Exploring tankyrase regulation and cellular roles by CRISPR-based phenotpyic screens and functional studies
Lead Research Organisation:
Institute of Cancer Research
Department Name: Division of Structural Biology
Abstract
Establish a phenotypic screening system for tankyrase by CRISPR-X
Perform a CRISPR-X mutagenesis screen for tankyrase to identify novel regulatory principles in the context of Wnt-catenin signalling
Explore identified regulatory mechanisms in cell-based assays and biochemical studies using purified proteins
Perform a genome-wide CRISPR-Cas9 screen to identify homeostatic mechanisms that allow cells to tolerate the loss of tankyrase function
Perform a CRISPR-X mutagenesis screen for tankyrase to identify novel regulatory principles in the context of Wnt-catenin signalling
Explore identified regulatory mechanisms in cell-based assays and biochemical studies using purified proteins
Perform a genome-wide CRISPR-Cas9 screen to identify homeostatic mechanisms that allow cells to tolerate the loss of tankyrase function
People |
ORCID iD |
| Sebastian Guettler (Primary Supervisor) | |
| Sam Hibbs (Student) |
Studentship Projects
| Project Reference | Relationship | Related To | Start | End | Student Name |
|---|---|---|---|---|---|
| MR/R01583X/1 | 01/10/2018 | 30/09/2025 | |||
| 2119015 | Studentship | MR/R01583X/1 | 01/10/2018 | 31/12/2019 | Sam Hibbs |