High resolution phylogenetic analysis of livestock-associated Staphylococcus aureus.
Lead Research Organisation:
University of Edinburgh
Department Name: The Roslin Institute
Abstract
An understanding of the basis for the emergence of endemic diseases of livestock is critical for designing new approaches for their control and to prevent new pathogens from emerging. In particular, the role of human activities such as industrialization and globalization in the evolution of pathogens has not been well examined to date. Staphylococcus aureus is notorious as a major human pathogen and is also an important pathogen of farmed animals including cows, sheep, goats, poultry and pigs, resulting in huge economic losses. Recent studies have provided evidence that S. aureus strains affecting livestock have evolved from human strains and have undergone genetic adaptation to cause endemic diseases of animals. Furthermore, some livestock-associated strains have the capacity to cause zoonotic infections of humans. In the current project we will use high throughput sequencing technology to achieve a comprehensive genetic analysis of livestock-associated S. aureus. The project will reveal the evolutionary origin of livestock-associated S. aureus, how they have adapted to animal hosts, and their potential to cause human infections. The genetic analyses will also facilitate the identification of targets for developing new approaches to controlling endemic livestock disease. Overall, the project will contribute important new insights into a critical animal welfare, food security and public health issue.
Technical Summary
The emergence of pathogens causing endemic diseases of livestock is an important food security and public health issue. Staphylococcus aureus is an important human and animal pathogen and a major economic burden worldwide. Recent studies indicate that some animal-specific lineages have evolved from human progenitor strains followed by host-adaptive diversification. Importantly, human activities such as domestication and globalization may have contributed to the emergence of livestock-associated S. aureus by providing increased opportunities for cross-species host-jumps and promoting global dissemination. Furthermore, reports that some livestock-associated strains have the capacity to cause human zoonotic infection is a major public health concern. The project represents a high resolution genetic analysis of livestock-associated S. aureus to resolve the evolutionary history of all major livestock-associated S. aureus, including the molecular basis for host adaptation and the timing of predicted host-jumps. For clonal complexes associated with multiple animal and human hosts, this approach will allow us to identify intraclonal genetic variation, determine the geographic distribution of subtypes, and predict their zoonotic potential. Furthermore, the fundamental molecular processes contributing to adaptive genome diversification will be examined and genetic markers for host-specificity identified, leading to the identification of novel targets for the control of S. aureus livestock diseases. Overall, the project will contribute fundamental insights into an endemic pathogen of livestock which could be applied to improve animal health, and protect food security and public health
Planned Impact
It is anticipated that there will be a broad-ranging array of beneficiaries of the proposed project. In the commercial sector, companies involved in the breeding and distribution of broiler poultry and other livestock will benefit from an understanding of of the source of endemic pathogens and how they are disseminated. In a recent paper, we demonstrated that infections of broiler poultry were caused by resident S. aureus of human evolutionary origin which were being disseminated globally. We suggested that decolonization of birds should result in fewer infections. We expect that the findings relating to other livestock S. aureus resulting from the current study would inform on ways to reduce transmission of infections of those species which would be of interest to livestock breeders and farmers. Furthermore, policymakers involved in the regulation of livestock trade would benefit from an improved understanding of the role of industry in the dissemination of pathogens. Policymakers concerned with food safety including the UK Food Standards Agency and the European Food Safety Authority would benefit from our data relating to the prevalence of S. aureus strains on livestock and their potential to cause disease of humans. In addition, if livestock S. aureus is discovered to be a major potential source of new virulent strains affecting humans, the data could be used by bodies such as the UK Health Protection Agency which aims to provide integrated support to limit the dangers to human health from infections including S. aureus. In turn, the general public and particularly those most susceptible to S. aureus infection would benefit. We would anticipate that the research would directly impact on animal health by informing ways to prevent the spread of livestock pathogens and to prevent the emergence of new pathogens affecting livestock. In addition, although beyond the scope of the current proposal, the research could ultimately result in the design of novel therapeutics for controlling diseases such as mastitis of cows, sheep and goats, and skeletal infections of poultry. Accordingly, pharmaceutical companies developing products for animal health could directly benefit from the research. In turn this would generate wealth and contribute to the UK economy. The research will provide new information relating to the basis for the emergence of pathogens and thus could ultimately contribute to approaches to conserving global food security.
