How do plant resistance proteins activate defence gene expression upon effector perception?

Lead Research Organisation: University of East Anglia
Department Name: Sainsbury Laboratory

Abstract

Plant disease resistance (R) proteins enable plants to detect specific pathogen effector molecules, and then activate defence, yet mechanisms of defence activation upon R protein activation (Effector-Triggered Immunity, or ETI) are poorly understood. Arabidopsis RPS4 and RRS1 resistance genes act together to detect two different bacterial effector proteins, AvrRps4 from Pseudomonas sp., and PopP2, an acetyltransferase, from Ralstonia sp.. RPS4 is a typical TIR-NB-LRR R protein, but the RRS1 TIR-NB-LRR R protein also carries a WRKY DNA binding domain, that acts as a "bait" that detects the presence and/or activity of AvrRps4 and PopP2 (Williams et al 2014; 10.1126/science.1247357 Sarris et al 2015; 10.1016/j.cell.2015.04.024).
Activation of defence by RPS4/RRS1 results in rapid induction of ~40 ETI genes, including genes for salicylic acid biosynthesis, other defence enzymes and transcription regulators (Sohn et al, 2014 10.1371/journal.pgen.1004655).
The goal of the studentship is to understand how RPS4/RRS1 activates these ~40 coregulated ETI genes in Arabidopsis upon effector perception. The student will test if upon activation, RPS4/RRS1 associates with and changes the chromatin state of genes that are induced during ETI defence. The project will define changes in chromatin at ETI-induced genes upon activation, and analyse transcription factors that associate with defence gene promoters.

Publications

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Studentship Projects

Project Reference Relationship Related To Start End Student Name
BB/M011216/1 01/10/2015 30/09/2023
1799891 Studentship BB/M011216/1 01/10/2016 30/09/2020 Pok Man Ngou
 
Description Award still in progress
Exploitation Route The research has improved fundamental understanding of the plant immune system.
Sectors Agriculture, Food and Drink,Environment

 
Description JSPS Pre/Postdoctoral Fellowship for Foreign Researchers (Short-Term Award)
Amount ¥800,000 (JPY)
Funding ID PE18729 
Organisation RIKEN 
Sector Private
Country Japan
Start 11/2018 
End 01/2019
 
Description Convening a workshop in the JIC Undergraduate summer school 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Undergraduate students
Results and Impact Presentation of the basics of plant pathology.
Practical preparation of agro infiltration, Pseudomonas infitration and dab/trypan blue staining.
Assisting student with practical, answering question and suggestions about future career.
Socializing with undergrad student.
Year(s) Of Engagement Activity 2017
URL https://www.jic.ac.uk/training-careers/summer-schools/international-undergraduate/
 
Description Convening a workshop in the TSL summer school 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Presentation of the basics of plant pathology.
Practical preparation: Pseudomonas infitration and dab/trypan blue staining.
Assisting student with practical, answering question and going through the logic behind the protocol.
Year(s) Of Engagement Activity 2017
URL http://www.tsl.ac.uk/news/summer-school-plant-microbe-interactions/