Rewiring of a Host Transcriptome by a Viral RNA-Binding Protein
Lead Research Organisation:
University of Oxford
Department Name: Interdisciplinary Bioscience DTP
Abstract
The HIV-1 protein Rev is responsible for the nuclear export of intronic viral RNA and is essential for viral replication. To function, Rev must interact with host cell molecules, though it's interactome remains largely undiscovered. This project aims to elucidate this interactome in the context of physiological infection by using HIV-1 replicons expressing tagged Rev. Rev will be immunoprecipitated by its tags and analysed by mass spectronomy analysis and RNA sequencing.
The pathophysiological consequences of these interactions will be investigated by monitoring viral fitness when Rev-interacting proteins and RNAs are overexpressed or knocked down.
Relevant BBSRC research areas
Data driven biology, systems approaches to the biosciences
The pathophysiological consequences of these interactions will be investigated by monitoring viral fitness when Rev-interacting proteins and RNAs are overexpressed or knocked down.
Relevant BBSRC research areas
Data driven biology, systems approaches to the biosciences
Organisations
Studentship Projects
| Project Reference | Relationship | Related To | Start | End | Student Name |
|---|---|---|---|---|---|
| BB/M011224/1 | 01/10/2015 | 31/03/2024 | |||
| 1946451 | Studentship | BB/M011224/1 | 01/10/2017 | 30/09/2021 |
| Description | We have uncovered a database of human proteins that interact with a crucial HIV protein called Rev, in near-physiological infection conditions. These proteins may represent novel theraputic targets for combating HIV. |
| Exploitation Route | Others may use our dataset to further probe the interactions of Rev and human proteins or use these proteins as theraputic targets. |
| Sectors | Pharmaceuticals and Medical Biotechnology |
| Description | Natural History Museum engagement sessions |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | For 2 hours on Saturday mornings between January and March 2020, I helped lead hands-on sessions in the Oxford Museum of Natural History for ~25 14-16 year old students from local schools. We learned molecular and geographical evolution principally. |
| Year(s) Of Engagement Activity | 2020 |