Organisations
- University of Edinburgh (Lead Research Organisation)
- Biomedicine Research Institute of Buenos Aires - CONICET - Partner Institute of the Max Planck Society (Collaboration)
- Mississippi State University (Collaboration)
- University of Helsinki (Collaboration)
- UNIVERSITY OF EDINBURGH (Collaboration)
- IMPERIAL COLLEGE LONDON (Collaboration)
Publications
Bacigalupe R
(2019)
A multihost bacterial pathogen overcomes continuous population bottlenecks to adapt to new host species.
in Science advances
Bonsaglia ECR
(2018)
Molecular epidemiology of methicillin-susceptible Staphylococcus aureus (MSSA) isolated from milk of cows with subclinical mastitis.
in Microbial pathogenesis
Chen J
(2018)
Genome hypermobility by lateral transduction.
in Science (New York, N.Y.)
Fitzgerald JR
(2016)
Genomics of Natural Populations of Staphylococcus aureus.
in Annual review of microbiology
Gibbons CL
(2016)
Not just a matter of size: a hospital-level risk factor analysis of MRSA bacteraemia in Scotland.
in BMC infectious diseases
Guinane C
(2014)
The role of horizontal gene transfer in Staphylococcus aureus host adaptation
in Virulence
Haag AF
(2019)
Staphylococcus aureus in Animals.
in Microbiology spectrum
Holden MT
(2013)
A genomic portrait of the emergence, evolution, and global spread of a methicillin-resistant Staphylococcus aureus pandemic.
in Genome research
Koop G
(2017)
Identification of LukPQ, a novel, equid-adapted leukocidin of Staphylococcus aureus.
in Scientific reports
Description | To date, we have produced the largest phylogeny of Staphylococcus aureus to date, made up of 800 isolates from humans and animals. We have quantified the frequency of host-switching events that have occurred during the evolutionary history of S. aureus. The key host-species are humans and cows that appear to be hubs for host-switching events. In particular, cows represent the major reservoir for the emergence of new pathogenic clones that originated in animals. Some clones have higher rates of host-transition than others implying the genetic background is important for host-switching potential. We have identified key roles for the accessory (non-shared) genome, and for mutations in the core genome in adaptation to different host species. Our data have revealed novel mobile genetic elements associated with host-switch events that provide new avenues for investigating the capacity of S. aureus to colonise and infect livestock. We are currently investigating the role of specific MGE in host-pathogen interactions. The genome sequence data has been used to identify conserved predicted antigens that are being used for vaccine development. We have now published a paper in the journal Nature Ecology and Evolution that describes these findings. |
Exploitation Route | Our work will provide an excellent framework for future studies of the evolution of S. aureus by providing phylogenetic context for the understanding of any strain of interest. We have provided broad new insights into the capacity of S. aureus to adapt to different species leading to the identification of potential new targets for therapeutic development. The large number of MGE identified in the current study provides numerous avenues for the investigation of mechanisms of host-adaptation and pathogenesis. |
Sectors | Agriculture, Food and Drink,Healthcare,Pharmaceuticals and Medical Biotechnology |
Description | BBSRC LINK |
Amount | £336,000 (GBP) |
Funding ID | BB/K00638X/1 |
Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
Sector | Public |
Country | United Kingdom |
Start | 08/2013 |
End | 09/2016 |
Description | One Health Models of Disease: Science, Ethics and Society |
Amount | £5,328,962 (GBP) |
Funding ID | 218471/Z/19/Z |
Organisation | Wellcome Trust |
Sector | Charity/Non Profit |
Country | United Kingdom |
Start | 10/2020 |
End | 09/2028 |
Description | The Dogs Trust |
Amount | £242,000 (GBP) |
Organisation | Dogs Trust |
Sector | Charity/Non Profit |
Country | United Kingdom |
Start | 06/2019 |
End | 05/2022 |
Description | Wellcome Trust Collaborative award |
Amount | £1,200,000 (GBP) |
Organisation | Wellcome Trust |
Sector | Charity/Non Profit |
Country | United Kingdom |
Start | 01/2017 |
End | 12/2021 |
Title | Bovine S. aureus sequence dataset and set of conserved vaccine antigens |
Description | The reverse vaccinology approach has resulted in a representative sequence dataset and list of conserved predicted antigens |
Type Of Material | Biological samples |
Year Produced | 2017 |
Provided To Others? | No |
Impact | Not yet publicly available but will be useful resource to research community |
Title | Bovine S. aureus sequence dataset |
Description | Whole genome sequence data for 1080 S. aureus isolates from bovine mastitis |
Type Of Material | Database/Collection of data |
Year Produced | 2021 |
Provided To Others? | No |
Impact | None yet |
Title | Emma Raftis PDRA Dataset |
