonemia |
| Organisation | University of Helsinki |
| Country | Finland |
| Sector | Academic/University |
| PI Contribution | Development of targeted LC/MS method to measure metabolites in rat brain tissue |
| Collaborator Contribution | Designed and performed sleep deprivation experiments in a rat model of hyperammonemia; collected brain tissue samples |
| Impact | Research publication and conference presentations |
| Start Year | 2016 |
| Description | Model of Hyperammonemia |
| Organisation | University of Padova |
| Country | Italy |
| Sector | Academic/University |
| PI Contribution | Development of targeted LC/MS method to measure metabolites in rat brain tissue |
| Collaborator Contribution | Designed and performed sleep deprivation experiments in a rat model of hyperammonemia; collected brain tissue samples |
| Impact | Research publication and conference presentations |
| Start Year | 2016 |
| Description | NMR and LC/MS metabolomics in Parkinsons disease |
| Organisation | Federal University of Paraná |
| Country | Brazil |
| Sector | Academic/University |
| PI Contribution | Our expertise in chronobiology and sleep, protocol design, LC/MS metabolomics and data sharing |
| Collaborator Contribution | Imperial's expertise in NMR metabolomics; Federal University of Parana's expertise in animal model of Parkinson's disease; National Institute for Public Health and Environment (RIVM) expertise in statistical and data analysis |
| Impact | Metabolomics data generation; Conference presentations; Grants and scholarship proposals; Collaboration is multi-disciplinary |
| Start Year | 2015 |
| Description | NMR and LC/MS metabolomics in Parkinsons disease |
| Organisation | Imperial College London |
| Department | Department of Surgery and Cancer |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Our expertise in chronobiology and sleep, protocol design, LC/MS metabolomics and data sharing |
| Collaborator Contribution | Imperial's expertise in NMR metabolomics; Federal University of Parana's expertise in animal model of Parkinson's disease; National Institute for Public Health and Environment (RIVM) expertise in statistical and data analysis |
| Impact | Metabolomics data generation; Conference presentations; Grants and scholarship proposals; Collaboration is multi-disciplinary |
| Start Year | 2015 |
| Description | NMR and LC/MS metabolomics in Parkinsons disease |
| Organisation | National Institute for Public Health and Environment (RIVM) |
| Country | Netherlands |
| Sector | Academic/University |
| PI Contribution | Our expertise in chronobiology and sleep, protocol design, LC/MS metabolomics and data sharing |
| Collaborator Contribution | Imperial's expertise in NMR metabolomics; Federal University of Parana's expertise in animal model of Parkinson's disease; National Institute for Public Health and Environment (RIVM) expertise in statistical and data analysis |
| Impact | Metabolomics data generation; Conference presentations; Grants and scholarship proposals; Collaboration is multi-disciplinary |
| Start Year | 2015 |
| Description | Optibiotix Health Ltd |
| Organisation | Optibiotix Health plc |
| Country | United Kingdom |
| Sector | Private |
| PI Contribution | PhD student investigating probiotic strains that are able to produce metabolites that can improve sleep quality and have anxiolytic properties in humans. Perform bacterial and mammalian Metabolomics. |
| Collaborator Contribution | Part funded a PhD studentship and provided a range of candidate probiotic strains. |
| Impact | PhD student part funded. |
| Start Year | 2021 |
| Description | Peripheral neuropathy metabolomics |
| Organisation | University of Milano-Bicocca |
| Country | Italy |
| Sector | Academic/University |
| PI Contribution | We will perform targeted LC/MS metabolomics on samples collected by our partners |
| Collaborator Contribution | Our partners will perform experiments (effect of chemotherapy on peripheral neuropathy in an animal model) and collect samples for our analysis |
| Impact | Experiments are ongoing. |
| Start Year | 2018 |
| Description | Prof Clara Belzer at Wageningen University |
| Organisation | Wageningen University & Research |
| Country | Netherlands |
| Sector | Academic/University |
| PI Contribution | Following some exciting research findings about the potential role of gut microbial metabolites in the early-life development of autism spectrum disorders, a collaboration has been set up (mediated by Danone Nutricia) with Prof Clara Belzer at Wageningen. A PhD student (Patrick Schimmel) working in Prof Belzer's research team is spending 9 weeks (commencing March 2022) at Southampton performing bacterial metabolomics to confirm the findings from our human study. |
| Collaborator Contribution | The group at Wageningen has performed several culture experiments (mono- and co-cultures) and collected culture supernatant for metabolomics. |
| Impact | Placement of a PhD student. PhD student has trained other lab members in bacterial culture techniques. |
| Start Year | 2021 |
| Description | Sleep in animal model of Parkinson's |
| Organisation | Biomedical Research Institute of Lleida |
