Imaging the mechanism of nanopore sensing

Lead Research Organisation: Imperial College London
Department Name: Dept of Chemistry

Abstract

"Recent advances in 'fourth-generation' genome sequencing methods have led to a dramatic reduction in the cost per base - driving a marked expansion in our understanding of the subtleties and variations in how genetic profiles affect disease. In particular, Nanopore sequencing has been highlighted as a technology with the potential to further disrupt this field. In nanopore sensing, the flow of ions through a nanoscopic protein or solid-state pore is disrupted by the presence of an analyte. If this analyte is DNA, blockades in the current corresponding to the sequence are detected.

In collaboration with Oxford Nanopore Technologies (ONT), we will combine optical single channel recording and single-molecule fluorescence imaging to understand and then optimise the mechanistic steps of nanopore - analyte interactions; from membrane binding, to capture and readout. It is expected that a successful outcome will facilitate the development of improved nanopore sequencing and help design nanopore sensors for protein-protein, and protein-DNA interactions.
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Publications

10 25 50

Studentship Projects

Project Reference Relationship Related To Start End Student Name
EP/R512540/1 01/10/2017 30/09/2021
1935842 Studentship EP/R512540/1 30/09/2017 29/09/2021 Christopher Parperis