Use of transgenic zebrafish as a tool to study nanomaterial inflammogenicity

Lead Research Organisation: Heriot-Watt University
Department Name: Sch of Engineering and Physical Science

Abstract

This project will use zebrafish to assess the inflammatory responses induced by nanomaterials (NMs) in order to identify if zebrafish are a uesful (alternative) tool to investigate NM mediated inflammatory responses (e.g. neutrophil accumulation). A panel of NMs will be tested to identify whether the physico-chemical properties of NMs influence their pathogenicity. This will require that the physico-chemical properties of the NMs under investigation are characterised thoroughly (e.g. using AFM, TEM, SEM, ICP-MS). Where possible state of the art microscopy will be used to visualise and track immune cell movement in zebrafish models, and to quantify their accumulation. Of benefit, is that the ability of some of these NMs to induce pulmonary inflammation has already been tested in previous in vivo (rodent) studies. This will allow us to identify whether zebrafish are an appropriate alternative to rodent testing.

Publications

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Studentship Projects

Project Reference Relationship Related To Start End Student Name
EP/N509474/1 01/10/2016 30/09/2021
1813003 Studentship EP/N509474/1 01/10/2016 30/09/2019 Suzanne Gillies
 
Description Developing the transgenic zebrafish larvae as a model organism for nanotoxicology and investigating the use of alternative in vitro assays using human cells (primary neutrophils) and cell lines (HL-60 neutrophil-like cells)
Exploitation Route Too early to say
Sectors Chemicals,Healthcare,Pharmaceuticals and Medical Biotechnology

 
Description Poster presentation at 'The Nanosafety Forum for Young Scientists' (Malta, September 2018) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact A poster presentation which provided an overview of the research findings was given at The Nanosafety Forum for Young Scientists', (Malta)
Year(s) Of Engagement Activity 2018
 
Description Poster presentation at the symposium "Fish and amphibian embryos as alternative models in toxicology and teratology" (Paris, December 2018) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact A poster was presented which provided an overview of the research findings of the project so far
Year(s) Of Engagement Activity 2018