Investigating the regulation by O2 of the nif and fix genes in Rhizobia - AfS, ENWW

Lead Research Organisation: University of Oxford
Department Name: Interdisciplinary Bioscience DTP

Abstract

Nif and fix genes are responsible for diazotrophic biological nitrogen fixation (BNF), the ability of specialised bacteria to convert atmospheric N2 into N-containing compounds utilisable by plants. BNF can enable plants to be grown without the application of artificial N containing fertilisers, providing the potential to greatly enhance crop yields in areas without ready fertiliser access. A better understanding of the regulation of these genes will allow the manipulation and possible transfer of BNF to other organisms. Low O2 is a known regulator of some nif and fix genes but not all of them. This work aims to discover the other regulators involved and use a proteomics-based approach to understand the metabolic processes that these regulators control.

Research themes:
Sustainably enhancing agricultural production.
Global food security

Publications

10 25 50

Studentship Projects

Project Reference Relationship Related To Start End Student Name
BB/M011224/1 01/10/2015 30/09/2023
1810130 Studentship BB/M011224/1 01/10/2015 30/09/2019 Nick Crang
 
Description TEM imagining to help characterise PHB mutants 
Organisation James Hutton Institute
Country United Kingdom 
Sector Public 
PI Contribution We produced a mutant strains of azorhizobium caulinodans with novel nitrogen fixing phenotypes. These mutant strains were applied to the host legume Sesbania Rostra and nodules were sent to our partner.
Collaborator Contribution Our collaboration partner performed transmission electron microscopy nodules, allowing us to visualise clearly the phenotype of the bacteroids within
Impact We will be using the images obtained as part of a publication
Start Year 2017
 
Description Development of Poole Lab website (Rhizosphere.org) 
Form Of Engagement Activity Engagement focused website, blog or social media channel
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact The Rhizosphere website is used to describe work performed, profiles lab members, lists outreach activities and celebrates achievements. Also includes a list of Lab publications.
Year(s) Of Engagement Activity 2014,2015,2016,2017,2018
URL https://rhizosphere.org
 
Description Plant Sciences Masterclass 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact 30 Secondary school students between 14 and 17 years of age attended a lecture at the Oxford Museum of Natural History on the GM work on tomatoes by Professor Lee Sweetlove from the Oxford Department of Plant Sciences. Students were split into groups of around a dozen and discussed through the benefits of GM, how it differs to traditional agricultural breeding techniques and whether or not the results Professor Sweetlove had achieved could have been reached via conventional means.

I was a discussion facilitator for one of the groups, answering questions, asking leading questions and trying my best to draw them out into the discussion.
Year(s) Of Engagement Activity 2017
 
Description Posters at ICNF, Granada, Spain, Sept. 2017 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact International conference. 11 posters from Lab. 2 short presentations
Year(s) Of Engagement Activity 2017
URL https://rhizosphere.org/lab-news/
 
Description Twitter account Rhizosphere @PooleLabOxford 
Form Of Engagement Activity Engagement focused website, blog or social media channel
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact Twitter account to publicise the work of the Poole Lab
Year(s) Of Engagement Activity 2017,2018,2019
URL https://twitter.com