Determination of laminin LN domain roles in tissue function, using Pierson syndrome and the lens capsule as a model.
Lead Research Organisation:
University of Liverpool
Department Name: Institute of Ageing and Chronic Disease
Abstract
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Organisations
People |
ORCID iD |
| Kevin Hamill (Primary Supervisor) | |
| Liam Shaw (Student) |
Publications
Shaw L
(2020)
CRISPR-Cas9-mediated labelling of the C-terminus of human laminin ß1 leads to secretion inhibition.
in BMC research notes
Studentship Projects
| Project Reference | Relationship | Related To | Start | End | Student Name |
|---|---|---|---|---|---|
| BB/M011186/1 | 30/09/2015 | 31/03/2024 | |||
| 1945182 | Studentship | BB/M011186/1 | 30/09/2017 | 31/12/2021 | Liam Shaw |
| NE/W503083/1 | 31/03/2021 | 30/03/2022 | |||
| 1945182 | Studentship | NE/W503083/1 | 30/09/2017 | 31/12/2021 | Liam Shaw |
| Description | The first outcome of our research was to implement 2 mutations to the basement membrane protein laminin 511 that prevent laminin network formation by blocking polymerisation. The first mutation was a mutation that mimicked Pierson syndrome, but we have not yet been able to successfully implement this mutation. The second, more successful mutation we have tried to implement is the MDC1A mimic to laminin 511. Initial characterization of this mutant has shown that mutations that prevent the laminin 511 from polymerising have a significant phenotype compared to wildtype cells, with reduced proliferation, adhesion and motility, as well as a reduction in surface levels of integrins responsible for laminin binding in the cell. We will next aim to take this into 3D cultures as well as establishing a Pierson syndrome mimic to further understand the role of laminin polymerisation |
| Exploitation Route | If we can understand the role of laminin polymerisation in cells, particular stem cells associated with a laminin 511 network, then we can use this knowledge to better maintain stem cell populations |
| Sectors | Manufacturing including Industrial Biotechology Pharmaceuticals and Medical Biotechnology |