Investigating the function and regulation of UbcH10-APC/C
Lead Research Organisation:
University of Birmingham
Department Name: Institute of Cancer and Genomic Sciences
Abstract
The Anaphase-Promoting Complex/Cyclosome (APC/C) is an E3 ubiquitin ligase that, through targeting protein substrates for polyubiquitylation and 26S proteasome-mediated degradation coordinates the progression of cells through mitosis and the successive G1 phase of the cell-cycle. APC/C E3 ligase activity is stimulated, in the presence of the E2-ubiquitin conjugating enzymes UbcH10 and Ube2S, by the temporally coordinated recruitment of one of two related activator proteins, Cdc20 or Cdh1, to the APC/C; Cdc20 and Cdh1 also serve in conjunction with particular APC/C subunits to bind substrates. Published work from our laboratory has previously determined that the ubiquitin ligase activity of the APC/C is regulated by the transcriptional co-activators CBP and p300. We have also determined that the DNA damage response, and scaffold protein, MDC1 regulates APC/C-Cdc20 activity during mitosis by promoting Cdc20 association with the APC/C, whilst the transcriptional repressor, TIF1y, also regulates APC/C-Cdc20 activity in mitosis. We have also established that the DDR protein, 53BP1, is both an inhibitor, and substrate of the APC/C, and regulates entry into, and progression through, mitosis. To further our understanding of APC/C function we have recently characterized the interactome of the APC/C E2-conjugating enzyme, UbcH10. The current project will investigate the ability of UbcH10-interacting proteins to modulate UbcH10-APC/C activity and function.
Organisations
People |
ORCID iD |
Andrew Turnell (Primary Supervisor) | |
Jessica Bula (Student) |
Studentship Projects
Project Reference | Relationship | Related To | Start | End | Student Name |
---|---|---|---|---|---|
BB/M01116X/1 | 01/10/2015 | 31/03/2024 | |||
2097737 | Studentship | BB/M01116X/1 | 01/10/2018 | 31/05/2024 | Jessica Bula |