Mitochondrial and synaptic dysfunction in neurons from patients with OPA1- associated parkinsonism

Lead Research Organisation: University College London
Department Name: Institute of Neurology

Abstract

The project focuses on identifying and understanding novel mechanisms regulating mitochondrial quality control pathways, which are affected in a variety of neurodegenerative diseases, including Parkinson's Disease and Alzheimer's Disease. In the first part of the project, a high content mitophagy screen will be developed to identify these novel pathways in human neuroblastoma cells. All hits will then be validated and any discovery will be translated in iPSC-derived neurons from Parkinson's Disease patients, to identify any relevant potential therapeutic targets.

Publications

10 25 50
 
Title Mitophagy screen 
Description - high content images from mitophagy screen assay development, phosphatome and kinome libraries and GWAS library 
Type Of Material Database/Collection of data 
Year Produced 2018 
Provided To Others? No  
Impact -identification of novel hits that may be involved in mitophagy 
 
Description Understanding Parkinson's Disease GWAS hits 
Organisation Alzheimer's Research UK
Department UCL Drug Discovery Institute
Country United Kingdom 
Sector Charity/Non Profit 
PI Contribution - developing a high content screen for mitophagy - screening the GWAS candidates genes - investigating the biological pathways involved
Collaborator Contribution - investigating the genetic variants identified in the screen
Impact - selected candidate GWAS hits to screen (genetics, bioinformatics) - developed a robust high content siRNA screen for mitophagy (screening) - identified novel genes that might modulate mitophagy (biology)
Start Year 2017
 
Description Understanding Parkinson's Disease GWAS hits 
Organisation Medical Research Council (MRC)
Department MRC Mitochondrial Biology Unit
Country United Kingdom 
Sector Public 
PI Contribution - developing a high content screen for mitophagy - screening the GWAS candidates genes - investigating the biological pathways involved
Collaborator Contribution - investigating the genetic variants identified in the screen
Impact - selected candidate GWAS hits to screen (genetics, bioinformatics) - developed a robust high content siRNA screen for mitophagy (screening) - identified novel genes that might modulate mitophagy (biology)
Start Year 2017
 
Description Understanding Parkinson's Disease GWAS hits 
Organisation National Institute on Aging
Department Laboratory of Neurogenetics
Country United States 
Sector Public 
PI Contribution - developing a high content screen for mitophagy - screening the GWAS candidates genes - investigating the biological pathways involved
Collaborator Contribution - investigating the genetic variants identified in the screen
Impact - selected candidate GWAS hits to screen (genetics, bioinformatics) - developed a robust high content siRNA screen for mitophagy (screening) - identified novel genes that might modulate mitophagy (biology)
Start Year 2017
 
Description Understanding Parkinson's Disease GWAS hits 
Organisation University of Reading
Department School of Pharmacy Reading
Country United Kingdom 
Sector Academic/University 
PI Contribution - developing a high content screen for mitophagy - screening the GWAS candidates genes - investigating the biological pathways involved
Collaborator Contribution - investigating the genetic variants identified in the screen
Impact - selected candidate GWAS hits to screen (genetics, bioinformatics) - developed a robust high content siRNA screen for mitophagy (screening) - identified novel genes that might modulate mitophagy (biology)
Start Year 2017
 
Description Social Media Public Outreach 
Form Of Engagement Activity Engagement focused website, blog or social media channel
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact We have created Instagram and Twitter channels to show our research and science life to colleagues and the general public, to improve our science communication and network.
Year(s) Of Engagement Activity 2018,2019