| Country | Spain |
| Sector | Public |
| PI Contribution | Data analysis, postgraduate student exchange, joint publications |
| Collaborator Contribution | Data analysis, postgraduate student exchange, joint publications |
| Impact | Joint publications |
| Start Year | 2019 |
| Description | Sleep in animal model of Parkinson's |
| Organisation | Federal University of Rio Grande do Norte |
| Country | Brazil |
| Sector | Academic/University |
| PI Contribution | Data analysis, postgraduate student exchange, joint publications |
| Collaborator Contribution | Data analysis, postgraduate student exchange, joint publications |
| Impact | Joint publications |
| Start Year | 2019 |
| Description | Sleep in rotenone model of Parkinson's disease |
| Organisation | Federal University of Paraná |
| Country | Brazil |
| Sector | Academic/University |
| PI Contribution | Offered initial support to establish a network of researchers |
| Collaborator Contribution | Lima at UFPR has created a Network of researchers to facilitate funding opportunities and collaborative research |
| Impact | Joint publications |
| Start Year | 2017 |
| Description | Sleep in rotenone model of Parkinson's disease |
| Organisation | Federal University of Rio Grande do Norte |
| Country | Brazil |
| Sector | Academic/University |
| PI Contribution | Offered initial support to establish a network of researchers |
| Collaborator Contribution | Lima at UFPR has created a Network of researchers to facilitate funding opportunities and collaborative research |
| Impact | Joint publications |
| Start Year | 2017 |
| Description | Sleep in rotenone model of Parkinson's disease |
| Organisation | University of Lleida |
| Country | Spain |
| Sector | Academic/University |
| PI Contribution | Offered initial support to establish a network of researchers |
| Collaborator Contribution | Lima at UFPR has created a Network of researchers to facilitate funding opportunities and collaborative research |
| Impact | Joint publications |
| Start Year | 2017 |
| Description | Delivered a Metabolomics workshop to the Federal University of Ceara, Brazil |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Postgraduate students |
| Results and Impact | A workshop on metabolomics was delivered to researchers and clinicians based at the Federal University of Ceara in Northeastern Brazil. This two day workshop covered analytical chemistry, multivariate statistics, and biological interpretation of results. All statistical tools used were open access and the research groups had access to necessary analytical chemistry platforms so this added value to their research. |
| Year(s) Of Engagement Activity | 2020 |
| Description | European Academy of Neurology (EAN) |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Introduced the concept of how biological rhythms may underpin neurological diseases |
| Year(s) Of Engagement Activity | 2021 |
| URL | https://www.ean.org/congress2021 |
| Description | International Biomarkers Workshop |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Representatives from international Sleep and Circadian Rhythm Societies attended a day workshop (Boston, US) to discuss standardisation of methods to uncover sleep and circadian biomarkers. A white paper has been proposed. |
| Year(s) Of Engagement Activity | 2017 |
| Description | Invited lecture at the International Society for Nutritional Psychiatry Research (ISNPR) |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | I delivered a lecture for a webinar on gut microbial-metabolites involved in the gut-brain axis for the International Society for Nutritional Psychiatry Research. This was attended by ~60 researchers and clinicians involved in nutritional psychiatry research. |
| Year(s) Of Engagement Activity | 2022 |
| URL | http://www.isnpr.org |
| Description | Lecture delivered at University College Cork, Ireland |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Postgraduate students |
| Results and Impact | Delivered a talk at the University College Cork in Ireland 2020. The lecture was largely attended by principal investigators, post-doctoral researchers and PhD students. The lecture focused on our current understanding of gut microbial metabolism and how metabolites arising from this activity can impact on host health and disease. Discussions afterwards led to the emergence of several new collaborations between my research group and investigators based in Cork. This spanned potential projects relating to the gut-brain axis, exercise, muscle metabolism, classical microbiology. |
| Year(s) Of Engagement Activity | 2020 |
| Description | Member of the Organising and Programme Committee for Mind, Mood and Microbes - the international conference on the microbiota-gut-brain axis |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Part of the organising (1/4) and programme (1/14) committee for 'Mind, Mood and Microbes' - the international conference on the microbiota-gut-brain axis. Responsible for deciding the scientific direction of the conference and selecting and inviting speakers. |
| Year(s) Of Engagement Activity | 2020,2021 |
| URL | https://www.mindmoodmicrobes.org |
