Investigating the influence of various factors on the membrane insertion of DNA-based nanostructures.

Lead Research Organisation: University of Cambridge
Department Name: Physics

Abstract

Transmembrane proteins are of paramount importance in optimal functioning of cells owing to their function in regulation of membrane transport and therefore are relevant in the eld of medicine. DNA nanostructures can be used to mimic transmembrane proteins, however the knowledge of DNA-lipid interactions is still minimal in that no quantitative studies on DNA membrane insertion e ciency have been performed. The proposed project has been designed to solve this problem by using a novel microfluidic assay to quantify DNA insertion rates in lipid membranes, as well as observe the influence of both chemical and physical factors on the insertion rates. This quanti cation will then lead to design of novel DNA nanostructures with optimal transmembrane insertion e ciency. The envisioned assay will be generally applicable and specifcally used to explore insertion kinetics and transport properties of naturally occuring transmembrane proteins with the aid of a fluorescence readout.

Publications

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Studentship Projects

Project Reference Relationship Related To Start End Student Name
EP/R512461/1 01/10/2017 30/09/2021
1948668 Studentship EP/R512461/1 01/10/2017 30/09/2021 Akhila Kadgathur Jayaram