The CHELL : A Bottom-Up approach to in vitro and in silico Minimal Life-like Constructs
Lead Research Organisation:
University of Oxford
Department Name: Oxford Chemistry
Abstract
Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.
Organisations
Publications
Kreizman R
(2009)
Core-shell PbI2@WS2 inorganic nanotubes from capillary wetting.
in Angewandte Chemie (International ed. in English)
Cocinero EJ
(2009)
Hydration of sugars in the gas phase: regioselectivity and conformational choice in N-acetyl glucosamine and glucose.
in Chemistry (Weinheim an der Bergstrasse, Germany)
Russell D
(2009)
Site-selective chemical protein glycosylation protects from autolysis and proteolytic degradation.
in Carbohydrate research
Doores K. J.
(2009)
A 'non-self' mimic of the natural epitope of anti-HIV antibody 2G12 shows enhanced antigenicity
in RETROVIROLOGY
Davis Benjamin G.
(2009)
Sugar and proteins: Strategies in synthetic biology
in ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
Simons J
(2009)
Conformational change and selectivity in explicitly hydrated carbohydrates
in Tetrahedron: Asymmetry
Allman SA
(2009)
Potent fluoro-oligosaccharide probes of adhesion in Toxoplasmosis.
in Chembiochem : a European journal of chemical biology
Floyd Nicola
(2009)
ORGN 260-An unexpected photo-induced site-selective protein cleavage reaction
in ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
Serres S
(2009)
Systemic inflammatory response reactivates immune-mediated lesions in rat brain.
in The Journal of neuroscience : the official journal of the Society for Neuroscience
Floyd N
(2009)
Thiyl glycosylation of olefinic proteins: S-linked glycoconjugate synthesis.
in Angewandte Chemie (International ed. in English)
Lin YA
(2009)
Olefin metathesis for site-selective protein modification.
in Chembiochem : a European journal of chemical biology
Boutureira O
(2009)
Accessible sugars as asymmetric olefin epoxidation organocatalysts: glucosaminide ketones in the synthesis of terminal epoxides.
in Organic & biomolecular chemistry
Harris LG
(2009)
Rewritable glycochips.
in Journal of the American Chemical Society
Chalker JM
(2009)
A convenient catalyst for aqueous and protein Suzuki-Miyaura cross-coupling.
in Journal of the American Chemical Society
Parry Alison L.
(2009)
Glycopolymer-functionalized gold nanoparticles: A new strategy toward synthetic anticancer vaccines
in ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
Chalker JM
(2009)
Chemical modification of proteins at cysteine: opportunities in chemistry and biology.
in Chemistry, an Asian journal
Cocinero E
(2009)
The Building Blocks of Cellulose: The Intrinsic Conformational Structures of Cellobiose, Its Epimer, Lactose, and Their Singly Hydrated Complexes
in Journal of the American Chemical Society
Floyd N
(2009)
Photoinduced, family-specific, site-selective cleavage of TIM-barrel proteins.
in Journal of the American Chemical Society
Fulton Z.
(2009)
Molecular mechanisms for enhancing the antigenicity of the carbohydrate epitope of the broadly neutralizing anti-HIV-1 antibody 2G12
in RETROVIROLOGY
Garnett JA
(2009)
Detailed insights from microarray and crystallographic studies into carbohydrate recognition by microneme protein 1 (MIC1) of Toxoplasma gondii.
in Protein science : a publication of the Protein Society
Cocinero EJ
(2009)
Peptide secondary structures in the gas phase: consensus motif of N-linked glycoproteins.
in Journal of the American Chemical Society
Chalker JM
(2009)
Enabling olefin metathesis on proteins: chemical methods for installation of S-allyl cysteine.
in Chemical communications (Cambridge, England)
Davis BG
(2009)
The linear assembly of a pure glycoenzyme.
in Angewandte Chemie (International ed. in English)
Seebach D
(2009)
Polymer Backbone Conformation-A Challenging Task for Database Information Retrieval
in Angewandte Chemie International Edition
PĂ©rez-Victoria I
(2009)
Saturation transfer difference NMR reveals functionally essential kinetic differences for a sugar-binding repressor protein.
in Chemical communications (Cambridge, England)
Gardner PM
(2009)
Sugar synthesis in a protocellular model leads to a cell signalling response in bacteria.
in Nature chemistry
Davis B
(2009)
A silver-lined anniversary of Fleet iminosugars: 1984-2009, from DIM to DRAM to LABNAc
in Tetrahedron: Asymmetry
Van Kasteren SI
(2009)
Glyconanoparticles allow pre-symptomatic in vivo imaging of brain disease.
in Proceedings of the National Academy of Sciences of the United States of America
Davis B
(2009)
Sugars and proteins: New strategies in synthetic biology
in Pure and Applied Chemistry
Dickens A. M.
