Phosphorylation-mediated regulation of the NF-kB subunit c-Rel
Lead Research Organisation:
University of Liverpool
Department Name: Institute of Integrative Biology
Abstract
Nuclear Factor kappa B (NF-kB) is a central regulator of the immune, stress and inflammatory responses, and additionally plays key roles in cell differentiation and survival. Aberrant regulation of NF-kB is also associated with many pathological conditions, including inflammatory diseases and cancer.
Mass spectrometric and cell biological techniques will be used to identify and characterise the functions of post-translational modifications (PTMs) of the c-Rel NF-kB subunit in B cells. Biological mass spectrometry will be used in PTM site identification, protein quantification and top-down proteomics. Biochemical/cell biology based approaches will be used to elucidate the biological role/transcription mediation of the c-Rel modification sites that are found. This includes cell culture, nucleofection-based transfection of B-cell lymphoma cell lines, Western blotting, q-PCR analysis of c-Rel target gene expression and analysis of cell viability.
As the project progresses and more information is acquired about c-Rel and its interactions, functional experiments using c-Rel mutant expression plasmids will be used in order to gain more insight into the roles of c-Rel.
Mass spectrometric and cell biological techniques will be used to identify and characterise the functions of post-translational modifications (PTMs) of the c-Rel NF-kB subunit in B cells. Biological mass spectrometry will be used in PTM site identification, protein quantification and top-down proteomics. Biochemical/cell biology based approaches will be used to elucidate the biological role/transcription mediation of the c-Rel modification sites that are found. This includes cell culture, nucleofection-based transfection of B-cell lymphoma cell lines, Western blotting, q-PCR analysis of c-Rel target gene expression and analysis of cell viability.
As the project progresses and more information is acquired about c-Rel and its interactions, functional experiments using c-Rel mutant expression plasmids will be used in order to gain more insight into the roles of c-Rel.
Organisations
People |
ORCID iD |
Claire Eyers (Primary Supervisor) | |
Vinay Mistry (Student) |
Studentship Projects
Project Reference | Relationship | Related To | Start | End | Student Name |
---|---|---|---|---|---|
BB/M011186/1 | 01/10/2015 | 31/03/2024 | |||
1644429 | Studentship | BB/M011186/1 | 01/10/2015 | 31/12/2016 | Vinay Mistry |