i-Bacillus: Adapting Bacillus licheniformis for 21st century IB applications
Lead Participant:
INGENZA LIMITED
Abstract
Bacillus licheniformis is a preferred host for the production of industrial enzymes, including proteases
for detergent and amylases and cellulases for food and biofuels. To advance its genetics/utility our key
objective is to deploy SynBio tools to improve endogenous and heterologous enzyme production
economics in B. licheniformis for exploitation via leading end users. Genome delivery technologies will
amplify/target genes to locations validated for high-level/predictable expression, overcoming issues
associated with non-targeted integration. Nuclease-based genome editing will a) address yield-
compromising aspects of host metabolism under stress conditions identified through systems biology
and b) capitalize on in situ protein engineering to improve endogenous enzyme function including
thermotolerance and optimal activity under operating conditions. Success in these areas will reduce the
cost and improve the versatility and efficiency of industrial enzymes produced in B. licheniformis.
for detergent and amylases and cellulases for food and biofuels. To advance its genetics/utility our key
objective is to deploy SynBio tools to improve endogenous and heterologous enzyme production
economics in B. licheniformis for exploitation via leading end users. Genome delivery technologies will
amplify/target genes to locations validated for high-level/predictable expression, overcoming issues
associated with non-targeted integration. Nuclease-based genome editing will a) address yield-
compromising aspects of host metabolism under stress conditions identified through systems biology
and b) capitalize on in situ protein engineering to improve endogenous enzyme function including
thermotolerance and optimal activity under operating conditions. Success in these areas will reduce the
cost and improve the versatility and efficiency of industrial enzymes produced in B. licheniformis.
Lead Participant | Project Cost | Grant Offer |
---|---|---|
INGENZA LIMITED | £566,485 | £ 396,540 |
  | ||
Participant |
||
NEWCASTLE UNIVERSITY | ||
UNIVERSITY OF NEWCASTLE | £359,933 | |
INNOVATE UK |
People |
ORCID iD |