(2009)
PLS models discriminate types of MS from spectroscopic analysis of plasma and urinele
in MULTIPLE SCLEROSIS
Hong Sung You
(2009)
Functionalization of single walled carbon nanotubes with carbohydrates
in ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
Gamblin DP
(2009)
Glycoprotein synthesis: an update.
in Chemical reviews
French AC
(2009)
High-purity discrete PEG-oligomer crystals allow structural insight.
in Angewandte Chemie (International ed. in English)
Lin YA
(2010)
Olefin cross-metathesis on proteins: investigation of allylic chalcogen effects and guiding principles in metathesis partner selection.
in Journal of the American Chemical Society
Kemper S
(2010)
Group epitope mapping considering relaxation of the ligand (GEM-CRL): including longitudinal relaxation rates in the analysis of saturation transfer difference (STD) experiments.
in Journal of magnetic resonance (San Diego, Calif. : 1997)
Scanlan EM
(2010)
Synthesis and solution-phase conformation of the RG-I fragment of the plant polysaccharide pectin reveals a modification-modulated assembly mechanism.
in Journal of the American Chemical Society
Patel MK
(2010)
Flow chemistry kinetic studies reveal reaction conditions for ready access to unsymmetrical trehalose analogues.
in Organic & biomolecular chemistry
Hong SY
(2010)
Filled and glycosylated carbon nanotubes for in vivo radioemitter localization and imaging.
in Nature materials
Wyszynski FJ
(2010)
Inverted regioselectivity of C-H amination: Unexpected oxidation at beta- rather than gamma-C-H.
in Organic & biomolecular chemistry
Wyszynski F
(2010)
Dissecting tunicamycin biosynthesis by genome mining: cloning and heterologous expression of a minimal gene cluster
in Chemical Science
Fernández-González M
(2010)
Site-selective chemoenzymatic construction of synthetic glycoproteins using endoglycosidases
in Chemical Science
Davis BG
(2010)
Enzyme catalysis: sweet flexibility.
in Nature chemistry
Boutureira O
(2010)
Fluoroglycoproteins: ready chemical site-selective incorporation of fluorosugars into proteins.
in Chemical communications (Cambridge, England)
Hong S
(2010)
Synthesis and characterization of WS2 inorganic nanotubes with encapsulated/intercalated CsI
in Nano Research
Pasparakis G
(2010)
Controlled polymer synthesis--from biomimicry towards synthetic biology.
in Chemical Society reviews
Garnier P
(2010)
Lectin-directed enzyme activated prodrug therapy (LEAPT): Synthesis and evaluation of rhamnose-capped prodrugs.
in Journal of drug targeting
Patel MK
(2010)
Analysis of the dispersity in carbohydrate loading of synthetic glycoproteins using MALDI-TOF mass spectrometry.
in Chemical communications (Cambridge, England)
Lin YA
(2010)
The allylic chalcogen effect in olefin metathesis.
in Beilstein journal of organic chemistry
Doores KJ
(2010)
A nonself sugar mimic of the HIV glycan shield shows enhanced antigenicity.
in Proceedings of the National Academy of Sciences of the United States of America
| Description | We found that we were not only able to make an artificial chemical cell (something we called the 'chell') but that we could also create sufficient information from its metabolism for it to communicate with living (bacterial cells). This 'conversation' between artificial cells and living cells highlights that our notions of cellularity (and life) can be examined through fundamental experiments. |
| Exploitation Route | These ideas have provoked various communities that examine cells right down to those who are interested in the emergence of complexity and even studying the origins of life. It provoked an analysis of the use of 'chemical cells' as potential 'living drugs', for example - see https://vimeo.com/10274649. |
| Sectors | Education Pharmaceuticals and Medical Biotechnology |
| URL | http://users.ox.ac.uk/~dplb0149/index.html |
| Description | BGD and group members have appeared on the radio, television, (BBD, Channel 5), newspapers (Times, New Scientist) science festivals around the world (Cheltenham, Kent, Edinburgh, Times Lit., Sydney) describing this work. We have given talks in schools to inspire the next generation. This work has been widely featured as one of the very first SynBio grants and that led to an expansion of the UK's SynBio community. It has also provoked a community of those more broadly interested in these concepts, including designers and artists - see https://vimeo.com/10274649 or http://www.daisyginsberg.com/work/synthesis-exchange-laboratory |
| First Year Of Impact | 2009 |
| Sector | Chemicals,Creative Economy,Education,Pharmaceuticals and Medical Biotechnology,Other |
| Impact Types | Cultural Societal Economic Policy & public services |
| Company Name | GlycoForm |
| Description | |
| Year Established | 2002 |
| Impact | Employed >20 people over 10 years and provided a model for how synthetic protein drugs might be constructed and used. The technology for this company has now been used by major US companies. |