Engineering molecular membrane pores
Lead Research Organisation:
University of Nottingham
Department Name: School of Life Sciences
Abstract
Solid state NMR mini-project: The objective is to give in a nutshell a flavour of the continuity in biological research from protein expression and purification, sample preparation through biophysical analysis and characterisation. Week 1-2: Recombinant protein will be overexpressed, purified and characterised with and without NMR labels; Week 3-4: Model membranes with reconstituted protein will be prepared and converted into oriented bilayer fragments, bicelles. The lipid membranes will be studied by solid state NMR to obtain a 15N spectrum form the protein. Week 5-6: Spectral simulations will be carried out to simulate the protein orientation in bicelles and these will be compared to experimental spectra to assess protein orientation.
Organisations
People |
ORCID iD |
Boyan Bonev (Primary Supervisor) |
Studentship Projects
Project Reference | Relationship | Related To | Start | End | Student Name |
---|---|---|---|---|---|
BB/M008770/1 | 01/10/2015 | 31/03/2024 | |||
1803599 | Studentship | BB/M008770/1 | 01/10/2016 | 24/03/2021 |