Description | Some data produced during Emma Raftis postdoc looking at phylogenomics of S. aureus host adaptation |
Type Of Material | Database/Collection of data |
Year Produced | 2017 |
Provided To Others? | Yes |
Description | Collaboration with Daniel Sordelli |
Organisation | Biomedicine Research Institute of Buenos Aires - CONICET - Partner Institute of the Max Planck Society |
Country | Argentina |
Sector | Public |
PI Contribution | Genome sequence analysis |
Collaborator Contribution | Provision of bacterial isolates |
Impact | Paper published in Scientific reports and one in preparation |
Start Year | 2020 |
Description | Collaboration with Jose Penades |
Organisation | Imperial College London |
Country | United Kingdom |
Sector | Academic/University |
PI Contribution | Evolutionary genomic analysis of host-adaptation, lateral transduction |
Collaborator Contribution | Mechanistic analysis of host-adaptation, Lateral transduction |
Impact | Numerous publications |
Start Year | 2012 |
Description | Collaboration with Jukka Corander, University of Helsinki |
Organisation | University of Helsinki |
Country | Finland |
Sector | Academic/University |
PI Contribution | We led the study, provided sequence information and carried out the majority of the analysis |
Collaborator Contribution | Prof Corander and his team carried out computational analyses |
Impact | Paper: Spoor et al, Microbial Gen 2015 |
Start Year | 2013 |
Description | Collaboration with Keun Seok Seo |
Organisation | Mississippi State University |
Country | United States |
Sector | Academic/University |
PI Contribution | Collaborative research into the function of bacterial superantigens- sequence, evolutionary, and functional analysis |
Collaborator Contribution | functional analysis of bacterial superantigens |
Impact | 1: Moon BY, Park JY, Hwang SY, Robinson DA, Thomas JC, Fitzgerald JR, Park YH, Seo KS. Phage-mediated horizontal transfer of a Staphylococcus aureus virulence-associated genomic island. Sci Rep. 2015 Apr 20;5:9784. doi: 10.1038/srep09784. PubMed PMID: 25891795; PubMed Central PMCID: PMC4402969. 2: Wilson GJ, Seo KS, Cartwright RA, Connelley T, Chuang-Smith ON, Merriman JA, Guinane CM, Park JY, Bohach GA, Schlievert PM, Morrison WI, Fitzgerald JR. A novel core genome-encoded superantigen contributes to lethality of community-associated MRSA necrotizing pneumonia. PLoS Pathog. 2011 Oct;7(10):e1002271. doi: 10.1371/journal.ppat.1002271. PubMed PMID: 22022262; PubMed Central PMCID: PMC3192841. |
Start Year | 2010 |
Description | Collaboration with Mark Woolhouse |
Organisation | University of Edinburgh |
Department | British Heart Foundation Centre for Cardiovascular Science |
Country | United Kingdom |
Sector | Academic/University |
PI Contribution | Mark provided epidemiological expertise- we provided sequence-based analysis. |
Collaborator Contribution | None |
Impact | Publications |
Start Year | 2012 |
Description | Development of new public engagement activity |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Schools |
Results and Impact | Development of outreach activity on theme of bacterial host-adaptation funded by Wellcome Trust HostBusters! Highschool activity. Midlothian Science Festival 2019. Midlothian, UK. 7-11 October 2019 |
Year(s) Of Engagement Activity | 2019,2020 |
Description | Media activities for Nature Genetics paper (Viana et al) |
Form Of Engagement Activity | A press release, press conference or response to a media enquiry/interview |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Public/other audiences |
Results and Impact | Media interviews promoting our paper in Nature Genetics describing a single mutation requirement for a bacterial host shift. It involved radio and written press articles |
Year(s) Of Engagement Activity | 2015 |
Description | Media activities relating to AEM paper |
Form Of Engagement Activity | A press release, press conference or response to a media enquiry/interview |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Media (as a channel to the public) |
Results and Impact | Informed public and stimulated discussion feedback from general public |
Year(s) Of Engagement Activity | 2014 |
Description | Media activities relating to MBio publication |
Form Of Engagement Activity | A press release, press conference or response to a media enquiry/interview |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Public/other audiences |
Results and Impact | Informed public on research outputs and stimulated interest Stimulated interest and further enquiries from general public |
Year(s) Of Engagement Activity | 2013 |
Description | Media interviews for Nature Ecol evol paper |
Form Of Engagement Activity | A press release, press conference or response to a media enquiry/interview |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Public/other audiences |
Results and Impact | Talking to various media including print and radio regarding our Nature Ecology and Evolution publication |
Year(s) Of Engagement Activity | 2018 |