| Description | Milano-Bicocca Workshop |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Professional Practitioners |
| Results and Impact | Gave a talk at Neurobiology Workshop (University of Milan-Bicocca) emphasising the value of Metabolomics as a tool for neuroscience research. Developed a research collaboration with Prof Guido Cavaletti. We are actively seeking research funding. |
| Year(s) Of Engagement Activity | 2017 |
| Description | Mood, Mind and Microbes annual meeting Amsterdam 2019 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Other audiences |
| Results and Impact | An invited talk at the Mood, Mind and Microbes annual meeting in Amsterdam. The focus of my talk was the role of the microbiota in the gut-brain axis including in disease states such as Parkinson's. The audience was made up of academic researchers, post-graduate students, industry representatives from food and pharmaceutical companies as well as writers/journalists. A promotional video was created from the conference and I gave an interview that is now publicly available about the gut-brain axis. |
| Year(s) Of Engagement Activity | 2019 |
| URL | https://www.mindmoodmicrobes.org |
| Description | Panel member for a Q&A event for Mind, Mood & Microbes - the International conference on the microbiota-gut-brain axis |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | One of 8 experts on the microbiota-gut-brain axis that sat on the Q&A panel discussing emerging mechanisms and translational challenges and innovations in this area. Over 1000 individuals registered for this event from 48 countries. |
| Year(s) Of Engagement Activity | 2021 |
| URL | https://www.mindmoodmicrobes.org/mmm-expert-qa |
| Description | Peripheral Nerve Society |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Gave talk about Metabolomics as a tool for neurological research. |
| Year(s) Of Engagement Activity | 2021 |
| URL | https://www.pnsociety.com/files/Meetings/2021%20Virtual%20Experience/2021_PNS_Annual_Meeting_Virtual... |
| Description | Pint of Science - Human Brain? Microbial Mind? |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Public/other audiences |
| Results and Impact | This was a national outreach activity (Pint of Science) intended to educate the general public about novel areas of research occurring in the UK. It was designed to be an informal 15 minute talk delivered in a pub with minimal scientific jargon. Several questions were asked after the talk about how to improve the health of the intestinal microbiota through the diet. |
| Year(s) Of Engagement Activity | 2019 |
| URL | https://pintofscience.co.uk/ |
| Description | Scientific Advisory Board for the RENEW project on the Gut-Bone Axis led by Prof Hanne Bertram (Aarhus University) |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | A member of the Scientific Advisory Board for the RENEW project on the role of calcium absorption on the Gut-Bone axis funded by Innovation Fund Denmark. Providing expertise on the application of metabolomics and the gut microbiota. |
| Year(s) Of Engagement Activity | 2020,2021 |
| URL | https://pure.au.dk/portal/en/projects/renew--valorisering-af-sidestroemme-fra-mejeriindustrien-til-f... |
| Description | Talk at University of Manchester Centre For Biological Timing |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | Around 80 UK researchers attended my online Zoom talk at the University of Manchester Centre For Biological Timing. |
| Year(s) Of Engagement Activity | 2020 |
| Description | Talk delivered at the Medica Labmed Forum 2020 virtual conference on the biochemical language of the microbiota-gut-brain axis |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | A talk titled 'The biochemical language of the microbiota-gut-brain axis' was delivered at the virtual event of the MEDICA LABMED FORUM 2020 as part of the 'Progress in microbiome and infection diagnostics' session. This was largely targeted towards a clinical audience |
| Year(s) Of Engagement Activity | 2020 |
| URL | https://www.medica-tradefair.com/en/Forums_Conferences/Forums/MEDICA_LABMED_FORUM/MEDICA_LABMED_FORU... |
| Description | Talk on the Gut-Brain-axis at the European Behavioural Pharmacology Society / Canadian Biomarker Integration Network in Depression (CAN-BIND) - Behaviour to Biomarkers virtual workshop |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | A talk was delivered at the European Behavioural Pharmacology Society (EBPS) / Canadian Biomarker Integration Network in Depression (CAN-BIND) - Behaviour to Biomarkers virtual workshop. This was a 20 minute talk on the application of metabolomics to study the metabolic components of the gut-brain axis. |
| Year(s) Of Engagement Activity | 2021 |
| URL | https://www.canbind.ca/canbindwebsitetest/ebps/ |
| Description | UNIFESP graduate course in Chronobiology |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Postgraduate students |
| Results and Impact | Introduce PG and UG students at Federal University of Paraná (UFPR), Curitiba, Brazil to Chronobiology and its links to sleep and metabolism and Chrono-nutrition. |
| Year(s) Of Engagement Activity | 2020 |